CN107840706A - A kind of microbial manure and its application using the production of cassava alcohol waste water - Google Patents
A kind of microbial manure and its application using the production of cassava alcohol waste water Download PDFInfo
- Publication number
- CN107840706A CN107840706A CN201710964813.6A CN201710964813A CN107840706A CN 107840706 A CN107840706 A CN 107840706A CN 201710964813 A CN201710964813 A CN 201710964813A CN 107840706 A CN107840706 A CN 107840706A
- Authority
- CN
- China
- Prior art keywords
- waste water
- cassava alcohol
- liquid
- fertilizer
- alcohol waste
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
- C05F5/008—Waste from biochemical processing of material, e.g. fermentation, breweries
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pest Control & Pesticides (AREA)
- Microbiology (AREA)
- Soil Sciences (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Environmental & Geological Engineering (AREA)
- Inorganic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The present invention relates to the ecological agriculture and field of environment protection, specifically discloses a kind of microbial manure of recycling cassava alcohol waste water production and its application in terms of agricultural non-point source pollution is prevented and treated.The present invention is used as fermentation medium by the use of cassava alcohol waste water, extensive fast culture bacillus subtilis, prepare the microbial manure for promoting plant growth, reducing loss of chemical fertilizer in soil, the technique productions cost is cheap, it is simple to operate, high concentration cassava alcohol waste water can also be made to get utilization, reduce disposal of pollutants.Microbial manure produced by the invention not only has antagonism plant disease, promotes plant growth and improves the function of crop quality, the function also with preventing and treating agricultural non-point source pollution.
Description
Technical field
The present invention relates to the ecological agriculture and environmental protection technical field, specifically, relates to the use of the life of cassava alcohol waste water
The microbial manure of production and its application in terms of agricultural non-point source pollution is prevented and treated.
Background technology
The content of starch of cassava is high, plants, be drought-resistant barren, arable land is not striven with cereal crops, cheap, is production second
The preferred raw materials of alcohol.Cassava alcohol can be widely applied to the industries such as medicine, chemical industry, food, and can be used as regenerative resource and clear
The clean energy, market potential are huge.But during using cassava fermenting and producing alcohol, it is organic that a large amount of high concentrations can be produced
Waste water (mg/litre of COD concentration numbers ten thousand), 1 ton of alcohol of production need to discharge about 15 tons of high-concentration waste water, and processing is up to standard very tired
Difficulty, processing cost is high, and manufacturing enterprise is difficult to bear.Pollution problem of the alcohol waste water to environment becomes increasingly conspicuous, it has also become restricts state
The bottleneck of interior Alcohol Production enterprise development.
Cassava alcohol waste water is mainly caused largely useless mash in alcohol production process, its mainly contain remaining starch,
Sugar, crude protein, cellulose, various inorganic salts and thalline material etc..Anaerobic-aerobic biological treatment method is currently used processing
Method, such as：Chinese invention patent (application number：201010167513.3) in disclose a kind of side of alcohol waste water anaerobic digestion
Method, reuse, solid-liquor separation, dry grain burning are concentrated by two-stage anaerobic, aerobic, anaerobic activated sludge separation, anaerobic activated sludge
With Sewage advanced treatment process.Chinese invention patent (application number：201110429555.4) in disclose a kind of cassava alcohol and give up
Water two-stage anaerobic processing system, collecting methane caused by two-stage anaerobic utilize, and then waste water enters back into follow-up aerobic section and continues to locate
Reason.Chinese invention patent (application number：201210000389.0) in disclose a kind of cassava alcohol waste water multi-level handling system, be
Anoxic 1-aerobic 1-anoxic 2-aerobic 2-membrane bioreactor group technology.Chinese invention patent (application number：
201210265698.0) a kind of cassava alcohol mixed waste liquor processing and reuse method are disclosed in, is cassava alcohol distillation process
Slops mixed and handled with the reclaimed water by Anaerobic Treatment and Aerobic Process for Treatment after be back to Cassava crushing process or
It is back to process of sizing mixing.Chinese invention patent (application number：201510420641.7) in disclose a kind of cassava alcohol waste water
Biological treatment, including：High temperature anaerobic processing, anaeration in normal temperature processing, membrane bioreactor, Aerobic Process for Treatment, nanofiltration depth
The processes such as reason.It can be seen that current existing handling process is all present, long flow path, processing cost are high, high-concentration waste water recycling is sharp
With rate it is low the shortcomings that, the simple and easy to do and good in economic efficiency utilization of wastewater resource technology of exploitation is still one very urgent
Task.
