CN108293762A - A kind of cultural method improving non-irrigated lotus root quality - Google Patents
A kind of cultural method improving non-irrigated lotus root quality Download PDFInfo
- Publication number
- CN108293762A CN108293762A CN201710766061.2A CN201710766061A CN108293762A CN 108293762 A CN108293762 A CN 108293762A CN 201710766061 A CN201710766061 A CN 201710766061A CN 108293762 A CN108293762 A CN 108293762A
- Authority
- CN
- China
- Prior art keywords
- lotus root
- filtrate
- selenium
- added
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
- C05F1/005—Fertilisers made from animal corpses, or parts thereof from meat-wastes or from other wastes of animal origin, e.g. skins, hair, hoofs, feathers, blood
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Chemical & Material Sciences (AREA)
- Soil Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Organic Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Fertilizers (AREA)
Abstract
The present invention relates to agricultural technology fields, and in particular to a kind of cultural method improving non-irrigated lotus root quality, concrete operations are as follows：S1, plantation；S2, field management：A applies top dressing：2 top dressings are carried out in the growth period of non-irrigated lotus root, are carried out in first time when there are 35 vertical leaves, dose is 800 1000kg per acre；Second of top dressing is carried out when tying lotus root, and dose is 800 1000kg per acre；B weedings：It is carried out when there are weeds within 20 30 days after kind of lotus root is planted, the weeds pulled up is buried in the earth；C is retained：It is retained using irrigation system, keeps plantation field humidity 80% 90%；S2, harvesting：Start to dig lotus root 3 April, digging lotus root first half of the month is drained.The application implantation methods are simple and practicable, and the ratio of the various elements content in non-irrigated lotus root body can be improved, so that non-irrigated lotus root quality-improving, promotes the economic value of non-irrigated lotus root, achieve the purpose that increasing peasant income.
Description
【Technical field】
The present invention relates to agricultural technology fields, and in particular to a kind of cultural method improving non-irrigated lotus root quality.
【Background technology】
Banana dasheen is also known as edible canna, any of several broadleaf plants lotus root, achira, non-irrigated taro etc., is a kind of heat for China being passed to by Indonesia the thirties
Band plant, has large area plantation, due to the root of banana dasheen in the Guizhou Province in China, Sichuan Province, Yunnan Province, Guangxi Zhuang Autonomous Region
Stem contains the nutritional ingredient of abundant starch and needed by human body, and has the function of that and banana dasheen contains with similar in potato starch
Have with the comparable protein of potato quantity, amino acid classes are complete, it is necessary to which total amino acid content proportion is larger, E/T ratios
(i.e. necessary amino acid content nitrogen content more total than protein) and egg, the E/T ratios of cow's milk are suitable.Higher than cereal, beans and its
The protein E/T ratios of his potato.As people are continuously increased healthy food demand, the demands such as dry lotus root starch, dry lotus root vermicelli
Will gradually it increase.
Guangxi hydrothermal resources are abundant, and loosing soil, permeability is strong, are suitable for non-irrigated lotus root growth.《The Guangxi 2011-2015 kind
Zhi Ye superior products Regional Distribution plans》It points out, early lotus root is the two of the Ways of Special Agricultural Products that Nanning City, Hechi City are given priority to,
2015 annual planting areas were doubled than 2005, and stem tuber per unit area yield improves 30%.Current drought lotus root cultivation management is extensive, cultivation technique
Fall behind relatively, based on being grown naturally in field, Around the house, the natural environment of growth is poor, low output.Therefore one is found
The implantation methods that kind can improve non-irrigated lotus root quality can be only achieved so as to be in great demand to the purpose of increasing peasant income.
【Invention content】
The goal of the invention of the present invention is：For above-mentioned problem, the present invention provides a kind of cultivation improving non-irrigated lotus root quality
Method, this method is simple and practicable, and the ratio of the various elements content in non-irrigated lotus root body can be improved, so that non-irrigated lotus root quality-improving,
The economic value for promoting non-irrigated lotus root, achievees the purpose that increasing peasant income.
To achieve the goals above, the technical solution adopted by the present invention is as follows：
A kind of cultural method improving non-irrigated lotus root quality, concrete operations are as follows：
S1, plantation：The rhizoma nelumbinis for selecting a section long and with 3-5 bud is sowed as seed, is carried out using thimerosal
It using after distilled water flushing after impregnating 3-5 minutes, can be sowed after dipping root-inducing powder, sowing is since at sowing field side 1.5m
It is transplanted by going, arrangement will be interacted by planting the direction of lotus root, and one plant to the south, and one plant northwards, sows the lotus root head of field surrounding, and direction is broadcast without exception
In kind field, a ridge a line, line-spacing 1-1.2m, hill spacing 0.6-0.8m；
The thimerosal is 10-15g/l Artemisia capillaris extracting solution, 8-15g/l Dalmatian chrysanthemums extracting solution, the extraction of 5-10g/l leek
Liquid, 5-10g/l balsamine extracting solutions；
S2, field management：
A applies top dressing：2 top dressings are carried out in the growth period of non-irrigated lotus root, is occurring carrying out when 3-5 pieces found leaf in first time, apply
Fertilizer amount is 800-1000kg per acre；Second of top dressing is carried out when tying lotus root, and dose is 800-1000kg per acre；
Fertilising for the first time is selenium-rich fertilizer, and selenium-rich fertilizer is prepared in percentage by weight by following raw material：10%-20%
Black bean powder, 10%-20% black glutinous rices, 10%-15% Fructus Hordei Germinatus, 10%-15% nutgrass flatsedges, 10%-15% selenium-enriched garlics, 8%-
15% k-selenocarrageenan, 5%-10% hearts, 5%-10% discard culture medium for golden mushroom, 1%-3% Se-enriched yeasts, 1%-3%
Lactobacillus acidophilus, 1%-3% bacillus megateriums, the thermophilic bacillus of 1%-3% heat；
Second fertilising is organic fertilizer, organic fertilizer in parts by weight, including 50-100 parts of bagasse, weathered coal 100-
200 parts, non-irrigated 50-100 parts of lotus root cauline leaf, 5-15 parts of potassium nitrate, 20-50 parts of chitosan, 10-20 parts of magnesium phosphate, medical stone 20-50
Part, 30-50 parts of vinasse, 20-40 parts of cripple, 0.1-0.5 parts of black yeast, 0.1-0.5 parts of cellulose-decomposing bacterium, Trichoderma 0.1-
0.5 part；
B weedings：It is carried out when there are weeds within 20-30 days after kind of lotus root is planted, the weeds pulled up is buried in the earth；
C is retained：It is retained using irrigation system, keeps plantation field humidity in 80%-90%；
S2, harvesting：Start to dig lotus root in the 3-4 months, digging lotus root first half of the month is drained.
It further illustrates, the Artemisia capillaris extracting solution is crushed after drying Artemisia capillaris, and use quality score is 8-15%
Alcohol solution dipping taken out after 2-5 minutes, then by sediment be added 3-8 times of volume water be heated to 35 degrees Celsius after stop
Then heating is that 500-800W is microwave irradiation 5-10 minutes and microwave irradiation dissolves while stirring using power, then passes through
Microfiltration takes filtrate to can be obtained Artemisia capillaris extracting solution；The Dalmatian chrysanthemum extracting solution is the distillation that Dalmatian chrysanthemum is added to 2 times of volumes
Water, which blends, to be slurried, and it is 1- to take 10 milliliters of slurries addition mass concentration is 65-80% for the first time 30 milliliters of ethyl alcohol and mass concentration
5-10 milliliters of 3% glycine-HCI buffer solution is stirred 5-10 minutes after standing 5-10 minutes and filters to take filtrate, takes filter
30 milliliters of the ethyl alcohol of second of addition of liquid a concentration of 75% and 5 milli of glycine-HCI buffer solution that mass concentration is 1-3%
It rises, is stirred 10-15 minutes after standing 5-10 minutes and filters to take filtrate, you can obtain Dalmatian chrysanthemum extracting solution；The leek carries
It according to volume ratio is 1-2 that take liquid, which be that temperature is added after cleaning leek is 35-45 degrees Celsius of hot water,:10 mixing, are put into diafiltration column
Middle cycle diafiltration, diafiltration flow velocity are maintained at 8-10ml/min, and extraction time 4-5 hour is then allowed to stand cooling, filters to take filter
Liquid, you can obtain leek extracts；The balsamine extracting solution is to dry balsamine to moisture content to carry out ultra micro later for 5%
It crushes, the ethanol solution that mass concentration is 65-85% is then added and carries out water-bath refluxing extraction 3 times, outer progress ultrasonic wave auxiliary
The filtrate of 3 extraction filterings is merged and can be obtained balsamine extracting solution by extraction.
It further illustrates, Se-enriched yeast, lactobacillus acidophilus, bacillus megaterium, the thermophilic brood cell's bar of heat in the selenium-rich fertilizer
Bacterium also passes through acclimation and screening and handles；The Se-enriched yeast acclimation and screening is handled：Chlorine is added in 0.01 square centimeter of Se-enriched yeast
Change sodium 0.5g/L, selenoaminoacid 0.5g/L, selenoprotein 0.8g/L, yeast powder 8g/L, Corn silk P.E 10g/L, black fungus to carry
Take object 15g/L, the activation culture of distilled water 1L, pH=6.5 composition shaking table culture 22-28 hours lower based on 28 degrees Celsius, then
Will it is living go out culture after bacterium solution to take 10mL that domestication culture medium A is added in temperature be that 32 degrees Celsius of lower shaking table cultures are sieved after 28-30 days
Choosing obtains, and tames the formula of culture medium A and is：Sodium selenite 5g/L, KH2PO4 3g/L、K2HPO4 5g/L、MgSO4.7H2O 3g/
L、CaSO4.2H2O 5g/L, Corn silk P.E 10g/L, black fungus extract 15g/L, distilled water 1L；The lactobacillus acidophilus
Acclimation and screening is handled：0.01 square centimeter of lactobacillus acidophilus is added containing 100 milliliters of domestication culture medium B then in high speed
It is shaken on whirlpool oscillator, screens and obtain after carrying out shaking table culture 20 hours under being 25 degrees Celsius in temperature, domestication culture medium B's
Formula is：Industrial wastewater 5mL, selenomethionine 5g/L, yeast extract 3g/L, Corn silk P.E 3g/L, black fungus extract 5g/
L, Tween-80 1g/L, KH2PO42g/L, sodium acetate 5g/L, lemon acid diamine 0.2g/L, distilled water 1L, then use quality is dense
Degree is 10% sodium carbonate or 10% salt acid for adjusting pH to 6.5；The bacillus megaterium acclimation and screening is handled：By 0.01 square
Centimetre bacillus megaterium is added shaking table culture in the domestication culture medium C containing 100 milliliters and can screen within 48 hours to obtain；It is described
Taming the formula of culture medium C is：The nutrient solution 7g/ of selenium mineral powder 0.1g/L, urea 2g/L, potassium dihydrogen phosphate 5g/L, inorganic elements
L, give up syrup 5mL, Corn silk P.E 3g/L, black fungus extract 5g/L, distilled water 1L in sugar refinery, and then use quality is a concentration of
10% sodium carbonate or 10% salt acid for adjusting pH are to 7；The thermophilic bacillus acclimation and screening of heat is handled：By 0.01 square centimeter of heat
Thermophilic bacillus is added oxygen content in the domestication culture medium D containing 100 milliliters and successively decreases per hour 10% culture 12 hours
Screening obtains, and the formula of the domestication culture medium D is：Give up syrup 5mL, Corn silk P.E 3g/L, black fungus extract in sugar refinery
5g/L、K2HPO4 5g/L、MgSO4.7H2O 3g/L, distilled water 1L, then a concentration of 10% sodium carbonate of use quality or 10% salt
Acid for adjusting pH is to 6.5.
Further instruction, Corn silk P.E are by the alcohol solution dipping 5 of corn stigma use quality a concentration of 15%
Minute, and passing through microwave radiation exaraction simultaneously, then microwave power recycles ethyl alcohol for 200-300W, mulberry leaf are added 2-5 times
Volume distilled water carries out heating and refluxing extraction 1-3 hours in U-tube, takes filtrate, then continues to repeat to heat by filter residue
Refluxing extraction 2-5 hours, takes filtrate, and filtrate twice is merged, distilled water, the 0.1-0.2 of 5-10 times of volume are then added
The cellulase of times volume heats while stirring 5-15 minutes, then stop heating standing it is cooling after use three layers of filtered through gauze,
It takes filtrate to be concentrated into 1.12 times of elementary volume and then can be obtained Corn silk P.E after being spray-dried；Wherein corn stigma carries
It takes and contains 95% or more corn silk polysaccharide in object.
Further instruction, black fungus extract are to pass through the distilled water that 4 times of volumes are added after black fungus solarization dry grinding
Ultrasonic extraction 5 minutes, supersonic frequency 100-120KHZ is subsequently added into after chitosan stirring is stood for 1 minute and proceeds by solid-liquid
Separation takes filtrate, and it is 1 that weight ratio will be added in filtrate:1-2:Cellulase, protease and the amylase of 2-3 is hydrolyzed, so
After filtrate is obtained by filtration, concentrate the filtrate to after 1.01 times of original volume that the ethanol solution stirring that mass concentration is 70% is added is quiet
It sets, via hole diameter is that 1-3 μm of membrane filtration goes filtrate, is then passed through filtrate and is impregnated by 0.02mol/L phosphate buffers
Ion exchange column afterwards is filtered, and the flow velocity that filtrate is passed through ion exchange column is 0.2-0.3mL/min, then passes through filtrate
Freeze-drying or vacuum drying or forced air drying obtain black fungus extract, more containing black fungus wherein in black fungus extract
95% or more sugar.
It further illustrates, the method for making that the selenium-rich fertilizer is specifically is：It, will be black glutinous after raw material is weighed according to percentage
Meter Jin Hang ultramicro grindings then calcine discarded culture medium for golden mushroom under 1000-1200 degrees Celsius of high temperature, then with other originals
Material is mixed, and builds heap fermentation and can be obtained within 5-8 days selenium-rich fertilizer.
It further illustrates, the selenium-rich fertilizer is prepared in percentage by weight by following raw material：15% black bean powder,
15% black glutinous rice, 12% Fructus Hordei Germinatus, 12% nutgrass flatsedge, 12% selenium-enriched garlic, 10% k-selenocarrageenan, 8% heart, 8% discarded gold
Needle mushroom culture medium, 2% Se-enriched yeast, 2% lactobacillus acidophilus, 2% bacillus megaterium, the 2% thermophilic bacillus of heat.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
1. the applicant by research find using independent research selenium-rich fertilizer, content by 10%-20% black bean powders,
10%-20% black glutinous rices, 10%-15% Fructus Hordei Germinatus, 10%-15% nutgrass flatsedges, 10%-15% selenium-enriched garlics, 8%-15% selenic acids
Polysaccharide ester, 5%-10% hearts, 5%-10% discard culture medium for golden mushroom, 1%-3% Se-enriched yeasts, 1%-3% acidophilus breast bars
The thermophilic bacillus composition of bacterium, 1%-3% bacillus megateriums, 1%-3% heat, wherein lactobacillus acidophilus, bacillus megaterium, heat
Thermophilic the bacillus ability with more excellent resistance adverse circumstances, energy when decomposing raw material organic matter after acclimation and screening
Accelerate the resolving time and decompose macromolecular substances to be that small-molecule substance is more abundant, improves decomposition efficiency, be conducive to plant absorption
Nutrient, and the trace element-selenium element content for decomposing release is up to 620 μ g/Kg, and non-irrigated lotus root absorbs during growth and development
It can also be improved using the utilization rate of selenium element, the utilization rate of absorption reaches 65-70%, and non-irrigated lotus root selenium amount is 29-34 micrograms every 100
Gram, which reaches level of security as defined in food, and edible safety is reliable.It applies fertilizer for the second time in the fertilizer used containing a large amount of
Organic matter, phosphorus, nitrogen, potassium element, calcium constituent, magnesium element content are high, promote growth after non-irrigated lotus root growth period can be promoted to absorb, improve
Phosphorus, nitrogen in non-irrigated lotus root, potassium element, calcium constituent, magnesium element content, and then promote the quality of non-irrigated lotus root.
2. applicant have also found that after carrying out disinfection prior to seeding to non-irrigated lotus root seed, there is the good energy for resisting pest and disease damage
Power, compounded in thimerosal 10-15g/l Artemisia capillaris extracting solution, 8-15g/l Dalmatian chrysanthemums extracting solution, 5-10g/l leek extracts,
5-10g/l balsamine extracting solutions, use plant extraction liquid first reduces toxicity to substitute chemical substance, has preferably peace
Quan Xing.There is good bactericidal effect, additional Dalmatian chrysanthemum to contain Dalmatian chrysanthemum for volatile oil, alkaloid contained by Artemisia capillaris extracting solution first
Plain first, second and cinerin first, second have good insecticidal effect, but property is unstable, it cannot be guaranteed that the effect of desinsection, adds mattress
Volatile oil contained by old wormwood artemisia extracting solution can enhance the insecticidal effect of Dalmatian chrysanthemum extracting solution, but its content cannot be excessively high, excessively high not rise
Insecticidal effect can be reduced instead to synergistic effect, contain sulfide, alkaloid, glucoside in leek extracts, balsamine extracting solution
Deng two kinds of substances of compounding can expand the range of desinsection, and leek extracts have good adhesion strength, promote thimerosal can be very
Good is adhered on the soil squirted, and the thimerosal researched and developed using the applicant is safe and non-toxic, have good insecticidal effect and
The desinsection time is lasting.
【Specific implementation mode】
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Embodiment is closed, the present invention is further explained.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention,
It is not intended to limit the present invention.
Embodiment 1：
A kind of cultural method improving non-irrigated lotus root quality, concrete operations are as follows：
S1, plantation：The rhizoma nelumbinis for selecting a section long and with 3 buds is sowed as seed, is soaked using thimerosal
Bubble, using after distilled water flushing, can be sowed after 3 minutes after dipping root-inducing powder, by row sowing from sowing field side 1.5m
Plant, arrangement will be interacted by planting the direction of lotus root, and one plant to the south, and one plant northwards, sows the lotus root head of field surrounding, without exception towards sowing field
In ground, a ridge a line, line-spacing 1m, hill spacing 0.6m；
The thimerosal is 10g/l Artemisia capillaris extracting solution, 8g/l Dalmatian chrysanthemums extracting solution, 5g/l leek extracts, 5g/l phoenix
Celestial being flower extracting solution；
S2, field management：
A applies top dressing：2 top dressings are carried out in the growth period of non-irrigated lotus root, carries out, applies fertilizer when there are 3 vertical leaves in first time
Amount is 800kg per acre；Second of top dressing is carried out when tying lotus root, and dose is 800kg per acre；
Fertilising for the first time is selenium-rich fertilizer, and selenium-rich fertilizer is prepared in percentage by weight by following raw material：20% black soya bean
Powder, 20% black glutinous rice, 10% Fructus Hordei Germinatus, 10% nutgrass flatsedge, 10% selenium-enriched garlic, 8% k-selenocarrageenan, 5% heart, 5% are discarded
Culture medium for golden mushroom, 3% Se-enriched yeast, 3% lactobacillus acidophilus, 3% bacillus megaterium, the 3% thermophilic bacillus of heat；
Second fertilising is organic fertilizer, organic fertilizer in parts by weight, including 50 parts of bagasse, 100 parts of weathered coal, drought
50 parts of lotus root cauline leaf, 5 parts of potassium nitrate, 20 parts of chitosan, 10 parts of magnesium phosphate, 20 parts of medical stone, 30 parts of vinasse, 20 parts of cripple, black ferment
0.1 part female, 0.1 part of cellulose-decomposing bacterium, 0.1 part of Trichoderma；
B weedings：It is carried out when there are weeds within 20 days after kind of lotus root is planted, the weeds pulled up is buried in the earth；
C is retained：It is retained using irrigation system, keeps plantation field humidity 80%；
S2, harvesting：Start to dig lotus root March, digging lotus root first half of the month is drained.
It further illustrates, the Artemisia capillaris extracting solution is crushed after drying Artemisia capillaris, the second that use quality score is 8%
Alcoholic solution takes out after impregnating 2 minutes, stops heating after the water of sediment 3 times of volumes of addition is then heated to 35 degrees Celsius, so
The use of power is afterwards that 500W is microwave irradiation 5 minutes and microwave irradiation dissolves while stirring, then takes filtrate i.e. by microfiltration
Artemisia capillaris extracting solution can be obtained；The Dalmatian chrysanthemum extracting solution is to blend the distilled water of Dalmatian chrysanthemum 2 times of volumes of addition to be slurried, and takes slurry
The glycine-HCI buffering that 30 milliliters of the ethyl alcohol that mass concentration is 65% is added in 10 milliliters of liquid for the first time and mass concentration is 1%
5 milliliters of liquid is stirred to stand for 5 minutes and filters to take filtrate after five minutes, takes the ethyl alcohol of second of addition of filtrate a concentration of 75%
30 milliliters are 1% 5 milliliters of glycine-HCI buffer solution with mass concentration, are stirred to stand for 10 minutes and filter to take after five minutes
Filtrate, you can obtain Dalmatian chrysanthemum extracting solution；The leek extracts are that temperature is added after cleaning leek as 35 degrees Celsius of hot water
It is 1 according to volume ratio:10 mixing are put into diafiltration column and recycle diafiltration, and diafiltration flow velocity is maintained at 8ml/min, and extraction time 4 is small
When, it is then allowed to stand cooling, filters to take filtrate, you can obtain leek extracts；The balsamine extracting solution is to dry balsamine
Ultramicro grinding is carried out after being 5% to moisture content, the ethanol solution that then addition mass concentration is 65% carries out water-bath reflux and carries
It takes 3 times, outer progress ultrasonic wave assisted extraction, the filtrate of 3 extraction filterings is merged and can be obtained balsamine extracting solution.
It further illustrates, Se-enriched yeast, lactobacillus acidophilus, bacillus megaterium, the thermophilic brood cell's bar of heat in the selenium-rich fertilizer
Bacterium also passes through acclimation and screening and handles；The Se-enriched yeast acclimation and screening is handled：Chlorine is added in 0.01 square centimeter of Se-enriched yeast
Change sodium 0.5g/L, selenoaminoacid 0.5g/L, selenoprotein 0.8g/L, yeast powder 8g/L, Corn silk P.E 10g/L, black fungus to carry
Take object 15g/L, distilled water 1L, the activation cultures of pH=6.5 compositions is based on 28 degrees Celsius of lower shaking table cultures 22 hours, will then live
It is that 32 degrees Celsius of lower shaking table cultures are screened after 28 days and obtained that the bacterium solution gone out after culture, which takes 10mL that domestication culture medium A is added in temperature,
Taming the formula of culture medium A is：Sodium selenite 5g/L, KH2PO4 3g/L、K2HPO4 5g/L、MgSO4.7H2O 3g/L、
CaSO4.2H2O 5g/L, Corn silk P.E 10g/L, black fungus extract 15g/L, distilled water 1L；The lactobacillus acidophilus is tamed and dociled
Changing Screening Treatment is：0.01 square centimeter of lactobacillus acidophilus is added containing 100 milliliters of domestication culture medium B then in high speed whirlpool
It is shaken on the oscillator of whirlpool, screens and obtain after carrying out shaking table culture 20 hours under being 25 degrees Celsius in temperature, domestication culture medium B's matches
Fang Shi：Industrial wastewater 5mL, selenomethionine 5g/L, yeast extract 3g/L, Corn silk P.E 3g/L, black fungus extract 5g/L,
Tween-80 1g/L, KH2PO42g/L, sodium acetate 5g/L, lemon acid diamine 0.2g/L, distilled water 1L, then use quality concentration
For 10% sodium carbonate or 10% salt acid for adjusting pH to 6.5；The bacillus megaterium acclimation and screening is handled：By 0.01 square li
Rice bacillus megaterium is added shaking table culture in the domestication culture medium C containing 100 milliliters and can screen within 48 hours to obtain；It is described to tame and docile
Changing the formula of culture medium C is：Selenium mineral powder 0.1g/L, urea 2g/L, potassium dihydrogen phosphate 5g/L, inorganic elements nutrient solution 7g/L,
Give up syrup 5mL, Corn silk P.E 3g/L, black fungus extract 5g/L, distilled water 1L in sugar refinery, and then use quality is a concentration of
10% sodium carbonate or 10% salt acid for adjusting pH are to 7；The thermophilic bacillus acclimation and screening of heat is handled：By 0.01 square centimeter of heat
Thermophilic bacillus is added oxygen content in the domestication culture medium D containing 100 milliliters and successively decreases per hour 10% culture 12 hours
Screening obtains, and the formula of the domestication culture medium D is：Give up syrup 5mL, Corn silk P.E 3g/L, black fungus extract in sugar refinery
5g/L、K2HPO4 5g/L、MgSO4.7H2O 3g/L, distilled water 1L, then a concentration of 10% sodium carbonate of use quality or 10% salt
Acid for adjusting pH is to 6.5.
Further instruction, Corn silk P.E are by the alcohol solution dipping 5 of corn stigma use quality a concentration of 15%
Minute, and passing through microwave radiation exaraction simultaneously, then microwave power recycles ethyl alcohol for 200W, 2 times of volumes, which are added, in mulberry leaf steams
Distilled water carries out heating and refluxing extraction 1 hour in U-tube, takes filtrate, then continues to filter residue repeating heating and refluxing extraction 2
Hour, filtrate is taken, filtrate twice is merged, then adds the distilled water of 5 times of volumes, the cellulase side of 0.1 times of volume
It heats side to stir 5 minutes, then stops using three layers of filtered through gauze after heating standing is cooling, filtrate is taken to be concentrated into elementary volume
1.12 times be then spray-dried after can be obtained Corn silk P.E；Wherein contain corn silk polysaccharide in Corn silk P.E
95% or more.
Further instruction, black fungus extract are to pass through the distilled water that 4 times of volumes are added after black fungus solarization dry grinding
Ultrasonic extraction 5 minutes, supersonic frequency 100KHZ is subsequently added into after chitosan stirring is stood for 1 minute and proceeds by separation of solid and liquid
Filtrate is taken, it is 1 that weight ratio will be added in filtrate:1:2 cellulase, protease and amylase is hydrolyzed, and then filters
To filtrate, the ethanol solution stirring standing that mass concentration is 70% is added after concentrating the filtrate to 1.01 times of original volume, through hole
Diameter is that 1 μm of membrane filtration goes filtrate, then hands over the ion that filtrate is passed through after the immersion of 0.02mol/L phosphate buffers
It changes column to be filtered, the flow velocity that filtrate is passed through ion exchange column is 0.2mL/min, then by the freeze-drying of filtrate process or very
Empty drying or forced air drying obtain black fungus extract, and 95% or more Blackfungus polyhexose is contained wherein in black fungus extract.
It further illustrates, the method for making that the selenium-rich fertilizer is specifically is：It, will be black glutinous after raw material is weighed according to percentage
Meter Jin Hang ultramicro grindings then calcine discarded culture medium for golden mushroom under 1000 degrees Celsius of high temperature, then mixed with other raw materials
Stirring is closed, heap fermentation is built and can be obtained within 5 days selenium-rich fertilizer.
Embodiment 2：
A kind of cultural method improving non-irrigated lotus root quality, concrete operations are as follows：
S1, plantation：The rhizoma nelumbinis for selecting a section long and with 5 buds is sowed as seed, is soaked using thimerosal
It after bubble uses distilled water flushing after five minutes, can be sowed after dipping root-inducing powder, by row sowing from sowing field side 1.5m
Plant, arrangement will be interacted by planting the direction of lotus root, and one plant to the south, and one plant northwards, sows the lotus root head of field surrounding, without exception towards sowing field
In ground, a ridge a line, line-spacing 1.2m, hill spacing 0.8m；
The thimerosal is 15g/l Artemisia capillaris extracting solution, 15g/l Dalmatian chrysanthemums extracting solution, 10g/l leek extracts, 10g/l
Balsamine extracting solution；
S2, field management：
A applies top dressing：3 top dressings are carried out in the growth period of non-irrigated lotus root, carries out, applies fertilizer when there are 5 vertical leaves in first time
Amount is 1000kg per acre；Second of top dressing is carried out when tying lotus root, and dose is 1000kg per acre；
Fertilising for the first time is selenium-rich fertilizer, and selenium-rich fertilizer is prepared in percentage by weight by following raw material：10% black soya bean
Powder, 10% black glutinous rice, 15% Fructus Hordei Germinatus, 15% nutgrass flatsedge, 15% selenium-enriched garlic, 15% k-selenocarrageenan, 5%% heart, 5% give up
Abandon culture medium for golden mushroom, 3% Se-enriched yeast, 2 lactobacillus acidophilus, 2% bacillus megaterium, the 3% thermophilic bacillus of heat；
Second fertilising is organic fertilizer, organic fertilizer in parts by weight, including 100 parts of bagasse, 200 parts of weathered coal,
100 parts of non-irrigated lotus root cauline leaf, 15 parts of potassium nitrate, 50 parts of chitosan, 20 parts of magnesium phosphate, 50 parts of medical stone, 50 parts of vinasse, 40 parts of cripple,
0.5 part of black yeast, 0.5 part of cellulose-decomposing bacterium, 0.5 part of Trichoderma；
B weedings：It is carried out when there are weeds within 30 days after kind of lotus root is planted, the weeds pulled up is buried in the earth；
C is retained：It is retained using irrigation system, keeps plantation field humidity 90%；
S2, harvesting：Start to dig lotus root April, digging lotus root first half of the month is drained.
It further illustrates, the Artemisia capillaris extracting solution is crushed after drying Artemisia capillaris, and use quality score is 15%
Alcohol solution dipping takes out after five minutes, stops heating after the water of sediment 8 times of volumes of addition is then heated to 35 degrees Celsius,
Then the use of power is that 800W is microwave irradiation 10 minutes and microwave irradiation dissolves while stirring, then takes filtrate by microfiltration
It can be obtained Artemisia capillaris extracting solution；The Dalmatian chrysanthemum extracting solution is to blend the distilled water of Dalmatian chrysanthemum 2 times of volumes of addition to be slurried, and is taken
30 milliliters of the ethyl alcohol that mass concentration is 80% is added in 10 milliliters of slurries for the first time and the glycine-HCI that mass concentration is 3% is slow
10 milliliters of fliud flushing is stirred to stand for 10 minutes and filters to take filtrate after ten minutes, takes second of addition of filtrate a concentration of 75%
30 milliliters of ethyl alcohol and mass concentration be 3% 5 milliliters of glycine-HCI buffer solution, be stirred 15 minutes and stand 10 minutes
After filter to take filtrate, you can obtain Dalmatian chrysanthemum extracting solution；The leek extracts are that temperature is added after cleaning leek to take the photograph for 45
Family name's degree hot water is 2 according to volume ratio:10 mixing are put into diafiltration column and recycle diafiltration, and diafiltration flow velocity is maintained at 10ml/min, carries
It takes the time 5 hours, is then allowed to stand cooling, filters to take filtrate, you can obtain leek extracts；The balsamine extracting solution is by phoenix
Celestial being spends the dry ethanol solution progress water for carrying out ultramicro grinding later for 5% to moisture content, being then added that mass concentration is 85%
Bathe refluxing extraction 3 times, the filtrate of 3 extraction filterings is merged and can be obtained balsamine extracting solution by outer progress ultrasonic wave assisted extraction.
It further illustrates, Se-enriched yeast, lactobacillus acidophilus, bacillus megaterium, the thermophilic brood cell's bar of heat in the selenium-rich fertilizer
Bacterium also passes through acclimation and screening and handles；The Se-enriched yeast acclimation and screening is handled：Chlorine is added in 0.01 square centimeter of Se-enriched yeast
Change sodium 0.5g/L, selenoaminoacid 0.5g/L, selenoprotein 0.8g/L, yeast powder 8g/L, Corn silk P.E 10g/L, black fungus to carry
Take object 15g/L, distilled water 1L, the activation cultures of pH=6.5 compositions is based on 28 degrees Celsius of lower shaking table cultures 28 hours, will then live
It is that 32 degrees Celsius of lower shaking table cultures are screened after 30 days and obtained that the bacterium solution gone out after culture, which takes 10mL that domestication culture medium A is added in temperature,
Taming the formula of culture medium A is：Sodium selenite 5g/L, KH2PO4 3g/L、K2HPO4 5g/L、MgSO4.7H2O 3g/L、
CaSO4.2H2O 5g/L, Corn silk P.E 10g/L, black fungus extract 15g/L, distilled water 1L；The lactobacillus acidophilus is tamed and dociled
Changing Screening Treatment is：0.01 square centimeter of lactobacillus acidophilus is added containing 100 milliliters of domestication culture medium B then in high speed whirlpool
It is shaken on the oscillator of whirlpool, screens and obtain after carrying out shaking table culture 20 hours under being 25 degrees Celsius in temperature, domestication culture medium B's matches
Fang Shi：Industrial wastewater 5mL, selenomethionine 5g/L, yeast extract 3g/L, Corn silk P.E 3g/L, black fungus extract 5g/L,
Tween-80 1g/L, KH2PO42g/L, sodium acetate 5g/L, lemon acid diamine 0.2g/L, distilled water 1L, then use quality concentration
For 10% sodium carbonate or 10% salt acid for adjusting pH to 6.5；The bacillus megaterium acclimation and screening is handled：By 0.01 square li
Rice bacillus megaterium is added shaking table culture in the domestication culture medium C containing 100 milliliters and can screen within 48 hours to obtain；It is described to tame and docile
Changing the formula of culture medium C is：Selenium mineral powder 0.1g/L, urea 2g/L, potassium dihydrogen phosphate 5g/L, inorganic elements nutrient solution 7g/L,
Give up syrup 5mL, Corn silk P.E 3g/L, black fungus extract 5g/L, distilled water 1L in sugar refinery, and then use quality is a concentration of
10% sodium carbonate or 10% salt acid for adjusting pH are to 7；The thermophilic bacillus acclimation and screening of heat is handled：By 0.01 square centimeter of heat
Thermophilic bacillus is added oxygen content in the domestication culture medium D containing 100 milliliters and successively decreases per hour 10% culture 12 hours
Screening obtains, and the formula of the domestication culture medium D is：Give up syrup 5mL, Corn silk P.E 3g/L, black fungus extract in sugar refinery
5g/L、K2HPO4 5g/L、MgSO4.7H2O 3g/L, distilled water 1L, then a concentration of 10% sodium carbonate of use quality or 10% salt
Acid for adjusting pH is to 6.5.
Further instruction, Corn silk P.E are by the alcohol solution dipping 5 of corn stigma use quality a concentration of 15%
Minute, and passing through microwave radiation exaraction simultaneously, then microwave power recycles ethyl alcohol for 300W, 5 times of volumes, which are added, in mulberry leaf steams
Distilled water carries out heating and refluxing extraction 3 hours in U-tube, takes filtrate, then continues to filter residue repeating heating and refluxing extraction 5
Hour, filtrate is taken, filtrate twice is merged, then adds the distilled water of 10 times of volumes, the cellulase side of 0.2 times of volume
It heats side to stir 15 minutes, then stops using three layers of filtered through gauze after heating standing is cooling, filtrate is taken to be concentrated into elementary volume
1.12 times be then spray-dried after can be obtained Corn silk P.E；Wherein contain corn silk polysaccharide in Corn silk P.E
95% or more.
Further instruction, black fungus extract are to pass through the distilled water that 4 times of volumes are added after black fungus solarization dry grinding
Ultrasonic extraction 5 minutes, supersonic frequency 120KHZ is subsequently added into after chitosan stirring is stood for 1 minute and proceeds by separation of solid and liquid
Filtrate is taken, it is 1 that weight ratio will be added in filtrate:2:3 cellulase, protease and amylase is hydrolyzed, and then filters
To filtrate, the ethanol solution stirring standing that mass concentration is 70% is added after concentrating the filtrate to 1.01 times of original volume, through hole
Diameter is that 3 μm of membrane filtration goes filtrate, then hands over the ion that filtrate is passed through after the immersion of 0.02mol/L phosphate buffers
It changes column to be filtered, the flow velocity that filtrate is passed through ion exchange column is 0.3mL/min, then by the freeze-drying of filtrate process or very
Empty drying or forced air drying obtain black fungus extract, and 95% or more Blackfungus polyhexose is contained wherein in black fungus extract.
It further illustrates, the method for making that the selenium-rich fertilizer is specifically is：It, will be black glutinous after raw material is weighed according to percentage
Meter Jin Hang ultramicro grindings then calcine discarded culture medium for golden mushroom under 1200 degrees Celsius of high temperature, then mixed with other raw materials
Stirring is closed, heap fermentation is built and can be obtained within 8 days selenium-rich fertilizer.
Embodiment 3：
A kind of cultural method improving non-irrigated lotus root quality, concrete operations are as follows：
S1, plantation：The rhizoma nelumbinis for selecting a section long and with 4 buds is sowed as seed, is soaked using thimerosal
Bubble, using after distilled water flushing, can be sowed after 4 minutes after dipping root-inducing powder, by row sowing from sowing field side 1.5m
Plant, arrangement will be interacted by planting the direction of lotus root, and one plant to the south, and one plant northwards, sows the lotus root head of field surrounding, without exception towards sowing field
In ground, a ridge a line, line-spacing 1.1m, hill spacing 0.7m；
The thimerosal is 12g/l Artemisia capillaris extracting solution, 11g/l Dalmatian chrysanthemums extracting solution, 7g/l leek extracts, 8g/l phoenix
Celestial being flower extracting solution；
S2, field management：
A applies top dressing：2 top dressings are carried out in the growth period of non-irrigated lotus root, carries out, applies fertilizer when there are 4 vertical leaves in first time
Amount is 900kg per acre；Second of top dressing is carried out when tying lotus root, and dose is 900kg per acre；
Fertilising for the first time is selenium-rich fertilizer, and selenium-rich fertilizer is prepared in percentage by weight by following raw material：The selenium-rich
Fertilizer is prepared in percentage by weight by following raw material：15% black bean powder, 15% black glutinous rice, 12% Fructus Hordei Germinatus, 12% Sha
It is grass, 12% selenium-enriched garlic, 10% k-selenocarrageenan, 8% heart, 8% discarded culture medium for golden mushroom, 2% Se-enriched yeast, 2% thermophilic
Lactobacillus lactis, 2% bacillus megaterium, the 2% thermophilic bacillus of heat；
Second fertilising is organic fertilizer, organic fertilizer in parts by weight, including 80 parts of bagasse, 120 parts of weathered coal, drought
85 parts of lotus root cauline leaf, 10 parts of potassium nitrate, 35 parts of chitosan, 15 parts of magnesium phosphate, 40 parts of medical stone, 40 parts of vinasse, 30 parts of cripple, black ferment
0.13 part female, 0.2 part of cellulose-decomposing bacterium, 0.4 part of Trichoderma；
B weedings：It is carried out when there are weeds within 28 days after kind of lotus root is planted, the weeds pulled up is buried in the earth；
C is retained：It is retained using irrigation system, keeps plantation field humidity 85%；
S2, harvesting：Start to dig lotus root March, digging lotus root first half of the month is drained.
It further illustrates, the Artemisia capillaris extracting solution is crushed after drying Artemisia capillaris, and use quality score is 10%
Alcohol solution dipping takes out after 3 minutes, stops heating after the water of sediment 5 times of volumes of addition is then heated to 35 degrees Celsius,
Then the use of power is that 700W is microwave irradiation 7 minutes and microwave irradiation dissolves while stirring, then takes filtrate by microfiltration
It can be obtained Artemisia capillaris extracting solution；The Dalmatian chrysanthemum extracting solution is to blend the distilled water of Dalmatian chrysanthemum 2 times of volumes of addition to be slurried, and is taken
30 milliliters of the ethyl alcohol that mass concentration is 70% is added in 10 milliliters of slurries for the first time and the glycine-HCI that mass concentration is 2% is slow
8 milliliters of fliud flushing is stirred 8 minutes after standing 7 minutes and filters to take filtrate, takes the second of second of addition of filtrate a concentration of 75%
5 milliliters of the glycine-HCI buffer solution that 30 milliliters of alcohol and mass concentration are 2% is stirred 12 minutes after standing 6 minutes and filters
Take filtrate, you can obtain Dalmatian chrysanthemum extracting solution；The leek extracts are that temperature is added after cleaning leek as 40 degrees Celsius of heat
Water is 1 according to volume ratio:10 mixing are put into diafiltration column and recycle diafiltration, and diafiltration flow velocity is maintained at 9ml/min, extraction time
It 4.5 hours, is then allowed to stand cooling, filters to take filtrate, you can obtain leek extracts；The balsamine extracting solution is by balsamine
It is dry to carry out ultramicro grinding later to moisture content for 5%, the ethanol solution progress water-bath time that mass concentration is 70% is then added
The filtrate of 3 extraction filterings is merged and can be obtained balsamine extracting solution by stream extraction 3 times, outer progress ultrasonic wave assisted extraction.
It further illustrates, Se-enriched yeast, lactobacillus acidophilus, bacillus megaterium, the thermophilic brood cell's bar of heat in the selenium-rich fertilizer
Bacterium also passes through acclimation and screening and handles；The Se-enriched yeast acclimation and screening is handled：Chlorine is added in 0.01 square centimeter of Se-enriched yeast
Change sodium 0.5g/L, selenoaminoacid 0.5g/L, selenoprotein 0.8g/L, yeast powder 8g/L, Corn silk P.E 10g/L, black fungus to carry
Take object 15g/L, distilled water 1L, the activation cultures of pH=6.5 compositions is based on 28 degrees Celsius of lower shaking table cultures 25 hours, will then live
It is that 32 degrees Celsius of lower shaking table cultures are screened after 29 days and obtained that the bacterium solution gone out after culture, which takes 10mL that domestication culture medium A is added in temperature,
Taming the formula of culture medium A is：Sodium selenite 5g/L, KH2PO4 3g/L、K2HPO4 5g/L、MgSO4.7H2O 3g/L、
CaSO4.2H2O 5g/L, Corn silk P.E 10g/L, black fungus extract 15g/L, distilled water 1L；The lactobacillus acidophilus is tamed and dociled
Changing Screening Treatment is：0.01 square centimeter of lactobacillus acidophilus is added containing 100 milliliters of domestication culture medium B then in high speed whirlpool
It is shaken on the oscillator of whirlpool, screens and obtain after carrying out shaking table culture 20 hours under being 25 degrees Celsius in temperature, domestication culture medium B's matches
Fang Shi：Industrial wastewater 5mL, selenomethionine 5g/L, yeast extract 3g/L, Corn silk P.E 3g/L, black fungus extract 5g/L,
Tween-80 1g/L, KH2PO42g/L, sodium acetate 5g/L, lemon acid diamine 0.2g/L, distilled water 1L, then use quality concentration
For 10% sodium carbonate or 10% salt acid for adjusting pH to 6.5；The bacillus megaterium acclimation and screening is handled：By 0.01 square li
Rice bacillus megaterium is added shaking table culture in the domestication culture medium C containing 100 milliliters and can screen within 48 hours to obtain；It is described to tame and docile
Changing the formula of culture medium C is：Selenium mineral powder 0.1g/L, urea 2g/L, potassium dihydrogen phosphate 5g/L, inorganic elements nutrient solution 7g/L,
Give up syrup 5mL, Corn silk P.E 3g/L, black fungus extract 5g/L, distilled water 1L in sugar refinery, and then use quality is a concentration of
10% sodium carbonate or 10% salt acid for adjusting pH are to 7；The thermophilic bacillus acclimation and screening of heat is handled：By 0.01 square centimeter of heat
Thermophilic bacillus is added oxygen content in the domestication culture medium D containing 100 milliliters and successively decreases per hour 10% culture 12 hours
Screening obtains, and the formula of the domestication culture medium D is：Give up syrup 5mL, Corn silk P.E 3g/L, black fungus extract in sugar refinery
5g/L、K2HPO4 5g/L、MgSO4.7H2O 3g/L, distilled water 1L, then a concentration of 10% sodium carbonate of use quality or 10% salt
Acid for adjusting pH is to 6.5.
Further instruction, Corn silk P.E are by the alcohol solution dipping 5 of corn stigma use quality a concentration of 15%
Minute, and passing through microwave radiation exaraction simultaneously, then microwave power recycles ethyl alcohol for 250W, 3 times of volumes, which are added, in mulberry leaf steams
Distilled water carries out heating and refluxing extraction 2 hours in U-tube, takes filtrate, then continues to filter residue repeating heating and refluxing extraction 4
Hour, filtrate is taken, filtrate twice is merged, then adds the distilled water of 8 times of volumes, the cellulase side of 0.1 times of volume
It heats side to stir 9 minutes, then stops using three layers of filtered through gauze after heating standing is cooling, filtrate is taken to be concentrated into elementary volume
1.12 times be then spray-dried after can be obtained Corn silk P.E；Wherein contain corn silk polysaccharide in Corn silk P.E
95% or more.
Further instruction, black fungus extract are to pass through the distilled water that 4 times of volumes are added after black fungus solarization dry grinding
Ultrasonic extraction 5 minutes, supersonic frequency 110KHZ is subsequently added into after chitosan stirring is stood for 1 minute and proceeds by separation of solid and liquid
Filtrate is taken, it is 1 that weight ratio will be added in filtrate:2:2 cellulase, protease and amylase is hydrolyzed, and then filters
To filtrate, the ethanol solution stirring standing that mass concentration is 70% is added after concentrating the filtrate to 1.01 times of original volume, through hole
Diameter is that 2 μm of membrane filtration goes filtrate, then hands over the ion that filtrate is passed through after the immersion of 0.02mol/L phosphate buffers
It changes column to be filtered, the flow velocity that filtrate is passed through ion exchange column is 0.3mL/min, then by the freeze-drying of filtrate process or very
Empty drying or forced air drying obtain black fungus extract, and 95% or more Blackfungus polyhexose is contained wherein in black fungus extract.
It further illustrates, the method for making that the selenium-rich fertilizer is specifically is：It, will be black glutinous after raw material is weighed according to percentage
Meter Jin Hang ultramicro grindings then calcine discarded culture medium for golden mushroom under 1100 degrees Celsius of high temperature, then mixed with other raw materials
Stirring is closed, heap fermentation is built and can be obtained within 7 days selenium-rich fertilizer.
Comparative example 1：
Cultural method is same as Example 3, difference be selenium-rich fertilizer prepared in percentage by weight by following raw material and
At：15% black bean powder, 15% black glutinous rice, 12% Fructus Hordei Germinatus, 12% nutgrass flatsedge, 12% selenium-enriched garlic, 10% k-selenocarrageenan, 8%
Heart, 8% discarded culture medium for golden mushroom, 2% Se-enriched yeast, 2% lactobacillus acidophilus, 2% bacillus megaterium, the 2% thermophilic bud of heat
Born of the same parents bacillus, but Se-enriched yeast, lactobacillus acidophilus, bacillus megaterium, the thermophilic bacillus of heat are handled without acclimation and screening.
Comparative example 2：
Cultural method is same as Example 3, difference be selenium-rich fertilizer prepared in percentage by weight by following raw material and
At：15% black bean powder, 15% black glutinous rice, 12% Fructus Hordei Germinatus, 12% nutgrass flatsedge, 12% selenium-enriched garlic, 10% k-selenocarrageenan, 8%
Heart, 8% discarded culture medium for golden mushroom, 8% Se-enriched yeast.
Comparative example 3：
Cultural method is same as Example 3, and difference is that thimerosal is not used to carry out plantation disinfection.
Comparative example 4：
Cultural method is same as Example 3, and difference is thimerosal composition：18g/l Artemisia capillaris extracting solution, 11g/l deinsectization
Chrysanthemum extracting solution, 8g/l leek extracts, 7g/l balsamine extracting solutions.
Comparative example 5：
The remaining fertilising of cultural method is composite fertilizer of the conventional cultivation method fertilising using purchase.
Experiment 1：The planting patterns of embodiment 1-3 and comparative example 1-5 is tested with cultivating 8 pieces of non-irrigated lotus fields respectively, often
Block is planted according to embodiment 1-3 with comparative example 1-5 respectively.Work as harvesting, it will be in the embodiment of the present invention 1-3 and comparative example 1-5
It is random that 3 pieces of seeds is taken to be used as experimental example, survey soluble protein content, soluble sugar content, Vitamin C content, ammonia nitrogen content,
Se content.Wherein：Content of soluble protein is measured using Coomassie brilliant G-250 method；Flavones content content is using light splitting light
Degree method measures；Moisture is measured using 1995 methods of GB/T3543.6-；Content of starch uses GB5009.9-2008 methods；
Se content is measured with reference to national standard GB5009.93-2010, and measurement result is shown in Table 1；
Table 1 (average ± standard deviation n=3)
The upper right mark difference letter of each numerical value represents the significance of difference (P in upper table<0.05)
As seen from the above table, the quality for the non-irrigated lotus root that planting patterns using the present invention obtains is more excellent.
Table 2
As seen from the above table, thimerosal using the present invention, with drug effect, more longlasting, insecticidal range is wider, adhesion strength is more preferable,
The more preferable advantage of control effect.
Table 3
As seen from the above table, thimerosal using the present invention has the effect of excellent preventing disease and pest.
By the non-irrigated lotus root of the embodiment 1-3 harvested and comparative example 5, randomly selects 3 non-irrigated lotus roots and carry out following nutrient
It measures, mensuration mode is national standard mode, specifically be see the table below：
4 picking fruit nutrient composition content of table
As seen from the above table, the non-irrigated lotus root for planting out using the application method, the amino acid content contained in non-irrigated lotus root are superior to
General planting method, and coordinate the non-irrigated lotus root N P and K calcium and magnesium member that organic fat material is planted using the selenium-enriched fertilizer of the application
Cellulose content is improved, and to sum up, the quality for the non-irrigated lotus root that the present processes obtain is more excellent.
Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Cannot limitation of the scope of the invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention
It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (6)
1. a kind of cultural method improving non-irrigated lotus root quality, it is characterised in that：Concrete operations are as follows：
S1, plantation：The rhizoma nelumbinis for selecting a section long and with 3-5 bud is sowed as seed, is impregnated using thimerosal
It using after distilled water flushing after 3-5 minutes, can be sowed after dipping root-inducing powder, by row sowing from sowing field side 1.5m
Plant, arrangement will be interacted by planting the direction of lotus root, and one plant to the south, and one plant northwards, sows the lotus root head of field surrounding, without exception towards sowing field
In ground, a ridge a line, line-spacing 1-1.2m, hill spacing 0.6-0.8m；
The thimerosal is 10-15g/l Artemisia capillaris extracting solution, 8-15g/l Dalmatian chrysanthemums extracting solution, 5-10g/l leek extracts, 5-
10g/l balsamine extracting solutions；
S2, field management：
A applies top dressing：2 top dressings are carried out in the growth period of non-irrigated lotus root, are occurring carrying out when 3-5 pieces found leaf in first time, dose
For 800-1000kg per acre；Second of top dressing is carried out when tying lotus root, and dose is 800-1000kg per acre；
Fertilising for the first time is selenium-rich fertilizer, and selenium-rich fertilizer is prepared in percentage by weight by following raw material：10%-20% black soya beans
Powder, 10%-20% black glutinous rices, 10%-15% Fructus Hordei Germinatus, 10%-15% nutgrass flatsedges, 10%-15% selenium-enriched garlics, 8%-15% selenium
Acid esters polysaccharide, 5%-10% hearts, 5%-10% discard culture medium for golden mushroom, 1%-3% Se-enriched yeasts, 1%-3% acidophilus breasts
Bacillus, 1%-3% bacillus megateriums, the thermophilic bacillus of 1%-3% heat；
Second fertilising is organic fertilizer, organic fertilizer in parts by weight, including 50-100 parts of bagasse, weathered coal 100-200
Part, non-irrigated 50-100 parts of lotus root cauline leaf, 5-15 parts of potassium nitrate, 20-50 parts of chitosan, 10-20 parts of magnesium phosphate, 20-50 parts of medical stone, wine
30-50 parts poor, 20-40 parts of cripple, 0.1-0.5 parts of black yeast, 0.1-0.5 parts of cellulose-decomposing bacterium, 0.1-0.5 parts of Trichoderma；
B weedings：It is carried out when there are weeds within 20-30 days after kind of lotus root is planted, the weeds pulled up is buried in the earth；
C is retained：It is retained using irrigation system, keeps plantation field humidity in 80%-90%；
S2, harvesting：Start to dig lotus root in the 3-4 months, digging lotus root first half of the month is drained.
2. a kind of cultural method improving non-irrigated lotus root quality according to claim 1, it is characterised in that：The Artemisia capillaris extraction
Liquid is crushed after drying Artemisia capillaris, and use quality score is that the alcohol solution dipping of 8-15% takes out after 2-5 minutes, then will
The water of sediment 3-8 times of volume of addition stops heating after being heated to 35 degrees Celsius, the use of power is then microwave for 500-800W
It irradiates 5-10 minutes and microwave irradiation dissolves while stirring, then take filtrate to can be obtained Artemisia capillaris extracting solution by microfiltration；
The Dalmatian chrysanthemum extracting solution is to blend the distilled water of Dalmatian chrysanthemum 2 times of volumes of addition to be slurried, and 10 milliliters of slurries is taken to be added for the first time
5-10 milliliters of the glycine-HCI buffer solution that 30 milliliters of the ethyl alcohol that mass concentration is 65-80% and mass concentration are 1-3%, into
Row stirring filters to take filtrate after standing 5-10 minutes within 5-10 minutes, takes 30 milli of ethyl alcohol of second of addition of filtrate a concentration of 75%
It rises and mass concentration is 5 milliliters of the glycine-HCI buffer solution of 1-3%, be stirred mistake after standing 5-10 minutes 10-15 minutes
Leaching filtrate, you can obtain Dalmatian chrysanthemum extracting solution；The leek extracts are that addition temperature is that 35-45 is Celsius after cleaning leek
It is 1-2 to spend hot water according to volume ratio:10 mixing are put into diafiltration column and recycle diafiltration, and diafiltration flow velocity is maintained at 8-10ml/min,
Extraction time 4-5 hour is then allowed to stand cooling, filters to take filtrate, you can obtains leek extracts；The balsamine extracting solution is
Balsamine is dried to moisture content and carries out ultramicro grinding later for 5%, the ethanol solution that mass concentration is 65-85% is then added
Carry out water-bath refluxing extraction 3 times, the filtrate of 3 extraction filterings is merged and can be obtained phoenix celestial being by outer progress ultrasonic wave assisted extraction
Flower extracting solution.
3. a kind of cultural method improving non-irrigated lotus root quality according to claim 1, it is characterised in that：In the selenium-rich fertilizer
Se-enriched yeast, lactobacillus acidophilus, bacillus megaterium, the thermophilic bacillus of heat also pass through acclimation and screening and handle；The Se-enriched yeast
Acclimation and screening is handled：Sodium chloride 0.5g/L, selenoaminoacid 0.5g/L, selenoprotein is added in 0.01 square centimeter of Se-enriched yeast
0.8g/L, yeast powder 8g/L, Corn silk P.E 10g/L, black fungus extract 15g/L, distilled water 1L, what pH=6.5 was formed
Activation culture is shaking table culture 22-28 hours lower based on 28 degrees Celsius, and the bacterium solution after the culture that then will live in takes 10mL that domestication is added
Culture medium A is that 32 degrees Celsius of lower shaking table cultures are screened after 28-30 days and obtained in temperature, and taming the formula of culture medium A is：Selenous acid
Sodium 5g/L, KH2PO4 3g/L、K2HPO4 5g/L、MgSO4.7H2O 3g/L、CaSO4.2H2O 5g/L, Corn silk P.E 10g/
L, black fungus extract 15g/L, distilled water 1L；The lactobacillus acidophilus acclimation and screening is handled：By 0.01 square centimeter of acidophilus
Then lactobacillus is added to be shaken containing 100 milliliters of domestication culture medium B on high speed whirlpool oscillator, be 25 degrees Celsius in temperature
Lower progress shaking table culture is screened after 20 hours to be obtained, and the formula of domestication culture medium B is：Industrial wastewater 5mL, selenomethionine 5g/
L, yeast extract 3g/L, Corn silk P.E 3g/L, black fungus extract 5g/L, Tween-80 1g/L, KH2PO42g/L, sodium acetate
5g/L, lemon acid diamine 0.2g/L, distilled water 1L, then a concentration of 10% sodium carbonate of use quality or 10% salt acid for adjusting pH arrive
6.5；The bacillus megaterium acclimation and screening is handled：0.01 square centimeter of bacillus megaterium is added and contains 100 milliliters
Domestication culture medium C in shaking table culture can screen within 48 hours to obtain；It is described domestication culture medium C formula be：Selenium mineral powder 0.1g/
L, urea 2g/L, potassium dihydrogen phosphate 5g/L, the nutrient solution 7g/L of inorganic elements, the useless syrup 5mL in sugar refinery, Corn silk P.E 3g/
L, black fungus extract 5g/L, distilled water 1L, then a concentration of 10% sodium carbonate of use quality or 10% salt acid for adjusting pH to 7；
The thermophilic bacillus acclimation and screening of heat is handled：The thermophilic bacillus of 0.01 square centimeter of heat is added and is tamed and dociled containing 100 milliliters
Changing 10% culture of successively decreasing per hour of oxygen content in culture medium D can screen to obtain for 12 hours, the formula of the domestication culture medium D
It is：Give up syrup 5mL, Corn silk P.E 3g/L, black fungus extract 5g/L, K in sugar refinery2HPO4 5g/L、MgSO4.7H2O 3g/
L, distilled water 1L, then a concentration of 10% sodium carbonate of use quality or 10% salt acid for adjusting pH to 6.5.
4. a kind of cultural method improving non-irrigated lotus root quality according to claim 3, it is characterised in that：Corn silk P.E is
By the alcohol solution dipping 5 minutes of corn stigma use quality a concentration of 15%, and pass through microwave radiation exaraction, microwave power simultaneously
For 200-300W, ethyl alcohol is then recycled, mulberry leaf 2-5 times of volume distilled water of addition is subjected to heating and refluxing extraction in U-tube
1-3 hours, filtrate is taken, then continues to filter residue repeating heating and refluxing extraction 2-5 hours, takes filtrate, by filtrate twice
Merge, the cellulase of the distilled water, 0.1-0.2 times of volume that then add 5-10 times of volume heats while stirring 5-15 points
Then clock stops using three layers of filtered through gauze after heating standing cooling, filtrate is taken to be concentrated into 1.12 times of then progress of elementary volume
It can be obtained Corn silk P.E after spray drying；Black fungus extract is that 4 times of volumes are added after black fungus is shone dry grinding
Distilled water by ultrasonic extraction 5 minutes, supersonic frequency 100-120KHZ, be subsequently added into chitosan stir 1 minute stand after open
Beginning, which be separated by solid-liquid separation, takes filtrate, and it is 1 that weight ratio will be added in filtrate:1-2:Cellulase, protease and the amylase of 2-3 into
Row hydrolysis, is then obtained by filtration filtrate, and the ethyl alcohol that mass concentration is 70% is added after concentrating the filtrate to 1.01 times of original volume
Solution stirring is stood, and via hole diameter is that 1-3 μm of membrane filtration goes filtrate, and then filtrate is passed through by 0.02mol/L phosphate
Ion exchange column after buffer solution impregnates is filtered, and the flow velocity that filtrate is passed through ion exchange column is 0.2-0.3mL/min, then
Filtrate is obtained into black fungus extract by freeze-drying or vacuum drying or forced air drying.
5. a kind of cultural method improving non-irrigated lotus root quality according to claim 1, it is characterised in that：The selenium-rich fertilizer is specific
The method for making for being is：After raw material is weighed according to percentage, black glutinous rice is subjected to ultramicro grinding, then trains discarded needle mushroom
It supports base to calcine under 1000-1200 degrees Celsius of high temperature, be then mixed with other raw materials, build heap and ferment 5-8 days and can be obtained
Selenium-rich fertilizer.
6. a kind of cultural method improving non-irrigated lotus root quality according to claim 1, it is characterised in that：The selenium-rich fertilizer by with
Lower raw material is prepared in percentage by weight：15% black bean powder, 15% black glutinous rice, 12% Fructus Hordei Germinatus, 12% nutgrass flatsedge, 12%
Selenium-enriched garlic, 10% k-selenocarrageenan, 8% heart, 8% discarded culture medium for golden mushroom, 2% Se-enriched yeast, 2% acidophilus breast bar
Bacterium, 2% bacillus megaterium, the 2% thermophilic bacillus of heat.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710766061.2A CN108293762A (en) | 2017-08-30 | 2017-08-30 | A kind of cultural method improving non-irrigated lotus root quality |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710766061.2A CN108293762A (en) | 2017-08-30 | 2017-08-30 | A kind of cultural method improving non-irrigated lotus root quality |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108293762A true CN108293762A (en) | 2018-07-20 |
Family
ID=62869640
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710766061.2A Pending CN108293762A (en) | 2017-08-30 | 2017-08-30 | A kind of cultural method improving non-irrigated lotus root quality |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108293762A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108929153A (en) * | 2018-07-24 | 2018-12-04 | 博白县百家水果专业合作社 | A kind of Citrus shatangju selenium-enriched fertilizer and preparation method thereof |
| CN111602486A (en) * | 2019-02-26 | 2020-09-01 | 贵州省亚热带作物研究所 | Seedling transplanting method for canna edulis ker seedling seeds |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102578160A (en) * | 2012-01-11 | 2012-07-18 | 陕西恒田化工有限公司 | Artemisia capillaris extract with antiviral and antifungal activity as well as application of artemisia capillaris extract |
| CN105340514A (en) * | 2015-10-09 | 2016-02-24 | 广西壮族自治区农业科学院经济作物研究所 | Efficient cultivation method for increasing yield and starch content of canna edulis |
| CN105660745A (en) * | 2016-03-03 | 2016-06-15 | 董志富 | Pesticide for controlling and preventing powdery mildew of traditional Chinese medicinal material Chinese parsnip root and preparation method thereof |
| CN106069171A (en) * | 2016-08-12 | 2016-11-09 | 广西鑫雅皇庭园林工程有限责任公司 | The implantation methods of drought Rhizoma Nelumbinis |
| CN106258261A (en) * | 2015-05-12 | 2017-01-04 | 梁燕 | A kind of Rhizoma Nelumbinis Hirudo hybrid breeding method |
| CN106576546A (en) * | 2016-11-16 | 2017-04-26 | 唐滨 | Plantation method of selenium-rich edible canna |
| CN106576736A (en) * | 2016-11-16 | 2017-04-26 | 唐滨 | High-yield planting method of edible canna rich in selenium |
| CN106717249A (en) * | 2016-11-16 | 2017-05-31 | 唐滨 | The implantation methods of rich strontium drought lotus root |
| CN106818344A (en) * | 2016-12-13 | 2017-06-13 | 卢志文 | Prevent the cultural method of rice blast |
| CN106977355A (en) * | 2017-06-08 | 2017-07-25 | 合肥慧明瀚生态农业科技有限公司 | A kind of Chinese herbaceous peony cultivation matrix and preparation method thereof |
-
2017
- 2017-08-30 CN CN201710766061.2A patent/CN108293762A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102578160A (en) * | 2012-01-11 | 2012-07-18 | 陕西恒田化工有限公司 | Artemisia capillaris extract with antiviral and antifungal activity as well as application of artemisia capillaris extract |
| CN106258261A (en) * | 2015-05-12 | 2017-01-04 | 梁燕 | A kind of Rhizoma Nelumbinis Hirudo hybrid breeding method |
| CN105340514A (en) * | 2015-10-09 | 2016-02-24 | 广西壮族自治区农业科学院经济作物研究所 | Efficient cultivation method for increasing yield and starch content of canna edulis |
| CN105660745A (en) * | 2016-03-03 | 2016-06-15 | 董志富 | Pesticide for controlling and preventing powdery mildew of traditional Chinese medicinal material Chinese parsnip root and preparation method thereof |
| CN106069171A (en) * | 2016-08-12 | 2016-11-09 | 广西鑫雅皇庭园林工程有限责任公司 | The implantation methods of drought Rhizoma Nelumbinis |
| CN106576546A (en) * | 2016-11-16 | 2017-04-26 | 唐滨 | Plantation method of selenium-rich edible canna |
| CN106576736A (en) * | 2016-11-16 | 2017-04-26 | 唐滨 | High-yield planting method of edible canna rich in selenium |
| CN106717249A (en) * | 2016-11-16 | 2017-05-31 | 唐滨 | The implantation methods of rich strontium drought lotus root |
| CN106818344A (en) * | 2016-12-13 | 2017-06-13 | 卢志文 | Prevent the cultural method of rice blast |
| CN106977355A (en) * | 2017-06-08 | 2017-07-25 | 合肥慧明瀚生态农业科技有限公司 | A kind of Chinese herbaceous peony cultivation matrix and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108929153A (en) * | 2018-07-24 | 2018-12-04 | 博白县百家水果专业合作社 | A kind of Citrus shatangju selenium-enriched fertilizer and preparation method thereof |
| CN111602486A (en) * | 2019-02-26 | 2020-09-01 | 贵州省亚热带作物研究所 | Seedling transplanting method for canna edulis ker seedling seeds |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105077029A (en) | Dinner replacing powder containing zinc-rich barley grass and preparation method of dinner replacing powder | |
| CN107382525A (en) | A kind of organic foliar fertilizer for improving non-irrigated lotus root quality | |
| CN107445769A (en) | A kind of tealeaves ecological fertilizer special | |
| CN104987156B (en) | A kind of method of binwang mushroom culture medium and cultivation binwang mushroom using mushroom bran | |
| CN103910569A (en) | Fruit tree specially-used biological fertilizer, and preparation method thereof | |
| CN101781133A (en) | Organic selenium-rich pear special nutrient, preparing method and applications thereof | |
| CN100586905C (en) | Plants probiotics composite preparation and preparing technique thereof | |
| CN106278665A (en) | A kind of high-quality organic fertilizer | |
| CN103650899A (en) | Method enabling lucid ganoderma and conidial powder of lucid ganoderma to be rich in microelements | |
| CN106187633A (en) | A kind of fertilizer | |
| CN103804099A (en) | Peach tree fertilizer and preparation method thereof | |
| CN105768067A (en) | Enzyme with health care function and manufacturing method thereof | |
| CN106083394A (en) | A kind of method utilizing reed powder to make Enoki mushroom cultivation material | |
| CN103435421B (en) | Compatibility and production method of stropharia rugoso-annulata cultivation material | |
| CN106187634A (en) | A kind of Biocidal type fertilizer | |
| CN104402617A (en) | Pleurotus eryngii cultivation material with use of garlic straw and pleurotus eryngii production method | |
| CN106220355A (en) | A kind of fertilizer | |
| CN108293762A (en) | A kind of cultural method improving non-irrigated lotus root quality | |
| CN106220356A (en) | A kind of Biocidal type organic fertilizer | |
| CN106242891A (en) | A kind of Biocidal type fertilizer | |
| CN103483058B (en) | Formula and manufacturing method of coprinus comatus culture material | |
| CN107746304A (en) | A kind of Se-rich lucid ganoderma cultivation matrix and preparation method thereof | |
| CN107417470A (en) | A kind of soybean ecological fertilizer special | |
| CN107382529A (en) | A kind of okra ecological fertilizer special | |
| CN107580999A (en) | A kind of cultural method of environmentally friendly selenium-rich drought lotus root |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180720 |