CN108774631B - A kind of preparation method of rock kinases - Google Patents
A kind of preparation method of rock kinases Download PDFInfo
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- CN108774631B CN108774631B CN201811112255.1A CN201811112255A CN108774631B CN 108774631 B CN108774631 B CN 108774631B CN 201811112255 A CN201811112255 A CN 201811112255A CN 108774631 B CN108774631 B CN 108774631B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
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Abstract
The invention discloses a kind of preparation method of rock kinases, the mass ratio of the constitutive material for including and each raw material is natural rock micro mist: chelating agent: complex enzyme: pure water=1:0.05 ~ 0.1:0.1 ~ 0.5:1.5 ~ 3；The natural rock micro mist is made of following raw material according to mass percentage: Tourmaline stone micro mist 15% ~ 30%, germanite micro mist 15% ~ 25%, medical stone micro mist 15% ~ 35%, pelelith micro mist 20% ~ 35%, selenium ore micro mist 5% ~ 15%；Preparation step includes being prepared into natural rock micro mist under the conditions of high temperature and pressure Strong oxdiative after rock crushes, and pure water is added and chelating agent carries out chelatropic reaction, complex enzyme is added and is chelated, rock kinases is prepared.The present invention is by being prepared complex enzyme and trace element chelated the microelement of the high concentration of activity and the compound formulation of enzyme, improving the functioning efficiency of enzyme.
Description
Technical field
The present invention relates to enzyme preparation technical fields, and in particular to a kind of preparation method of rock kinases.
Background technique
Enzyme preparation is widely used in food processing, feed, weaving, chemical industry, sewage treatment and agricultural etc. at present.
Industrial expansion causes very big pollution, and wherein cost of sewage disposal is very high, is extremely difficult to national standard, the reason is that being difficult water
Molecule reduction, even if using corresponding biological method such as processing with enzyme preparation sewage, it is dirty due to pollutant decomposition efficiency is low etc.
The effect of water process is poor.And agriculturally since soil long-term cultivation is unable to get recuperation, Trace Elements in Soil is serious
Missing, and single chemical fertilizer is used for a long time, balanced micro of the fruits and vegetables serious loss that the chemical fertilizer rich in elements such as N P and Ks is grown
Element, maintaining this chemical fertilizer to promote the precondition of growth is to inhibit insect pest using a large amount of pesticides, causes vegetables and fruits pesticide residue super
Mark, not can effectively reduce the product and method of pesticide residue, using in relevant enzyme preparation degrading pesticide in the prior art
Noxious material when, degradation speed is slow, and degradation effect is poor.
Microelement is the indispensable part of institute in organism metabolic activity.For medical angle, microelement is
The supporting frame in body energy source, the missing of Trace Elements in Human Body will lead to iron-deficient heart disease, hypoferric anemia, leucoderma
Etc. diseases；Microelement is widely applied in medical product at present, such as 21 Jin Weita, multivitamin Centrum
Deng.But current research is usually limited only to how preferably to prepare microelement, temporarily ties independent of between microelement and enzyme
Close the research of application.
1609205 A of patent CN discloses a kind of preparation method of degradation complex enzyme, selects lignin peroxidase, phytic acid
Enzyme, phenol oxidase, formamidase, cyaniding hydrolase, formic acid glue hydrogen enzyme, lysozyme, tween solution, be mixed by sorting,
Dry, packaging and other steps are made, and realize degerming, decontamination, remove heavy metal pollution removing, degrading pesticide antibiotic hormone residues etc. and making
With, but the type and content of enzyme are higher, and at high cost, enzymatic activity is lower, and reaction efficiency is lower.
Summary of the invention
In order to solve existing technical problem, the present invention provides a kind of preparation method of rock kinases, by by complex enzyme
With it is trace element chelated, the microelement of the high concentration of activity and the compound formulation of enzyme is prepared, improves the functioning efficiency of enzyme.
The technical scheme is that a kind of preparation method of rock kinases, the quality of the constitutive material for including and each raw material
Than for natural rock micro mist: chelating agent: complex enzyme: pure water=1: 0.05 ~ 0.1: 0.1 ~ 0.5: 1.5 ~ 3；It is described natural
Rock micro mist is made of following raw material according to mass percentage: Tourmaline stone micro mist 15% ~ 30%, germanite micro mist 15% ~ 25%, wheat
Meal stone micro mist 15% ~ 35%, pelelith micro mist 20% ~ 35%, selenium ore micro mist 5% ~ 15%；
Preparation step is as follows:
(1) Tourmaline stone, germanite, medical stone, pelelith, selenium ore are ground into 1000 mesh with down toward nanoscale respectively,
It is prepared into the micro mist of various rocks respectively, according to the constitutive material and mass percent of natural rock micro mist, weighs corresponding rock
Stone micro mist is put into pyroreaction kettle, temperature is 120 DEG C, pressure is 2 × 105It is anti-under the conditions of the high temperature and pressure Strong oxdiative of Pa
5h is answered, natural rock micro mist is formed；
(2) by the natural rock micro mist cooled to room temperature in step (1), pure water, chelating agent is then added, in temperature
Under conditions of degree is 28 DEG C, chelatropic reaction is carried out for 24 hours；
(3) in step (2) after chelatropic reaction, then cooled to room temperature is added multiple into pyroreaction kettle
Synthase stirs evenly, and carries out chelatropic reaction for 24 hours under normal temperature conditions, rock kinases is prepared.
Preferably, the mass ratio of the rock kinases includes constitutive material and each raw material is natural rock micro mist: chelating agent:
Complex enzyme: pure water=1:0.08:0.3:2.
Preferably, the natural rock micro mist is made of following raw material according to mass percentage: Tourmaline stone micro mist
20%, germanite micro mist 20%, medical stone micro mist 25%, pelelith micro mist 25%, selenium ore micro mist 10%.
Preferably, the complex enzyme is one of animal protease or plant complex enzyme.
Preferably, the complex enzyme is organic phosphorus degrading enzyme, carbamatic hydrolase and pyrethroid ester hydrolase
Mixture.
In a kind of preparation method of rock kinases of the invention, Tourmaline stone contains whole macroelements of animal needs, such as
K, Na, Ca, Mg etc., the microelements such as Cu, Mo and rare earth element, share 50 various trace elements.A large amount of germanium is in germanite to divide
Bulk state is present in the silicate mine, sulfide mine and various types of coals of various metals.Medical stone contains needed for animal
Whole macroelements, such as the microelements such as K, Na, Ca, Mg and Cu, Mo and rare earth element, as many as about 58 kinds.Contain in pelelith
There are tens kinds of minerals such as Na, Mg, Al, Si, Ca, Ti, Mn, Fe, Ni, Co, Mo and microelement.Rich in Se member in selenium ore
Element.By being prepared into natural rock micro mist, and water mixing is added in microelement rich in natural rock, with chelating agent,
Complex enzyme chelating after, microelement can remarkably promote enzyme cell activity, fast and effeciently microelement-supplementing, adjust body
It is balanced.Plant complex enzyme can be used for plant in the high catalytic efficiency of rock kinases, and for animal, animal protein can be used
Enzyme.Rock kinases prepared by the present invention can be widely applied to medicine, health care, beauty, improvement soil, environmental protection, sewage are controlled
Reason, plantation cultivation etc..In terms of sewage treatment, Equilibrium of Trace Elements water quality in rock kinases, and promote the growth of enzyme, fastly
Speed decomposes the pollutant in water, to effectively administer sewage.In terms of planting cultivation, while using rock kinases, it can continue
Using pesticide, rock kinases mainly passes through enzyme and is converted into other innocuous substances in conjunction with chemistry of pesticide molecule, thus agriculture of effectively degrading
Medicine residual.By taking organic phosphorus degrading enzyme as an example, organic phosphorus degrading enzyme by cutting noxious material in P-O key, P-F key, P-S key,
Macromolecular moment toxic, not soluble in water is degraded into small molecule that is nontoxic, being dissolved in water and will not cause two to environment by P-CN key
Secondary pollution is a kind of nontoxic, efficient, environmentally friendly biological high-tech product.Organic phosphorus degrading enzyme is added in some noxious materials,
Its degradation speed can be made to improve 1000 to 2450 times；And rock kinases is the promotor of organic phosphorus degrading enzyme, can quickly be supplemented micro-
Secondary element promotes organic phosphorus degrading enzyme and chemistry of pesticide point by the neutralization of acid-base neutralization reaction, microelement and microelement
Son combines and is converted into other innocuous substances, promotes the growths of crops, effective degrading pesticide residues.
Having the beneficial effect that by adopting the above technical scheme:
(1) of the invention by complex enzyme and trace element chelated, fast and effeciently microelement-supplementing, improves rock kinases
Activity improves the functioning efficiency of rock kinases.
(2) animal protease is used for animal in the present invention, uses plant complex enzyme, the rock being prepared for plant
Kinases is widely used in the fields such as medicine, environmental improvement, plantation cultivation；It, can when especially rock kinases is used to plant cultivation field
It effectively degrades remaining pesticide.
(3) preparation method is simple, easily operated, and production cost is low, can be widely applied to produce.
Specific embodiment
The present invention will be further described in detail below with reference to specific embodiments.
Embodiment 1
A kind of preparation method of rock kinases, the quality of the constitutive material for including and each raw material are as follows: natural rock micro mist 100g,
Chelating agent 8g, complex enzyme 30g, pure water 200g；The natural rock micro mist is made of the quality of following raw material and raw material: Tuo Ma
Beautiful jade stone micro mist 20g, germanite micro mist 20g, medical stone micro mist 25g, pelelith micro mist 25g, selenium ore micro mist 10g；
Preparation step is as follows:
(1) Tourmaline stone, germanite, medical stone, pelelith, selenium ore are ground into 1000 mesh with down toward nanoscale respectively,
It is prepared into the micro mist of various rocks respectively, according to the constitutive material and quality of natural rock micro mist, weighs corresponding rock micro mist,
It puts into pyroreaction kettle, temperature is 120 DEG C, pressure is 2 × 1055h, shape are reacted under the conditions of the high temperature and pressure Strong oxdiative of Pa
At natural rock micro mist；
(2) by the natural rock micro mist cooled to room temperature in step (1), 200g pure water, 8g chelating is then added
Agent carries out chelatropic reaction for 24 hours under conditions of temperature is 28 DEG C；
(3) in step (2) after chelatropic reaction, then 30g is added in cooled to room temperature into pyroreaction kettle
Complex enzyme stirs evenly, and carries out chelatropic reaction for 24 hours under normal temperature conditions, rock kinases is prepared.
The complex enzyme is the mixture of organic phosphorus degrading enzyme, carbamatic hydrolase and pyrethroid ester hydrolase.
By the rock kinases of the present embodiment be applied to plantation cultivation field, such as tealeaves, radish, eggplant, leek, shallot kind
It plants, normal use pesticide in planting process, and uses rock kinases simultaneously, quality inspection, quality inspection are carried out to the product after maturation
It tests commission Ministry of Agriculture's food quality supervision verification test center (Shanghai) to carry out, inspection result shows that all products do not have
Pesticide residue, by taking tealeaves and eggplant as an example, specific inspection result is as follows:
(1) shown in tea quality inspection result chart 1:
1 tealeaves inspection result report of table
According to table 1, Tea planting is carried out using rock kinases, obtained tea products do not have pesticide residue, illustrate rock
Kinases can effectively degrade pesticide residue used in Tea planting process.
(2) shown in eggplant quality test results chart 2:
2 eggplant inspection result report of table
According to table 2, eggplant plantation is carried out using rock kinases, obtained product does not have pesticide residue, illustrates rock kinases
Can effectively be degraded pesticide used in eggplant planting process.
In summary content and inspection result it is found that plantation breeding process in, using while different pesticides use rock
Kinases, can quick microelement-supplementing, promote crop growth, and remaining pesticide of degrading.
Claims (5)
1. a kind of preparation method of rock kinases, it is characterised in that: the quality of constitutive material and each raw material that the rock kinases includes
Than for natural rock micro mist: chelating agent: complex enzyme: pure water=1:0.05~0.1:0.1~0.5:1.5~3；The natural rock
Stone micro mist is made of following raw material according to mass percentage: Tourmaline stone micro mist 15%~30%, germanite micro mist 15%~
25%, medical stone micro mist 15%~35%, pelelith micro mist 20%~35%, selenium ore micro mist 5%~15%；
Preparation step is as follows:
(1) Tourmaline stone, germanite, medical stone, pelelith, selenium ore are ground into 1000 mesh down toward nanoscale, difference respectively
It is prepared into the micro mist of various rocks, according to the constitutive material and mass percent of natural rock micro mist, it is micro- to weigh corresponding rock
Powder is put into pyroreaction kettle, temperature is 120 DEG C, pressure is 2 × 1055h is reacted under the conditions of the high temperature and pressure Strong oxdiative of Pa,
Form natural rock micro mist；
(2) by the natural rock micro mist cooled to room temperature in step (1), pure water, chelating agent is then added, is in temperature
Under conditions of 28 DEG C, chelatropic reaction is carried out for 24 hours；
(3) in step (2) after chelatropic reaction, then complex enzyme is added in cooled to room temperature into pyroreaction kettle,
It stirs evenly, carries out chelatropic reaction for 24 hours under normal temperature conditions, rock kinases is prepared.
2. a kind of preparation method of rock kinases as described in claim 1, it is characterised in that: the composition that the rock kinases includes is former
The mass ratio of material and each raw material is natural rock micro mist: chelating agent: complex enzyme: pure water=1:0.08:0.3:2.
3. a kind of preparation method of rock kinases as described in claim 1, it is characterised in that: the natural rock micro mist is according to matter
Amount percentage meter is made of following raw material: Tourmaline stone micro mist 20%, germanite micro mist 20%, medical stone micro mist 25%, pelelith
Micro mist 25%, selenium ore micro mist 10%.
4. a kind of preparation method of rock kinases as described in claim 1, it is characterised in that: the complex enzyme is animal protease
Or one of plant complex enzyme.
5. a kind of preparation method of rock kinases as described in claim 1 or 4, it is characterised in that: the complex enzyme is organic phosphorus
The mixture of degrading enzyme, carbamatic hydrolase and pyrethroid ester hydrolase.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104829272A (en) * | 2009-12-22 | 2015-08-12 | 拉尔科营养品有限公司 | Organically chelated mineral compositions and methods thereof |
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| CN101156659A (en) * | 2007-11-12 | 2008-04-09 | 河南三农生态产业有限公司 | Organic swine core premix |
| CN105053726A (en) * | 2015-09-11 | 2015-11-18 | 安徽科技学院 | Fodder capable of stimulating appetite of pheasant for table purpose |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104829272A (en) * | 2009-12-22 | 2015-08-12 | 拉尔科营养品有限公司 | Organically chelated mineral compositions and methods thereof |
Non-Patent Citations (2)
| Title |
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| Effects of soil and foliar application of soluble silicon on mineral nutrifion，gas exchange，and growth of potato plants;PILON CRISTIANE等;《Crop Science》;20131231;第53卷(第4期);全文 |
| 喷施不同纳米硅肥对红苋菜增产效果研究;裴福云等;《安徽农业科学》;20151231;第43卷(第2期);全文 |
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