CN109731132B - Liquid bandage containing rhizoma Zingiberis recens extract for relieving arthralgia - Google Patents
Liquid bandage containing rhizoma Zingiberis recens extract for relieving arthralgia Download PDFInfo
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Abstract
The invention discloses a ginger extract-containing liquid bandage for relieving arthralgia, which comprises the following raw materials in parts by weight: 2 parts of ginger extract, 1 part of traditional Chinese medicine extract, 1 part of peach tree oil extract, 2 parts of elm bark extract, 10 parts of chitosan, 30 parts of cellulose acetate, 5 parts of polyvinylpyrrolidone and 100 parts of ethanol solution with the mass fraction of 95%; wherein the pH of the ethanol solution is adjusted to 8-9 by using a boric acid buffer solution with the pH of 9.5. The liquid bandage containing the ginger extract for relieving the arthralgia disclosed by the invention not only has the effect of effectively relieving the arthralgia, but also can be used for quickly forming a film on the surface of skin.
Description
Technical Field
The invention relates to the field of preparation of ginger extracts. More particularly, the present invention relates to a liquid bandage for relieving joint pain containing ginger extract.
Background
Joint pain is a systemic autoimmune disease characterized by arthritis, is one of chronic diseases seriously threatening health, and is mainly characterized by chronic, symmetrical, progressive and invasive properties, particularly manifested as joint swelling and deformation and bone destruction, even disability, caused by joint synovitis.
At present, most of arthritis treatment is traditional Chinese medicine ointment or patch, the traditional Chinese medicine ointment is easily erased when being applied to a diseased part, the effect of the ointment is influenced, clothes are easily dirtied, the ventilation property of skin is influenced when the patch is applied to the position, and symptoms such as skin allergy and the like easily appear at the bonded part.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and to provide at least the advantages described later.
It is still another object of the present invention to provide a liquid bandage for relieving joint pain containing ginger extract, which not only has an effect of effectively relieving joint pain, but also can rapidly form a film on the skin surface.
To achieve these objects and other advantages in accordance with the present invention, there is provided a liquid bandage for relieving joint pain comprising ginger extract, characterized by comprising the following raw materials in parts by weight: 2 parts of ginger extract, 1 part of traditional Chinese medicine extract, 1 part of peach tree oil extract, 2 parts of elm bark extract, 10 parts of chitosan, 30 parts of cellulose acetate, 5 parts of polyvinylpyrrolidone and 100 parts of ethanol solution with the mass fraction of 95%;
wherein the pH of the ethanol solution is adjusted to 8-9 by using a boric acid buffer solution with the pH of 9.5;
the preparation method of the traditional Chinese medicine extract comprises the following steps: a1, mixing and crushing dysosma versipellis, radix angelicae, fel Ursi, radix clematidis, radix aconiti, rhizoma arisaematis, Syngnathus and fructus gardeniae in a mass ratio of 1:1:1.3:1.8:2: 2.5:3, and sieving to obtain crushed materials; a2, mixing a 95% methanol solution, 5 times acetone, 1 time N, N-dimethylformamide and 0.01 time dimethyl sulfoxide which are 10 times of the total mass of the crushed materials, adding 10% citric acid and 0.01 time acetic anhydride which are 0.1 time of the total mass of the crushed materials, and stirring for 20min to obtain a standby solution; a3, adding the crushed material into the standby liquid, heating for 10 hours at 50 ℃, filtering and concentrating to obtain a concentrate, carrying out column chromatography on the concentrate, wherein an elution system is an ethyl acetate-methanol system, collecting eluent with the volume ratio of ethyl acetate-methanol being 6-4: 1, and concentrating to obtain the traditional Chinese medicine extract.
