CN102524319B - Multiple-effect nanoscale biopesticide water aqua and use thereof - Google Patents
Multiple-effect nanoscale biopesticide water aqua and use thereof Download PDFInfo
- Publication number
- CN102524319B CN102524319B CN201110315898.8A CN201110315898A CN102524319B CN 102524319 B CN102524319 B CN 102524319B CN 201110315898 A CN201110315898 A CN 201110315898A CN 102524319 B CN102524319 B CN 102524319B
- Authority
- CN
- China
- Prior art keywords
- oil
- effect
- essential oil
- biopesticide
- water aqua
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 15
- 230000000853 biopesticidal effect Effects 0.000 title abstract description 12
- 241000196324 Embryophyta Species 0.000 claims abstract description 44
- 239000000341 volatile oil Substances 0.000 claims abstract description 36
- 239000000052 vinegar Substances 0.000 claims abstract description 28
- 235000021419 vinegar Nutrition 0.000 claims abstract description 28
- 239000000575 pesticide Substances 0.000 claims abstract description 20
- 239000003921 oil Substances 0.000 claims abstract description 10
- 235000019198 oils Nutrition 0.000 claims abstract description 10
- NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 claims abstract description 9
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims abstract description 8
- 229920000053 polysorbate 80 Polymers 0.000 claims abstract description 8
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims abstract description 7
- 235000017491 Bambusa tulda Nutrition 0.000 claims abstract description 7
- 244000082204 Phyllostachys viridis Species 0.000 claims abstract description 7
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims abstract description 7
- 239000011425 bamboo Substances 0.000 claims abstract description 7
- 244000045947 parasite Species 0.000 claims abstract description 6
- 241000723346 Cinnamomum camphora Species 0.000 claims abstract description 5
- 240000001238 Gaultheria procumbens Species 0.000 claims abstract description 5
- 235000007297 Gaultheria procumbens Nutrition 0.000 claims abstract description 5
- 239000010642 eucalyptus oil Substances 0.000 claims abstract description 5
- 229940044949 eucalyptus oil Drugs 0.000 claims abstract description 5
- 239000001525 mentha piperita l. herb oil Substances 0.000 claims abstract description 5
- OSWPMRLSEDHDFF-UHFFFAOYSA-N methyl salicylate Chemical compound COC(=O)C1=CC=CC=C1O OSWPMRLSEDHDFF-UHFFFAOYSA-N 0.000 claims abstract description 5
- 235000019477 peppermint oil Nutrition 0.000 claims abstract description 5
- 238000002360 preparation method Methods 0.000 claims abstract description 5
- 244000025254 Cannabis sativa Species 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 21
- 238000003756 stirring Methods 0.000 claims description 20
- 239000000839 emulsion Substances 0.000 claims description 14
- 239000012752 auxiliary agent Substances 0.000 claims description 7
- 239000002131 composite material Substances 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 239000010495 camellia oil Substances 0.000 claims description 4
- 239000010632 citronella oil Substances 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000013019 agitation Methods 0.000 claims description 2
- 230000000840 anti-viral effect Effects 0.000 claims description 2
- 239000000077 insect repellent Substances 0.000 claims description 2
- 238000009329 organic farming Methods 0.000 claims description 2
- 230000000361 pesticidal effect Effects 0.000 claims description 2
- 230000002195 synergetic effect Effects 0.000 claims description 2
- 239000002671 adjuvant Substances 0.000 abstract description 8
- 239000000203 mixture Substances 0.000 abstract description 8
- 241000256113 Culicidae Species 0.000 abstract description 3
- 241000238631 Hexapoda Species 0.000 abstract description 3
- 239000004480 active ingredient Substances 0.000 abstract description 2
- 239000010630 cinnamon oil Substances 0.000 abstract description 2
- 239000001941 cymbopogon citratus dc and cymbopogon flexuosus oil Substances 0.000 abstract description 2
- 230000001846 repelling effect Effects 0.000 abstract description 2
- WBIQQQGBSDOWNP-UHFFFAOYSA-N 2-dodecylbenzenesulfonic acid Chemical compound CCCCCCCCCCCCC1=CC=CC=C1S(O)(=O)=O WBIQQQGBSDOWNP-UHFFFAOYSA-N 0.000 abstract 1
- 241000700605 Viruses Species 0.000 abstract 1
- 239000010634 clove oil Substances 0.000 abstract 1
- 229940060296 dodecylbenzenesulfonic acid Drugs 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 239000010677 tea tree oil Substances 0.000 abstract 1
- 229940111630 tea tree oil Drugs 0.000 abstract 1
- 239000002023 wood Substances 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 19
- 241000255925 Diptera Species 0.000 description 15
- 230000000694 effects Effects 0.000 description 15
- 239000003905 agrochemical Substances 0.000 description 10
- 201000010099 disease Diseases 0.000 description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 9
- 239000003814 drug Substances 0.000 description 6
- 239000002917 insecticide Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 230000001139 anti-pruritic effect Effects 0.000 description 5
