CN107494710A - Lentinan combines application of the fludioxonil in preventing and treating fruit, vegetable storage disease - Google Patents
Lentinan combines application of the fludioxonil in preventing and treating fruit, vegetable storage disease Download PDFInfo
- Publication number
- CN107494710A CN107494710A CN201710796782.8A CN201710796782A CN107494710A CN 107494710 A CN107494710 A CN 107494710A CN 201710796782 A CN201710796782 A CN 201710796782A CN 107494710 A CN107494710 A CN 107494710A
- Authority
- CN
- China
- Prior art keywords
- fludioxonil
- lentinan
- fruit
- component
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses application of the lentinan joint fludioxonil in preventing and treating fruit, vegetable storage disease, the disease is the microbial grape grey mould of Botrytis cinerea, garlic stems gray mold.During concrete application, bactericidal composition is made in lentinan and fludioxonil compounding, after being diluted with water, sprinkling or dipping are in fruit, vegetable surface, after drying, freezer normal storage；In bactericidal composition, the weight ratio of lentinan and fludioxonil is 1~10:1~15, preferably 1:1~10.Also disclose a kind of antisepsis antistaling agent：Lentinan 0.5%~15%, fludioxonil 0.5%~50%, surplus are to allow to use and acceptable auxiliary material in Pesticide Science.The present invention has advantages below：(1) obvious synergistic function；(2) usage amount and access times of chemical bactericide are reduced；(3) drug combination of the different mechanisms of action uses, and delays the generation of fungicide resistance；(4) corrosion-resistanting fresh-keeping effect of fruit and vegetable food is more notable.
Description
Technical field
The present invention relates to lentinan joint fludioxonil in preventing and treating fruit, the application in vegetable storage disease, belong to fruit,
Vegetables postharvest disease prevention and control field.
Background technology
Fresh agricultural products storage logistics is the continuity of agricultural production, and it is people's pursuit to keep its excellent quality and freshness
One of important goal, rotten postharvest decay is that must be solved the problems, such as in fresh agricultural products storage transport, logistics progress.According to system
Meter, the loss late in developed country's veterinary antibiotics product transporting procedures less than 5%, and the annual loss late in China be up to 25%~
30%.The pathogen of Botrytis cinerea (Botrytis cinerea) is an important pathogen for causing postharvest decay rotten, can endanger Portugal
The various fruits such as grape, strawberry, peach, apple, pears, tomato, cucumber, eggplant, leek, vegetables, not only caused sternly in fruits and vegetables growth period
Endanger again, and the important disease of storage period.
Grey grape disease conventional chemical bactericide such as carbendazim, diethofencarb, iprodione, pyrimethanil etc. are prevented and treated in production, but
The pathogen of Botrytis cinerea has the characteristics that breeding is fast, hereditary variation is big, grade of fit is high, and same medicament is used continuously and is also easy to produce the resistance to the action of a drug,
Extreme difficulties are brought to preventing and treating, only control fruit and vegetable food to rot by increasing dosing or times for spraying, it is either right
Still ensure that fruits and vegetables quality is all unfavorable in improving antiseptic effect.Meanwhile with the concern of the food-safe problem of people, kill
The residual toxicity of microbial inoculum is increasingly taken seriously, and finds the few germ killing drugs combination of safer, wide spectrum, dosage, reductionization
The usage amount of bactericide is learned, delays drug-fast generation, is the study hotspot of current postharvest fruit and vegetable anti-corrosive fresh-keeping, and the present invention
Technical problems to be solved.
Lentinan (Lentiman), molecular formula：C42H72O36, it is true as caused by the Basidiomycetes Agaricaceae of Pleurotaceae
The poly- polysaccharide of mushroom, based on glucose, containing a small amount of mannose, fucose, galactolipin, xylose, arabinose etc..Lentinan
Field of medicaments is applied to earliest, to improve body immunity, anticancer, treatment malignant tumour and antiviral.But led in agricultural
Domain equally has good anti-phytoviral activity, and is applied to production.Lentinan also has disease-resistant, bacteriostatic activity, energy
Plant is enough induced to produce and accumulate the disease resistance materials such as salicylic acid, protective plant protecting agent and chitinase.There are some researches show lentinan
Crop can not only be induced to produce good induction of resistance to disease, also there is certain inhibitory action to phytopathogen, together
When can improve the anti-adversity ability of crop；The bactericidal activity that can improve medicament mixed with bactericide.
