CN216137429U - Device for extracting platelet-rich plasma from blood - Google Patents

Abstract

The present invention relates to a device for extracting platelet rich plasma from blood. The device includes: a first syringe; the first injector is detachably connected to the communicating piece in a conical head-down posture; a collection container removably connected to the communication member; a second syringe removably connected to the communication member. The second injector containing platelet-rich plasma is detachably connected to the spraying device after being detached from the communicating piece; and the third injector contains the coagulant and is detachably connected with the spraying device so as to ensure that the second injector, the third injector and the spraying device are communicated in a fluid mode, and the platelet-rich plasma in the second injector and the coagulant in the third injector can be transferred to the spraying device and are mixed in the spraying device to generate platelet-rich gel to be sprayed out.

Description

Device for extracting platelet-rich plasma from blood

Technical Field

The utility model relates to the technical field of medical instruments, in particular to a device for extracting platelet-rich plasma from blood.

Background

After centrifugation, the blood is divided into three layers, namely a Platelet-Poor Plasma (PPP) layer, a Platelet-Rich Plasma (PRP) layer and a red blood cell layer from top to bottom. Liquid PRP is generally injected to a treatment site to accelerate wound healing and repair cartilage damage, etc., and gel-like PRP is suitable for adhering, filling and covering wounds and preventing loss of growth factors, and thus is widely used in the medical field. The PRP is usually extracted by primary centrifugation or secondary centrifugation, but the primary centrifugation is usually performed by extracting the intermediate PRP layer, which causes the PRP in the intermediate layer to diffuse into the upper PPP layer or the lower erythrocyte layer, resulting in low PRP extraction concentration and recovery rate. The second centrifugation method requires two centrifugation processes, which obviously makes the required extraction process more complicated and time-consuming. In addition, the liquid PRP obtained by extraction is generally directly injected in the prior art, so the using effect is more general.

Therefore, there is a need in the art for an extraction device that provides high extraction concentration and recovery, a simple and time-consuming extraction process, and good results.

SUMMERY OF THE UTILITY MODEL

The present invention aims to provide a device for extracting platelet rich plasma from blood that solves at least some of the problems described above.

According to one aspect of the present invention, there is provided a device for extracting platelet rich plasma from blood, the device comprising: a first syringe which can contain anticoagulated blood and can centrifuge the anticoagulated blood for 5 to 45 minutes at a relative centrifugal force of 300 to 3000g in a posture that a conical head faces downwards so that contents in the first syringe are layered into a platelet poor plasma layer, a platelet rich plasma layer and a red blood cell layer from top to bottom; the first injector is detachably connected to the communicating piece in a conical head-down posture; a collection container removably connected to the communication member such that the first syringe and the collection container are in communication via the communication member and the layer of red blood cells in the first syringe can be transferred into the collection container via the communication member; a second syringe detachably connected to the communication member so that the platelet rich plasma layer in the first syringe can be transferred into the second syringe through the communication member, and the platelet rich plasma layer is only contained in the second syringe to extract platelet rich plasma; the second syringe containing platelet-rich plasma is detachably connected to the spraying device after being detached from the communicating piece; and a third syringe which can contain a coagulant and is detachably connected with the spraying device so as to enable the second syringe, the third syringe and the spraying device to be communicated in a fluid mode, wherein the platelet-rich plasma in the second syringe and the coagulant in the third syringe can be transferred to the spraying device, and are mixed in the spraying device to generate platelet-rich gel which is then sprayed out.

Compared with the prior art, the utility model can ensure the clear layering of the blood in the first injector after centrifugal movement and avoid damaging the red blood cells by setting the relative centrifugal force and the centrifugal time range of the first injector for centrifugal movement. In addition, through assembling first syringe, UNICOM's piece, collection container and second syringe, only need carry out once centrifugal operation and can realize PRP's extraction, it is short and simple structure consuming time. The PRP can be transferred into the second injector by operating the first injector, which enables the bulk transfer of the PRP layer while reducing interlayer disturbances, thereby improving PRP recovery. In addition, by operating the advancing degree of the first plunger rod, a desired PRP concentration can be obtained as required, and the applicability is high. Finally, by connecting the second syringe and the third syringe through the spraying device, the PRP gel can be prepared, and the using effect of the prepared PRP is further improved.