- 241000607479 Yersinia pestis Species 0.000 description 4
- 239000003995 emulsifying agent Substances 0.000 description 4
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 3
- 241000256057 Culex quinquefasciatus Species 0.000 description 3
- 241000257159 Musca domestica Species 0.000 description 3
- 238000012271 agricultural production Methods 0.000 description 3
- 239000003908 antipruritic agent Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 231100000518 lethal Toxicity 0.000 description 3
- 230000001665 lethal effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 241001124076 Aphididae Species 0.000 description 2
- 206010003399 Arthropod bite Diseases 0.000 description 2
- 241000254127 Bemisia tabaci Species 0.000 description 2
- 235000006008 Brassica napus var napus Nutrition 0.000 description 2
- 241001674939 Caulanthus Species 0.000 description 2
- 241000256054 Culex <genus> Species 0.000 description 2
- 241000500437 Plutella xylostella Species 0.000 description 2
- 230000002155 anti-virotic effect Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 210000002414 leg Anatomy 0.000 description 2
- 231100000225 lethality Toxicity 0.000 description 2
- 230000003449 preventive effect Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 1
- 240000003553 Leptospermum scoparium Species 0.000 description 1
- 235000015459 Lycium barbarum Nutrition 0.000 description 1
- 241000255972 Pieris <butterfly> Species 0.000 description 1
- 241000255969 Pieris brassicae Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000008821 health effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000013348 organic food Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 230000008786 sensory perception of smell Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000006273 synthetic pesticide Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a multiple-effect nanoscale biopesticide water aqua, a preparation method of the multiple-effect nanoscale biopesticide water aqua and use of the multiple-effect nanoscale biopesticide water aqua. The invention is characterized in that the multiple-effect nanoscale biopesticide water aqua comprises 5-40% of pesticides active ingredient-containing plant essential oil, 20-90% of plant vinegar with efficiency enhancing effect and 5-40% of pesticide adjuvant, wherein the plant vinegar is one of or a mixture of more than one of bamboo vinegar, wood vinegar and/or grass vinegar, the plant essential oil is one of or a mixture of more than one of wintergreen, camphor tree oil, clove oil, peppermint oil, eucalyptus oil, citionella oil, tea tree oil, lemongrass oil or cinnamon oil, and the pesticide adjuvant is one of or a mixture of more than one of dodecylbenzenesulfonic acid, tween-80, SPAN-80 or OP-10. The water aqua is characterized in that the water aqua is used for repelling mosquitos as well as the application of killing insects, expelling parasite and virus resistance in family agriculture, family flower, organic agriculture and green agriculture.
Description
Technical field
The invention belongs to technical field of pesticide preparations, be specially a kind of multiple-effect biological aqueous pesticide solution and application thereof.
Background technology
Agricultural chemicals is important capital goods in the agricultural sector, to control pest, tackles explosive pest disaster, ensures that agricultural production security has a very important role, and agricultural chemicals directly affects national food safety, agricultural product quality and safety and people's health simultaneously.Therefore, should be using agricultural chemicals as specialty goods, efficient, safety, economy, environment-protection pesticide should become the direction of Agrochemicals.Biopesticide be utilize that microorganism itself or its metabolite are anti-ly cured the disease, the preparation of worm, weeds etc., comprise farm antibiotics, bacterial pesticide, fungal farm chemicals and viral pesticide, have selectivity high, be easy to degraded, be difficult for accumulation, consumption is few, it is little to pollute, little to person poultry toxicity, environment compatibility good, damage by disease and insect is difficult for producing the advantages such as resistance, thereby more meets the requirement of modern society to agricultural production and agricultural chemicals.Development biopesticide, reduces the use of chemical synthetic pesticide, has become the new trend of global agricultural chemicals industry development.Now, people are more and more higher to the security requirement of agricultural chemicals, along with social development, in family and office, grow flower and plant the leisure health activity that agricultural has become more and more people, but traditional agricultural chemicals is owing to having the insecurity of knowing altogether, and expelling parasite desinsection and the antibacterial agricultural chemicals of sterilization that family is used are also seldom seen.
