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CO5580846A2 - Aptameros que contienen secuencias de acidos nucleicos o analogos de acidos nucleicos unidos homologamente, o en complejos nuevos - Google Patents

Aptameros que contienen secuencias de acidos nucleicos o analogos de acidos nucleicos unidos homologamente, o en complejos nuevos

Info

Publication number
CO5580846A2
CO5580846A2 CO04037600A CO04037600A CO5580846A2 CO 5580846 A2 CO5580846 A2 CO 5580846A2 CO 04037600 A CO04037600 A CO 04037600A CO 04037600 A CO04037600 A CO 04037600A CO 5580846 A2 CO5580846 A2 CO 5580846A2
Authority
CO
Colombia
Prior art keywords
interaction
nucleic acids
sequences
parallel
joined together
Prior art date
Application number
CO04037600A
Other languages
English (en)
Inventor
Glen H Erikson
Jasmine I Daksis
Original Assignee
Ingeneus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ingeneus Corp filed Critical Ingeneus Corp
Publication of CO5580846A2 publication Critical patent/CO5580846A2/es

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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/13—Decoys
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/15—Nucleic acids forming more than 2 strands, e.g. TFOs
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/15—Nucleic acids forming more than 2 strands, e.g. TFOs
    • C12N2310/151—Nucleic acids forming more than 2 strands, e.g. TFOs more than 3 strands, e.g. tetrads, H-DNA
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
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    • C12Q2527/00—Reactions demanding special reaction conditions
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/119—Triple helix formation
    • C—CHEMISTRY; METALLURGY
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/137—Metal/ion, e.g. metal label
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/173—Nucleic acid detection characterized by the use of physical, structural and functional properties staining/intercalating agent, e.g. ethidium bromide

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  • Communicable Diseases (AREA)
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  • General Physics & Mathematics (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1.- Un método para enlazar un ligando, el método caracterizado porque comprende:proporcionar un aptámero que comprende por lo menos dos secuencias que contienen nucleobases heteropoliméricas paralelas o antiparalelas unidas conjuntamente por la interacción de bases complementarias de Watson-Crick o por la interacción de bases homólogas, en donde: (a) el aptámero comprende un dúplex, y las dos por lo menos dos secuencias están unidas conjuntamente por la interacción de bases complementarias de Waton-Crick con direccionalidad paralela; (b) el aptámero comprende un dúplex, y las por lo menos dos secuencias están unidas conjuntamente por la interacción de bases homólogas con la direccionalidad paralela o antiparalela; (c) el aptámero comprende un tríplex y las por lo menos dos secuencias están unidas conjuntamente por la interacción de bases complementarias de Watson-Crick con direccionalidad paralela o antiparalela; (d) el aptámero comprende un tríplex, y las por lo menos dos secuencias están unidas conjuntamente por la interacción de bases homólogas con direccionalidad paralela o antiparalela, (c) el aptámero comprende un cuadrúplex y las por lo menos dos secuencias están unidas conjuntamente por la interacción de bases complementarias de Watson-Crick con direccionalidad paralela o antiparalela; o (f) el aptámero comprende un cuadrúplex, y las por lo menos dos secuencias están unidas conjuntamente por la interacción de bases homólogas con direccionalidad paralela o antiparalela, yponer en contacto el aptámero con el ligando para enlazar el ligando al aptámero mediante la interacción diferente por el emparejamiento de bases de Watson-Crick de nucleobase a nucleobase.
CO04037600A 2001-09-24 2004-04-23 Aptameros que contienen secuencias de acidos nucleicos o analogos de acidos nucleicos unidos homologamente, o en complejos nuevos CO5580846A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/961,865 US6858390B2 (en) 1998-12-31 2001-09-24 Aptamers containing sequences of nucleic acid or nucleic acid analogues bound homologously, or in novel complexes

Publications (1)

Publication Number Publication Date
CO5580846A2 true CO5580846A2 (es) 2005-11-30

Family

ID=25505117

Family Applications (1)

Application Number Title Priority Date Filing Date
CO04037600A CO5580846A2 (es) 2001-09-24 2004-04-23 Aptameros que contienen secuencias de acidos nucleicos o analogos de acidos nucleicos unidos homologamente, o en complejos nuevos

Country Status (13)

Country Link
US (1) US6858390B2 (es)
EP (1) EP1434882A4 (es)
JP (1) JP2005503175A (es)
KR (1) KR20040035878A (es)
CN (1) CN1578840A (es)
AU (1) AU2002330708B2 (es)
CA (1) CA2461526A1 (es)
CO (1) CO5580846A2 (es)
IL (1) IL161030A0 (es)
MX (1) MXPA04002766A (es)
RU (1) RU2004112533A (es)
WO (1) WO2003027319A1 (es)
ZA (1) ZA200402395B (es)

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AU2002330708B2 (en) 2005-08-25
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US6858390B2 (en) 2005-02-22
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AU2002330708A1 (en) 2003-04-07
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WO2003027319A1 (en) 2003-04-03
MXPA04002766A (es) 2005-04-08
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