DK175898B1 - Fremgangsmåde til at udtrykke ikke-gærprotein i en gærvært og transformeret gærvært samt anvendelsen af en transformeret gær til fremstilling af gærværten - Google Patents

Fremgangsmåde til at udtrykke ikke-gærprotein i en gærvært og transformeret gærvært samt anvendelsen af en transformeret gær til fremstilling af gærværten Download PDF

Info

Publication number: DK175898B1
Authority: DK; Denmark
Prior art keywords: yeast; adr; promoter; expression cassette; host
Prior art date: 1988-05-06

Application number

DK198902215A

Other languages

Danish (da)

English (en)

Other versions

DK221589D0 (da

DK221589A (da

Inventor

Jeffrey R Shuster

Original Assignee

Chiron Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1988-05-06

Filing date

1989-05-05

Publication date

2005-05-30

1989-05-05 Application filed by Chiron Corp filed Critical Chiron Corp

1989-05-05 Publication of DK221589D0 publication Critical patent/DK221589D0/da

1989-11-07 Publication of DK221589A publication Critical patent/DK221589A/da

2005-05-30 Application granted granted Critical

2005-05-30 Publication of DK175898B1 publication Critical patent/DK175898B1/da

Links

240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 146
238000000034 method Methods 0.000 title claims abstract description 19
108090000623 proteins and genes Proteins 0.000 title description 40
102000004169 proteins and genes Human genes 0.000 title description 28
230000014509 gene expression Effects 0.000 claims abstract description 63
102100034044 All-trans-retinol dehydrogenase [NAD(+)] ADH1B Human genes 0.000 claims abstract description 31
101710193111 All-trans-retinol dehydrogenase [NAD(+)] ADH4 Proteins 0.000 claims abstract description 31
108010058643 Fungal Proteins Proteins 0.000 claims abstract description 21
239000013612 plasmid Substances 0.000 claims description 37
108091026890 Coding region Proteins 0.000 claims description 34
239000013598 vector Substances 0.000 claims description 29
230000005026 transcription initiation Effects 0.000 claims description 23
102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 claims description 20
108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 claims description 20
230000005030 transcription termination Effects 0.000 claims description 18
238000011144 upstream manufacturing Methods 0.000 claims description 15
101100490563 Caenorhabditis elegans adr-1 gene Proteins 0.000 claims description 14
230000004913 activation Effects 0.000 claims description 11
101150022075 ADR1 gene Proteins 0.000 claims description 10
108091028043 Nucleic acid sequence Proteins 0.000 claims description 8
238000004519 manufacturing process Methods 0.000 claims description 6
230000008569 process Effects 0.000 claims description 2
238000011084 recovery Methods 0.000 claims 1
230000001105 regulatory effect Effects 0.000 abstract description 12
210000004027 cell Anatomy 0.000 description 21
108020004414 DNA Proteins 0.000 description 20
239000012634 fragment Substances 0.000 description 11
108091029865 Exogenous DNA Proteins 0.000 description 8
101710187573 Alcohol dehydrogenase 2 Proteins 0.000 description 7
108020004705 Codon Proteins 0.000 description 7
230000010354 integration Effects 0.000 description 7
230000035897 transcription Effects 0.000 description 7
238000013518 transcription Methods 0.000 description 7
238000010276 construction Methods 0.000 description 6
230000000694 effects Effects 0.000 description 6
BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Natural products O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 6
230000001965 increasing effect Effects 0.000 description 6
239000000463 material Substances 0.000 description 6
108020004999 messenger RNA Proteins 0.000 description 6
102000053602 DNA Human genes 0.000 description 5
210000000349 chromosome Anatomy 0.000 description 5
239000013604 expression vector Substances 0.000 description 5
229920001184 polypeptide Polymers 0.000 description 5
102000004196 processed proteins & peptides Human genes 0.000 description 5
108090000765 processed proteins & peptides Proteins 0.000 description 5
230000010076 replication Effects 0.000 description 5
238000010561 standard procedure Methods 0.000 description 5
230000009466 transformation Effects 0.000 description 5
238000013519 translation Methods 0.000 description 5
101150078509 ADH2 gene Proteins 0.000 description 4
102000048143 Insulin-Like Growth Factor II Human genes 0.000 description 4
108090001117 Insulin-Like Growth Factor II Proteins 0.000 description 4
241000235070 Saccharomyces Species 0.000 description 4
102000019197 Superoxide Dismutase Human genes 0.000 description 4
108010012715 Superoxide dismutase Proteins 0.000 description 4