Preferably, the preparation method of the ginger extract comprises the following steps:
s1, drying the ginger at 60 ℃, and crushing to obtain ginger powder;
s2, taking tetrahydrofuran which is 10 times of the total mass of the ginger powder, adding fumed silica which is 5 times of the total mass of the ginger powder into the tetrahydrofuran, stirring for 10min under the conditions of 50 ℃ and 400r/min, then dropwise adding 4M sulfuric acid solution which is 1 time of the total mass of the ginger powder under the conditions of 60 ℃ and 400r/min, continuing stirring for 20min after the sulfuric acid solution is dropwise added, then adding silane coupling agent which is 6 times of the total mass of the ginger powder seven times, stirring for 1h under the conditions of 70 ℃ and 400r/min, filtering to obtain solid, finally flushing the solid with ethanol solution with the mass fraction of 95%, and drying the solid for 6h under the condition of 60 ℃ to obtain the modified fumed silica, wherein the silane coupling agent is (HO)3Si(CH2)3N(CH2PO3H2)2；
S3, taking a solvent with the weight of 20 times of the total weight of ginger powder, adding acetic acid into the solvent, adjusting the pH value of the solvent to 5-6, adding modified fumed silica, stirring at 50 ℃ for 20min at 400r/min, adding ginger powder, performing reflux extraction, filtering to obtain a first filtrate and a filter residue, adding methanol with the weight of 4 times of the total weight of the filter residue into the filter residue, soaking for 15h, filtering to obtain a second filtrate, combining and concentrating the first filtrate and the second filtrate to obtain an extract, separating the extract by column chromatography, wherein the elution system is a petroleum ether-ethyl acetate system, a petroleum ether-dichloromethane system, a petroleum ether-methanol system and a petroleum ether-acetone system, and the eluents with the volume ratio of 6:1 and 8:1 are collected, Eluting with petroleum ether-methanol at volume ratio of 7:1 and eluting with petroleum ether-acetone at volume ratio of 2:1, mixing eluates, and concentrating to obtain rhizoma Zingiberis recens extract.
Preferably, the preparation method of the peach tree oil extract comprises the following steps: adding 10 times of water and 5 times of methanol into peach tree oil, heating at 60 deg.C for 5 hr, and filtering to obtain a third filtrate; adding active carbon with the amount of 0.5 times of the total mass of the third filtrate into the third filtrate for decolorization, and filtering to obtain a fourth filtrate; adding white sugar 0.08 times the total weight of the fourth filtrate, heating at 80 deg.C for 1 hr, and concentrating to obtain peach tree oil extract.
Preferably, the preparation method of the elm bark extract comprises the following steps: drying cortex Ulmi Pumilae in the sun, pulverizing, and sieving to obtain cortex Ulmi Pumilae powder; adding water in an amount which is 20 times of the total mass of the elm bark powder and methanol in an amount which is 10 times of the total mass of the elm bark powder, mixing, heating for 5 hours at 70 ℃, and filtering to obtain a fifth filtrate; adding 0.5 times of active carbon into the fifth filtrate for decolorizing, filtering, and concentrating to obtain cortex Ulmi Pumilae extract.
Preferably, the solvent is ethyl acetate.
Preferably, in S1, the ginger is dried at 60 ℃, and after being crushed, the ginger is further sieved to obtain 200-mesh ginger powder.
The invention at least comprises the following beneficial effects:
firstly, the invention not only has the function of effectively relieving the joint pain, but also can quickly form a film on the surface of the skin and prolong the action time.
Secondly, adding Chinese herbal extracts, namely dysosma versipellis, angelica dahurica, bear gall, radix clematidis, radix aconiti, rhizoma arisaematis, syngnathus and cape jasmine, which are helpful for relieving arthralgia; in the preparation method of the traditional Chinese medicine extract, more active ingredients can be extracted by adding a methanol solution, acetone and a mixed solvent of N, N-dimethylformamide for extraction, so that the effect of the liquid bandage on relieving arthralgia is enhanced; the addition of dimethyl sulfoxide, citric acid and acetic anhydride can improve the permeability of cell walls and release more effective components in cells, and compared with the technology of extracting with water, the preparation method provided by the invention contains more effective components and has a better effect of relieving arthralgia.
