[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

DK1481057T3 - System og fremgangsmåde til fremstilling af rekombinante glycosylerede proteiner i en prokaryot vært - Google Patents

System og fremgangsmåde til fremstilling af rekombinante glycosylerede proteiner i en prokaryot vært

Info

Publication number
DK1481057T3
DK1481057T3 DK03702276T DK03702276T DK1481057T3 DK 1481057 T3 DK1481057 T3 DK 1481057T3 DK 03702276 T DK03702276 T DK 03702276T DK 03702276 T DK03702276 T DK 03702276T DK 1481057 T3 DK1481057 T3 DK 1481057T3
Authority
DK
Denmark
Prior art keywords
prokaryotic host
producing recombinant
glycosylated proteins
recombinant glycosylated
oligosaccharide
Prior art date
Application number
DK03702276T
Other languages
Danish (da)
English (en)
Inventor
Markus Aebi
Michael Wacker
Original Assignee
Eidgenoess Tech Hochschule
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eidgenoess Tech Hochschule filed Critical Eidgenoess Tech Hochschule
Application granted granted Critical
Publication of DK1481057T3 publication Critical patent/DK1481057T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/005Glycopeptides, glycoproteins
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/02Bacterial antigens
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/54Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
    • A61K47/549Sugars, nucleosides, nucleotides or nucleic acids
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
    • C07K16/121Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Helicobacter (Campylobacter) (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/90Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK03702276T 2002-03-07 2003-03-05 System og fremgangsmåde til fremstilling af rekombinante glycosylerede proteiner i en prokaryot vært DK1481057T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH3942002 2002-03-07
US36465502P 2002-03-14 2002-03-14

Publications (1)

Publication Number Publication Date
DK1481057T3 true DK1481057T3 (da) 2006-05-15

Family

ID=27789602

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03702276T DK1481057T3 (da) 2002-03-07 2003-03-05 System og fremgangsmåde til fremstilling af rekombinante glycosylerede proteiner i en prokaryot vært

Country Status (12)

