CN103027188A - Deep processing method of pineapple skin and residues - Google Patents
Deep processing method of pineapple skin and residues Download PDFInfo
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- CN103027188A CN103027188A CN2013100158063A CN201310015806A CN103027188A CN 103027188 A CN103027188 A CN 103027188A CN 2013100158063 A CN2013100158063 A CN 2013100158063A CN 201310015806 A CN201310015806 A CN 201310015806A CN 103027188 A CN103027188 A CN 103027188A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a deep processing method of pineapple skin and residues. After fruit juice or preserved fruits are produced from pineapples, fruit skin and fruit residues which are produced are taken as wastes generally used as auxiliary feed for cultivation of domestic animals without any treatment, and the extensive low-grade treatment way can cause the huge waste of resources and simultaneously produce greater pressure on an environment. According to the deep processing method, rhizopus, candida, lactobacillus, an active enzyme and EM (effective microorganism) original dew are utilized, and the specific technical steps of primary fermentation of the pineapple skin and residues, secondary fermentation, squeezing, fermentation treatment of juice, preparation of a probiotic preparation and the like are further performed to prepare the fermented feed and the probiotic preparation. The detection shows that the content of crude protein in the fermented feed achieves 23%, and the content of the crude protein after washing achieves 16%. The probiotic preparation can purify water quality, has the effects of promoting the growth of animals, enhancing immunity and disease resistance and the like, becomes a product for replacing antibiotics in cultivation industry and has good using effects in cultivation of cattle, sheep and aquatic products.
Description
Technical field
What the present invention relates to is the deep working method of a kind of pineapple peel, slag, belongs to fruit deep process technology field.
Background technology
Pineapple is the peculiar fruit of subtropical zone, and the maturity period concentrates, the maturity period weak point of holding time, and shelf life is short, when fruit is ripe, must digest at short notice, otherwise perishable rotting.Therefore, except few part pineapple directly eats, most of pineapples are to produce fruit juice or preserved fruit as raw material, after producing fruit juice or preserved fruit, the pericarp pomace that produces in the process normally gives the livestock culturing family as auxiliary fodder as discarded object, without any processing, all is spontaneous fermentation basically, rot easily, utilization rate is very low.This extensive rudimentary processing pineapple peel, slag mode not only cause the huge waste of resource, also cause larger pressure to environment simultaneously.Pineapple peel, slag have characteristics that are different from very much other pomace, are exactly that sugar is abundant, and hemicellulose level is very high, therefore retentiveness is good, and water content reaches 40%～50%, is the good source of carbon source, can be the microbial fermentation utilization, be a kind of good fermentation matrix.Aspect the utilizing of pineapple peel, slag, publication No. is CN102150746A, title is the patent application of " a kind of pineapple bran packaged fermented feed and energy-saving processing method ", take pineapple bran as main material, through broken juice, add dregs of rice class and leavening, fast just drying, die mould bagging, pack, the operation such as solid state fermentation, modulate and be processed into the extraordinary fermented feed of the livestocks such as ruminant, deer, pig such as milk cow.This patent application has utilized pineapple bran to carry out deep processing, but sweat is the spontaneous fermentation process, and resulting product is still more elementary, to the utilization of pineapple peel, slag, in fact also has great potentialities and can dig.The progress of biotechnology is endless, utilizes biotechnology to pineapple peel, slag recycling, turn waste into wealth, and for fruit juice preserved fruit manufacturing enterprise, be a kind of active measures that improve added value of product, reduce product cost, protection of the environment.
Summary of the invention
The deep working method that the purpose of this invention is to provide a kind of pineapple peel, slag, utilize pineapple peel, slag to be raw material, by adopting related microorganisms fermentation, enzyme preparation technology and probiotics micro-ecological formulation technology that pineapple peel, apple pomace resource are carried out deep development, produce simultaneously protein feed and probiotics preparation.Overcome at present low to the existing added value of product of utilizing of pineapple peel, slag, the deficiency that cost is high.
For achieving the above object, the present invention has taked following technical scheme, and the deep working method of a kind of pineapple peel, slag comprises following concrete steps:
(1) pineapple peel, slag primary fermentation: get rhizopus, candidiasis and Bacillus acidi lactici by 3.4～4.2: 6.3～7.5: 0.7～1.2 volume ratio, three kinds of bacterium liquid are mixed to enlarge be cultured in the bacterium liquid bacterium number greater than 8,000 ten thousand/gram, be inoculated in pineapple peel after preparation fruit juice, the preserved fruit, the slag and ferment, total inoculum concentration is 6～9% of pineapple peel, slag weight, adjust pH value 3.2～4.0,30 ℃～34 ℃ of fermentation temperatures, material water weight ratio 1: 1, thickness of feed layer 6.5cm～7.5cm, fermented 20-30 hour, and obtained the primary fermentation pericarp, slag is for subsequent use;
(2) secondary fermentation: in primary fermentation pericarp, slag, add and actively send the beautiful R2 enzyme 150-200ppm of continuous heavy rain and active HAZYME DCL enzyme 50-80ppm greater than 8000U greater than 15000U, continue fermentation 5～6 days, fermentation condition is pH value 3.2～4.0,30 ℃～34 ℃ of fermentation temperatures obtain the secondary fermentation pericarp, slag is for subsequent use;
(3) squeezing: get the secondary fermentation pericarp, slag is squeezed the juice by filter press, the fermented feed and the fermentation juice that obtain water content 30-35% after squeezing the juice are for subsequent use;
(4) fermentation juice is processed: fermentation juice left standstill 6-8 hour, get supernatant and carry out twice filtration, carrying out for the first time 50 order low-pressures filters, carry out for the second time 200 order centrifugal filtrations, remove pomace and foreign material, fermentation juice after the filtering is heated to 75～80 ℃, kept 5-8 minute, then being cooled to 25-35 ℃, to obtain filtering juice for subsequent use;
(5) preparation probiotics preparation: above-mentioned filtering juice is put into agitator stir, place culture tank, make in the tank fluid temperature keep 30～35 ℃, the former dew of EM, the urea of weight percentage 0.45～0.55% and 0.15～0.2% the potassium dihydrogen phosphate that add liquid volume percentage composition 12～14%, activate bacterial classification, carry out bacterial classification and enlarge cultivation, the activity index of the liquid probiotic bacterium in culture tank surpasses 3 * 10
8During individual viable bacteria/g, reach the probiotics preparation product requirement, minute install in the packing container and get final product.
The former dew of described rhizopus, candidiasis, Bacillus acidi lactici and EM all has the commercial goods;
Resulting product of the present invention is not higher than in temperature under 30 ℃ the dry environment to be preserved.
Take the present invention of above-mentioned measure, in preparation process, obtained two kinds of goods, first fermented feed, it two is probiotics preparations.After testing, wherein, the fermented feed crude protein content has reached 23%, and the crude protein content after washing has reached 16%, and 12 seed amino acid content of detection all have a very significant increase.The goods fragrant odour, palatability is greatly improved, and is the mycoprotein energy feed of beef cattle, milk cow, mutton sheep.Another goods probiotics preparation of gained of the present invention can purify water, animal had growth promotion, the effects such as the immunity of enhancing animal and resistance against diseases, can not cause that the cultivated animals drug disposition is residual, be to replace antibiotic product in aquaculture, result of use is good in cattle and sheep and aquaculture.
Use-case:
(1) drinking-water:
Probiotics preparation adds mixing in 1000～2000 times the drinking-water to, freely drink every day for livestock and poultry, can prevent and treat general intestines problem, various mass-sending diseases and infectious disease, common disease are had control, preventive effect, reach the use that reduces medicine, save the medication spending and can reach 80%.
(2) silage making:
The ensilage of chopping is filled in the silage silo by layer, and every cubic metre of ensilage adds 0.2 kilogram of probiotics preparation.Whenever dose one deck ensilage (about 20～30 centimetres) and spray a probiotics preparation, until fill up, sealed fermenting after the compacting, plastic covering cloth.This fermented feed cooperated with conventional feed be used for cultivation, can Cost reduction 12～25%, the more conventional feeding day weightening finish of sucking pig increases about 10%, and growing and fattening pigs generally can shift to an earlier date listing in 5～7 days; Broiler chicken (duck) can shift to an earlier date listing in 2～3 days.