Thirdly, the ginger extract is added, and the ginger extract and the traditional Chinese medicine extract are used together, so that the arthralgia can be effectively relieved; adding modified fumed silica into the solvent to make ginger powder and fumed silica generate friction during cold soaking extraction or reflux extraction of ginger powder, so as to improve cell wall breaking rate and promote extraction of effective components; the addition of sulfuric acid facilitates the formation of silicon hydroxyl on the surface of the fumed silica, promotes the formation of stable silicon-oxygen-silicon bonds between the fumed silica and the silane coupling agent, and the addition of the silane coupling agent in equal amount for multiple times can reduce the condensation probability of the silane coupling agent and improve the utilization rate of the silane coupling agent; use (HO)3Si(CH2)3N(CH2PO3H2)2The fumed silica is modified, so that on one hand, the dispersibility of the fumed silica in a solvent can be improved, the contact between ginger powder and the fumed silica is increased, and the cell wall breaking rate is improved, on the other hand, the fumed silica is modified by using a silane coupling agent, the adsorption of the fumed silica to effective ingredients can be reduced, the separation of the effective ingredients from the fumed silica is facilitated, and the extraction rate is improved; the ethanol solution is used for washing, so that unreacted silane coupling agent, self-condensed silane coupling agent and fumed silica can be separated conveniently, and new impurities introduced into effective components are avoided; the effective components in the ginger extract can be effectively improved by using a plurality of elution systems for elution.
Fourthly, the peach tree oil is added, so that the surface tension of the liquid bandage can be reduced, the liquid flowing on the skin is reduced, the bonding force of the liquid bandage is improved, and the effect of relieving the arthralgia is further effectively improved; extracting with 10 times of water and 5 times of methanol mixed solvent to extract effective components of peach tree oil; the white sugar and the peach tree oil act to improve the close fit between the liquid bandage and the skin.
Fifthly, the elm bark extract is added, and the elm bark extract and the traditional Chinese medicine extract are used together, so that the arthralgia can be effectively relieved; the mixed solvent of 20 times of water and 10 times of methanol is used to facilitate the release of the effective components of elm bark.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
< example 1>
The liquid bandage containing ginger extract for relieving arthralgia comprises the following raw materials in parts by weight: 2 parts of ginger extract, 1 part of traditional Chinese medicine extract, 1 part of peach tree oil extract, 2 parts of elm bark extract, 10 parts of chitosan, 30 parts of cellulose acetate, 5 parts of polyvinylpyrrolidone and 100 parts of ethanol solution with the mass fraction of 95%;
wherein the pH of the ethanol solution is adjusted to 8-9 by using a boric acid buffer solution with the pH of 9.5;
the preparation method of the traditional Chinese medicine extract comprises the following steps: a1, mixing and crushing dysosma versipellis, radix angelicae, fel Ursi, radix clematidis, radix aconiti, rhizoma arisaematis, Syngnathus and fructus gardeniae in a mass ratio of 1:1:1.3:1.8:2: 2.5:3, and sieving to obtain crushed materials; a2, mixing a 95% methanol solution, 5 times acetone, 1 time N, N-dimethylformamide and 0.01 time dimethyl sulfoxide which are 10 times of the total mass of the crushed materials, adding 10% citric acid and 0.01 time acetic anhydride which are 0.1 time of the total mass of the crushed materials, and stirring for 20min to obtain a standby solution; a3, adding the crushed material into the standby liquid, heating for 10 hours at 50 ℃, filtering and concentrating to obtain a concentrate, carrying out column chromatography on the concentrate, wherein an elution system is an ethyl acetate-methanol system, collecting eluent with the volume ratio of ethyl acetate-methanol being 6-4: 1, and concentrating to obtain the traditional Chinese medicine extract.
