CN105315997A - Composite microbial soil repairing agent, and preparation and usage methods thereof - Google Patents
Composite microbial soil repairing agent, and preparation and usage methods thereof Download PDFInfo
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Abstract
The invention relates to a composite microbial soil repairing agent. The invention also relates to a preparation method for the composite microbial soil repairing agent. The composite microbial soil repairing agent is formed by mixing the following compositions in percent by volume: 5-10 of photosynthetic bacterium fermentation liquid, 20-25 of bacillus subtilis fermentation liquid, 20-25 of plant lactobacillus fermentation liquid, 20-25 of microzyme fermentation liquid, and 20-25 of enterococcus faecalis fermentation liquid. The five bacterial strains can be subjected to secondary enlarged culture under proper conditions of temperature, throughput, medium, acid-base value and culture time, and the culture processes are controlled in fermentation tanks free of infectious microbes. The composite microbial soil repairing agent brings beneficial microbes into soil, transfers, absorbs, degrades and converts pollutants in soil by utilizing a microbial technology, adjusts soil pH value, eliminates soil hardening, inhibits soil-borne diseases, reaches the purpose of improving soil composition, and enables soil to recover the original ecological structure.
Description
Technical field
The invention belongs to technical field of biological fertilizer, be specifically related to a kind of complex microorganism soil-repairing agent and its preparation method and application.
Background technology
Soil is the basic resource that the mankind depend on for existence, is the important place of terrestrial ecosystem material, energy exchange and circulation.Soil once be polluted, on the impact of environment be continue and irreversible.Soil environment quality and ecological safety are the important guarantees of sustainable development, along with industrial and agricultural development, China's edatope goes from bad to worse, and soil resource bears the increasing pressure, current China, by nearly 2,000 ten thousand hectares of the cultivated area of heavy metal contamination, accounts for 1/5 of total cultivated area.Reach 2,000,000 hectares by mining area contaminated land, about 5,000,000 hectares, petroleum pollution soil, solid waste is stacked and is polluted about 50,000 hectares, and " three industrial wastes " pollute and plough nearly 1,000 ten thousand hectares, and the farmland area of sewage irrigation reaches more than 330 ten thousand hectares.Soil pollution makes National Agricultural grain drop in production more than 1,300 ten thousand tons, and because of agricultural chemicals and Organic pollutants, radiocontamination, the financial loss that the pollution of the other types such as pathogen contamination causes is difficult to estimate.Owing to polluting, the trophic function of soil, purification function, pooling feature and organic support function are lost, in the urgent need to repairing and administering.Carry out the research and apply of contaminated soil generating process and regulation and control, contaminated soil remediation in a deep going way, tightly grasp the direction of contaminated soil remediation technological innovation, be directly connected to the ecological safety of China.
Summary of the invention
One of the object of the invention is to provide a kind of complex microorganism soil-repairing agent;
Two of the object of the invention is to provide a kind of method preparing above-mentioned complex microorganism soil-repairing agent;
Three of the object of the invention is reparations mentioned microorganism soil-repairing agent being applied to soil;
Technical scheme of the present invention is as follows: a kind of effectively compound microbial soil-repairing agent, and the zymocyte liquid of photosynthetic bacterium, subtilis, plant lactobacillus, yeast and enterococcus faecalis mixes; The volume percent of each zymocyte liquid is: photosynthetic bacterium 5-10%, subtilis 20-25%, plant lactobacillus 20-25%, yeast 20-25%, enterococcus faecalis 20-25%.
Described photosynthetic bacterium is preferably the red false first spore bacterium in marsh; Described yeast is preferably yeast saccharomyces cerevisiae.
