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FR2387243A1 - Procede pour purifier des extraits microbiens contenant des enzymes - Google Patents

Procede pour purifier des extraits microbiens contenant des enzymes

Info

Publication number
FR2387243A1
FR2387243A1 FR7737117A FR7737117A FR2387243A1 FR 2387243 A1 FR2387243 A1 FR 2387243A1 FR 7737117 A FR7737117 A FR 7737117A FR 7737117 A FR7737117 A FR 7737117A FR 2387243 A1 FR2387243 A1 FR 2387243A1
Authority
FR
France
Prior art keywords
extracts containing
containing enzymes
microbial extracts
purifying microbial
purifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7737117A
Other languages
English (en)
Other versions
FR2387243B1 (fr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eastman Kodak Co
Original Assignee
Eastman Kodak Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eastman Kodak Co filed Critical Eastman Kodak Co
Publication of FR2387243A1 publication Critical patent/FR2387243A1/fr
Application granted granted Critical
Publication of FR2387243B1 publication Critical patent/FR2387243B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/816—Enzyme separation or purification by solubility

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention est relative à la microbiologie. Le procédé suivant l'invention permet de purifier des extraits enzymatiques par précipitation des acides nucléides et des protéines indésirables grâce à l'addition à l'extrait enzymatique d'un polymère cationique soluble dans l'eau comprenant des motifs dérivés d'un monomère de formule :
FR7737117A 1976-12-10 1977-12-09 Procede pour purifier des extraits microbiens contenant des enzymes Granted FR2387243A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US05/749,647 US4055469A (en) 1976-12-10 1976-12-10 Purification of microbial enzyme extracts using synthetic polyelectrolytes

Publications (2)

Publication Number Publication Date
FR2387243A1 true FR2387243A1 (fr) 1978-11-10
FR2387243B1 FR2387243B1 (fr) 1981-12-04

Family

ID=25014606

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7737117A Granted FR2387243A1 (fr) 1976-12-10 1977-12-09 Procede pour purifier des extraits microbiens contenant des enzymes

Country Status (6)

Country Link
US (1) US4055469A (fr)
JP (1) JPS5372891A (fr)
CA (1) CA1095446A (fr)
DE (1) DE2755034C2 (fr)
FR (1) FR2387243A1 (fr)
GB (1) GB1590310A (fr)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4663288A (en) * 1985-05-22 1987-05-05 Nabisco Brands, Inc. Process for purification of enzymes
JPS62180865A (ja) * 1986-02-04 1987-08-08 Fujitsu Ltd 廃券スタツカ
US4873187A (en) * 1986-03-13 1989-10-10 Digene Diagnostics, Incorporated Bifunctional DNA-protein conjugating agent
US5047511A (en) * 1989-08-28 1991-09-10 Pitman-Moore, Inc. Method for recovering recombinant proteins
US5582988A (en) * 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5434270A (en) * 1994-09-15 1995-07-18 Eastman Kodak Company Weakly basic polymerizable monomers and polymers prepared therefrom
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
CN1232496A (zh) * 1996-08-12 1999-10-20 藤泽药品工业株式会社 制备生物反应器的方法
DE69839133T2 (de) * 1997-12-06 2009-02-05 Invitrogen Corp., Carlsbad Isolierung von Nukleinsäuren
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
DE69920711D1 (de) * 1998-07-21 2004-11-04 Monsanto Technology Llc Klärung von protein-niederschlagsuspensionen durch verwendung von anionischen polymerischen flockungsmitteln
WO2000044928A2 (fr) * 1999-01-27 2000-08-03 Halaka Folim G Matieres et procedes pour la purification de polyelectrolytes
CA2375060A1 (fr) 1999-05-24 2000-11-30 Invitrogen Corporation Procede de deblocage d'oligonucleotides marques
WO2002048385A2 (fr) * 2000-12-12 2002-06-20 Invitrogen Corporation Compositions et procedes pour liberer des molecules d'acide nucleique de matrices solides
CA2473376A1 (fr) 2002-01-16 2003-07-31 Dynal Biotech Asa Methode permettant d'isoler des acides nucleiques et des proteines contenus dans un echantillon unique
SE0202074D0 (sv) * 2002-06-28 2002-07-02 Amersham Biosciences Ab Isolation of nucleic acids
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US8163886B2 (en) * 2006-12-21 2012-04-24 Emd Millipore Corporation Purification of proteins
US8362217B2 (en) * 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
ES2374330T3 (es) * 2007-01-22 2012-02-15 Genentech, Inc. Precipitación con polielectrolito y purificación de anticuerpos.
WO2009151514A1 (fr) * 2008-06-11 2009-12-17 Millipore Corporation Bioréacteur à cuve agitée
JP2012511929A (ja) 2008-12-16 2012-05-31 イー・エム・デイー・ミリポア・コーポレイシヨン 攪拌タンク反応器及び方法
SG10201804385YA (en) 2010-05-17 2018-06-28 Emd Millipore Corp Stimulus responsive polymers for the purification of biomolecules
CN111849962A (zh) * 2019-04-25 2020-10-30 北京挑战农业科技有限公司 一种生物制剂中宿主残留rDNA的去除方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2048934A5 (fr) * 1969-05-29 1971-03-19 Monsanto Co
DE2159028A1 (de) * 1971-11-29 1973-05-30 Henkel & Cie Gmbh Verfahren zur klaerung von suspensionen biologischer materialien

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3252961A (en) * 1961-04-03 1966-05-24 Foremost Dairies Inc Whey process and product
US3846543A (en) * 1963-01-02 1974-11-05 Monsanto Co Process for the separation of virus from non-viral proteins
DE2001902C3 (de) * 1970-01-16 1978-10-12 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur Reinigung und Fraktionierung von gelösten aktiven Proteinen
US3728224A (en) * 1970-11-12 1973-04-17 Miles Lab Enzyme purification process
US3737377A (en) * 1971-01-27 1973-06-05 Miles Lab Purification of lactase
US3725545A (en) * 1971-02-03 1973-04-03 R Maes Enhancement of antibody production by nucleic acid-polycation complexes
IE38174B1 (en) * 1972-07-07 1978-01-18 Snam Progetti Process for purifying an enzyme

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2048934A5 (fr) * 1969-05-29 1971-03-19 Monsanto Co
DE2159028A1 (de) * 1971-11-29 1973-05-30 Henkel & Cie Gmbh Verfahren zur klaerung von suspensionen biologischer materialien

Also Published As

Publication number Publication date
GB1590310A (en) 1981-05-28
DE2755034A1 (de) 1978-06-15
JPS5372891A (en) 1978-06-28
FR2387243B1 (fr) 1981-12-04
JPS5651749B2 (fr) 1981-12-08
CA1095446A (fr) 1981-02-10
US4055469A (en) 1977-10-25
DE2755034C2 (de) 1982-06-24

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