The preparation method of the ginger extract comprises the following steps:
s1, drying the ginger at 60 ℃, and crushing to obtain ginger powder;
s2, taking gingerTetrahydrofuran with the total weight of 10 times of the powder, then adding fumed silica with the total weight of 5 times of the ginger powder into the tetrahydrofuran, stirring for 10min under the conditions of 50 ℃ and 400r/min, then dropwise adding 4M sulfuric acid solution with the total weight of 1 time of the ginger powder under the conditions of 60 ℃ and 400r/min, continuing stirring for 20min after the sulfuric acid solution is dropwise added, then adding silane coupling agent with the total weight of 6 times of the ginger powder in seven times in equal amount, stirring for 1h under the conditions of 70 ℃ and 400r/min, filtering to obtain solid, finally washing the solid with ethanol solution with the mass fraction of 95%, and drying the solid for 6h under the condition of 60 ℃ to obtain the modified fumed silica, wherein the silane coupling agent is (HO)3Si(CH2)3N(CH2PO3H2)2；
S3, taking a solvent with the weight of 20 times of the total weight of ginger powder, adding acetic acid into the solvent, adjusting the pH value of the solvent to 5-6, adding modified fumed silica, stirring at 50 ℃ for 20min at 400r/min, adding ginger powder, performing reflux extraction, filtering to obtain a first filtrate and a filter residue, adding methanol with the weight of 4 times of the total weight of the filter residue into the filter residue, soaking for 15h, filtering to obtain a second filtrate, combining and concentrating the first filtrate and the second filtrate to obtain an extract, separating the extract by column chromatography, wherein the elution system is a petroleum ether-ethyl acetate system, a petroleum ether-dichloromethane system, a petroleum ether-methanol system and a petroleum ether-acetone system, and the eluents with the volume ratio of 6:1 and 8:1 are collected, Eluting with petroleum ether-methanol at volume ratio of 7:1 and eluting with petroleum ether-acetone at volume ratio of 2:1, mixing eluates, and concentrating to obtain rhizoma Zingiberis recens extract.
The preparation method of the peach tree oil extract comprises the following steps: adding 10 times of water and 5 times of methanol into peach tree oil, heating at 60 deg.C for 5 hr, and filtering to obtain a third filtrate; adding active carbon with the amount of 0.5 times of the total mass of the third filtrate into the third filtrate for decolorization, and filtering to obtain a fourth filtrate; adding white sugar 0.08 times the total weight of the fourth filtrate, heating at 80 deg.C for 1 hr, and concentrating to obtain peach tree oil extract.
The preparation method of the elm bark extract comprises the following steps: drying cortex Ulmi Pumilae in the sun, pulverizing, and sieving to obtain cortex Ulmi Pumilae powder; adding water in an amount which is 20 times of the total mass of the elm bark powder and methanol in an amount which is 10 times of the total mass of the elm bark powder, mixing, heating for 5 hours at 70 ℃, and filtering to obtain a fifth filtrate; adding 0.5 times of active carbon into the fifth filtrate for decolorizing, filtering, and concentrating to obtain cortex Ulmi Pumilae extract.
The solvent is ethyl acetate.
S1, drying ginger at 60 ℃, crushing and sieving to obtain 200-mesh ginger powder.
< comparative example 1>
Adopts the existing spray for treating arthritis.
< comparative example 2>
A liquid bandage containing ginger extract for relieving joint pain is prepared according to the method of example 1, wherein the difference is the preparation method of the traditional Chinese medicine extract, and the preparation method specifically comprises the following steps: mixing and crushing dysosma versipellis, radix angelicae, fel Ursi, radix clematidis, radix aconiti, rhizoma arisaematis, syngnathus and gardenia jasminoides in a mass ratio of 1:1:1.3:1.8:2: 2.5:3, sieving to obtain crushed materials, adding water in an amount which is 20 times of the total mass ratio of the crushed materials into the crushed materials, heating for 10 hours at 100 ℃, filtering and concentrating to obtain a concentrate, performing column chromatography on the concentrate, wherein an elution system is an ethyl acetate-methanol system, collecting eluent with a volume ratio of ethyl acetate-methanol of 6-4: 1, and concentrating to obtain the traditional Chinese medicine extract.
