CN113828426A - Method and device for extracting platelet-rich plasma from blood - Google Patents
Method and device for extracting platelet-rich plasma from blood Download PDFInfo
- Publication number
- CN113828426A CN113828426A CN202111043303.8A CN202111043303A CN113828426A CN 113828426 A CN113828426 A CN 113828426A CN 202111043303 A CN202111043303 A CN 202111043303A CN 113828426 A CN113828426 A CN 113828426A
- Authority
- CN
- China
- Prior art keywords
- syringe
- platelet
- rich plasma
- layer
- blood
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000004623 platelet-rich plasma Anatomy 0.000 title claims abstract description 114
- 210000004369 blood Anatomy 0.000 title claims abstract description 58
- 239000008280 blood Substances 0.000 title claims abstract description 58
- 238000000034 method Methods 0.000 title claims abstract description 40
- 238000005507 spraying Methods 0.000 claims abstract description 43
- 238000004891 communication Methods 0.000 claims abstract description 42
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 35
- 239000000701 coagulant Substances 0.000 claims abstract description 20
- 210000002381 plasma Anatomy 0.000 claims abstract description 6
- 239000007921 spray Substances 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 15
- 238000012546 transfer Methods 0.000 claims description 15
- 239000003146 anticoagulant agent Substances 0.000 claims description 10
- 229940127219 anticoagulant drug Drugs 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 7
- 108090000190 Thrombin Proteins 0.000 claims description 6
- 229960004072 thrombin Drugs 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 239000010410 layer Substances 0.000 description 52
- 238000005119 centrifugation Methods 0.000 description 23
- 238000000605 extraction Methods 0.000 description 21
- 238000011084 recovery Methods 0.000 description 12
- 239000000306 component Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000007789 sealing Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 239000002504 physiological saline solution Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000000717 retained effect Effects 0.000 description 3
- 230000007704 transition Effects 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 206010007710 Cartilage injury Diseases 0.000 description 1
- 206010053567 Coagulopathies Diseases 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000012503 blood component Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000035602 clotting Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 239000011229 interlayer Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
- 230000037314 wound repair Effects 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B11/00—Feeding, charging, or discharging bowls
- B04B11/04—Periodical feeding or discharging; Control arrangements therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B11/00—Feeding, charging, or discharging bowls
- B04B11/06—Arrangement of distributors or collectors in centrifuges
Landscapes
- External Artificial Organs (AREA)
Abstract
The present invention relates to a method and a device for extracting platelet-rich plasma from blood. The method comprises the following steps: the conical head of a first syringe containing anticoagulated blood is downward, the first syringe is centrifuged for 5 to 45 minutes at a relative centrifugal force of 300 to 3000g, and the content in the first syringe is layered into a platelet poor plasma layer, a platelet rich plasma layer and a red blood cell layer from top to bottom; keeping the conical head of the first syringe facing downwards, and transferring the erythrocyte layer in the first syringe into a collection container through a communication part; keeping the conical head of the first syringe facing downwards, and transferring the platelet rich plasma layer in the first syringe into the second syringe through the communication piece, so that the second syringe only contains the platelet rich plasma layer; the platelet-rich plasma in the second syringe and the coagulant in the third syringe are transferred to the spraying device, and the platelet-rich plasma and the coagulant in the spraying device are mixed to generate platelet-rich gel which is sprayed out of the spraying device.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a method and a device for extracting platelet-rich plasma from blood.
Background
After centrifugation, the blood is divided into three layers, namely a Platelet-Poor Plasma (PPP) layer, a Platelet-Rich Plasma (PRP) layer and a red blood cell layer from top to bottom. Liquid PRP is generally injected to a treatment site to accelerate wound healing and repair cartilage damage, etc., and gel-like PRP is suitable for adhering, filling and covering wounds and preventing loss of growth factors, and thus is widely used in the medical field. The PRP is usually extracted by primary centrifugation or secondary centrifugation, but the primary centrifugation is usually performed by extracting the intermediate PRP layer, which causes the PRP in the intermediate layer to diffuse into the upper PPP layer or the lower erythrocyte layer, resulting in low PRP extraction concentration and recovery rate. The second centrifugation method requires two centrifugation processes, which obviously makes the required extraction process more complicated and time-consuming. In addition, the liquid PRP obtained by extraction is generally directly injected in the prior art, so the using effect is more general.
Therefore, there is a need in the art for an extraction method and apparatus that provides high extraction concentration and recovery, simple and time-consuming extraction process, and good utility.
Disclosure of Invention
The present invention aims to provide a method for extracting platelet-rich plasma from blood which can solve at least some of the above problems.
The present invention also aims to provide a device for extracting platelet-rich plasma from blood, applying the above improved method.
