CN103766601A - Method for producing fermented feed protein by adopting mobile fermentation - Google Patents
Method for producing fermented feed protein by adopting mobile fermentation Download PDFInfo
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- CN103766601A CN103766601A CN201410075907.4A CN201410075907A CN103766601A CN 103766601 A CN103766601 A CN 103766601A CN 201410075907 A CN201410075907 A CN 201410075907A CN 103766601 A CN103766601 A CN 103766601A
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Abstract
The invention relates to a method for producing fermented feed protein by adopting mobile fermentation, and belongs to the technical field of feed processing. The method comprises the following concrete steps: by adopting plant feed protein or a mixture of plant feed protein and low-value aquatic product protein as a fermentation raw material, inoculating bacillus, yeasts and lactic acid bacteria, adding water, uniformly mixing, and controlling the water content of a uniformly mixed material to be 50%-70%; adding an oxygen producer, a water absorbent and water into a waterproof breathable film bag, sealing, and putting the sealed waterproof breathable film bag into a silica gel breathable film feed fermentation bag; putting the mixed fermentation raw material into the silica gel breathable film feed fermentation bag with the oxygen producer, and sealing for fermenting at 20-40 DEG C for 2-10 days. According to the method, the mixed fermentation raw material is bagged and transported to a farm and then is directly used, the fermentation of the mixed fermentation raw material in transportation and storage processes is automatically finished, the process is simple, the cost is low, and the fermented feed protein is high in nutritional value and good in raising effect.
Description
Technical field
The present invention relates to a kind of processing method of fermented feed protein, be specifically related to a kind of method that the fermentation raw material mixing is automatically completed in transportation and storage to the portable fermenting and producing fermented feed protein of fermentation, belong to feed processing technology field.
Background technology
In recent years, the fermented feed protein take fermented bean dregs as representative has obtained a large amount of application in aquaculture, but current China
The fermented feed protein overwhelming majority used is the fermented feed protein through super-dry processing.Dry fermented feed protein product is that the forage protein fermenting is prepared from through these aftertreatment technologys of super-dry, pulverizing and packing, and the cost of these aftertreatment technologys accounts for a greater part of of fermented bean dregs production cost.In addition, the forage protein fermenting whole digestive ferment inactivation in dry run, most of probio death, and the fragrance matter with luring function producing in sweat can vapor away.Therefore,, for plant, the use cost that uses the fresh not dry fermented feed protein of processing not only can save fermented feed protein, can also improve feeding effect.Therefore, be badly in need of a kind of portable fermentation technique of forage protein, complete the pack of fermentation raw material at fermentation plant, be then transported to plant's Direct-fed (automatically completing fermentation in transportation and storage).
Current domestic fermented feed protein manufacturer adopts " multiple bacteria compound fermentation technique ", and earlier fermentation utilizes bud
Spore bacillus aerobic fermentation produces the plurality of enzymes such as protease, cellulase, mannonase zytase by macromolecular substances degraded in raw material, and then the anaerobic fermentation that carries out saccharomycete and lactic acid bacteria is removed the nonprotein antigen in forage protein.
Number of patent application is that 201210495545. 5 patent " a kind of method that adopts portable fermentation and efficient raw material to produce highly effective biological feed " discloses a kind of method that adopts portable fermenting and producing fermented feed, after mixing by fermentation raw material and bacterial classification, install in silica gel respiratory membrane feed fermentation bag, and sealing and fermenting, in moving process, can complete fermentation.But the method mainly for be anaerobe fermentation, and fermented feed protein fermentation is in earlier stage to need sufficient aerobic fermentation, and quality its decisive role of aerobic fermentation to forage protein product after fermenting in early stage, thereby above portable fermentation process be not suitable for forage protein and carry out movable type and ferment.