Preferably, the conical heads of the first syringe, the collection container, the second syringe and the third syringe are all detachably connected with sheaths.

Preferably, the communication member is a two-way valve or a three-way valve.

Preferably, the spray coating device comprises: a holding frame holding the second syringe and the third syringe juxtaposed to each other therein; a push plate mounted to the heads of the second syringe and the third syringe core rod; the spray tee joint comprises two liquid inlets and a liquid outlet, and the two liquid inlets of the spray tee joint are respectively connected to the conical heads of the second injector and the third injector; a nozzle connected to the liquid outlet of the spray tee to communicate the second and third syringes and the nozzle, wherein the platelet rich in the second syringe and the coagulant in the third syringe are transferred into the nozzle to mix to produce a gel in the nozzle; and the spray head is arranged in the nozzle to spray the atomized gel.

Preferably, the conical heads of the first, second and third syringes are removably attachable with a sheath.

Preferably, the first syringe is connected to the communication via a sealed connection.

Preferably, the sealing connection is configured as a luer fitting.

Additional features and advantages of the utility model will be set forth in part in the description which follows, and in part will be apparent to those having ordinary skill in the art upon examination of the following, or may be learned from the practice of the utility model.

Drawings

Embodiments of the utility model are described in detail below with reference to the attached drawing figures, wherein:

FIG. 1 is a first schematic view of an apparatus for extracting platelet rich plasma from blood according to the present invention;

FIG. 2 is a second schematic view of an apparatus for extracting platelet rich plasma from blood according to the present invention;

fig. 3 is a schematic flow diagram of an apparatus for extracting platelet rich plasma from blood according to the present invention.

Description of reference numerals:

10-a first syringe; 11-a communication member; 12-a collection vessel; 13-a second syringe; 14-a third syringe; 15-a spraying device; 151-a support frame; 152-push plate.

Detailed Description

Referring now to the drawings, an illustrative embodiment of the disclosed device for extracting platelet rich plasma from blood will be described in detail. Although the drawings are provided to present some embodiments of the utility model, the drawings are not necessarily to scale of particular embodiments, and certain features may be exaggerated, removed, or partially sectioned to better illustrate and explain the present disclosure. The position of some components in the drawings can be adjusted according to actual requirements on the premise of not influencing the technical effect. The appearances of the phrase "in the drawings" or similar language in the specification are not necessarily referring to all drawings or examples.

Certain directional terms used hereinafter to describe the drawings, such as "inner", "outer", "above", "below", and other directional terms, will be understood to have their normal meaning and refer to those directions as they normally relate to when viewing the drawings. Unless otherwise indicated, the directional terms described herein are generally in accordance with conventional directions as understood by those skilled in the art.

The terms "first," "second," and the like, as used herein, do not denote any order, quantity, or importance, but rather are used to distinguish one element from another.

As used herein, the terms "joined," "connected," and the like, are intended to encompass both components which are indirectly joined together through intervening layers (e.g., adhesives, welds, etc.) or intermediate members (e.g., connectors, transitions, etc.), and components which are directly joined together without any intervening layers (e.g., adhesives, welds, etc.) or intermediate members (e.g., connectors, transitions, etc.).

Fig. 1 and 2 show a platelet-rich plasma extraction device of the present invention, which is simpler than the prior art extraction device, and can ensure the concentration of extracted PRP and improve the PRP usage effect while simplifying the extraction complexity and reducing the extraction time. The device may include, among other things, a first syringe 10, a communication 11, a collection container 12, a second syringe 13, a spray device 15, and a third syringe 14.