Summary of the invention
The problem existing for existing biopesticide, the present invention's design provides a kind of multiple-effect biological aqueous pesticide solution.This medicament has the multiple efficacies such as regulating crop growth, desinsection expelling parasite, sterilizing and anti-virus.
A kind of multiple-effect nanoscale biopesticide water aqua, it is characterized in that containing pesticide activity component plants essential oil 5-40%, the plant vinegar liquid 20-90% with synergistic effect, insecticides adjuvant 5-40%, described plant vinegar liquid is bamboo vinegar, one or more mixtures of pyroligneous liquor or careless vinegar liquid, described plants essential oil is wintergreen, camphor tree oil, caryophyllus oil, peppermint oil, eucalyptus oil, citronella oil, tea oil, one or more mixtures of lemongrass oil or cinnamon oil, described insecticides adjuvant is DBSA, Tween-80, one or more mixtures of Span-80 (SPAN) or OP-10.
Described plants essential oil content is 10-30%, and described plant vinegar liquid content is 40-80%, and described insecticides adjuvant content is 10-40%.
Described plants essential oil content is 20-30%, and described plant vinegar liquid content is 40-80%, and described insecticides adjuvant content is 20-30%.
A manufacture method for multiple-effect nanoscale biopesticide water aqua, is characterized in that first plant vinegar liquid under agitation being added to insecticides adjuvant, stirs completely and dissolves; The in the situation that of continuous stirring, plants essential oil is joined in above-mentioned solution gradually again, till being stirred to the translucent or all-transparent of liquid, form nano level plant essential oil emulsion or molecular level transparency liquid.
Above-mentioned a kind of multiple-effect nanoscale biopesticide water aqua can be in desinsection, expelling parasite and the sterilization of mosquito repellent, family's agricultural, flowers, organic farming and green agriculture, antiviral middle application.
The present invention be take plants essential oil as active component, take plant vinegar liquid as synergist, the agricultural chemicals of manufacture, not only effective, quick-acting is fast, and there is regulating crop growth, desinsection expelling parasite, sterilizing and anti-virus multiple efficacies, and especially take water as solvent, select the insecticides adjuvant that safety is good, the preparation making reaches nanoscale aqueous emulsion or molecular level solubility aqua, safety and environmental protection not only, and due to emulsify well, produce good quick-acting drug effect.Can be used for the non-field Workplaces such as family and office and use, also can in the production for the pollution-free food in agricultural production place, field and organic food, use.
Embodiment
Now, in conjunction with embodiments of the invention and the test of pesticide effectiveness, further illustrate effect of the present invention, the percentage composition relating in present specification is weight percentage.
Embodiment 1
In 1000L reactor, drop into bamboo vinegar 450kg, under constantly stirring, add gradually 25kg emulsifier DBSA, after stirring and dissolving, add plants essential oil wintergreen 25kg, be stirred to transparent till, finished product plants essential oil aqueous emulsion.
Embodiment 2
In 1000L reactor, drop into pyroligneous liquor 400kg, under constantly stirring, adding gradually 40kg emulsifier tween-80, after stirring and dissolving, add plants essential oil camphor tree oil 60kg, be stirred to transparent till, finished product plants essential oil aqueous emulsion.
Embodiment 3
In 1000L reactor, drop into careless vinegar liquid 350kg, under constantly stirring, add gradually 50kg emulsifier Span-80, after stirring and dissolving, add plants essential oil caryophyllus oil 100kg, be stirred to transparent till, finished product plants essential oil aqueous emulsion.
Embodiment 4
In 1000L reactor, drop into plant vinegar liquid 300kg (bamboo vinegar 100kg+ grass vinegar liquid 100kg+ pyroligneous liquor 100kg), under constantly stirring, add gradually 150kg emulsifier op-10, after stirring and dissolving, add plants essential oil Peppermint Oil 50 kg, be stirred to transparent till, finished product plants essential oil aqueous emulsion.
Embodiment 5
In 1000L reactor, drop into bamboo vinegar 250kg, under constantly stirring, add gradually 150kg composite pesticide auxiliary agent (Span-80 75kg+ Tween-80 75kg), after stirring and dissolving, add plants essential oil eucalyptus oil 100kg, be stirred to transparent till, finished product plants essential oil aqueous emulsion.
