CN109007439A - Containing callicarpa nudiflora prawn feed additive and its preparation method and application - Google Patents
Containing callicarpa nudiflora prawn feed additive and its preparation method and application Download PDFInfo
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- CN109007439A CN109007439A CN201810826549.4A CN201810826549A CN109007439A CN 109007439 A CN109007439 A CN 109007439A CN 201810826549 A CN201810826549 A CN 201810826549A CN 109007439 A CN109007439 A CN 109007439A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention is provided containing callicarpa nudiflora prawn feed additive and its preparation method and application, it is made of Chinese herbal medicine, probiotics, acidulant, enzyme preparation, oligosaccharide and maize cob meal, prawn of the invention composite feed additive has many advantages, such as pollution-free, noresidue, has no toxic side effect, safety and environmental protection, immunity of prawn and premunition can be improved, improve the survival rate of prawn while having effects that food calling is somatotrophic.
Description
Technical field
The invention belongs to animal feed additive fields, and in particular to containing callicarpa nudiflora prawn feed additive and its system
Preparation Method and application.
Background technique
In recent years, since feeding management is improper and breeding environment deteriorates, breaking out for disease of prawn is just supported as limitation prawn
Grow the principal element of industry development.Various regions prawn falls ill, and phenomenon is fairly common, and prawn disease type is more and more, some prawn diseases go out shrimp ponds
The serious situation now having no harvest.At present in prawn culturing mode, pursuing high density, high yield and high benefit is common feature, is carried out
The prevention work of disease of prawn is to improve one of prawn culturing yield and the important measures of economic benefit.
Prevent and treat disease of prawn conventional method be using antibiotic, however antibiotic for a long time be widely used and can lead
Cause the serious pollution of breeding water body, medicament residue, endurance strain generation the problems such as, reduce the quality and nutriture value of prawn
Value, finally jeopardizes the health of mankind itself.Chinese herbal medicine have green natural, resourceful, performance is various, toxic side effect is small, low
The advantages that residual, the prospect with substitute antibiotics or chemicals.Chinese herbal medicine can not only effectively prevent disease, and can improve
Prawn production performance and efficiency of feed utilization, increasingly by the favor of poultry feeders in aquaculture.
The problem that therapeutic effect based on the induction of above-mentioned feed is not good enough, developing the new prawn feed additive of one kind becomes urgent
It is essential and asks.
Summary of the invention
The primary purpose of the present invention is that providing containing callicarpa nudiflora prawn feed additive.
A further object of the present invention is to provide containing callicarpa nudiflora prawn feed addictive preparation method.
Another object of the present invention is to provide containing callicarpa nudiflora prawn feed additive application.
The technical solution used in the present invention is:
It is callicarpa nudiflora to prepare the application in animal feed additive.
Further, the animal is prawn.
A kind of prawn feed additive, the feed addictive contain callicarpa nudiflora.
Further, which contains 26~100 parts of Chinese herbal medicines, 5~20 parts of probiotics, 5~20 parts of acidulants, 5~10
Part enzyme preparation, 5~10 portions of oligosaccharide and 20~40 parts of corncobs.
Further, the Chinese herbal medicine is callicarpa nudiflora by 10~20 parts, 10~20 portions of erythrina indicas, 1~10 part of cactus, 1
~10 parts of chenopodium ambrosiodies, 1~10 portion of Radix Glycyrrhizae, 1~10 part of Eclipta prostrata, 1~10 portion of Verbena officinalis and 1~10 portion of garlic are made.
Further, the probiotics is bacillus subtilis, Miyarisan, Bifidobacterium, at least one in photosynthetic bacteria
Kind.
Further, the acidulant is at least one of fumaric acid, citric acid and malic acid.
Further, the enzyme preparation is at least one of amylase, protease, lipase and zytase.
Further, the oligosaccharide is at least one of zymosan, chitosan and mannosan.
A kind of preparation method of prawn feed additive, it is shown that steps are as follows:
(1) callicarpa nudiflora dry powder, erythrina indica dry powder, cactus dry powder, chenopodium ambrosiodies dry powder, sweet is weighed according to formula described above
Careless dry powder, Eclipta prostrata dry powder, Verbena officinalis dry powder and garlic dry powder, mixing for standby use；
(2) probiotics, acidulant, maize cob meal, enzyme preparation and oligosaccharide are added to (1) to be uniformly mixed, crossing mesh is
Obtain animal feed additive.
