IL163806A0 - System and method for the production of recombinant glycosylated proteins in a prokaryotic host - Google Patents
System and method for the production of recombinant glycosylated proteins in a prokaryotic hostInfo
- Publication number
- IL163806A0 IL163806A0 IL16380603A IL16380603A IL163806A0 IL 163806 A0 IL163806 A0 IL 163806A0 IL 16380603 A IL16380603 A IL 16380603A IL 16380603 A IL16380603 A IL 16380603A IL 163806 A0 IL163806 A0 IL 163806A0
- Authority
- IL
- Israel
- Prior art keywords
- production
- recombinant
- prokaryotic host
- glycosylated proteins
- oligosaccharide
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
- A61K47/549—Sugars, nucleosides, nucleotides or nucleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
- C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
- C07K16/121—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Helicobacter (Campylobacter) (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH3942002 | 2002-03-07 | ||
| US36465502P | 2002-03-14 | 2002-03-14 | |
| PCT/CH2003/000153 WO2003074687A1 (en) | 2002-03-07 | 2003-03-05 | System and method for the production of recombinant glycosylated proteins in a prokaryotic host |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL163806A0 true IL163806A0 (en) | 2005-12-18 |
Family
ID=27789602
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL16380603A IL163806A0 (en) | 2002-03-07 | 2003-03-05 | System and method for the production of recombinant glycosylated proteins in a prokaryotic host |
| IL163806A IL163806A (en) | 2002-03-07 | 2004-08-30 | System and method for the production of recombinant glycosylated proteins in a prokaryotic host |
| IL220322A IL220322A0 (en) | 2002-03-07 | 2012-06-12 | System and method for the production of recombinant glycosylated proteins in a prokaryotic host |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL163806A IL163806A (en) | 2002-03-07 | 2004-08-30 | System and method for the production of recombinant glycosylated proteins in a prokaryotic host |
| IL220322A IL220322A0 (en) | 2002-03-07 | 2012-06-12 | System and method for the production of recombinant glycosylated proteins in a prokaryotic host |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US20050287628A1 (xx) |
| EP (1) | EP1481057B1 (xx) |
| KR (1) | KR20090110951A (xx) |
| AT (1) | ATE317897T1 (xx) |
| AU (1) | AU2003205502C1 (xx) |
| CA (1) | CA2477794C (xx) |
| DE (1) | DE60303591T2 (xx) |
| DK (1) | DK1481057T3 (xx) |
| ES (1) | ES2258214T3 (xx) |
| IL (3) | IL163806A0 (xx) |
| PT (1) | PT1481057E (xx) |
| WO (1) | WO2003074687A1 (xx) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL163806A0 (en) * | 2002-03-07 | 2005-12-18 | Eidgenoess Tech Hochschule | System and method for the production of recombinant glycosylated proteins in a prokaryotic host |
| AU2011218682B2 (en) * | 2005-05-11 | 2013-11-21 | Eth Zurich | Recombinant N-glycosylated proteins from procaryotic cells |
| WO2006119987A2 (en) * | 2005-05-11 | 2006-11-16 | ETH Zürich | Recombinant n-glycosylated proteins from procaryotic cells |