< comparative example 3>
A liquid bandage for relieving joint pain containing ginger extract was prepared according to the method of example 1, except that the ginger extract was prepared by a different method, specifically: drying ginger at 60 ℃, crushing to obtain ginger powder, adding ethyl acetate 20 times of the total mass of the ginger powder into the ginger powder, carrying out reflux extraction, filtering and concentrating to obtain an extract, separating the extract by adopting column chromatography, wherein an elution system comprises an eluent with a petroleum ether-ethyl acetate volume ratio of 6:1, an eluent with a petroleum ether-dichloromethane volume ratio of 8:1, an eluent with a petroleum ether-methanol volume ratio of 7:1 and an eluent with a petroleum ether-acetone volume ratio of 2:1, combining the eluents and concentrating to obtain the ginger extract.
< comparative example 4>
A liquid bandage for relieving joint pain containing ginger extract was prepared according to the method of example 1, except that peach oil extract was not added.
< comparative example 5>
A liquid bandage for relieving joint pain containing ginger extract was prepared according to the method of example 1, except that the elm bark extract was not added.
< comparative example 6>
A liquid bandage for relieving arthralgia containing ginger extract was prepared according to the method of example 1, except that fumed silica was not used (HO)3Si(CH2)3N(CH2PO3H2)2And (4) carrying out modification.
< experimental characterization > 7 groups of liquid bandages for relieving joint pain containing ginger extract were prepared according to the methods of example 1 and comparative examples 1 to 6, and used in the following studies, respectively.
1. Mouse experiment
1.1 preparation of arthritis mouse model
70 male mice weighing 80-100 g were taken and randomly divided into 7 groups of 10 mice each. After mice were fully anesthetized with 10% chloral hydrate (0.3mL/100g) in the abdominal cavity (no blinking reaction, no pain when pinching the feet), the right hind toes were sterilized with 75% alcohol and injected with 0.2mL carrageenan.
1.2 swelling assessment
The volume of swelling of the right hind paw (in mL) of each mouse was measured using a mouse toe volume meter to two decimal places. The foot swelling rate, measured after 2, 4, 6, 24, 48 hours after carrageenan injection, is the percent difference in foot volume and is calculated from the following equation: the results are shown in table 1, wherein one of 7 groups of mice was a model group (without drug) and the other 6 groups were sprayed with the liquid bandages of example 1 and comparative examples 1 to 5 (with drug after carrageenan injection) one by one.
The results are expressed as mean values, and p <0.05 by statistical analysis, is statistically significant.
TABLE 1 percent change after carrageenan injection and after treatment
| Group of | 2h | 4h | 6h | 24h | 48h |
| Model set | 17.20 | 54.1627 | 85.4531 | 73.656 | 62.775 |
| Example 1 | 7.71 | 19.2612 | 38.2319 | 23.3515 | 10.0113 |
| Comparative example 1 | 9.29 | 28.4819 | 54.16226 | 46.248 | 39.797 |
| Comparative example 2 | 14.39 | 30.6216 | 53.52323 | 39.8514 | 28.7311 |
| Comparative example 3 | 13.68 | 22.4415 | 43.0921 | 32.6211 | 23.929 |
| Comparative example 4 | 8.75 | 21.6814 | 43.0522 | 31.7712 | 19.4512 |
| Comparative example 5 | 16.45 | 36.4918 | 60.7124 | 50.3510 | 42.568 |
Comparative analysis example 1 and comparative example 1 show that the present invention can effectively relieve joint pain;
comparative analysis examples 1 and 2 show that the addition of the ginger extract and the combined use of the ginger extract and the traditional Chinese medicine extract can effectively relieve joint pain, and meanwhile, the preparation method of the ginger extract can enable the ginger extract to contain various effective components;
comparative analysis example 1, comparative example 3, and comparative example 4 show that the addition of peach tree oil extract and elm extract can further improve the effect of alleviating joint pain.