Further, mesh described in step (2) is 50~100 meshes.
It is callicarpa nudiflora to improve prawn serum superoxide dismutases, serum lysozyme or/and serum phenol oxidase in preparation
The feed of content or the application in feed addictive.
The callicarpa nudiflora application in the feed or feed addictive that preparation improves immunity.
The callicarpa nudiflora application in the feed or feed addictive that preparation reduces feed coefficient.
The callicarpa nudiflora application in the feed or feed addictive that preparation promotes prawn survival rate.
The callicarpa nudiflora application induced in preparation in the feed or feed addictive that promote prawn growth.
The beneficial effects of the present invention are:
The present invention by five kinds of Chinese herbal medicine, probiotics, acidulant, enzyme preparation and oligosaccharide functional additive combination and compatibilities,
Improve the food ration of prawn.The prawn heart, liver, kidney equilibrium between yin and yang are fundamentally adjusted, the recovery of immunity of organism balance is facilitated.
Chinese herbal medicine can effectively help disease shrimp body eliminating pathogen to support vital qi and consolidate in side, repair to the body damaged, regulate certainly
The equilibrium between yin and yang of body improves prawn constitution, improves the survival rate of prawn, improve the immune level of prawn while having food calling
The effect of promoting prawn growth.
Specific embodiment
It is callicarpa nudiflora to prepare the application in animal feed additive.
Preferably, the animal is prawn.
A kind of prawn feed additive, the feed addictive contain callicarpa nudiflora.
Preferably, which contains 26~100 parts of Chinese herbal medicines, 5~20 parts of probiotics, 5~20 parts of acidulants, 5~10 parts
Enzyme preparation, 5~10 portions of oligosaccharide and 20~40 parts of corncobs.
Preferably, the Chinese herbal medicine is callicarpa nudiflora by 10~20 parts, 10~20 portions of erythrina indicas, 1~10 portion of cactus, 1~
10 parts of chenopodium ambrosiodies, 1~10 portion of Radix Glycyrrhizae, 1~10 part of Eclipta prostrata, 1~10 portion of Verbena officinalis and 1~10 portion of garlic are made.
Preferably, the probiotics is at least one of bacillus subtilis, Miyarisan, Bifidobacterium, photosynthetic bacteria.
Preferably, the acidulant is at least one of fumaric acid, citric acid and malic acid.
Preferably, the enzyme preparation is at least one of amylase, protease, lipase and zytase.
Preferably, the oligosaccharide is at least one of zymosan, chitosan and mannosan.
A kind of preparation method of prawn feed additive, it is shown that steps are as follows:
(1) callicarpa nudiflora dry powder, erythrina indica dry powder, cactus dry powder, chenopodium ambrosiodies dry powder, sweet is weighed according to formula described above
Careless dry powder, Eclipta prostrata dry powder, Verbena officinalis dry powder and garlic dry powder, mixing for standby use；
(2) probiotics, acidulant, maize cob meal, enzyme preparation and oligosaccharide are added to (1) to be uniformly mixed, crossing mesh is
Obtain animal feed additive.
Preferably, mesh described in step (2) is 50~100 meshes.
It is callicarpa nudiflora to improve prawn serum superoxide dismutases, serum lysozyme or/and serum phenol oxidase in preparation
The feed of content or the application in feed addictive.
The callicarpa nudiflora application in the feed or feed addictive that preparation improves immunity.
The callicarpa nudiflora application in the feed or feed addictive that preparation reduces feed coefficient.
The callicarpa nudiflora application in the feed or feed addictive that preparation promotes prawn survival rate.
The callicarpa nudiflora application induced in preparation in the feed or feed addictive that promote prawn growth.
The present invention is further illustrated combined with specific embodiments below.