Fludioxonil (fludioxonil) is a kind of non-internal-absorting phenylpyrrole series bactericidal agent, and it is pseudomonas
Secondary metabolites pyrrolnitrin caused by (the Pseudomonas pyrrocinia) not of the same race of (Pseudomonas spp.)
(pyrrolnitrin) analog, due to its solution more anti-light than natural products pyrrolnitrin, mould can be suppressed in specific manner and
The fungal disease being widely used in preventing and treating agricultural production.Fludioxonil can both suppress spore rudiment, bacterium germ tube extends,
Ash arrhizus bacteria mycelial growth, chain sclerotinia sclerotiorum belong (Monilinia spp.), Sclerotinia can be effective against again
The fungies such as (Sclerotinia spp.), penicillium expansum (Penicillium expansum), to sac fungus, basidiomycetes, partly know
The pathogens such as bacterium have good preventive effect.
Lentinan is used alone prevented and control fruit, vegetable storage phase gray mold, penicilliosis effect are paid no attention to
Think.Have not yet to see about lentinan is combined to prevent the report for fruits and vegetables storage period disease of harnessing the river with fludioxonil.
The content of the invention
For above-mentioned prior art, the invention provides a kind of preventing and treating fruit, the new method of vegetable storage phase disease ---
By lentinan and fludioxonil use in conjunction.
The present invention is achieved by the following technical solutions：
Lentinan combines application of the fludioxonil in preventing and treating fruit, vegetable storage disease.
Preferably, the fruit is selected from grape, cherry, blueberry, strawberry, peach, apple, pears；The vegetables are selected from tomato, Huang
Melon, eggplant, leek, garlic stems.
Preferably, the disease is the microbial disease of Botrytis cinerea, such as grape grey mould, garlic stems gray mold.
Further, during concrete application, bactericidal composition is made in lentinan and fludioxonil compounding, after being diluted with water,
Sprinkling or dipping are in fruit, vegetable surface, after drying, freezer normal storage；In bactericidal composition, lentinan is with coughing up bacterium
The weight ratio of nitrile is 1~10:1~15, preferably 1:1~10.
Preferably, the bactericidal composition is made up of the component of following parts by weight：Lentinan 0.5%~15%,
Fludioxonil 0.5%~50%, surplus are to allow to use and acceptable auxiliary material in Pesticide Science；In use, it is diluted with water 1000
~2000 times.
Further, during concrete application, the preparation using lentinan as active component (during concrete application, can be led to respectively
Diluted on site mushroom polysaccharide mother liquor is crossed to obtain), using fludioxonil as active component preparation (during concrete application, can by scene it is dilute
Fludioxonil preparation is released to obtain), sprinkling or dipping are in fruit, vegetable surface, after drying, freezer normal storage.
A kind of antisepsis antistaling agent, it is the component group by following parts by weight for the anti-corrosive fresh-keeping of the storage period of water fruits and vegetables
Into：Lentinan 0.5%~15%, fludioxonil 0.5%~50%, surplus are to allow to use and acceptable in Pesticide Science
Auxiliary material.
Allow to use in the Pesticide Science and acceptable auxiliary material is selected from solvent, dispersant, diffusant, disintegrant, breast
Agent, adsorbent, stabilizer, wetting agent, defoamer, antifreezing agent etc. and other stabilizations for contributing to active ingredient drug effect and performance
Allow use auxiliary agent.
The antisepsis antistaling agent, it can be prepared into what suitable agricultural production used with processing method well known in the prior art
Any one formulation, preferred dosage form are：Wettable powder, suspending agent, aqueous emulsion, water dispersible granules, microemulsion.
A kind of preservation method of water fruits and vegetables (preventing and treating fruit, the method for vegetable storage phase disease)：By lentinan with coughing up
Bactericidal composition is made in bacterium nitrile compounding, and after being diluted with water, sprinkling or dipping are in fruit, vegetable surface, and after drying, freezer is routinely store
Tibetan；In bactericidal composition, the weight ratio of lentinan and fludioxonil is 1~10:1~15, preferably 1:1~10；
Or：During concrete application, the preparation using lentinan as active component (during concrete application, can be passed through into scene respectively
Dilution mushroom polysaccharide mother liquor obtain), the preparation using fludioxonil as active component (during concrete application, bacterium can be coughed up by diluted on site
Nitrile preparation obtains), sprinkling or dipping are in fruit, vegetable surface, after drying, freezer normal storage.
Preferably, the bactericidal composition is made up of the component of following parts by weight：Lentinan 0.5%~15%,
Fludioxonil 0.5%~50%, surplus are to allow to use and acceptable auxiliary material in Pesticide Science.
The present inventor is found by experiment that, lentinan and fludioxonil are compounded, and has advantages below：
(1) obvious synergistic function, said composition improve bactericidal activity of the fludioxonil to the pathogen of Botrytis cinerea；(2) reducing
The usage amount and access times of bactericide are learned, reduces the use cost of fludioxonil, improves the quality safety of food；(3) it is different
The drug combination of the mechanism of action uses, and delays the generation of fungicide resistance；(4) corrosion-resistanting fresh-keeping effect of fruit and vegetable food is more aobvious
Write, substantially prolongs the shelf life of fruit and vegetable food, improve the economic value of fruit and vegetable food.
Embodiment
With reference to embodiment, the present invention is further illustrated.
Involved instrument, reagent, material etc. in following embodiments, it is existing in the prior art unless otherwise noted
Conventional instrument, reagent, material etc., can be obtained by regular commercial sources.Involved experimental method in following embodiments, inspection
Survey method etc., it is existing normal experiment method, detection method etc. in the prior art unless otherwise noted.
Test lentinan and the united prevention effect of fludioxonil
First, reagent agent：4% mushroom polysaccharide mother liquor, 95% fludioxonil active compound, by fludioxonil active compound acetone solution, and
10000 μ g/mL mother liquor is made into, emulsifying agent Tween-80 is added with 0.2% volume fraction, stored in refrigerator at 4 DEG C standby
Debita spissitudo is diluted to sterilized water with during, use.
2nd, for trying pathogen：The pathogen of Botrytis cinerea Botrytis cinerea.
3rd, Toxicity Determination method：
Lentinan, fludioxonil and the two different ratio are combined using mycelial growth rate method and carry out toxicity test, and
Calculate co-toxicity coefficient (CTC).
Single dose toxicity test method：
Reagent agent (including fludioxonil, pyrimethanil) is first configured to 5 concentration gradient (fludioxonils：0.5、1.0、2.0、
4.0th, 5 concentration gradients such as 8.0mg/kg；Pyrimethanil：12.5th, 25.0,50.0,100.0,5 concentration ladders such as 200.0mg/kg
Degree), by the pathogen of Botrytis cinerea in 26 DEG C of preculture 3d in PDA culture medium, with diameter 7mm card punch close to the same of colony edge
Beaten on one circumference and take bacteria cake, be inoculated on the culture medium flat plate containing different agents concentration, it is a piece of per ware, it is placed in 26 DEG C of insulating boxs
Interior culture 3d, the colony growth diameter of each processing is determined with crossing method, using clear water as control, processing is repeated 4 times per concentration.
Bacterium colony radially linear increment is determined, determines inhibiting rate of the medicament to colony growth.Probable value and medicine are suppressed by mycelial growth
Linear regression analysis between agent concentration logarithm value, each medicament is obtained to concentration (EC in effective suppression of bacterial strain50Value).Mixed agent
Joint toxicity measuring：
On the basis of single dose toxicity test, lentinan, fludioxonil for mixture are pressed into its EC respectively50Value dosage presses body
Product ratio sets 10 respectively:1、5:1、3:1、2:1、1:1、1:2、1:3、1:5、1:10、1:15 totally 10 proportioning, by different ratio
Be configured to final mass concentration for 0.25,0.5,1.0,2.0, the drug containing PDA culture medium of 5 series concentrations of 4.0mg/kg, measure
Inhibiting rate of the medicament to mycelial growth.Often processing is repeated 4 times (per bacterial strain per concentration level).Probable value is suppressed by mycelial growth
Linear regression analysis between drug concentration logarithm value, Mixed Pharmacy is obtained to concentration EC in effective suppression of bacterial strain50Value and
CTC values, control is used as using the PDA culture medium of not adding medicine.
4th, data statistic analysis
Test data carries out statistical analysis by Microsoft Excel 2013, DPS data processing works platform, calculates
Go out the EC of every kind of medicament50Value, 95% confidence limit.The co-toxicity coefficient of mixed agent is calculated using the abundant methods of Sun Yun (1960), according to common poison
Coefficient magnitude evaluates the synergistic effect of mixed agent.
Each medicament inhibiting rate is calculated according to below equation：
Suppress growth rate (%)=[(control antibacterial circle diameter-processing antibacterial circle diameter)/(control antibacterial circle diameter -5mm
Punch diameter)] × 100；
Suppress growth rate (%)=[(control colony diameter-processing colony diameter)/(control colony diameter-bacteria cake diameter)]
×100；
Toxicity index TI=(standard agent EC50/ reagent agent EC50)×100；
Actual toxicity index ATI=(the standard agent EC of mixed agent50/ mixture preparation EC50)×100；
(PA and PB are respectively mixed agent to mixed agent theoretical toxicity index TTI=single doses A TI × PA+ single doses B TI × PB
The percentage composition of middle active ingredient)；
Theoretical toxicity index TTI × 100 of the actual toxicity index ATI/ mixed agents of co-toxicity coefficient CTC=mixed agents.
Synergistic effect judges：CTC >=120, there is synergistic effect；80 ＜ CTC ＜ 120, it is summation action；CTC≤80, it is
Antagonism.
5th, result and analysis
Toxicity test result of the lentinan of table 1 with fludioxonil different proportion mixture to the pathogen of Botrytis cinerea
From table 1, lentinan, fludioxonil and its mixture preparation, there is good inhibiting effect to the pathogen of Botrytis cinerea,
Wherein the proportioning of lentinan and fludioxonil is 1:1~1:When between 10, to for try pathogen co-toxicity coefficient 120 with
On, show that both compositions match down have good synergistic function herein, especially match 1:3~1:10 it
Between, EC50Value is respectively less than the EC that fludioxonil is used alone50Value 0.5829mg/kg, fludioxonil is significantly improved to the pathogen of Botrytis cinerea
Activity, for co-toxicity coefficient (CTC) value up to 135.71~147.82, synergistic effect is more notable.
The lentinan fludioxonil suspending agent of example of formulations 1 20%
Component composition is following (each component is by weight percentage)：
Preparation method is：Above-mentioned each active component, various auxiliary agents and water are sufficiently mixed uniformly in proportion, input is sanded
Machine, 5 μm of particle diameter is ground to, 20% lentinan fludioxonil suspending agent is made.
The lentinan fludioxonil wettable powder of example of formulations 2 40%
Component composition is following (each component is by weight percentage)：
Preparation method is：Above-mentioned each active component, various auxiliary agents and filler are sufficiently mixed uniformly in proportion, mixture warp
Ultra-fine pulverizer disintegrating, 40% lentinan fludioxonil wettable powder is made.
The lentinan fludioxonil aqueous emulsion of example of formulations 3 20%
Component composition is following (each component is by weight percentage)：
Preparation method is：Above-mentioned each active component, various auxiliary agents are sufficiently mixed in proportion, make to be dissolved into homogeneous oil phase,
Under high-speed stirred, oil phase is mixed with water, obtains 20% lentinan fludioxonil aqueous emulsion.
The lentinan fludioxonil water dispersible granules of example of formulations 4 11%
Component composition is following (each component is by weight percentage)：
Preparation method is：Above-mentioned each active component, lauryl sodium sulfate, wetting agent, disintegrant, filler are filled in proportion
Divide mixing, be crushed to 5 μm of particle diameter through airslide disintegrating mill, obtain female powder, be granulated after female powder and alkyl benzene sulfonate calcium salt are sufficiently mixed,
Drying, sieving, so as to which 11% lentinan fludioxonil water dispersible granules be made.
The lentinan fludioxonil microemulsion of example of formulations 5 18%
Component composition is following (each component is by weight percentage)：
Preparation method is：Above-mentioned each active component, various auxiliary agents are sufficiently mixed in proportion, make to be dissolved into homogeneous oil phase,
Under high-speed stirred, oil phase is mixed with water, obtains 18% lentinan fludioxonil microemulsion.
Application example 1：The gray mold test of pesticide effectiveness after chemical control grape harvest
In Tai'an Daiyue District wang jiazhuang village vineyard, kind was huge peak to August in 2016 on 30th, selection without rot, without shattering,
Fruit ear without machinery wound carries out soaking fruit experiment.Whole fruit ear is immersed into each chemicals treatment (20% mushroom prepared by embodiment 1 respectively
2000 times, 1500 times, 1000 times of polysaccharide fludioxonil suspending agent；50% 1500 times of fludioxonil wettable powder, 400 g/l it is phonetic
Mould 1200 times of amine suspending agent, 400 times of 50% carbendazol wettable powder) and clear water control in, the leaching fruit time is 30s, is then put
Dried in the shade in the sterilizing filter paper of the lower place mat of field tree.Then Shandong Fruit-tree Inst. is transported back, in fresh-keeping warehouse (temperature 0 ± 0.5
DEG C) in precooling 24h, then weigh 2.0kg/ bags and be carefully placed into PE freshness protection packages, plastics fruit packaging can be had enough to meet the need by being then neatly put into
In case, one bag is put per case, totally 108 case, is finally putting into fresh-keeping warehouse (temperature：0 ± 0.5 DEG C) in stored.The respectively processing point per 15d
3 casees fruits are not taken out, carry out rate investigation of going rotten, and the measure of the percentage of thresh, weight-loss ratio investigation and flesh firmness is carried out during 60d.Fruit
Determination of Hardness, test is compressed using TA.XT.Plus property testers P/75 probes, each processing takes 20 fruits at random.
Processing method is same as above under 25 DEG C of normal temperature.
Drug effect statistical analysis technique：Pharmacy control efficacy is calculated by following equation；It is poor that the new multipoles of Duncan are carried out using DPS softwares
Examine its otherness (P≤0.05).As a result it is as shown in table 2.20% lentinan fludioxonil suspending agent preventing and treating post-harvest grapes ash
Mildew the results are shown in Table 3.
a.
b.
c.
d.
Each index investigation result during the Grape During Storage of table 2
The pharmacodynamic results of gray mold after the lentinan fludioxonil suspending agent of table 3 20% preventing and treating grape harvest
From table 2, table 3, fruit processing is soaked after 20% 1500 times of lentinan fludioxonil suspending agent, 1000 times are adopted
Afterwards, every index of quality is investigated in Grape During Storage 60d outbounds, as rotting rate, the percentage of thresh, weight-loss ratio are significantly better than 50% fludioxonil
1500 times of wettable powder, 400 times of 50% carbendazol wettable powder, clear water control.Under refrigerated condition, before 60d, 20% is fragrant
For the processing of 1000 times of mushroom polysaccharide fludioxonil suspending agent to the preventive effect of grape grey mould always more than 80%, significant effect is better than it
He is respectively handled.Therefore,, can be notable using 20% lentinan fludioxonil suspending agent leaching fruit processing grape fruit ear after grape harvest
The generation of gray mold during suppression Grape During Storage, so as to extend storage period, improves storage commodity.
Biological Examples 2：18% lentinan fludioxonil microemulsion prevents and treats garlic stems storage period disease
On May 1st, 2016 is tested in Jinxiang County Yu Shan towns special freezer of peasant household's garlic stems.Before garlic stems harvesting storage, in
Embodiment 5 prepare 18% 1500 times of lentinan fludioxonil microemulsion, 1000 times, 25% 250 times of prochloraz aqueous emulsion, clearly
Dipping garlic stems tongue slightly 30s in water control, is put in storage storage after draining away the water.After garlic stems is cooled to -0.3 DEG C ± 0.2 DEG C in advance, load silicon
Window bag is stored, the normal freezer management together with other garlic stems.The experimental observation phase is 240 days.Each 4 repetitions of processing, Mei Gechong
Multiple 2 bags (every bag of about 19kg garlic stems).Respectively at 90d, 120d, 150d, 180d, 210d, 240d investigation garlic stems rotting rate, tongue slightly face
Each index such as color.As a result it is as shown in table 4.
a.
b.