2. Film Forming Effect
Cleaning pig ears with cold water, removing hair, collecting pig ears with thickness of about 600 μm with skin grafting knife, and storing at-20 deg.C; the liquid bandages of example 1 and comparative example 3 were sprayed on the ears of pigs, and the film forming time of the liquid bandages on the ears of pigs and the surface tension of the liquid bandages were observed, and the results are shown in table 2;
TABLE 2 film formation time and surface tension
| Group of | Film formation time | Surface tension (mN/m) |
| Example 1 | 8s | 20.619±0.027 |
| Comparative example 4 | 13s | 26.785±0.018 |
The result shows that the surface tension of the liquid bandage can be reduced by adding the peach tree oil extract, the liquid flowing on the skin is reduced, the adhesive force of the liquid bandage is improved, and the effect of relieving the arthralgia is further effectively improved.
3. Cell wall breaking rate
Observing the cell wall breaking condition of 200-mesh ginger powder under a microscope, and simultaneously observing the wall breaking conditions of the extracts corresponding to example 1 and comparative example 3, wherein the results are shown in table 3;
TABLE 3 cell wall-breaking Rate
| Group of | Cell wall breaking rate |
| Example 1 | 88％ |
| Ginger powder | 79％ |
| Comparative example 3 | 84％ |
| Comparative example 6 | 81％ |
The result shows that the friction of the fumed silica on the ginger powder is favorable for promoting the cell wall breaking in the extraction process by adding the modified fumed silica.
4. Settling of fumed silica
Observing the dispersion condition of the fumed silica in the ethyl acetate in the extraction process, wherein the result is shown in table 4;
TABLE 4 layering situation
| Group of | Layered situation |
| Example 1 | Uniform dispersion of fumed silica |
| Comparative example 6 | Sinking of fumed silica |
The results show the use (HO)3Si(CH2)3N(CH2PO3H2)2The modification of the fumed silica can improve the dispersibility of the fumed silica in a solvent, increase the contact between ginger powder and the fumed silica and improve the wall-breaking rate.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the embodiments shown and described without departing from the generic concept as defined by the claims and their equivalents.
Claims (6)
1. The liquid bandage containing ginger extract for relieving arthralgia is characterized by comprising the following raw materials in parts by weight: 2 parts of ginger extract, 1 part of traditional Chinese medicine extract, 1 part of peach tree oil extract, 2 parts of elm bark extract, 10 parts of chitosan, 30 parts of cellulose acetate, 5 parts of polyvinylpyrrolidone and 100 parts of ethanol solution with the mass fraction of 95%;
wherein the pH of the ethanol solution is adjusted to 8-9 by using a boric acid buffer solution with the pH of 9.5;
the preparation method of the traditional Chinese medicine extract comprises the following steps: a1, mixing and crushing dysosma versipellis, radix angelicae, fel Ursi, radix clematidis, radix aconiti, rhizoma arisaematis, Syngnathus and fructus gardeniae in a mass ratio of 1:1:1.3:1.8:2: 2.5:3, and sieving to obtain crushed materials; a2, mixing a 95% methanol solution, 5 times acetone, 1 time N, N-dimethylformamide and 0.01 time dimethyl sulfoxide which are 10 times of the total mass of the crushed materials, adding 10% citric acid and 0.01 time acetic anhydride which are 0.1 time of the total mass of the crushed materials, and stirring for 20min to obtain a standby solution; a3, adding the crushed material into the standby liquid, heating for 10 hours at 50 ℃, filtering and concentrating to obtain a concentrate, carrying out column chromatography on the concentrate, wherein an elution system is an ethyl acetate-methanol system, collecting eluent with the volume ratio of ethyl acetate-methanol being 6-4: 1, and concentrating to obtain the traditional Chinese medicine extract.
