CN105505843A - Photosynthetic bacterium strain, liquid manure containing same, preparation method of liquid manure and application of liquid manure - Google Patents
Photosynthetic bacterium strain, liquid manure containing same, preparation method of liquid manure and application of liquid manure Download PDFInfo
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- CN105505843A CN105505843A CN201610105754.2A CN201610105754A CN105505843A CN 105505843 A CN105505843 A CN 105505843A CN 201610105754 A CN201610105754 A CN 201610105754A CN 105505843 A CN105505843 A CN 105505843A
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- 239000007788 liquid Substances 0.000 title claims abstract description 197
- 241000894006 Bacteria Species 0.000 title claims abstract description 49
- 230000000243 photosynthetic effect Effects 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims description 11
- 210000003608 fece Anatomy 0.000 title abstract description 12
- 239000010871 livestock manure Substances 0.000 title abstract description 11
- 230000000813 microbial effect Effects 0.000 claims abstract description 81
- 239000002994 raw material Substances 0.000 claims abstract description 21
- 238000004321 preservation Methods 0.000 claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 13
- 238000005286 illumination Methods 0.000 claims abstract description 13
- 230000004151 fermentation Effects 0.000 claims abstract description 12
- 239000003337 fertilizer Substances 0.000 claims description 73
- 241000190932 Rhodopseudomonas Species 0.000 claims description 35
- 239000000463 material Substances 0.000 claims description 23
- 230000001681 protective effect Effects 0.000 claims description 19
- 239000007921 spray Substances 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 238000003860 storage Methods 0.000 claims description 8
- 239000002054 inoculum Substances 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 5
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 238000009629 microbiological culture Methods 0.000 claims description 2
- 238000011161 development Methods 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 5
- 244000005700 microbiome Species 0.000 abstract description 5
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- 241000190946 Rhodopseudomonas sp. Species 0.000 abstract 3
- 238000012258 culturing Methods 0.000 abstract 1
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- 240000008067 Cucumis sativus Species 0.000 description 13
- 230000001580 bacterial effect Effects 0.000 description 13
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- 238000004519 manufacturing process Methods 0.000 description 9
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- 238000002474 experimental method Methods 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 244000144972 livestock Species 0.000 description 4
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- 235000005822 corn Nutrition 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241001057636 Dracaena deremensis Species 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 238000003973 irrigation Methods 0.000 description 2
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- 238000005259 measurement Methods 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
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- 241000251468 Actinopterygii Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 235000001018 Hibiscus sabdariffa Nutrition 0.000 description 1
- 239000004594 Masterbatch (MB) Substances 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 235000005291 Rumex acetosa Nutrition 0.000 description 1
- 240000007001 Rumex acetosella Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
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- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
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- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
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- 230000035558 fertility Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 239000004021 humic acid Substances 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000019633 pungent taste Nutrition 0.000 description 1
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- 239000013049 sediment Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
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- 235000003513 sheep sorrel Nutrition 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- -1 small molecules amino acid Chemical class 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
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- 239000013589 supplement Substances 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
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- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F7/00—Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses
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- Chemical & Material Sciences (AREA)
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- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Environmental & Geological Engineering (AREA)
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Abstract
The invention relates to a photosynthetic bacterium strain and liquid manure rich in photosynthetic bacterium strain and belongs to the technical fields of microbial fermentation, agricultural planting and the like. The photosynthetic bacterium strain is identified as Rhodopseudomonas sp. HL with the preservation number CGMCC No.11874 and can reproduce and grow rapidly by a biogas slurry matrix under an illumination or dark condition. The invention further provides application of the photosynthetic bacterium strain as the microorganism liquid manure. According to the application, the liquid manure is obtained by culturing the Rhodopseudomonas sp. HL in fresh biogas slurry serving as a raw material, and Rhodopseudomonas sp. HL concentration in bacterium liquid is not less than 108CFU/mL. The photosynthetic bacterium strain, the liquid manure rich in the photosynthetic bacterium strain and the application of the photosynthetic bacterium strain have the advantages that biogas slurry use and photosynthetic bacterium culture are coupled, so that the microbial liquid manure with multiple special properties is produced, and a cheap green biogas slurry high-added-value development technology and a cheap green biogas slurry high-added-value product are provided.
Description
Technical field
The present invention relates to a kind of microorganism strains and be rich in the Liquid Fertilizer of this bacterial strain, be specifically related to that a kind of microbial liquid based on natural pond liquid raw material is fertile, its preparation method and application thereof, belong to the technical field such as fermentable and agricultural planting.
Background technology
Along with the development of China's livestock and poultry breeding industry, the generation of annual livestock and poultry cultivation ight soil has reached 2,500,000,000 tons, and meanwhile, China is large agricultural country, and annual agriculture production produces more than 700,000,000 ton of agricultural crop straw.One that to take biogas engineering as the anaerobic fermentation technology of representative be can dissolve the organic wastes such as feces of livestock and poultry, agricultural crop straw, domestic refuse, alleviate the environmentally friendly technology of energy starved, obtain increasingly extensive application.According to statistics, by 2013, all kinds of biogas engineering of China more than 100,000 places, production and use of marsh gas user methane-generating pit 4,300 ten thousand family, and according to " planning of renewable energy source Long-and Medium-term Development ", to the year two thousand twenty, build up large-scale livestock and poultry farm biogas engineering 10000.With the extensive development of biogas engineering, significantly increasing of the fermentation residues such as its liquid natural pond, natural pond slag produced, annual natural pond, whole nation liquid quantity discharged is more than 1.3 hundred million tons, and the nutritive substances such as the organism containing high density in the liquid of natural pond and nitrogen, phosphorus, potassium, directly discharge causes very big pollution to environment.Therefore, how natural pond liquid dissolves has become the bottleneck problem of restriction biogas engineering development.At present recycling and qualified discharge process two aspect be can be summarized as to the method for natural pond liquid process, natural pond liquid recycling comprise natural pond liquid Fertilizer Transformed also field, natural pond immersion kind, natural pond liquid biological pesticide, natural pond liquid as substratum etc.; Liquid process up to standard in natural pond has high cost industrial treatment and artificial swamp or oxidation pond natural ecological treatment.Natural pond liquid is considered as the sewage of high organism, high nitrogen phosphorus by qualified discharge process, adopt the method such as biological, chemical to process, not utilizing or few nutritive substance utilized wherein, is a kind of simple pollutant abatement technology, processing cost is very high, is difficult to adopt in most of medium and small biogas engineering; Recycling is emphasized, to the utilization of natural pond liquid Middle nutrition material, to be developed by natural pond liquid as resource, is the optimal way disposing natural pond liquid.At present, the many low sides in field of agricultural cultivation of recycling of natural pond liquid are utilized as master, but along with the deep propelling of China's pesticide reduction pest controlling, Reducing amount of chemical fertilizer applied synergy action, the market demand potential of organic fertilizer and biological pesticide is huge, containing each amino acid, Plant hormones regulators,gibberellins, growth hormone, carbohydrate, nucleic acid and microbiotic etc. in the liquid of natural pond, be a kind of natural fertilizer and biological pesticide.Therefore, high added value exploitation is carried out to natural pond liquid, by significant to cracking the dissolve bottleneck problem of difficulty restriction China biogas engineering Sustainable development of natural pond liquid.At present, the natural pond additional exploitation of liquid height also have to be reported successively, as CN105254367A discloses one " a kind of natural pond liquid produces the method for drip irrigation special fertilizer ", the method utilizes the natural pond liquid clarified to be base-material, interpolation nutritive element and microbiobacterial agent are mixed with and obtain drip irrigation special fertilizer, but the method facility investment is large, and treatment operating costs is high.CN102503660A discloses one " High-efficiency microbial liquid fertilizer utilizing natural pond liquid to make and an application ", this bacterial manure is the natural pond liquid clarified and microbial function bacterium, plant-growth regulator, diffusant is masterbatch, be mixed with by a certain percentage and obtain microbial liquid bacterial manure, but with the addition of a large amount of Inorganic chemical composition in this bacterial manure, destruction is created to natural pond liquid natural components.
In sum, liquid high added value exploitation in natural pond is mostly stopped to the technological layers such as natural pond liquid is concentrated, natural pond liquid mixture, these technology or to there is facility investment huge, running cost is high; There is problems such as destroying natural pond liquid natural components.Therefore a kind of cheap green natural pond liquid high added value development technique of exploitation and product is needed badly.
Summary of the invention
The present invention is directed to the fertile Problems existing of existing microbial liquid, provide a plant height effect Photosynthetic bacterium strain and the microbial liquid fertilizer being rich in this Photosynthetic bacterium strain based on natural pond liquid raw material, the present invention also provides a kind of preparation method of this microbial liquid fertilizer.In addition, present invention also offers the application of this microbial liquid fertilizer.
Technical scheme of the present invention is, a strain Photosynthetic bacterium strain, and through being accredited as rhodopseudomonas (Rhodopseudomonassp.) HLCGMCCNo.11874, this bacterial strain can utilize natural pond liquid matrix Fast-propagation growth under illumination or dark condition.
This bacterial strain screens and obtains from sediment of pond sample, be preserved in China Microbial Culture Preservation Commission's common micro-organisms center, it is referred to as CGMCC, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101; Deposit number is: CGMCCNo.11874, and preservation date is: on December 14th, 2015.This bacterium properties and characteristics is as follows:
(1) morphological specificity
Thalline is shaft-like, slightly curved, thalline size 0.7 ~ 0.9 × 1.2 ~ 2.3 μm, and on solid plate, bacterium colony is round and little, in scarlet to dark red.
(2) cultural property
In liquid medium within anaerobism illumination or micro-aerobic illumination, cultivate after 3 days for 30 ~ 40 DEG C, bacterium liquid all takes on a red color to bright red, and the later stage transfers sorrel to, and old culture forms the precipitation that homogeneous easily spreads; At solid medium slat chain conveyor: cultivating for 30 ~ 40 DEG C 5 days can raised growth, bacterium colony rat, regular edges, little and justify, smooth corrugationless, bacterium colony is scarlet.
Present invention also offers a kind of microbial liquid fertile, described Liquid Fertilizer comprises the bacterium liquid of above-mentioned rhodopseudomonas (Rhodopseudomonassp.) HLCGMCCNo.11874, rhodopseudomonas HL concentration>=10 in bacterium liquid
8cFU/mL, described bacterium liquid for raw material with fresh natural pond liquid, is cultivated rhodopseudomonas HL and obtains.
Can also comprise protective material in described microbial liquid fertilizer, described protective material accounts for 1% ~ 5% of Liquid Fertilizer total mass.The bacterium liquid of described rhodopseudomonas HL for raw material with natural pond liquid, is cultivated rhodopseudomonas HL and obtains.Preferably, described protective material is one or both of glycerine or whiteruss.
Natural pond liquid is any residual aqueous phase thing after biogas engineering anaerobically fermenting, but photosynthetic bacterium is difficult to cultivate in the liquid of natural pond, and the rhodopseudomonas HL that the present invention filters out can utilize natural pond liquid fast culture well, so the invention provides a kind of preparation method of microbial liquid fertilizer, liquid matrix utilization in natural pond is cultivated with photosynthetic bacterium and is coupled, produce the microbial liquid fertilizer possessing multiple property.The method comprises the following steps:
(1) natural pond liquid charging: fresh natural pond liquid is loaded in rhodopseudomonas HL culture apparatus;
(2) rhodopseudomonas HL is inoculated: by rhodopseudomonas HL by 10
4~ 10
6the inoculum density of CFU/mL is inoculated in the culture apparatus of fresh natural pond liquid;
(3) stir oxygen of holding concurrently to cultivate: during fermentation, control leavening temperature, intensity of illumination and fermentation time; It stirs selects low speed paddle agitator, rotating speed 30r/min ~ 100r/min, and leavening temperature is 25 DEG C ~ 37 DEG C, and fermentation time is 3 days ~ 6 days, cultivates under illumination, and intensity of illumination is 2000Lx ~ 5000Lx;
(4) cultivation terminates rear storage: be rich in the fertile preservation under normal temperature or refrigerating temperature of rhodopseudomonas HL microbial liquid by what obtain after cultivation.
Preferably, described fresh natural pond liquid is taken from the natural pond liquid of stable aerogenesis biogas engineering fermentor tank discharge in more than 8 months.
