CN115227807A - Gynecological gel containing recombinant human collagen and preparation method thereof - Google Patents
Gynecological gel containing recombinant human collagen and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a gynecological gel containing recombinant human collagen, which consists of the following raw materials in percentage by weight: 0.5-1.5% of recombinant human collagen, 3-7% of ceramide, 2-6% of nicotinamide, 0.6-1% of medical chitosan, 0.5-1.5% of ginseng extract, 0.2-0.6% of chamomile extract, 0.3-0.7% of purslane extract, 0.1-0.5% of aloe extract, 0.1-0.3% of natural lactic acid, 0.1-0.5% of polyhexamethylene biguanide, 0.3-0.7% of carbomer, 3-7% of propylene glycol, 1-5% of glycerol and the balance of purified water; the invention also provides a preparation method of the gynecological gel containing the recombinant human collagen. The gynecological gel has good antibacterial property and biocompatibility, is not easy to be diluted by vaginal secretion to reduce the effect, combines the recombinant human collagen with the traditional Chinese medicine components, has the functions of cleaning the wound surface, repairing the wound and promoting healing in the process of treating the female vaginal diseases, can supplement the collagen for the female pudendum, has a certain anti-aging effect, can maintain the stability of the vaginal micro-ecological environment, and has the double effects of repairing and treating.
Description
Technical Field
The invention relates to the technical field of gynecological multi-effect gel, in particular to gynecological multi-effect gel with an external antibacterial effect and an anti-aging effect of gynecological collagen supplementation.
Background
The investigation shows that the female gynecological diseases become one of the important diseases which disturb the health of the female, and mainly comprise: genital tract inflammation, peculiar smell, cervical erosion, cervical cancer and the like. And as the female ages, the loss of human collagen and the female fertility bring great disability to the health of the female reproductive system, so that the female reproductive system rapidly ages, the diseases and the reproductive system aging not only influence the fertility of the female, but also bring great harm to the physical and mental health of the female,
in the prior art, the treatment means of gynecological diseases mainly comprise: western medicine and antibiotic treatment, physical therapy and surgical treatment. Wherein, western medicine and antibiotic treatment are the most common means, but the method is easy to destroy the flora and acid-base balance of female genital tract, causing the conditions of low immunity, continuous inflammation and recurrent infection; physical therapy is a relatively healthy treatment means, including electric ironing, laser and the like, and can cause the locking of cervical secretory glands after long-term contact, so that cervical mucus is changed to cause infertility, and cervical dilatation disturbance during childbirth is easily caused; the operation treatment is the simplest treatment method and the treatment means with the greatest harm to the body, and the operation treatment is not recommended unless special conditions exist. The products related to the anti-aging and maintenance aspects of the female reproductive system in the market are more scarce.
Therefore, the problem to be solved by the technical personnel in the field is how to provide a multi-effect gel which has gynecological external antibacterial effect and gynecological external collagen supplementation anti-aging effect.
Disclosure of Invention
In view of the above, the invention provides a multi-effect gel with gynecological external antibacterial effect and gynecological external collagen-supplementing anti-aging effect and a preparation method thereof.
The product can solve the problem that western medicine antibiotics and hormones generate toxic and side effects on a human body, and meanwhile, the collagen is supplemented to the female pudendum to achieve the effects of resisting aging and maintaining.
In order to achieve the purpose, the invention adopts the following technical scheme:
the gynecological gel containing the recombinant human collagen is prepared from the following raw materials in percentage by weight:
0.5 to 1.5 percent of recombinant human collagen
3 to 7 percent of ceramide
2 to 6 percent of nicotinamide
Medical chitosan 0.6-1%
Ginseng extract 0.5-1.5%
0.2 to 0.6 percent of chamomile extract
0.3 to 0.7 percent of purslane extract
Aloe extract 0.1-0.5%
0.1 to 0.3 percent of natural lactic acid
0.1-0.5 thousandth of polyhexamethylene biguanide
Carbomer 0.3% -0.7%
3 to 7 percent of propylene glycol
1 to 5 percent of glycerin
The remainder was purified water.
Preferably, the material composition comprises the following raw materials in percentage by weight:
1 percent of recombinant human collagen
Ceramide 5%
4 percent of nicotinamide
Medical chitosan 0.8%
Ginseng radix extract 1%
Chamomile extract 0.4%
0.5 percent of purslane extract
Aloe extract 0.3%
0.2 percent of natural lactic acid
0.3 per mill of polyhexamethylene biguanide
Carbomer 0.5%
Propylene glycol 5%
Glycerol 3%
The remainder was purified water.
Preferably, the gynecological gel containing the recombinant human collagen is characterized in that:
the recombinant humanized collagen is based on the original gene sequence of human skin type III collagen, and the parts with strong water solubility and high biological activity are optimized and selected for codon optimization and splicing recombination to obtain a brand new recombinant humanized type III collagen sequence which has good water solubility and high biological activity, has better performance than human natural collagen, and has wide application prospect in the fields of biomedical materials, beauty cosmetics, food health care and the like.
The ceramide of the invention has the functions of maintaining skin barrier, moisturizing, resisting aging, whitening, treating diseases and the like.
The nicotinamide of the invention has the functions of repairing cuticle, resisting aging, whitening, locking water and keeping moisture on skin.
The medical chitosan of the invention is a gel matrix, wherein the molecular weight of the medical chitosan is 2000-5000, and the deacetylation degree is more than or equal to 95%, and the medical chitosan has the functions of antibiosis, restoration and moisture preservation.
The ginseng extract has the effects of moisturizing, promoting cell repair and regeneration, purifying skin and increasing elasticity.
The chamomile extract disclosed by the invention is flat in physical property and sweet in taste, and mainly has the effects of dispelling wind, relieving exterior syndrome, clearing heat and promoting diuresis. Has antibacterial, antiinflammatory, antihistaminic, antiallergic, spasmolytic, toxic materials clearing away, sunscreen, and skin caring effects.
The purslane extract has the functions of clearing heat and promoting diuresis, detoxifying and reducing swelling, diminishing inflammation, quenching thirst and promoting urination. Can be used for treating dysentery with red and white discharge, enteritis, gonorrhea: external use for treating furunculosis erysipelas
The aloe extract of the invention has the functions of purging fire; detoxification; removing blood stasis; and (4) killing insects. Red eyes are predominant; constipation; white turbidity; amenorrhea in women; hemorrhoids; scabies; carbuncle and furuncle; traumatic injury.
The natural lactic acid of the invention has the functions of moisture preservation, dissolution assistance, pH value regulation and corrosion prevention.
The polyhexamethylene biguanide has broad bactericidal spectrum; the effective concentration is low; the action speed is high; the property is stable; is easily soluble in water; can be used at normal temperature; long-term bacteriostasis without side effect; no corrosion; colorless and odorless; is nontoxic; the material is non-combustible, non-explosive and safe to use; it can be said to be the best bactericide. It is used for disinfecting skin and wound, and can be used for treating female pudendum with bacterial, mycotic, trichomonas, senile and nonspecific vaginitis, cervicitis, vulvitis, pudendum itch, etc., removing perineum odor, reducing secretion, cleaning and disinfecting pudendum before and after sexual intercourse, traveling on business trip, and before and after female menstrual period, preventing infection and transmission of dermatosis and STDs, and sterilizing and cleaning hands, feet, anus, etc.
The carbomer is a gel matrix and has the effects of moisturizing and forming.
The propylene glycol and the glycerol have the functions of moisturizing, lubricating, dissolving assisting and permeating.
A preparation method of gynecological gel containing recombinant human collagen is characterized by comprising the following preparation steps:
s1, dissolving the gel matrix medical chitosan and carbomer in purified water, and fully swelling for 30 hours to form a fluid A for later use;
s2, sequentially adding the glycerol and the propylene glycol into the solution A, and fully and uniformly stirring to obtain a mixed solution B for later use;
s3, fully dissolving the ceramide and the nicotinamide by using purified water at the temperature of 80 ℃, and uniformly stirring to obtain a mixed solution C for later use;
s4, mixing and uniformly stirring the recombinant human collagen, the ginseng extract, the chamomile extract, the purslane extract and the aloe extract to obtain a standby solution D;
s5, fully diluting the polyhexamethylene biguanide and the natural lactic acid with purified water to obtain a mixed solution E for later use;
s6, mixing the standby liquids B, C, D and E, stirring for 15 minutes at the speed of 250-350 revolutions per minute in the environment of 50 ℃,
filling into pressure-resistant bottles to prepare the gynecological gel finished product.