The lentinan fludioxonil microemulsion of table 4 18% prevents and treats the pharmacodynamic results of garlic stems gray mold
As shown in Table 4, after slightly processing is soaked after 18% 1500 times of lentinan fludioxonil microemulsion, 1000 times are adopted, garlic
A kind of sedge stores 240d prevention effects more than 88%, and tongue slightly color is normal, no phenomenon of losing water, shows good anti-corrosive fresh-keeping
Effect, it is significantly better than 1500 times of 50% fludioxonil wettable powder, 250 times of 25% prochloraz aqueous emulsion.Therefore, after garlic stems is adopted,
Using 18% lentinan fludioxonil microemulsion dipping tongue slightly, the generation of gray mold in garlic stems storage can be significantly inhibited,
So as to extend storage period, storage commodity is improved.
Claims (10)
1. lentinan combines application of the fludioxonil in preventing and treating fruit, vegetable storage disease.
2. application according to claim 1, it is characterised in that：The fruit be selected from grape, cherry, blueberry, strawberry, peach,
Apple, pears；The vegetables are selected from tomato, cucumber, eggplant, leek, garlic stems.
3. application according to claim 1, it is characterised in that：The disease is the microbial disease of Botrytis cinerea, such as
Grape grey mould, garlic stems gray mold.
4. application according to claim 1, it is characterised in that：During concrete application, lentinan and fludioxonil compounding are made
Into bactericidal composition, after being diluted with water, sprinkling or dipping are in fruit, vegetable surface, after drying, freezer normal storage；Sterilization group
In compound, the weight ratio of lentinan and fludioxonil is 1~10:1~15, preferably 1:1~10；
Or：During concrete application, it will be sprayed respectively by the preparation of active component, the preparation using fludioxonil as active component of lentinan
Spill or dipping is in fruit, vegetable surface, after drying, freezer normal storage.
5. application according to claim 4, it is characterised in that：The bactericidal composition is the component group by following parts by weight
Into：Lentinan 0.5%~15%, fludioxonil 0.5%~50%, surplus are to allow to use and acceptable in Pesticide Science
Auxiliary material.
A kind of 6. antisepsis antistaling agent for water fruits and vegetables, it is characterised in that：It is made up of the component of following parts by weight：It is fragrant
Mushroom polysaccharide 0.5%~15%, fludioxonil 0.5%~50%, surplus are to allow to use and acceptable auxiliary material in Pesticide Science.
7. antisepsis antistaling agent according to claim 6, it is characterised in that：Allow to use and can receive in the Pesticide Science
Auxiliary material be selected from solvent, dispersant, diffusant, disintegrant, emulsifying agent, adsorbent, stabilizer, wetting agent, defoamer, antifreezing agent
Deng and other stabilizations for contributing to active ingredient drug effect and performance allow use auxiliary agent.
8. the antisepsis antistaling agent according to claim 6 or 7, it is characterised in that：The formulation of the antisepsis antistaling agent is selected from can
WP, suspending agent, aqueous emulsion, water dispersible granules, microemulsion；
When for suspending agent when, be made up of the component of following percetage by weight：
When for wettable powder when, be made up of the component of following percetage by weight：
When for aqueous emulsion when, be made up of the component of following percetage by weight：
When for water dispersible granules when, be made up of the component of following percetage by weight：
When for microemulsion when, be made up of the component of following percetage by weight：
A kind of 9. preservation method of water fruits and vegetables, it is characterised in that：Bactericidal composite is made in lentinan and fludioxonil compounding
Thing, after being diluted with water, sprinkling or dipping are in fruit, vegetable surface, after drying, freezer normal storage；In bactericidal composition, mushroom
The weight of polysaccharide and fludioxonil ratio is 1~10:1~15, preferably 1:1~10；
Or：Respectively will using lentinan as active component preparation, using fludioxonil as active component preparation sprinkling or dipping in
Fruit, vegetable surface, after drying, freezer normal storage.
10. the preservation method of water fruits and vegetables according to claim 9, it is characterised in that：The bactericidal composition be by with
The component composition of lower parts by weight：Lentinan 0.5%~15%, fludioxonil 0.5%~50%, surplus are to allow in Pesticide Science
Using with acceptable auxiliary material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710796782.8A CN107494710A (en) | 2017-09-06 | 2017-09-06 | Lentinan combines application of the fludioxonil in preventing and treating fruit, vegetable storage disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710796782.8A CN107494710A (en) | 2017-09-06 | 2017-09-06 | Lentinan combines application of the fludioxonil in preventing and treating fruit, vegetable storage disease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107494710A true CN107494710A (en) | 2017-12-22 |
Family
ID=60695577
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710796782.8A Pending CN107494710A (en) | 2017-09-06 | 2017-09-06 | Lentinan combines application of the fludioxonil in preventing and treating fruit, vegetable storage disease |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107494710A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115590037A (en) * | 2022-10-17 | 2023-01-13 | 姚永松（Cn） | Fruit and vegetable biological bactericide and preparation method and application thereof |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0741970A1 (en) * | 1993-12-02 | 1996-11-13 | Sumitomo Chemical Company Limited | Bactericidal composition |
| CN101642126A (en) * | 2009-07-09 | 2010-02-10 | 深圳诺普信农化股份有限公司 | Compound bactericidal composition containing fludioxonil |
| CN102037968A (en) * | 2011-01-17 | 2011-05-04 | 河北省农林科学院植物保护研究所 | Compound bactericide for mainly treating grey mould of fruits and vegetables |
| CN102972448A (en) * | 2012-12-25 | 2013-03-20 | 联保作物科技有限公司 | Disease-resistant composition, and application and suspended seed coating agent thereof |
| CN103168781A (en) * | 2013-03-26 | 2013-06-26 | 河北省农林科学院植物保护研究所 | Compound sterilizing agent containing fluopicolide and fludioxonil and application of sterilizing agent |
| CN103563958A (en) * | 2012-08-09 | 2014-02-12 | 南京华洲药业有限公司 | Bactericidal composition containing prochloraz and lentinan and application thereof |
| CN103636625A (en) * | 2013-12-20 | 2014-03-19 | 北京燕化永乐生物科技股份有限公司 | Bactericidal composition |
| CN103651364A (en) * | 2013-12-25 | 2014-03-26 | 山东农业大学 | Lentinan and fludioxonil-containing sterilization disease-resistant composition |
| CN104430833A (en) * | 2014-12-19 | 2015-03-25 | 山东营养源食品科技有限公司 | Fruit and vegetable fresh-keeping agent |
| CN105557993A (en) * | 2015-12-29 | 2016-05-11 | 河北省农林科学院植物保护研究所 | Application of fludioxonil and compound thereof in preventing and treating diseases of sweet potato during storage |
| CN106387033A (en) * | 2016-09-12 | 2017-02-15 | 广西南宁乐蕊生物科技有限责任公司 | Banana preservative containing sucrose ester and lentinan |
-
2017
- 2017-09-06 CN CN201710796782.8A patent/CN107494710A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0741970A1 (en) * | 1993-12-02 | 1996-11-13 | Sumitomo Chemical Company Limited | Bactericidal composition |
| CN101642126A (en) * | 2009-07-09 | 2010-02-10 | 深圳诺普信农化股份有限公司 | Compound bactericidal composition containing fludioxonil |
| CN102037968A (en) * | 2011-01-17 | 2011-05-04 | 河北省农林科学院植物保护研究所 | Compound bactericide for mainly treating grey mould of fruits and vegetables |
| CN103563958A (en) * | 2012-08-09 | 2014-02-12 | 南京华洲药业有限公司 | Bactericidal composition containing prochloraz and lentinan and application thereof |
| CN102972448A (en) * | 2012-12-25 | 2013-03-20 | 联保作物科技有限公司 | Disease-resistant composition, and application and suspended seed coating agent thereof |
| CN103168781A (en) * | 2013-03-26 | 2013-06-26 | 河北省农林科学院植物保护研究所 | Compound sterilizing agent containing fluopicolide and fludioxonil and application of sterilizing agent |
| CN103636625A (en) * | 2013-12-20 | 2014-03-19 | 北京燕化永乐生物科技股份有限公司 | Bactericidal composition |
| CN103651364A (en) * | 2013-12-25 | 2014-03-26 | 山东农业大学 | Lentinan and fludioxonil-containing sterilization disease-resistant composition |
| CN104430833A (en) * | 2014-12-19 | 2015-03-25 | 山东营养源食品科技有限公司 | Fruit and vegetable fresh-keeping agent |
| CN105557993A (en) * | 2015-12-29 | 2016-05-11 | 河北省农林科学院植物保护研究所 | Application of fludioxonil and compound thereof in preventing and treating diseases of sweet potato during storage |
| CN106387033A (en) * | 2016-09-12 | 2017-02-15 | 广西南宁乐蕊生物科技有限责任公司 | Banana preservative containing sucrose ester and lentinan |
Non-Patent Citations (1)
| Title |
|---|
| 张瑾等: "杏采后病害病原菌鉴定及室内药剂筛选", 《植物保护》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115590037A (en) * | 2022-10-17 | 2023-01-13 | 姚永松（Cn） | Fruit and vegetable biological bactericide and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104396970A (en) | Sterilizing composition containing zinc thiazole, preparation and applications thereof | |
| CN102763657A (en) | Germicide composite with synergistic effect | |
| CN101444236A (en) | Application of tea saponin and mixture thereof in antisepsis and preservation of fruit | |
| CN101361485B (en) | Compound bactericidal agent containing zincpolyanemine and ethylicin | |
| CN107927149A (en) | A kind of application of the p-Coumaric Acid ester in sterilizing anti-staling agent for fruit and vegetable and the sterilizing anti-staling agent for fruit and vegetable of the ester containing p-Coumaric Acid | |
| KR20180019194A (en) | An antimicrobial composition comprising a food-approved antimicrobial agent and zinc pyrithione | |
| CN105454273B (en) | A kind of microbicide compositions | |
| CN107668194A (en) | Fludioxonil combines application of the chitosan oligosaccharide in preventing and treating fruit, vegetable storage disease | |
| CN104012647A (en) | Loquat fruit preservation method through nano packaging compound plant essential oil treatment | |
| CN102119693B (en) | Synergetic antibacterial composition containing fluazinam and tebuconazole and use thereof | |
| Gupta et al. | Safety of fresh fruits and vegetables | |
| CN107494710A (en) | Lentinan combines application of the fludioxonil in preventing and treating fruit, vegetable storage disease | |
| CN110573017A (en) | formulation comprising at least fludioxonil and a mixture comprising a strain of aureobasidium pullulans | |
| CN101438703B (en) | Sterilizing compositional composition containing eugenol | |
| CN107668195A (en) | Fludioxonil combines application of the cinnamic acid in preventing and treating fruit, vegetable storage disease | |
| CN102302012A (en) | Pesticide composition | |
| CN102119691B (en) | Bactericidal composition containing fluazinam and difenoconazole and application of same | |
| CN109645112A (en) | A kind of bactericidal composition and its application of probenazole-containing and procymidone | |
| CN104782747B (en) | A kind of preservation method of mulberry fruit | |
| CN103858885B (en) | The bactericidal composition of control apple disease and application thereof | |
| CN106615064A (en) | Mango fresh-keeping agent with sucrose ester and cinnamaldehyde | |
| CN102119699B (en) | Bactericidal composition containing fluazinam and prochloraz and application thereof | |
| CN105432639A (en) | Sterilization composition for preventing and curing vegetable disease and application | |
| CN109730076A (en) | A kind of bactericidal composition and its application containing difenoconazole and pyrimethanil | |
| CN104798794A (en) | Captan and imazalil compound composition and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171222 |