[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

ATE500323T1 - Subtilisin-variante - Google Patents

Subtilisin-variante

Info

Publication number
ATE500323T1
ATE500323T1 AT01923084T AT01923084T ATE500323T1 AT E500323 T1 ATE500323 T1 AT E500323T1 AT 01923084 T AT01923084 T AT 01923084T AT 01923084 T AT01923084 T AT 01923084T AT E500323 T1 ATE500323 T1 AT E500323T1
Authority
AT
Austria
Prior art keywords
homologues
new
subtilisin variant
proteins
subtilisin
Prior art date
Application number
AT01923084T
Other languages
English (en)
Inventor
Jon Ness
Mark Welch
Lorraine Giver
Joel Cherry
Torben Borchert
Willem Stemmer
Jeremy Minshull
Original Assignee
Maxygen Inc
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxygen Inc, Novozymes As filed Critical Maxygen Inc
Application granted granted Critical
Publication of ATE500323T1 publication Critical patent/ATE500323T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/6408Serine endopeptidases (3.4.21)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT01923084T 2000-04-03 2001-04-02 Subtilisin-variante ATE500323T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19414300P 2000-04-03 2000-04-03
PCT/US2001/010781 WO2001075087A2 (en) 2000-04-03 2001-04-02 Subtilisin variants

Publications (1)

Publication Number Publication Date
ATE500323T1 true ATE500323T1 (de) 2011-03-15

Family

ID=22716459

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01923084T ATE500323T1 (de) 2000-04-03 2001-04-02 Subtilisin-variante

Country Status (8)

