DK1989310T5 - System til fremstilling af aromatiske molekyler ved bioomdannelse - Google Patents
System til fremstilling af aromatiske molekyler ved bioomdannelse Download PDFInfo
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- DK1989310T5 DK1989310T5 DK07731065.4T DK07731065T DK1989310T5 DK 1989310 T5 DK1989310 T5 DK 1989310T5 DK 07731065 T DK07731065 T DK 07731065T DK 1989310 T5 DK1989310 T5 DK 1989310T5
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Classifications
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/42—Hydroxy-carboxylic acids
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
- C12Y101/03038—Vanillyl-alcohol oxidase (1.1.3.38)
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Claims (13)
1. Gær hørende til Saccharomyces cerevisiae transformeret af genet der koder for vanillylalkoholoxidase, hvilken sekvens er sekvensen SEQ ID NO. 1 eller en hvilken som helst sekvens med mindst 70%, fortrinsvis 80%, mere fortrinsvis 90%, identitet med sekvensen SEQ ID NO. 1.
2. Gær ifølge krav 1, kendetegnet ved at genet der koder for vanillylalkoholoxidase er indeholdt i et ekspressionssystem, hvilket system omfatter: (1) organ til integrering af systemet ind i gærens genom, omfattende til nukleotidsekvenser, (2) organ til at vælge gæren med det integrerede system, omfattende et selektions-insert omfattende to LoxP-sekvenser svarende til sekvensen SEQ ID NO. 3, grænsende op til en promotor, en selekterbar markør af genet med resistens over for antibiotikatyper, og en terminator, (3) en ekspressionskassette for genet der koder for vanillylalkoholoxidase, omfattende en promotor der tillader ekspressionen af genet, mindst ét kloningssted der tillader integreringen af genet, og en terminator.
3. Gær ifølge krav 2, kendetegnet ved at nucleotid-sekvenser for integration af expressionsystemet ind i genomet af gæren er TYlA-sekvenser svarende til sekvensen SEQ ID NO. 4 og TY1B sekvensen svarende til sekvensen SEQ ID NO. 5, af Saccharomyces cerevisiae, eller hvilke som helst sekvenser der er mindst 70%, fortrinsvis 80%, mere fortrinsvis 90%, identisk dermed.
4. Gæren ifølge et hvilket som helst af kravene 2 og 3, kendetegnet ved at promotoren der tillader ekspressionen af vanillylalkoholperoxidasen er promotoren af Saccharomyces cerevisiae TDH3-genet (SEQ ID NO. 6), og den forbundne terminator er terminatoren af Saccharomyces cerevisiae CYCl-genet, svarende til sekvensen SEQ ID NO. 7 eller hvilke som helst sekvenser der er mindst 70%, fortrinsvis 80%, mere fortrinsvis 90%, identisk dermed.
5. Gæren ifølge et hvilket som helst af kravene 2 til 4, kendetegnet ved at promotoren og terminatoren der tillader ekspressionen af den selekterbare markør er promotoren svarende til sekvensen SEQ ID NO. 8 og terminatoren svarende til sekvensen SEQ ID NO. 9 af Ashbya gossypii TEFl-genet eller hvilke som helst sekvenser der er mindst 70%, fortrinsvis 80%, mere fortrinsvis 90%, identisk dermed.
6. Gæren ifølge et hvilket som helst af kravene 2 til 5, kendetegnet ved at den selekterbare markør er et gen med resistens for antibiotika, valgt fra gruppen omfattende generne til resistens for geneticin, nourseothricin, phleomycin eller zeocin eller hvilke som helst andre gener med resistens for en dominant antibiotika overfor hvilken vild-type-gæren er følsom, og er fortrinsvis geneticin-resistens-genet svarende til sekvensen SEQ ID NO. 10 eller en hvilken som helst sekvens der er mindst 70%, fortrinsvis 80%, mere fortrinsvis 90%, identisk dermed.
7. Gæren ifølge et hvilket som helst af kravene 2 til 6, i hvilken sekvensen af ekspressionssystemet indeholdende vanillylalkoholoxidase er nukleotidsekvensen SEQ ID NO. 11 eller en hvilken som helst sekvens der er mindst 70%, fortrinsvis 80%, mere fortrinsvis 90%, identisk dermed.