Patent CN201010603355.1 discloses a kind of distribution packaging protein pulp fermented feed protein and processing method thereof, in fish slurry, chitin waste liquid, shrimp head slurry, add bacterial classification, molasses, pineapple peel juice, add the vegetable feed proteins such as dregs of beans to mix the protein milk of mixed bacteria again, leading portion aerobic fermentation completes in normal fermentation pond or windrow, after the compressed packing sealing of back segment anaerobic fermentation raw material, in storage and transport process, completes.But above forage protein leading portion aerobic fermentation completes in normal fermentation pond, and only the anaerobic fermentation in later stage completes in movement, and operating process is still loaded down with trivial details, and cost is relatively high.
The invention provides a kind of method that adopts portable fermenting and producing fermented feed protein, the fermentation raw material mixing is installed in the silica gel respiratory membrane feed fermentation bag of oxygenation agent, sealing and fermenting, then being transported to plant directly uses, this not only reduces the production cost of fermented feed protein greatly, retained whole probios and digestive ferment, feeding effect is better simultaneously.
Summary of the invention
The object of this invention is to provide a kind of method that adopts portable fermenting and producing fermented feed protein, by the fermentation raw material pack mixing, then being transported to plant directly uses, make it in transportation and storage, automatically complete fermentation, technique is simple, cost is low, and the forage protein fermenting is of high nutritive value, and feeding effect is good.
In order to address the above problem, the technical solution adopted in the present invention is:
A method that adopts portable fermenting and producing fermented feed protein, comprises the steps:
(1) take the mixture of vegetable feed proteins or vegetable feed proteins and low-value aquatic product protein as fermentation raw material, access gemma bar
Bacterium, saccharomycete and lactic acid bacteria, and add water and mix, the material moisture after mixing is controlled at 50%~70%;
(2) oxygenation agent, water absorbing agent and water are joined in waterproof ventilated membrane bag and sealed, the waterproof ventilated membrane bag of good seal joins
In silica gel respiratory membrane feed fermentation bag;
(3) fermentation raw material mixing is installed to above and be equipped with in the silica gel respiratory membrane feed fermentation bag of oxygenation agent, 20~40 ℃ close
Seal ferment 2~10 days.
Vegetable feed proteins described in step (1) is dregs of beans, peanut meal, the dish dregs of rice or Cottonseed Meal; Described low-value aquatic product protein
For leftovers of tilapia, shrimp head or aquatic products processing wastewater.
Bacillus described in step (1) is bacillus subtilis or bacillus licheniformis; Saccharomycete used is fodder yeast
Or saccharomyces cerevisiae; Lactic acid bacteria used is Lactobacillus delbrueckii, lactobacillus bulgaricus or streptococcus thermophilus.
The described inoculum concentration that adds bacterial classification of step (1) is: account for the percentage of fermentation raw material gross mass in the quality of the culture medium that contains this bacterial classification, be respectively: bacillus subtilis 0.1%~1%, saccharomycete 0.1%~0.5%, lactic acid bacteria 0.1%~2%.
Oxygenation agent described in step (2) is the one in calper calcium peroxide and SODIUM PERCARBONATE, and oxygenation agent is block, and the addition of oxygenation agent is that every 20 kilograms of fermentation raw materials add oxygenation agent 50~200g.
Water described in step (2) is blocks of solid ice, and the addition of water is that every 20 kilograms of fermentation raw materials add water 50~600g.
Water absorbing agent described in step (2) is the one in anhydrous calcium chloride, anhydrous sodium sulfate, and water absorbing agent is Powdered, and the addition of water absorbing agent is that every 20 kilograms of fermentation raw materials add water absorbing agent 50~200g.
Waterproof ventilated membrane described in step (2) is eptfe membrane.
the invention has the beneficial effects as follows:
(1) fermenting and producing of existing fermented feed protein all completes at fermentation plant, and owing to considering the fresh-keeping problem of product, the product needed finally making is dry, thereby the fermented feed protein that fermentation plant provides is at present all dry fermented feed protein.After fermentation, forage protein is through drying program, not only increase the production cost of fermented feed protein, in addition, the forage protein fermenting whole digestive ferment inactivation in dry run, most of probio death, and the fragrance matter with luring function producing in sweat can vapor away.Portable fermentation process provided by the invention, fermentation is controlled simpler, after fermentation raw material pack, without manual control, can automatically complete fermentation.And, owing to automatically having completed fermentation in moving process, forage protein after fermentation is without dry, plant can not add dry fermented feed protein by Direct-fed, this not only reduces the production cost of fermented feed protein greatly, retained whole probios and digestive ferment, feeding effect is better simultaneously.
(2) portable fermentation process provided by the invention is more easy, whole sweat all can complete in movement, can promote not to be dried the fermented feed protein of processing in the extensive application of plant, this not only can reduce the production cost of fermented feed protein, and can also improve feeding effect, a kind of nonreactive breeding way is also provided simultaneously, has had a good application prospect.
The specific embodiment
Below by embodiment, the present invention is described in further details, these embodiment are only used for illustrating the present invention, do not limit the scope of the invention.
embodiment 1
After dregs of beans is pulverized, cross 60 mesh sieves for subsequent use, toward the bacillus subtilis that adds 0.1% on dregs of beans, 0.5% fodder yeast, 2% Lactobacillus delbrueckii, then mix adding water, and the water content of the material after mixing is controlled at 50%; Block 50g calper calcium peroxide, 600g blocks of solid ice and the Powdered anhydrous calcium chloride of 200g are installed in eptfe membrane bag and sealing, and then the eptfe membrane bag that oxygenation agent is housed is above put in silica gel respiratory membrane feed fermentation bag, finally again toward packing 20 kilograms of fermentation raw materials sealing and fermenting bag in silica gel respiratory membrane feed fermentation bag above; 20 ℃ of sealing and fermenting 10 days.
embodiment 2
After peanut meal is pulverized, cross 60 mesh sieves for subsequent use, toward the bacillus licheniformis that adds 1% on peanut meal, 0.1% saccharomyces cerevisiae, 0.1% streptococcus thermophilus, then mix adding water, and the water content of the material after mixing is controlled at 70%; Block 200g SODIUM PERCARBONATE, 50g blocks of solid ice and the Powdered anhydrous sodium sulfate of 50g are installed in eptfe membrane bag and sealing, and then the eptfe membrane bag that oxygenation agent is housed is above put in silica gel respiratory membrane feed fermentation bag, finally again toward packing 20 kilograms of fermentation raw materials sealing and fermenting bag in silica gel respiratory membrane feed fermentation bag above; 40 ℃ of sealing and fermenting 2 days.
embodiment 3
After the dish dregs of rice are pulverized, cross 60 mesh sieves for subsequent use, toward the bacillus subtilis that adds 0.8% on the dish dregs of rice, 0.5% fodder yeast, 0.3% lactobacillus bulgaricus, then mix adding water, and the water content of the material after mixing is controlled at 60%; Block 100g SODIUM PERCARBONATE, 500g blocks of solid ice and the Powdered anhydrous calcium chloride of 80g are installed in eptfe membrane bag and sealing, and then the eptfe membrane bag that oxygenation agent is housed is above put in silica gel respiratory membrane feed fermentation bag, finally again toward packing 20 kilograms of fermentation raw materials sealing and fermenting bag in silica gel respiratory membrane feed fermentation bag above; 25 ℃ of sealing and fermenting 4 days.
embodiment 4
After rubbing, leftovers of tilapia and dregs of beans mix (the two ratio is 1:1), toward the bacillus subtilis that adds 0.4% on raw material above, 0.2% fodder yeast, 0.7% lactobacillus bulgaricus, then mix adding water, and the water content of the material after mixing is controlled at 55%; Block 80g SODIUM PERCARBONATE, 500g blocks of solid ice and the Powdered anhydrous calcium chloride of 70g are installed in eptfe membrane bag and sealing, and then the eptfe membrane bag that oxygenation agent is housed is above put in silica gel respiratory membrane feed fermentation bag, finally again toward packing 20 kilograms of fermentation raw materials sealing and fermenting bag in silica gel respiratory membrane feed fermentation bag above; 35 ℃ of sealing and fermenting 7 days.