The specific embodiment
Embodiment 1
(1) pineapple peel, slag primary fermentation: get rhizopus, candidiasis and Bacillus acidi lactici by 3.4～: 6.3: 0.7～volume ratio, three kinds of bacterium liquid are mixed to enlarge be cultured in the bacterium liquid bacterium number greater than 8,000 ten thousand/gram, be inoculated in pineapple peel after preparation fruit juice, the preserved fruit, the slag, total inoculum concentration is 6% of pineapple peel, slag weight, adjust pH value 3.2,30 ℃ ℃ of fermentation temperatures, material water weight ratio 1: 1, thickness of feed layer 6.5cm～7.5cm, fermented 20 hours, and obtained the primary fermentation pericarp, slag is for subsequent use;
(2) secondary fermentation: in primary fermentation pericarp, slag, add and actively send the beautiful R2 enzyme 150ppm of continuous heavy rain and active HAZYME DCL enzyme 50ppm greater than 8000U greater than 15000U, continue fermentation 5～6 days, fermentation condition is pH value 3.2,30 ℃ ℃ of fermentation temperatures obtain the secondary fermentation pericarp, slag is for subsequent use;
(3) squeezing: get the secondary fermentation pericarp, slag is squeezed the juice by filter press, the fermented feed and the fermentation juice that obtain water content 30% after squeezing the juice are for subsequent use;
(4) fermentation juice is processed: fermentation juice left standstill 6 hours, get supernatant and carry out twice filtration, carrying out for the first time 50 order low-pressures filters, carry out for the second time 200 order centrifugal filtrations, remove pomace and foreign material, fermentation juice after the filtering is heated to 75 ℃, kept 8 minutes, then being cooled to 25 ℃, to obtain filtering juice for subsequent use;
(5) preparation probiotics preparation: above-mentioned filtration clear juice is put into agitator stir, place culture tank, make in the tank fluid temperature keep 30 ℃, the former dew of EM of adding liquid volume percentage composition 12%, 0.45% urea and 0.15% potassium dihydrogen phosphate, activate bacterial classification, carry out bacterial classification and enlarge cultivation, the activity index of the liquid probiotic bacterium in culture tank surpasses 3 * 10
8During individual viable bacteria/g, reach the probiotics preparation product requirement, minute install in the packing container and get final product.
Embodiment 2
(1) pineapple peel, slag primary fermentation: get rhizopus, candidiasis and Bacillus acidi lactici by 4.2: 7.5: 1.2 volume ratio, three kinds of bacterium liquid are mixed to enlarge be cultured in the bacterium liquid bacterium number greater than 8,000 ten thousand/gram, be inoculated in pineapple peel after preparation fruit juice, the preserved fruit, the slag, total inoculum concentration is 9% of pineapple peel, slag weight, adjust pH value 4.0,34 ℃ of fermentation temperatures, material water weight ratio 1: 1, thickness of feed layer 6.5cm～7.5cm, fermented 30 hours, and obtained the primary fermentation pericarp, slag is for subsequent use;
(2) secondary fermentation: in primary fermentation pericarp, slag, add and actively send the beautiful R2 enzyme 200ppm of continuous heavy rain and active HAZYME DCL enzyme 80ppm greater than 8000U greater than 15000U, continue fermentation 5～6 days, fermentation condition is pH value 4.0,34 ℃ of fermentation temperatures obtain the secondary fermentation pericarp, slag is for subsequent use;
(3) squeezing: get the secondary fermentation pericarp, slag is squeezed the juice by filter press, the fermented feed and the fermentation juice that obtain water content 35% after squeezing the juice are for subsequent use;
(4) fermentation juice is processed: fermentation juice left standstill 6 hours, get supernatant and carry out twice filtration, carrying out for the first time 50 order low-pressures filters, carry out for the second time 200 order centrifugal filtrations, remove pomace and foreign material, fermentation juice after the filtering is heated to 80 ℃, kept 5 minutes, then being cooled to 25-35 ℃, to obtain filtering juice for subsequent use;
(5) preparation probiotics preparation: above-mentioned filtration clear juice is put into agitator stir, place culture tank, make in the tank fluid temperature keep 35 ℃, the former dew of EM of adding liquid volume percentage composition 14%, 0.55% urea and 0.2% potassium dihydrogen phosphate, activate bacterial classification, carry out bacterial classification and enlarge cultivation, the activity index of the liquid probiotic bacterium in culture tank surpasses 3 * 10
8During individual viable bacteria/g, reach the probiotics preparation product requirement, minute install in the packing container and get final product.
Embodiment 3
(1) pineapple peel, slag primary fermentation: get rhizopus, candidiasis and Bacillus acidi lactici by 4.0: 7.0: 1.0 volume ratio, three kinds of bacterium liquid are mixed to enlarge be cultured in the bacterium liquid bacterium number greater than 8,000 ten thousand/gram, be inoculated in pineapple peel after preparation fruit juice, the preserved fruit, the slag, total inoculum concentration is 8% of pineapple peel, slag weight, adjust pH value 3.6,30 ℃ of fermentation temperatures, material water weight ratio 1: 1, thickness of feed layer 6.5cm～7.5cm, fermented 24 hours, and obtained the primary fermentation pericarp, slag is for subsequent use;
(2) secondary fermentation: in primary fermentation pericarp, slag, add and actively send the beautiful R2 enzyme 180ppm of continuous heavy rain and active HAZYME DCL enzyme 60ppm greater than 8000U greater than 15000U, continue fermentation 6 days, fermentation condition is pH value 3.6, and 32 ℃ of fermentation temperatures obtain the secondary fermentation pericarp, slag is for subsequent use;
(3) squeezing: get the secondary fermentation pericarp, slag is squeezed the juice by filter press, the fermented feed and the fermentation juice that obtain water content 30% after squeezing the juice are for subsequent use;
(4) fermentation juice is processed: fermentation juice left standstill 6 hours, get supernatant and carry out twice filtration, carrying out for the first time 50 order low-pressures filters, carry out for the second time 200 order centrifugal filtrations, remove pomace and foreign material, fermentation juice after the filtering is heated to 80 ℃, kept 6 minutes, then being cooled to 30 ℃, to obtain filtering juice for subsequent use;
(5) preparation probiotics preparation: above-mentioned filtration clear juice is put into agitator stir, place culture tank, make in the tank fluid temperature keep 30～35 ℃, the former dew of EM of adding liquid volume percentage composition 12～14%, 0.45～0.55% urea and 0.15～0.2% potassium dihydrogen phosphate, activate bacterial classification, carry out bacterial classification and enlarge cultivation, the activity index of the liquid probiotic bacterium in culture tank surpasses 3 * 10
8During individual viable bacteria/g, reach the probiotics preparation product requirement, minute install in the packing container and get final product.
Claims (1)
1. the deep working method of a pineapple peel, slag is characterized in that described method comprises following concrete steps:
(1) pineapple peel, slag primary fermentation: get rhizopus, candidiasis and Bacillus acidi lactici by 3.4～4.2: 6.3～7.5: 0.7～1.2 volume ratio, three kinds of bacterium liquid are mixed to enlarge be cultured in the bacterium liquid bacterium number greater than 8,000 ten thousand/gram, be inoculated in pineapple peel after preparation fruit juice, the preserved fruit, the slag and ferment, total inoculum concentration is 6～9% of pineapple peel, slag weight, adjust pH value 3.2～4.0,30 ℃～34 ℃ of fermentation temperatures, material water weight ratio 1: 1, thickness of feed layer 6.5cm～7.5cm, fermented 20-30 hour, and obtained the primary fermentation pericarp, slag is for subsequent use;
(2) secondary fermentation: in primary fermentation pericarp, slag, add and actively send the beautiful R2 enzyme 150-200ppm of continuous heavy rain and active HAZYME DCL enzyme 50-80ppm greater than 8000U greater than 15000U, continue fermentation 5～6 days, fermentation condition is pH value 3.2～4.0,30 ℃～34 ℃ of fermentation temperatures obtain the secondary fermentation pericarp, slag is for subsequent use;
(3) squeezing: get the secondary fermentation pericarp, slag is squeezed the juice by filter press, the fermented feed and the fermentation juice that obtain water content 30-35% after squeezing the juice are for subsequent use;
(4) fermentation juice is processed: fermentation juice left standstill 6-8 hour, get supernatant and carry out twice filtration, carrying out for the first time 50 order low-pressures filters, carry out for the second time 200 order centrifugal filtrations, remove pomace and foreign material, fermentation juice after the filtering is heated to 75～80 ℃, kept 5-8 minute, then being cooled to 25-35 ℃, to obtain filtering juice for subsequent use;
(5) preparation probiotics preparation: above-mentioned filtering juice is put into agitator stir, place culture tank, make in the tank fluid temperature keep 30～35 ℃, the former dew of EM, the urea of weight percentage 0.45～0.55% and 0.15～0.2% the potassium dihydrogen phosphate that add liquid volume percentage composition 12～14%, activate bacterial classification, carry out bacterial classification and enlarge cultivation, the activity index of the liquid probiotic bacterium in culture tank surpasses 3 * 10
8During individual viable bacteria/g, reach the probiotics preparation product requirement, minute install in the packing container and get final product.
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Cited By (8)
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| CN104872418A (en) * | 2015-05-14 | 2015-09-02 | 广西大学 | Fruit and vegetable waste dreg fermented biological feed and preparation method thereof |
| JP5819018B1 (en) * | 2015-04-30 | 2015-11-18 | 千秋 櫛田 | Pineapple leaf stem crushed fertilizer formation method |
| CN107205434A (en) * | 2015-04-30 | 2017-09-26 | 栉田千秋 | The preparation method that broken pineapple leaves caulome is used as feed or animal foodstuff raw material, and broken pineapple leaves caulome are used as feed or animal foodstuff raw material |
| CN107593587A (en) * | 2017-09-21 | 2018-01-19 | 重庆农笑农业发展有限公司 | The high-efficiency breeding method of goat |
| CN109619294A (en) * | 2018-12-26 | 2019-04-16 | 湖北工业大学 | A kind of cattle feed and preparation method thereof made using Stauntonia latifolia |
| CN109805175A (en) * | 2018-12-29 | 2019-05-28 | 华南协同创新研究院 | A kind of acidulant, its compound bacteria one-step fermentation preparation method and application |
| CN110100952A (en) * | 2019-05-22 | 2019-08-09 | 王萌 | A method of feed liquid additive is prepared using pomace |
| CN110150464A (en) * | 2019-05-22 | 2019-08-23 | 张宪坤 | A kind of process system preparing feed liquid additive using pomace |
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Cited By (9)
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| CN104872418A (en) * | 2015-05-14 | 2015-09-02 | 广西大学 | Fruit and vegetable waste dreg fermented biological feed and preparation method thereof |
| CN107593587A (en) * | 2017-09-21 | 2018-01-19 | 重庆农笑农业发展有限公司 | The high-efficiency breeding method of goat |
| CN109619294A (en) * | 2018-12-26 | 2019-04-16 | 湖北工业大学 | A kind of cattle feed and preparation method thereof made using Stauntonia latifolia |
| CN109805175A (en) * | 2018-12-29 | 2019-05-28 | 华南协同创新研究院 | A kind of acidulant, its compound bacteria one-step fermentation preparation method and application |
| CN110100952A (en) * | 2019-05-22 | 2019-08-09 | 王萌 | A method of feed liquid additive is prepared using pomace |
| CN110150464A (en) * | 2019-05-22 | 2019-08-23 | 张宪坤 | A kind of process system preparing feed liquid additive using pomace |
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Application publication date: 20130410 |