Country Link
US (3) US20050287628A1 (es)
EP (1) EP1481057B1 (es)
KR (1) KR20090110951A (es)
AT (1) ATE317897T1 (es)
AU (1) AU2003205502C1 (es)
CA (1) CA2477794C (es)
DE (1) DE60303591T2 (es)
DK (1) DK1481057T3 (es)
ES (1) ES2258214T3 (es)
IL (3) IL163806A0 (es)
PT (1) PT1481057E (es)
WO (1) WO2003074687A1 (es)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL163806A0 (en) * 2002-03-07 2005-12-18 Eidgenoess Tech Hochschule System and method for the production of recombinant glycosylated proteins in a prokaryotic host
AU2011218682B2 (en) * 2005-05-11 2013-11-21 Eth Zurich Recombinant N-glycosylated proteins from procaryotic cells
WO2006119987A2 (en) * 2005-05-11 2006-11-16 ETH Zürich Recombinant n-glycosylated proteins from procaryotic cells
JP5667880B2 (ja) 2008-01-03 2015-02-12 コーネル リサーチ ファンデーション インコーポレイテッド 原核生物におけるグリコシル化されたタンパク質の発現方法
HUE039413T2 (hu) 2008-02-20 2018-12-28 Glaxosmithkline Biologicals Sa Prokarióta sejtekbõl származó rekombináns N-glikozilált fehérjékbõl készített biokonjugátumok
LT2501406T (lt) 2009-11-19 2018-03-26 Glaxosmithkline Biologicals S.A. Biosintetinė sistema sukurianti imunogeninius polisacharidus prokariotinėse gardelėse
AU2011249839B2 (en) 2010-05-06 2016-05-26 Glaxosmithkline Biologicals S.A. Capsular gram-positive bacteria bioconjugate vaccines
AU2012297533B2 (en) 2011-08-12 2016-02-25 Instituto De Investigaciones Biotecnologicas-Instituto Tecnologico De Chascomus Consejo De Investigaciones Cientificas Y Tecnicas, Universidad Nacional De San Martin Method of diagnosing bacterial infections using bacterial glycoproteins
EP2753353A1 (en) 2011-09-06 2014-07-16 Glycovaxyn AG Bioconjugate vaccines made in prokaryotic cells
CN112980907A (zh) 2011-11-04 2021-06-18 康奈尔大学 一种用于糖蛋白合成的基于原核生物的无细胞系统
CA3125293A1 (en) 2012-11-07 2014-05-15 Glaxosmithkline Biologicals Sa Production of recombinant vaccine in e. coli by enzymatic conjugation
AU2014206799B9 (en) 2013-01-17 2018-05-10 Janssen Pharmaceuticals, Inc. MDR E. coli specific antibody
CA2907795C (en) * 2013-04-05 2023-09-05 The Governors Of The University Of Alberta Campylobacter vaccine
AR100859A1 (es) 2014-02-24 2016-11-09 Glycovaxyn Ag Polisacáridos y sus usos
CN106794237B (zh) 2014-04-17 2022-04-12 葛兰素史密丝克莱恩生物有限公司 经修饰的宿主细胞及其用途
ES2793023T3 (es) 2014-08-08 2020-11-12 Glaxosmithkline Biologicals Sa Células huésped modificadas y oligosacáridos híbridos para su uso en producción de bioconjugados
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
TWI715617B (zh) 2015-08-24 2021-01-11 比利時商葛蘭素史密斯克藍生物品公司 對抗腸道外病原性大腸桿菌之免疫保護之方法及組合物
GB201610599D0 (en) 2016-06-17 2016-08-03 Glaxosmithkline Biologicals Sa Immunogenic Composition
AR109621A1 (es) 2016-10-24 2018-12-26 Janssen Pharmaceuticals Inc Formulaciones de vacunas contra glucoconjugados de expec
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
GB201704117D0 (en) * 2017-03-15 2017-04-26 London School Hygiene & Tropical Medicine Oligosaccharyl Transferase polypeptide
WO2019035916A1 (en) 2017-08-15 2019-02-21 Northwestern University DESIGN OF PROTEIN GLYCOSYLATION SITES BY RAPID EXPRESSION AND CHARACTERIZATION OF N-GLYCOSYLTRANSFERASES
GB201721576D0 (en) 2017-12-21 2018-02-07 Glaxosmithkline Biologicals Sa Hla antigens and glycoconjugates thereof
GB201721582D0 (en) 2017-12-21 2018-02-07 Glaxosmithkline Biologicals Sa S aureus antigens and immunogenic compositions
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
US11725224B2 (en) 2018-04-16 2023-08-15 Northwestern University Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates
WO2020072127A2 (en) 2018-08-03 2020-04-09 Northwestern University On demand, portable, cell-free molecular sensing platform
US20210395728A1 (en) * 2018-08-29 2021-12-23 Council Of Scientific & Industrial Research Recombinant microbial system for directed evolution of glycocins and method of preparation thereof
EP3894431A2 (en) 2018-12-12 2021-10-20 GlaxoSmithKline Biologicals SA Modified carrier proteins for o-linked glycosylation
CN113646438A (zh) * 2019-01-11 2021-11-12 西北大学 在原核生物细胞裂解物中合成生物缀合物疫苗
PE20212265A1 (es) 2019-03-18 2021-11-30 Janssen Pharmaceuticals Inc Bioconjugados de antigenos-polisacaridos de e. coli, metodos de produccion y metodos de utilizacion de los mismos
MA55365B1 (fr) 2019-03-18 2023-09-27 Janssen Pharmaceuticals Inc Procedes de production de bioconjugues de polysaccharides o-antigenes d'e. coli, leurs compositions et leurs procedes d'utilisation
EP3757217A1 (en) 2019-06-27 2020-12-30 GlaxoSmithKline Biologicals S.A. Methods for protein purification
EP3770269A1 (en) 2019-07-23 2021-01-27 GlaxoSmithKline Biologicals S.A. Quantification of bioconjugate glycosylation
EP3777884A1 (en) 2019-08-15 2021-02-17 GlaxoSmithKline Biologicals S.A. Immunogenic composition
JP2023530154A (ja) 2020-06-18 2023-07-13 グラクソスミスクライン バイオロジカルズ ソシエテ アノニム 赤痢菌4価(Shigella4V)バイオコンジュゲート
BR112022024267A2 (pt) 2020-06-25 2023-01-17 Glaxosmithkline Biologicals Sa Vacina
MX2023003169A (es) 2020-09-17 2023-03-27 Janssen Pharmaceuticals Inc Composiciones de vacunas multivalentes y usos de las mismas.
WO2023118033A1 (en) 2021-12-22 2023-06-29 Glaxosmithkline Biologicals Sa Vaccine
GB202302579D0 (en) 2023-02-23 2023-04-12 Glaxosmithkline Biologicals Sa Immunogenic composition

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0170204B1 (de) 1984-08-01 1991-09-25 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Neue genetische Sequenzen, die durch sie codierten Interferon-Peptide vom Typ I und diese sie produzierende Organismen
US5643758A (en) * 1987-03-10 1997-07-01 New England Biolabs, Inc. Production and purification of a protein fused to a binding protein
JPH09501044A (ja) 1993-05-14 1997-02-04 ジ・アップジョン・カンパニー UDP−GALNAc:ポリペプチド、N−アセチルガラクトサミニルトランスフェラーゼをコードするクローン化されたDNA
US6503744B1 (en) 1999-02-01 2003-01-07 National Research Council Of Canada Campylobacter glycosyltransferases for biosynthesis of gangliosides and ganglioside mimics
JP2003524395A (ja) * 1999-03-02 2003-08-19 ヒューマン ジノーム サイエンシーズ, インコーポレイテッド 細胞内シアリル化経路の操作
EP1285072A2 (en) * 2000-05-12 2003-02-26 Neose Technologies, Inc. In vitro fucosylation recombinant glycopeptides
AU7765801A (en) 2000-06-30 2002-01-08 Flanders Interuniversity Inst Protein glycosylation modification in pichia pastoris
IL163806A0 (en) 2002-03-07 2005-12-18 Eidgenoess Tech Hochschule System and method for the production of recombinant glycosylated proteins in a prokaryotic host
JP4727927B2 (ja) 2002-03-07 2011-07-20 アイトゲネシシェ・テッヒニシェ・ホーホシューレ・チューリッヒ 原核宿主における組換えグリコシル化タンパク質産生のためのシステムおよび方法
BR0312896A (pt) 2002-08-01 2005-06-14 Ca Nat Research Council Glicanos e glicopeptìdeos de campylobacter
US8071113B2 (en) 2004-05-24 2011-12-06 The United States Of America As Represented By The Department Of Health And Human Services Live, oral vaccine for protection against Shigella dysenteriae serotype 1
WO2006119987A2 (en) 2005-05-11 2006-11-16 ETH Zürich Recombinant n-glycosylated proteins from procaryotic cells
HUE039413T2 (hu) 2008-02-20 2018-12-28 Glaxosmithkline Biologicals Sa Prokarióta sejtekbõl származó rekombináns N-glikozilált fehérjékbõl készített biokonjugátumok
LT2501406T (lt) * 2009-11-19 2018-03-26 Glaxosmithkline Biologicals S.A. Biosintetinė sistema sukurianti imunogeninius polisacharidus prokariotinėse gardelėse
AU2011249839B2 (en) * 2010-05-06 2016-05-26 Glaxosmithkline Biologicals S.A. Capsular gram-positive bacteria bioconjugate vaccines