Embodiment 6
In 1000L reactor, drop into pyroligneous liquor 200kg, under constantly stirring, add gradually 100kg composite pesticide auxiliary agent (Span-80 50kg+ Tween-80 25kg+OP-10 25kg), after stirring and dissolving, add plants essential oil citronella oil 200kg, be stirred to transparent till, finished product plants essential oil aqueous emulsion.
Embodiment 7
In 1000L reactor, drop into careless vinegar liquid 150kg, under constantly stirring, add gradually 200kg composite pesticide auxiliary agent (Span-80 50kg+ Tween-80 50kg+OP-10 100kg), after stirring and dissolving, add plants essential oil tea oil 150kg, be stirred to transparent till, finished product plants essential oil aqueous emulsion.
Embodiment 8
In 1000L reactor, drop into plant vinegar liquid 100kg (bamboo vinegar 50kg+ grass vinegar liquid 25kg+ pyroligneous liquor 25kg), under constantly stirring, add gradually 200kg composite pesticide auxiliary agent (Span-80 50kg+ Tween-80 50kg+OP-10 50kg+ DBSA 50kg), after stirring and dissolving, add plants essential oil 200kg (wintergreen 20kg+ camphor tree oil 30kg+ caryophyllus oil 30kg+ peppermint oil 30kg+ eucalyptus oil 30kg+ citronella oil 30kg+ tea oil 30kg), be stirred to transparent till, finished product plants essential oil aqueous emulsion.
The control agricultural disease effect of above-described embodiment 1-8 is below described by test
The result of the test table of 9 kinds of diseases of table 1 embodiment 1-8 control (preventive effect %, effectively essential oil amount is 500mg/L)
Table 1 shows, by the agricultural disease to a large amount of, carry out screening active ingredients test, can find out that essential oil is not that all agricultural diseases are had to active function, just for wherein seldom a part of disease is effective, and in effective germ, because the different active size differences that show different from crop of disease species are very large, in present specification, disclosed 9 kinds of diseases are all to find that through a large amount of screening tests undocumented is all that control does not have resultful germ with the resultful agriculture germ of this disinfectant use in agriculture control.
Table 2. control tea tree tetranychid result of the test (concentration for the treatment of is essential oil content 750mg/L, proofreaies and correct preventive effect (%))
| Process | After medicine 1 day | After medicine 3 days | After medicine 7 days | After medicine 14 days | After medicine 21 days |
| Embodiment 1 | 32.4 | 48.9 | 80.2 | 92.4 | 80.2 |
| Embodiment 2 | 37.4 | 49.5 | 82.5 | 93.5 | 81.2 |
| Embodiment 3 | 38.5 | 51.0 | 83.6 | 90.1 | 82.5 |
| Embodiment 4 | 41.0 | 52.6 | 85.5 | 87.5 | 80.0 |
| Embodiment 5 | 40.3 | 54.6 | 84.7 | 89.2 | 78.5 |
| Embodiment 6 | 38.5 | 53.2 | 86.9 | 92.5 | 79.4 |
| Embodiment 7 | 39.4 | 55.8 | 88.2 | 94.5 | 85.4 |
| Embodiment 8 | 42.0 | 58.7 | 90.1 | 95.0 | 88.2 |
Table 3. control tealeaves aphid result of the test (concentration for the treatment of is essential oil content 750mg/L)
Table 2-3 data show: embodiment 1-8 all has good effect to control tealeaves aphid, and the control efficiency of embodiment 1-8 does not have significant difference.Embodiment 1-8 extension rate is advisable with activity component concentration 750mg/L.
Table 4 embodiment 1-8 prevents and treats wild cabbage diamond-back moth effect (essential oil concentration 500mg/L)
Table 5 embodiment 1-8 prevents and treats cabbage pieris effect (essential oil concentration 500mg/L)
Table 6 embodiment 1-8 prevents and treats wild cabbage Bemisia tabaci effect (essential oil concentration 500mg/L)
Table 4-6 result of the test show, embodiment 1-8 has higher control efficiency to pest such as Bemisia tabaci, diamond-back moth, cabbage caterpillars at activity component concentration 500mg/L.