The invention has the beneficial effects that:
1. through the matching of various materials of recombinant human collagen, ceramide, nicotinamide, medical chitosan, ginseng extract, chamomile extract, purslane extract, aloe extract, carbomer, propylene glycol and glycerol, the skin-friendly property of the gel can be well improved, water and nutrient substances can be effectively supplemented into the skin, the quality of skin cells is favorably improved, and the purposes of moisturizing and whitening skin, deep purification, epidermis repairing, skin refreshing and anti-aging are achieved, wherein the traditional Chinese medicine extract can also achieve the effects of expelling wind and relieving exterior syndrome, clearing heat and promoting diuresis, resisting bacteria and diminishing inflammation, detoxifying and diminishing swelling, diminishing inflammation, leucorrhea with reddish discharge, purging fire, detoxifying, removing blood stasis, amenorrhea of women, scabies and carbuncle furuncle pyogenic infections; traumatic injury.
2. The polyhexamethylene biguanide has the advantages of low effective concentration, high action speed, stable property, long-term bacteriostasis, no side effect and no corrosiveness, has good effects on bacterial, mycotic, trichomonas, senile and nonspecific vaginitis, cervicitis, vulvitis, pudendum pruritus and other symptoms of female pudendum, removes perineal peculiar smell and reduces secretion.
In conclusion, the gynecological gel disclosed by the invention has an antibacterial repair function, has good biocompatibility, cell affinity and mechanical properties, has excellent water permeation, air permeation or drainage and moisture absorption effects, has the effects of resisting bacteria and diminishing inflammation, reducing wound infection and quickly relieving wound pain, has the effects of relieving pigmentation and promoting wound healing in a wound healing period, and has the effects of repairing epidermal wounds and tightening and resisting aging of aging skin on skin; can also be used for female vulva and vagina antibiosis, cleaning and anti-aging repair, can promote vagina collapse and damaged mucosa and tissue repair, recover vagina skin tissue elasticity, maintain vagina microenvironment stability, and promote vagina health.
Drawings
FIG. 1 is a flow chart of the preparation method of the gynecological gel containing the recombinant human collagen provided by the invention.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and examples, which are provided for the purpose of illustration only and are not intended to limit the scope of the invention.
Example 1
The gynecological gel containing the recombinant human collagen consists of the following raw materials in percentage by weight:
0.5 to 1.5 percent of recombinant human collagen
Ceramide 3% -7%
2 to 6 percent of nicotinamide
0.6 to 1 percent of medical chitosan
0.5 to 1.5 percent of ginseng extract
0.2 to 0.6 percent of chamomile extract
0.3 to 0.7 percent of purslane extract
0.1-0.5% of aloe extract
0.1 to 0.3 percent of natural lactic acid
0.1-0.5 thousandth of polyhexamethylene biguanide
Carbomer 0.3% -0.7%
3 to 7 percent of propylene glycol
1 to 5 percent of glycerin
The remainder was purified water.