Specifically, the first syringe 10 includes a barrel, a core rod located on the inner wall of the barrel and capable of sliding along the inner wall of the barrel in a sealing manner, and a conical head located at one end of the barrel. Alternatively, the first syringe 10, the second syringe 13 and the third syringe 14 may also be medical devices having similar functions in the art. The communication member 11 may be a two-way valve or a three-way valve.

In case the communication member 11 is designed as a two-way valve, the two-way valve may comprise a two-way pipe, a two-way valve spool located in the two-way pipe and a rotary piston which may control the rotation of the two-way valve spool. The rotating piston is rotated to enable the channel in the two-way valve core to align with the two ends of the two-way pipe or enable the two-way valve core to block the two-way pipe, so that containers connected to the two ends of the two-way pipe are communicated or disconnected. Wherein the first syringe 10 and the collection container 12 may be detachably connected to both ends of a two-way valve, respectively, such that the first syringe 10 and the collection container 12 are connected or disconnected via the two-way valve, so that the contents in the first syringe 10 may be transferred into the collection container 12 or prevented from being transferred therebetween via the two-way valve by pushing the first syringe 10 core rod. In practice, the collection container 12 may be a syringe or a waste container. In the case where the collection container 12 is detached from the two-way valve, the second syringe 13 may be connected to the two-way valve instead of the collection container 12 to be connected to the first syringe 10 via the two-way valve, so that the contents in the first syringe 10 may be transferred into the second syringe 13 via the two-way valve by pushing on the first syringe 10 plunger. Alternatively, in the case where both the first syringe 10 and the collection container 12 are detached from the two-way valve, the first syringe 10 and the second syringe 13 may be respectively connected to both ends of a new unused two-way valve to communicate the first syringe 10 and the second syringe 13 via the new unused two-way valve, so that the transferred contents are not mixed with the remaining substance in the two-way valve during the transfer of the contents of the first syringe 10 into the second syringe 13.

In case the communication member 11 is designed as a three-way valve, the three-way valve may be configured to include a three-way pipe, a three-way spool located within the three-way pipe, and a rotary piston that may control the rotation of the three-way spool. The channel in the three-way valve core can be communicated with any two ports of the three-way pipe by rotating the rotary piston, so that any two containers connected to the three ports of the three-way pipe are communicated and disconnected with the other container. Wherein, the first syringe 10, the collection container 12 and the second syringe 13 can be respectively connected to three ends of a three-way valve, so that the rotary piston of the three-way valve is adjusted as required to enable the first syringe 10 to be communicated with the collection container 12 but not communicated with the second syringe 13, or the first syringe 10 is adjusted to be not communicated with the collection container 12 but communicated with the second syringe 13, and then the content in the first syringe 10 can be transferred into the collection container 12 or the second syringe 13 as required.

Alternatively, one end of the three-way valve may be designed as a vent end, and the first syringe 10 and the collection container 12 may be connected to the other two ends of the three-way valve, and the contents of the first syringe 10 may be transferred into the collection container 12 via the three-way valve by turning the rotary piston to close the vent end. The collection container 12 is then either three-way disconnected and the second syringe 13 is connected to the three-way valve instead of the collection container 12, or both the first syringe 10 and the collection container 12 are disconnected from the three-way valve and the first syringe 10 and the second syringe 13 are connected by a new unused three-way valve, so that the first syringe 10 and the third syringe 14 are connected via the three-way valve. The contents of the first syringe 10 are transferred into the second syringe 13 through the three-way valve by rotating the piston to close the vent end. Finally, the vented end is communicated to the second syringe 13 by rotating the plunger, pulling the second syringe 13 plunger stem to draw the remaining contents of the three-way valve stem into the second syringe 13. Optionally, the vent end of the three-way valve may be provided with a sterilizing filter membrane.

The second syringe 13 may be detachably connected to the spraying device 15 after being detached from the communicating member 11. The third injector 14 may be detachably connected to the spraying device 15, so that both the second injector 13 and the third injector 14 can be connected to the spraying device 15, and the contents of the second injector 13 and the third injector 14 are transferred into the spraying device 15 by pushing the core rods of the second injector 13 and the third injector 14, and are mixed in the spraying device 15 and then are sprayed out through the spraying device 15.

