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EA200001096A1 - Способ обогащения раствора аденовируса, способ точного подсчета числа аденовирусных частиц в образце раствора (варианты) и способ очистки аденовируса из клеток, инфицированных аденовирусом - Google Patents

Способ обогащения раствора аденовируса, способ точного подсчета числа аденовирусных частиц в образце раствора (варианты) и способ очистки аденовируса из клеток, инфицированных аденовирусом

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Publication number
EA200001096A1
EA200001096A1 EA200001096A EA200001096A EA200001096A1 EA 200001096 A1 EA200001096 A1 EA 200001096A1 EA 200001096 A EA200001096 A EA 200001096A EA 200001096 A EA200001096 A EA 200001096A EA 200001096 A1 EA200001096 A1 EA 200001096A1
Authority
EA
Eurasian Patent Office
Prior art keywords
adenovirus
solution
sample
dimethylaminopropyl
chromatographic resin
Prior art date
Application number
EA200001096A
Other languages
English (en)
Inventor
Мигель Э. Каррион
Имре Ковесди
Мэрилин Менджер
Original Assignee
Джинвек, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Джинвек, Инк. filed Critical Джинвек, Инк.
Publication of EA200001096A1 publication Critical patent/EA200001096A1/ru

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    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation
    • B01D15/345—Perfusive chromatography
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material
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    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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Abstract

Способ обогащения раствора аденовируса предусматривает нанесение смешанного раствора, содержащего аденовирус и, по меньшей мере, один нежелательный тип биомолекулы, на анионообменную хроматографическую смолу, содержащую связывающую структуру, выбранную из группы, состоящей из диметиламинопропила, диметиламинобутила диметиламиноизобутила и диметиламинопентила, и элюцию аденовируса с хроматографической смолы. Представлен также способ очистки аденовируса из инфицированных аденовирусом клеток, который включает лизирование данных клеток, нанесение лизата на одну хроматографическую смолу, элюцию аденовируса с хроматографической смолы и сбор фракции, содержащей аденовирус, который является практически таким же чистым, как аденовирус, трижды очищенный в градиенте плотности CsCl. Кроме того, данный способ представляет метод точного подсчета числа частиц аденовируса в растворе аденовируса, который предусматривает нанесение образца раствора аденовируса на анионообменную хроматографическую смолу и элюирование образца, сравнение поглощения образца раствора аденовируса и поглощения стандартного раствора аденовируса и подсчет числа аденовирусных частиц в образце раствора.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA200001096A 1998-04-22 1999-04-22 Способ обогащения раствора аденовируса, способ точного подсчета числа аденовирусных частиц в образце раствора (варианты) и способ очистки аденовируса из клеток, инфицированных аденовирусом EA200001096A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8262898P 1998-04-22 1998-04-22
PCT/US1999/008843 WO1999054441A1 (en) 1998-04-22 1999-04-22 Efficient purification of adenovirus

Publications (1)

Publication Number Publication Date
EA200001096A1 true EA200001096A1 (ru) 2001-04-23

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EA200001096A EA200001096A1 (ru) 1998-04-22 1999-04-22 Способ обогащения раствора аденовируса, способ точного подсчета числа аденовирусных частиц в образце раствора (варианты) и способ очистки аденовируса из клеток, инфицированных аденовирусом

Country Status (20)

Country Link
US (3) US6383795B1 (ru)
EP (1) EP1073721B1 (ru)
JP (1) JP2002512361A (ru)
AT (1) ATE303434T1 (ru)
AU (1) AU756889B2 (ru)
BG (1) BG104928A (ru)
BR (1) BR9909789A (ru)
CA (1) CA2328462C (ru)
DE (1) DE69927013T2 (ru)
EA (1) EA200001096A1 (ru)
EE (1) EE200000605A (ru)
HU (1) HUP0102209A2 (ru)
IL (1) IL139040A0 (ru)
IS (1) IS5673A (ru)
MX (1) MXPA00010338A (ru)
NO (1) NO20005295L (ru)
PL (1) PL343630A1 (ru)
SK (1) SK15682000A3 (ru)
WO (1) WO1999054441A1 (ru)
ZA (1) ZA200005363B (ru)

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NO20005295L (no) 2000-11-30
WO1999054441A1 (en) 1999-10-28
BG104928A (en) 2001-09-28
US6383795B1 (en) 2002-05-07
US20030203469A1 (en) 2003-10-30
MXPA00010338A (es) 2005-06-17
US20020034735A1 (en) 2002-03-21
US7141406B2 (en) 2006-11-28
AU3662099A (en) 1999-11-08
ATE303434T1 (de) 2005-09-15
ZA200005363B (en) 2001-09-25
JP2002512361A (ja) 2002-04-23
US6586226B2 (en) 2003-07-01
NO20005295D0 (no) 2000-10-20
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IS5673A (is) 2000-10-20
EE200000605A (et) 2002-04-15
AU756889B2 (en) 2003-01-23
HUP0102209A2 (hu) 2001-09-28
EP1073721A1 (en) 2001-02-07
DE69927013T2 (de) 2006-06-29
IL139040A0 (en) 2001-11-25
CA2328462A1 (en) 1999-10-28
CA2328462C (en) 2010-11-09
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