2. The liquid bandage for relieving joint pain comprising ginger extract as claimed in claim 1, wherein the ginger extract is prepared by a method comprising:
s1, drying the ginger at 60 ℃, and crushing to obtain ginger powder;
s2, taking tetrahydrofuran which is 10 times of the total mass of the ginger powder, adding fumed silica which is 5 times of the total mass of the ginger powder into the tetrahydrofuran, stirring for 10min under the conditions of 50 ℃ and 400r/min, then dropwise adding 4M sulfuric acid solution which is 1 time of the total mass of the ginger powder under the conditions of 60 ℃ and 400r/min, continuing stirring for 20min after the sulfuric acid solution is dropwise added, then adding silane coupling agent which is 6 times of the total mass of the ginger powder seven times, stirring for 1h under the conditions of 70 ℃ and 400r/min, filtering to obtain solid, finally flushing the solid with ethanol solution with the mass fraction of 95%, and drying the solid for 6h under the condition of 60 ℃ to obtain the modified fumed silica, wherein the silane coupling agent is (HO)3Si(CH2)3N(CH2PO3H2)2；
S3, taking a solvent with the weight of 20 times of the total weight of ginger powder, adding acetic acid into the solvent, adjusting the pH value of the solvent to 5-6, adding modified fumed silica, stirring at 50 ℃ for 20min at 400r/min, adding ginger powder, performing reflux extraction, filtering to obtain a first filtrate and a filter residue, adding methanol with the weight of 4 times of the total weight of the filter residue into the filter residue, soaking for 15h, filtering to obtain a second filtrate, combining and concentrating the first filtrate and the second filtrate to obtain an extract, separating the extract by column chromatography, wherein the elution system is a petroleum ether-ethyl acetate system, a petroleum ether-dichloromethane system, a petroleum ether-methanol system and a petroleum ether-acetone system, and the eluents with the volume ratio of 6:1 and 8:1 are collected, Eluting with petroleum ether-methanol at volume ratio of 7:1 and eluting with petroleum ether-acetone at volume ratio of 2:1, mixing eluates, and concentrating to obtain rhizoma Zingiberis recens extract.
3. The liquid bandage for relieving joint pain containing ginger extract as claimed in claim 1, wherein the peach tree oil extract is prepared by the method comprising: adding 10 times of water and 5 times of methanol into peach tree oil, heating at 60 deg.C for 5 hr, and filtering to obtain a third filtrate; adding active carbon with the amount of 0.5 times of the total mass of the third filtrate into the third filtrate for decolorization, and filtering to obtain a fourth filtrate; adding white sugar 0.08 times the total weight of the fourth filtrate, heating at 80 deg.C for 1 hr, and concentrating to obtain peach tree oil extract.
4. The liquid bandage for relieving joint pain containing ginger extract as claimed in claim 1, wherein the elm bark extract is prepared by the method comprising: drying cortex Ulmi Pumilae in the sun, pulverizing, and sieving to obtain cortex Ulmi Pumilae powder; adding water in an amount which is 20 times of the total mass of the elm bark powder and methanol in an amount which is 10 times of the total mass of the elm bark powder, mixing, heating for 5 hours at 70 ℃, and filtering to obtain a fifth filtrate; adding 0.5 times of active carbon into the fifth filtrate for decolorizing, filtering, and concentrating to obtain cortex Ulmi Pumilae extract.
5. The liquid bandage for relieving joint pain comprising ginger extract as claimed in claim 2 wherein the vehicle is ethyl acetate.
6. The liquid bandage for relieving joint pain containing ginger extract as claimed in claim 2, wherein in S1, ginger is dried at 60 ℃, and after being pulverized, it is further sieved to obtain 200 mesh ginger powder.
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