8. Fremgangsmåde til fremstilling af naturlig coniferylalkohol og/eller naturlig ferulsyre, omfattende følgende trin: a) klone nukleotidsekvensen af genet der koder for vanillylalkoholoxidase ifølge krav 1, ind i ekspressionssystemet ifølge et af kravene 2 til 7, b) transformere gær hørende til Saccharomyces cerevisiae med det derved opnåede ekspressionssystem, c) dyrke gæren under betingelser der tillader ekspressionen af vanillylalkoholoxidasen, d) bringe gæren i kontakt med eugenol.
9. Fremgangsmåden ifølge krav 8, kendetegnet ved at den tillader produktionen af ferulsyre.
10. Fremgangsmåden ifølge et hvilket som helst af kravene 8 til 9, omfattende, efter trin b) og før trinnene c) og d), følgende trin bl), b2) og b3): bl) transformere gæren med excitionsvektoren valgt fra multikopivektorer, fortrinsvis pFL44s, omfattende sekvensen SEQ ID NO. 12 eller en hvilken som helst sekvens mindst 70%, fortrinsvis 80%, mere fortrinsvis 90%, identisk med sekvensen SEQ ID NO. 12, b2) dyrke gæren under betingelser der tillader ekspressionen af CRE-enzymet, b3) isolere gæren der har tabt den valgbare markør.
11. Fremgangsmåden ifølge krav 10, i hvilken trinnene b), bl), b2) og b3) gentages så mange gange som det ønskede antal kopier af ekspressionssystemet.
12. Fremgangsmåde til fremstilling af vanillin, omfattende trinnene a), b), c) og d) som beskrevet i et hvilket som helst af kravene 8 til 11 og et efterfølgende omdannelsestrin af ferulsyren og/eller coniferylalkoholen, fremstillet i trin d), til vanillin.
13. Fremgangsmåden til fremstilling af vanillin ifølge krav 12, i hvilken omdannelsestrinnet af ferulsyren og/eller coniferylalkoholen, fremstillet i trin d), til vanillin udføres i en gær- eller ved biokemisk proces eller enzymatisk proces.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0601838A FR2898131B1 (fr) | 2006-03-01 | 2006-03-01 | Systeme de production de molecules aromatiques par bioconversion |
| PCT/FR2007/000364 WO2007099230A2 (fr) | 2006-03-01 | 2007-02-28 | Systeme d ' expression chez la levure pour la production de molecules aromatiques |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK1989310T3 DK1989310T3 (da) | 2016-02-08 |
| DK1989310T5 true DK1989310T5 (da) | 2016-02-22 |
Family
ID=37450756
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK07731065.4T DK1989310T5 (da) | 2006-03-01 | 2007-02-28 | System til fremstilling af aromatiske molekyler ved bioomdannelse |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US8404472B2 (da) |
| EP (1) | EP1989310B1 (da) |
| JP (1) | JP5350806B2 (da) |
| CN (1) | CN101432429B (da) |
| DK (1) | DK1989310T5 (da) |
| ES (1) | ES2560430T3 (da) |
| FR (1) | FR2898131B1 (da) |
| PL (1) | PL1989310T3 (da) |
| PT (1) | PT1989310E (da) |
| WO (1) | WO2007099230A2 (da) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2912758B1 (fr) * | 2007-02-21 | 2009-05-15 | Mane Fils Sa V | Systeme de production de molecules aromatiques chez streptomyces. |
| DE102009020299A1 (de) * | 2009-05-07 | 2010-11-11 | Clariant International Ltd. | Kammpolymere und deren Verwendung in Wasch- und Reinigungsmitteln |
| JP5655416B2 (ja) * | 2010-07-30 | 2015-01-21 | ユーハ味覚糖株式会社 | 新規フラバン化合物 |
| US9932610B2 (en) * | 2013-11-04 | 2018-04-03 | Bgn Tech Llc | Methods of making vanillin via the microbial fermentation of ferulic acid from eugenol using a plant dehydrogenase |