Bacillus subtilis (Bacillus subtilis) is a kind of aerobic, the bacillus of the degeneration-resistant spore of interior life, greatly
Small (0.8~1.2) μ m (1.5~4.0) μm, Gram-positive, pod membrane can be produced, raw in being given birth in gemma or being near, bacterium colony is thick
Rough, can liquefy gelatin, peptonized milk, reduce nitrate, hydrolysis starch, soil, lake, sea are widely present in for typical aerobic bacteria
Ocean and the body surface of animals and plants, itself is not pathogenic.Bacillus subtilis can be by successfully colonizing in plant rhizosphere, body surface or body
It is interior, suppress growth of pathogenic bacteria with the nutrition around pathogen competitive plant, secretion antibacterial material, while induce plant defense system
Pathogen invasion is resisted, so as to the effect with biological and ecological methods to prevent plant disease, pests, and erosion.Bacillus subtilis can also produce similar cell mitogen, plant life
The material of long hormone, promote the growth of plant.Such as：AgraQuest companies of the U.S. are opened with bacillus subtilis QST713 bacterial strains
Active bacteria formulation bactericide SerenadeTM is sent, and in the registration by EPA (EPA) in 2000, it is a variety of for preventing and treating
The diseases such as the powdery mildew of crop, frost and dew disease, epidemic disease, gray mold.MicroBios of the bacillus subtilis MBI600 in Britain
Group Ltd., the IdemistuKosanCo., Ltd. etc. of Japan have been registered, and have been carried out industrialization production, are used
In preventing and treating leaf diseases (gray mold, powdery mildew) and root disease (droop, root rot, black spot etc.).Bacillus subtilis
FZB24 is registered in countries such as Germany, the U.S., and is put into production in German Bayer companies, for preventing and treating Phytophthora infestans
Disease, gray mold and wheat powdery mildew, it is alternatively arranged as increasing production accelerator.As what bacillus subtilis was studied deepens continuously, hair
Existing its has extensively using value in industry, agricultural, medical and health, health care of food, aquaculture etc..
Chinese invention patent (application number：201210328716.5) in disclose a bacillus subtilis T-500 bacterial strains, protect
It is CGMCC No.6058 to hide numbering, to rice rice blast fungus, Rhizoctonia solani, rice leaf spot bacteria, Ustilaginoidea virens, water
The pathogens such as rice Xanthomonas campestris PV.oryzicola, Fusarium graminearum, aspergillus flavus and black-koji mould, stronger bacteriostatic activity is shown, and
There are preferable prevention and control to act on to its caused disease, fermentation medium composition includes：Carbon source (sucrose or starch), nitrogen source (soya bean
One or more of kind mixing in powder, beancake powder, corn oar), (beef leaches one in cream, yeast extract or fish meal trace element
Kind or two kinds of mixing).Chinese invention patent (application number：201210229066.9) in disclose one plant have bacteriostatic activity it is withered
Careless bacillus CX31 bacterial strains, deposit number are CCTCC No.M2012138, and the culture of the bacterial strain is trained using beef extract-peptone
Support base.Chinese invention patent (application number：201110205587.6) in disclose one plant of endophytic Bacillus subtilis, deposit number
For CGMCC No.4844, there is good prevention effect to pepper seasonal febrile diseases, cultivate culture medium that the bacterial strain uses for：PDA is cultivated
Base.
In summary, the bacterial strain report with promotion plant growth, the bacillus subtilis of antagonism plant disease is more, its
Cultural method is also different, and still, cost of material is generally higher.And cassava alcohol waste water is used as culture bacillus subtilis
The primary raw material of bacterium, saves cost of material, and report is also had no with the microbial manure prevention and control agricultural non-point source pollution of its production.
The content of the invention
In order to solve problems of the prior art, given birth to it is an object of the invention to provide one kind using cassava alcohol waste water
The microbial manure of production and its application in terms of agricultural non-point source pollution is prevented and treated.
In order to realize the object of the invention, technical scheme is as follows：
Bacterial strain used in the present invention is to promote plant life by the inventor's plant height that separation screening comes out from soil effect
Length, antagonistic phytopathogen, bacillus subtilis (Bacillus subtilis) the SZX11 bacterial strains for improving crop quality, the bacterium
Strain was deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 16th in September in 2013, and deposit number is：
CGMCC No.8188, depositary institution address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute, postcode：100101.The preservation information has been described in Publication No. CN105779323A, and publication date is 07 month 2016
In the Chinese patent application of 20 days.