Below by test, illustrate that embodiment 1-8 is for sanitary insect pest effect
Test examination worm: culex pipiens fatigans, after sprouting wings, 2-3 days female adults are totally 115; Housefly, the 4th day adult after sprouting wings, totally 128 of male and female half and half; Experimental condition: 26 ± 1 ℃ of temperature, 60 ± 5 ℃ of relative moisture.Simulated field: area 10m
2, height 2.8m, ground white ceramic tiles, ceiling and surroundings wall are sealed transparent glass.
Table 7 embodiment 1-8 knocks down and lethal result of the test culex
| Process | KT50(min) | 24h lethality (%) | 24h rate of knockdown (%) |
| Embodiment 1 | 5.6 | 90.0 | 92.0 |
| Embodiment 2 | 4.6 | 92.5 | 93.4 |
| Embodiment 3 | 4.5 | 93.4 | 95.6 |
| Embodiment 4 | 4.6 | 93.0 | 94.6 |
| Embodiment 5 | 3.5 | 95.6 | 96.5 |
| Embodiment 6 | 3.2 | 96.8 | 98.2 |
| Embodiment 7 | 3.1 | 97.2 | 98.5 |
| Embodiment 8 | 1.9 | 99.4 | 99.8 |
Table 8 embodiment 1-8 knocks down and lethal result of the test housefly
| Process | KT50(min) | 24h lethality (%) | 24h rate of knockdown (%) |
| Embodiment 1 | 4.5 | 82.5 | 85.6 |
| Embodiment 2 | 4.2 | 85.6 | 87.9 |
| Embodiment 3 | 4.0 | 86.6 | 88.9 |
| Embodiment 4 | 4.1 | 87.8 | 89.8 |
| Embodiment 5 | 3.5 | 89.9 | 92.4 |
| Embodiment 6 | 3.2 | 92.2 | 95.6 |
| Embodiment 7 | 3.0 | 93.4 | 96.5 |
| Embodiment 8 | 3.0 | 98.4 | 98.8 |
Table 7-8 shows, embodiment 1-8 all has a good effect to knocking down with lethal of culex and housefly.
The beneficial effect of above-mentioned female mosquito olfaction disrupter composition is below described by test.
Test 1: test method detects mosquito density with reference to GB13917.8-1992 field condition, adopts people to lure method investigation mosquito density.In each experiment room, arrange 2 fixing investigators (determine people, fixed point, regularly), experimenter has drawn 2 trouser legs to knee and has exposed skin, with flashlight lighting, 45min catches and stops dropping on the mosquito on exposed leg with electric mosquito-suction device continuously, identifies subsequently and record quantity, sex and the kind of mosquito.Mosquito density rate of descent (%)=1-experiment mosquito density/blank room, room mosquito density.Result of the test is in Table 9.
As seen from Table 9, after the product that use embodiment 1-7 makes, mosquito density obviously declines to a great extent, and mosquito density rate of descent all reaches more than 80%, and some is up to more than 90%.
Test 2: test method is with reference to GB13917.8-1992; the Culex quinquefasciatus of use laboratory and field-collected strains tests and on-the-spot people lures biting test; with reference to GB13917.4-92, adopt the field-collected strains Culex quinquefasciatus worm of collection in worksite to measure with on-the-spot people and lure method observation bite by mosquitos number and embodiment 1-8 to make the protection effect of product at the rear 2-3d of laboratory rearing emergence.Mosquito density rate of descent (%)=1-experiment mosquito density/blank room, room mosquito density.
Test 3: subjects is 20 people, on average everyone is by mosquito bite 5 times, totally 1 ((person-time, after mosquito bite, use immediately the product spraying that embodiment 1-7 makes to smear affected part 5cm diameter range, and start timing, the detumescence person-time after antipruritic person-time and 10 minutes of adding up respectively 1 minute, computational methods: antipruritic percentage=antipruritic person-time/total statistics person-time * 100; Detumescence percentage=detumescence person-time/always add up person-time * 100.
Table 9 embodiment 1-8 is bitten effect and antipruritic effect to mosquito density rate of descent, prevention
From table 9 result, use after the product that embodiment 1-8 makes mosquito density rate of descent all over 85%, illustrate that repelling effect is good; Meanwhile, bite by mosquitos number of times significantly reduces, and protection effect all reaches more than 90%, and especially the protection effect of embodiment 8 reaches more than 99%.The product spraying that use embodiment 1-8 makes is smeared antipruritic effect after 1 minute and is surpassed 60%, 10 minute detumescence effect all over 63%.
