CN115590037A - Fruit and vegetable biological bactericide and preparation method and application thereof - Google Patents
Fruit and vegetable biological bactericide and preparation method and application thereof Download PDFInfo
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- CN115590037A CN115590037A CN202211270878.8A CN202211270878A CN115590037A CN 115590037 A CN115590037 A CN 115590037A CN 202211270878 A CN202211270878 A CN 202211270878A CN 115590037 A CN115590037 A CN 115590037A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- Engineering & Computer Science (AREA)
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- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Microbiology (AREA)
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- Biotechnology (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of agricultural biological control, and particularly relates to a fruit and vegetable biological bactericide as well as a preparation method and application thereof. The fruit and vegetable biological bactericide comprises bacillus subtilis, lentinan, berberine hydrochloride and a solvent. The invention has the beneficial effects that: according to the fruit and vegetable biological bactericide provided by the invention, bacillus subtilis, lentinan and berberine hydrochloride are compounded for use, the bactericidal effect is synergistically improved, experimental research on test crops of Kyoho grapes shows that spraying is started at the early stage of grape gray mold attack, spraying is carried out once every 15 days, spraying is continuously carried out for 3 times, and the prevention and treatment effect is investigated after spraying is carried out for 16 days at the last time, so that the prevention and treatment effect of the fruit and vegetable biological bactericide on grape gray mold reaches 96.99%, and the prevention and treatment effect is obvious.
Description
Technical Field
The invention belongs to the technical field of agricultural biological control, and particularly relates to a fruit and vegetable biological bactericide as well as a preparation method and application thereof.
Background
Grape gray mold is commonly called as 'corncob rot', also called grape gray mold, and the pathogen is Botrytis cinerea (Botrytis cinerea Pers), which belongs to fungi of Deuteromycotina. A sexual generation is Staphylococcus aureus (Botryotina fuckliana (de Bary) Whetzel.). Grape gray mold is a major hazard to inflorescences, young fruits and mature fruits, and sometimes to young shoots, leaves and fruit stalks. The most of the flower ears are attacked before blooming, inflorescences are hot water in the beginning of damage, are dark brown, are soft and rotten in diseased tissues, are densely covered with grey mildew layers on the surfaces, are wilted by damaged inflorescences, and are easy to shed young fruits; the fruit stalks are black brown after being infected with diseases, and black blocky sclerotia is generated on disease spots sometimes; after grape blossoms, the clusters are in a light brown water immersion state at the beginning of disease infection, the clusters are quickly changed into dark brown, the whole clusters are soft and rotten, and a light gray mildew layer grows on the clusters after 2-3 days if the clusters meet rainy days; the fruits are infected in the near-mature period, and light brown concave disease spots are firstly generated to quickly spread the whole fruits so as to cause the fruits to rot. When the disease is serious, the whole ear or a part of the ear is rotten, a gray mildew layer of a mouse grows on the gray mildew layer, the stalk becomes black after the disease is ill, black blocky sclerotia grows at the later stage, the whole ear grows full of the mildew layer after the disease is finally expanded, and the fruit is completely rotten. The young leaves can also be infected with diseases to generate irregular brown spots, irregular ring lines sometimes appear on the spots, irregular gray mould layers are generated when the spots are wet, each leaf generally has 2-5 spots, and when the spots are serious, a gray mould layer of a rat can also grow on the diseased part, and finally the disease is dried up. Is one of the destructive diseases of grapes, and often causes the reduction of yield and the failure of harvest of farmers. The pathogen can infect grape, eggplant, tomato, cabbage, apple, kidney bean, pepper, cowpea, boll, onion, etc.
At present, chemical control, biological control, chemical and biological mixed control and the like are mostly adopted in the control technology for advanced control. Once the disease occurs, the control effect is not ideal, and the existing control medicament has resistance due to long-term use, so that the development of a new green and efficient control medicament is urgently needed.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a green and efficient biological control agent for fruit and vegetable diseases and insect pests, which has a remarkable effect of controlling grape gray mold.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a fruit and vegetable biological bactericide, which comprises bacillus subtilis, lentinan, berberine hydrochloride and a solvent.
Bacillus subtilis, 1, competition: the plant growth regulator can be rapidly and massively propagated and planted in plants, effectively prevents the propagation of pathogenic microorganisms, interferes the infection of the plant pathogenic microorganisms to the plants, and destroys the colonization of the pathogenic microorganisms on the plants, thereby achieving the effects of inhibiting bacteria and controlling diseases. 2. And (3) bacteriolysis: the bacillus subtilis can be adsorbed on hyphae of pathogenic fungi, and can generate a bacteriolytic substance in the growth process along with the joint growth of the hyphae, so that the mycelium is digested; typically resulting in hyphal rupture, disintegration or cytoplasmic digestion; or the secondary metabolites can dissolve the cell walls of pathogenic spores, resulting in cell wall perforation, malformation and the like. 3. Production of antibacterial substances: antibiotic substances are produced which inhibit the growth of bacteria, viruses, fungi and pathogens, these antibiotic substances being based on antibiotics such as phospholipids, aminosugars, peptides and lipopeptides. Among them, lipopeptide antibiotics are the most important antibacterial substances of bacillus subtilis.
Lentinan has disease-resistant and bacteriostatic activity, and can induce plants to generate and accumulate disease-resistant substances such as salicylic acid, phytoalexin and chitinase. The lentinan can induce crops to have a certain inhibition effect on plant pathogenic bacteria, can promote the germination and growth of the crops, and can improve the stress resistance of the crops.
Berberine hydrochloride, also known as berberine hydrochloride, of the formula [ C 20 H 18 NO 4 ] + The bactericide belongs to a broad-spectrum biological bactericide, has wider bactericidal action, higher bactericidal activity, double effects of protection and treatment, quick, high and lasting effect, has special effect on various fungal diseases of various crops, and mainly plays a role in inhibiting growth and reproduction by interfering the metabolism of pathogens through osmosis.
The invention combines the bacillus subtilis, the lentinan and the berberine hydrochloride to play a synergistic effect.
According to the fruit and vegetable biological bactericide of the invention, each 1000ml of the fruit and vegetable biological bactericide contains: bacillus subtilis 4.8X 10 3 ～7.2×10 3 Billion spores, 1.6-2 g lentinan and 1.6-2.4 g berberine hydrochloride. Preferably, the biological bactericide for fruits and vegetables contains per 1000 ml: bacillus subtilis 6X 10 3 Billion spores, lentinan 0.018g, berberine hydrochloride 0.02g. Preferably, the solvent is water.
The preparation method of the fruit and vegetable biological bactericide comprises the following specific steps: respectively adding Bacillus subtilis, lentinan and berberine hydrochloride into water, and mixing. Preferably, 1200-1800 mL of 100 hundred million spores/mL bacillus subtilis solution, 40-50 mL of 1g/mL lentinan solution and 80-120 mL of 0.5g/mL berberine hydrochloride solution are added into 25000mL of water and mixed uniformly to obtain the product.
The fruit and vegetable biological bactericide is expected to be used for preventing and treating other various fruit and vegetable diseases and insect pests besides grape gray mold, so the invention also relates to the application of the fruit and vegetable biological bactericide in preparing fruit and vegetable disease and insect pest biological prevention and treatment medicaments, in particular to grape gray mold prevention and treatment medicaments.
The invention has the beneficial effects that:
according to the fruit and vegetable biological bactericide provided by the invention, bacillus subtilis, lentinan and berberine hydrochloride are compounded for use, the bactericidal effect is synergistically improved, experimental research on test crops of Kyoho grapes shows that spraying is started at the early stage of grape gray mold attack, spraying is carried out once every 15 days, spraying is continuously carried out for 3 times, and the prevention and treatment effect is investigated after spraying is carried out for 16 days at the last time, so that the prevention and treatment effect of the fruit and vegetable biological bactericide on grape gray mold reaches 96.99%, and the prevention and treatment effect is obvious.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.
Example 1
A biological bactericide for fruits and vegetables comprises Bacillus subtilis, lentinan, berberine hydrochloride and water.
The preparation method comprises the following steps: adding 1500mL of 100 hundred million spores/mL bacillus subtilis solution, 45mL of 1g/mL lentinan solution and 100mL of 0.5g/mL berberine hydrochloride solution into 25000mL of water, and mixing uniformly to obtain the product.
Example 2
A biological bactericide for fruits and vegetables comprises Bacillus subtilis, lentinan, berberine hydrochloride and water.
The preparation method comprises the following steps: 1200mL of 100 hundred million spores/mL bacillus subtilis solution, 40mL of 1g/mL lentinan solution and 80mL of 0.5g/mL berberine hydrochloride solution are added into 25000mL of water and mixed uniformly to obtain the product.
Example 3
A biological bactericide for fruits and vegetables comprises Bacillus subtilis, lentinan, berberine hydrochloride and water.
The preparation method comprises the following steps: 1800mL of 100 hundred million spores/mL bacillus subtilis solution, 50mL of 1g/mL lentinan solution and 120mL of 0.5g/mL berberine hydrochloride solution are added into 25000mL of water and mixed uniformly to obtain the product.
Experimental study on prevention and treatment effects of grape gray mold
(1) Design of experiments
Treatment group 1: example 1 of the invention;
treatment group 2: 1500mL of 100 hundred million spores/mL bacillus subtilis solution is added into 25000mL of water;
treatment group 3: adding 45mL of lentinan solution of 1g/mL into 25000mL of water;
treatment group 4: adding 100mL of 0.5g/mL berberine hydrochloride solution into 25000mL of water;
treatment group 5: clear water Control (CK);
treatment group 6: adding 1500mL of 100 hundred million spores/mL of bacillus subtilis solution and 45mL of 1g/mL of lentinan solution into 25000mL of water;
treatment group 7: 1500mL of 100 hundred million spores/mL bacillus subtilis solution and 100mL of 0.5g/mL berberine hydrochloride solution are added into 25000mL of water;
treatment group 8: 45mL of lentinan solution with the concentration of 1g/mL and 100mL of berberine hydrochloride solution with the concentration of 0.5g/mL are added into 25000mL of water.
(2) Test method
The test site is in the mountain village town and the township village of stream pool, fuan city, ningde city, fujian province, and the grape planting base of farmer planting professional cooperative society is well reclaimed in spring, the crop to be tested is Kyoho grapes, the medicine spraying is started at the early stage of grape gray mold attack, the medicine spraying is carried out once every 15 days, the medicine spraying is carried out for 3 times continuously, and the control effect is investigated 16 days after the last medicine spraying. Test time: 2021, 3/18. The test was run with a total of 7 treatments, 1 clear water control, 3 replicates of each treatment, 8 cells, 21.3 square meters per cell.
(3) Investigation method
Sampling 5 points in each cell by adopting a large five-point method, investigating 1 plant at each point, randomly selecting 3 spica (fruit ears) on, in and under each plant, investigating the number of the spica (fruit ears) in each treated cell, and calculating the disease spica (fruit ear) rate, disease index and prevention and treatment effect;
diseased flower ear (ear) rate (%) = diseased flower ear (ear) number/total survey flower ear (ear) number × 100;
disease index = (number of diseased ears (ears) at each stage × disease grade value)/(number of total investigated ears (ears) × highest grade value) × 100;
control effect (%) = (blank control disease index-disease index after drug treatment area)/blank control disease index x 100;
the grading standard of grape gray mold is as follows:
level 0: no disease; level 1: the lesion area accounts for less than 5% of the area of the whole flower ear (fruit ear);
and 3, level: the lesion area accounts for 6 to 25 percent of the area of the whole flower spike (fruit spike);
and 5, stage: the lesion area accounts for 26-50% of the area of the whole flower ear (ear);
and 7, stage: the lesion area accounts for more than 51% of the area of the whole flower spike (fruit spike).
TABLE 1 control of grape Gray mold
As can be seen from Table 1, the treatment group 1, namely the control effect of the fruit and vegetable biological bactericide containing three components of Bacillus subtilis, lentinan and berberine hydrochloride on grape gray mold reaches 96.99%, which is obviously superior to the treatment groups 2 to 4, namely the control effect of the fruit and vegetable biological bactericide containing any one of the three components of Bacillus subtilis, lentinan and berberine hydrochloride on grape gray mold (33.82% -69.92%), and the treatment groups 6 to 8, namely the control effect of the fruit and vegetable biological bactericide containing any two of the three components of Bacillus subtilis, lentinan and berberine hydrochloride on grape gray mold (70.68% -76.69%), which shows that the fruit and vegetable biological bactericide containing the three components of Bacillus subtilis, lentinan and berberine hydrochloride has a synergistic effect and remarkable control effect. In addition, phytotoxicity investigation showed that treatment group 1 had no phytotoxicity effect on target and non-target crops by visual inspection for 15 consecutive days after the first application, 15 consecutive days after the second application, and 16 consecutive days after the third application.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (8)
1. The fruit and vegetable biological bactericide is characterized by comprising bacillus subtilis, lentinan, berberine hydrochloride and a solvent.
2. The fruit and vegetable biological bactericide according to claim 1, which isIs characterized in that each 1000ml of the fruit and vegetable biological bactericide contains: bacillus subtilis 4.8X 10 3 ～7.2×10 3 Billion spores, 1.6-2 g lentinan and 1.6-2.4 g berberine hydrochloride.
3. The biological bactericide for fruits and vegetables according to claim 1, wherein each 1000ml of the biological bactericide for fruits and vegetables comprises: bacillus subtilis 6X 10 3 Billion spores, lentinan 0.018g, berberine hydrochloride 0.02g.
4. The fruit and vegetable biological bactericide according to any one of claims 1 to 3, wherein the solvent is water.
5. The preparation method of the fruit and vegetable biological bactericide of claim 4, which is characterized by comprising the following steps: respectively adding Bacillus subtilis, lentinan and berberine hydrochloride into water, and mixing.
6. The preparation method of the fruit and vegetable biological bactericide as claimed in claim 4, which is characterized by comprising the following steps: adding 1200-1800 mL of 100 hundred million spores/mL bacillus subtilis solution, 40-50 mL of 1g/mL lentinan solution and 80-120 mL of 0.5g/mL berberine hydrochloride solution into 25000mL of water, and uniformly mixing to obtain the product.
7. The use of the fruit and vegetable biological bactericide according to any one of claims 1 to 3 for preparing a biological control medicament for fruit and vegetable diseases and insect pests.
8. The use of claim 7, wherein the fruit and vegetable pest is grape gray mold.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211270878.8A CN115590037A (en) | 2022-10-17 | 2022-10-17 | Fruit and vegetable biological bactericide and preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211270878.8A CN115590037A (en) | 2022-10-17 | 2022-10-17 | Fruit and vegetable biological bactericide and preparation method and application thereof |
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| CN115590037A true CN115590037A (en) | 2023-01-13 |
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| CN202211270878.8A Pending CN115590037A (en) | 2022-10-17 | 2022-10-17 | Fruit and vegetable biological bactericide and preparation method and application thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102876605A (en) * | 2012-09-23 | 2013-01-16 | 江苏丘陵地区镇江农业科学研究所 | Bacillus subtilis DJ-6 and application thereof to prevention and treatment of grape disease |
| CN105907680A (en) * | 2016-05-27 | 2016-08-31 | 河北省科学院生物研究所 | Bacillus subtilis J-5 as well as biological agent and application thereof |
| CN107494710A (en) * | 2017-09-06 | 2017-12-22 | 山东省果树研究所 | Lentinan combines application of the fludioxonil in preventing and treating fruit, vegetable storage disease |
| CN108142439A (en) * | 2016-12-06 | 2018-06-12 | 黄永 | A kind of life assemblage preparation of plant pest management |
-
2022
- 2022-10-17 CN CN202211270878.8A patent/CN115590037A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102876605A (en) * | 2012-09-23 | 2013-01-16 | 江苏丘陵地区镇江农业科学研究所 | Bacillus subtilis DJ-6 and application thereof to prevention and treatment of grape disease |
| CN105907680A (en) * | 2016-05-27 | 2016-08-31 | 河北省科学院生物研究所 | Bacillus subtilis J-5 as well as biological agent and application thereof |
| CN108142439A (en) * | 2016-12-06 | 2018-06-12 | 黄永 | A kind of life assemblage preparation of plant pest management |
| CN107494710A (en) * | 2017-09-06 | 2017-12-22 | 山东省果树研究所 | Lentinan combines application of the fludioxonil in preventing and treating fruit, vegetable storage disease |
Non-Patent Citations (1)
| Title |
|---|
| 孙华等: "接骨木提取物有效成分含量与抑菌活性的关系", 《聊城大学学报（自然科学版）》, vol. 24, no. 3, pages 39 - 42 * |
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