According to one aspect of the present invention, there is provided a method of extracting platelet rich plasma from blood, the method comprising: the method comprises the following steps of (1) enabling a conical head of a first syringe containing anticoagulated blood to face downwards, and centrifuging the first syringe for 5 to 45 minutes at a relative centrifugal force of 300 to 3000g, wherein the content in the first syringe is divided into a platelet poor plasma layer, a platelet rich plasma layer and a red blood cell layer from top to bottom; maintaining the first syringe with its conical tip facing downward and connecting the first syringe in fluid communication to a collection container via a communication member, such that the layer of red blood cells in the first syringe is transferred into the collection container via the communication member; maintaining the first syringe with its conical tip facing downward and connecting the first syringe in fluid communication to a second syringe via the communication member, and transferring the platelet rich plasma layer in the first syringe into the second syringe via the communication member such that the second syringe contains only the platelet rich plasma layer; connecting the second syringe containing platelet rich plasma and a third syringe containing a clotting agent in fluid communication to a spray device; and transferring the platelet-rich plasma in the second syringe and the coagulant in the third syringe to the spraying device, mixing the platelet-rich plasma and the coagulant in the spraying device to generate platelet-rich gel, and spraying the platelet-rich gel from the spraying device.
Compared with the prior art, the invention can ensure the clear layering of the blood in the first injector after centrifugal movement and avoid damaging blood cells by setting the relative centrifugal force and the centrifugal time range of the first injector for centrifugal movement. In addition, through assembling first syringe, UNICOM's piece, collection container and second syringe, only need carry out once centrifugal operation and can realize PRP's extraction, it is short and simple structure consuming time. The PRP can be transferred into the second injector by operating the first injector, which enables the bulk transfer of the PRP layer while reducing interlayer disturbances, thereby improving PRP recovery. In addition, by operating the advancing degree of the first plunger rod, a desired PRP concentration can be obtained as required, and the applicability is high. Finally, by connecting the second syringe and the third syringe through the spraying device, the PRP gel can be prepared, and the using effect of the prepared PRP is further improved.
Preferably, the anticoagulated blood is obtained by: drawing anticoagulant using the first syringe; collecting blood using the first syringe, the first syringe drawing a ratio of anticoagulant to collected blood of 1: 9; and mixing the anticoagulant and the blood in the first syringe to obtain anticoagulated blood.
Preferably, the first syringe collects blood in a volume ranging from 10 ml to 100 ml.
Preferably, the first syringe is centrifuged at a relative centrifugal force of 500g to 1500g for 15 minutes to 45 minutes.
Preferably, the first syringe is centrifuged at a relative centrifugal force of 1501g to 2500g for 10 minutes to 30 minutes.
Preferably, the first syringe is centrifuged at a relative centrifugal force of 2501g to 3500g for 5 minutes to 15 minutes.
Preferably, the first syringe transfers the layer of contained red blood cells into the collection container at a rate of no more than 0.5 ml per second.
Preferably, the first syringe transfers only a portion of the layer of red blood cells to the collection container, leaving a remaining portion of the layer of red blood cells within the first syringe.
Preferably, the volume of red blood cells left in the first syringe ranges from 3% to 10% of the volume of the collected blood.
Preferably, the time required for the first syringe to transfer the platelet rich plasma layer into the second syringe exceeds 10 seconds.
Preferably, the coagulant is obtained by: withdrawing saline using the third syringe; and (3) preparing thrombin by using the third syringe, and mixing the physiological saline in the third syringe with the thrombin to obtain the coagulant.
According to another aspect of the present invention, there is provided a device for extracting platelet rich plasma from blood for performing the aforementioned method, the device comprising: a first syringe containing anticoagulated blood and centrifuging the anticoagulated blood for 5 to 45 minutes at a relative centrifugal force of 300 to 3000g in a state that a conical head is downward, wherein the content in the first syringe is divided into a platelet poor plasma layer, a platelet rich plasma layer and a red blood cell layer from top to bottom; the first injector is detachably connected to the communicating piece in a conical head-down posture; a collection container detachably connected to the communication member so that the first syringe and the collection container are communicated via the communication member, and the erythrocyte layer in the first syringe is transferred into the collection container via the communication member; and the second syringe is detachably connected to the communicating piece, so that the platelet rich plasma layer in the first syringe is transferred into the second syringe through the communicating piece, and the platelet rich plasma layer is only contained in the second syringe to extract the platelet rich plasma. The second syringe containing platelet-rich plasma is detachably connected to the spraying device after being detached from the communicating piece; and a third syringe which contains a coagulant and is detachably connected with the spraying device so as to enable the second syringe, the third syringe and the spraying device to be communicated in a fluid mode, wherein the platelet-rich plasma in the second syringe and the coagulant in the third syringe can be transferred to the spraying device and mixed in the spraying device to generate platelet-rich gel and then the platelet-rich gel is sprayed out.
Preferably, the conical heads of the first syringe, the collection container, the second syringe and the third syringe are all detachably connected with sheaths.
Preferably, the communication member is a two-way valve or a three-way valve.
Preferably, the spray coating device comprises: a holding frame holding the second syringe and the third syringe juxtaposed to each other therein; a push plate mounted to the heads of the second syringe and the third syringe core rod; the spray tee joint comprises two liquid inlets and a liquid outlet, and the two liquid inlets of the spray tee joint are respectively connected to the conical heads of the second injector and the third injector; a nozzle connected to the liquid outlet of the spray tee to communicate the second and third syringes and the nozzle, wherein the platelet rich in the second syringe and the coagulant in the third syringe are transferred into the nozzle to mix to produce a gel in the nozzle; and the spray head is arranged in the nozzle to spray the atomized gel.
Additional features and advantages of the invention will be set forth in part in the description which follows, and in part will be apparent to those having ordinary skill in the art upon examination of the following, or may be learned from the practice of the invention.
Drawings
Embodiments of the invention are described in detail below with reference to the attached drawing figures, wherein:
FIG. 1 is a first schematic view of an apparatus for extracting platelet rich plasma from blood according to the present invention;
FIG. 2 is a second schematic view of an apparatus for extracting platelet rich plasma from blood according to the present invention;
fig. 3 is a schematic flow diagram of a method of extracting platelet rich plasma from blood according to the present invention.
Description of reference numerals:
10-a first syringe; 11-a communication member; 12-a collection vessel; 13-a second syringe; 14-a third syringe; 15-a spraying device; 151-a support frame; 152-push plate.
Detailed Description
Referring now to the drawings, illustrative embodiments of the disclosed method and apparatus for extracting platelet rich plasma from blood will be described in detail. Although the drawings are provided to present some embodiments of the invention, the drawings are not necessarily to scale of particular embodiments, and certain features may be exaggerated, removed, or partially sectioned to better illustrate and explain the present disclosure. The position of some components in the drawings can be adjusted according to actual requirements on the premise of not influencing the technical effect. The appearances of the phrase "in the drawings" or similar language in the specification are not necessarily referring to all drawings or examples.
Certain directional terms used hereinafter to describe the drawings, such as "inner", "outer", "above", "below", and other directional terms, will be understood to have their normal meaning and refer to those directions as they normally relate to when viewing the drawings. Unless otherwise indicated, the directional terms described herein are generally in accordance with conventional directions as understood by those skilled in the art.
The terms "first," "second," and the like, as used herein, do not denote any order, quantity, or importance, but rather are used to distinguish one element from another.
As used herein, the terms "joined," "connected," and the like, are intended to encompass both components which are indirectly joined together through intervening layers (e.g., adhesives, welds, etc.) or intermediate members (e.g., connectors, transitions, etc.), and components which are directly joined together without any intervening layers (e.g., adhesives, welds, etc.) or intermediate members (e.g., connectors, transitions, etc.).
Fig. 1 and 2 show a platelet-rich plasma extraction device of the present invention, which is simpler than the prior art extraction device, and can ensure the concentration of extracted PRP and improve the PRP usage effect while simplifying the extraction complexity and reducing the extraction time. The device may include, among other things, a first syringe 10, a communication 11, a collection container 12, a second syringe 13, a spray device 15, and a third syringe 14.
Specifically, the first syringe 10 includes a barrel, a core rod located on the inner wall of the barrel and capable of sliding along the inner wall of the barrel in a sealing manner, and a conical head located at one end of the barrel. Alternatively, the first syringe 10, the second syringe 13 and the third syringe 14 may also be medical devices having similar functions in the art. The communication member 11 may be a two-way valve or a three-way valve.
In case the communication member 11 is designed as a two-way valve, the two-way valve may comprise a two-way pipe, a two-way valve spool located in the two-way pipe and a rotary piston which may control the rotation of the two-way valve spool. The rotating piston is rotated to enable the channel in the two-way valve core to align with the two ends of the two-way pipe or enable the two-way valve core to block the two-way pipe, so that containers connected to the two ends of the two-way pipe are communicated or disconnected. Wherein the first syringe 10 and the collection container 12 may be detachably connected to both ends of a two-way valve, respectively, such that the first syringe 10 and the collection container 12 are connected or disconnected via the two-way valve, so that the contents in the first syringe 10 may be transferred into the collection container 12 or prevented from being transferred therebetween via the two-way valve by pushing the first syringe 10 core rod. In practice, the collection container 12 may be a syringe or a waste container. In the case where the collection container 12 is detached from the two-way valve, the second syringe 13 may be connected to the two-way valve instead of the collection container 12 to be connected to the first syringe 10 via the two-way valve, so that the contents in the first syringe 10 may be transferred into the second syringe 13 via the two-way valve by pushing on the first syringe 10 plunger. Alternatively, in the case where both the first syringe 10 and the collection container 12 are detached from the two-way valve, the first syringe 10 and the second syringe 13 may be respectively connected to both ends of a new unused two-way valve to communicate the first syringe 10 and the second syringe 13 via the new unused two-way valve, so that the transferred contents are not mixed with the remaining substance in the two-way valve during the transfer of the contents of the first syringe 10 into the second syringe 13.
In case the communication member 11 is designed as a three-way valve, the three-way valve may be configured to include a three-way pipe, a three-way spool located within the three-way pipe, and a rotary piston that may control the rotation of the three-way spool. The channel in the three-way valve core can be communicated with any two ports of the three-way pipe by rotating the rotary piston, so that any two containers connected to the three ports of the three-way pipe are communicated and disconnected with the other container. Wherein, the first syringe 10, the collection container 12 and the second syringe 13 can be respectively connected to three ends of a three-way valve, so that the rotary piston of the three-way valve is adjusted as required to enable the first syringe 10 to be communicated with the collection container 12 but not communicated with the second syringe 13, or the first syringe 10 is adjusted to be not communicated with the collection container 12 but communicated with the second syringe 13, and then the content in the first syringe 10 can be transferred into the collection container 12 or the second syringe 13 as required.
Alternatively, one end of the three-way valve may be designed as a vent end, and the first syringe 10 and the collection container 12 may be connected to the other two ends of the three-way valve, and the contents of the first syringe 10 may be transferred into the collection container 12 via the three-way valve by turning the rotary piston to close the vent end. The collection container 12 is then either three-way disconnected and the second syringe 13 is connected to the three-way valve instead of the collection container 12, or both the first syringe 10 and the collection container 12 are disconnected from the three-way valve and the first syringe 10 and the second syringe 13 are connected by a new unused three-way valve, so that the first syringe 10 and the third syringe 14 are connected via the three-way valve. The contents of the first syringe 10 are transferred into the second syringe 13 through the three-way valve by rotating the piston to close the vent end. Finally, the vented end is communicated to the second syringe 13 by rotating the plunger, pulling the second syringe 13 plunger stem to draw the remaining contents of the three-way valve stem into the second syringe 13. Optionally, the vent end of the three-way valve may be provided with a sterilizing filter membrane.
The second syringe 13 may be detachably connected to the spraying device 15 after being detached from the communicating member 11. The third injector 14 may be detachably connected to the spraying device 15, so that both the second injector 13 and the third injector 14 can be connected to the spraying device 15, and the contents of the second injector 13 and the third injector 14 are transferred into the spraying device 15 by pushing the core rods of the second injector 13 and the third injector 14, and are mixed in the spraying device 15 and then are sprayed out through the spraying device 15.
Alternatively, the spray coating device 15 may include a support frame 151, a thrust plate 152, a spray tee, a nozzle, and a spray head. The second syringe 13 and the third syringe 14 may be held in parallel on the holding frame 151. The spray tee may comprise two liquid inlets and one liquid outlet, wherein the two liquid inlets are respectively connected to the conical heads of the second injector 13 and the third injector 14, and the liquid outlet is connected to the nozzle. Thus, the contents of the second and third injectors 13, 14 can be transferred through the spray tee into the nozzle and mixed within the nozzle by pushing the push plate 152. The nozzle is also internally provided with a spray head so as to atomize and spray the mixture in the nozzle.
Optionally, the conical head of the first syringe 10 may be detachably connected with a sheath when detached from the communicating member 11, so as to prevent the contents of the first syringe 10 from being contaminated by the external environment. Similarly, the second syringe 13 may be detachably connected with a sheath when the communication member 11 and the spraying device 15 are detached, and the third syringe 14 may be detachably connected with a sheath when the spraying device 15 is detached.
Alternatively, the first syringe 10 may be connected to the communicating member 11 by a detachable sealing connection such as a luer fitting. Similarly, the second syringe 13 may be connected to the communication member 11 and the spraying device 15 by a detachable sealing connection, and the third syringe 14 may be connected to the spraying device 15 by a detachable sealing connection. In addition, the conical head of the first syringe 10 may also have a needle assembly removably attached thereto.
Alternatively, the first syringe 10, the communication member 11, the collection container 12, the second syringe 13 and the spraying device 15 may all be made of a transparent material such as plastic or glass, and the transfer of the substance from the first syringe 10 to the collection container 12 through the communication member 11, the transfer of the substance from the first syringe 10 to the second syringe 13 through the communication member 11, and the transfer of the substance from the second syringe 13 and the third syringe 14 to the spraying device 15 can all be clearly observed by the operator, so that the use of the extraction device of the present invention is convenient.
The process of PRP extraction and gel preparation using the extraction device of the present invention is schematically illustrated below with reference to fig. 3. It is noted that only the communication member 11 will be described below as a three-way valve as an example of the extraction method, and that the communication member 11 or other examples of the above-mentioned components may also be incorporated in the method below.
From the present viewpoint, it is generally considered that the recovery rate of PRP extracted by the secondary centrifugation technique is higher and PRP with a higher platelet concentration can be prepared than by the primary centrifugation technique. However, the inventors believe that the essence of extracting PRP is: sufficient centrifugation to ensure that cells are packed in a density gradient and tend to be constant, and disturbance reduction in separation of the PRP layer, therefore, the factor influencing the extraction of PRP is not to use a so-called primary centrifugation technique or a secondary centrifugation technique, but to influence the stable packing process of the centrifuged cells and whether disturbance is caused in collection of the corresponding components. Thus, the extraction efficiency of PRP by the secondary centrifugation technique is certainly better than that by the primary centrifugation technique. During the centrifugation process, the cell accumulation tends to be stable by adjusting the relative centrifugal force and the centrifugation time. In the PRP separation process, the reduced perturbation of the intermediate PRP layer prevents the platelets from spreading to the adjacent upper and lower layers, thereby collecting PRP with higher platelet recovery.
First, a predetermined amount of anticoagulant is drawn using the first syringe 10, and then a predetermined amount of blood is collected by mounting a needle assembly at the tapered end of the first syringe 10. The volume ratio of anticoagulant to the drawn blood may be 1:9 to effectively anticoagulate and maintain the stability of blood components. For example, 10 ml to 100 ml of blood is collected and 1 ml to 10 ml of anticoagulant is withdrawn accordingly. Subsequently, the needle assembly on first syringe 10 is removed and the sheath is fitted, and first syringe 10 is gently shaken to mix the anticoagulant and blood in first syringe 10 to obtain anticoagulated blood. The first syringe 10 is transferred to a centrifuge tube designed to match the shape and size of the first syringe 10 while keeping the conical head facing downward, and a balancing syringe containing water or blood and having the same specification as the first syringe 10 containing anticoagulated blood is further placed in another centrifuge tube of the centrifuge to achieve balancing and ensure the stability of the centrifugation process. Subsequently, the centrifuge is controlled to perform a centrifugation movement for 5 to 45 minutes at a relative centrifugal force ranging from 300 to 3000 g. The anticoagulated blood in the first syringe 10 is divided into a clear PPP layer, a PRP layer and a red blood cell layer from top to bottom. Among them, the separation is not clear enough when the relative centrifugal force or the centrifugal time is too short, and the blood cells are destroyed when the relative centrifugal force or the centrifugal time is too long, so that the conditions of hemolysis, platelet deformation or rupture and the like occur. Preferably, the centrifugation is carried out at a relative centrifugal force of 500g to 1500g for 15 minutes to 45 minutes, or at a relative centrifugal force of 1501g to 2500g for 10 minutes to 30 minutes, or at a relative centrifugal force of 2501g to 3500g for 5 minutes to 15 minutes.
The conical head of the first syringe 10 is held down on the split frame to ensure that the assembly process is stable to avoid undesired mixing of the layered components within the first syringe 10 and interference with the PRP located in the middle layer. The sheath of the first syringe 10 is detached and the three-way valve is sealingly connected to the conical head of the first syringe 10 via a luer fitting, and the collection container 12 and the second syringe 13 are sealingly connected at the other two ends of the three-way valve via luer fittings, respectively. The three-way valve is adjusted to communicate the first syringe 10 with the collection container 12, and the core rod of the first syringe 10 is pushed downward to transfer the lowermost red blood cells in the first syringe 10 into the collection container 12 through the communication member 11. Preferably, the first syringe 10 transfers the layer of contained red blood cells into the collection container 12 at a rate not exceeding 0.5 ml per second to smoothly discharge the red blood cells and reduce interference with the PRP layer, thereby stably ensuring recovery of platelets. Here, since there is a transition layer between the PRP layer and the red blood cell layer in the first syringe 10, it is preferable to transfer a part of the red blood cell layer in the first syringe 10 to the collection container 12 while leaving a part of the red blood cell layer in the first syringe 10, particularly 3% to 10% of the volume of the blood collected in the first syringe 10, in order to secure extraction concentration and recovery rate of PRP as much as possible.
Keeping the conical head of the first syringe 10 downward, adjusting the three-way valve to communicate the first syringe 10 with the second syringe 13, pushing the core rod of the first syringe 10 downward, and transferring the PRP layer in the first syringe 10 into the second syringe 13, so that only the PRP layer is contained in the second syringe 13 to extract the PRP. Preferably, the time taken for the first syringe 10 to transfer the PRP layer into the second syringe 13 exceeds 10 seconds, so as to avoid adhesion of the tunica albuginea layer after blood centrifugation to the red blood cells remaining on the vessel wall due to an excessively fast separation speed, thereby reducing interference on the PRP layer, stably performing extraction of PRP, and further ensuring stable platelets and recovery rate in PRP. Subsequently, the second syringe 13 is detached from the three-way valve and the sheath is fitted at the conical head.
Illustratively, the inventors have tested that in the case of 10 ml of blood collected by the first syringe 10 under the same centrifugation conditions, the first syringe 10 retains 0.3 ml to 1 ml of red blood cells transferred with the PRP layer into the second syringe 13, and the PRP transferred from the first syringe 10 into the second syringe 13 is 1 ml to 2 ml. In the case of 20 ml of blood collected through the first syringe 10, 0.6 ml to 2 ml of red blood cells retained by the first syringe 10 are transferred to the second syringe 13 along with the PRP layer, and the PRP transferred from the first syringe 10 to the second syringe 13 is 2 ml to 4 ml. In the case of collecting 30 ml of blood through the first syringe 10, the first syringe 10 retains 1 ml to 3 ml of red blood cells and the PRP layer is transferred into the second syringe 13, and the PRP transferred from the first syringe 10 into the second syringe 13 is 3 ml to 6 ml. In the case of collecting 50 ml of blood through the first syringe 10, 1.5 ml to 5 ml of red blood cells retained by the first syringe 10 are transferred to the second syringe 13 along with the PRP layer, and the PRP transferred from the first syringe 10 to the second syringe 13 is 5 ml to 10 ml. In the case of collecting 100 ml of blood through the first syringe 10, the first syringe 10 retains 3 ml to 10 ml of red blood cells and the PRP layer is transferred into the second syringe 13, and the PRP transferred from the first syringe 10 into the second syringe 13 is 10 ml to 20 ml.
In one specific example, the first syringe 10 collects 30 milliliters of blood and is centrifuged at a relative centrifugal force of 2200g for 20 minutes. 1 ml to 3 ml of red blood cells are retained in the first syringe 10 and transferred with the PRP layer into the second syringe 13. the PRP transferred from the first syringe 10 into the second syringe 13 may be 3 ml to 6 ml. In the case where the PRP extracted in the second syringe 13 was 5 ml, the average values of the platelet count in 30 ml of blood in the first syringe 10, the platelet count in 5 ml of PRP transferred to the second syringe 13, the platelet count in 19 ml of PPP left in the first syringe 10, and the platelet count in 5.8 ml of red blood cells transferred to the collection container 12 were (602 ± 54) × 10, respectively, as observed by an optical microscope9size/L, (2903 +/-91) × 109Per liter, (130 +/-1) multiplied by 109Per liter, (69 +/-1) multiplied by 109And (2) per liter. The ratio of the platelet count of PRP to that of blood was calculated to obtain an enrichment factor, i.e., (2903X 10)9)/(602×109) 4.80. In addition, the actual number of platelets in PRP, i.e., 5X 10, was calculated from the platelet count and PRP volume of PRP-3×2903×109And (4) respectively. The actual number of platelets in the blood, i.e. 30 x 10, was calculated from the platelet count and blood volume of the blood-3×602×109And (4) respectively. Finally, the recovery of platelets, i.e., (5X 2903X 10) is obtained from the actual number of platelets in the PRP and the actual number of platelets in the blood9)/(30×602×109) 80.4%. In conclusion, the method according to the present invention can adjust the final platelet enrichment factor by controlling the volume of the first syringe 10 transferred into the second syringe 13, and the extracted PRP has high platelet recovery rate and good extraction effect.
Furthermore, the time required to prepare PRP using a prior art apparatus for extracting PRP using a single centrifugation technique generally takes 15 minutes, and accordingly the platelet enrichment factor is approximately determined at 5 and the platelet recovery rate is approximately 60% or 62%. The time for preparing PRP using the apparatus for extracting PRP by the double centrifugation technique usually takes 20 minutes, and accordingly the platelet enrichment factor is roughly determined to be 6.40 ± 1.06 or 7.6 ± 1.2 and the platelet recovery rate is roughly (60.85 ± 8.97)% or (80.3 ± 12.8)%. In contrast, the device of the present invention for PRP preparation uses a single centrifugation technique, typically 15 minutes for preparation time, adjustable enrichment factor in the range of 3 to 10, and approximately (82 ± 14.5)%.
The thrombin is prepared by drawing a physiological saline solution using the third syringe 14, mounting a sheath on the tip of the third syringe 14, gently shaking the third syringe 14, and mixing the physiological saline solution and thrombin in the third syringe 14 to obtain the coagulant. The conical heads of the second syringe 13 containing the PRP and the third syringe 14 containing the coagulant are placed side by side with the conical heads facing down on the support frame 151, and a push plate 152 is attached to the head of the core rod of the second syringe 13 and the third syringe 14. The sheaths at the conical heads of the second injector 13 and the third injector 14 are detached, two liquid inlets of the spraying tee are respectively connected to the conical heads of the second injector 13 and the third injector 14 through luer connectors, and a nozzle with a built-in spraying head is connected to a liquid outlet of the spraying tee. The operator may then push the push plate 152 downward to push the second syringe 13 and the third syringe 14 core pins, and the PRP in the second syringe 13 and the coagulant in the third syringe 14 are pushed into the nozzle where they mix to produce a PRP gel, which is finally atomized by the spray head.
It should be understood that any combination of the operation steps of the extraction device in this embodiment to extract PRP should fall within the protection scope of the present invention.
It should be understood that although the description is in terms of various embodiments, not every embodiment includes only a single embodiment, and such description is for clarity purposes only, and those skilled in the art will recognize that the embodiments described herein may be combined as suitable to form other embodiments, as will be appreciated by those skilled in the art.
The above description is only an exemplary embodiment of the present invention, and is not intended to limit the scope of the present invention. Any equivalent alterations, modifications and combinations can be made by those skilled in the art without departing from the spirit and principles of the invention.
Claims (15)
1. A method of extracting platelet rich plasma from blood, the method comprising:
the conical head of a first syringe (10) containing anticoagulated blood is downward, the first syringe (10) is centrifuged for 5 to 45 minutes at a relative centrifugal force of 300 to 3000g, and the content in the first syringe (10) is layered into a platelet poor plasma layer, a platelet rich plasma layer and a red blood cell layer from top to bottom;
holding the first syringe (10) with its conical head facing downwards and connecting the first syringe (10) in fluid communication to a collection container (12) via a communication member (11), so that the layer of red blood cells in the first syringe (10) is transferred into the collection container (12) via the communication member (11);
-keeping the conical head of the first syringe (10) facing downwards and connecting the first syringe (10) in fluid communication via the communication member (11) to a second syringe (13), so that the platelet rich plasma layer inside the first syringe (10) is transferred into the second syringe (13) via the communication member (11) so that the second syringe (13) contains only the platelet rich plasma layer inside;
connecting in fluid communication said second syringe (13) containing platelet rich plasma and a third syringe (14) containing a coagulant to a spraying device (15);
and transferring the platelet-rich plasma in the second syringe (13) and the coagulant in the third syringe (14) to the spraying device (15), mixing the platelet-rich plasma and the coagulant in the spraying device (15) to generate platelet-rich gel, and spraying the platelet-rich gel from the spraying device (15).
2. The method according to claim 1, wherein the anticoagulated blood is obtained by:
drawing anticoagulant using the first syringe (10);
collecting blood using the first syringe (10), the ratio between anticoagulant drawn by the first syringe (10) and collected blood being 1: 9;
mixing the anticoagulant and the blood in the first syringe (10) to obtain anticoagulated blood.
3. The method according to claim 2, wherein the volume of blood collected by the first syringe (10) ranges from 10 ml to 100 ml.
4. The method of claim 1, wherein the first syringe (10) is centrifuged at a relative centrifugal force of 500g to 1500g for 15 minutes to 45 minutes.
5. The method according to claim 1, characterized in that the first syringe (10) is centrifuged at a relative centrifugal force of 1501g to 2500g for 10 to 30 minutes.
6. The method of claim 1, wherein the first syringe (10) is centrifuged at a relative centrifugal force of 2501g to 3500g for 5 minutes to 15 minutes.
7. The method of claim 1, wherein the first syringe (10) transfers the layer of contained red blood cells into the collection container (12) at a rate of no more than 0.5 milliliters per second.
8. The method of claim 1, wherein the first syringe (10) transfers only a portion of the layer of red blood cells to the collection container (12), leaving a remaining portion of the layer of red blood cells within the first syringe (10).
9. The method according to claim 8, characterized in that the volume of red blood cells left in the first syringe (10) ranges from 3% to 10% of the volume of blood collected.
10. The method according to claim 1, characterized in that the time required for the first syringe (10) to transfer the platelet rich plasma layer into the second syringe (13) exceeds 10 seconds.
11. The method of claim 1, wherein the coagulant is obtained by:
withdrawing saline using the third syringe (14);
thrombin is prepared using the third syringe (14), and the thrombin and the saline in the third syringe (14) are mixed to obtain the coagulant.
12. A device for extracting platelet rich plasma from blood for performing the method of any one of claims 1 to 11, the device comprising:
a first syringe (10) containing anticoagulated blood and centrifuging the anticoagulated blood for 5 to 45 minutes at a relative centrifugal force of 300 to 3000g in a state that a conical head is downward, wherein the content in the first syringe (10) is layered into a platelet poor plasma layer, a platelet rich plasma layer and a red blood cell layer from top to bottom;
a communication piece (11), wherein the first injector (10) is detachably connected to the communication piece (11) in a conical head-down posture;
a collection container (12) detachably connected to the communication member (11) so that the first syringe (10) and the collection container (12) are communicated via the communication member (11), and the erythrocyte layer in the first syringe (10) is transferred into the collection container (12) via the communication member (11);
a second syringe (13) detachably connected to the communication member (11) so that the platelet rich plasma layer in the first syringe (10) is transferred into the second syringe (13) through the communication member (11) to extract the platelet rich plasma from the second syringe (13) only containing the platelet rich plasma layer.
A spraying device (15), wherein the second syringe (13) containing platelet-rich plasma is detachably connected to the spraying device (15) after being detached from the communicating piece (11);
and a third syringe (14) which contains a coagulant and is detachably connected with the spraying device (15) so that the second syringe (13), the third syringe (14) and the spraying device (15) are in fluid communication, and the platelet-rich plasma in the second syringe (13) and the coagulant in the third syringe (14) can be transferred to the spraying device (15) and mixed in the spraying device (15) to generate platelet-rich gel and then are sprayed out.
13. Device according to claim 12, characterized in that the conical heads of the first syringe (10), the collection container (12), the second syringe (13) and the third syringe (14) are each detachably connected with a sheath.
14. Device according to claim 12, characterized in that the communication means (11) is a two-way valve or a three-way valve.
15. The device according to claim 12, characterized in that said spraying means (15) comprise:
a holding frame (151) in which the second syringe (13) and the third syringe (14) are held juxtaposed to each other;
a push plate (152) mounted to the heads of the second syringe (13) and the third syringe (14) core rod;
the spray tee joint comprises two liquid inlets and one liquid outlet, and the two liquid inlets of the spray tee joint are respectively connected to conical heads of the second injector (13) and the third injector (14);
a nozzle connected to the outlet of the spray tee to communicate the second syringe (13) and the third syringe (14) with the nozzle, wherein the platelet rich in the second syringe (13) and the coagulant in the third syringe (14) are transferred into the nozzle to mix to produce a gel in the nozzle;
and the spray head is arranged in the nozzle to spray the atomized gel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111043303.8A CN113828426A (en) | 2021-09-07 | 2021-09-07 | Method and device for extracting platelet-rich plasma from blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111043303.8A CN113828426A (en) | 2021-09-07 | 2021-09-07 | Method and device for extracting platelet-rich plasma from blood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113828426A true CN113828426A (en) | 2021-12-24 |
Family
ID=78958527
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111043303.8A Pending CN113828426A (en) | 2021-09-07 | 2021-09-07 | Method and device for extracting platelet-rich plasma from blood |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113828426A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115350509A (en) * | 2022-09-02 | 2022-11-18 | 杭州锐健马斯汀医疗器材有限公司 | Separating device |
| CN115475705A (en) * | 2022-09-01 | 2022-12-16 | 上海利格泰医用设备有限公司 | Using method of PRP making bag |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020175114A1 (en) * | 2001-04-05 | 2002-11-28 | Shintaro Nishimura | Supernatant liquid collector with balance weight forming function and centrifugal apparatus using the same |
| CN204073509U (en) * | 2014-09-11 | 2015-01-07 | 北京上惠好医疗器械有限公司 | For the preparation of the separating gun of rich platelet gel |
| CN106727700A (en) * | 2016-11-29 | 2017-05-31 | 第五空间健康管理江苏有限公司 | Prepare the method for platelet rich plasma PRP and the purposes of the platelet rich plasma |
| CN108554655A (en) * | 2018-01-03 | 2018-09-21 | 赛诺菲生物科技（武汉）有限公司 | Platelet rich plasma separator and separation method |
| CN110251995A (en) * | 2019-06-17 | 2019-09-20 | 郭钦 | A kind of economic preparation method of platelet rich plasma |
| CN210131413U (en) * | 2019-05-17 | 2020-03-10 | 广东省人民医院(广东省医学科学院) | Platelet-rich plasma separator |
| CN111172108A (en) * | 2019-11-26 | 2020-05-19 | 青岛大学 | Preparation method of tissue fluid |
| CN112675577A (en) * | 2019-10-17 | 2021-04-20 | 康膝生物医疗(深圳)有限公司 | Extraction device and method for extracting platelet-rich plasma from blood |
-
2021
- 2021-09-07 CN CN202111043303.8A patent/CN113828426A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020175114A1 (en) * | 2001-04-05 | 2002-11-28 | Shintaro Nishimura | Supernatant liquid collector with balance weight forming function and centrifugal apparatus using the same |
| CN204073509U (en) * | 2014-09-11 | 2015-01-07 | 北京上惠好医疗器械有限公司 | For the preparation of the separating gun of rich platelet gel |
| CN106727700A (en) * | 2016-11-29 | 2017-05-31 | 第五空间健康管理江苏有限公司 | Prepare the method for platelet rich plasma PRP and the purposes of the platelet rich plasma |
| CN108554655A (en) * | 2018-01-03 | 2018-09-21 | 赛诺菲生物科技（武汉）有限公司 | Platelet rich plasma separator and separation method |
| CN210131413U (en) * | 2019-05-17 | 2020-03-10 | 广东省人民医院(广东省医学科学院) | Platelet-rich plasma separator |
| CN110251995A (en) * | 2019-06-17 | 2019-09-20 | 郭钦 | A kind of economic preparation method of platelet rich plasma |
| CN112675577A (en) * | 2019-10-17 | 2021-04-20 | 康膝生物医疗(深圳)有限公司 | Extraction device and method for extracting platelet-rich plasma from blood |
| CN111172108A (en) * | 2019-11-26 | 2020-05-19 | 青岛大学 | Preparation method of tissue fluid |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115475705A (en) * | 2022-09-01 | 2022-12-16 | 上海利格泰医用设备有限公司 | Using method of PRP making bag |
| CN115350509A (en) * | 2022-09-02 | 2022-11-18 | 杭州锐健马斯汀医疗器材有限公司 | Separating device |
| CN115350509B (en) * | 2022-09-02 | 2023-11-24 | 杭州锐健马斯汀医疗器材有限公司 | separation device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4915847A (en) | Cryoglobulin separation | |
| EP3121260A1 (en) | Apparatus and methods for processing biological material | |
| US20110301545A1 (en) | Method of Using a Syringe | |
| CN113828426A (en) | Method and device for extracting platelet-rich plasma from blood | |
| CN102395392A (en) | System and method for the re-anticoagulation of platelet rich plasma | |
| CN103702729A (en) | Apparatus and method for separating and concentrating fluids containing multiple components | |
| CN108654141B (en) | Platelet-rich plasma preparation device | |
| CN216137429U (en) | Device for extracting platelet-rich plasma from blood | |
| US4917804A (en) | Method and vessel for separation of cryoglobin | |
| CN108295323A (en) | The preparation method of rich platelet gel | |
| WO2023086122A1 (en) | Connector for creating platelet rich plasma | |
| CN209422595U (en) | Detachable piston type acquisition separation injecting medicinal platelet rich plasma preparation facilities | |
| CN209772422U (en) | Integrated regeneration factor separation and enrichment preparation device | |
| EP0335882A1 (en) | Improved cryoglobulin separation | |
| CN208809066U (en) | Blood sampling with rich platelet gel preparation function joins bag | |
| CN1583213A (en) | Apparatus for preparing thrombocyte enriched blood plasma and preparing method thereof | |
| EP2982374A1 (en) | Red blood cell storage solution, storage solution accommodating container, manufacturing method for red blood cell storage solution, and red blood cell bag system | |
| CN210384934U (en) | Preparation facilities of rich platelet plasma | |
| EP3302397B1 (en) | Syringe for cell isolation | |
| CN209060173U (en) | A kind of infusion apparatus of included triple valve | |
| CN221638443U (en) | Platelet-rich plasma preparation device | |
| CN216703068U (en) | Combined and detachable platelet-rich plasma preparation device | |
| RU2271834C1 (en) | Device for carrying apparatus-free membrane plasmapheresis according to single-needle scheme | |
| CN214077269U (en) | PRP extraction element | |
| CN221814946U (en) | Double-tube injector |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |