CN112046934A

CN112046934A - Intelligent biological sample save set based on water-cooling ultra-low temperature

Info

Publication number: CN112046934A
Application number: CN202010910184.0A
Authority: CN
Inventors: 黄智�; 禹文峰; 王碧
Original assignee: Guizhou Medical University
Current assignee: Affiliated Hospital of Guizhou Medical University
Priority date: 2020-09-02
Filing date: 2020-09-02
Publication date: 2020-12-08

Abstract

The invention provides an intelligent biological sample storage device based on water-cooling ultralow temperature, which comprises a storage box, a water-cooling refrigeration device, an access window, a positioning access device and a touch control device, wherein the storage box comprises a storage part and an access part, the storage part comprises a storage box, a storage rack, a storage tank and a hook, the access part comprises a traction motor arranged corresponding to the hook, the water-cooling refrigeration device comprises a refrigeration compressor, a condenser and a refrigeration coil pipe, the access window comprises a storage tank, a scanner, an expansion bracket and an electric sealing cover, the positioning access device is arranged above the access part, and the positioning access device comprises a sliding track, a sliding cross rod, a sliding block, an electric lifting rod and a grabbing head. In a word, the invention has the advantages of novel structure, convenient taking, safety, high efficiency and the like.

Description

Intelligent biological sample save set based on water-cooling ultra-low temperature

Technical Field

The invention belongs to the technical field of sample preservation, and particularly relates to an intelligent biological sample preservation device based on water cooling and ultralow temperature.

Background

The biological sample bank is also called biological bank (Biobank) and mainly refers to standardized collection, processing, storage and application of samples such as biological macromolecules, cells, tissues and organs of healthy and disease organisms (including human organ tissues, whole blood, blood plasma, blood serum, biological body fluid or processed biological samples (DNA, RNA, protein and the like), and clinical, pathological, treatment, follow-up visit, informed consent and other data related to the biological samples, and a quality control, information management and application system thereof.

Biological samples are important resources of medical research, pathological tissue and cell samples are common clinical biological samples, mainly come from operations such as clinical operation, biopsy, puncture and the like, and can be used for pathological diagnosis and medical research by preparing slices, smears and extracting biological macromolecules. The quality of a sample, which is the basis of a medical study, determines to a large extent the accuracy and reliability of the study results. Long term storage of biological samples typically uses as low a temperature as possible to reduce biochemical reactions within the sample and to improve the stability of various components within the sample. Common storage temperatures for biomacromolecules, cells, tissues and organs are-800C (ultra-low temperature refrigerator), -1400C (liquid nitrogen gas phase or cryogenic refrigerator) and-1960C (liquid nitrogen liquid phase), the lower the temperature the longer the stable storage time of the sample.

The storage of the biological sample is not only simple to provide a stable low-temperature environment for the biological sample, but also relates to the access, management and the like of the biological sample, and belongs to the biological sample storage technology, and because the low-temperature environment for storing the biological sample is limited, an operator cannot work for a long time in the low-temperature environment, so that the intelligent storage of the biological sample is a direction worthy of development, and how to conveniently and quickly access the biological sample is realized.

Disclosure of Invention

Aiming at the problems, the invention provides an intelligent biological sample storage device based on water cooling and ultralow temperature.

The technical scheme of the invention is as follows: an intelligent biological sample storage device based on water-cooling ultra-low temperature mainly comprises a storage box, a water-cooling refrigerating device, an access window, a positioning access device and a touch control device,

the storage box is fixedly placed on the horizontal ground, the storage box comprises a storage part which is positioned at the rear half part of the storage box and used for storing biological samples and an access part which is positioned at the front half part of the storage box and used for accessing the biological samples, the storage part comprises a plurality of storage boxes layered at equal intervals, storage racks are movably arranged in the storage boxes, the storage racks are provided with a plurality of storage grooves in a matrix manner, both ends of the outer side of each storage rack are provided with hooks, a plurality of traction motors are arranged in the front side wall of the access part and correspond to the hooks, the traction motors are connected with the hooks through telescopic rods and used for pulling out or pushing the storage racks into the storage boxes,

the water-cooling refrigerating device comprises a refrigerating compressor and a condenser which are arranged on the front side of the storage box, the refrigerating compressor is communicated with the condenser and used for conveying the secondary refrigerant to the condenser in a pressurizing way, the condenser utilizes the refrigerant fluid circulating in the condenser to absorb heat and cool the secondary refrigerant passing through the condenser, the cooled secondary refrigerant is distributed in the storage box through a refrigerating coil pipe, the output end of the refrigerating coil pipe is connected with the input end of the refrigerating compressor and used for circulating the secondary refrigerant,

the access window is arranged above the refrigeration compressor and the condenser, the access window comprises an object placing groove which is arranged in the access window and used for placing a biological sample, a scanner which is arranged on the inner wall of the access window at one side of the object placing groove and used for scanning a biological sample label, a telescopic frame which is arranged at the bottom of the object placing groove and used for sending the object placing groove into the access part, and an electric sealing cover which is arranged at the joint of the access window and the access part, the electric sealing cover is opened when the biological sample is accessed, and cold air leakage in the access part is blocked after the access is finished,

the positioning access device is arranged above the access part and comprises two sliding tracks arranged above the left side and the right side of the access part and a sliding cross rod crossing the two sliding tracks and movably embedded on the sliding tracks, the sliding cross rod moves on the sliding cross rod through an electric roller I, a sliding block capable of moving on the sliding cross rod through an electric roller II is sleeved on the sliding cross rod, an electric lifting rod is arranged at the bottom end of the sliding block, a grabbing head for taking and placing biological samples is arranged at the bottom end of the electric lifting rod,

the touch control device is arranged on the outer wall of the storage box above the access window, and the touch control device is connected with the storage box, the water-cooling refrigerating device, the access window and the positioning access device through relays respectively.

Further, be equipped with the slide rail on the inside wall about the storage box respectively, the storage frame left and right sides corresponds the slide rail height department is equipped with the gyro wheel of sliding the scarf joint with the slide rail, and the rear end is all sealed before the slide rail, makes the storage frame pull out repeatedly and pushes in the storage box to avoid the storage frame to break away from the storage box.

Furthermore, baffle plates are arranged inside the condenser in a staggered mode, through holes are formed in the baffle plates, cold flow tubes penetrate through the through holes in the same horizontal line respectively, an inlet end for secondary refrigerant to enter is formed in the lower end of the condenser, and an outlet end for cooled secondary refrigerant to be output is formed in the upper end of the condenser, so that high-temperature secondary refrigerant can be fully contacted with the refrigerant, and cooling of the secondary refrigerant is accelerated.

Furthermore, the refrigeration coil is spirally arranged at the bottom side and the rear side of the storage box, the maximum area of the refrigeration coil is exposed in the storage box, the temperature in the storage box is absorbed and transferred out, and the low-temperature environment in the storage box is ensured.

Further, the access window outside is equipped with the movable cover of manual opening, the movable cover material is transparent glass, and the sliding cover that only reopens when the access biological sample can avoid outside dust impurity to lead to the fact the pollution to the biological sample through access window entering preservation incasement.

Further, the expansion bracket is including setting up the carriage in the inside bottom of access window, and the slip scarf joint is in carrier on the carriage, the carrier with put thing tank bottom end fixed connection, automatically deliver to the preservation incasement with biological sample, do not need operating personnel to contact low temperature environment, avoid low temperature environment to cause the injury to operating personnel.

Furthermore, the inside material that separates the temperature that all packs of lateral wall of saving the case guarantees the persistence of the interior low temperature environment of saving the case to even under the outage condition, the saving case also can keep low temperature environment under the effect that separates the temperature material.

The working principle of the invention is as follows: opening a movable cover of an access window, placing a biological sample test tube label to be stored in an object storage groove towards the left side and the right side, closing the movable cover, scanning the biological sample label by a scanner and transmitting the biological sample label to a touch control device for storage, distributing an empty storage groove for the biological sample, distributing the storage groove with a priority lower storage groove, after the distribution is finished, opening an electric sealing cover, moving the object storage groove to an access part of a storage box by a telescopic frame, moving a positioning access device on a sliding rail through a sliding cross rod, moving a sliding block on the sliding cross rod, moving a grabbing head above the biological sample, descending an electric lifting rod to enable the grabbing head to grab the biological sample to ascend, enabling the biological sample to be separated from the object storage groove, moving the object storage groove to an original position by the telescopic frame, closing the electric sealing cover, pulling a storage frame where the distribution storage groove is located by a traction motor, moving the biological sample into the distribution storage groove by the positioning access device and pushing the storage frame to the, and when the record is placed, the plane coordinate of a grabbing head of the positioning access device and the height coordinate of the working traction motor are transmitted to the touch control device for recording and storing, when the biological sample needs to be taken, only the label code of the biological sample needs to be input on the touch control device, the touch control device automatically matches the corresponding coordinate of the biological sample, the traction motor of the corresponding coordinate pulls out the storage rack, the positioning access device moves the grabbing head to the corresponding coordinate to grab the biological sample, the electric sealing cover is opened, the object placing groove is sent to the access part, the positioning access device places the biological sample in the object placing groove and moves into the access window, an operator opens the movable cover to take out the biological sample, and the storage groove for taking out the biological sample is marked as a blank state by the touch control device.

The invention has the beneficial effects that: the invention provides an intelligent biological sample storage device based on water cooling and ultralow temperature, which is particularly suitable for storing biological samples needing ultralow temperature storage, an operator only needs to place the biological samples on a storage groove in an access window through an arranged access window, a scanner can automatically scan biological sample label information and allocate blank storage grooves for the biological samples, the storage groove is conveyed into a storage box through an expansion bracket, the biological samples are grabbed through a positioning access device, meanwhile, a storage frame capable of being pulled out is arranged, the positioning access device places the biological samples on the storage groove of the storage frame, records coordinates and transmits the coordinates to a touch control device to correspond to the biological sample label code, when the biological samples are taken next time, the positioning access device can automatically take out the biological samples to be placed in the storage groove of the access window only by inputting the label code of the biological samples, the operator does not need to contact the low-temperature environment in the whole process, and the body cannot be damaged due to the low-temperature environment. In a word, the invention has the advantages of novel structure, convenient taking, safety, high efficiency and the like.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of the storage case structure of the present invention;

FIG. 3 is a schematic diagram of the condenser configuration of the present invention;

FIG. 4 is a schematic view of the refrigeration coil configuration of the present invention;

FIG. 5 is a schematic view of the telescoping mast construction of the present invention;

FIG. 6 is a schematic diagram of a positioning access device according to the present invention.

Wherein, 1-storage box, 11-storage part, 111-storage box, 1111-slide rail, 112-storage rack, 1121-roller, 113-storage tank, 114-hook, 12-access part, 121-traction motor, 122-telescopic rod, 2-water cooling refrigerating device, 21-refrigerating compressor, 22-condenser, 221-baffle plate, 222-through hole, 223-cold flow tube, 224-inlet end, 225-outlet end, 23-refrigerating coil pipe, 3-access window, 31-storage tank, 32-scanner, 33-telescopic rack, 331-sliding rack, 332-carrier rack, 34-electric sealing cover, 35-movable cover, 4-positioning access device, 41-slide rail, 42-slide rail, 421-electric roller I, 43-sliding block, 431-electric roller II, 44-electric lifting rod, 45-grabbing head and 5-touch control device.

Detailed Description

For the convenience of understanding the technical scheme of the present invention, the present invention is further explained with reference to the accompanying fig. 1 to 6 and the specific embodiments, which are not to be construed as limiting the scope of the present invention.

Example (b): as shown in figure 1, the intelligent biological sample storage device based on water cooling and ultralow temperature mainly comprises a storage box 1, a water cooling and refrigerating device 2, an access window 3, a positioning and accessing device 4 and a touch control device 5,

the storage box 1 is fixedly placed on a horizontal ground, the side walls of the storage box 1 are filled with aerogel felts made of thermal insulation materials, the storage box 1 comprises a storage part 11 positioned at the rear half part of the storage box 1 and used for storing biological samples and an access part 12 positioned at the front half part of the storage box 1 and used for accessing the biological samples, the storage part 11 comprises a plurality of storage boxes 111 layered at equal intervals, storage racks 112 are movably arranged in the storage boxes 111, as shown in figure 2, slide rails 1111 are respectively arranged on the left and right inner side walls of the storage boxes 111, rollers 1121 which are in sliding embedding connection with the slide rails 1111 are arranged at the positions corresponding to the heights of the slide rails 1111 at the left and right sides of the storage boxes 112, the front and rear ends of the slide rails 1111 are sealed, a plurality of storage grooves 113 are arranged on the storage racks 112 in a matrix form, hooks 114 are arranged at both ends of the outer side walls of each storage box 112, a plurality of traction motors 121 are arranged, for pulling out or pushing in the storage rack 112 into the storage box 111,

the water-cooled refrigerating device 2 comprises a refrigerating compressor 21 and a condenser 22 arranged at the front side of the storage box 1, the refrigerating compressor 21 is communicated with the condenser 22 and is used for conveying the secondary refrigerant into the condenser 22 under pressure, as shown in fig. 3, baffle plates 221 are arranged inside the condenser 22 in a staggered manner, through holes 222 are arranged on the baffle plates 221, cold flow pipes 223 respectively penetrate through the through holes 222 on the same horizontal line, an inlet end 224 for the secondary refrigerant to enter is arranged at the lower end of the condenser 22, an outlet end 225 for the cooled secondary refrigerant to be output is arranged at the upper end of the condenser 22, the condenser 22 absorbs heat for the secondary refrigerant passing through the condenser 22 by using the refrigerant fluid circulating inside the condenser, the cooled secondary refrigerant is distributed into the storage box 1 through a refrigerating coil 23, the output end of the refrigerating coil 23 is connected with the input end of the refrigerating compressor 21 and is used, the refrigerating coils 23 are spirally disposed at the bottom side and the rear side of the holding box 1,

the access window 3 is arranged above the refrigeration compressor 21 and the condenser 22, the access window 3 comprises an object placing groove 31 arranged inside the access window 3 for placing a biological sample, a scanner 32 arranged on the inner wall of the access window 3 at one side of the object placing groove 31 for scanning a biological sample label, an expansion bracket 33 arranged at the bottom of the object placing groove 31 for sending the object placing groove 31 into the access part 12, as shown in figure 5, the expansion bracket 33 comprises a sliding bracket 331 arranged at the bottom end inside the access window 1, an object carrier 332 glidingly embedded on the sliding bracket 331, the object carrier 332 is fixedly connected with the bottom end of the object placing groove 31, an electric sealing cover 34 arranged at the connection part of the access window 3 and the access part 12, the electric sealing cover 34 is opened when the biological sample is accessed, the cold air leakage in the access part 12 is blocked after the access is finished, a movable cover 35 manually opened is arranged outside the access window 3, and the movable cover 35 is made of transparent,

as shown in fig. 6, the positioning access device 4 is disposed above the access portion 12, the positioning access device 4 includes two sliding rails 41 disposed above the left and right sides of the access portion 12, a sliding cross bar 42 crossing over the two sliding rails 41 and movably embedded on the sliding rails 41, the sliding cross bar 42 moves on the sliding cross bar 42 through a first electric roller 421, a sliding block 43 capable of moving on the sliding cross bar 42 through a second electric roller 431 is sleeved on the sliding cross bar 42, an electric lifting rod 44 is disposed at the bottom end of the sliding block 43, a grabbing head 45 for taking and placing a biological sample is disposed at the bottom end of the electric lifting rod 44,

the touch control device 5 is arranged on the outer wall of the preservation box 1 above the access window 3, the touch control device 5 is respectively connected with the preservation box 1, the water-cooling refrigerating device 2, the access window 3 and the positioning access device 4 through a relay,

the traction motor 121 is a Wellmotion low-temperature-resistant servo motor, the telescopic rod 122 is a 24V low-temperature-resistant telescopic rod, the refrigeration compressor 21 is a G1000EL-160D3 horizontal scroll refrigeration compressor, the condenser 22 is a JF-50 tubular condenser, the scanner 32 is an NLS-NVF200 barcode scanner, the sliding frame 331 is an SB200L motor sliding seat, the first and second electric rollers are BLD-1009B electric rollers, the electric lifting rod 44 is a JIUYING direct-current electric lifting rod, the grabbing head 45 is a D2VW-5L2-1M small manipulator, and the touch control device 5 is an Amlogic intelligent touch control all-in-one machine.

The working method of the above embodiment is as follows: opening the movable cover 35 of the access window 3, placing the biological sample test tube label to be stored in the storage groove 31 towards the left side and the right side, closing the movable cover 35, scanning the biological sample label by the scanner 32 and transmitting the biological sample label to the touch control device 5 for storage, distributing the empty storage groove 113 for the biological sample by the touch control device 5, distributing the storage groove 113 with the priority of the lower storage groove 113, after the distribution is finished, opening the electric sealing cover 34, moving the storage groove 31 to the access part 12 of the storage box 1 by the telescopic frame 33, moving the positioning access device 4 on the sliding rail 41 by the sliding cross rod 42, moving the sliding block 43 on the sliding cross rod 42, moving the grabbing head 45 above the biological sample, descending the electric lifting rod 44 to make the grabbing head 45 grab the biological sample to rise, making the biological sample separate from the storage groove 31, moving the storage groove 31 to the original position by the telescopic frame 33, closing the electric sealing cover 34, meanwhile, the pulling motor 121 pulls out the storage rack 112 where the distribution storage groove 113 is located, the positioning access device 4 moves the biological sample into the distribution storage groove 113 and pushes the storage rack 112 to the original position, and the plane coordinate of the grabbing head 45 of the positioning access device 4 and the height coordinate of the working pulling motor 121 are transmitted to the touch control device 5 for recording and storing when recording and placing, when the biological sample needs to be taken, only the label code of the biological sample needs to be input on the touch control device 5, the touch control device 5 automatically matches the corresponding coordinate of the biological sample, the pulling motor 121 of the corresponding coordinate pulls out the storage rack 112, the positioning access device 4 moves the grabbing head 45 to the corresponding coordinate to grab the biological sample, the electric sealing cover 34 is opened, the storage groove 31 is sent into the access part 12, the positioning access device 4 places the biological sample in the storage groove 31 to move into the access window 3, the operator opens the movable cover 35 to take out the biological sample, and the touch control device 5 marks the storage tank 113 from which the biological sample is taken out as a blank state.

Claims

1. An intelligent biological sample storage device based on water-cooling ultra-low temperature is characterized by mainly comprising a storage box (1), a water-cooling refrigerating device (2), an access window (3), a positioning access device (4) and a touch control device (5),

the storage box (1) is fixedly placed on the horizontal ground, the storage box (1) comprises a storage part (11) which is positioned at the rear half part of the storage box (1) and is used for storing biological samples and an access part (12) which is positioned at the front half part of the storage box (1) and is used for accessing the biological samples, the storage part (11) comprises a plurality of storage boxes (111) which are layered at equal intervals, a storage rack (112) is movably arranged in each storage box (111), a plurality of storage grooves (113) are arranged on each storage rack (112) in a matrix manner, hooks (114) are arranged at two ends of the outer side of each storage rack (112), a plurality of traction motors (121) are arranged inside the front side wall of each access part (12) and correspond to the hooks (114), the traction motors (121) are connected with the hooks (114) through telescopic rods (122) and are used for pulling out or pushing the storage boxes (111) out of the storage racks (,

