CN103719006B

CN103719006B - A kind of method for improving knife long-tailed anchovy parent survival rate during artificial breeding

Info

Publication number: CN103719006B
Application number: CN201410026730.9A
Authority: CN
Inventors: 施永海; 张海明; 张根玉; 谢永德; 陆根海; 刘建忠; 徐嘉波; 刘永士
Original assignee: SHANGHAI AQUATIC PRODUCT TECHNOLOGY PROMOTION STATION; Shanghai Fisheries Research Institute
Current assignee: Shanghai Fisheries Research Institute Shanghai Fisheries Technical Extension Station
Priority date: 2014-01-21
Filing date: 2014-01-21
Publication date: 2017-06-06
Anticipated expiration: 2034-01-21
Also published as: CN103719006A

Abstract

A kind of method for improving knife long-tailed anchovy parent survival rate during artificial breeding, it is characterized in that in standby parent's breeding process, drawing in the net sediment upturning 23 times as the standby parent of artificial propagation using the 2 ages above filial generation knife long-tailed anchovy adult fish of artificial breeding, before artificial breeding, standby parent draws in the net to take exercise 23 times again；The complete knife long-tailed anchovy adult fish of gonad development is selected as parent；The parent of select is transported with shading dark drum, and operation will softly not from water；Oxytocic hormone dosage is no more than 30ug/Kg, and saline volume is no more than 0.5ml, natural fertilization per tail；Spawning inducing pool is round or square, shading regulation and control, continuous micro- inflation；After parent's spawning, postpartum reinforced cultivating is carried out, progressively add seawater to adjust salt to salinity 5 10,20 40 tails of breeding density/pond, continuous charge, 22 25 DEG C of water temperature feeds oppossum shrimp and Exopalaemon carinicauda daily 2 times, secondary feeding volume is fixed depending on food ration, bottom is inhaled 1 time daily, the fishes and shrimps of death are removed in time.

Description

A kind of method for improving knife long-tailed anchovy parent survival rate during artificial breeding

Technical field

The present invention relates to aquaculture, the method for parent survival rate during especially improving knife long-tailed anchovy artificial breeding.

Background technology

Knife long-tailed anchovy (Coilia nasusSchlegel), also known as jaw long-tailed anchovy long, hairtail, rough cutter fish are commonly called as, are that river sea is migratory Fish, are distributed mainly on China Huang Bohai Sea and the band of the East Sea one, and all maritime rivers are distributed, with THE LOWER YANGTZE VALLEY yield highest. Coilia ectenes in Yangtze River is strong and famous with fine and tender taste, fresh fertilizer, seasonal, and with the Changjiang river hilsa herring, filefish and claim " the Changjiang river three delicacies ", history Upper Coilia ectenes in Yangtze River resource is extremely enriched.In recent years, due to the influence of the factors such as overfishing and the deterioration of the ecological environment, knife Long-tailed anchovy resource drastically fails, and yield is in downward trend year by year and individual miniaturization is serious, and knife long-tailed anchovy can not form advantage in the Changjiang river Population, while the price of Coilia ectenes in Yangtze River marketable fish is also constantly rising.The breakthrough and popularization of the artificial breeding technology of knife long-tailed anchovy, and then push away Enter the industrialization breeding production of the fish, the adjustment of fishery constitution can be promoted, promote fishery synergy, fisherman's increased income.Therefore, Knife long-tailed anchovy has good development prospect as new varieties are propagated artificially.At present, the reproduction technique about knife long-tailed anchovy is studied also in first In the level stage, in recent years, domestic many scientific research institutions set up the project carry out the research of the correlation technique one after another, occasionally have knife long-tailed anchovy pond to be multiplied into Indivedual reports of work(, although Shanghai Aquatic Product Institute Fengxian base took the lead in obtaining in the knife long-tailed anchovy parent using culture in 2011 Obtained the success of indoor intensive artificial breeding, but had a fiery temperament due to knife long-tailed anchovy, artificial propagation misoperation and postpartum it is not good The reasons such as good cultivation, knife long-tailed anchovy parent survival rate is extremely low after artificial breeding, while also resulting in the ovum fertilization obtained by artificial propagation Rate is low, the ropy problem of newly hatched larvae, therefore it is knife long-tailed anchovy artificial propagation to improve knife long-tailed anchovy parent survival rate during artificial breeding Key technology.

The content of the invention

It is an object of the invention to provide a kind of method for improving knife long-tailed anchovy parent survival rate during artificial breeding, to adapt to knife Long-tailed anchovy scale is intensive to breed production.

Technical scheme is prepared by parent, parent selects, hasten parturition fertilization control, reinforced cultivating production stage group Into, it is characterized in that using artificial breeding 2 ages above filial generation knife long-tailed anchovy adult fish as artificial propagation standby parent, relative to The adult fish that wild or fish wild seed is propagated artificially, these filial generation knife long-tailed anchovy adult fish disposition passives, in standby parent In breeding process, sediment upturning 2-3 times is drawn in the net, while before artificial breeding, standby parent draws in the net to take exercise 2-3 times again, to reduce parent The stress reaction caused by artificial operation；Select gonad development completely, that is, select the knife long-tailed anchovy adult fish of the phase of gonad development 4 as parent This, otherwise develops the meeting mortality during hastening parturition without complete adult fish, selects parent's standard：Raun postabdomen expand and Softness, cloacal aperture is micro- red；Milter, light pressure cloacal aperture has a small amount of seminal fluid to flow out；The parent of select is using shading dark drum fortune It is defeated；Whole selecting will softly not from water with operation in transportation；Oxytocic hormone dosage is no more than 30ug/Kg, saline volume 0.5ml is no more than per tail, using heavy dose of hormone and Large Copacity physiological saline parent can't be caused dead；Using receiving naturally Smart mode, otherwise using artificial insemination, cause parent to receive during whether raun lays eggs and manually crowded ovum squeezes essence checking Wound and scale come off；Spawning inducing pool uses round or square pond, shading regulation and control, continuous micro- inflation, the same knife of daily management condition Long-tailed anchovy traditional breeding methods；After parent's spawning, postpartum reinforced cultivating, the postpartum same spawning inducing pool of culture pond condition are carried out, while progressively adding Seawater adjusts salt to salinity 5-10, and to play antiinflammation, breeding density is 20-40 tails/pond, and continuous charge, water temperature keeps 22-25 DEG C, feed oppossum shrimp and Exopalaemon carinicauda daily 2 times, depending on the feeding volume that food ration is fixed time, inhale bottom 1 time daily, remove in time dead Fishes and shrimps, postpartum cultivate the time be 7-10 days, by whole reproductive process, parent survival rate is in 85-95%.