What obtain after fermentation is rich in the preservation at normal temperatures of rhodopseudomonas HL microbial liquid fertilizer, and its validity period is 5 ~ 8 months; Or preservation under 4 DEG C ~ 8 DEG C refrigerating temperatures, its validity period is 12 ~ 14 months.
In order to extend the period of storage of microbial liquid fertilizer, the protective material of 1% ~ 5% mass ratio can be added wherein.Preferably, protective material can one in glycerine, whiteruss etc.When adding protective material, first protective material is added in a small amount of microbial liquid fertilizer, after stirring, add remaining microbial liquid fertilizer, and fully mix, make and with the addition of protectant microbial liquid fertilizer.
Present invention also offers mentioned microorganism Liquid Fertilizer to execute and the application on foliage-spray in roots of plants.
According to the requirement of the People's Republic of China's " composite microbiological fertilizer " agricultural industry criteria (NY/T789-2004) and detection method, the microbial liquid fertilizer product to preparation is tested, and the technical indicator of standard-required qualified product is: living bacteria count>=0.5 × 10
8cFU/mL, total nutrient (N+P
2o
5+ K
2o)>=4%, miscellaneous bacteria rate≤15%, pH value is 3-8, validity period>=3 month; The innoxious index of standard-required qualified product is: excrement colibacillus group number≤100/mL, induced worm egg death rate>=95%, As≤75mg/L, Cd≤10mg/L, Pb≤100mg/L, Cr≤150mg/L, Hg≤10mg/L.Prove through a large amount of tests, mentioned microorganism Liquid Fertilizer can reach following table technical indicator and innoxious index.
The technical indicator of microbial liquid fertilizer
The innoxious index of microbial liquid fertilizer
Superior part of the present invention is:
(1) a kind of microbial liquid based on natural pond liquid raw material provided by the invention is fertile, combine natural pond liquid and the two advantage of photosynthetic bacterium (rhodopseudomonas), not only can be crop and nutritive element is provided, also can supplement various crop benefit materials, as small molecules amino acid, VITAMIN, humic acids and various micro-isoreactivity material, also the soil organism can be improved.The photosynthetic bacterium of being simultaneously rich in can fixed nitrogen, solid carbon and the multiple toxic substance such as oxygenolysis sulfide and amine substance, promotes the growth of vegetables, paddy rice, seeding corn and other crops seedling, improves vegetables, the output of paddy rice and seeding corn and other crops and resistance against diseases.Meanwhile, the photosynthetic bacterium of being rich in can also improve soil microflora, promotes soil fertility.
(2) the present invention adopts the natural pond liquid of biogas engineering to be the microbial liquid fertilizer that photosynthetic bacterium is rich in culture medium raw material preparation, can by the whole recycling of natural pond liquid in large-sized biogas engineering, improve commodity and the added value of natural pond liquid, solve natural pond liquid to dissolve difficult problem, on the other hand for a kind of fertilizer of the microbial liquid based on natural pond liquid raw material of the present invention provides very cheap and sufficient raw material, there is obvious production cost advantage.
(3) embodiment of the fertile preparation method of microbial liquid of the present invention is simple, can be mass-produced, and is also easy to radiation and promotes.
(4) the present invention not only solves traditional natural pond liquid for the low drawback of fertilizer efficiency in agriculture production, simultaneously by fixed nitrogen and the sulfur fixation of photosynthetic bacterium, eliminate the pungency stenches such as the hydrogen sulfide in the liquid of natural pond, ammonia, achieve resource utilization safe handling and the utilization of natural pond liquid.
(5) the present invention is that root is filled with and executed the dual-purpose microbial liquid fertilizer with foliage-spray, can foliage-spray, can fill with again with water droplet, have that fertilising is easy, fertilizer in time, crop is easy to absorb, utilization rate of fertilizer advantages of higher.
Embodiment
Embodiment 1
(1) selection of rhodopseudomonas bacterial strain HL, step is as follows:
1) bed mud 10g being taken from fish pond, Zhen Xinjie village, Yongan, double fluid district, Sichuan grinds to be suspended from 90mL sterile distilled water and shakes evenly, boil 15min, gradient dilution after cooling, and coat in screening culture medium, 72h is cultivated at 30 DEG C, obtain bacterium colony, screening culture medium is consisting of of photosynthetic bacterium solid medium (g/L): yeast extract paste 1g, MgCl
20.2g, NH
4cl1g, K
2hPO
40.5g, NaCl1g, NaHCO
31g, moisturizing, to 1000mL, regulate pH to 6.8.
2) choosing colony scarlet, grow the strain of vigorous, ganoid bacterium colony 6, under identical culture condition, this 6 strain bacterium is carried out repeatedly streak culture more than three times, to obtain pure bacterial strain, be inoculated in photosynthetic bacterium liquid substratum, at 30 DEG C, cultivate 72h, obtain pure bacterial strain seed liquor.
3) by pure bacterial strain seed liquor according to 1 ~ 5% inoculum size be inoculated in the 500ml serum bottle containing 100ml liquid fermentation medium, in 30 DEG C, 100r/min fermentation culture 72h.Select No. 2 bacterium that fermented liquid is scarlet, cell concentration is higher, called after HL.
(2) physiological and biochemical property of rhodopseudomonas bacterial strain HL sees the following form 1.
The physiological and biochemical property of table 1 bacterial strain HL
Embodiment 2
A kind of weight percent of each component of the microbial liquid fertilizer based on natural pond liquid raw material is: the bacterium liquid 95% being rich in rhodopseudomonas HL, the glycerine protective material 5% cultivated for raw material with natural pond liquid.Finished product fertilizer all technical and innoxious index are as following table 2 and table 3.
The technical indicator of table 2 microbial liquid fertilizer
The innoxious index of table 3 microbial liquid fertilizer
Embodiment 3
A kind of weight percent of each component of the microbial liquid fertilizer based on natural pond liquid raw material is: the bacterium liquid 97% being rich in rhodopseudomonas HL, the whiteruss protective material 3% cultivated for raw material with natural pond liquid.Finished product fertilizer all technical and innoxious index are as following table 4 and table 5.
The technical indicator of table 4 microbial liquid fertilizer
The innoxious index of table 5 microbial liquid fertilizer
Embodiment 4
A kind of weight percent of each component of the microbial liquid fertilizer based on natural pond liquid raw material is: the bacterium liquid 99% being rich in rhodopseudomonas HL, the glycerine protective material 1% cultivated for raw material with natural pond liquid.Finished product fertilizer all technical and innoxious index are as following table 6 and table 7.
The technical indicator of table 6 microbial liquid fertilizer
The innoxious index of table 7 microbial liquid fertilizer
Embodiment 5
A kind of weight percent of component of the microbial liquid fertilizer based on natural pond liquid raw material is: the bacterium liquid 100% being rich in rhodopseudomonas HL cultivated for raw material with natural pond liquid.Finished product fertilizer all technical and innoxious index are as following table 8 and table 9.
The technical indicator of table 8 microbial liquid fertilizer
The innoxious index of table 9 microbial liquid fertilizer
Embodiment 6
Based on a preparation method for the microbial liquid fertilizer of natural pond liquid raw material, step is:
(1) fresh natural pond liquid charging: fresh natural pond liquid is loaded in rhodopseudomonas HL culture apparatus, fresh natural pond liquid is taken from the natural pond liquid of the biogas engineering fermentor tank discharge of Zhen Xinjie village, Yongan, Shuangliu County methane station, stablizes aerogenesis more than 8 months.Main component is as following table:
(2) rhodopseudomonas HL is inoculated: by rhodopseudomonas HL by 10
5the inoculum density of CFU/mL is inoculated in the liquid of fresh natural pond.
(3) stir oxygen of holding concurrently to cultivate: stir and select low speed paddle agitator, rotating speed 30r/min, leavening temperature is 30 DEG C, and ferment 4 days, cultivate under illumination, intensity of illumination is 3000Lx.
(4) cultivation terminates rear storage: obtain after cultivation being rich in the preservation at normal temperatures of rhodopseudomonas HL microbial liquid fertilizer, validity period is 6 months.
Embodiment 7
As different from Example 6: inoculum density 10
4cFU/mL; Rotating speed 100r/min, culture temperature is 25 DEG C, and fermentation time is 6 days, intensity of illumination 4000Lx; Cultivation obtains being rich in the fertile preservation under 4 DEG C of refrigerating temperatures of rhodopseudomonas HL microbial liquid, and its validity period is 13 months.
Embodiment 8
As different from Example 6: inoculum density 10
6cFU/mL; Rotating speed 70r/min, culture temperature is 35 DEG C, and fermentation time is 2 days, intensity of illumination 5000Lx; Cultivation obtains being rich in preservation under the fertile normal temperature of rhodopseudomonas HL microbial liquid, and its validity period is 8 months.
Embodiment 9
As different from Example 6: rotating speed 50r/min, culture temperature is 37 DEG C, and fermentation time is 1 day, preservation at normal temperatures, and its validity period is 5 months.
Embodiment 10
In order to extend the period of storage of microbial liquid fertilizer, in the microbial liquid fertilizer that embodiment 6 prepares, first add the glycerine of 2% mass ratio as protective material to a small amount of liquid fertilizer, preservation at normal temperatures, its validity period is 10 months.
Embodiment 11
In order to extend the period of storage of microbial liquid fertilizer, in the microbial liquid fertilizer that embodiment 7 prepares, first add the whiteruss protective material of 5% mass ratio to a small amount of liquid fertilizer, preservation at normal temperatures, its validity period is 11 months.
Embodiment 12
In order to extend the period of storage of microbial liquid fertilizer, in the microbial liquid fertilizer that embodiment 8 prepares, first add the whiteruss protective material of 3% mass ratio to a small amount of liquid fertilizer, preservation under refrigerating temperature at 4 DEG C, its validity period is 16 months.
Embodiment 13
In order to extend the period of storage of microbial liquid fertilizer, in the microbial liquid fertilizer that embodiment 9 prepares, first add the glycerine protective material of 2% mass ratio to a small amount of liquid fertilizer, preservation under refrigerating temperature at 4 DEG C, its validity period is 15 months.
Embodiment 14
Fertile potted plant of a kind of microbial liquid based on natural pond liquid raw material executes effect experimental: testing potted plant used is corn, first through hole-ground film, maize seedling is put into hole, then by the hole on film, fill with around every strain maize seedling rhizosphere and execute the fertile or common natural pond liquid of microbial liquid in 50mL embodiment 2, then water 250ml normal root water.
Setup Experiments three treatment group, are respectively:
(1) control group, does not use any fertilizer;
(2) common natural pond liquid group, uses common natural pond liquid;
(3) the fertile group of microbial liquid, the microbial liquid using embodiment 2 is fertile.
After maize seedling transplants 30 days, measure 0 ~ 10cm rhizosphere soil nitrogen fixation activity immediately below corn plants and and the fresh weight of corn plants.Measurement result is as shown in table 10 below, uses natural pond liquid and the fertile fresh weight that can both increase rhizosphere nitrogen fixation activity and milpa significantly of microbial liquid.Relative to control treatment group, common natural pond liquid treatment group rhizosphere nitrogen fixation activity and plant fresh weight add 9.5% and 27% respectively, and the fertile treatment group rhizosphere nitrogen fixation activity of microbial liquid and plant fresh weight add 23.3% and 55.8% respectively.Therefore, microbial liquid fertilizer can increase significantly maize seedling strain rhizosphere soil nitrogen fixation activity and seedling stage plant fresh weight.
The fertile potted plant experiment result of table 10 microbial liquid
| Testing index | Control group | Common natural pond liquid group | The fertile group of microbial liquid |
| Rhizosphere nitrogen fixation activity (μm .C 2H 4/ g. soil. sky) | 2.32 | 2.54 | 2.86 |
| Milpa fresh weight (g/ basin) | 81.2 | 103.1 | 126.5 |
Embodiment 15
The fertile potted plant foliage-spray effect experimental of microbial liquid based on natural pond liquid raw material: testing potted plant used is a cucumber, after cucumber seedling grows, the fertile or common natural pond liquid of the microbial liquid that every day sprays in the embodiment 3 through diluting 20 times to seedling.
Setup Experiments three treatment group, are respectively:
(1) control group, sprays clear water;
(2) common natural pond liquid group, sprays common natural pond liquid;
(3) the fertile group of microbial liquid, the microbial liquid spraying embodiment 3 is fertile.
According to the growth cycle of cucumber, measure cucumber plant height and every strain cucumber ultimate production respectively.Measurement result is as shown in table 11 below, and using natural pond liquid and microbial liquid fertilizer can both cucumber plant height and every strain cucumber ultimate production significantly.Relative to control treatment group, common natural pond liquid treatment group cucumber plant height and every strain cucumber ultimate production add 8.6% and 34.4% respectively, and the fertile treatment group cucumber plant height of microbial liquid and every strain cucumber ultimate production add 17.9% and 75% respectively.Therefore, microbial liquid fertilizer can increase cucumber plant height and cucumber ultimate production significantly.
The fertile potted plant experiment result of table 11 microbial liquid
Claims (6)
1. a strain Photosynthetic bacterium strain, it is characterized in that: code name is HL, through being accredited as rhodopseudomonas (Rhodopseudomonassp.), be preserved in China Microbial Culture Preservation Commission's common micro-organisms center on December 14th, 2015, preserving number is: CGMCCNo.11874; Bacterium colony is scarlet, and thalline is shaft-like, slightly curved, thalline size 0.7 ~ 0.9 × 1.2 ~ 2.3 μm, and on solid plate, bacterium colony is round and little, in scarlet to dark red.
2. a rhodopseudomonas HL microbial liquid is fertile, it is characterized in that: this microbial liquid fertilizer comprises the bacterium liquid of rhodopseudomonas HLCGMCCNo.11874, rhodopseudomonas HL concentration>=10 in bacterium liquid
8cFU/mL, described bacterium liquid for raw material with fresh natural pond liquid, is cultivated rhodopseudomonas HL and obtains.
3. a kind of rhodopseudomonas HL microbial liquid as claimed in claim 2 is fertile, it is characterized in that: this microbial liquid fertilizer also comprises protective material, and described protective material accounts for 1% ~ 5% of the fertile total mass of microbial liquid.
4. the preparation method of a kind of microbial liquid fertilizer as claimed in claim 2, is characterized in that, comprise the following steps:
(1) natural pond liquid charging: fresh natural pond liquid is loaded in rhodopseudomonas HL culture apparatus;
(2) rhodopseudomonas HL is inoculated: by rhodopseudomonas HL by 10
4~ 10
6the inoculum density of CFU/mL is inoculated in the culture apparatus of fresh natural pond liquid;
(3) stir oxygen of holding concurrently to cultivate: low speed paddle agitator speed 30r/min ~ 100r/min stirs, and leavening temperature is 25 DEG C ~ 37 DEG C, and fermentation time is 3 days ~ 6 days, cultivates under illumination, and intensity of illumination is 2000Lx ~ 5000Lx;
(4) cultivation terminates rear storage: be rich in the fertile preservation under normal temperature or refrigerating temperature of rhodopseudomonas HL microbial liquid by what obtain after cultivation.
5. the preparation method of a kind of microbial liquid fertilizer as claimed in claim 4, it is characterized in that, also comprise after step (3) with protective material step: protective material is joined in the microbial liquid fertilizer that a small amount of step (3) obtains, after stirring, add remaining microbial liquid fertile, and fully mix; Protectant add-on is 1% ~ 5% of the fertile quality of microbial liquid after adding protective material.
6. a kind of microbial liquid fertilizer as claimed in claim 2 is executed and the application on foliage-spray in roots of plants.
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| CN109867564A (en) * | 2019-01-31 | 2019-06-11 | 盐城师范学院 | A method of it eliminating biogas slurry agricultural biological fertilizer flexible package flatulence and improves fertilizer efficiency |
| CN110183260A (en) * | 2019-04-26 | 2019-08-30 | 河南农业大学 | A kind of plant nutrition liquid and its application using fermented liquid for biologically producing hydrogen preparation |
| CN112625956A (en) * | 2020-12-25 | 2021-04-09 | 江苏苏港和顺生物科技有限公司 | Method for culturing photosynthetic bacteria by utilizing membrane concentrated biogas slurry |
| CN113845383A (en) * | 2021-10-29 | 2021-12-28 | 安徽工程大学 | Biogas slurry fertilizer and production method and application thereof |
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