The production method of multifunctional microbial fertilizer of the present invention is as follows：
1st, cassava alcohol Wastewater Pretreatment：It is 3-5 ten thousand that high concentration cassava alcohol waste water is diluted into COD concentration with running water
Mg/litre, then the pH to 7.0 with the cassava alcohol waste water after the regulation dilution of the plant ash rich in potassium carbonate of biomass thermal power plant
~8.5, sterilized 10~30 minutes in 110~121 DEG C, standing sedimentation, to less than 40 DEG C；Supernatant is bacillus SZX11
The fluid nutrient medium of bacterial strain；Sediment is used for the solid medium for preparing bacillus SZX11 bacterial strains.
2nd, prepared by liquid seeds：It is inoculated with into the triangular flask equipped with the cassava alcohol wastewater liquid culture medium pre-processed oblique
The bacillus subtilis SZX11 bacterial strains of face preservation, in 30~40 DEG C, 120~200 revs/min of shaken cultivations 24~36 hours, obtain
Liquid seeds.
3rd, liquid state fermentation：It is to equipped with inoculation volume ratio in the cassava alcohol wastewater liquid culture medium fermentation tank pre-processed
2%~10% liquid seeds, in 25~40 DEG C, lead to filtrated air culture 15~36 hours；Cultured zymotic fluid is through flat board
Count of bacteria, viable count reach 2 × 109It is more than CFU/ milliliters.
4th, the preparation of liquid microbe fertilizer：Into the 3rd cultured zymotic fluid of step by weight addition 0.5%~2%
Glycerine and 0.1%~0.5% Tween 80, be well mixed, liquid microbe fertilizer can be obtained, it is filling, be stored in shady and cool drying
Place.
5th, the preparation of solid microbe fertilizer：The sediment for the cassava alcohol waste water that 1st step is obtained and stalk or livestock and poultry
Excrement is according to carbon-nitrogen ratio 15~25:1 is well mixed, and solid medium is made, and moisture 60%~70% is adjusted, according to weight
Than the inoculum concentration for 2%, 3 cultured zymotic fluid of inoculation step, aerobic fermentation 10-15 days is fully decomposed, obtains organic fertilizer；
Using fully decomposed organic fertilizer as carrier, according to zymotic fluid: the weight ratio of organic fertilizer 1: 3~1: 10, zymotic fluid is added to
In organic fertilizer, it is sufficiently mixed uniformly, aeration-drying to water content obtains solid microbe fertilizer below 30%, pack, storage
At shady and cool drying.
The application method that microorganism Bacillus subtilis fertilizer of the present invention is used to prevent and treat agricultural non-point source pollution is as follows：
1st, liquid microbe fertilizer is diluted with water to viable count as 5 × 106CFU/ milliliter~5 × 107CFU/ milliliters, mix
Close uniformly, applied according to the dosage ditch of every mu of 1~5 cubic metre of dilution in the soil near crop root；Reduce routine simultaneously
Using the amount 0~50% of chemical fertilizer, nitrogen loss in farmland 20%~50% can be reduced.
2nd, during solid microbe fertilizer is manured into soil according to 0.3~3 ton every mu of dosage as base manure, or in crop life
In long-term, top dressing is applied；Routine is reduced simultaneously and applies chemical fertilizer 0~60%, can reduce nitrogen loss in farmland 30%~70%.
The beneficial effects of the present invention are：
The invention provides a kind of microbial manure of recycling cassava alcohol waste water production and its in preventing and treating agricultural
Application in terms of pollution of area source.The present invention is used as fermentation medium, extensive fast culture withered grass bud by the use of cassava alcohol waste water
Spore bacillus, the microbial manure for promoting plant growth, reducing loss of chemical fertilizer in soil is prepared, the technique productions cost is cheap, behaviour
Make simply, high concentration cassava alcohol waste water can also be made to get utilization, reduce disposal of pollutants.Micro- life produced by the invention
Thing fertilizer is used for the production of vegetables, can not only improve the yield and quality, and can improve soil environment quality, prevents from source
Agricultural non-point source pollution is controlled, shows wide application prospect.The microbial manure not only has antagonism plant disease, promotes plant
Growth and the function of improving crop quality, also there is the function of preventing and treating agricultural non-point source pollution.
Embodiment
The preferred embodiment of the present invention is described in detail below in conjunction with embodiment.It is it will be appreciated that following real
Providing merely to play the purpose of explanation for example is applied, is not used to limit the scope of the present invention.The skill of this area
Art personnel can carry out various modifications and replacement in the case of without departing substantially from spirit of the invention and spirit to the present invention.
Experimental method used in following embodiments is conventional method unless otherwise specified.
Material used, reagent etc., unless otherwise specified, are commercially obtained in following embodiments.
The bacterial strain that the present invention uses is that the plant height come out from plant surface separation screening imitates plant growth-promoting bacteria --- withered grass
Bacillus (Bacillus subtilis) SZX11 bacterial strains, the bacterial strain are deposited in China Committee for Culture Collection of Microorganisms
Common micro-organisms center, deposit number are：CGMCC No.8188.
Bacillus subtilis biocontrol agent provided by the present invention can effectively prevent and treat a variety of soilborne fungal pathogens, and can promote
Enter absorption and utilization of the crop to nutrient, moreover it is possible to provide a certain amount of potash fertilizer.Technical scheme provided by the invention produces agricultural
Microbial bacterial agent is applied to the usage amount that agricultural production can reduce chemical fertilizer and agricultural chemicals, so as to improve farm environment quality, preventing and treating
Agricultural non-point source pollution；And production cost is very low, high-concentration waste water is got utilization, reduce pollutant emission.Specifically
Embodiment is as follows：
The cassava alcohol waste water of embodiment 1 produces bacillus subtilis liquid microbe fertilizer
Cassava alcohol Wastewater Pretreatment：Arrived with the pH of the regulation waste water of the plant ash rich in potassium carbonate of biomass thermal power plant
7.8 or so, sterilized 15 minutes in 115 DEG C.
It is prepared by liquid seeds：It is prepared by liquid seeds：It is inoculated with into the triangular flask equipped with the cassava alcohol waste water pre-processed
The bacillus subtilis of slant preservation, in 35 DEG C, 150 revs/min of shaken cultivations 24 hours, obtain liquid seeds.
Liquid state fermentation：To equipped with the liquid strain that inoculation volume ratio is 5% in the cassava alcohol wastewater fermentation tank pre-processed
Son, in 35 DEG C, lead to filtrated air culture 20 hours；Cultured zymotic fluid through count plate, viable count reaches 2 ×
109It is more than CFU/ milliliters.
The preparation of liquid microbe fertilizer：Into cultured zymotic fluid by weight the glycerine and 0.2% for adding 1.5%
Tween 80, be well mixed, liquid microbe fertilizer can be obtained, it is filling, be stored at shady and cool dry.
The cassava alcohol waste water of embodiment 2 produces bacillus subtilis solid microbe fertilizer
Cassava alcohol Wastewater Pretreatment：Arrived with the pH of the regulation waste water of the plant ash rich in potassium carbonate of biomass thermal power plant
7.8 or so, sterilized 20 minutes in 115 DEG C.
It is prepared by liquid seeds：It is prepared by liquid seeds：It is inoculated with into the triangular flask equipped with the cassava alcohol waste water pre-processed
The bacillus subtilis of slant preservation, in 37 DEG C, 150 revs/min of shaken cultivations 20 hours, obtain liquid seeds.
Liquid state fermentation：To equipped with the liquid strain that inoculation volume ratio is 3% in the cassava alcohol wastewater fermentation tank pre-processed
Son, in 37 DEG C, lead to filtrated air culture 18 hours；Cultured zymotic fluid through count plate, viable count reaches 2 ×
109It is more than CFU/ milliliters.
The preparation of solid microbe fertilizer：Using fully decomposed organic fertilizer as carrier, according to zymotic fluid: organic fertilizer 1: 5
Weight ratio, zymotic fluid is added in organic fertilizer, be sufficiently mixed uniformly, aeration-drying to water content obtains below 30%
Solid microbe fertilizer, packaging, it is stored at shady and cool dry.
