CN108251303A - A kind of kit for being used for candidate stem cell in vitro culture and differentiation - Google Patents
A kind of kit for being used for candidate stem cell in vitro culture and differentiation Download PDFInfo
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- CN108251303A CN108251303A CN201810247990.7A CN201810247990A CN108251303A CN 108251303 A CN108251303 A CN 108251303A CN 201810247990 A CN201810247990 A CN 201810247990A CN 108251303 A CN108251303 A CN 108251303A
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- carrier
- stem cell
- candidate stem
- culture dish
- carrier box
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/24—Draft tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
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- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
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Abstract
The present invention relates to a kind of for candidate stem cell in vitro culture and the kit of differentiation, including Carrier box, sealing cover, carrier, culture dish, reagent bottle, metering pump, diversion pipe, isocon, register and control circuit, it is hinged between Carrier box and sealing cover, carrier is in Carrier box, several positioning chambers are set in carrier, in bearing cavity, metering pump is mounted on positioning chamber for culture dish and reagent bottle.One aspect of the present invention can effectively improve the flexibility adjusted to candidate stem cell training environment and operation stability, and the needs for carrying out multiple types to candidate stem cell and synchronizing culture operation can be met, on the other hand the control accuracy of the flexibility of reagent addition operation and reagent addition operation in candidate stem cell incubation can be effectively improved, and can effectively avoid the cultivation temperature shakiness easily led to during reagent addition and occurred by external environment contamination phenomenon.
Description
Technical field
The present invention relates to a kind of candidate stem cell culture devices, are exactly that one kind is used for candidate stem cell in vitro culture
With the kit of differentiation.
Background technology
At present into during candidate stem cell culture, culture dish is placed on to the culture box for having ambient stable when current main
Or carried out in incubator, although the needs to candidate stem cell culture operation can be substantially met, find in actual use,
Current used culture device often there are device structure complexity, using operating flexibility it is poor the defects of, it is while also different
Degree exists in candidate stem cell incubation, and into culture dish, addition reagent task difficulty is big, and in reagent adding procedure
In easily lead to culture device running temperature and be a greater impact, while also easily lead to culture dish and sent out by external environment contamination phenomenon
Raw, simultaneously, when carrying out candidate stem cell culture operation, an equipment is often only capable of meeting same type current equipment
The needs of candidate stem cell culture experiment operation, therefore inefficient operation, it is impossible to while meet a variety of cultures of candidate stem cell
The needs of operation are tested, have seriously affected flexibility and reliability that candidate stem cell culture and experiment are done, therefore for this
Present situation, there is an urgent need to develop a kind of completely new candidate stem cell culture device, to meet the needs of actual use.
Invention content
In view of the shortcomings of the prior art, the present invention provides a kind of simple in structure, flexible and convenient to use and versatility
The good kit for being used for candidate stem cell in vitro culture and differentiation, can effectively improve to candidate stem cell training environment tune
The flexibility of section and operation stability, and the needs for carrying out multiple types to candidate stem cell and synchronizing culture operation can be met, it carries
The flexibility of high candidate stem cell culture operation and efficiency, on the other hand can effectively improve reagent in candidate stem cell incubation
The flexibility of operation and the control accuracy of reagent addition operation are added, and can effectively avoid the culture temperature easily led to during reagent addition
Degree is unstable and is occurred by external environment contamination phenomenon, so as to greatly improve the efficiency and matter of candidate stem cell culture operation
Amount.
To achieve these goals, the present invention is to realize by the following technical solutions：
It is a kind of for candidate stem cell in vitro culture and differentiation kit, including Carrier box, sealing cover, carrier, culture dish,
Reagent bottle, metering pump, diversion pipe, isocon, register and control circuit, are hinged between Carrier box and sealing cover, and structure
The cavity body structure of the closed structure rectangular into cross section, carrier are coaxially distributed, carrier in Carrier box with Carrier box
It is mutually sliding by least two directive slide tracks between carrier and Carrier box medial surface for the frame structure being coaxially distributed with Carrier box
It is dynamic to connect, several partition boards are set in carrier, and pass through partition board to be divided at least two bearing cavitys and a positioning in carrier
Chamber, and positioning chamber is coaxially distributed with Carrier box, carrying cavity axis is evenly distributed with Carrier box axis parallel distribution and around positioning chamber, is trained
It supports ware and reagent bottle is at least one, each culture dish and reagent bottle are coaxially distributed in bearing cavity and with bearing cavity, are measured
Pump at least one, in positioning chamber, and metering pump one end is interconnected by diversion pipe and reagent bottle, another section by point
Flow tube is interconnected with culture dish, and the register is at least one, on Carrier box side wall and around carrier axle
Line is evenly distributed with, and control circuit is embedded in Carrier box lateral surface, and is electrically connected respectively with metering pump, register.
Further, it is equal between culture dish, reagent bottle and carrier between the carrier and Carrier box inner surface
It is connected with each other by least two electromagnetic location pins, and the electromagnetic location pin and Carrier box axis are in 45 ° -90 ° angles.
Further, the culture dish and reagent bottle are connected with each other with Carrier box bottom by cushion.
Further, the culture dish airtight cavity structure rectangular for axial cross section, and culture dish upper surface is set
At least one flow-guiding mouth, and pass through flow-guiding mouth and be interconnected with isocon, culture dish sidewall bottom sets at least one sewage draining exit.
Further, the register includes irradiated heat device, semiconductor refrigerating mechanism, and the irradiation adds
Thermal, semiconductor refrigerating mechanism equal at least two, spaced distribution between each irradiated heat device, semiconductor refrigerating mechanism.
Further, the diversion pipe, isocon are mutual by control valve and culture dish, reagent bottle, metering pump respectively
Connection, the control valve are electrically connected with control circuit.
Further, the control circuit is SCM Based control circuit, and control circuit sets at least one show
Show device and at least one manipulation keyboard.
The configuration of the present invention is simple, flexible and convenient to use, versatility is good, on the one hand can effectively improve to candidate stem cell culture
The flexibility and operation stability that working environment is adjusted, and can meet and multiple types synchronization culture operation is carried out to candidate stem cell
Needs, improve candidate stem cell culture operation flexibility and efficiency, on the other hand can effectively improve candidate stem cell culture
The control accuracy of the flexibility of reagent addition operation and reagent addition operation in the process, and can effectively avoid when reagent adds and easily lead
The cultivation temperature of cause is unstable and is occurred by external environment contamination phenomenon, so as to greatly improve candidate stem cell culture operation
Efficiency and quality.
Description of the drawings
The following describes the present invention in detail with reference to the accompanying drawings and specific embodiments.
Fig. 1 is schematic structural view of the invention；
Specific embodiment
To be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, with reference to specific
Embodiment, the present invention is further explained.
A kind of kit for being used for candidate stem cell in vitro culture and differentiation as described in Figure 1, including Carrier box 1, sealing
Lid 2, carrier 3, culture dish 4, reagent bottle 5, metering pump 6, diversion pipe 7, isocon 8, register 9 and control circuit 10, hold
It carries and is hinged, and form the cavity body structure of the rectangular closed structure in cross section between box 1 and sealing cover 2, carrier 3 is embedded in and holds
It carries in box 1, with the coaxial distribution of Carrier box 1, carrier 3 is the frame structure being coaxially distributed with Carrier box 1, carrier 3 and carrying
Mutually be slidably connected by least two directive slide tracks 11 between 1 medial surface of box, set several partition boards 12 in carrier 3, and by every
Plate 12 will be divided at least two bearing cavitys 13 and a positioning chamber 14 in carrier 3, and coaxial point of positioning chamber 14 and Carrier box 1
Cloth, 13 axis of bearing cavity is with 1 axis parallel distribution of Carrier box and uniformly distributed around positioning chamber 14, and culture dish 4 and reagent bottle 5 are at least
One, each culture dish 4 and reagent bottle 5 in bearing cavity 13 and with the coaxial distribution of bearing cavity 13, metering pump 6 is at least one,
In positioning chamber 14, and 6 one end of metering pump is interconnected by diversion pipe 7 and reagent bottle 5, and another section passes through isocon 8
It being interconnected with culture dish 4, register 9 is at least one, is evenly distributed on 1 side wall of Carrier box and around 3 axis of carrier,
Control circuit 10 is embedded in 1 lateral surface of Carrier box, and is electrically connected respectively with metering pump 6, register 9.
In the present embodiment, between 2 inner surface of the carrier 3 and Carrier box and culture dish 4, reagent bottle 5 and carrier 3
Between be connected with each other by least two electromagnetic location pins 15, and the electromagnetic location pin 15 is in Carrier box axis
45 ° -90 ° angles.
In the present embodiment, the culture dish 4 and reagent bottle 5 are connected with each other with 1 bottom of Carrier box by cushion 16.
In the present embodiment, the culture dish 4 airtight cavity structure rectangular for axial cross section, and 4 upper end of culture dish
Face sets at least one flow-guiding mouth 41, and passes through 41 mouthfuls of water conservancy diversion and be interconnected with isocon 7, and 4 sidewall bottom of culture dish sets at least one
A sewage draining exit 42.
In the present embodiment, the register 9 includes irradiated heat device, semiconductor refrigerating mechanism, and the spoke
According to heating unit, semiconductor refrigerating mechanism equal at least two, spaced point between each irradiated heat device, semiconductor refrigerating mechanism
Cloth.
In the present embodiment, the diversion pipe 7, isocon 8 pass through control valve 17 and culture dish 4, reagent bottle 5, meter respectively
Amount pump 6 is connected with each other, and the control valve 17 is electrically connected with control circuit 10.
In the present embodiment, the control circuit 10 is SCM Based control circuit, and control circuit sets at least one
A display and at least one manipulation keyboard.
The present invention in the specific implementation, first to Carrier box, sealing cover, carrier, metering pump, diversion pipe, isocon, tune
Warm device and control circuit assemble culture dish and reagent spare, that then will carry medium body and related reagent respectively
Bottle is installed to by carrier in Carrier box, and Carrier box is sealed, finally by control circuit driving register fortune
Temperature in Carrier box is adjusted to the needs of candidate stem cell culture operation by row.
Then it in incubation, is run by control circuit driving measuring pump and control valve, realizing will be in reagent bottle
Reagent according to use need positioning when it is quantitative be added in culture dish, meeting the same of candidate stem cell culture operation needs
When, separately reaching prevents from leading to the temperature fluctuation of candidate stem cell culture environment in reagent adding procedure and be polluted by external environment
Phenomenon occurs.
The configuration of the present invention is simple, flexible and convenient to use, versatility is good, on the one hand can effectively improve to candidate stem cell culture
The flexibility and operation stability that working environment is adjusted, and can meet and multiple types synchronization culture operation is carried out to candidate stem cell
Needs, improve candidate stem cell culture operation flexibility and efficiency, on the other hand can effectively improve candidate stem cell culture
The control accuracy of the flexibility of reagent addition operation and reagent addition operation in the process, and can effectively avoid when reagent adds and easily lead
The cultivation temperature of cause is unstable and is occurred by external environment contamination phenomenon, so as to greatly improve candidate stem cell culture operation
Efficiency and quality.
It should be understood by those skilled in the art that the present invention is not limited to the above embodiments.Above-described embodiment and explanation
Merely illustrating the principles of the invention described in book.Without departing from the spirit and scope of the present invention, the present invention also has
Various changes and modifications.These changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention
It is defined by the appending claims and its equivalent thereof.
Claims (7)
- It is 1. a kind of for candidate stem cell in vitro culture and the kit of differentiation, it is characterised in that：Described is thin for Hematopoietic Stem Born of the same parents' in vitro culture and differentiation kit include Carrier box, sealing cover, carrier, culture dish, reagent bottle, metering pump, diversion pipe, Isocon, register and control circuit are hinged, and it is rectangular to form cross section between the Carrier box and sealing cover The cavity body structure of closed structure, the carrier are coaxially distributed in Carrier box with Carrier box, and the carrier is with holding The frame structure that box is coaxially distributed is carried, it is mutually sliding by least two directive slide tracks between the carrier and Carrier box medial surface It is dynamic to connect, several partition boards are set in carrier, and pass through partition board to be divided at least two bearing cavitys and a positioning in carrier Chamber, and the positioning chamber is coaxially distributed with Carrier box, carrying cavity axis is with Carrier box axis parallel distribution and around positioning chamber Uniformly distributed, the culture dish and reagent bottle is at least one, and each culture dish and reagent bottle are in bearing cavity and and bearing cavity Coaxial distribution, the metering pump is at least one, and in positioning chamber, and metering pump one end passes through diversion pipe and reagent bottle phase Intercommunicated, another section is interconnected by isocon and culture dish, and the register is at least one, mounted on Carrier box side It is evenly distributed on wall and around carrier axis, the control circuit is embedded in Carrier box lateral surface, and is filled respectively with metering pump, temperature adjustment Put electrical connection.
- It is 2. according to claim 1 a kind of for candidate stem cell in vitro culture and the kit of differentiation, it is characterised in that： Determined between culture dish, reagent bottle and carrier by least two electromagnetism between the carrier and Carrier box inner surface Position pin is connected with each other, and the electromagnetic location pin and Carrier box axis are in 45 ° -90 ° angles.
- It is 3. according to claim 1 a kind of for candidate stem cell in vitro culture and the kit of differentiation, it is characterised in that： The culture dish and reagent bottle are connected with each other with Carrier box bottom by cushion.
- It is 4. according to claim 1 a kind of for candidate stem cell in vitro culture and the kit of differentiation, it is characterised in that： The culture dish airtight cavity structure rectangular for axial cross section, and culture dish upper surface sets at least one flow-guiding mouth, and It is interconnected by flow-guiding mouth and isocon, culture dish sidewall bottom sets at least one sewage draining exit.
- It is 5. according to claim 1 a kind of for candidate stem cell in vitro culture and the kit of differentiation, it is characterised in that： The register includes irradiated heat device, semiconductor refrigerating mechanism, and the irradiated heat device, semiconductor refrigerating Mechanism equal at least two, spaced distribution between each irradiated heat device, semiconductor refrigerating mechanism.
- It is 6. according to claim 1 a kind of for candidate stem cell in vitro culture and the kit of differentiation, it is characterised in that： The diversion pipe, isocon pass through control valve respectively and culture dish, reagent bottle, metering pump are connected with each other, the control valve It is electrically connected with control circuit.
- It is 7. according to claim 1 a kind of for candidate stem cell in vitro culture and the kit of differentiation, it is characterised in that： The control circuit is SCM Based control circuit, and control circuit sets at least one display and at least one manipulation Keyboard.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810247990.7A CN108251303A (en) | 2018-03-23 | 2018-03-23 | A kind of kit for being used for candidate stem cell in vitro culture and differentiation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810247990.7A CN108251303A (en) | 2018-03-23 | 2018-03-23 | A kind of kit for being used for candidate stem cell in vitro culture and differentiation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108251303A true CN108251303A (en) | 2018-07-06 |
Family
ID=62747461
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810247990.7A Withdrawn CN108251303A (en) | 2018-03-23 | 2018-03-23 | A kind of kit for being used for candidate stem cell in vitro culture and differentiation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108251303A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113150982A (en) * | 2021-02-04 | 2021-07-23 | 张仪 | Novel cell biotechnology, kit and preparation device |
-
2018
- 2018-03-23 CN CN201810247990.7A patent/CN108251303A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113150982A (en) * | 2021-02-04 | 2021-07-23 | 张仪 | Novel cell biotechnology, kit and preparation device |
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| PB01 | Publication | ||
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Application publication date: 20180706 |