In the invention, the recombinant humanized collagen is based on the original gene sequence of human skin type III collagen, and the parts with strong water solubility and high biological activity are optimized and selected for codon optimization and splicing recombination to obtain a brand new recombinant humanized type III collagen sequence which has good water solubility and high biological activity and has better performance than human natural collagen; ceramides (Ceramides ) exist in all eukaryotic cells, and have important regulation effects on cell differentiation, proliferation, apoptosis, aging and other vital activities. Ceramide is used as a main component of intercellular lipid of stratum corneum of skin, is not only used as a second messenger molecule in a sphingomyelin pathway, but also plays an important role in the formation process of the stratum corneum of the skin, and has the effects of maintaining skin barrier, preserving moisture, resisting aging, whitening, treating diseases and the like; nicotinamide can prevent pellagra. Has effects in protein and sugar metabolism, and can improve nutrition of human and animal; the medical chitosan is a gel matrix and has an inhibiting effect on the growth of various bacteria; the ginseng extract is warm in nature, sweet in taste, slightly bitter and slightly warm; the chamomile extract is bitter, pungent and slightly cold in physical property, has the effects of clearing heat and eliminating dampness, and has the functions of resisting bacteria, diminishing inflammation and relieving pain; the purslane extract has sour and cold physical properties and tastes, and has the effects of clearing heat and removing toxicity, cooling blood and stopping bleeding and stopping dysentery; aloe extract is bitter in taste, cold in nature, and has toxic materials clearing away effect; removing blood stasis; killing pests. Blood-shot eyes; constipation; white turbidity; amenorrhea in women.
In the invention, the polyhexamethylene biguanide is a colorless to light amber solution, and the polyhexamethylene biguanide is a derivative of a high molecular polymer and is used as a main disinfection and sterilization component; carbomer is white loose powder, has characteristic slight odor, is a high molecular polymer of acrylic acid bonded allyl sucrose or pentaerythritol allyl ether, and is a gel matrix; propylene glycol is colorless, slightly viscous and sweet liquid, and glycerin is colorless, odorless, sweet and clear and viscous liquid in appearance.
Example 2
The gynecological gel containing the recombinant human collagen is prepared from the following raw materials in percentage by weight:
the recombinant human collagen 1% is a high molecular biological protein produced by reverse transcribing mRNA of human collagen into cDNA by a biological engineering technology, enzyme-digested section of gene recombination and E.coli (Escherichia coli) in a high-density fermentation, separation, renaturation and purification process, the molecular weight of the collagen is 1500-3000 daltons and is more easily absorbed by a human body, wherein the collagen with the molecular weight of about 2000 daltons is most stable and easily absorbed by the human body, the recombinant human collagen 5 has the advantages of leading, high repairability, promotion of fibroblast growth, high safety, scientific research of gene engineering achievements, no virus hidden danger, no rejection reaction, high moisture retention, water permeability to dermis, high compatibility, gene sequence being 100% same as that of human collagen, high sensitivity resistance and promotion of cell growth to form a protective barrier.
In the invention, ceramide 5% is a phospholipid taking ceramide as a skeleton, which mainly comprises ceramide phosphorylcholine and ceramide phosphorylethanolamine, the phospholipid is a main component of a cell membrane, 40-50% of sebum in a horny layer is composed of ceramide, the ceramide is a main part of intercellular matrix and plays an important role in keeping the balance of horny layer moisture, the ceramide exists in all eukaryotic cells and has an important regulation effect on cell differentiation, proliferation, apoptosis, aging and other life activities, and the ceramide is a main component of intercellular lipid of the horny layer of the skin and plays an important role in the formation process of the horny layer of the epidermis as a second messenger molecule in a sphingomyelin pathway and has the effects of maintaining skin barrier, moisturizing, resisting aging, whitening, treating diseases and the like.
In the invention, 4% of nicotinamide is a derivative of vitamin B3, is also a skin anti-aging component recognized in the field of beauty skin science, and is more and more emphasized in recent years, the most important effect in the aspect of skin anti-aging is to relieve and prevent dull complexion, yellowing and vegetable color of the skin generated in the early aging process, certainly, the contribution of nicotinamide to the skin is far more than the above effects, the damaged stratum corneum lipid barrier can be repaired, the skin resistance is improved, and the effects which can be ignored by people are the deep water locking effect of the nicotinamide, so the skin care product containing the nicotinamide has extremely strong water locking and moisturizing effects.
