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CN214077269U - PRP extraction element - Google Patents

PRP extraction element Download PDF

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CN214077269U
CN214077269U CN202022576563.9U CN202022576563U CN214077269U CN 214077269 U CN214077269 U CN 214077269U CN 202022576563 U CN202022576563 U CN 202022576563U CN 214077269 U CN214077269 U CN 214077269U
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blood
prp
speed limiting
container
limiting device
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李罗浩
李鉴墨
邓欣欣
郭丽娜
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Kangxi Biomedical Shenzhen Co ltd
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Abstract

The utility model relates to a PRP extraction device, which comprises a blood collection device, wherein the outlet of the blood collection device is detachably and hermetically connected with a speed limiting device which can be communicated with the blood collection device and controls the flow speed of blood; the speed limiting device is detachably and hermetically connected with the blood collecting device at the inlet end, and is detachably and hermetically connected with the blood collecting container at the outlet end. The utility model discloses a simple structure, low cost utilizes the speed limit principle to realize the accurate control to component transfer in the blood, and the use is simple and convenient, has solved the manual work and has drawed PRP's inconvenience, has realized the stable extraction to PRP.

Description

PRP extraction element
Technical Field
The utility model relates to a medical instrument, especially a PRP extraction element.
Background
The blood is divided into three layers after centrifugation, namely a Platelet-poor Plasma (PPP) layer, a Platelet-Rich Plasma (PRP) layer and a red blood cell layer from top to bottom, wherein the PRP has the functions of accelerating wound healing and repairing cartilage injury, can reduce the formation of surgical scars and promote cartilage regeneration, and is widely applied to the field of medical treatment. The traditional PRP manual extraction device is difficult to accurately control when components are injected in a bolus manner, and the problems that the quality of the prepared PRP is unstable, the deviation of the concentration of platelets in the PRP is large and the like can be caused. During PRP separation and extraction, researches show that platelets are concentrated in an intermediate layer with extremely small volume, and platelets with high concentration exist in the upper layer of red blood cells, so that the intermediate layer is extremely easy to disturb due to instability of operation in the transfer process, particularly when the component transfer process is fast, and the platelets are dispersed in a system, thereby causing failure in the extraction process or poor extraction effect. In order to improve the recovery rate of the platelets, a part of red blood cells are always reserved, but the red blood cells are not helpful for treatment; in some cases, complicated equipment is used to replace the traditional manual separation method, such as a bolus pump and a blood cell separation device to realize stable component transfer, but such a method excessively complicates the PRP extraction device, additionally increases higher cost, is very inconvenient to operate, and is not favorable for clinical application and popularization of the PRP extraction device.
SUMMERY OF THE UTILITY MODEL
To present not enough, the utility model provides a PRP extraction element that simple structure, convenient operation can stably accurately draw.
The utility model provides a technical scheme that its technical problem adopted is: a PRP extraction device comprises a blood collection device, wherein an outlet of the blood collection device is detachably and hermetically connected with a speed limiting device which can be communicated with the blood collection device and controls the flow speed of blood; the speed limiting device is detachably and hermetically connected with the blood collecting device at the inlet end, and is detachably and hermetically connected with the blood collecting container at the outlet end.
Preferably, the speed limiting device comprises a conduit and a micro-flow regulator fixedly arranged on the conduit and used for regulating the liquid flow, and two ends of the conduit are correspondingly detachably connected with the blood collecting device and the blood collecting container.
Preferably, the conduit is a flexible hose with elasticity, and the micro-flow regulator comprises a shell with a through hole and a regulating wheel for limiting speed; the shell is provided with a sliding chute which is communicated with the through hole and is provided with a guide inclined plane; the catheter penetrates through the through hole and is correspondingly connected with the blood collection device and the blood collection container at two ends of the catheter; the adjusting wheel is arranged in the sliding groove in a sliding mode along the guide inclined plane, the adjusting wheel is abutted to the guide pipe when the adjusting wheel is located at the highest point of the guide inclined plane, and the height difference of the guide inclined plane is not smaller than the diameter of the guide pipe.
Preferably, the speed limiting device is an injection needle with a tube diameter smaller than the diameter of the port at the outlet of the blood collection device.
Preferably, the diameter of the injection needle is in the range of 0.4-1.2 mm.
Preferably, the blood collection container comprises a red blood cell collection container, a PRP collection container, a PPP collection container.
Preferably, the PRP collection container comprises a container body with a cylindrical structure, a piston arranged in the container body in a matching and sliding manner, and a piston rod fixedly connected to the piston; the piston rod extends out of the container body; the container is characterized in that one end of the container body, which is far away from the piston rod, is detachably and hermetically connected with a switch piece, and the switch piece is detachably and hermetically connected with the speed limiting device or the blood collecting device and is used for switching on or off the communication between the speed limiting device or the blood collecting device and the container body.
Preferably, the switch member is a multi-way valve.
Preferably, the volume of the blood collection device is 10-100ml, and the volume of the PRP collection container is 5-50 ml.
Preferably, the blood collection device comprises a cylindrical device body and a sliding piece which is matched and slidably arranged in the device body and extends out of one end of the device body, the other end of the device body is constructed into a luer male joint or a luer female joint structure, and the inlet end of the speed limiting device corresponds to the luer female joint or the luer male joint matched with the speed limiting device.
The beneficial effects of the utility model reside in that: the utility model discloses a through in collecting blood collection device with blood, implement the centrifugation to blood collection device to come the stable transfer to each component in the blood after the centrifugation with the help of the speed limiter, thereby realize PRP's stable accurate and draw. The whole device has simple structure and low cost, can be realized by utilizing the speed limiting principle, has simple and convenient use process, realizes the accurate control of the injection process, also solves the inconvenience of manually extracting PRP, and is beneficial to popularization in clinical application.
Drawings
FIG. 1 is a schematic structural diagram of a speed limiting device according to an embodiment of the present invention, which employs a micro-flow regulator and a conduit;
FIG. 2 is a schematic structural view of the speed limiting device according to the embodiment of the present invention;
FIG. 3 is a schematic structural view showing the direct connection between the blood collection device and the PRP collection container when the injection needle is used as the speed limiting device according to the embodiment of the present invention;
part names and serial numbers in the figure: 1-blood collection device 10-body 11-slide 2-speed limiting device 20-conduit 21-micro flow regulator 22-injection needle 3-blood collection container 30-PRP collection container 300-container body 301-piston 302-piston rod.
Detailed Description
To clearly illustrate the objects, technical solutions and advantages of the embodiments of the present invention, the present invention will be further described in detail with reference to the accompanying drawings and embodiments, which are clearly and completely described below. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention. Furthermore, directional terms as referred to in the present disclosure, such as "upper", "lower", "front", "rear", "left", "right", "inner", "outer", etc., refer only to the direction of the attached drawings, and directional terms used are intended to better and more clearly illustrate and understand the present invention rather than to indicate or imply the orientation the present invention must have, and therefore should not be construed as limiting the present invention.
Embodiments of the present invention are shown in fig. 1 to 3, a PRP extraction device, comprising a blood collection device 1, the blood collecting device 1 can adopt a conventional syringe to collect blood conveniently and centrifugally stratify the blood after collection, blood does not need to be transferred in the process, pollution caused by blood transfer can be avoided, the outlet of the blood collecting device 1 is detachably and hermetically connected with a speed limiting device 2 which can be communicated with the blood collecting device 1 and controls the flow speed of blood, namely, the speed limiting device 2 and the blood collecting device 1 are detachable, when the blood sample is collected and layered, the blood sample and the speed limiting device are disconnected, after the blood sample is collected and layered, the speed limiting device 2 and the blood collecting device 1 are connected and conducted, the blood in the blood sampling device 1 can flow into the speed-limiting device 2, and the sealed connection avoids the leakage of the blood when flowing into the speed-limiting device 2 and the pollution of foreign substances to the blood; the speed limiting device 2 is detachably and hermetically connected with the blood collecting device 1 at the inlet end, and the blood collecting container 3 is detachably and hermetically connected at the outlet end, so that the blood collecting device 1 and the blood collecting container 3 are arranged at two ends of the speed limiting device 2, the blood flowing out of the speed limiting device 2 can be controlled by using the speed limiting device 2, and layered blood can be conveniently separated and extracted layer by layer. When in use, firstly, the blood collecting device 1 collects blood samples and centrifuges the blood samples, and then the blood is divided into three layers of PPP, PRP and red blood cells; then, under the condition of no speed limit, namely under the condition that the speed limiting device 2 is not started, pushing out a red blood cell layer with a certain volume, and when red blood cells with the total volume of 10-20% of the residual blood volume are left, starting the speed limiting device 2 to stably push out the red blood cells and keep the red blood cells with the total volume of 4-10% of the blood volume; finally, the PRP layer is stably transferred to a collection container under the control of the rate limiting device 2, and thus, extraction of PRP is completed. The whole extraction process avoids the blood from being exposed in the air, and effectively avoids the blood from being polluted. The extracted PRP has less residual quantity of red blood cells, can realize accurate control of component transfer quantity in the PRP extraction process, can accurately extract PRP within a certain platelet concentration range according to the use requirement of the PRP, realizes concentration customization of the PRP, improves the success rate and stability of extraction compared with the traditional manual PRP extraction, and reduces human errors; compared with the use of push injection pump equipment, the extraction process is greatly simplified, the device is simple, the operation is easy, the cost is low, and the popularization in clinical application is facilitated.
Further, as shown in fig. 1, the speed limiting device 2 may be implemented in a variety of different manners, the first manner is that the speed limiting device 2 includes a conduit 20 and a micro flow regulator 21 fixedly disposed on the conduit 20 for regulating the liquid flow, two ends of the conduit 20 are detachably connected to the blood collecting device 1 and the blood collecting container 3, the conduit 20 forms a blood flow channel, the micro flow regulator 21 is used for regulating the flow speed of blood in the conduit 20, the centrifugally layered blood can be separated and extracted layer by controlling the flow speed of blood, and the micro flow regulator 21 may be various regulating valves for regulating the liquid flow, such as an electromagnetic valve. For convenience, the conduit 20 is an elastic hose, and the relative position between the hose and the blood collection device 1 and the blood collection container 3 can be adjusted at will according to actual use conditions, so that the use in clinic is more facilitated, and the micro-flow regulator 21 comprises a shell with a through hole and a regulating wheel for limiting speed; the shell is provided with a sliding chute which is communicated with the through hole and is provided with a guide inclined plane; the catheter 20 penetrates through the through hole and is correspondingly connected with the blood collection device 1 and the blood collection container 3 at two ends of the catheter 20; the adjusting wheel is arranged in the sliding groove in a sliding mode along the guide inclined plane, the adjusting wheel abuts against the catheter 20 when being located at the highest point of the guide inclined plane, the height difference of the guide inclined plane is not smaller than the diameter of the catheter 20, the structure is simpler, and the adjusting wheel is more convenient to use, so that a structure similar to a flow regulator on an infusion apparatus is formed, the catheter 20 penetrates through a through hole of the shell, namely the shell is sleeved on the catheter, and the adjusting wheel can move up and down on the catheter according to the use condition, so that the adjusting wheel is located at a position which is most convenient to adjust and use; the adjusting wheel is arranged in the sliding groove and slides along the guide inclined plane, when the adjusting wheel is positioned at the highest point of the guide inclined plane, the adjusting wheel is pressed on the guide pipe, when the adjusting wheel slides down along the guide inclined plane, the adjusting wheel gradually extrudes the guide pipe 20, the guide pipe 20 generates elastic deformation, the flow passage of blood in the guide pipe 20 is gradually reduced, the flow velocity of the blood in the guide pipe 20 is further reduced, and therefore each layer of blood can be stably and accurately separated and extracted. The height difference of the guide inclined plane determines that the adjusting wheel generates displacement in the vertical direction in the sliding process so that the catheter can be in a non-extruded completely-communicated state or an extruded completely-closed state, and the control and adjustment of the blood flowing or static state are ensured.
The procedure used in the examples is as follows:
(1) blood collection device 1 trim: taking two 30mL blood collection devices 1 with the same specification, respectively sucking equal volumes of human blood and water (about 30mL), and fixing the blood collection devices in a centrifuge tube;
(2) first centrifugation: centrifuging at 2500rpm for 20min, and observing separation effect;
(3) separating red blood cells: connecting and fixing the centrifuged blood collection device 1 and one end of a speed limiting device 2, connecting and fixing the other end of the speed limiting device 2 and a blood collection container 3, discharging most of red blood cells under the condition of unlimited speed, then adjusting the speed limiting device 2 to control the flow speed of blood, and continuously propelling and keeping 4-8% of the red blood cells;
(4) and (3) second centrifugation: taking down the blood collection device 1, centrifuging for 10min at the rotating speed r of 3300rpm, and observing the separation effect;
(5) PRP separation: the blood collection device 1 after the second centrifugation is fixedly connected with the speed limiting device 2 again, meanwhile, the other end of the speed limiting device 2 is replaced with the blood collection container 3 for collecting PRP and is fixedly connected with the speed limiting device, then the flow speed of blood is regulated and controlled through the speed limiting device 2, the PRP layer is pushed into the blood collection container 3 for collecting PRP, the separation effect is observed, at the moment, when the blood collection container 3 is of a structure similar to an injector, the blood collection device 1 can be directly connected with the blood collection container 3, the speed limiting device 2 does not need to be connected, and the use is more convenient.
(6) PRP extraction: the blood collection vessel 3 in which the PRP was collected was removed and shaken well to obtain the PRP which was isolated and extracted.
As shown in fig. 2, in the second mode, the speed limiting device 2 is an injection needle 22 with a diameter smaller than the diameter of the port at the outlet of the blood collection device, so that the flow channel of blood becomes smaller, the flow speed of blood flowing from the large-diameter channel to the small-diameter channel becomes slower, the speed becomes slower, the disturbance between layers is avoided, meanwhile, the red blood cells at the lower layer can be maximally discharged through the small-diameter channel, the excessive red blood cells are prevented from remaining in the PRP, the speed limiting effect on the flow of blood can be also realized through the injection needles 22 with different diameters, and the residual quantity of the red blood cells in the PRP is reduced. In practical use, the injection needle 22 is used to allow blood to flow into the small-bore passage after flowing out of the blood collection device 1, so that no PRP enters the injection needle 22 when red blood cells are discharged, and the injection needle 22 can be removed after red blood cells are discharged to directly connect the blood collection device 1 and the blood collection container 3 for PRP extraction, which is more convenient to use. In this case, for practical convenience, the diameter of the injection needle 22 is in the range of 0.4-1.2mm, and the injection needle 22 in this range is also a commonly used injection needle, so that a complicated instrument is not required, and clinical use is facilitated.
In the embodiment, the extraction of PRP is realized by selecting the injection needle 22 with the model of 0.8mm × 30mm as the speed limiting device 2, and selecting the injection needle 22 with the model of 1.2mm × 30mm as the comparison, the process is as follows:
(1) blood sampling syringe 1 trimming: taking two 30mL blood collection devices 1 with the same specification, respectively sucking equal volumes of human blood and water (about 30mL), and fixing the blood collection devices in a centrifuge tube;
(2) centrifuging: centrifuging for 40min at the rotating speed r of 2500rpm, and observing the separation effect;
(3) separating red blood cells: connecting and fixing the centrifuged blood sampling injector 1 with an injection needle 22, connecting and fixing the injection needle 22 with the blood collection container 3, and continuously propelling and retaining 4-8% of red blood cells after most of red blood cells are discharged;
(4) PRP separation: the needle 22 was removed, another blood collection container 3 for collecting PRP was attached and fixed to the blood collection device 1, and the PRP layer was pushed into the blood collection container 3 for collecting PRP to observe the separation effect.
(6) PRP extraction: the blood collection vessel 3 in which the PRP was collected was removed and shaken well to obtain the PRP which was isolated and extracted.
The experimental procedures and results for a 0.8mm by 30mm needle, a 1.2mm by 30mm needle were as follows:
table 1 shows the comparison between the actual operation of a 0.8mm by 30mm injection needle and that of a 1.2mm by 30mm injection needle; table 2 shows platelet data from PRP extraction using a 0.8mm x 30mm needle; table 3 shows platelet data from PRP extraction using a 1.2mm x 30mm needle;
Figure BDA0002769108040000081
TABLE 1
Figure BDA0002769108040000091
TABLE 2
Figure BDA0002769108040000092
TABLE 3
Wherein, when the needle tube type (0.8mm multiplied by 30mm) is selected for extracting PRP:
the enrichment factor is (3526.5 × 109)/(854.5 × 109) ═ 4.1
The recovery rate was (1.5 × 3526.5 × 109)/(10 × 854.5 × 109): 61.9%
When the needle tube type (1.2mm multiplied by 30mm) is selected for extracting PRP:
the enrichment factor is (3154.5 × 109)/(854.5 × 109) ═ 3.7
The recovery rate was (1.5 × 3154.5 × 109)/(10 × 854.5 × 109): 55.4%
The concentration of PRP in clinical use generally requires that the enrichment coefficient is more than 3, and the recovery rate is the most main index for evaluating the PRP extraction device, for example, the recovery rate of platelet of the Weigao Platelet Rich Plasma (PRP) preparation set which is the most widely used clinical application at present is 60%. From the data, the enrichment factor and the recovery rate of the extracted PRP are better by using a preparation tool with a thinner needle tube diameter.
In a further modification, as shown in fig. 1 to 3, in order to distinguish and facilitate the collection of the centrifuged layers of blood, the blood collection container 3 includes a red blood cell collection container, a PRP collection container 30, a PPP collection container, which can adopt a conventionally used blood bag as a collection container; in order to facilitate the use of the PRP, the PRP collection container 30 comprises a container body 300 with a cylindrical structure, a piston 301 matched and slidably arranged in the container body 300, and a piston rod 302 fixedly connected to the piston 301, wherein the piston rod 302 extends out of the container body 300, so that the PRP collection container 30 becomes a syringe, the PRP collection container is convenient to use after being collected, the steps of transferring between different containers are reduced, and the risk of contamination of the PRP collection container is reduced; the end of the container body 300 far away from the piston rod 302 is detachably and hermetically connected with a switch 4, the on-off of the PRP collection container 30 can be controlled by the switch 4, and at this time, the switch 4 is a multi-way valve, such as a conventionally used two-way valve or three-way valve structure, so that the manufacturing cost is greatly reduced, and in addition, in order to visually observe and control the extraction process, the multi-way valve is made of a transparent material, such as plastic or glass; the switch piece 4 is detachably and hermetically connected with the speed limiting device 2 or the blood collecting device 1 and is used for switching on or off the communication between the speed limiting device 2 or the blood collecting device 1 and the container body 300, so that the switch piece 4 is connected with the speed limiting device 2 according to different used speed limiting devices 2 to switch on or off a blood flowing channel, for example, the application when the micro-flow regulator 21 and the conduit 20 are used as the speed limiting device 2; alternatively, the switch member 4 may be directly connected to the blood sampling device 1, in which case the PRP does not flow into the speed limiter 2 after the red blood cells are discharged by the speed limiter 2, for example, the use of the injection needle 22 as the speed limiter 2 may be employed, but the injection needle 22 may be connected to the PRP collection container 30 depending on the use condition and the PRP collection container 30 to be used. The volume of the blood collection device 1 is 10-100ml, and the volume of the PRP collection container 30 is 5-50ml, so that the daily use requirement is met without waste, for example, when the specified blood volume is 10ml, the volume of the collected PRP is 1-3ml, and the volume of the collected PRP is 5 ml.
In a further improvement, as shown in fig. 1 to 3, the blood collection device 1 includes a cylindrical body 10, a sliding member 11 that is slidably fitted in the body 10 and extends from one end of the body 10, the other end of the body 10 is of a luer male joint or luer female joint structure, the inlet end of the speed limiting device 2 corresponds to the luer female joint or luer male joint that is matched with the speed limiting device, that is, the sliding member 11 slides in the body 10, and the end of the sliding member extends from the body 10, the body 10 of the blood collection device 1 is a syringe of a common syringe, the sliding member 11 is a piston rod and a piston, the two ends of the body 10 correspond to the sliding member 11 and the speed limiting device 2, the body 10 and the speed limiting device 2 are connected through the luer joints, and pushing the sliding member 11 to move towards the speed limiting device 2 pushes blood sucked in the body 10 out, ruhr joint connection is simple and convenient, is favorable to the dismouting between speed limiting device 2 and blood collection system 1.
It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are considered to be within the scope of the invention as defined by the following claims.

Claims (10)

1. A PRP extraction device, characterized by: the device comprises a blood collecting device, wherein the outlet of the blood collecting device is detachably and hermetically connected with a speed limiting device which can be communicated with the blood collecting device and controls the flow speed of blood; the speed limiting device is detachably and hermetically connected with the blood collecting device at the inlet end, and is detachably and hermetically connected with the blood collecting container at the outlet end.
2. The PRP extraction device according to claim 1, wherein: the speed limiting device comprises a guide pipe and a micro-flow regulator fixedly arranged on the guide pipe and used for regulating the liquid flow, and two ends of the guide pipe are correspondingly detachably connected with the blood collecting device and the blood collecting container.
3. The PRP extraction device according to claim 2, wherein: the conduit is an elastic hose, and the micro-flow regulator comprises a shell provided with a through hole and a regulating wheel for limiting speed; the shell is provided with a sliding chute which is communicated with the through hole and is provided with a guide inclined plane; the catheter penetrates through the through hole and is correspondingly connected with the blood collection device and the blood collection container at two ends of the catheter; the adjusting wheel is arranged in the sliding groove in a sliding mode along the guide inclined plane, the adjusting wheel is abutted to the guide pipe when the adjusting wheel is located at the highest point of the guide inclined plane, and the height difference of the guide inclined plane is not smaller than the diameter of the guide pipe.
4. The PRP extraction device according to claim 1, wherein: the speed limiting device is an injection needle with the pipe diameter smaller than the diameter of the port at the outlet of the blood collecting device.
5. The PRP extraction device according to claim 4, wherein the diameter of the injection needle is in the range of 0.4-1.2 mm.
6. The PRP extraction device according to claim 1, wherein the blood collection container comprises a red blood cell collection container, a PRP collection container, and a PPP collection container.
7. The PRP extraction device according to claim 6, wherein the PRP collection container comprises a container body with a cylindrical structure, a piston matched and slidably arranged in the container body, and a piston rod fixedly connected to the piston; the piston rod extends out of the container body; the container is characterized in that one end of the container body, which is far away from the piston rod, is detachably and hermetically connected with a switch piece, and the switch piece is detachably and hermetically connected with the speed limiting device or the blood collecting device and is used for switching on or off the communication between the speed limiting device or the blood collecting device and the container body.
8. The PRP extraction device of claim 7, wherein the switch member is a multi-way valve.
9. The PRP extraction device according to claim 6, wherein the volume of the blood collection device is 10-100ml, and the volume of the PRP collection container is 5-50 ml.
10. The PRP extraction device according to claim 1, wherein the blood collection device comprises a cylindrical body, and a sliding member that is slidably fitted in the body and extends from one end of the body, the other end of the body is configured as a luer male connector or a luer female connector structure, and the inlet end of the speed limiting device corresponds to the luer female connector or the luer male connector that is matched with the speed limiting device.
CN202022576563.9U 2020-11-10 2020-11-10 PRP extraction element Active CN214077269U (en)

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