Also Published As

Publication number Publication date
AU2003205502A1 (en) 2003-09-16
US20140335127A1 (en) 2014-11-13
EP1481057A1 (en) 2004-12-01
EP1481057B1 (en) 2006-02-15
AU2003205502C1 (en) 2011-08-25
AU2003205502B2 (en) 2008-01-31
DE60303591T2 (de) 2007-03-01
IL163806A (en) 2012-08-30
ES2258214T3 (es) 2006-08-16
WO2003074687A1 (en) 2003-09-12
DE60303591D1 (de) 2006-04-20
US20050287628A1 (en) 2005-12-29
IL220322A0 (en) 2012-07-31
US8703471B2 (en) 2014-04-22
US20090074798A1 (en) 2009-03-19
PT1481057E (pt) 2006-05-31
IL163806A0 (en) 2005-12-18
KR20090110951A (ko) 2009-10-23
CA2477794A1 (en) 2003-09-12
CA2477794C (en) 2013-08-20
ATE317897T1 (de) 2006-03-15

Similar Documents

Publication Publication Date Title
DK1481057T3 (da) System og fremgangsmåde til fremstilling af rekombinante glycosylerede proteiner i en prokaryot vært
MX2008011669A (es) Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina.
RU2473690C2 (ru) Генетически кодируемые флюоресцентные кумариновые аминокислоты
DK1369486T3 (da) Plasmid-shuttlevektor mellem Escherichia coli og Brevibacillus
BRPI0207394B8 (pt) dna codificando uma proteína de fusão proteína codificada por um dna, plasmídeo, micro-organismo hospedeiro, processo para a produção fermentativa de uma proteína de fusão e processo para a produçao de insulina
EP1179059A4 (en) ISOLATION OF BIOLOGICAL MODULATORS FROM GENE FRAGMENT BANKS WITH BIOLOGICAL DIVERSITY
ATE545705T1 (de) Neues induzierbares genexpressionssystem auf ecdysonrezeptor/invertebraten-retinoid-x-rezept r-basis
DK0737747T3 (da) Cytoplasmatisk ekspression af antistoffer, antistoffragmenter og antistoffragmentfusionsmolekyler I E. coli
NO20075088L (no) Vertscelle omfattende en vektor for fremstilling av proteiner som krever gamma-karboksylering
IL173474A0 (en) Methods for producing recombinant proteins
Luxmi et al. Proteases shape the Chlamydomonas secretome: comparison to classical neuropeptide processing machinery
WO2003077852A3 (en) CARTILAGE REGENERATION USING CHONDROCYTE AND TGF-β
WO2002052029A9 (fr) Methode permettant de produire une proteine de recombinaison et une proteine fusionnee
DE3785798D1 (de) Verfahren zur gentechnologischengewinnung von proteinen unter verwendung gramnegativer wirtzellen.
BR0214542A (pt) Célula hospedeira, sistema de expressão, e, processos de preparação de uma célula hospedeira, e de uma proteìna alvo
Wrede et al. Peptide design aided by neural networks: biological activity of artificial signal peptidase I cleavage sites
CN1369014A (zh) 蛋白质生产
DK1442134T3 (da) Fremgangsmåde til fremstilling og identifikation af oplöselige proteindomæner
ATE371732T1 (de) Verfahren zur genexpression
WO2004034780A3 (es) Método para la producción de proteínas recombinantes en la glándula mamaria de mamíferos no transgénicos
AU4917300A (en) Method for producing a recombinant protein
DE60238333D1 (de) Auf antibiotikum basierendes genregulationssystem
DK1713930T3 (da) Hurtig screeningsmetode for translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionspartnere screenet derfra
EP1576096A4 (en) N-ACETYLGLUCOSAMINYLTRANSFERASE VB CODING SEQUENCE, RECOMBINANT CELLS AND METHOD
AU6252294A (en) A mutant protein and methods and materials for making and using it