| WO2016016805A1 (en) * | 2014-08-01 | 2016-02-04 | Versalis S.P.A. | Gene construct for the transformation of yeast strains |
| US10451435B2 (en) * | 2016-06-03 | 2019-10-22 | Panasonic Automotive Systems Company of America, Division of Panasonic of North American | Method of using GPS map information to highlight road markings on a head up display that otherwise may be non-visible due to inclement weather |
| KR20200135469A (ko) * | 2018-03-27 | 2020-12-02 | 바스프 에스이 | 자일로스 대사 효모 |
| CN113136347B (zh) * | 2020-01-20 | 2022-06-03 | 暨南大学 | 高产松柏醇的酿酒酵母工程菌及其构建和应用 |
| EP3922727A1 (en) | 2020-06-12 | 2021-12-15 | Basf Se | Method for separating biomass from a solution comprising biomass and at least one aroma compound |
| CN112921049B (zh) * | 2021-02-06 | 2024-01-23 | 石河子大学 | 一种用于生产香草醛的基因片段、酿酒酵母工程菌及其构建方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6306625B1 (en) * | 1988-12-30 | 2001-10-23 | Smithkline Beecham Biologicals, Sa | Method for obtaining expression of mixed polypeptide particles in yeast |
| FR2661189B1 (fr) | 1990-04-19 | 1994-11-18 | Pernod Ricard | Production de vanilline par bioconversion de precurseurs benzeniques. |
| FR2694020B1 (fr) | 1992-07-24 | 1994-10-14 | Mane Fils Sa V | Procédé de préparation de substances aromatiques par voie enzymatique. |
| FR2724394B1 (fr) * | 1994-09-13 | 1997-01-10 | Agronomique Inst Nat Rech | Procede d'obtention d'acide vanillique et de vanilline par bioconversion par une association de microorganismes filamenteux |
| FR2729661B1 (fr) | 1995-01-19 | 1997-04-04 | Mane Fils V | Procede de preparation de substances aromatiques par voie biochimique |
| FR2792334B1 (fr) | 1999-04-14 | 2003-03-07 | Agronomique Inst Nat Rech | Procede d'obtention de cultures d'a. niger et leurs utilisations pour la production d'acide ferulique et d'acide vanillique |
| US6818752B2 (en) * | 2000-01-31 | 2004-11-16 | Biocatalytics, Inc. | Synthetic genes for enhanced expression |
-
2006
- 2006-03-01 FR FR0601838A patent/FR2898131B1/fr active Active
-
2007
- 2007-02-28 PL PL07731065T patent/PL1989310T3/pl unknown
- 2007-02-28 CN CN2007800158066A patent/CN101432429B/zh active Active
- 2007-02-28 US US12/281,405 patent/US8404472B2/en active Active
- 2007-02-28 EP EP07731065.4A patent/EP1989310B1/fr active Active
- 2007-02-28 DK DK07731065.4T patent/DK1989310T5/da active
- 2007-02-28 ES ES07731065.4T patent/ES2560430T3/es active Active
- 2007-02-28 WO PCT/FR2007/000364 patent/WO2007099230A2/fr active Application Filing
- 2007-02-28 JP JP2008556817A patent/JP5350806B2/ja active Active
- 2007-02-28 PT PT77310654T patent/PT1989310E/pt unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP1989310B1 (fr) | 2015-11-04 |
| WO2007099230A3 (fr) | 2008-04-17 |
| PL1989310T3 (pl) | 2016-05-31 |
| DK1989310T3 (da) | 2016-02-08 |
| US8404472B2 (en) | 2013-03-26 |
| FR2898131B1 (fr) | 2012-08-31 |
| FR2898131A1 (fr) | 2007-09-07 |
| US20100028963A1 (en) | 2010-02-04 |
| PT1989310E (pt) | 2016-03-02 |
| CN101432429B (zh) | 2013-08-07 |
| EP1989310A2 (fr) | 2008-11-12 |
| JP5350806B2 (ja) | 2013-11-27 |
| ES2560430T3 (es) | 2016-02-19 |
| WO2007099230A2 (fr) | 2007-09-07 |
| CN101432429A (zh) | 2009-05-13 |
| JP2009528041A (ja) | 2009-08-06 |
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