The preparation method of above-mentioned effectively compound microbial soil-repairing agent, adopts and symbol strain bacterium is carried out composite method system after pure culture in without the fermentor tank of miscellaneous bacteria respectively joins, the method cultivation that the pure culture process of every strain bacterium is all expanded by secondary; The method comprises the steps:
(1) respectively the mother of photosynthetic bacterium, subtilis, yeast, plant lactobacillus and enterococcus faecalis is planted and be inoculated in one-level and cultivate and very carry out one-level cultivation, obtain first class inoculum;
(2) respectively the first class inoculum of photosynthetic bacterium, subtilis, yeast, plant lactobacillus and enterococcus faecalis is inoculated in secondary to cultivate and more carry out secondary cultivation, obtains zymocyte liquid;
(3) symbol zymocyte liquid is mixed in proportion.
When step (1) photosynthetic bacterium one-level is cultivated, substratum is KH
2pO
41.0g, CaCl0.1g, NaHCO
33.0g, sodium acetate 1.0g, MgCl
20.5g, NH
4cl1.0g, NaCl1.0g, trace element solution 1.0ml, vitamin solution 1.0ml, sodium succinate 1.0g, yeast extract 0.5g, peptone 0.5g, distilled water 1.0L, inoculum size is 1.5-5%, cultivating pH value is 6.8, culture temperature is 30 DEG C, intensity of illumination is 3500-4500 lux, and incubation time is 5-8 days;
When subtilis one-level is cultivated, substratum is peptone 10.0g, beef leaching thing 3.0g, NaCl5.0g, distilled water 1.0L, and inoculum size is 1.5-5%, and cultivating pH value is 7.0, and culture temperature is 37 DEG C, and incubation time is 1-2 days;
When yeast one-level is cultivated, substratum is glucose 10.0g, peptone 10.0g, yeast extract 5g, agar 20g, distilled water 1.0L, and inoculum size is 1.5-5%, and cultivation pH value is 5-7, and culture temperature is 25-28 DEG C, and incubation time is 1-2 days;
When plant lactobacillus one-level is cultivated, substratum is glucose 5.0g, casein peptone 10.0g, extractum carnis 10.0g, yeast powder 5.0g, sodium acetate 5.0g, dibasic ammonium citrate 2.0g, tween 80 1.0g, K
2hPO
42.0g, MgSO
47H
2o0.2g, MnSO
4h
2o0.05g, CaCO
320.0g, agar 15.0g, distilled water 1.0L, inoculum size is 1.5-5%, and cultivating pH value is 6.8, and culture temperature is 30 DEG C, and incubation time is 3-5 days;
When enterococcus faecalis one-level is cultivated, substratum is glucose 5.0g, casein peptone 10.0g, extractum carnis 10.0g, yeast powder 5.0g, sodium acetate 5.0g, dibasic ammonium citrate 2.0g, tween 80 1.0g, K
2hPO
42.0g, MgSO
47H
2o0.2g, MnSO
4h
2o0.05g, CaCO
320.0g, agar 15.0g, distilled water 1.0L, inoculum size is 1.5-5%, and cultivating pH value is 6.8, and culture temperature is 30 DEG C, and incubation time is 3-5 days.
In step (2), photosynthetic bacterium secondary is cultivated as intermittent type aerated culture, and substratum is identical with one-level, and inoculum size is 10%-15%, and cultivating pH value is 6.8, and culture temperature is 30 DEG C, and intensity of illumination is 3000-4000 lux, and incubation time is 6-8 days;
Subtilis secondary is cultivated and is preferably aerated culture, and substratum is identical with one-level, and inoculum size is 10%-15%, and cultivating pH value is 7.0, and culture temperature is 37 DEG C, and incubation time is 1-2 days;
Yeast secondary is cultivated and is preferably aerated culture, and substratum is identical with one-level, and inoculum size is 10%-15%, and cultivation pH value is 5-7, and culture temperature is 25-28 DEG C, and incubation time is 1-2 days;
Plant lactobacillus secondary is cultivated and is preferably intermittent type aerated culture, and when secondary is cultivated, substratum is identical with one-level, and inoculum size is 10%-15%, and cultivating pH value is 6.8, and culture temperature is 30 DEG C, and incubation time is 5-7 days;
Enterococcus faecalis secondary is cultivated and is preferably intermittent type aerated culture, and when secondary is cultivated, substratum is identical with one-level, and inoculum size is 10%-15%, and cultivating pH value is 6.8, and culture temperature is 30 DEG C, and incubation time is 5-7 days.
Present invention also offers the described application of complex microorganism soil-repairing agent in rehabilitating soil.
Present invention also offers the described application of complex microorganism soil-repairing agent in tomato, grape, rapeseed cultivation.
Present invention also offers the using method of described complex microorganism soil-repairing agent, it is characterized in that foliage-spray and fill with root, consumption is 1-2kg/ mu.
Present invention also offers described complex microorganism soil-repairing agent itself containing mass efficient viable bacteria, just can be absorbed by crops as nutrient in being manured into soil, residue can not be formed in the ground.Simultaneously by a series of microbial activities such as growth, breeding, death of bacterial classification itself, for soil creates soil ulmin, increase the organic content in soil, improve the fertility of soil.This series of microbial activities also can produce a large amount of " phosphoglycan ", help to decompose in the middle of soil due to long-term a large amount of use chemical fertilizer to cause solidification and calcified chemical residue, and make soil become loose, oxygen G&W etc. is circulated, make the soil restoration vigour and vitality in the past hardened, for the life of roots of plants Gao is createing loose environment, make crop root ramp, deeply prick.Inorganic nitrogen, phosphorus, potassium and the trace element etc. be deposited in all the year round in soil are made to be able to fully by plant root is absorbed, simultaneously, make unsound be in morbid state soil got well, recover self from wound power and reproducibility, make it return to the Soil structure of ecosystem.These act on the result brought is make agricultural output high, and quality is good, quality better, beautiful outline form, and keeping quality is strong, and the fertility of soil is protected and strengthened.This efficient function is that other fertilizer is unexistent, and in thin and weak ground or the ground that hardens, the serious soil of disease and pest, use this complex microorganism soil-repairing agent, effect is more obvious.
Advantageous Effects embodies as follows:
For farm crop provide growth necessary various nutritive element after using the present invention, and it is active to increase beneficial microorganism in soil, overcomes the excessive of chemical fertilizer simultaneously and uses and the uneven drawback caused of applying fertilizer.Excessive chemical fertilizer of using can damage natural, ecological, and as the increase of nitrate nitrogen content in environment, the secondary salinization of protecting field, the destruction of some Soil structure, on the impact of veterinary antibiotics quality, finally causes reduction and the Soil degradation of chemical fertilizer rate of returns.
This invention is responsible for again the important task that soil organism soil ulmin transforms, increase soil aggregate, improve preserve moisture and fertility ability, activate the nutrient be cured by the soil, improve chemical fertilizer utilization ratio, beneficial microorganism simultaneously in microbe soil restoration agent secretes various benefit materials to soil, raw at yield stimulant, indolylacetic acid, Plant hormones regulators,gibberellins and various enzyme, thus effectively promote the conversion of nutrient, alleviate the generation of soil-borne disease, repair contaminated soil, be the desirable fertilizer of green agriculture, Organic farming, in agricultural sustainable development, have bright prospects.
Embodiment
5 kinds of bacterial classifications: the red false first spore bacterium in marsh, subtilis, yeast saccharomyces cerevisiae, plant lactobacillus and enterococcus faecalis.
Below be respectively the secondary culturing process of five strain bacterium:
The red false first spore bacterium in marsh: when one-level is cultivated, substratum is KH
2pO
41.0g, CaCl0.1g, NaHCO
33.0g, sodium acetate 1.0g, MgCl
20.5g, NH
4cl1.0g, NaCl1.0g, trace element solution 1.0ml, vitamin solution 1.0ml, sodium succinate 1.0g, yeast extract 0.5g, peptone 0.5g, distilled water 1.0L, inoculum size is 1.5-5%, cultivating pH value is 6.8, culture temperature is 30 DEG C, intensity of illumination is 3500-4500 lux, and incubation time is 5-8 days; Secondary is cultivated as intermittent type aerated culture, and substratum is identical with one-level, and inoculum size is 10%-15%, and cultivating pH value is 6.8, and culture temperature is 30 DEG C, and intensity of illumination is 3000-4000 lux, and incubation time is 6-8 days.