Claims (3)
1. a multiple-effect nano grade biological pesticide emulsion in water, it is characterized in that the pesticide activity component plants essential oil 200kg by weight portion meter, the plant vinegar liquid 100kg with synergistic effect, composite pesticide auxiliary agent 200kg forms, described plant vinegar liquid is by bamboo vinegar 50kg, grass vinegar liquid 25kg, pyroligneous liquor 25kg mixes, described composite pesticide auxiliary agent is by Span-80 50kg, Tween-80 50kg, OP-10 50kg, DBSA 50kg mixes, described plants essential oil is by wintergreen 20kg, camphor tree oil 30kg, caryophyllus oil 30kg, peppermint oil 30kg, eucalyptus oil 30k, citronella oil 30kg, tea oil 30kg forms.
2. a preparation method for multiple-effect nano grade biological pesticide emulsion in water according to claim 1, is characterized in that first plant vinegar liquid under agitation being added to composite pesticide auxiliary agent, stirs completely and dissolves; The in the situation that of continuous stirring, plants essential oil is joined in above-mentioned solution gradually again, till being stirred to the translucent or all-transparent of liquid, form nano level plant essential oil emulsion or molecular level transparency liquid.
3. a purposes for multiple-effect nano grade biological pesticide emulsion in water according to claim 1, is characterized in that desinsection, expelling parasite and sterilization in mosquito repellent, family's agricultural, flowers, organic farming and green agriculture, the application in antiviral.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110315898.8A CN102524319B (en) | 2011-10-18 | 2011-10-18 | Multiple-effect nanoscale biopesticide water aqua and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110315898.8A CN102524319B (en) | 2011-10-18 | 2011-10-18 | Multiple-effect nanoscale biopesticide water aqua and use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102524319A CN102524319A (en) | 2012-07-04 |
| CN102524319B true CN102524319B (en) | 2014-03-26 |
Family
ID=46333364
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110315898.8A Expired - Fee Related CN102524319B (en) | 2011-10-18 | 2011-10-18 | Multiple-effect nanoscale biopesticide water aqua and use thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102524319B (en) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103494886B (en) * | 2013-08-19 | 2015-04-08 | 浙江农林大学 | Spray type beriberi liquid containing vinegar liquid and essential oil of bamboo and wood, nano-grade manufacturing method, and applications thereof |
| CN103444798B (en) * | 2013-08-19 | 2016-07-06 | 浙江农林大学 | A kind of safe anophelifuge containing bamboo wood vinegar and manufacture method thereof and purposes |
| CN103493854B (en) * | 2013-08-19 | 2016-06-08 | 浙江农林大学 | A kind of plant's Environmental Health preparation containing bamboo wood vinegar and quintessence oil and manufacture method thereof and purposes |
| CN104068069A (en) * | 2014-06-27 | 2014-10-01 | 何芳 | Multi-effect bio-pesticide aqueous solution |
| CN104068062B (en) * | 2014-07-17 | 2016-04-20 | 湖南省畜牧兽医研究所 | Chlorine tetramethrin aqueous emulsion of a kind of repelling and killing bugs and preparation method thereof |
| CN104604939A (en) * | 2015-01-08 | 2015-05-13 | 馥稷生物科技发展（上海）有限公司 | Pesticide composite containing sophocarpidine |
| CN104920492A (en) * | 2015-06-12 | 2015-09-23 | 福建省德盛生物工程有限责任公司 | Low-volume spray containing eucalyptol |
| CN105594765A (en) * | 2015-12-26 | 2016-05-25 | 郑州金土地能源科技有限公司 | Environment-friendly pesticide |
| CN105594692A (en) * | 2015-12-29 | 2016-05-25 | 辛集市远翔环保能源科技有限公司 | Pesticide synergist |
| CN105961464A (en) * | 2016-06-01 | 2016-09-28 | 蚌埠市宝煦家庭农场 | Insecticide for preventing and treating Yali pear Psylla chinensis insect damage |
| CN106962411A (en) * | 2017-04-07 | 2017-07-21 | 安徽中煦环保新材料科技有限公司 | A kind of flowers environmental protection nursing material and preparation method thereof |
| CN109303030A (en) * | 2017-07-29 | 2019-02-05 | 安吉县正新牧业有限公司 | A kind of bamboo element environmental improvement agent |
| CN108244150A (en) * | 2017-12-18 | 2018-07-06 | 广州美林生态科技有限公司 | Novel crops insect prevention, mould proof, expelling parasite lotion and its preparation method and application |