| JP5667880B2 (ja) | 2008-01-03 | 2015-02-12 | コーネル リサーチ ファンデーション インコーポレイテッド | 原核生物におけるグリコシル化されたタンパク質の発現方法 |
| HUE039413T2 (hu) | 2008-02-20 | 2018-12-28 | Glaxosmithkline Biologicals Sa | Prokarióta sejtekbõl származó rekombináns N-glikozilált fehérjékbõl készített biokonjugátumok |
| LT2501406T (lt) | 2009-11-19 | 2018-03-26 | Glaxosmithkline Biologicals S.A. | Biosintetinė sistema sukurianti imunogeninius polisacharidus prokariotinėse gardelėse |
| AU2011249839B2 (en) | 2010-05-06 | 2016-05-26 | Glaxosmithkline Biologicals S.A. | Capsular gram-positive bacteria bioconjugate vaccines |
| AU2012297533B2 (en) | 2011-08-12 | 2016-02-25 | Instituto De Investigaciones Biotecnologicas-Instituto Tecnologico De Chascomus Consejo De Investigaciones Cientificas Y Tecnicas, Universidad Nacional De San Martin | Method of diagnosing bacterial infections using bacterial glycoproteins |
| EP2753353A1 (en) | 2011-09-06 | 2014-07-16 | Glycovaxyn AG | Bioconjugate vaccines made in prokaryotic cells |
| CN112980907A (zh) | 2011-11-04 | 2021-06-18 | 康奈尔大学 | 一种用于糖蛋白合成的基于原核生物的无细胞系统 |
| CA3125293A1 (en) | 2012-11-07 | 2014-05-15 | Glaxosmithkline Biologicals Sa | Production of recombinant vaccine in e. coli by enzymatic conjugation |
| AU2014206799B9 (en) | 2013-01-17 | 2018-05-10 | Janssen Pharmaceuticals, Inc. | MDR E. coli specific antibody |
| CA2907795C (en) * | 2013-04-05 | 2023-09-05 | The Governors Of The University Of Alberta | Campylobacter vaccine |
| AR100859A1 (es) | 2014-02-24 | 2016-11-09 | Glycovaxyn Ag | Polisacáridos y sus usos |
| CN106794237B (zh) | 2014-04-17 | 2022-04-12 | 葛兰素史密丝克莱恩生物有限公司 | 经修饰的宿主细胞及其用途 |
| ES2793023T3 (es) | 2014-08-08 | 2020-11-12 | Glaxosmithkline Biologicals Sa | Células huésped modificadas y oligosacáridos híbridos para su uso en producción de bioconjugados |
| US9616114B1 (en) | 2014-09-18 | 2017-04-11 | David Gordon Bermudes | Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity |
| TWI715617B (zh) | 2015-08-24 | 2021-01-11 | 比利時商葛蘭素史密斯克藍生物品公司 | 對抗腸道外病原性大腸桿菌之免疫保護之方法及組合物 |
| GB201610599D0 (en) | 2016-06-17 | 2016-08-03 | Glaxosmithkline Biologicals Sa | Immunogenic Composition |
| AR109621A1 (es) | 2016-10-24 | 2018-12-26 | Janssen Pharmaceuticals Inc | Formulaciones de vacunas contra glucoconjugados de expec |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
| GB201704117D0 (en) * | 2017-03-15 | 2017-04-26 | London School Hygiene & Tropical Medicine | Oligosaccharyl Transferase polypeptide |
| WO2019035916A1 (en) | 2017-08-15 | 2019-02-21 | Northwestern University | DESIGN OF PROTEIN GLYCOSYLATION SITES BY RAPID EXPRESSION AND CHARACTERIZATION OF N-GLYCOSYLTRANSFERASES |
| GB201721576D0 (en) | 2017-12-21 | 2018-02-07 | Glaxosmithkline Biologicals Sa | Hla antigens and glycoconjugates thereof |
| GB201721582D0 (en) | 2017-12-21 | 2018-02-07 | Glaxosmithkline Biologicals Sa | S aureus antigens and immunogenic compositions |
| US11530432B2 (en) | 2018-03-19 | 2022-12-20 | Northwestern University | Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates |
| US11725224B2 (en) | 2018-04-16 | 2023-08-15 | Northwestern University | Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates |
| WO2020072127A2 (en) | 2018-08-03 | 2020-04-09 | Northwestern University | On demand, portable, cell-free molecular sensing platform |
| US20210395728A1 (en) * | 2018-08-29 | 2021-12-23 | Council Of Scientific & Industrial Research | Recombinant microbial system for directed evolution of glycocins and method of preparation thereof |
| EP3894431A2 (en) | 2018-12-12 | 2021-10-20 | GlaxoSmithKline Biologicals SA | Modified carrier proteins for o-linked glycosylation |
| CN113646438A (zh) * | 2019-01-11 | 2021-11-12 | 西北大学 | 在原核生物细胞裂解物中合成生物缀合物疫苗 |
| PE20212265A1 (es) | 2019-03-18 | 2021-11-30 | Janssen Pharmaceuticals Inc | Bioconjugados de antigenos-polisacaridos de e. coli, metodos de produccion y metodos de utilizacion de los mismos |
| MA55365B1 (fr) | 2019-03-18 | 2023-09-27 | Janssen Pharmaceuticals Inc | Procedes de production de bioconjugues de polysaccharides o-antigenes d'e. coli, leurs compositions et leurs procedes d'utilisation |
| EP3757217A1 (en) | 2019-06-27 | 2020-12-30 | GlaxoSmithKline Biologicals S.A. | Methods for protein purification |
| EP3770269A1 (en) | 2019-07-23 | 2021-01-27 | GlaxoSmithKline Biologicals S.A. | Quantification of bioconjugate glycosylation |
| EP3777884A1 (en) | 2019-08-15 | 2021-02-17 | GlaxoSmithKline Biologicals S.A. | Immunogenic composition |
| JP2023530154A (ja) | 2020-06-18 | 2023-07-13 | グラクソスミスクライン バイオロジカルズ ソシエテ アノニム | 赤痢菌４価（Ｓｈｉｇｅｌｌａ４Ｖ）バイオコンジュゲート |
| BR112022024267A2 (pt) | 2020-06-25 | 2023-01-17 | Glaxosmithkline Biologicals Sa | Vacina |
| MX2023003169A (es) | 2020-09-17 | 2023-03-27 | Janssen Pharmaceuticals Inc | Composiciones de vacunas multivalentes y usos de las mismas. |
| WO2023118033A1 (en) | 2021-12-22 | 2023-06-29 | Glaxosmithkline Biologicals Sa | Vaccine |
| GB202302579D0 (en) | 2023-02-23 | 2023-04-12 | Glaxosmithkline Biologicals Sa | Immunogenic composition |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0170204B1 (de) | 1984-08-01 | 1991-09-25 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Neue genetische Sequenzen, die durch sie codierten Interferon-Peptide vom Typ I und diese sie produzierende Organismen |
| US5643758A (en) * | 1987-03-10 | 1997-07-01 | New England Biolabs, Inc. | Production and purification of a protein fused to a binding protein |
| JPH09501044A (ja) | 1993-05-14 | 1997-02-04 | ジ・アップジョン・カンパニー | ＵＤＰ−ＧＡＬＮＡｃ：ポリペプチド、Ｎ−アセチルガラクトサミニルトランスフェラーゼをコードするクローン化されたＤＮＡ |
| US6503744B1 (en) | 1999-02-01 | 2003-01-07 | National Research Council Of Canada | Campylobacter glycosyltransferases for biosynthesis of gangliosides and ganglioside mimics |
| JP2003524395A (ja) * | 1999-03-02 | 2003-08-19 | ヒューマン ジノーム サイエンシーズ， インコーポレイテッド | 細胞内シアリル化経路の操作 |
| EP1285072A2 (en) * | 2000-05-12 | 2003-02-26 | Neose Technologies, Inc. | In vitro fucosylation recombinant glycopeptides |
| AU7765801A (en) | 2000-06-30 | 2002-01-08 | Flanders Interuniversity Inst | Protein glycosylation modification in pichia pastoris |
| IL163806A0 (en) | 2002-03-07 | 2005-12-18 | Eidgenoess Tech Hochschule | System and method for the production of recombinant glycosylated proteins in a prokaryotic host |
| JP4727927B2 (ja) | 2002-03-07 | 2011-07-20 | アイトゲネシシェ・テッヒニシェ・ホーホシューレ・チューリッヒ | 原核宿主における組換えグリコシル化タンパク質産生のためのシステムおよび方法 |
| BR0312896A (pt) | 2002-08-01 | 2005-06-14 | Ca Nat Research Council | Glicanos e glicopeptìdeos de campylobacter |
| US8071113B2 (en) | 2004-05-24 | 2011-12-06 | The United States Of America As Represented By The Department Of Health And Human Services | Live, oral vaccine for protection against Shigella dysenteriae serotype 1 |
| WO2006119987A2 (en) | 2005-05-11 | 2006-11-16 | ETH Zürich | Recombinant n-glycosylated proteins from procaryotic cells |
| HUE039413T2 (hu) | 2008-02-20 | 2018-12-28 | Glaxosmithkline Biologicals Sa | Prokarióta sejtekbõl származó rekombináns N-glikozilált fehérjékbõl készített biokonjugátumok |
| LT2501406T (lt) * | 2009-11-19 | 2018-03-26 | Glaxosmithkline Biologicals S.A. | Biosintetinė sistema sukurianti imunogeninius polisacharidus prokariotinėse gardelėse |
| AU2011249839B2 (en) * | 2010-05-06 | 2016-05-26 | Glaxosmithkline Biologicals S.A. | Capsular gram-positive bacteria bioconjugate vaccines |
-
2003
- 2003-03-05 IL IL16380603A patent/IL163806A0/xx unknown
- 2003-03-05 DE DE60303591T patent/DE60303591T2/de not_active Expired - Lifetime
- 2003-03-05 CA CA2477794A patent/CA2477794C/en not_active Expired - Lifetime
- 2003-03-05 AU AU2003205502A patent/AU2003205502C1/en not_active Expired
- 2003-03-05 AT AT03702276T patent/ATE317897T1/de active
- 2003-03-05 US US10/506,917 patent/US20050287628A1/en not_active Abandoned
- 2003-03-05 KR KR1020097020064A patent/KR20090110951A/ko not_active Application Discontinuation
- 2003-03-05 ES ES03702276T patent/ES2258214T3/es not_active Expired - Lifetime
- 2003-03-05 EP EP03702276A patent/EP1481057B1/en not_active Expired - Lifetime
- 2003-03-05 WO PCT/CH2003/000153 patent/WO2003074687A1/en active Search and Examination
- 2003-03-05 PT PT03702276T patent/PT1481057E/pt unknown
- 2003-03-05 DK DK03702276T patent/DK1481057T3/da active
-
2004
- 2004-08-30 IL IL163806A patent/IL163806A/en active IP Right Grant
-
2008
- 2008-07-21 US US12/219,383 patent/US8703471B2/en not_active Expired - Lifetime
-
2012
- 2012-06-12 IL IL220322A patent/IL220322A0/en active IP Right Grant
-
2014
- 2014-02-25 US US14/189,673 patent/US20140335127A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003205502A1 (en) | 2003-09-16 |
| US20140335127A1 (en) | 2014-11-13 |
| EP1481057A1 (en) | 2004-12-01 |
| EP1481057B1 (en) | 2006-02-15 |
| AU2003205502C1 (en) | 2011-08-25 |
| AU2003205502B2 (en) | 2008-01-31 |
| DE60303591T2 (de) | 2007-03-01 |
| IL163806A (en) | 2012-08-30 |
| ES2258214T3 (es) | 2006-08-16 |
| WO2003074687A1 (en) | 2003-09-12 |
| DE60303591D1 (de) | 2006-04-20 |
| US20050287628A1 (en) | 2005-12-29 |
| DK1481057T3 (da) | 2006-05-15 |
| IL220322A0 (en) | 2012-07-31 |
| US8703471B2 (en) | 2014-04-22 |
| US20090074798A1 (en) | 2009-03-19 |
| PT1481057E (pt) | 2006-05-31 |
| KR20090110951A (ko) | 2009-10-23 |
| CA2477794A1 (en) | 2003-09-12 |
| CA2477794C (en) | 2013-08-20 |
| ATE317897T1 (de) | 2006-03-15 |
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