A kind of animal feed additive of embodiment 1 and preparation method thereof
(1) by middle medicinal herbs: callicarpa nudiflora, erythrina indica, cactus, chenopodium ambrosiodies, Radix Glycyrrhizae, Eclipta prostrata, Verbena officinalis and garlic are cleaned simultaneously
Chopping is crushed, and obtain powdered Chinese herbal medicine with the screening of 80 mesh after drying machine drying with pulverizer；
(2) it is dry that 20 parts of powdered callicarpa nudiflora dry powder, 20 parts of erythrina indica dry powder, 10 parts of cactus dry powder, chenopodium ambrosiodies are weighed
10 parts of powder, 10 parts of Radix Glycyrrhizae dry powder, 10 parts of Eclipta prostrata dry powder, 10 parts of Verbena officinalis dry powder and 10 parts of garlic dry powder；
(3) 5 parts of Miyarisans, 5 parts of photosynthetic bacterias, 5 parts of fumaric acid, 5 parts of malic acid, 20 parts of maize cob meals, 2.5 parts of albumen are weighed
Enzyme, 2.5 parts of lipase and 5 parts of mannosans；
(4) (2) and (3) are uniformly mixed in mixing machine, and crosses 80 meshes, obtain feed addictive.
A kind of animal feed additive of embodiment 2 and preparation method thereof
(1) by middle medicinal herbs: callicarpa nudiflora, erythrina indica, cactus, chenopodium ambrosiodies, Radix Glycyrrhizae, Eclipta prostrata, Verbena officinalis and garlic are cleaned simultaneously
Chopping is crushed, and obtain powdered Chinese herbal medicine with the screening of 80 mesh after drying machine drying with pulverizer；
(2) 15 parts of powdered callicarpa nudiflora dry powder, 15 parts of erythrina indica dry powder, 5 parts of cactus dry powder, chenopodium ambrosiodies dry powder are weighed
5 parts, 5 parts of Radix Glycyrrhizae dry powder, 5 parts of Eclipta prostrata dry powder, 5 parts of Verbena officinalis dry powder and 5 parts of garlic dry powder；
(3) 5 parts of Miyarisans, 5 parts of photosynthetic bacterias, 5 parts of fumaric acid, 5 parts of malic acid, 20 parts of maize cob meals, 2.5 parts of albumen are weighed
Enzyme, 2.5 parts of lipase and 5 parts of zymosans；
(4) (2) and (3) are uniformly mixed in mixing machine, and crosses 80 meshes, obtain feed addictive.
A kind of animal feed additive of embodiment 3 and preparation method thereof
(1) by middle medicinal herbs: callicarpa nudiflora, erythrina indica, cactus, chenopodium ambrosiodies, Radix Glycyrrhizae, Eclipta prostrata, Verbena officinalis and garlic are cleaned simultaneously
Chopping is crushed, and obtain powdered Chinese herbal medicine with the screening of 80 mesh after drying machine drying with pulverizer；
(2) 10 parts of powdered callicarpa nudiflora dry powder, 10 parts of erythrina indica dry powder, 1 part of cactus dry powder, chenopodium ambrosiodies dry powder are weighed
1 part, 1 part of Radix Glycyrrhizae dry powder, 1 part of Eclipta prostrata dry powder, 1 part of Verbena officinalis dry powder and 1 part of garlic dry powder；
(3) 5 parts of bacillus subtilises, 5 parts of Bifidobacteriums, 5 parts of citric acids, 5 parts of malic acid, 40 parts of maize cob meals, 1 are weighed
Part zytase, 1 part of amylase and 8 parts of chitosans；
(4) (2) and (3) are uniformly mixed in mixing machine, and crosses 80 meshes, obtain feed addictive.
A kind of animal feed additive of embodiment 4 and preparation method thereof
(1) by middle medicinal herbs: callicarpa nudiflora, erythrina indica, cactus, chenopodium ambrosiodies, Radix Glycyrrhizae, Eclipta prostrata, Verbena officinalis and garlic are cleaned simultaneously
Chopping is crushed, and obtain powdered Chinese herbal medicine with the screening of 80 mesh after drying machine drying with pulverizer；
(2) it is dry that 20 parts of powdered callicarpa nudiflora dry powder, 20 parts of erythrina indica dry powder, 10 parts of cactus dry powder, chenopodium ambrosiodies are weighed
10 parts of powder, 10 parts of Radix Glycyrrhizae dry powder, 10 parts of Eclipta prostrata dry powder, 10 parts of Verbena officinalis dry powder and 10 parts of garlic dry powder；
(3) 10 parts of bacillus subtilises, 10 parts of Bifidobacteriums, 10 parts of citric acids, 10 parts of malic acid, 30 parts of corncobs are weighed
Powder, 10 parts of zytases, 5 parts of amylase and 10 parts of zymosans；
(4) (2) and (3) are uniformly mixed in mixing machine, and crosses 80 meshes, obtain feed addictive.