The present invention is relative to the outstanding feature of prior art：Survival rate of the knife long-tailed anchovy during breeding is drastically increased, Survival rate is significantly improved in 85-95% relative to original 20-40%, meanwhile, the matter of embryonated egg and prelarva is also improve indirectly Amount, in addition, the method is easy to operate, method is practical, be adapted to the production of knife long-tailed anchovy propagation in scale, and is easy to be accepted and pushes away Extensively.

Specific embodiment

1st, parent prepares：2 ages above filial generation knife long-tailed anchovy adult fish using artificial breeding is close as the standby of artificial propagation This, in standby parent's breeding process, draws in the net sediment upturning 2-3 time, while before artificial breeding, standby parent draws in the net exercise 2-3 again It is secondary.

2nd, parent selects：Select gonad development completely, that is, select the knife long-tailed anchovy adult fish of the phase of gonad development 4 as parent, select Parent's standard：Raun postabdomen expands and soft, and cloacal aperture is micro- red；Milter, light pressure cloacal aperture has a small amount of seminal fluid to flow out；Select Parent using shading dark drum transport；Whole selecting will softly not from water with operation in transportation.

3rd, hasten parturition and control of being fertilized：Oxytocic hormone dosage is no more than 30ug/Kg, and saline volume is no more than 0.5ml per tail； Using natural fertilization mode, otherwise using artificial insemination, during checking whether raun lays eggs and manually crowded ovum squeezes essence Parent's injury and scale is caused to come off；Spawning inducing pool uses round or square pond, and round or square pool area of hastening parturition is 20 square metres, shading regulation and control, continuous micro- inflation, daily management condition is with knife long-tailed anchovy traditional breeding methods；

4th, postpartum reinforced cultivating：After parent's spawning, postpartum reinforced cultivating is carried out, the postpartum same spawning inducing pool of culture pond condition, together When progressively add seawater to adjust salt to salinity 5-10, breeding density is 20-40 tails/pond, and continuous charge, water temperature is kept for 22-25 DEG C, daily Feed oppossum shrimp and Exopalaemon carinicauda 2 times, secondary feeding volume fixed depending on food ration, inhale bottom 1 time daily, the fishes and shrimps of death are removed in time, Postpartum cultivated the time for 7-10 days, and by whole reproductive process, parent survival rate is in 85-95%.

Above-mentioned specific embodiment is in 5-July in 2013 point, 6 batches of warps in this institute Technique of Raising Seeedling centre base experiment knife long-tailed anchovy Parent, carries out whole artificial propagation, and 6 batches amount to and hasten parturition the tails of parent 279, by it is whole breed with postpartum cultivating process, parent is last Survive altogether 248 tails, average survival is 88.9%.

Claims

1. it is a kind of improve knife long-tailed anchovy parent survival rate during artificial breeding method, by parent prepare, parent select, fertilization of hastening parturition Control, reinforced cultivating composition, it is characterized in that using the 2 ages above filial generation knife long-tailed anchovy adult fish of artificial breeding as artificial propagation Standby parent, in standby parent's breeding process, draws in the net sediment upturning 2-3 times, and before artificial breeding, standby parent draws in the net to temper 2- again 3 times；Select gonad development completely, i.e., the knife long-tailed anchovy adult fish of the phase of gonad development 4 selects parent's standard as parent：Raun postabdomen Expand and soft, cloacal aperture is micro- red；Milter, light pressure cloacal aperture has a small amount of seminal fluid to flow out；The parent of select is dark using shading Drum is transported；Whole selecting will softly not from water with operation in transportation；Oxytocic hormone dosage is no more than 30ug/Kg, physiology Salt water is no more than 0.5ml per tail, using natural fertilization mode；Spawning inducing pool uses 20 square metres of round or square pond, Shading regulates and controls, continuous micro- inflation, and daily management is with knife long-tailed anchovy traditional breeding methods；After parent's spawning, postpartum reinforced cultivating is carried out, produced The same spawning inducing pool of culture pond condition afterwards, while progressively plus seawater adjusts salt to salinity 5-10, breeding density is 20-40 tails/pond, is continuously filled Gas, water temperature is kept for 22-25 DEG C, feeds oppossum shrimp and Exopalaemon carinicauda daily 2 times, depending on the feeding volume that food ration is fixed time, bottom 1 is inhaled daily It is secondary, dead fishes and shrimps are removed in time, postpartum cultivated the time for 7-10 days.

2. it is according to claim 1 it is a kind of improve knife long-tailed anchovy parent survival rate during artificial breeding method, it is characterized in that Round or square pool area of hastening parturition is for 20 square metres.