In the invention, 0.8 percent of medical chitosan has inhibition effect on the growth of various bacteria and shows the characteristics similar to antibiotics, the chitosan has inhibition effect on experimental strains such as staphylococcus epidermidis, staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, candida albicans and the like, even on candida albicans which are difficult to inhibit by common antibiotics, the original structure of the cell wall of the bacteria is damaged by the interaction of positive charges carried by the chitosan and negative charges carried by the cell membrane of the microorganisms, the leakage of cell components is caused to play an antibacterial role, and the small molecular chitosan generates similar flocculation effect by biological macromolecules which permeate into the cells and carry anions, disturbs the normal physiological function of the cells, blocks the biosynthesis of DNA, thereby inhibiting the propagation and growth of the bacteria, the chitosan can promote the regeneration of epithelial cells, promote wound healing by mediating cell proliferation, attract a large amount of polymorphonuclear cells and macrophages by mild acute inflammatory reaction to clear tissue fragments and blood, promote the migration of the fibroblasts, have chemotaxis, migration and activation effects on the stromal cells, and accelerate cell proliferation and tissue remodeling processes, promoting the repair of skin tissues, and the degradation product N-acetylglucosamine (NAG) of chitosan is very important for repairing scars of tissues.
In the invention, 1% of ginseng extract is warm in nature, sweet in taste, slightly bitter and slightly warm in taste, and has effects of invigorating spleen and lung channel, and can be prepared into health food for resisting fatigue, resisting aging and nourishing brain in the medical health industry; in the cosmetic sanitary product industry, the product can be prepared into a sanitary product for removing freckles, reducing wrinkles, activating skin cells and enhancing skin elasticity.
In the invention, 0.4% of chamomile extract is bitter in taste, pungent and slightly cold in nature, has the effects of clearing heat and eliminating dampness, has the effects of resisting bacteria and diminishing inflammation, relieving pain, and can improve allergy-relieving effects such as young beans, allergy, eczema, burn and scald in the aspect of skin care; has good effects on improving irregular menstruation, menstrual pain and the like of women.
In the invention, the purslane extract accounts for 0.5 percent, and the nature taste is sour and cold; it enters liver and large intestine meridians; has effects in clearing away heat and toxic materials, cooling blood, stopping bleeding, and relieving dysentery; preventing skin from drying and aging, increasing skin comfort, scavenging free radicals, and improving skin comfort; can be used for resisting oxidation, resisting bacteria and inhibiting common pathogenic dermatophytes.
In the invention, the aloe extract is 0.3 percent, bitter in taste and cold in nature; the aloe containing 75 elements belongs to the liver and large intestine channels, almost completely coincides with substances needed by human cells, has obvious health care value, is called as 'magic plant' and 'family kit' by people, has the effects of promoting healing, immunological regeneration and the like for human bodies, has good nutrition, moistening and whitening effects on human skin by polysaccharide and multiple vitamins contained in the aloe, has the performances of astringing, softening, moisturizing, diminishing inflammation and bleaching the skin, has the effects of relieving sclerosis and keratinization and improving scars, can prevent small wrinkles, eye bags and skin laxity, and can keep the skin moist and tender.
In the invention, 0.2% of natural lactic acid can well achieve the effects of corrosion prevention, sterilization, pH value regulation and the like.
In the invention, polyhexamethylene biguanide 0.3 per mill is a derivative of a high molecular polymer, and is used as a main disinfection and sterilization component, guanidino in the component has high activity in polymerization reaction, so that the polymerized derivative is electropositive, and is easily adsorbed by various bacteria and viruses which are usually electronegative, thereby inhibiting the division function of the bacteria and the viruses, leading the bacteria and the viruses to lose the reproductive function, and a film formed by the polymer blocks the respiratory channel of the microorganisms, leading the microorganisms to suffocate and lose the vitality so as to achieve the sterilization effect; has the advantages of broad-spectrum sterilization, low effective concentration, high action speed, stable property, easy water dissolution, long-term bacteriostasis, no side effect, no corrosivity, no color, no odor, no toxicity, safe use and the like.
In the present invention, carbomer 0.5% is the gel matrix, while having: the carbomer has the effects of protecting the skin, reducing the stimulation and damage of substances with irritation to the skin and mucous membranes due to certain affinity with the skin, enhancing the resistance of the skin to ultraviolet rays and reducing the damage of the ultraviolet rays to the skin due to ultraviolet ray resistance, reducing the viscosity, keeping the stability of effective substances due to the fact that the carbomer has certain looseness and is a slightly acidic substance with high dilutability, and reducing the viscosity of the substances due to the fact that a proper amount of carbomer can be added when a gel or a sanitary product is manufactured, and diminishing inflammation and sterilizing effects, wherein the carbomer is a natural medicinal component and can sterilize, diminish inflammation and sterilize
In the present invention, propylene glycol 5%, a colorless, slightly viscous and sweet liquid, can be used as a humectant in conjunction with glycerin or sorbitol in hygiene products, toothpaste and soap.