The effect of the raising prawn growth performance of embodiment 5
750 tail of healthy Penaeus Vannmei that original body mass is (2.00 ± 0.05) g is randomly selected, is randomly divided into 5 groups, every group
If 3 repetitions, each 50 tail of repetition is temporarily supported in 15 80cm × 40cm × 60cm transparent glass cylinders, the very useful gauze cover of cylinder
Firmly, prevention and treatment prawn escape, while convenient for feeding, removing contamination and observe.Test group feeds addition Examples 1 to 4 feed addition respectively
The bait (1g/kg bait) of agent addition, control group bait are conventional basic daily ration, and other rearing conditions are consistent.
Preliminary trial period 7 days, experimental period 35 days.It feeds 4 times daily, subject to daily ration, feeding quantity is not remaining in two hours, record is daily
Daily ration, feeding quantity.Water temperature is maintained at 25 ± 2 DEG C during test, continuous charge in 24 hours.
For NSC 334200 transaminase (ALT), the measurement of aspartic transaminase (AST) and alkaline phosphatase (ALP).
The measurement of ALT, AST, ALP are tested using the kit that Bioengineering Research Institute's production is built up in Nanjing.Concrete operation method
It is carried out according to kit specification.
After experimental period, measure Penaeus Vannmei growth indexes, including weight, rate of body weight gain, feed coefficient and at
Motility rate.Meanwhile 5 tail grass carps are taken at random from each glass jar, blood is taken in tail vein.After the 4 DEG C of placement 2h of whole blood extracted, in
3500r/min, 4 DEG C of centrifugation 30min in centrifuge, taking supernatant is serum, -20 DEG C of preservations；Bioengineering is built up using Nanjing to grind
Study carefully institute total number born (T-SOD) testing cassete, lysozyme (LZM) testing cassete, the detection of polyphenol oxidase (PPO) testing cassete
Prawn salivary lysozyme: serum superoxide dismutases SOD (u/mg), serum lysozyme UI (U/ml) and serum phenol
The numerical value of oxidizing ferment PO (U/ml), concrete operation method are carried out according to kit specification.
Test result such as Tables 1 and 2.
Influence of the table 1 containing callicarpa nudiflora feed for prawn additive to Penaeus Vannmei
Influence of prawn dedicated Chinese herbal feed additive of the table 2 containing callicarpa nudiflora end to Penaeus Vannmei immunity of organisms
In summary result it is found that the present invention can be improved the serum superoxide dismutases of prawn, serum lysozyme and
The level of blood phenol oxidase can improve the immunity and premunition of prawn.In addition the present invention improves the survival rate of prawn, improves
Efficiency of feed utilization reduces feed coefficient, can promote the growth of prawn.
The effect that embodiment 6 is applied in prawn culturing
In Jiangmen District of Guangdong Province, the feed containing 2 feed addictive of embodiment is used in the shrimp pool of 5 peasant households, with throwing
It feeds the feed containing callicarpa nudiflora prawn feed additive to compare, the prawn for feeding the latter is energetic, body colour is good, prawn disease incidence drop
Low 35~40%, the culture-cycle shortens 5~7 days, and culture efficiency increases.
In summary the result shows that, feed addictive of the invention can effectively improve immunity of prawn and premunition, reduce
Disease incidence, food calling growth promotion increase culture efficiency.
The above is the preferable embodiment of the present invention, but embodiments of the present invention are not by the limit of above-described embodiment
System, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. callicarpa nudiflora preparing the application in animal feed additive.
2. application according to claim 1, which is characterized in that the animal is prawn.
3. a kind of prawn feed additive, which is characterized in that the feed addictive contains callicarpa nudiflora.
4. prawn feed additive according to claim 3, which is characterized in that the feed contain 26~100 parts of Chinese herbal medicines,
5~20 parts of probiotics, 5~20 parts of acidulants, 5~10 parts of enzyme preparations, 5~10 portions of oligosaccharide and 20~40 parts of corncobs.