The microorganism Bacillus subtilis fertilizer of embodiment 3 is used to prevent and treat agricultural non-point source pollution
Tested using green house of vegetables cell, 8 identical hillslope processes of condition, each cell 60m are set2, it is 2 small
Area is 1 group of parallel test, is divided into 4 groups, plants intensive shadow on lung, in intensive shadow on lung Seedling Stage, the 1st group of 2 cells are not applied fertilizer and compared；
2nd group of 2 cells respectively apply 1 × 107CFU/ grams of 100 kilograms of bacillus subtilis solid microbe fertilizer is used as base manure,
Rape complete germination respectively imposes one time 1 × 10 after 3 weeks7The dilution of the bacillus subtilis liquid microbe fertilizer of CFU/ milliliters
Liquid；3rd group of 2 cells respectively apply urea once according to 15 kgs/acre of dose after rape complete germination and after 3 weeks；4th
Group 2 respectively applies urea once according to 15 kgs/acre of dose after rape complete germination and after 3 weeks, and according to the 2nd prescription
Formula applies microbial manure, and each cell identical is irrigated and way to manage.Growth of rape investigates yield and quality after 5 weeks, simultaneously
The nitrogen content remained in measure soil.
As a result show, microbial manure (the 2nd group of experiment) is administered alone and the rape of urea (the 3rd group of experiment) is administered alone
Yield is basically identical, all check plot (the 1st group of experiment) volume increase 50% or so than not applying fertilizer；Two kinds of fertilizer all apply (the 4th group
Experiment) ratio check plot volume increase 90% or so；NO3-N and NO2-N content is micro- not apply fertilizer and be administered alone in rape
Bacteria agent it is minimum, be below the 1/3 of national standard Limited Doses, using after urea again using microbial bacterial agent also below state
The 1/2 of family's standard limitation, and it is all obvious exceeded that NO3-N and NO2-N content in the rape of urea is administered alone.4th group real
Test in the soil of cell, the content of nitrate nitrogen, ammonium nitrogen and nitrite nitrogen is substantially less than the soil of the 3rd group of hillslope processes, table
It is bright to apply microbial manure, the yield and quality of rape can not only be improved, and be advantageous to the stream of prevention and control nitrogen in farmland soil
Lose, agricultural non-point source pollution can be prevented and treated from source.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (5)
1. a kind of microbial manure produced using cassava alcohol waste water, it is characterised in that its preparation method comprises the following steps：
S1, cassava alcohol Wastewater Pretreatment：It is the millis of 3-5 ten thousand that high concentration cassava alcohol waste water is diluted into COD concentration with running water
G/l, then with the pH to 7.0 of the cassava alcohol waste water after plant ash regulation dilution of the biomass thermal power plant rich in potassium carbonate~
8.5, sterilized 10~30 minutes in 110~121 DEG C, standing sedimentation, to less than 40 DEG C；Supernatant is the liquid of Bacillus strain
Body culture medium；Sediment is used for the solid medium for preparing Bacillus strain；
It is prepared by S2, liquid seeds：Inclined-plane is inoculated with into the triangular flask equipped with the cassava alcohol wastewater liquid culture medium pre-processed
The bacillus subtilis strain of preservation, in 30~40 DEG C, 120~200 revs/min of shaken cultivations 24~36 hours, obtain liquid strain
Son；
S3, liquid state fermentation：To equipped with the cassava alcohol wastewater liquid culture medium fermentation tank pre-processed be inoculated with volume ratio be 2%
~10% liquid seeds, in 25~40 DEG C, lead to filtrated air culture 15~36 hours；Cultured zymotic fluid is through flat board bacterium
Count, viable count reaches 2 × 109It is more than CFU/ milliliters；
S4, liquid microbe fertilizer preparation：Into the cultured zymotic fluids of S3 by weight the glycerine for adding 0.5%~2%
Tween 80 with 0.1%~0.5%, is well mixed, can obtain liquid microbe fertilizer, filling, is stored at shady and cool dry；
Or the preparation of solid microbe fertilizer：By the sediment of the obtained cassava alcohol waste water of S1 and stalk or feces of livestock and poultry according to
Carbon-nitrogen ratio 15~25:1 is well mixed, and solid medium is made, and adjusts moisture 60%~70%, is 2% according to weight ratio
Inoculum concentration, 3 cultured zymotic fluid of inoculation step, aerobic fermentation 10-15 days, fully it is decomposed, obtain organic fertilizer；With fully rotten
Ripe organic fertilizer is carrier, according to zymotic fluid: the weight ratio of organic fertilizer 1: 3~1: 10, and the cultured zymotic fluids of S3 are added
Into organic fertilizer, it is sufficiently mixed uniformly, aeration-drying to water content obtains solid microbe fertilizer below 30%, packaging, deposit
It is placed at shady and cool dry.
2. microbial manure according to claim 1, it is characterised in that the Bacillus strain is bacillus
SZX11 bacterial strains, deposit number are CGMCC No.8188.
3. application of the microbial manure in agricultural non-point source pollution is prevented and treated described in claim 1 or 2.
4. application according to claim 3, it is characterised in that liquid microbe fertilizer is diluted with water to viable count as 5
×106CFU/ milliliter~5 × 107CFU/ milliliters, it is well mixed, applies and make according to the dosage ditch of every mu of 1~5 cubic metre of dilution
In soil near thing root.
5. application according to claim 3, it is characterised in that by solid microbe fertilizer according to 0.3~3 ton every mu of use
During amount is manured into soil as base manure, or in crop growth period, top dressing is applied.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710964813.6A CN107840706A (en) | 2017-10-17 | 2017-10-17 | A kind of microbial manure and its application using the production of cassava alcohol waste water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710964813.6A CN107840706A (en) | 2017-10-17 | 2017-10-17 | A kind of microbial manure and its application using the production of cassava alcohol waste water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107840706A true CN107840706A (en) | 2018-03-27 |
Family
ID=61662486
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710964813.6A Pending CN107840706A (en) | 2017-10-17 | 2017-10-17 | A kind of microbial manure and its application using the production of cassava alcohol waste water |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107840706A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110437017A (en) * | 2019-09-10 | 2019-11-12 | 中国科学院生态环境研究中心 | A kind of method that feces of livestock and poultry produces microbial manure and controls agricultural non -point pollution |
| CN111170800A (en) * | 2019-12-30 | 2020-05-19 | 江苏联海生物科技有限公司 | Fertilizing method for processing biological organic fertilizer in waste by using fuel ethanol |
| CN111960870A (en) * | 2020-08-06 | 2020-11-20 | 南阳市京德啤酒技术开发有限公司 | Method for producing microbial fertilizer by using whisky distillation residual liquid |
| CN114436705A (en) * | 2022-03-18 | 2022-05-06 | 河南省驻科生物科技有限公司 | Liquid fertilizer for increasing yield and preventing and treating plant diseases and insect pests and preparation method thereof |
| CN114940635A (en) * | 2022-06-08 | 2022-08-26 | 中资蓝天生态环境科技有限公司 | Method for reducing emission of greenhouse gas by organic solid waste semipermeable membrane fermentation and preparing microbial fertilizer |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102942393A (en) * | 2012-12-16 | 2013-02-27 | 王天喜 | Method for fermenting and producing microbial agent by utilizing cassava alcohol residues |
| WO2016055879A1 (en) * | 2014-08-28 | 2016-04-14 | Universidad Eafit | Process for increasing biomass and spores production of plant growth promoting bacteria of the bacillus genus |
| CN105624056A (en) * | 2014-11-06 | 2016-06-01 | 中国科学院生态环境研究中心 | Plant growth promoting bacteria separated from phyllosphere, and production method of bacterial agent of plant growth promoting bacteria |
| CN105779323A (en) * | 2014-12-23 | 2016-07-20 | 中国科学院生态环境研究中心 | Bacillus subtilis capable of efficiently degrading quorum sensing signal molecules and production method of inoculant thereof |
| CN107129327A (en) * | 2017-04-11 | 2017-09-05 | 岳阳绿光农业科技有限公司 | Microbial manure and preparation method thereof |
-
2017
- 2017-10-17 CN CN201710964813.6A patent/CN107840706A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102942393A (en) * | 2012-12-16 | 2013-02-27 | 王天喜 | Method for fermenting and producing microbial agent by utilizing cassava alcohol residues |
| WO2016055879A1 (en) * | 2014-08-28 | 2016-04-14 | Universidad Eafit | Process for increasing biomass and spores production of plant growth promoting bacteria of the bacillus genus |
| CN105624056A (en) * | 2014-11-06 | 2016-06-01 | 中国科学院生态环境研究中心 | Plant growth promoting bacteria separated from phyllosphere, and production method of bacterial agent of plant growth promoting bacteria |
| CN105779323A (en) * | 2014-12-23 | 2016-07-20 | 中国科学院生态环境研究中心 | Bacillus subtilis capable of efficiently degrading quorum sensing signal molecules and production method of inoculant thereof |
| CN107129327A (en) * | 2017-04-11 | 2017-09-05 | 岳阳绿光农业科技有限公司 | Microbial manure and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 梅雪臣 等: "利用木薯酒糟液培养枯草芽孢杆菌的工艺优化", 《中国酿造》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110437017A (en) * | 2019-09-10 | 2019-11-12 | 中国科学院生态环境研究中心 | A kind of method that feces of livestock and poultry produces microbial manure and controls agricultural non -point pollution |
| CN111170800A (en) * | 2019-12-30 | 2020-05-19 | 江苏联海生物科技有限公司 | Fertilizing method for processing biological organic fertilizer in waste by using fuel ethanol |
| CN111960870A (en) * | 2020-08-06 | 2020-11-20 | 南阳市京德啤酒技术开发有限公司 | Method for producing microbial fertilizer by using whisky distillation residual liquid |
| CN114436705A (en) * | 2022-03-18 | 2022-05-06 | 河南省驻科生物科技有限公司 | Liquid fertilizer for increasing yield and preventing and treating plant diseases and insect pests and preparation method thereof |
| CN114940635A (en) * | 2022-06-08 | 2022-08-26 | 中资蓝天生态环境科技有限公司 | Method for reducing emission of greenhouse gas by organic solid waste semipermeable membrane fermentation and preparing microbial fertilizer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101659932B (en) | Antagonistic bacteria preventing and removing continuous cropping tobacco bacterial wilt and microbial organic fertilizer thereof | |
| CN101659934B (en) | Antagonistic bacteria preventing and removing continuous cropping banana Panama wilt disease and microbial organic fertilizer thereof | |
| CN100509709C (en) | Process of twice fermenting garbage-sludge mixture to produce microbial fertilizer | |
| CN103820349B (en) | One bacillus licheniformis and the production method of microbial inoculum thereof and purposes | |
| CN105695354B (en) | The technique and application of superhigh temperature aerobic composting fermentation processing municipal sludge | |
| CN102093975B (en) | Composite fungicide for rapid degradation of organic waste and applications thereof | |
| CN103194405B (en) | Growth-promoting bacteria for promoting ginger growth and preventing and controlling continuous cropping ginger soil-borne wilt and microorganism organic fertilizer produced from growth-promoting bacteria | |
| CN112375720B (en) | Bacillus subtilis and application thereof | |
| CN103937695B (en) | A kind of composite bacteria agent and manufacture method thereof of processing livestock and poultry cultivation sewage | |
| CN101973795A (en) | Sludge aerobic composting method by utilizing composting compound microbial inoculum | |
| CN107840706A (en) | A kind of microbial manure and its application using the production of cassava alcohol waste water | |
| CN103695317B (en) | There is the production method of the efficient phosphate-solubilizing penicillium oxalicum microbial inoculum of heavy metal tolerance characteristic | |
| CN104609995A (en) | Plant growth promoting bio-organic fertilizer for saline-alkali land | |
| CN103484396B (en) | New strain of streptomyces thermocarboxydus and application thereof | |
| CN105948842B (en) | Livestock and poultry dung recovery produces lichens micro-organisms bacillus fertilizer | |
| CN101244955A (en) | Biological fertilizer and manufacture method thereof | |
| CN101724596A (en) | Method for culturing organophosphorus pesticide degrading bacteria | |
| CN103642703B (en) | There is the production method of the efficient phosphate-solubilizing aspergillus japonicus microbial inoculum of heavy metal tolerance characteristic | |
| CN101886055B (en) | Antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt | |
| CN102409014A (en) | Winter jujube rhizosphere growth promoting bacillus subtilis and application thereof | |
| CN107129327A (en) | Microbial manure and preparation method thereof | |
| CN101215532B (en) | Bacillus megaterium and its application and application method in ferment bacteria | |
| CN104016733B (en) | Yeast wastewater resource produces multifunctional biological fertilizer | |
| CN103931660A (en) | Method utilizing critic acid wastewater to produce bacillus amyloliquefaciens bio-control inoculant | |
| CN103173387B (en) | Growth-promoting bacteria for facilitating growth of rape and microbial organic fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20220124 Address after: 18 Shuangqing Road, Haidian District, Beijing 100089 Applicant after: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences Applicant after: Chinese funded Lantian Ecological Environment Technology Co., Ltd Address before: 100085 Beijing city Haidian District Shuangqing Road No. 18 Applicant before: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences |
|
| TA01 | Transfer of patent application right |