| CN111066820A (en) * | 2018-10-22 | 2020-04-28 | 深圳市能迩环保科技实业发展有限公司 | Safe mosquito repellent containing wood vinegar and preparation method thereof |
| CN109673675A (en) * | 2018-12-29 | 2019-04-26 | 广西必要科技有限公司 | A kind of tea oil and the agricultural synergist of cassia twig oil and its preparation method and application |
| CN110326629A (en) * | 2019-07-01 | 2019-10-15 | 河北上瑞生物科技有限公司 | A kind of agricultural essential oil auxiliary agent and its preparation method and application |
| CN116849215B (en) * | 2023-06-19 | 2024-06-11 | 浙江大学 | Application of methyl salicylate in inhibiting development of secondary wall of citrus juice cells |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101297942A (en) * | 2008-06-25 | 2008-11-05 | 浙江林学院 | Itch-reliving detumescence agent containing plant vinegar liquid and preparation thereof |
| CN101297644A (en) * | 2008-06-25 | 2008-11-05 | 浙江林学院 | Mosquito repellent incense containing plant vinegar liquid and use thereof |
| CN101433730A (en) * | 2008-11-28 | 2009-05-20 | 浙江林学院 | Treatment agent for domestic refuse containing plant vinegar liquor and uses thereof |
-
2011
- 2011-10-18 CN CN201110315898.8A patent/CN102524319B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101297942A (en) * | 2008-06-25 | 2008-11-05 | 浙江林学院 | Itch-reliving detumescence agent containing plant vinegar liquid and preparation thereof |
| CN101297644A (en) * | 2008-06-25 | 2008-11-05 | 浙江林学院 | Mosquito repellent incense containing plant vinegar liquid and use thereof |
| CN101433730A (en) * | 2008-11-28 | 2009-05-20 | 浙江林学院 | Treatment agent for domestic refuse containing plant vinegar liquor and uses thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102524319A (en) | 2012-07-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102524319B (en) | Multiple-effect nanoscale biopesticide water aqua and use thereof | |
| CN102369957B (en) | Multi-effect biological pesticide aqueous solution agent and preparation method and application thereof | |
| CN102428973B (en) | Agricultural pesticide with essential oil and application thereof | |
| CN104068069A (en) | Multi-effect bio-pesticide aqueous solution | |
| BR112020003643A2 (en) | miticide and concrete application | |
| CN107258826A (en) | It is a kind of to reduce the biological insecticides of Cucumber Pests And Diseases | |
| CN101697732B (en) | Sanitary pesticide composition | |
| US8414934B2 (en) | Compositions and methods for arachnid control | |
| KR101400713B1 (en) | Composition of pesticide containing pesticidal essential oils and/or food additives | |
| CN102726396B (en) | A kind of spinosad microemulsion and preparation method thereof | |
| KR101632246B1 (en) | Pesticides using the Insect flower powder or sludge and manufacture method thereof | |
| CN108684740A (en) | Environment protection type insecticidal spirit | |
| KR20140084596A (en) | Pesticides using the Insect flower powder or sludge | |
| CN102988462B (en) | Chinese medicinal preparation for controlling parasites of aquatic animal as well as preparation method and application thereof | |
| CN102726443B (en) | Ivermectin and dipterex compound pesticide | |
| CN103315008B (en) | Preparation method of Suaeda salsa used as agricultural pesticide and application | |
| CN102308837A (en) | Insecticidal composition | |
| CN106259379B (en) | A kind of nematicidal composition containing trifluorobutene base class compound | |
| CN104222083A (en) | Monomorium pharaonis repellent containing citronellal 1,3-dipropylacetal | |
| CN104351218B (en) | Microcapsule suspension containing nimbin and chitosan oligosaccharide as effective components | |
| CN104472519A (en) | Anti-mite composition | |
| CN107711836A (en) | A kind of wasp fly traps composition and its application | |
| Ali et al. | Effects of Nerium oleander extract on scaly leg mites (Knemidocoptes mutans) in back yard chickens. | |
| CN104304285B (en) | Compound insecticidal water emulsion agent for preventing and treating contariniapyrivora | |
| CN104430602A (en) | Bactericidal and antiviral bio-pesticide aqueous solution |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140326 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |