CN102485878B - Multi-cell co-culture apparatus used in simulated weightless environment - Google Patents
Multi-cell co-culture apparatus used in simulated weightless environment Download PDFInfo
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- CN102485878B CN102485878B CN 201010574419 CN201010574419A CN102485878B CN 102485878 B CN102485878 B CN 102485878B CN 201010574419 CN201010574419 CN 201010574419 CN 201010574419 A CN201010574419 A CN 201010574419A CN 102485878 B CN102485878 B CN 102485878B
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Abstract
The invention provides a multi-cell co-culture apparatus used for research of simulated weightlessness. The apparatus comprises a culture bottle body, a culture bottle spiral cover, a semi-permeable membrane, a retaining piece and a cross-shaped support, wherein, the semi-permeable membrane, the retaining piece and the cross-shaped support are used for culturing cells and the culture bottle body, and the culture bottle spiral cover compose a multi-cell co-culture bottle and enable butt joint between the multi-cell co-culture apparatus and a cell simulated weightless device to be realized. The apparatus can be used for culture of a single kind of cells in a simulated weightless environment and for co-culture of two or more kinds of cells in a simulated weightless environment, fully takes influence of contact among cells and cell communication into consideration, can maximumly meet requirements for multi-cell co-culture in a simulated weightless environment, enables the disadvantage that conventional simulated weightless devices can only culture a single kind of cells to be overcome and is simple to operate and repeatedly usable.
Description
Affiliated technical field
The present invention relates to the many cells associating culture apparatus under a kind of Simulated Weightlessness.
Background technology
In the body situation, the regulation and control of body physiological function are not to be completed by the cell of single kind isolatedly, jointly realize but form complicated regulated and control network by various kinds of cell.Simultaneously, be subjected to the interaction of communication between the cytokine of emiocytosis and flanking cell, the function between the different tissues cell also influences each other.Therefore, the result that draws for a kind of isolatism institute of cell merely, can not reflect fully cell at the body functional status, even draw and cell conflicting result in the body situation.Yet, all be based on the design of cellar culture condition for cellulous associating culture technique at present, and lack the many cells associating culture apparatus under Simulated Weightlessness.Current, restriction due to device, simulated weightlessness research for cell levels also only rests on the conceptual phase of single kind cell, in the urgent need to inventing a kind of many cells joint study that culture apparatus is carried out weightless level of uniting, to obtain result of study accurately.Therefore, the large sample of development under a kind of Simulated Weightlessness, many cells associating culture apparatus easy and simple to handle are very necessary.
Summary of the invention
The simulated weightlessness research of existing cell levels is limited to the restriction of hardware condition, only can carry out the cell research of single kind, can't truly reflect cell at the body functional status.In order to overcome the deficiency of single kind cell research, the invention provides a kind of large sample, many cells easy and simple to handle and unite and cultivate the simulated weightlessness device.
The technical solution adopted for the present invention to solve the technical problems is: Multi-cell co-culture apparatus used in simulated weightless environment forms by cultivating bottle, culturing bottle spiral cover, semi-permeable membranes, holding piece and cross bracket five parts, semi-permeable membranes, holding piece and cross bracket are used for culturing cell, cultivate bottle, culturing bottle spiral cover composition many cells associating culturing bottle, and realization is simulated docking of zero gravity facility with cell.
The invention has the beneficial effects as follows: according to the difference of research purpose, this device both can carry out the cytology research of single kind, also can carry out two or more many cells research, thereby realize that the cell yield is high, easy and simple to handle, reusable, also reflect that accurately cell is in the purpose of body functional status.
The present invention is further described below in conjunction with accompanying drawing.
Fig. 1 shows the basic structure of Multi-cell co-culture apparatus used in simulated weightless environment.
1. culturing bottle spiral covers in Fig. 1 are 2. cultivated bottle, 3. holding piece, 4. semi-permeable membranes, 5. cross bracket.
Fig. 2 shows Multi-cell co-culture apparatus used in simulated weightless environment intraware figure.
A. semi-permeable membranes in Fig. 2, B. holding piece, C. cross bracket vertical view, D. cross bracket side bitmap.
Embodiment
In Fig. 1 and Fig. 2, holding piece (3) is rigid material, is divided into two pages in up and down, be hollow " mouth " font and be connected with axle, and two pages of necessarily damping up and down foldings, it act as semi-permeable membranes (4) is fixed between two pages.Cross bracket (5) is rigid structure, four ends of cross bracket (5) respectively have two chutes, the holding piece (3) that is fixed with semi-permeable membranes (4) can vertically insert in the chute of cross bracket (5) and the fixing sample rack that forms, each cross bracket (5) can insert at most four holding pieces (3), to realize the cultivation in enormous quantities of cell.Culturing bottle spiral cover (1) and cultivation bottle (2) are the transparent polystyrene injection moulding, cultivate bottle (2) and be hollow cylindrical, the cylindrical upper end opening also has outside screw, the lower end is airtight, culturing bottle spiral cover (1) has internal thread, can connect to form airtight complete culturing bottle structure with the outside screw of cultivating on bottle (2).Sample rack can be put into and cultivate bottle (2), can cross bracket (5) be fixed on the cultivation bottle by squeezing action inner after culturing bottle spiral cover (1) is tightened.Cultivate bottle (2) and can be clamped to the simulated weightlessness that realizes cell levels on cell simulation zero gravity facility.
For cells contacting under the research Simulated Weightlessness reaches closely cell communication to influencing each other between two kinds of different sorts attached cells, during use, semi-permeable membranes (4) is cut to suitable size, be fixed in up and down between two and half pages of holding piece (3), according to experiment purpose, two kinds of different types of attached cells obtain and go down to posterity.After stable the cultivation, with a kind of cell of 0.5% tryptic digestion, stop digesting and making cell suspension with containing serum free culture system liquid, with 1 * 10 before experiment
5The density of/ml is inoculated in the A face of semi-permeable membranes (4), after cell attachment, makes the suspension of another kind of cell, the upset semi-permeable membranes (4) to B facing up, with the another kind of purpose cell of equal densities inoculation, fully rear standby until cell attachment, each face is carried out mark again.The cross bracket (5) that disinfects is placed in cultivates bottle (2), fill it up with the cell culture fluid of 37 ℃ of preheatings, the above-mentioned holding piece for preparing (3) is inserted respectively in the end chute of cross bracket (5), each cross bracket (5) can be inserted at most four holding pieces (3).The piping and druming nutrient solution is tightened culturing bottle spiral cover (1), and is examined cell culture system to eliminate the bubble that adheres in culturing bottle, to cultivate in bottle (2) without bubble as qualified.To cultivate bottle (2) is fixed on cell simulation zero gravity facility and completes follow-up study work.
As for long distance communications such as research cytokine etc. to influencing each other between two kinds of different attached cells, can different types of cell single face is adherent on different semi-permeable membranes (4), carrying out mark is inserted on cross bracket (5), respectively insert two holding pieces (3) that contain the identical type cell on each cross bracket (5), be connected on cell simulation zero gravity facility after tightening culturing bottle spiral cover (1), complete follow-up test.
As for influencing each other between the research many cells, various types of cell is inoculated in respectively on different semi-permeable membranes (4), to carry out mark to insert on cross bracket (5), all the other steps are with above-mentioned.
Claims (1)
1. Multi-cell co-culture apparatus used in simulated weightless environment, be used for the simulated weightlessness research of cell levels, by culturing bottle spiral cover (1), cultivate bottle (2), holding piece (3), semi-permeable membranes (4) and cross bracket (5) five parts form, it is characterized by: after semi-permeable membranes (4) is fixed on holding piece (3), two-sidedly can respectively inoculate a kind of different cell, holding piece after inoculating cell (3) is inserted cross bracket (5), and change in cultivation bottle (2), be full of cell culture fluid, screw culturing bottle spiral cover (1), form an airtight complete many cells associating culture system, this device docks with cell simulation zero gravity facility, realize cellulous simulated weightlessness effect.
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CN 201010574419 CN102485878B (en) | 2010-12-01 | 2010-12-01 | Multi-cell co-culture apparatus used in simulated weightless environment |
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CN102485878A CN102485878A (en) | 2012-06-06 |
CN102485878B true CN102485878B (en) | 2013-05-15 |
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CN111040948B (en) * | 2020-01-13 | 2024-07-23 | 中国人民解放军第四军医大学 | Liquid changing device for multicellular co-culture simulated weightlessness experiment |
CN111040947B (en) * | 2020-01-13 | 2023-06-02 | 中国人民解放军第四军医大学 | Liquid-changing multicellular co-culture simulated weightlessness experiment device |
Citations (4)
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US5026650A (en) * | 1988-06-30 | 1991-06-25 | The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration | Horizontally rotated cell culture system with a coaxial tubular oxygenator |
CN1290744A (en) * | 1999-12-09 | 2001-04-11 | 中国科学院力学研究所 | Spacial dialyzing counterflow cell cultivator and cultivating method |
CN201195726Y (en) * | 2008-02-19 | 2009-02-18 | 中国航天员科研训练中心 | Combined cell culture bottle |
CN101560469A (en) * | 2008-04-14 | 2009-10-21 | 上海交通大学医学院附属上海儿童医学中心 | Rotary dual-chamber stem cell synchronous augmentation and division inoculator |
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JP5606008B2 (en) * | 2009-04-13 | 2014-10-15 | 三菱重工業株式会社 | Method for culturing bone marrow stromal cells and mesenchymal stem cells, method for producing transplanted cells for treatment of central nervous system diseases |
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5026650A (en) * | 1988-06-30 | 1991-06-25 | The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration | Horizontally rotated cell culture system with a coaxial tubular oxygenator |
CN1290744A (en) * | 1999-12-09 | 2001-04-11 | 中国科学院力学研究所 | Spacial dialyzing counterflow cell cultivator and cultivating method |
CN201195726Y (en) * | 2008-02-19 | 2009-02-18 | 中国航天员科研训练中心 | Combined cell culture bottle |
CN101560469A (en) * | 2008-04-14 | 2009-10-21 | 上海交通大学医学院附属上海儿童医学中心 | Rotary dual-chamber stem cell synchronous augmentation and division inoculator |
Non-Patent Citations (2)
Title |
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失重状态下共育大鼠成骨与破骨细胞中护骨素表达的变化;尹文哲等;《中国临床康复》;20050521;第9卷(第19期);第174-175页 * |
尹文哲等.失重状态下共育大鼠成骨与破骨细胞中护骨素表达的变化.《中国临床康复》.2005,第9卷(第19期),第174-175页. |
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