When subtilis one-level is cultivated, substratum is peptone 10.0g, beef leaching thing 3.0g, NaCl5,0g, distilled water 1.0L, and inoculum size is 1.5-5%, and cultivating pH value is 7.0, and culture temperature is 37 DEG C, and incubation time is 1-2 days; Secondary is cultivated and is preferably aerated culture, and substratum is identical with one-level, and inoculum size is 10%-15%, and cultivating pH value is 7.0, and culture temperature is 37 DEG C, and incubation time is 1-2 days.
When yeast saccharomyces cerevisiae one-level is cultivated, substratum is glucose 10.0g, peptone 10.0g, yeast extract 5g, agar 20g, distilled water 1.0L, and inoculum size is 1.5-5%, and cultivation pH value is 5-7, and culture temperature is 25-28 DEG C, and incubation time is 1-2 days; Secondary is cultivated and is preferably aerated culture, and substratum is identical with one-level, and inoculum size is 10%-15%, and cultivation pH value is 5-7, and culture temperature is 25-28 DEG C, and incubation time is 1-2 days.
When plant lactobacillus one-level is cultivated, substratum is glucose 5.0g, casein peptone 10.0g, extractum carnis 10.0g, yeast powder 5.0g, sodium acetate 5.0g, dibasic ammonium citrate 2.0g, tween 80 1.0g, K
2hPO
42.0g, MgSO
47H
2o0.2g, MnSO
4h
2o0.05g, CaCO
320.0g, agar 15.0g, distilled water 1.0L, inoculum size is 1.5-5%, and cultivating pH value is 6.8, and culture temperature is 30 DEG C, and incubation time is 3-5 days; Secondary is cultivated and is preferably intermittent type aerated culture, and when secondary is cultivated, substratum is identical with one-level, and inoculum size is 10%-15%, and cultivating pH value is 6.8, and culture temperature is 30 DEG C, and incubation time is 5-7 days.
When enterococcus faecalis one-level is cultivated, substratum is glucose 5.0g, casein peptone 10.0g, extractum carnis 10.0g, yeast powder 5.0g, sodium acetate 5.0g, dibasic ammonium citrate 2.0g, tween 80 1.0g, K
2hPO
42.0g, MgSO
47H
2o0.2g, MnSO
4h
2o0.05g, CaCO
320.0g, agar 15.0g, distilled water 1.0L, inoculum size is 1.5-5%, and cultivating pH value is 6.8, and culture temperature is 30 DEG C, and incubation time is 3-5 days; Secondary is cultivated and is preferably intermittent type aerated culture, and when secondary is cultivated, substratum is identical with one-level, and inoculum size is 10%-15%, and cultivating pH value is 6.8, and culture temperature is 30 DEG C, and incubation time is 5-7 days.
Its implementing procedure is as follows
The first step: tiltedly transfer on the eggplant bottle inclined-plane of 500 liters by the test tube of five strain bacterium respectively, every strain bacterium is transferred 2 eggplant bottles, is filled on whole inclined-plane, cultivates stand-by in incubator.
Second step: respectively by the first step cultured eggplant bottle inclined-plane by described one-level cultivate inoculum size be inoculated into 100L one grade fermemtation tank (substratum in fermentor tank according in summary of the invention and substratum configuration, the one-level culture temperature of each strain bacterium is cooled to respectively after 121 degree of sterilizing half an hour), and cultivate described culture parameters according to one-level and cultivate the fixed time.
3rd step: (substratum of second order fermentation tank is prepared by described secondary medium respectively one-level nutrient solution cultured in second step to be inoculated into respectively the second order fermentation tank of 1000L, the secondary culture temperature of each strain bacterium is cooled to respectively after 121 degree of sterilizing half an hour), and cultivate described culture parameters according to secondary and cultivate the fixed time.
4th step: five strain bacterium mix by the ratio being respectively 10%, 20%, 25%, 20% and 25% with, plant lactobacillus and enterococcus faecalis according to photosynthetic bacterium, subtilis, yeast.
After five strain bacterium are cultivated respectively by above-mentioned secondary, subtilis can reach 6,000,000,000/ml, yeast can reach 1,000,000,000/ml, photosynthetic bacterium can reach 3,500,000,000/ml, plant lactobacillus reaches 800,000,000/ml, enterococcus faecalis can reach 700,000,000/ml, and composite rear bacterium number can reach more than 50 second, and the current Ministry of Agriculture is 200,000,000/ml to the standard of agriculture microbiobacterial agent.
Present invention also offers the using method of described complex microorganism soil-repairing agent in rehabilitating soil and be applied in the positive effect in tomato, grape, rapeseed cultivation.
Present invention also offers described complex microorganism soil-repairing agent for foliage-spray and filling root, consumption is 1-2kg/ mu.Impose in soil, increase the organic content in soil, improve the fertility of soil; Reach potassium decomposing, release phosphorus, the object of fixed nitrogen, the soil hardened is improved rapidly; Make unsound be in morbid state soil restoration self from wound power and reproducibility, make it return to the Soil structure of ecosystem.Be applied in the plant husbandry such as tomato, grape, rape, the consumption of chemical fertilizer 30-50% can be reduced, reduce the consumption of agricultural chemicals, make crop root ramp, deeply prick, improve Yield and quality, the quality of agricultural-food, make it reach the standard of organic farm products.
Experimental example 1:
In order to verify the fertilizer efficiency of described complex microorganism soil-repairing agent on processing tomato, 2012 Wujiaqu City, Xinjiang agriculture six teacher 101 4 connect 5 bucket 1 agricultures to tomato seedling the situation such as disease-resistant and strong sprout, growing way, output.Be intended to strengthen plant metabolism by this complex microorganism soil-repairing agent, promote photosynthesis and strengthening blade protection film, impel well developed root system, improve and absorb nutrient ability, improve crop yield, improve crop quality; Increase soil beneficial microbe colony, effectively suppress pathogenic bacteria, prevent various disease and pest, reduce pesticide dosage: improvement soil, improve water conservation and the permeability of soil, effectively reduce soil compaction; Promote decomposition of soil organic matter, increase soil fertility, alleviate continuous cropping obstacle; The nutritive substances such as synthesizing amino acid, carbohydrate, VITAMIN, promote seed germination, well developed root system, prematurity, many results.Maturation, the ripening stage shifts to an earlier date more than 3-5 days.
Volume analysis result
From tomato yield analytical results: after application of this complex microorganism soil-repairing agent, tomato mu results strain number adds 60 strains, and improve the resistance of tomato, showing keeps a full stand of seedings to raising certain effect.Tomato per plant number increases by 0.84, single fruit weight increases by 3.25 grams, per unit area yield increases by 8.11 kilograms, volume increase 8.8%, improve effects 324.44 yuan (processing tomato per kilogram is 0.4 yuan), after this complex microorganism soil-repairing agent is used in explanation, mu results strain number, per plant number, single fruit weight and per unit area yield can be improved.
Experimental example 2
This complex microorganism soil-repairing agent has enhancing plant metabolism, promotes photosynthesis and strengthening blade protection film, impels well developed root system, improve and absorb nutrient ability, improve crop yield, improve crop quality.In order to verify this complex microorganism soil-repairing agent result of use on grape, within 2012, connect 2 bucket 5 agricultures to situations such as grape growing way and output Xinjiang Wujiaqu City agriculture six teacher 101 3, test, testing data is as following table.
Grape growth developmental state questionnaire
As can be seen from table, after using this complex microorganism soil-repairing agent, grape leaf look is deepened, and illustrates that crop photosynthesis strengthens, and chlorophyll increases; The thick diameter of uva increases by 2 millimeters and fruit color is deepened, and illustrates that grape grade and quality are in improve.
As can be seen from table, after using this complex microorganism soil-repairing agent, grape mu result strain number increases by 7 strains, single-strain tassel number increases by 0.5, spike weight increases by 20 grams, and per unit area yield increases by 97.88 kilograms, stimulation ratio 17.2%, mu increases economic benefit 489.4 yuan (grape per kilogram is 5 yuan).
Find out from test, after using this complex microorganism soil-repairing agent, grape leaf look is deepened, fruit grain diameter increases, fruit color is deepened, show to enhance photosynthesis of plant, to the effect of being significantly improved of grape grade and quality; And the increase of grape mu result strain number, single-strain tassel number increase, spike weight increase, per unit area yield are increased, household economy benefit can be improved.
Experimental example 3
In order to verify this application of complex microorganism soil-repairing agent on rape, spy seeks car Gu Ying mono-village, town and Tong Zhouqusongzhuan town two places, south of two pier village Daxing District eldest son and carries out simultaneous test, and correlation data is as following table.
Cell production measurement result unit: kg/ha
As can be seen from the above table, test point, Daxing District, the yield increased district volume increase 19.1% for the treatment of zone; Test point, Tongzhou District, the yield increased district volume increase 23.9% for the treatment of zone.
Rape plant height measurement result unit: cm
As can be seen from the above table, test point, Daxing District, the plant height for the treatment of zone increases by 7.7% than check plot; Test point, Tongzhou District, the plant height for the treatment of zone increases by 9.8% than check plot.
Test-results shows: this complex microorganism soil-repairing agent has significant effect to increase yield of rape.
In sum, these are only preferred embodiment of the present invention, be not intended to limit protection scope of the present invention.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. a complex microorganism soil-repairing agent, is characterized in that, is to be mixed by the zymocyte liquid of photosynthetic bacterium, subtilis, plant lactobacillus, yeast and enterococcus faecalis; The volume percent of each zymocyte liquid is: photosynthetic bacterium 5-10%, subtilis 20-25%, plant lactobacillus 20-25%, yeast 20-25%, enterococcus faecalis 20-25%.
2. complex microorganism soil-repairing agent according to claim 1, is characterized in that, described photosynthetic bacterium is preferably the red false first spore bacterium in marsh; Described yeast is preferably yeast saccharomyces cerevisiae.
3. a preparation method for complex microorganism soil-repairing agent described in claim 1 or 2, adopts and symbol strain bacterium is carried out composite method preparation after pure culture in without the fermentor tank of miscellaneous bacteria respectively, the method cultivation that the pure culture process of every strain bacterium is all expanded by secondary; The method comprises the steps:
(1) respectively the mother of photosynthetic bacterium, subtilis, yeast, plant lactobacillus and enterococcus faecalis is planted and be inoculated in one-level and cultivate and very carry out one-level cultivation, obtain first class inoculum;
(2) respectively the first class inoculum of photosynthetic bacterium, subtilis, yeast, plant lactobacillus and enterococcus faecalis is inoculated in secondary to cultivate and more carry out secondary cultivation, obtains zymocyte liquid;
(3) symbol zymocyte liquid is mixed in proportion.
4. the preparation method of complex microorganism soil-repairing agent according to claim 3, is characterized in that, when in described step (1), photosynthetic bacterium one-level is cultivated, substratum is KH
2pO
41.0g, CaCl0.1g, NaHCO
33.0g, sodium acetate 1.0g, MgCl
20.5g, NH
4cl1.0g, NaCl1.0g, trace element solution 1.0ml, vitamin solution 1.0ml, sodium succinate 1.0g, yeast extract 0.5g, peptone 0.5g, distilled water 1.0L, inoculum size is 1.5-5%,
Cultivating pH value is 6.8, and culture temperature is 30 DEG C, and intensity of illumination is 3500-4500 lux, and incubation time is 5-8 days;
When subtilis one-level is cultivated, substratum is peptone 10.0g, beef leaching thing 3.0g, NaCl5.0g, distilled water 1.0L, and inoculum size is 1.5-5%, and cultivating pH value is 7.0, and culture temperature is 37 DEG C, and incubation time is 1-2 days;
When yeast one-level is cultivated, substratum is glucose 10.0g, peptone 10.0g, yeast extract 5g, agar 20g, distilled water 1.0L, and inoculum size is 1.5-5%, and cultivation pH value is 5-7, and culture temperature is 25-28 DEG C, and incubation time is 1-2 days;
When plant lactobacillus one-level is cultivated, substratum is glucose 5.0g, casein peptone 10.0g, extractum carnis 10.0g, yeast powder 5.0g, sodium acetate 5.0g, dibasic ammonium citrate 2.0g, tween 80 1.0g, K
2hPO
42.0g, MgSO
47H
2o0.2g, MnSO
4h
2o0.05g, CaCO
320.0g, agar 15.0g, distilled water 1.0L, inoculum size is 1.5-5%, and cultivating pH value is 6.8, and culture temperature is 30 DEG C, and incubation time is 3-5 days;
When enterococcus faecalis one-level is cultivated, substratum is glucose 5.0g, casein peptone 10.0g, extractum carnis 10.0g, yeast powder 5.0g, sodium acetate 5.0g, dibasic ammonium citrate 2.0g, tween 80 1.0g, K
2hPO
42.0g, MgSO
47H
2o0.2g, MnSO
4h
2o0.05g, CaCO
320.0g, agar 15.0g, distilled water 1.0L, inoculum size is 1.5-5%, and cultivating pH value is 6.8, and culture temperature is 30 DEG C, and incubation time is 3-5 days.
5. the preparation method of complex microorganism soil-repairing agent according to claim 4, it is characterized in that, in step (2), photosynthetic bacterium secondary is cultivated as intermittent type aerated culture, substratum is identical with one-level, inoculum size is 10%-15%, and cultivating pH value is 6.8, and culture temperature is 30 DEG C, intensity of illumination is 3000-4000 lux, and incubation time is 6-8 days;
Subtilis secondary is cultivated and is preferably aerated culture, and substratum is identical with one-level, and inoculum size is 10%-15%, and cultivating pH value is 7.0, and culture temperature is 37 DEG C, and incubation time is 1-2 days;
Yeast secondary is cultivated and is preferably aerated culture, and substratum is identical with one-level, and inoculum size is 10%-15%, and cultivation pH value is 5-7, and culture temperature is 25-28 DEG C, and incubation time is 1-2 days;
Plant lactobacillus secondary is cultivated and is preferably intermittent type aerated culture, and when secondary is cultivated, substratum is identical with one-level, and inoculum size is 10%-15%, and cultivating pH value is 6.8, and culture temperature is 30 DEG C, and incubation time is 5-7 days;
Enterococcus faecalis secondary is cultivated and is preferably intermittent type aerated culture, and when secondary is cultivated, substratum is identical with one-level, and inoculum size is 10%-15%, and cultivating pH value is 6.8, and culture temperature is 30 DEG C, and incubation time is 5-7 days.
6. the application of complex microorganism soil-repairing agent according to claim 1 in rehabilitating soil.
7. the application of complex microorganism soil-repairing agent according to claim 1 in tomato, grape, rapeseed cultivation.
8. a using method for complex microorganism soil-repairing agent according to claim 1, it is characterized in that foliage-spray and fill with root, consumption is 1-2kg/ mu.
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| CN114874043A (en) * | 2022-03-29 | 2022-08-09 | 北京中莲大地生物科技有限公司 | Compound bio-organic fertilizer for improving saline-alkali soil and preparation method thereof |
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