embodiment 5
After rubbing, leftovers of tilapia and peanut meal mix (the two ratio is 1:1), toward the bacillus subtilis that adds 0.5% on raw material above, 0.2% fodder yeast, 1% streptococcus thermophilus, then mix adding water, and the water content of the material after mixing is controlled at 62%; Block 50g SODIUM PERCARBONATE, 300g blocks of solid ice and the Powdered anhydrous calcium chloride of 80g are installed in eptfe membrane bag and sealing, and then the eptfe membrane bag that oxygenation agent is housed is above put in silica gel respiratory membrane feed fermentation bag, finally again toward packing 20 kilograms of fermentation raw materials sealing and fermenting bag in silica gel respiratory membrane feed fermentation bag above; 25 ℃ of sealing and fermenting 5 days.
Claims (8)
1. a method that adopts portable fermenting and producing fermented feed protein, is characterized in that: comprise the steps:
(1) take the mixture of vegetable feed proteins or vegetable feed proteins and low-value aquatic product protein as fermentation raw material, access gemma bar
Bacterium, saccharomycete and lactic acid bacteria, and add water and mix, the material moisture after mixing is controlled at 50%~70%;
(2) oxygenation agent, water absorbing agent and water are joined in waterproof ventilated membrane bag and sealed, the waterproof ventilated membrane bag of good seal joins
In silica gel respiratory membrane feed fermentation bag;
(3) fermentation raw material mixing is installed to above and be equipped with in the silica gel respiratory membrane feed fermentation bag of oxygenation agent, 20~40 ℃ close
Seal ferment 2~10 days.
2. according to a kind of method that adopts portable fermenting and producing fermented feed protein claimed in claim 1, it is characterized in that: the vegetable feed proteins described in step (1) is dregs of beans, peanut meal, the dish dregs of rice or Cottonseed Meal; Described low-value aquatic product protein is leftovers of tilapia, shrimp head or aquatic products processing wastewater.
3. according to a kind of method that adopts portable fermenting and producing fermented feed protein claimed in claim 1, it is characterized in that: the bacillus described in step (1) is bacillus subtilis or bacillus licheniformis; Saccharomycete used is fodder yeast or saccharomyces cerevisiae; Lactic acid bacteria used is Lactobacillus delbrueckii, lactobacillus bulgaricus or streptococcus thermophilus.
4. a kind of method that adopts portable fermenting and producing fermented feed protein according to claim 1, it is characterized in that: the described inoculum concentration that adds bacterial classification of step (1) is: the percentage that accounts for fermentation raw material gross mass in the quality of the culture medium that contains this bacterial classification, be respectively: bacillus subtilis 0.1%~1%, saccharomycete 0.1%~0.5%, lactic acid bacteria 0.1%~2%.
5. according to a kind of method that adopts portable fermenting and producing fermented feed protein claimed in claim 1, it is characterized in that: the oxygenation agent described in step (2) is the one in calper calcium peroxide and SODIUM PERCARBONATE, oxygenation agent is block, and the addition of oxygenation agent is that every 20 kilograms of fermentation raw materials add oxygenation agent 50~200g.
6. according to a kind of method that adopts portable fermenting and producing fermented feed protein claimed in claim 1, it is characterized in that: the water described in step (2) is blocks of solid ice, the addition of water is that every 20 kilograms of fermentation raw materials add water 50~600g.