Country Link
US (4) US6902922B2 (de)
EP (1) EP1287123B1 (de)
JP (1) JP2004508011A (de)
AT (1) ATE500323T1 (de)
AU (4) AU4981101A (de)
CA (1) CA2405063C (de)
DE (1) DE60144145D1 (de)
WO (1) WO2001075087A2 (de)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
DE60144145D1 (de) * 2000-04-03 2011-04-14 Maxygen Inc Subtilisin-variante
EP1280919A2 (de) * 2000-04-28 2003-02-05 Novozymes A/S Varianten eines lipolytischen enzyms
US7109016B2 (en) * 2000-08-21 2006-09-19 Novozymes A/S Subtilase enzymes
AU758744B2 (en) * 2000-10-31 2003-03-27 F. Hoffmann-La Roche Ag Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain
DE10121463A1 (de) * 2001-05-02 2003-02-27 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DK200101090A (da) 2001-07-12 2001-08-16 Novozymes As Subtilase variants
DE10162727A1 (de) * 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
WO2003062380A2 (en) * 2002-01-16 2003-07-31 Genencor International, Inc. Multiply-substituted protease variants
US7306937B2 (en) * 2002-01-16 2007-12-11 Genencor International, Inc. Multiply-substituted protease variants
US7888093B2 (en) 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
EP1623029B1 (de) * 2003-05-07 2008-09-03 Novozymes A/S Enzymvarianten von subtilisin (subtilasen)
US9695251B2 (en) 2003-10-31 2017-07-04 The Regents Of The University Of California Activatable cell penetrating peptides with quenched fluorophores
CN1969042B (zh) * 2004-04-02 2014-09-24 诺维信公司 具有改变的免疫原性的枯草杆菌酶变体
US20090060933A1 (en) * 2004-06-14 2009-03-05 Estell David A Proteases producing an altered immunogenic response and methods of making and using the same
CN101460618A (zh) 2006-04-20 2009-06-17 诺维信公司 对蛋渍具有改进的洗涤性能的洒维奈斯变体
US7802883B2 (en) * 2007-12-20 2010-09-28 Johnson & Johnson Vision Care, Inc. Cosmetic contact lenses having a sparkle effect
JP2011524166A (ja) * 2008-06-06 2011-09-01 ダニスコ・ユーエス・インク 変異体微生物プロテアーゼを含む組成物及び方法
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
DK2285958T3 (en) 2008-06-13 2016-03-29 Codexis Inc Method for synthesizing polynucleotides
DK3301208T3 (da) 2009-09-18 2020-12-14 Codexis Inc Reduceret codon-mutagenese
US20110082055A1 (en) * 2009-09-18 2011-04-07 Codexis, Inc. Reduced codon mutagenesis
JP2016523552A (ja) 2013-07-10 2016-08-12 ノバルティス アーゲー 多重プロテアーゼ欠損糸状菌細胞及びその使用方法
US10513724B2 (en) 2014-07-21 2019-12-24 Glykos Finland Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
SG11201808957UA (en) 2016-05-05 2018-11-29 Codexis Inc Penicillin-g acylases
EP3878967A3 (de) 2017-02-03 2021-12-08 Codexis, Inc. Manipulierte glycosyltransferasen und verfahren zur steviol-glycosid-glucosylierung
BR112019016634A2 (pt) 2017-02-13 2020-04-07 Codexis Inc polipeptídeos, sequência de polinucleotídeos modificados por engenharia, vetor de expressão, célula hospedeira, métodos para produzir um polipeptídeo de fenilalanina amônia liase modificado por engenharia em uma célula hospedeira e para tratar e/ou prevenir os sintomas de fenilcetonúria em um indivíduo, composição, e, uso
US11021729B2 (en) 2017-04-27 2021-06-01 Codexis, Inc. Ketoreductase polypeptides and polynucleotides
HUE055411T2 (hu) 2017-05-08 2021-11-29 Codexis Inc Génmódosított ligáz variánsok
EP3638688A4 (de) 2017-06-14 2021-07-07 Codexis, Inc. Manipulierte transaminase-polypeptide für industrielle biokatalyse
WO2019005337A1 (en) 2017-06-27 2019-01-03 Codexis, Inc. PENICILLIN G ACYLASES
US11319531B2 (en) 2017-11-07 2022-05-03 Codexis, Inc. Transglutaminase variants
EP3724325A4 (de) 2017-12-13 2021-11-24 Codexis, Inc. Carboxyesterase-polypeptide zur amidkopplung
US10900055B2 (en) 2018-06-12 2021-01-26 Codexis, Inc. Engineered tyrosine ammonia lyase
MX2021000323A (es) 2018-07-09 2021-03-25 Codexis Inc Enzimas de variante de pantotenato quinasa modificadas geneticamente.
KR20210031933A (ko) 2018-07-09 2021-03-23 코덱시스, 인코포레이티드 조작된 포스포펜토뮤타제 변이체 효소
US11162105B2 (en) 2018-07-09 2021-11-02 Codexis, Inc. Engineered purine nucleoside phosphorylase variant enzymes
CA3103721A1 (en) 2018-07-09 2020-01-16 Codexis, Inc. Engineered deoxyribose-phosphate aldolases
JP7491587B2 (ja) 2018-07-09 2024-05-28 コデクシス, インコーポレイテッド 操作されたガラクトースオキシダーゼ改変体酵素
WO2020013951A1 (en) 2018-07-12 2020-01-16 Codexis, Inc. Engineered phenylalanine ammonia lyase polypeptides
EP3830106A4 (de) 2018-07-30 2022-07-06 Codexis, Inc. Manipulierte glycosyltransferasen und verfahren zur steviol-glycosid-glucosylierung
CA3116590A1 (en) 2018-10-29 2020-05-07 Codexis, Inc. Engineered dna polymerase variants
EP3893860A4 (de) 2018-12-14 2023-03-29 Codexis, Inc. Manipulierte tyrosin-ammoniak-lyase
JP7245676B2 (ja) * 2019-03-11 2023-03-24 花王株式会社 変異プロテアーゼ
KR20220106738A (ko) 2019-08-30 2022-07-29 코덱시스, 인코포레이티드 조작된 리파제 변이체
WO2021050348A1 (en) 2019-09-12 2021-03-18 Codexis, Inc. Peroxidase activity towards 10-acetyl-3,7-dihydroxyphenoxazine
AU2020408066A1 (en) 2019-12-20 2022-06-30 Codexis, Inc. Engineered acid alpha-glucosidase variants
US11788071B2 (en) 2020-04-10 2023-10-17 Codexis, Inc. Engineered transaminase polypeptides
WO2022047264A1 (en) 2020-08-28 2022-03-03 Codexis, Inc. Engineered amylase variants
CN117098847A (zh) 2020-08-28 2023-11-21 科德克希思公司 工程化蛋白酶变体
JP2023542006A (ja) * 2020-09-21 2023-10-04 バイエル クロップサイエンス エルピー 新規なセリンプロテアーゼ
US11898174B2 (en) 2020-12-18 2024-02-13 Codexis, Inc. Engineered uridine phosphorylase variant enzymes
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
EP4314261A1 (de) 2021-04-02 2024-02-07 Codexis, Inc. Manipulierte enzyme mit acetatkinasevarianten
EP4314262A1 (de) 2021-04-02 2024-02-07 Codexis, Inc. Manipulierte guanylatkinase-variantenenzyme
WO2022212832A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered cyclic gmp-amp synthase (cgas) variant enzymes
CA3214972A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered adenylate kinase variant enzymes
WO2022246126A1 (en) 2021-05-21 2022-11-24 Codexis, Inc. Engineered methionine gamma lyase variants
EP4416278A1 (de) 2021-10-15 2024-08-21 Codexis, Inc. Manipulierte dna-polymerasevarianten