101150050575 URA3 gene Proteins 0.000 description 4
238000010367 cloning Methods 0.000 description 4
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 3
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 3
102000004190 Enzymes Human genes 0.000 description 3
108090000790 Enzymes Proteins 0.000 description 3
241000725303 Human immunodeficiency virus Species 0.000 description 3
241000235649 Kluyveromyces Species 0.000 description 3
ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 3
150000001413 amino acids Chemical class 0.000 description 3
230000033228 biological regulation Effects 0.000 description 3
230000002068 genetic effect Effects 0.000 description 3
241000222120 Candida <Saccharomycetales> Species 0.000 description 2
241000588724 Escherichia coli Species 0.000 description 2
241000233866 Fungi Species 0.000 description 2
ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 2
108091034117 Oligonucleotide Proteins 0.000 description 2
241000235648 Pichia Species 0.000 description 2
LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 2
108020004511 Recombinant DNA Proteins 0.000 description 2
241000235346 Schizosaccharomyces Species 0.000 description 2
108091081024 Start codon Proteins 0.000 description 2
IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
108700009124 Transcription Initiation Site Proteins 0.000 description 2
241000700605 Viruses Species 0.000 description 2
230000001580 bacterial effect Effects 0.000 description 2
230000002759 chromosomal effect Effects 0.000 description 2
OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
230000008021 deposition Effects 0.000 description 2
230000004927 fusion Effects 0.000 description 2
UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
238000009396 hybridization Methods 0.000 description 2
238000001727 in vivo Methods 0.000 description 2
238000012423 maintenance Methods 0.000 description 2
244000005700 microbiome Species 0.000 description 2
238000010369 molecular cloning Methods 0.000 description 2
230000035772 mutation Effects 0.000 description 2
239000002773 nucleotide Substances 0.000 description 2
125000003729 nucleotide group Chemical group 0.000 description 2
108091008146 restriction endonucleases Proteins 0.000 description 2
241000894007 species Species 0.000 description 2
230000001131 transforming effect Effects 0.000 description 2
FQVLRGLGWNWPSS-BXBUPLCLSA-N (4r,7s,10s,13s,16r)-16-acetamido-13-(1h-imidazol-5-ylmethyl)-10-methyl-6,9,12,15-tetraoxo-7-propan-2-yl-1,2-dithia-5,8,11,14-tetrazacycloheptadecane-4-carboxamide Chemical compound N1C(=O)[C@@H](NC(C)=O)CSSC[C@@H](C(N)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]1CC1=CN=CN1 FQVLRGLGWNWPSS-BXBUPLCLSA-N 0.000 description 1
229930024421 Adenine Natural products 0.000 description 1
GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
101710187578 Alcohol dehydrogenase 1 Proteins 0.000 description 1
102100034035 Alcohol dehydrogenase 1A Human genes 0.000 description 1
108700028369 Alleles Proteins 0.000 description 1
102100034613 Annexin A2 Human genes 0.000 description 1
108090000668 Annexin A2 Proteins 0.000 description 1
DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
241000235172 Bullera Species 0.000 description 1
108020004635 Complementary DNA Proteins 0.000 description 1
108091035707 Consensus sequence Proteins 0.000 description 1
241000490729 Cryptococcaceae Species 0.000 description 1
241000221199 Cryptococcus <basidiomycete yeast> Species 0.000 description 1
230000004543 DNA replication Effects 0.000 description 1
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
102000018233 Fibroblast Growth Factor Human genes 0.000 description 1
108050007372 Fibroblast Growth Factor Proteins 0.000 description 1
241000221207 Filobasidium Species 0.000 description 1
108700007698 Genetic Terminator Regions Proteins 0.000 description 1
101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
101000780443 Homo sapiens Alcohol dehydrogenase 1A Proteins 0.000 description 1
108010020056 Hydrogenase Proteins 0.000 description 1
101150062179 II gene Proteins 0.000 description 1
241001138401 Kluyveromyces lactis Species 0.000 description 1
102000003960 Ligases Human genes 0.000 description 1
108090000364 Ligases Proteins 0.000 description 1
241000124008 Mammalia Species 0.000 description 1
101710163270 Nuclease Proteins 0.000 description 1
101710149086 Nuclease S1 Proteins 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
102000009569 Phosphoglucomutase Human genes 0.000 description 1
102000012288 Phosphopyruvate Hydratase Human genes 0.000 description 1
108010022181 Phosphopyruvate Hydratase Proteins 0.000 description 1
102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
102000001253 Protein Kinase Human genes 0.000 description 1