the water-cooling refrigerating device (2) is including setting up at refrigeration compressor (21) and condenser (22) of keeping case (1) front side, refrigeration compressor (21) with condenser (22) intercommunication for carry the secondary refrigerant pressurization to condenser (22) in, condenser (22) utilize the inside circulating refrigerant fluid of condenser to carry out the heat absorption cooling to the secondary refrigerant through condenser (22), the secondary refrigerant after the cooling distributes extremely through refrigeration coil (23) in keeping case (1), the output of refrigeration coil (23) with the input of refrigeration compressor (21) is connected for the circulation of secondary refrigerant,

the access window (3) is arranged above the refrigeration compressor (21) and the condenser (22), the access window (3) comprises an object placing groove (31) which is arranged inside the access window (3) and used for placing a biological sample, a scanner (32) which is arranged on the inner wall of the access window (3) at one side of the object placing groove (31) and used for scanning a biological sample label, an expansion bracket (33) which is arranged at the bottom of the object placing groove (31) and used for sending the object placing groove (31) into the access part (12), and an electric sealing cover (34) which is arranged at the joint of the access window (3) and the access part (12), wherein the electric sealing cover (34) is opened when the biological sample is accessed, and cold air in the access part (12) is blocked after the access is finished,

the positioning access device (4) is arranged above the access part (12), the positioning access device (4) comprises two sliding rails (41) arranged above the left side and the right side of the access part (12), a sliding cross rod (42) stretching across the two sliding rails (41) and movably embedded on the sliding rails (41), the sliding cross rod (42) moves on the sliding cross rod (42) through an electric roller I (421), a sliding block (43) capable of moving on the sliding cross rod (42) through an electric roller II (431) is sleeved on the sliding cross rod (42), an electric lifting rod (44) is arranged at the bottom end of the sliding block (43), and a grabbing head (45) for taking and placing biological samples is arranged at the bottom end of the electric lifting rod (44),

touch control device (5) set up on the case (1) outer wall of keeping in access window (3) top, touch control device (5) respectively through the relay with keep in case (1), water-cooling refrigerating plant (2), access window (3), location access arrangement (4) and connect.

2. The water-cooling ultra-low temperature-based intelligent biological sample storage device as claimed in claim 1, wherein slide rails (1111) are respectively arranged on the left and right inner side walls of the storage box (111), rollers (1121) which are in sliding engagement with the slide rails (1111) are arranged at the left and right sides of the storage rack (112) corresponding to the height of the slide rails (1111), and the front and rear ends of the slide rails (1111) are closed.

3. The intelligent water-cooled ultralow temperature-based biological sample storage device as recited in claim 1, wherein the refrigeration coil (23) is arranged at the bottom side and the rear side of the storage box (1) in a spiral shape.

4. The intelligent biological sample storage device based on water-cooling ultra-low temperature as claimed in claim 1, wherein a movable cover (35) which is manually opened is arranged outside the access window (3), and the movable cover (35) is made of transparent glass.

5. The water-cooled ultra-low temperature-based intelligent biological sample storage device as claimed in claim 4, wherein the movable cover (35) is made of transparent glass.

6. The intelligent biological sample storage device based on water-cooling ultra-low temperature as claimed in claim 1, wherein the telescopic rack (33) comprises a sliding rack (331) arranged at the bottom end inside the access window (1), a rack (332) slidably embedded on the sliding rack (331), the rack (332) is fixedly connected with the bottom end of the storage tank (31).

7. The water-cooling ultra-low temperature-based intelligent biological sample storage device as claimed in claim 1, wherein the side walls of the storage box (1) are filled with heat insulation materials.