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US49827A (en) * 1865-09-05 Improvement in machines for making cement pipe
PT86864B (pt) * 1987-02-27 1992-05-29 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
ATE168130T1 (de) * 1991-05-01 1998-07-15 Novo Nordisk As Stabilisierte enzyme und waschmittelzusammensetzungen
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5316935A (en) 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
EP0717778B1 (de) * 1992-07-17 2007-10-17 Genencor International, Inc. Hochalkalische serinproteasen
DE69331907T2 (de) * 1992-09-24 2002-09-05 Genencor International, Inc. Neue alkalische proteasen enthaltende reinigungsmittelzusammensetzungen
WO1995010591A1 (en) * 1993-10-14 1995-04-20 The Procter & Gamble Company Protease-containing cleaning compositions
CN1177921C (zh) * 1993-10-14 2004-12-01 普罗格特-甘布尔公司 含有蛋白酶的漂白组合物
US5972042A (en) 1995-12-22 1999-10-26 Novo Nordisk A/S Method for dyeing a material with a dyeing system which contains an enzymatic oxidizing agent
BR9712878A (pt) * 1996-11-04 2000-02-01 Novo Nordisk As Variante de enzima subtilase, processos para a identificação de uma variante de protease apresentando estabilidade autoproteolìtica e paraq a produção de uma enzima subtilase mutante e de uma variante de subtilase, sequência de dna, vetor, célula hospedeira microbiana, composição e uso de uma variante de subtilase.
MA24811A1 (fr) * 1997-10-23 1999-12-31 Procter & Gamble Compositions de lavage contenant des variantes de proteases multisubstituees
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
DE60144145D1 (de) * 2000-04-03 2011-04-14 Maxygen Inc Subtilisin-variante
DK200101090A (da) * 2001-07-12 2001-08-16 Novozymes As Subtilase variants
DE10162728A1 (de) * 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease

Also Published As

Publication number Publication date
US7220566B2 (en) 2007-05-22
CA2405063C (en) 2013-06-04
WO2001075087A3 (en) 2002-12-19
US20030049827A1 (en) 2003-03-13
AU2006208427A1 (en) 2006-09-28
EP1287123B1 (de) 2011-03-02
WO2001075087A8 (en) 2001-12-27
DE60144145D1 (de) 2011-04-14
AU2001249811B2 (en) 2006-06-08
JP2004508011A (ja) 2004-03-18
US7605115B2 (en) 2009-10-20
WO2001075087A2 (en) 2001-10-11
AU2009251169A1 (en) 2010-01-21
US20100105599A1 (en) 2010-04-29
EP1287123A2 (de) 2003-03-05
AU2006208427B2 (en) 2009-10-01
CA2405063A1 (en) 2001-10-11
US20070270574A1 (en) 2007-11-22
US6902922B2 (en) 2005-06-07
US20040203130A1 (en) 2004-10-14
AU4981101A (en) 2001-10-15

Similar Documents

Publication Publication Date Title
DE60144145D1 (de) Subtilisin-variante
WO2002006457A3 (en) Novel lipase genes
DK1210411T3 (da) Sammensætninger og fremgangsmåder til forbedret celledyrkning
DE60127441D1 (de) Dipeptidylpeptidasen
DE69942459D1 (de) Isolierte nukleinsäuremoleküle die t-zellen induzierbare faktoren kodieren (tif), kodierte proteine und ihre anwendung
AR006188A1 (es) "composicion farmaceutica para inhibir la actividad de serinproteasa, metodo in vitro para inhibir la actividad de serinproteasa y metodo para preparar dicha composicion".
DE60037450D1 (de) Funf-helix protein
WO2002031134A3 (en) Novel serine protease genes related to dppiv
ATE400656T1 (de) Mini-dystrophin nukleinsäure- und peptidsequenzen
DE50112802D1 (de) Peptide, deren herstellung und verwendung zur bindung von immunglobulinen
DK1131444T3 (da) Polypeptider med lysophospholipase aktivitet og nucleinsyre som koder for disse
DK0981630T3 (da) Polypeptider med prolyldipeptidylaminopeptidaseaktivitet og nukleinsyrer, der koder for samme
ATE474926T1 (de) Verwendung von clya hemolysin fur ausscheidung von fusionprotein
WO2001025438A3 (en) Ifn-alpha homologues
ATE439441T1 (de) Veränderte, fluoreszierende proteine
DK1082423T3 (da) Cyclin E2-gener og -proteiner
DK0939769T3 (da) Vertebrat-Smoothened-proteiner
ATE309370T1 (de) Retinoid-metabolisierendes protein
SE9602822D0 (sv) New receptor
AU2001238064A1 (en) 18477, a human protein kinase and uses therefor
WO2001064912A3 (en) Triazine degrading enzymes
WO2002062964A3 (en) Ap1 amine oxidase variants
WO2001040257A3 (en) Modified il-12 subunits and nucleic acids encoding them
WO2001096593A3 (en) Novel motor proteins and methods for their use
WO2002012541A3 (en) Novel motor proteins and methods for their use

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties