CN113150982A - Novel cell biotechnology, kit and preparation device - Google Patents
Novel cell biotechnology, kit and preparation device Download PDFInfo
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Abstract
The invention discloses a novel cell biological culture technical kit, which comprises a sealed box body, a test tube bearing plate, a test tube limiting cylinder and a rotary pull ring, wherein the outer ring of the test tube bearing plate is in threaded connection with the bottom of the inner wall of the sealed box body, the bottom of the test tube limiting cylinder is fixedly connected with the top of the test tube bearing plate through a screw, the top of the rotary pull ring is fixedly welded with the bottom of the test tube bearing plate, and the action of a variable adding mechanism is used for realizing the automatic quantitative and synchronous adding of nutrients to a plurality of groups of cultures in the same environment under the condition of no human interference without excessive human participation, so that the uniformity of an experimental environment is ensured, when the nutrients are added each time, all the nutrients are added synchronously in the same amount, the comparison condition of a control group can be better realized, the accuracy of experimental data is ensured, and the automatic adding mode is adopted, the manual operation is reduced, and the culture experiment is more efficient for large-scale culture experiments.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a novel cell biotechnology, a kit and a preparation device.
Background
At present, in the field of biotechnology, the detection of biotechnology is one of the most important links, and biological reagents are necessary basic devices, biological reagents refer to biological materials or organic compounds related to life science research, and reagents for clinical diagnosis and medical research. Because of wide range of life science and fast development, the reagent has various varieties and complex properties. The reagent kit mainly comprises electrophoresis reagents, chromatographic reagents, centrifugal separation reagents, immunological reagents, labeling reagents, histochemical reagents, transmutation reagents and carcinogenic substances, pesticides, culture agents, buffering agents, electron microscope reagents, protein and nucleic acid precipitating agents, condensing agents, ultrafiltration membranes, clinical diagnosis reagents, coloring agents, antioxidants, mildew inhibitors, detergents and surfactants, biochemical standard reagents, biochemical quality control reagents, separation materials and the like, wherein solid reagents are generally arranged in wide-mouth bottles with bakelite stoppers, liquid reagents are contained in narrow-mouth bottles, visible light easily decomposed reagents are arranged in brown bottles, each reagent is labeled to indicate the name, concentration and purity of the reagent, and the current operation method is arranged in the reagent kit for detection.
In the process of biological cell culture, not only a group of experimental samples are cultured, but in order to meet scientificity, a large number of experimental samples are usually adopted for simultaneous culture, a biological culture technology is embodied by big data, but in the culture process, an optimal culture condition and an optimal culture environment need to be obtained through experiments, therefore, a plurality of variables are usually required to be set under the same environment for experimental observation, so that the condition which is more suitable for biological cell culture is obtained, however, under the same environment condition, unique variable control is extremely difficult, manual operation cannot avoid interference of the external environment, reliability of experimental data is undoubtedly reduced, and the addition of the variables is controlled, single manual operation is also difficult to carry out, therefore, under the same environment and without increasing the interference of the external environment, the variable control and accurate addition are a great problem to be solved in biological cell tests.
Disclosure of Invention
The present invention is directed to a novel cell biotechnology, a kit and a preparation apparatus, which solve the problems of the background art mentioned above.
In order to achieve the purpose, the invention provides the following technical scheme: a novel cell biotechnology, comprising the steps of:
the method comprises the following steps: in the experiment preparation stage, living tissues and cells suitable for in vitro culture are selected for later use through medical equipment and instruments, such as a cell sampling device, a culture dish, a liquid medicine test tube and the like, a plurality of experimental culture kits are selected for later use, a culture medium for tissue culture is configured, and a plurality of different nutrient additives are prepared for variable comparison;
step two: in the culture experiment stage, the living tissues obtained in the step one are planted in the culture medium prepared in the step one, the number of the living tissues is required to be prepared according to the number of variables in proper parts, then the prepared tissue culture is placed in a kit, and finally, different variable nutrients are continuously added in real time through a preparation device in the same environment;
step three: in the experimental data collection stage, according to a series of operations in the second step, the culture state of the living tissue in a plurality of different test tubes is monitored and various index data are recorded;
step four: and (4) data arrangement and analysis, namely, according to the growth conditions recorded in the step three and the analysis and comparison of all data, a corresponding conclusion is drawn, namely, the most suitable cell biological culture is carried out under the same growth environment and the influence of different nutrients on the production of living tissues.
The utility model provides a novel cell biotechnology kit, includes sealed box body, test tube loading board, the spacing section of thick bamboo of test tube and rotatory pull ring, the outer lane of test tube loading board and the bottom threaded connection of sealed box body inner wall, the top fixed connection of screw and test tube loading board is passed through to the bottom of the spacing section of thick bamboo of test tube, the top of rotatory pull ring and the bottom fixed weld of test tube loading board.
Preferably, the novel cell biotechnology preparation device comprises a reagent cylinder and a variable adding mechanism, wherein the bottom of the reagent cylinder is detachably connected with the top of the sealing box body through a buckle, and the variable adding mechanism is arranged at the top of the reagent cylinder.
Preferably, a plurality of containing cylinders are installed at the bottom of the inner cavity of the reagent cylinder through screws, and brackets are fixedly welded at the top of the reagent cylinder at equal intervals.
Preferably, the variable adds the mechanism and includes positioning disk, gasket, take-up (stock) pan, linkage post, step motor, maintenance spring, connects material hole, carry pull ring and locating lever, the outer wall of positioning disk passes through the inner wall fixed connection of bolt and sealed box body, the gasket passes through glue and bonds at the top of positioning disk, the take-up (stock) pan overlap joint is at the top of gasket, bolt and take-up (stock) pan fixed connection are passed through to the bottom of linkage post, step motor passes through the top fixed connection of shaft coupling and linkage post, maintenance spring suit is on the outer lane of linkage post, connect the material hole to set up at the top of take-up (stock) pan, carry the bottom of pull ring suit at linkage post outer lane, the top of locating lever and the bottom fixed weld of carrying pull ring.
Preferably, the bottom of maintenance spring and the top fixed weld of lift ring, the top of maintenance spring and the outer wall fixed weld of linkage post, the inner chamber that take-up dish and extend to the take-up dish is run through to the bottom of locating lever.
Preferably, the top of positioning disk has been seted up and has been hidden the groove, the movable joint of the inner chamber of hiding the groove has the activity arc, the dead slot has been seted up to the side of activity arc, the inner chamber of dead slot is provided with reset spring, the fixed welding in front of activity arc has the magnetic path, one section fixed welding that dries in the air of activity arc has the air compression piece, the material leaking hole has been seted up to the one end of hiding the inslot chamber, the inner chamber threaded connection in material leaking hole has the weeping head.
Compared with the prior art, the invention has the beneficial effects that:
1. this neotype cell biotechnology, kit and preparation facilities adopt the sealed mode of settling the cultivation of test tube isotructure of kit and the biological living organism tissue of splendid attire to carry out the cell culture with the mode that the multiunit was cultivateed simultaneously, can realize the purpose that a large amount of experimental samples cultivateed under the same environment fast, distinguish again with the multiunit in every a set of simultaneously, and add different nutritional reagents, ensured the contrast of the control of realizing different variables under same environment.
2. This neotype cell biotechnology, kit and preparation facilities, with the action of variable interpolation mechanism, realize that the automatic ration of the condition lower door of no human interference carries out the synchronous interpolation nutrient of ration for being in multiunit culture in same environment, on the one hand can add automatically, need not artificial too much participation, experimental environment's uniformity has been guaranteed, on the other hand is when adding the nutrient at every turn, all be synchronous with the interpolation of appearance volume, the contrast condition of realization contrast group that like this can be better, ensure test data's accuracy, and with the mode of automatic interpolation, reduce manual operation, to the culture experiment of large-scale quantity, it is more efficient.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the structure of the variable adding mechanism of the present invention;
FIG. 3 is a schematic view of the receiver tray of the present invention;
FIG. 4 is an enlarged view of the invention at A in FIG. 3.
In the figure: 1 sealed box body, 2 reagent tubes, 201 containing tube, 202 brackets, 3 variable adding mechanisms, 301 positioning disc, 302 sealing plate, 303 receiving disc, 3011 hidden groove, 3012 movable arc, 3013 empty groove, 3014 reset spring, 3015 magnet, 3016 air compression block, 3017 leakage hole, 3018 leakage head, 304 linkage column, 305 stepping motor, 306 maintenance spring, 307 receiving hole, 308 lifting ring, 309 positioning rod, 4 test tube bearing plate, 5 test tube limiting tube and 6 rotary pulling ring.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution:
a novel cell biotechnology, comprising the steps of:
the method comprises the following steps: in the experiment preparation stage, living tissues and cells suitable for in vitro culture are selected for later use through medical equipment and instruments, such as a cell sampling device, a culture dish, a liquid medicine test tube and the like, a plurality of experimental culture kits are selected for later use, a culture medium for tissue culture is configured, and a plurality of different nutrient additives are prepared for variable comparison;
step two: in the culture experiment stage, the living tissues obtained in the step one are planted in the culture medium prepared in the step one, the number of the living tissues is required to be prepared according to the number of variables in proper parts, then the prepared tissue culture is placed in a kit, and finally, different variable nutrients are continuously added in real time through a preparation device in the same environment;
step three: in the experimental data collection stage, according to a series of operations in the second step, the culture state of the living tissue in a plurality of different test tubes is monitored and various index data are recorded;
step four: and (4) data arrangement and analysis, namely, according to the growth conditions recorded in the step three and the analysis and comparison of all data, a corresponding conclusion is drawn, namely, the most suitable cell biological culture is carried out under the same growth environment and the influence of different nutrients on the production of living tissues.
The utility model provides a novel cell biotechnology kit, including sealed box body 1, test tube loading board 4, the spacing section of thick bamboo of test tube 5 and rotatory pull ring 6, the outer lane of test tube loading board 4 and the bottom threaded connection of sealed box body 1 inner wall, the top fixed connection of screw and test tube loading board 4 is passed through to the bottom of the spacing section of thick bamboo of test tube 5, the top of rotatory pull ring 6 and the bottom fixed weld of test tube loading board 4, including reagent section of thick bamboo 2, add mechanism 3 with the variable, the bottom of reagent section of thick bamboo 2 is through the detachable connection in buckle and the top of sealed box body 1, variable adds mechanism 3 and sets up at the top of reagent section of thick bamboo 2, the bottom of reagent section of thick bamboo 2 inner chamber has a plurality of splendid attire section of thick bamboo 201 through the screw mounting, the top equidistance fixed welding of reagent section of thick bamboo 2 has bracket 202, variable adds mechanism 3 and includes positioning disk 301, gasket 302, take-up dish 303, linkage post 304, step motor 305, The inspection device comprises an inspection spring 306, a material receiving hole 307, a lifting ring 308 and a positioning rod 309, wherein the outer wall of a positioning disc 301 is fixedly connected with the inner wall of a sealing box body 1 through bolts, a sealing sheet 302 is adhered to the top of the positioning disc 301 through glue, the material receiving disc 303 is lapped on the top of the sealing sheet 302, the bottom of a linkage column 304 is fixedly connected with the material receiving disc 303 through bolts, a stepping motor 305 is fixedly connected with the top of the linkage column 304 through a coupler, the inspection spring 306 is sleeved on the outer ring of the linkage column 304, the material receiving hole 307 is formed in the top of the material receiving disc 303, the lifting ring 308 is sleeved on the bottom of the outer ring of the linkage column 304, the top of the positioning rod 309 is fixedly welded with the bottom of the lifting ring 308, the bottom of the inspection spring 306 is fixedly welded with the top of the lifting ring 308, the top of the inspection spring 306 is fixedly welded with the outer wall of the linkage column 304, the bottom of the positioning rod 309 penetrates through the material receiving disc 303 and extends to the inner cavity of the material receiving disc 303, the top of positioning disk 301 has been seted up and has been hidden groove 3011, and the movable joint of the inner chamber of hiding groove 3011 has activity arc 3012, and dead slot 3013 has been seted up to the side of activity arc 3012, and the inner chamber of dead slot 3013 is provided with reset spring 3014, and the fixed welding in front of activity arc 3012 has magnetic path 3015, and one section fixed welding that dries in the air of activity arc 3012 has air compression piece 3016, and the one end of hiding the inner chamber of groove 3011 has been seted up hourglass material hole 3017, and the inner chamber threaded connection of hourglass material hole 3017 has weeping head 3018.
The working principle is as follows: the method comprises the following steps: in the experiment preparation stage, living tissues and cells suitable for in vitro culture are selected through medical equipment and instruments for later use, such as a cell sampling device, a culture dish, a liquid medicine test tube and the like, a plurality of experimental culture kits are selected for later use, a culture medium for tissue culture is configured, a plurality of different nutrient additives are prepared for variable comparison, and the step two is as follows: in the culture experiment stage, the living tissue obtained in the step one is planted in the culture medium prepared in the step one, the quantity of the living tissue needs to be prepared according to the number of variables in proper parts, then the prepared tissue culture is placed into a kit, and finally, the addition of different variable nutrients is carried out in real time and uninterruptedly in the same environment through a preparation device, and the step three is as follows: and in the experimental data collection stage, according to a series of operations in the second step, monitoring the culture state of the living tissues in a plurality of different test tubes and recording various index data, and in the fourth step: data arrangement and analysis, according to the growth conditions recorded in the third step and the analysis and comparison of each data, a corresponding conclusion is obtained, namely, in the same growth environment, the influence of different nutrients on the production of living tissues is most suitable for carrying out cell biological culture, wherein in the link of a culture selection test, the process is carried out in a full-automatic mode, namely, variable culture solution to be added is placed in the inner cavity of the containing cylinder 201 in advance, the outlet at the bottom of the containing cylinder 201 is tangent and sealed with the receiving disc 303, the culture solution is contacted with the receiving disc 303, when the stepping motor 305 drives the receiving disc 303 to rotate, the receiving hole 307 on the receiving disc is filled with the culture solution and then continues to rotate, when the receiving hole 307 is aligned with the leakage hole 3017, the culture solution enters the leakage hole 3017, then under the pushing of the magnetic adding block at the bottom of the receiving disc, the magnetic block 3015 is driven to continuously push the air compression block 3016 to move in the inner cavity of the hidden slot 3011, along with the continuous movement of the air compression block 3016, the culture solution remained in the inner cavity of the material leakage hole 3017 is extruded into the test tube at the bottom, wherein the material leakage holes 3017, the hidden slot 3011 and the material receiving hole 307 are staggered and do not interfere with each other.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A novel cell biological culture technology is characterized by comprising the following steps:
the method comprises the following steps: in the experiment preparation stage, living tissues and cells suitable for in vitro culture are selected for later use through medical equipment and instruments, such as a cell sampling device, a culture dish, a liquid medicine test tube and the like, a plurality of experimental culture kits are selected for later use, a culture medium for tissue culture is configured, and a plurality of different nutrient additives are prepared for variable comparison;
step two: in the culture experiment stage, the living tissues obtained in the step one are planted in the culture medium prepared in the step one, the number of the living tissues is required to be prepared according to the number of variables in proper parts, then the prepared tissue culture is placed in a kit, and finally, different variable nutrients are continuously added in real time through a preparation device in the same environment;
step three: in the experimental data collection stage, according to a series of operations in the second step, the culture state of the living tissue in a plurality of different test tubes is monitored and various index data are recorded;
step four: and (4) data arrangement and analysis, namely, according to the growth conditions recorded in the step three and the analysis and comparison of all data, a corresponding conclusion is drawn, namely, the most suitable cell biological culture is carried out under the same growth environment and the influence of different nutrients on the production of living tissues.
2. The utility model provides a novel cell biological culture technique kit, includes sealed box body (1), test tube loading board (4), the spacing section of thick bamboo of test tube (5) and rotatory pull ring (6), its characterized in that: the utility model discloses a test tube loading plate, including test tube loading plate (4), the bottom threaded connection of the outer lane of test tube loading plate (4) and sealed box body (1) inner wall, the top fixed connection of screw and test tube loading plate (4) is passed through to the bottom of the spacing section of thick bamboo of test tube (5), the top of rotatory pull ring (6) and the bottom fixed weld of test tube loading plate (4).
3. A novel cell biological culture technology preparation device comprises a reagent cylinder (2) and a variable adding mechanism (3), and is characterized in that: the bottom of the reagent cylinder (2) is detachably connected with the top of the sealed box body (1) through a buckle, and the variable adding mechanism (3) is arranged at the top of the reagent cylinder (2).
4. A novel device for preparing cell biological culture technology according to claim 3, characterized in that: a plurality of containing cylinders (201) are installed at the bottom of the inner cavity of the reagent cylinder (2) through screws, and brackets (202) are fixedly welded at the top of the reagent cylinder (2) at equal intervals.
5. The device for preparing cell biological culture technique according to claim 4, wherein: the variable adding mechanism (3) comprises a positioning disc (301), a sealing sheet (302), a material receiving disc (303), a linkage column (304), a stepping motor (305), an overhauling spring (306), a material receiving hole (307), a lifting ring (308) and a positioning rod (309), wherein the outer wall of the positioning disc (301) is fixedly connected with the inner wall of the sealing box body (1) through a bolt, the sealing sheet (302) is adhered to the top of the positioning disc (301) through glue, the material receiving disc (303) is lapped on the top of the sealing sheet (302), the bottom of the linkage column (304) is fixedly connected with the material receiving disc (303) through a bolt, the stepping motor (305) is fixedly connected with the top of the linkage column (304) through a coupler, the overhauling spring (306) is sleeved on the outer ring of the linkage column (304), the material receiving hole (307) is formed in the top of the material receiving disc (303), and the lifting ring (308) is sleeved on the bottom of the linkage column (304), the top of the positioning rod (309) is fixedly welded with the bottom of the lifting ring (308).
6. The device for preparing cell biological culture technique according to claim 5, wherein: the bottom of maintenance spring (306) and the top fixed weld of lift-tab (308), the top of maintenance spring (306) and the outer wall fixed weld of linkage post (304), the inner chamber that take-up dish (303) and extend to take-up dish (303) is run through to the bottom of locating lever (309).
7. The device for preparing cell biological culture technique according to claim 5, wherein: hidden groove (3011) have been seted up at the top of positioning disk (301), the inner chamber activity joint of hiding groove (3011) has activity arc (3012), dead slot (3013) have been seted up to the side of activity arc (3012), the inner chamber of dead slot (3013) is provided with reset spring (3014), the positive fixed welding of activity arc (3012) has magnetic path (3015), one section fixed welding that dries in the air of activity arc (3012) has air compression piece (3016), material leakage hole (3017) have been seted up to the one end of hiding groove (3011) inner chamber, the inner chamber threaded connection of material leakage hole (3017) has liquid leakage head (3018).
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| CN202110158399.6A CN113150982A (en) | 2021-02-04 | 2021-02-04 | Novel cell biotechnology, kit and preparation device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115508404A (en) * | 2022-10-24 | 2022-12-23 | 重庆大学 | Single-variable control test method for field natural icing |
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