Alternatively, the spray coating device 15 may include a support frame 151, a thrust plate 152, a spray tee, a nozzle, and a spray head. The second syringe 13 and the third syringe 14 may be held in parallel on the holding frame 151. The spray tee may comprise two liquid inlets and one liquid outlet, wherein the two liquid inlets are respectively connected to the conical heads of the second injector 13 and the third injector 14, and the liquid outlet is connected to the nozzle. Thus, the contents of the second and third injectors 13, 14 can be transferred through the spray tee into the nozzle and mixed within the nozzle by pushing the push plate 152. The nozzle is also internally provided with a spray head so as to atomize and spray the mixture in the nozzle.

Optionally, the conical head of the first syringe 10 may be detachably connected with a sheath when detached from the communicating member 11, so as to prevent the contents of the first syringe 10 from being contaminated by the external environment. Similarly, the second syringe 13 may be detachably connected with a sheath when the communication member 11 and the spraying device 15 are detached, and the third syringe 14 may be detachably connected with a sheath when the spraying device 15 is detached.

Alternatively, the first syringe 10 may be connected to the communicating member 11 by a detachable sealing connection such as a luer fitting. Similarly, the second syringe 13 may be connected to the communication member 11 and the spraying device 15 by a detachable sealing connection, and the third syringe 14 may be connected to the spraying device 15 by a detachable sealing connection. In addition, the conical head of the first syringe 10 may also have a needle assembly removably attached thereto.

Alternatively, the first syringe 10, the communication member 11, the collection container 12, the second syringe 13 and the spraying device 15 may all be made of a transparent material such as plastic or glass, and the transfer of the substance from the first syringe 10 to the collection container 12 through the communication member 11, the transfer of the substance from the first syringe 10 to the second syringe 13 through the communication member 11, and the transfer of the substance from the second syringe 13 and the third syringe 14 to the spraying device 15 can all be clearly observed by the operator, so that the use of the extraction device of the present invention is convenient.

The process of PRP extraction and gel preparation using the extraction device of the present invention is schematically illustrated below with reference to fig. 3. It is to be noted that only the communication 11 will be described below for the extraction device by way of example as a three-way valve, but that the communication 11 or other examples of the above-mentioned components can also be incorporated in the device below.

From the present viewpoint, it is generally considered that the recovery rate of PRP extracted by the secondary centrifugation technique is higher and PRP with a higher platelet concentration can be prepared than by the primary centrifugation technique. However, the inventors believe that the essence of extracting PRP is: sufficient centrifugation to ensure that cells are packed in a density gradient and tend to be constant, and disturbance reduction in separation of the PRP layer, therefore, the factor influencing the extraction of PRP is not to use a so-called primary centrifugation technique or a secondary centrifugation technique, but to influence the stable packing process of the centrifuged cells and whether disturbance is caused in collection of the corresponding components. Thus, the extraction efficiency of PRP by the secondary centrifugation technique is certainly better than that by the primary centrifugation technique. During the centrifugation process, the cell accumulation tends to be stable by adjusting the relative centrifugal force and the centrifugation time. In the PRP separation process, the reduced perturbation of the intermediate PRP layer prevents the platelets from spreading to the adjacent upper and lower layers, thereby collecting PRP with higher platelet recovery.