In the invention, 3% of glycerin, also called glycerin, has a chemical formula of C3H8O3, is colorless, odorless, sweet in taste, clear and viscous in appearance, is an organic substance, can absorb moisture from air, and mainly plays roles of lubricating and moisturizing.
In the invention, the rest of the purified water has the resistivity of more than 0.1x106 omega cm when the temperature is more than 25 ℃, the purified water is water prepared for medicine by a distillation method, an ion exchange method, a reverse osmosis method or other suitable methods, and does not contain any additive, and the properties are as follows: is a colorless clear liquid; no odor and no taste.
TABLE 1
According to the test of GB15979-2002 standard, table 1 shows that the product does not irritate the skin or cause allergic reaction when the gel is subjected to a closed sensitization test method in the invention.
TABLE 2
Table 2 shows that in the comparative experiment of the gel on the promotion of the growth of the epithelial tissue, the growth rate of the epithelial tissue at each sampling point of the formula product of the invention is higher than that of a comparative group, and the promotion of the skin repair and the anti-aging effect are very good.
TABLE 3
According to GB15979-2002 standard detection table 3, the gel bacteriostasis comparative test proves that the bacteriostasis rate of each sampling point of the formula product is higher than that of a comparative group, and the gel antibacterial agent can effectively resist bacteria.
Referring to fig. 1, the invention also provides a preparation method of the gynecological gel containing the recombinant human collagen, which comprises the following steps:
s1, dissolving the gel matrix medical chitosan and carbomer in purified water, and fully swelling for 30 hours to form a fluid A for later use;
s2, sequentially adding the glycerol and the propylene glycol into the solution A, and fully and uniformly stirring to obtain a mixed solution B for later use;
s3, fully dissolving the ceramide and the nicotinamide by using purified water at the temperature of 80 ℃, and uniformly stirring to obtain a mixed solution C for later use;
s4, mixing and uniformly stirring the recombinant human collagen, the ginseng extract, the chamomile extract, the purslane extract and the aloe extract to obtain a standby solution D;
s5, fully diluting the polyhexamethylene biguanide and the natural lactic acid with purified water to obtain a mixed solution E for later use;
s6, mixing the standby liquids B, C, D and E, stirring for 15 minutes at the speed of 250-350 revolutions per minute in the environment of 50 ℃,
filling into pressure-resistant bottle, and making into gynecological gel.
In the invention, raw materials and mixing devices are prepared according to the mass ratio, the gel matrix medical chitosan and carbomer are dissolved in purified water and fully swelled for 30 hours to form a fluid A for later use; sequentially adding the glycerol and the propylene glycol into the A, and fully and uniformly stirring to obtain a mixed solution B for later use; fully dissolving the ceramide and the nicotinamide by using purified water with the temperature of 80 ℃, and uniformly stirring to obtain a mixed solution C for later use; mixing the recombinant human collagen, the ginseng extract, the chamomile extract, the purslane extract and the aloe extract, and uniformly stirring to obtain a standby solution D; fully diluting the polyhexamethylene biguanide and the natural lactic acid with purified water to obtain a mixed solution E for later use; mixing the standby liquid B, the standby liquid C, the standby liquid D and the standby liquid E, stirring for 15 minutes at the speed of 250-350 r/min in an environment of 50 ℃, filling into a pressure-resistant bottle, and preparing into gynecological gel.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (8)
1. The gynecological gel containing the recombinant human collagen is characterized by comprising the following raw materials in percentage by weight:
0.5 to 1.5 percent of recombinant human collagen
Ceramide 3% -7%
2 to 6 percent of nicotinamide
0.6 to 1 percent of medical chitosan
0.5 to 1.5 percent of ginseng extract
Chamomile extract 0.2-0.6%
0.3 to 0.7 percent of purslane extract
0.1-0.5% of aloe extract
0.1 to 0.3 percent of natural lactic acid
0.1-0.5 thousandth of polyhexamethylene biguanide
Carbomer 0.3% -0.7%
3 to 7 percent of propylene glycol
1 to 5 percent of glycerin
The remainder was purified water.
2. The gynecological gel containing the recombinant human collagen according to claim 1, which is characterized by comprising the following raw materials in percentage by weight:
1 percent of recombinant human collagen
Ceramide 5%
4 percent of nicotinamide
Medical chitosan 0.8%
Ginseng radix extract 1%
Chamomile extract 0.4%
0.5 percent of purslane extract
Aloe extract 0.3%
0.2 percent of natural lactic acid
Polyhexamethylene biguanide 0.3 ‰
Carbomer 0.5%
Propylene glycol 5%
3 percent of glycerin
The remainder was purified water.
3. The gynecological gel containing recombinant human collagen according to claim 1, characterized in that: the recombinant human collagen is a high molecular biological protein produced by reverse transcription of mRNA of human collagen into cDNA by a biological engineering technology, enzyme digestion, gene recombination and high-density fermentation, separation, renaturation and purification processes in escherichia coli; the ceramide is a phospholipid with ceramide as a skeleton, and mainly comprises ceramide phosphorylcholine and ceramide phosphorylethanolamine; said nicotinamide is a derivative of vitamin B3; the medical chitosan has an inhibiting effect on the growth of various bacteria; the ginseng extract is warm in nature, sweet in taste, slightly bitter and slightly warm; the chamomile extract is bitter, pungent and slightly cold in physical property, has the effects of clearing heat and eliminating dampness, and has the functions of resisting bacteria, diminishing inflammation and relieving pain; the herba Portulacae extract has sour and cold physical properties and taste, and has effects of clearing heat and detoxicating, cooling blood and stopping bleeding, and stopping dysentery; the aloe extract is bitter in taste and cold in nature; the purified water is water with resistivity of more than 0.1x106 omega cm when the temperature is more than 25 ℃.
4. The medical dressing containing the recombinant human collagen according to claim 1, wherein: said nicotinamide is white crystalline powder, odorless or almost odorless, bitter in taste, and slightly hygroscopic.
5. The medical dressing containing the recombinant human collagen according to claim 1, wherein: the polyhexamethylene biguanide is a colorless to light amber solution, and the polyhexamethylene biguanide is a derivative of a high molecular polymer and is used as a main disinfection and sterilization component.
6. The medical dressing containing the recombinant human collagen according to claim 1, wherein: the carbomer is white loose powder, has characteristic micro-odor and is a high molecular polymer of acrylic acid bonded allyl sucrose or pentaerythritol allyl ether.
7. The medical dressing containing the recombinant human collagen according to claim 1, wherein: the propylene glycol is colorless, slightly viscous and sweet liquid, and the glycerol is colorless, odorless, sweet and clear and viscous liquid in appearance.
8. A preparation method of gynecological gel containing recombinant human collagen is characterized by comprising the following preparation steps:
s1, dissolving the gel matrix medical chitosan and carbomer in purified water, and fully swelling for 30 hours to form a fluid A for later use;
s2, sequentially adding the glycerol and the propylene glycol into the solution A, and fully and uniformly stirring to obtain a mixed solution B for later use;
s3, fully dissolving the ceramide and the nicotinamide by using purified water at the temperature of 80 ℃, and uniformly stirring to obtain a mixed solution C for later use;
s4, mixing and uniformly stirring the recombinant human collagen, the ginseng extract, the chamomile extract, the purslane extract and the aloe extract to obtain a standby solution D;
s5, fully diluting the polyhexamethylene biguanide and the natural lactic acid with purified water to obtain a mixed solution E for later use;
s6, mixing the standby liquids B, C, D and E, stirring for 15 minutes at the speed of 250-350 revolutions per minute in the environment of 50 ℃,
filling into pressure-resistant bottles to prepare the gynecological gel finished product.
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