7. according to a kind of method that adopts portable fermenting and producing fermented feed protein claimed in claim 1, it is characterized in that: the water absorbing agent described in step (2) is the one in anhydrous calcium chloride, anhydrous sodium sulfate, water absorbing agent is Powdered, and the addition of water absorbing agent is that every 20 kilograms of fermentation raw materials add water absorbing agent 50~200g.
8. according to a kind of method that adopts portable fermenting and producing fermented feed protein claimed in claim 1, it is characterized in that: the waterproof ventilated membrane described in step (2) is eptfe membrane.
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104186924A (en) * | 2014-08-01 | 2014-12-10 | 福建龙岩金和动物饲料有限公司 | Automatic production process of environment-friendly nonreactive fermented feed |
CN106615702A (en) * | 2016-09-19 | 2017-05-10 | 湛江五洲生物工程有限公司 | Wet process deep fermentation method of fermented bean pulp |
CN106922948A (en) * | 2015-12-29 | 2017-07-07 | 丰益(上海)生物技术研发中心有限公司 | The production technology of fermented bean dregs is carried out suitable for edible fat production factory environment |
CN108770993A (en) * | 2018-05-18 | 2018-11-09 | 广西开田三农科技有限责任公司 | A kind of preparation method of polypeptide feed |
CN111772058A (en) * | 2020-08-04 | 2020-10-16 | 清远海贝生物技术有限公司 | Active compound feed suitable for aquaculture and preparation method and application thereof |
CN116158489A (en) * | 2023-04-19 | 2023-05-26 | 内蒙古海邻科技发展有限公司 | Method for producing biological fermentation feed by using saccharomyces cerevisiae |
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JPS6112281A (en) * | 1984-06-27 | 1986-01-20 | Kikkoman Corp | Cultivation of aerobic microorganism |
CN102550804A (en) * | 2012-02-04 | 2012-07-11 | 广东海洋大学 | Method for preparing feed protein by mixed fermentation of tilapia leftovers and soybean meals |
CN102987058A (en) * | 2012-11-28 | 2013-03-27 | 张有聪 | Method for producing efficient biological feed by using movable type fermentation and high-energy raw materials |
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2014
- 2014-03-04 CN CN201410075907.4A patent/CN103766601A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6112281A (en) * | 1984-06-27 | 1986-01-20 | Kikkoman Corp | Cultivation of aerobic microorganism |
CN102550804A (en) * | 2012-02-04 | 2012-07-11 | 广东海洋大学 | Method for preparing feed protein by mixed fermentation of tilapia leftovers and soybean meals |
CN102987058A (en) * | 2012-11-28 | 2013-03-27 | 张有聪 | Method for producing efficient biological feed by using movable type fermentation and high-energy raw materials |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104186924A (en) * | 2014-08-01 | 2014-12-10 | 福建龙岩金和动物饲料有限公司 | Automatic production process of environment-friendly nonreactive fermented feed |
CN106922948A (en) * | 2015-12-29 | 2017-07-07 | 丰益(上海)生物技术研发中心有限公司 | The production technology of fermented bean dregs is carried out suitable for edible fat production factory environment |
CN106615702A (en) * | 2016-09-19 | 2017-05-10 | 湛江五洲生物工程有限公司 | Wet process deep fermentation method of fermented bean pulp |
CN108770993A (en) * | 2018-05-18 | 2018-11-09 | 广西开田三农科技有限责任公司 | A kind of preparation method of polypeptide feed |
CN111772058A (en) * | 2020-08-04 | 2020-10-16 | 清远海贝生物技术有限公司 | Active compound feed suitable for aquaculture and preparation method and application thereof |
CN116158489A (en) * | 2023-04-19 | 2023-05-26 | 内蒙古海邻科技发展有限公司 | Method for producing biological fermentation feed by using saccharomyces cerevisiae |
CN116158489B (en) * | 2023-04-19 | 2023-07-18 | 内蒙古海邻科技发展有限公司 | Method for producing biological fermentation feed by using saccharomyces cerevisiae |
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Application publication date: 20140507 |