First, a predetermined amount of anticoagulant is drawn using the first syringe 10, and then a predetermined amount of blood is collected by mounting a needle assembly at the tapered end of the first syringe 10. The volume ratio of anticoagulant to the drawn blood may be 1: 9 to effectively anticoagulate and maintain the stability of blood components. For example, 10 ml to 100 ml of blood is collected and 1 ml to 10 ml of anticoagulant is withdrawn accordingly. Subsequently, the needle assembly on first syringe 10 is removed and the sheath is fitted, and first syringe 10 is gently shaken to mix the anticoagulant and blood in first syringe 10 to obtain anticoagulated blood. The first syringe 10 is transferred to a centrifuge tube designed to match the shape and size of the first syringe 10 while keeping the conical head facing downward, and a balancing syringe containing water or blood and having the same specification as the first syringe 10 containing anticoagulated blood is further placed in another centrifuge tube of the centrifuge to achieve balancing and ensure the stability of the centrifugation process. Subsequently, the centrifuge is controlled to perform a centrifugation movement for 5 to 45 minutes at a relative centrifugal force ranging from 300 to 3000 g. The anticoagulated blood in the first syringe 10 is divided into a clear PPP layer, a PRP layer and a red blood cell layer from top to bottom. Among them, the separation is not clear enough when the relative centrifugal force or the centrifugal time is too short, and the blood cells are destroyed when the relative centrifugal force or the centrifugal time is too long, so that the conditions of hemolysis, platelet deformation or rupture and the like occur. Preferably, the centrifugation is carried out at a relative centrifugal force of 500g to 1500g for 15 minutes to 45 minutes, or at a relative centrifugal force of 1501g to 2500g for 10 minutes to 30 minutes, or at a relative centrifugal force of 2501g to 3500g for 5 minutes to 15 minutes.

The conical head of the first syringe 10 is held down on the split frame to ensure that the assembly process is stable to avoid undesired mixing of the layered components within the first syringe 10 and interference with the PRP located in the middle layer. The sheath of the first syringe 10 is detached and the three-way valve is sealingly connected to the conical head of the first syringe 10 via a luer fitting, and the collection container 12 and the second syringe 13 are sealingly connected at the other two ends of the three-way valve via luer fittings, respectively. The three-way valve is adjusted to communicate the first syringe 10 with the collection container 12, and the core rod of the first syringe 10 is pushed downward to transfer the lowermost red blood cells in the first syringe 10 into the collection container 12 through the communication member 11. Preferably, the first syringe 10 transfers the layer of contained red blood cells into the collection container 12 at a rate not exceeding 0.5 ml per second to smoothly discharge the red blood cells and reduce interference with the PRP layer, thereby stably ensuring recovery of platelets. Here, since there is a transition layer between the PRP layer and the red blood cell layer in the first syringe 10, it is preferable to transfer a part of the red blood cell layer in the first syringe 10 to the collection container 12 while leaving a part of the red blood cell layer in the first syringe 10, particularly 3% to 10% of the volume of the blood collected in the first syringe 10, in order to secure extraction concentration and recovery rate of PRP as much as possible.

Keeping the conical head of the first syringe 10 downward, adjusting the three-way valve to communicate the first syringe 10 with the second syringe 13, pushing the core rod of the first syringe 10 downward, and transferring the PRP layer in the first syringe 10 into the second syringe 13, so that only the PRP layer is contained in the second syringe 13 to extract the PRP. Preferably, the time taken for the first syringe 10 to transfer the PRP layer into the second syringe 13 exceeds 10 seconds, so as to avoid adhesion of the tunica albuginea layer after blood centrifugation to the red blood cells remaining on the vessel wall due to an excessively fast separation speed, thereby reducing interference on the PRP layer, stably performing extraction of PRP, and further ensuring stable platelets and recovery rate in PRP. Subsequently, the second syringe 13 is detached from the three-way valve and the sheath is fitted at the conical head.