108700005075 Regulator Genes Proteins 0.000 description 1
241000223252 Rhodotorula Species 0.000 description 1
244000206963 Saccharomyces cerevisiae var. diastaticus Species 0.000 description 1
241001407717 Saccharomyces norbensis Species 0.000 description 1
241000235344 Saccharomycetaceae Species 0.000 description 1
241000235343 Saccharomycetales Species 0.000 description 1
241000228389 Sporidiobolus Species 0.000 description 1
241000222068 Sporobolomyces <Sporidiobolaceae> Species 0.000 description 1
240000006345 Trifolium hybridum Species 0.000 description 1
102000005924 Triose-Phosphate Isomerase Human genes 0.000 description 1
108700015934 Triose-phosphate isomerases Proteins 0.000 description 1
108020005202 Viral DNA Proteins 0.000 description 1
238000007792 addition Methods 0.000 description 1
229960000643 adenine Drugs 0.000 description 1
150000001408 amides Chemical class 0.000 description 1
230000008901 benefit Effects 0.000 description 1
IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
239000012620 biological material Substances 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
230000008859 change Effects 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
239000002299 complementary DNA Substances 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
238000012258 culturing Methods 0.000 description 1
238000005520 cutting process Methods 0.000 description 1
229940104302 cytosine Drugs 0.000 description 1
239000005547 deoxyribonucleotide Substances 0.000 description 1
125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
238000000502 dialysis Methods 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
239000003623 enhancer Substances 0.000 description 1
238000012869 ethanol precipitation Methods 0.000 description 1
230000001747 exhibiting effect Effects 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
238000000605 extraction Methods 0.000 description 1
229940126864 fibroblast growth factor Drugs 0.000 description 1
108020001507 fusion proteins Proteins 0.000 description 1
102000037865 fusion proteins Human genes 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
239000008103 glucose Substances 0.000 description 1
230000034659 glycolysis Effects 0.000 description 1
230000002414 glycolytic effect Effects 0.000 description 1
210000000003 hoof Anatomy 0.000 description 1
210000001822 immobilized cell Anatomy 0.000 description 1
230000002779 inactivation Effects 0.000 description 1
230000001939 inductive effect Effects 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
238000003780 insertion Methods 0.000 description 1
230000037431 insertion Effects 0.000 description 1
230000002262 irrigation Effects 0.000 description 1
238000003973 irrigation Methods 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
238000013507 mapping Methods 0.000 description 1
239000003550 marker Substances 0.000 description 1
230000011278 mitosis Effects 0.000 description 1
239000000203 mixture Substances 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000007899 nucleic acid hybridization Methods 0.000 description 1
150000007523 nucleic acids Chemical group 0.000 description 1
235000015097 nutrients Nutrition 0.000 description 1
238000002515 oligonucleotide synthesis Methods 0.000 description 1
238000012261 overproduction Methods 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
108091000115 phosphomannomutase Proteins 0.000 description 1
238000007747 plating Methods 0.000 description 1
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
108060006633 protein kinase Proteins 0.000 description 1
230000002797 proteolythic effect Effects 0.000 description 1
229940107700 pyruvic acid Drugs 0.000 description 1
108091035233 repetitive DNA sequence Proteins 0.000 description 1
102000053632 repetitive DNA sequence Human genes 0.000 description 1
230000003362 replicative effect Effects 0.000 description 1
239000013605 shuttle vector Substances 0.000 description 1
238000000638 solvent extraction Methods 0.000 description 1
239000007858 starting material Substances 0.000 description 1
210000000130 stem cell Anatomy 0.000 description 1
229940104230 thymidine Drugs 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
230000002588 toxic effect Effects 0.000 description 1
230000001988 toxicity Effects 0.000 description 1
231100000419 toxicity Toxicity 0.000 description 1
210000005253 yeast cell Anatomy 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/50—Fibroblast growth factor [FGF]
- C07K14/503—Fibroblast growth factor [FGF] basic FGF [bFGF]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Mycology (AREA)
Biophysics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Toxicology (AREA)
Gastroenterology & Hepatology (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)

DK198902215A 1988-05-06 1989-05-05 Fremgangsmåde til at udtrykke ikke-gærprotein i en gærvært og transformeret gærvært samt anvendelsen af en transformeret gær til fremstilling af gærværten DK175898B1 (da)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US19086888A	1988-05-06	1988-05-06
US19086888		1988-05-06

Publications (3)

Publication Number	Publication Date
DK221589D0 DK221589D0 (da)	1989-05-05
DK221589A DK221589A (da)	1989-11-07
DK175898B1 true DK175898B1 (da)	2005-05-30

Family

ID=22703135

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK198902215A DK175898B1 (da)	1988-05-06	1989-05-05	Fremgangsmåde til at udtrykke ikke-gærprotein i en gærvært og transformeret gærvært samt anvendelsen af en transformeret gær til fremstilling af gærværten

Country Status (8)

Country	Link
US (1)	US6183985B1 (ja)
EP (1)	EP0340986B1 (ja)
JP (4)	JP3074174B2 (ja)
AT (1)	ATE123521T1 (ja)
CA (1)	CA1324969C (ja)
DE (1)	DE68922932T2 (ja)
DK (1)	DK175898B1 (ja)
ES (1)	ES2072900T3 (ja)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP3732516B2 (ja) *	1995-02-09	2006-01-05	ノバルティス・アクチエンゲゼルシャフト	タンパク質の製造法
DE19544233A1 (de) *	1995-11-28	1997-06-05	Hoechst Ag	Verfahren zur Nutzung des Hefe-ADH II-Promotorsystems zur biotechnologischen Produktion heterologer Proteine in hohen Ausbeuten
WO2003027280A1 (fr) *	2001-09-20	2003-04-03	Toyota Jidosha Kabushiki Kaisha	Systeme de surexpression genique
CA2553034A1 (en)	2004-02-02	2005-08-18	Ambrx, Inc.	Modified human interferon polypeptides and their uses
CA2568952C (en) *	2004-06-18	2019-05-21	Ambrx, Inc.	Novel antigen-binding polypeptides and their uses
AU2005327906B2 (en) *	2004-07-21	2010-05-13	Ambrx, Inc.	Biosynthetic polypeptides utilizing non-naturally encoded amino acids
CN104803865A (zh)	2004-12-22	2015-07-29	Ambrx公司	含有非天然氨基酸和多肽的组合物、涉及非天然氨基酸和多肽的方法以及其用途
US7736872B2 (en) *	2004-12-22	2010-06-15	Ambrx, Inc.	Compositions of aminoacyl-TRNA synthetase and uses thereof
KR101224781B1 (ko)	2004-12-22	2013-01-21	암브룩스, 인코포레이티드	비천연적으로 코딩된 아미노산을 포함하는 인간 성장호르몬의 조제물
JP2008525032A (ja)	2004-12-22	2008-07-17	アンブレツクス・インコーポレイテツド	組換えヒト成長ホルモンを発現及び精製するための方法
EP1836314B1 (en) *	2004-12-22	2012-01-25	Ambrx, Inc.	Modified human growth hormone
EP1893229B1 (en) *	2005-06-03	2011-10-19	Ambrx, Inc.	Improved human interferon molecules and their uses
DE102005029170A1 (de) *	2005-06-23	2006-12-28	Consortium für elektrochemische Industrie GmbH	System zur Genexpression in Hefen der Gattung Sporidiobolus
CN103103238B (zh)	2005-08-18	2016-08-10	Ambrx公司	一种在细胞中制造在特定位置处具有所选氨基酸的抗体或抗体片段多肽的方法
JP5508716B2 (ja) *	2005-11-08	2014-06-04	アンブルックス，インコーポレイテッド	非天然アミノ酸、および非天然アミノ酸ポリペプチドを修飾するための促進剤
CN101454461A (zh)	2005-11-16	2009-06-10	Ambrx公司	包括非天然氨基酸的方法和组合物
JP5215865B2 (ja)	2005-11-22	2013-06-19	ノバルティスヴァクシンズアンドダイアグノスティクスインコーポレイテッド	ノロウイルス抗原およびサポウイルス抗原
WO2008030558A2 (en)	2006-09-08	2008-03-13	Ambrx, Inc.	Modified human plasma polypeptide or fc scaffolds and their uses
JP5451390B2 (ja) *	2006-09-08	2014-03-26	アンブルックス，インコーポレイテッド	脊椎動物細胞内におけるサプレッサーｔｒｎａの転写
SG174781A1 (en) *	2006-09-08	2011-10-28	Ambrx Inc	Hybrid suppressor trna for vertebrate cells
US8143023B2 (en)	2007-01-26	2012-03-27	Novozymes A/S	Method for methanol independent induction from methanol inducible promoters in Pichia
ES2385114T3 (es)	2007-03-30	2012-07-18	Ambrx, Inc.	Polipéptidos de FGF-21 modificados y sus usos
NZ580686A (en)	2007-05-02	2012-11-30	Ambrx Inc	Modified interferon beta polypeptides and their uses
NZ603812A (en)	2007-11-20	2014-06-27	Ambrx Inc	Modified insulin polypeptides and their uses
NZ586947A (en)	2008-02-08	2012-11-30	Ambrx Inc	Modified leptin polypeptides and their uses
WO2010004042A2 (en) *	2008-07-11	2010-01-14	Novozymes A/S	Pichia pastoris das promoter variants
CN106928339A (zh)	2008-07-23	2017-07-07	Ambrx 公司	经修饰的牛g‑csf多肽和其用途
BRPI0919031A2 (pt)	2008-09-26	2014-09-23	Ambrx Inc	Micro-organismos e vacinas dependentes de replicação através do uso de aminoácidos não naturais
CN107022020A (zh)	2008-09-26	2017-08-08	Ambrx公司	修饰的动物促红细胞生成素多肽和其用途
MX2012006980A (es)	2009-12-21	2012-07-17	Ambrx Inc	Polipeptidos de somatotropina porcina modificados y sus usos.
EP2805964A1 (en)	2009-12-21	2014-11-26	Ambrx, Inc.	Modified bovine somatotropin polypeptides and their uses
JP2013533745A (ja)	2010-07-06	2013-08-29	ノバルティスアーゲー	ノロウイルスに由来する免疫原性組成物および方法
US9567386B2 (en)	2010-08-17	2017-02-14	Ambrx, Inc.	Therapeutic uses of modified relaxin polypeptides
PT2605789T (pt)	2010-08-17	2019-09-03	Ambrx Inc	Polipeptidos de relaxina modificados e suas utilizações
AR083006A1 (es)	2010-09-23	2013-01-23	Lilly Co Eli	Formulaciones para el factor estimulante de colonias de granulocitos (g-csf) bovino y variantes de las mismas
EP3209316A2 (en)	2014-10-24	2017-08-30	Bristol-Myers Squibb Company	Modified fgf-21 polypeptides and uses thereof
US10266578B2 (en)	2017-02-08	2019-04-23	Bristol-Myers Squibb Company	Modified relaxin polypeptides comprising a pharmacokinetic enhancer and uses thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4880734A (en) *	1984-05-11	1989-11-14	Chiron Corporation	Eukaryotic regulatable transcription
ATE63757T1 (de) *	1985-03-28	1991-06-15	Chiron Corp	Expression durch verwendung von fusionsgenen fuer proteinproduktion.
EP0234871A3 (en)	1986-02-28	1988-10-12	Merck & Co. Inc.	Regulatable expression of recombinant proteins in yeast
US5068185A (en)	1986-07-10	1991-11-26	Merck & Co., Inc.	Trains of yeast for the expression of heterologous genes

1989
- 1989-04-21 CA CA000597461A patent/CA1324969C/en not_active Expired - Lifetime
- 1989-04-28 ES ES89304297T patent/ES2072900T3/es not_active Expired - Lifetime
- 1989-04-28 EP EP89304297A patent/EP0340986B1/en not_active Expired - Lifetime
- 1989-04-28 AT AT89304297T patent/ATE123521T1/de not_active IP Right Cessation
- 1989-04-28 DE DE68922932T patent/DE68922932T2/de not_active Expired - Lifetime
- 1989-05-02 JP JP01113585A patent/JP3074174B2/ja not_active Expired - Lifetime
- 1989-05-05 DK DK198902215A patent/DK175898B1/da not_active IP Right Cessation
1995
- 1995-03-01 US US08/397,553 patent/US6183985B1/en not_active Expired - Lifetime
2000
- 2000-01-12 JP JP2000004098A patent/JP3484386B2/ja not_active Expired - Lifetime
2002
- 2002-08-14 JP JP2002236635A patent/JP3561513B2/ja not_active Expired - Fee Related
2003
- 2003-12-10 JP JP2003412625A patent/JP2004081222A/ja not_active Withdrawn

Also Published As

Publication number	Publication date
DE68922932T2 (de)	1995-10-19
ES2072900T3 (es)	1995-08-01
JP3484386B2 (ja)	2004-01-06
EP0340986B1 (en)	1995-06-07
EP0340986A3 (en)	1991-07-31
ATE123521T1 (de)	1995-06-15
JP3561513B2 (ja)	2004-09-02
JP2004081222A (ja)	2004-03-18
JP2000300277A (ja)	2000-10-31
DK221589D0 (da)	1989-05-05
DE68922932D1 (de)	1995-07-13
EP0340986A2 (en)	1989-11-08
CA1324969C (en)	1993-12-07
JPH02104291A (ja)	1990-04-17
US6183985B1 (en)	2001-02-06
JP2003116525A (ja)	2003-04-22
DK221589A (da)	1989-11-07
JP3074174B2 (ja)	2000-08-07

Publication	Publication Date	Title
DK175898B1 (da)	2005-05-30	Fremgangsmåde til at udtrykke ikke-gærprotein i en gærvært og transformeret gærvært samt anvendelsen af en transformeret gær til fremstilling af gærværten
US5674706A (en)	1997-10-07	High level expression of proteins in yeast
CA1340772C (en)	1999-09-28	Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
FI81379C (fi)	1990-10-10	Anvaendning av kluyveromyces-jaest saosom vaerd foer transformering och uttryckning av fraemmande gener.
Hansen et al.	1990	Characterization of a transpositionally active Ty3 element and identification of the Ty3 integrase protein
Jia et al.	1997	The CIT3 gene of Saccharomyces cerevisiae encodes a second mitochondrial isoform of citrate synthase
Filho et al.	1986	Stable Yeast Transformants that Secrete Functional α–Amylase Encoded by Cloned Mouse Pancreatic cDNA
Nishi et al.	1995	The GCR1 requirement for yeast glycolytic gene expression is suppressed by dominant mutations in the SGC1 gene, which encodes a novel basic-helix-loop-helix protein
EP0329684A1 (en)	1989-08-30	Composite yeast vectors
JP4439398B2 (ja)	2010-03-24	減数分裂組換えの標的化刺激を誘導する方法及び前記方法を実施するためのキット
WO1993018167A1 (en)	1993-09-16	A method for production of proteins in yeast
Icho et al.	1987	Metal-binding, nucleic acid-binding finger sequences in the CDC16 gene of Saccharomyces cerevisiae
KR20010020482A (ko)	2001-03-15	개량된 단백질 발현균주
DK176000B1 (da)	2005-11-14	Fremgangsmåde til fremstilling af et protein ud fra gærsvampe under anvendelse af et inducerbart system, samt vektorer og transformerede stammer til brug ved fremgangsmåden
Munholland et al.	1990	DNA sequences required for yeast actin gene transcription do not include conserved CCAAT motifs
JPH06165691A (ja)	1994-06-14	プロテアーゼ阻害剤の生産方法
JP4587750B2 (ja)	2010-11-24	転写活性化タンパク質をコードするキャンディダユティリス由来の遺伝子
JP6112548B2 (ja)	2017-04-12	自己倍数化抑制に基づく酵母の育種方法
Phillips	1991	Isolation and characterization of QCR9 the nuclear gene encoding the 7.3 kDa subunit 9 of the Saccharomyces cerevisiae bc (1) complex
Greve	1991	Identification Of Cis-Acting Control Sequences For The Developmentally Regulated brlA Gene In Aspergillus Nidulans
JPH03262487A (ja)	1991-11-22	ヒト血清アルブミンの安定な製造方法
JPH02502964A (ja)	1990-09-20	Ｌｅｕ３を用いるアミノ酸合成の調節

Legal Events

Date	Code	Title	Description
2009-05-25	PUP	Patent expired