5. prawn feed additive according to claim 4, which is characterized in that the Chinese herbal medicine is by 10~20 parts of nakedflowers
Callicarpa bodinieri Levl., 10~20 portions of erythrina indicas, 1~10 portion of cactus, 1~10 part of chenopodium ambrosiodies, 1~10 portion of Radix Glycyrrhizae, 1~10 part of Eclipta prostrata, 1~10 part
Verbena officinalis and 1~10 portion of garlic are made.
6. prawn feed additive according to claim 4, which is characterized in that the probiotics is bacillus subtilis
At least one of bacterium, Miyarisan, Bifidobacterium, photosynthetic bacteria.
7. a kind of preparation method of prawn feed additive, which is characterized in that shown in steps are as follows:
(1) callicarpa nudiflora dry powder, erythrina indica dry powder, cactus dry powder, chenopodium ambrosiodies is weighed according to formula described in claim 3~5 to do
Powder, Radix Glycyrrhizae dry powder, Eclipta prostrata dry powder, Verbena officinalis dry powder and garlic dry powder, mixing for standby use；
(2) probiotics, acidulant, maize cob meal, enzyme preparation and oligosaccharide are added to (1) to be uniformly mixed, cross mesh up to dynamic
Object feed addictive.
8. the callicarpa nudiflora superoxide dismutase in preparation raising immunity of prawn, raising prawn serum, raising serum are molten
The feed or the application in feed addictive of bacterium enzyme or/and serum phenol oxidase content.
9. the callicarpa nudiflora application in the feed or feed addictive that preparation reduces prawn feed coefficient.
10. the callicarpa nudiflora application induced in preparation in the feed or feed addictive that promote prawn growth.
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| CN201810826549.4A CN109007439B (en) | 2018-07-25 | 2018-07-25 | Prawn feed additive containing callicarpa nudiflora and preparation method and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110810667A (en) * | 2019-11-25 | 2020-02-21 | 江苏昌浩水产科技有限公司 | Mixed fish feed additive |
| CN114558128A (en) * | 2022-03-17 | 2022-05-31 | 湖南师范大学 | Callicarpa nudiflora serving as fish vaccine adjuvant and application thereof |
| CN115997707A (en) * | 2023-03-06 | 2023-04-25 | 湖南师范大学 | Catfish offspring seed cultivation solution, catfish offspring seed cultivation method and application |
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| CN102318771A (en) * | 2011-09-20 | 2012-01-18 | 广东粤海饲料集团有限公司 | Chinese medicinal herb disease-resistant additive for prawn feed |
| CN103652452A (en) * | 2013-12-06 | 2014-03-26 | 中国科学院海洋研究所 | Novel biological feed for prawn and application thereof |
| CN105078803A (en) * | 2014-05-16 | 2015-11-25 | 苏州润新生物科技有限公司 | Application of callicarpa plant extract |
| CN104012801A (en) * | 2014-06-11 | 2014-09-03 | 湖南中医药大学 | Poultry feed containing callicarpa nudiflora and preparing method of poultry feed |
| CN104171374A (en) * | 2014-07-10 | 2014-12-03 | 南宁荣港生物科技有限公司 | Preparation method of traditional Chinese medicinal feed additive for prawns |
| CN105746898A (en) * | 2016-03-09 | 2016-07-13 | 广州格雷特生物科技有限公司 | Chinese herbal medicine feed additive with Folium Callicarpae Nudiflorae and preparation method and application thereof |
| CN107149053A (en) * | 2017-03-22 | 2017-09-12 | 广东粤佳饲料有限公司 | A kind of compound additive premix for promoting Penaeus monodon growth and preparation method thereof and application method |
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| CN110810667A (en) * | 2019-11-25 | 2020-02-21 | 江苏昌浩水产科技有限公司 | Mixed fish feed additive |
| CN114558128A (en) * | 2022-03-17 | 2022-05-31 | 湖南师范大学 | Callicarpa nudiflora serving as fish vaccine adjuvant and application thereof |
| CN114558128B (en) * | 2022-03-17 | 2024-01-30 | 湖南师范大学 | Callicarpa nudiflora as fish vaccine adjuvant and application thereof |
| CN115997707A (en) * | 2023-03-06 | 2023-04-25 | 湖南师范大学 | Catfish offspring seed cultivation solution, catfish offspring seed cultivation method and application |
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