Illustratively, the inventors have tested that in the case of 10 ml of blood collected by the first syringe 10 under the same centrifugation conditions, the first syringe 10 retains 0.3 ml to 1 ml of red blood cells transferred with the PRP layer into the second syringe 13, and the PRP transferred from the first syringe 10 into the second syringe 13 is 1 ml to 2 ml. In the case of 20 ml of blood collected through the first syringe 10, 0.6 ml to 2 ml of red blood cells retained by the first syringe 10 are transferred to the second syringe 13 along with the PRP layer, and the PRP transferred from the first syringe 10 to the second syringe 13 is 2 ml to 4 ml. In the case of collecting 30 ml of blood through the first syringe 10, the first syringe 10 retains 1 ml to 3 ml of red blood cells and the PRP layer is transferred into the second syringe 13, and the PRP transferred from the first syringe 10 into the second syringe 13 is 3 ml to 6 ml. In the case of collecting 50 ml of blood through the first syringe 10, 1.5 ml to 5 ml of red blood cells retained by the first syringe 10 are transferred to the second syringe 13 along with the PRP layer, and the PRP transferred from the first syringe 10 to the second syringe 13 is 5 ml to 10 ml. In the case of collecting 100 ml of blood through the first syringe 10, the first syringe 10 retains 3 ml to 10 ml of red blood cells and the PRP layer is transferred into the second syringe 13, and the PRP transferred from the first syringe 10 into the second syringe 13 is 10 ml to 20 ml.

In one specific example, the first syringe 10 collects 30 milliliters of blood and is centrifuged at a relative centrifugal force of 2200g for 20 minutes. 1 ml to 3 ml of red blood cells are retained in the first syringe 10 and transferred with the PRP layer into the second syringe 13. the PRP transferred from the first syringe 10 into the second syringe 13 may be 3 ml to 6 ml. In the case where the PRP extracted in the second syringe 13 was 5 ml, the average values of the platelet count in 30 ml of blood in the first syringe 10, the platelet count in 5 ml of PRP transferred to the second syringe 13, the platelet count in 19 ml of PPP left in the first syringe 10, and the platelet count in 5.8 ml of red blood cells transferred to the collection container 12 were (602 ± 54) × 10, respectively, as observed by an optical microscope⁹size/L, (2903 +/-91) × 10⁹Per liter, (130 +/-1) multiplied by 10⁹Per liter, (69 +/-1) multiplied by 10⁹And (2) per liter. The ratio of the platelet count of PRP to that of blood was calculated to obtain an enrichment factor, i.e., (2903X 10)⁹)/(602×10⁹) 4.80. In addition, the actual number of platelets in PRP, i.e., 5X 10, was calculated from the platelet count and PRP volume of PRP^-3×2903×10⁹And (4) respectively. The actual number of platelets in the blood, i.e. 30 x 10, was calculated from the platelet count and blood volume of the blood^-3×602×10⁹And (4) respectively. Finally, the recovery of platelets, i.e., (5X 2903X 10) is obtained from the actual number of platelets in the PRP and the actual number of platelets in the blood⁹)/(30×602×10⁹) 80.4%. In conclusion, the device according to the utility model controls the transfer of the first syringe 10 to the second injectionThe volume in the ejector 13 can adjust the final platelet enrichment coefficient, and the PRP obtained by extraction has higher platelet recovery rate and better extraction effect.

Furthermore, the time required to prepare PRP using a prior art apparatus for extracting PRP using a single centrifugation technique generally takes 15 minutes, and accordingly the platelet enrichment factor is approximately determined at 5 and the platelet recovery rate is approximately 60% or 62%. The time for preparing PRP using the apparatus for extracting PRP by the double centrifugation technique usually takes 20 minutes, and accordingly the platelet enrichment factor is roughly determined to be 6.40 ± 1.06 or 7.6 ± 1.2 and the platelet recovery rate is roughly (60.85 ± 8.97)% or (80.3 ± 12.8)%. In contrast, the device of the present invention for PRP preparation uses a single centrifugation technique, typically 15 minutes for preparation time, adjustable enrichment factor in the range of 3 to 10, and approximately (82 ± 14.5)%.

The thrombin is prepared by drawing a physiological saline solution using the third syringe 14, mounting a sheath on the tip of the third syringe 14, gently shaking the third syringe 14, and mixing the physiological saline solution and thrombin in the third syringe 14 to obtain the coagulant. The conical heads of the second syringe 13 containing the PRP and the third syringe 14 containing the coagulant are placed side by side with the conical heads facing down on the support frame 151, and a push plate 152 is attached to the head of the core rod of the second syringe 13 and the third syringe 14. The sheaths at the conical heads of the second injector 13 and the third injector 14 are detached, two liquid inlets of the spraying tee are respectively connected to the conical heads of the second injector 13 and the third injector 14 through luer connectors, and a nozzle with a built-in spraying head is connected to a liquid outlet of the spraying tee. The operator may then push the push plate 152 downward to push the second syringe 13 and the third syringe 14 core pins, and the PRP in the second syringe 13 and the coagulant in the third syringe 14 are pushed into the nozzle where they mix to produce a PRP gel, which is finally atomized by the spray head.

It should be understood that any combination of the operation steps of the extraction device in this embodiment to extract PRP should fall within the protection scope of the present invention.

It should be understood that although the description is in terms of various embodiments, not every embodiment includes only a single embodiment, and such description is for clarity purposes only, and those skilled in the art will recognize that the embodiments described herein may be combined as suitable to form other embodiments, as will be appreciated by those skilled in the art.

The above description is only an exemplary embodiment of the present invention, and is not intended to limit the scope of the present invention. Any equivalent alterations, modifications and combinations can be made by those skilled in the art without departing from the spirit and principles of the utility model.

Claims (7)

1. A device for extracting platelet rich plasma from blood, said device comprising:

a first syringe (10) which can contain anticoagulated blood and can centrifuge the anticoagulated blood for 5 to 45 minutes at a relative centrifugal force of 300 to 3000g in a state that a conical head is downward so that contents in the first syringe (10) are layered into a platelet poor plasma layer, a platelet rich plasma layer and a red blood cell layer from top to bottom;

a communication piece (11), wherein the first injector (10) is detachably connected to the communication piece (11) in a conical head-down posture;

a collection container (12) detachably connected to the communication member (11) so that the first syringe (10) and the collection container (12) are communicated via the communication member (11), and the erythrocyte layer in the first syringe (10) can be transferred into the collection container (12) via the communication member (11);

a second syringe (13) detachably connected to the communication member (11) so that the platelet rich plasma layer in the first syringe (10) can be transferred into the second syringe (13) through the communication member (11) to extract platelet rich plasma from the second syringe (13) only containing the platelet rich plasma layer;

a spraying device (15), wherein the second syringe (13) containing platelet-rich plasma is detachably connected to the spraying device (15) after being detached from the communicating piece (11);

and a third syringe (14) which can contain a coagulant and is detachably connected with the spraying device (15) so that the second syringe (13), the third syringe (14) and the spraying device (15) are in fluid communication, and the platelet-rich plasma in the second syringe (13) and the coagulant in the third syringe (14) can be transferred to the spraying device (15) and mixed in the spraying device (15) to generate platelet-rich gel and then be sprayed out.

2. Device according to claim 1, characterized in that the conical heads of the first syringe (10), the collection container (12), the second syringe (13) and the third syringe (14) are each detachably connected with a sheath.

3. Device according to claim 1, characterized in that the communication means (11) is a two-way valve or a three-way valve.

4. The device according to claim 1, characterized in that said spraying means (15) comprise:

a holding frame (151) in which the second syringe (13) and the third syringe (14) are held juxtaposed to each other;

a push plate (152) mounted to the heads of the second syringe (13) and the third syringe (14) core rod;

the spray tee joint comprises two liquid inlets and one liquid outlet, and the two liquid inlets of the spray tee joint are respectively connected to conical heads of the second injector (13) and the third injector (14);

a nozzle connected to the outlet of the spray tee to communicate the second syringe (13) and the third syringe (14) with the nozzle, wherein the platelet rich in the second syringe (13) and the coagulant in the third syringe (14) are transferred into the nozzle to mix to produce a gel in the nozzle;

and the spray head is arranged in the nozzle to spray the atomized gel.

5. Device according to claim 1, characterized in that the conical heads of the first syringe (10), of the second syringe (13) and of the third syringe (14) are removably connectable with a sheath.

6. Device according to claim 1, characterized in that the first syringe (10) is connected to the communication via a sealed connection.

7. The device of claim 6, wherein the sealing connection is configured as a luer fitting.

Images