KR20210031933A

KR20210031933A - 조작된 포스포펜토뮤타제 변이체 효소

Info

Publication number: KR20210031933A
Application number: KR1020217003849A
Authority: KR
Inventors: 스콧 제이 노빅; 시앙 이; 닉키 델라스; 오스카 알비조; 조바나 나조르; 다 두안; 베스나 미첼; 조나단 브룸; 산토쉬 시바라막리시난; 난드히타 수브라마니안; 제프리 씨 무어; 마크 허프만; 어거스티나 로드리게즈-그라닐로; 딥탁 버마; 그랜트 머피; 니콜라스 엠 마샬; 제이 러셀; 키스 에이 캐나다
Original assignee: 코덱시스, 인코포레이티드
Priority date: 2018-07-09
Filing date: 2019-07-02
Publication date: 2021-03-23
Also published as: US11795445B2; JP2021530981A; SG11202012109UA; US20200010823A1; AU2019302422A1; US20210261940A1; IL279880A; CA3108728A1; US11034948B2; JP2024040366A; CN112601821A; AU2019302422B2; JP7493248B2; US20240060064A1; WO2020014046A1; MX2021000322A; EP3821026A4; EP3821026A1

Abstract

본 발명은 조작된 포스포펜토뮤타제(PPM) 효소, PPM 활성을 갖는 폴리펩타이드, 및 이들 효소를 코딩하는 폴리뉴클레오타이드뿐만 아니라, 이들 폴리뉴클레오타이드 및 폴리펩타이드를 포함하는 벡터 및 숙주 세포를 제공한다. PPM 효소를 생산하는 방법도 또한 제공된다. 본 발명은 추가로 PPM 효소를 포함하는 조성물 및 조작된 PPM 효소를 사용하는 방법을 제공한다. 본 발명은 약제학적 화합물의 생산에서 특별한 용도를 제시한다.

Description

조작된 포스포펜토뮤타제 변이체 효소

본 출원은 2018년 7월 9일 출원된 미국 특허 가출원 제62/695,491호에 기초한 우선권을 주장하고, 상기 출원은 모든 목적을 위해 그 전체가 참조로 포함된다.

발명의 분야

서열 목록, 표 또는 컴퓨터 프로그램에 대한 언급

서열 목록의 공식 사본은 EFS-Web을 통해 ASCII 형식의 텍스트 파일로 본 명세서와 동시에 제출되고, 파일 이름은 "CX2-171WO2_ST25.txt"이고, 그 작성일은 2019년 7월 2일이며, 파일 크기는 2,904 킬로바이트이다. EFS-Web을 통해 제출된 서열 목록은 명세서의 일부이며, 그 전체가 본 명세서에 참조로 포함된다.

인간 면역 결핍 바이러스(HIV)로 지정된 레트로바이러스는 이환된 개체의 면역계의 점진적인 파괴 및 중추 및 말초 신경계의 퇴화를 수반하는 복합 질환인 후천성 면역 결핍 증후군(AIDS)의 병인체이다. 레트로바이러스 복제의 일반적인 특징은 바이러스 복제에 필요한 HIV 서열의 DNA 사본을 생성하기 위해 바이러스에 의해 코딩되는 역전사 효소에 의한 바이러스 RNA 게놈의 역전사이다. MK-8591과 같은 일부 화합물은 역전사 효소 억제제로 알려져 있으며, AIDS 및 유사한 질환의 치료에 유용한 것으로 밝혀졌다. HIV 역전사 효소를 억제하는 것으로 알려진 일부 화합물이 있지만, 상기 효소를 억제하는데 보다 효과적이고 따라서 AIDS의 효과를 개선하는 추가의 화합물에 대한 필요성이 관련 기술 분야에 계속 존재한다.

MK-8591(Merck)과 같은 뉴클레오사이드 유사체는 DNA 합성에 사용되는 천연 뉴클레오사이드와의 유사성 때문에 HIV의 역전사 효소에 대한 효과적인 억제제이다. 역전사 효소에 의한 이러한 유사체의 결합은 역전사 효소의 점진적 특성을 억제함으로써 DNA 합성을 정지시킨다. 효소의 정지는 DNA 분자의 조기 종결을 초래하여 상기 분자를 효과적이지 못하도록 만든다. 그러나, 표준 화학 합성 기술에 의한 뉴클레오사이드 유사체의 생산은 이들의 화학적 복잡성으로 인해 문제를 제기할 수 있다.

발명의 요약

본 발명은 서열 번호 2, 4, 118, 266, 420, 562, 656, 790 및/또는 846에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열, 또는 이의 기능적 단편을 포함하는 조작된 포스포펜토뮤타제을 제공하고, 여기서 상기 조작된 포스포펜토뮤타제는 상기 폴리펩타이드 서열에 적어도 하나의 치환 또는 치환 세트를 포함하는 폴리펩타이드를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 2, 4, 118, 266, 420, 562, 656, 790, 및/또는 846을 기준으로 넘버링된다. 일부 실시양태에서, 폴리펩타이드 서열은 서열 번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 2를 기준으로 넘버링된다. 일부 실시양태에서, 조작된 포스포펜토뮤타제의 폴리펩타이드 서열은 서열 번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 2를 기준으로 넘버링된다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 2에 대해 적어도 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 2에 대해 적어도 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다.

일부 실시양태에서, 본 발명은 서열 번호 4에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 조작된 포스포펜토뮤타제를 제공하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열은

으로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 70을 기준으로 넘버링된다. 일부 실시양태에서, 본 발명은 서열 번호 4에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 갖는 조작된 포스포펜토뮤타제를 제공하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 4를 기준으로 넘버링된다. 일부 실시양태에서, 본 발명은 서열 번호 4에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 갖는 조작된 포스포펜토뮤타제를 제공하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 4를 기준으로 넘버링된다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 4에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 4에 대해 적어도 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 4에 대해 적어도 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다.

일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 118에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열은

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 118을 기준으로 넘버링된다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 118에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 118을 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 118에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 118을 기준으로 넘버링된다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 118에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 118에 대해 적어도 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 118에 대해 적어도 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다.

일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 266에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는 65/225, 65/225/263, 97, 118, 143/194, 147, 156, 160, 172, 176, 188, 192, 194, 207, 211, 217, 225, 227, 236, 238, 257, 257/261, 261, 263, 306, 및 397로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 266을 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 266에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는 65E/225P, 65E/225P/263V, 97E, 118T, 143A/194G, 147A, 147R, 156W, 160S, 172G, 176L, 188Q, 192T, 194S, 207E, 211A, 217N, 225K, 225P, 227S, 236P, 236T, 238G, 257A, 257I, 257I/261G, 257M, 257S, 257T, 261G, 263G, 306V, 및 397V로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 266을 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 조작된 포스포펜토뮤타제는 서열 번호 266에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 266을 기준으로 넘버링된다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 266에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 266에 대해 적어도 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 266에 대해 적어도 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다.

일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 266에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드를 포함하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 266을 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 266에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 266을 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 266에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 420에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는 12, 94, 118/147/227/238/257/357, 118/151, 118/236/357, 121, 142, 148, 150, 151, 172, 181, 185, 186, 189, 193, 199, 206, 218, 235, 237, 239, 256, 266, 267, 281, 300, 308, 및 383으로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 420을 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 420에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 420을 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 420에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 420을 기준으로 넘버링된다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 420에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 420에 대해 적어도 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 420에 대해 적어도 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다.

일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 562에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는 21, 118, 120, 151, 155, 220, 225 및 357로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 562를 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 562에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 562를 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 562에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는 21W, 118R, 120G, 120L, 120R, 120T, 151L, 151T, 155A, 155S, 220P, 225R, 357E, 및 357Q로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 562를 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 562에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는 E21W, T118R, H120G, H120L, H120R, H120T, V151L, V151T, Q155A, Q155S, K220P, Q225R, H357E, 및 H357Q로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 562를 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 420에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 562를 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 562에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 562를 기준으로 넘버링된다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 562에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 562에 대해 적어도 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 562에 대해 적어도 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다.

일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 656에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는 6, 8, 39, 46, 53, 108, 133, 151, 160, 192, 196, 200, 217, 225, 236, 239, 251, 257, 272, 284, 335, 341, 368, 369, 391, 및 397로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 656을 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 656에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는 6M, 8T, 39Y, 46K, 46M, 46S, 53V, 108T, 133L, 151Q, 160S, 192D, 192T, 196V, 200M, 217V, 225N, 236A, 239G, 239V, 251A, 257L, 257V, 272A, 284V, 335T, 341L, 341V, 368L, 369L, 391I, 및 397L로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 656을 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 656에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는 I6M, V8T, A39Y, G46K, G46M, G46S, L53V, V108T, V133L, V151Q, H160S, E192D, E192T, I196V, E200M, I217V, Q225N, E236A, A239G, A239V, H251A, A257L, A257V, K272A, A284V, L335T, I341L, I341V, V368L, Y369L, L391I, 및 T397L로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 656을 기준으로 넘버링된다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 656에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 656에 대해 적어도 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 656에 대해 적어도 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다.

일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 790에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 790을 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 790에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 790을 기준으로 넘버링된다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 790에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 790에 대해 적어도 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 790에 대해 적어도 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다.

일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 846에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

으로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 846을 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 846에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 846을 기준으로 넘버링된다. 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 846에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함하고, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드는

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 846을 기준으로 넘버링된다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 846에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 846에 대해 적어도 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 846에 대해 적어도 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다.

일부 추가의 실시양태에서, 본 발명은 조작된 포스포펜토뮤타제를 제공하고, 여기서 조작된 포스포펜토뮤타제는 표 4.1, 5.1, 6.1, 7.1, 8.1, 9.1, 10.1, 12.1, 12.2, 13.1, 14.1 및/또는 15.1에 제시된 적어도 하나의 조작된 포스포펜토뮤타제 변이체의 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다.

일부 추가의 실시양태에서, 본 발명은 서열 번호 2, 4, 118, 266, 420, 562, 656, 790 및/또는 846에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 조작된 포스포펜토뮤타제는 서열 번호 4, 118, 266, 420, 562, 656, 790 및/또는 846에 제시된 조작된 포스포펜토뮤타제 변이체를 포함한다.

본 발명은 또한 서열 번호 8-1152의 짝수 번호 서열에 제시된 적어도 하나의 조작된 포스포펜토뮤타제 변이체에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다.

본 발명은 추가로 조작된 포스포펜토뮤타제를 제공하며, 여기서 상기 조작된 포스포펜토뮤타제는 야생형 이. 콜라이(E. coli) 포스포펜토뮤타제에 비해 적어도 하나의 개선된 특성을 포함한다. 일부 실시양태에서, 개선된 특성은 기질에 대한 개선된 활성을 포함한다. 일부 추가의 실시양태에서, 기질은 화합물 2 및/또는 화합물 3을 포함한다. 일부 추가의 실시양태에서, 개선된 특성은 화합물 1 및/또는 화합물 4의 개선된 생산을 포함한다. 일부 추가의 실시양태에서, 기질은 화합물 4를 포함한다. 일부 추가의 실시양태에서, 개선된 특성은 화합물 1 및/또는 화합물 3의 개선된 생산을 포함한다. 또 다른 일부 추가의 실시양태에서, 조작된 포스포펜토뮤타제는 정제된다. 본 발명은 또한 본원에서 제공되는 적어도 하나의 조작된 포스포펜토뮤타제를 포함하는 조성물을 제공한다. 본 발명은 또한 본원에 제공되는 적어도 하나의 조작된 포스포펜토뮤타제를 포함하는 조성물을 제공한다.

본 발명은 또한 본원에서 제공되는 적어도 하나의 조작된 포스포펜토뮤타제를 코딩하는 폴리뉴클레오타이드 서열을 제공한다. 일부 실시양태에서, 적어도 하나의 조작된 포스포펜토뮤타제를 코딩하는 폴리뉴클레오타이드 서열은 서열 번호 1, 3, 117, 265, 419, 561, 655, 789 및/또는 845에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리뉴클레오타이드 서열을 포함한다. 일부 실시양태에서, 적어도 하나의 조작된 포스포펜토뮤타제를 코딩하는 폴리뉴클레오타이드 서열은 서열 번호 1, 3, 117, 265, 419, 561, 655, 789 및/또는 845에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리뉴클레오타이드 서열을 포함하고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리뉴클레오타이드 서열은 하나 이상의 위치에 적어도 하나의 치환을 포함한다. 일부 추가의 실시양태에서, 적어도 하나의 조작된 포스포펜토뮤타제를 코딩하는 폴리뉴클레오타이드 서열은 서열 번호 1, 3, 117, 265, 419, 561, 655, 789 및/또는 845에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리뉴클레오타이드 서열, 또는 그의 기능적 단편을 포함한다. 일부 추가의 실시양태에서, 폴리뉴클레오타이드 서열은 제어 서열에 작동 가능하게 연결된다. 일부 추가의 실시양태에서, 폴리뉴클레오타이드 서열은 코돈 최적화된다. 일부 추가의 실시양태에서, 폴리뉴클레오타이드 서열은 서열 번호 7-1151의 홀수 번호 서열에 제시된 폴리뉴클레오타이드 서열을 포함한다.

본 발명은 또한 본원에서 제공되는 적어도 하나의 폴리뉴클레오타이드 서열을 포함하는 발현 벡터를 제공한다. 본 발명은 추가로 본원에서 제공되는 적어도 하나의 발현 벡터를 포함하는 숙주 세포를 제공한다. 일부 실시양태에서, 본 발명은 본원에서 제공되는 적어도 하나의 폴리뉴클레오타이드 서열을 포함하는 숙주 세포를 제공한다.

본 발명은 또한 숙주 세포에서 조작된 포스포펜토뮤타제를 생산하는 방법을 제공하며, 이 방법은 적어도 하나의 조작된 포스포펜토뮤타제가 생산되도록 적합한 조건 하에서 본원에서 제공되는 숙주 세포를 배양하는 단계를 포함한다. 일부 실시양태에서, 상기 방법은 배양액 및/또는 숙주 세포로부터 적어도 하나의 조작된 포스포펜토뮤타제를 회수하는 단계를 추가로 포함한다. 일부 추가의 실시양태에서, 상기 방법은 상기 적어도 하나의 조작된 포스포펜토뮤타제를 정제하는 단계를 추가로 포함한다.

발명의 설명

본 발명은 조작된 포스포펜토뮤타제(PPM) 효소, PMP 활성을 갖는 폴리펩타이드, 및 이들 효소를 코딩하는 폴리뉴클레오타이드뿐만 아니라, 이들 폴리뉴클레오타이드 및 폴리펩타이드를 포함하는 벡터 및 숙주 세포를 제공한다. PPM 효소를 생산하는 방법도 제공된다. 본 발명은 추가로 PPM 효소를 포함하는 조성물 및 조작된 PPM 효소를 사용하는 방법을 제공한다. 본 발명은 약제학적 화합물의 생산에서 특별한 용도를 제시한다.

달리 정의되지 않는 한, 본원에서 사용되는 모든 기술 및 과학 용어는 일반적으로 본 발명이 속하는 기술 분야에서 통상의 기술을 가진 자에 의해 일반적으로 이해되는 것과 동일한 의미를 갖는다. 일반적으로, 본원에서 사용 명명법 및 아래에서 설명되는 세포 배양, 분자 유전학, 미생물학, 유기 화학, 분석 화학 및 핵산 화학의 실험 절차는 관련 기술 분야에 널리 공지되고 일반적으로 사용되는 것이다. 이러한 기술은 널리 공지되어 있고 관련 기술 분야에서 통상의 기술을 가진 자에게 널리 알려진 많은 교과서 및 참고서에 기재되어 있다. 표준 기술, 또는 이의 변형이 화학적 합성 및 화학적 분석에 사용된다. 상기 및 아래에서 언급되는 모든 특허, 특허 출원, 논문 및 간행물은 본원에서 참고로 명시적으로 포함된다.

본원에서 설명되는 것과 유사하거나 대등한 임의의 적합한 방법 및 물질이 본 발명의 실시에 사용되지만, 일부 방법 및 물질이 본원에서 설명된다. 본 발명은 기재된 특정 방법론, 프로토콜, 및 시약으로 한정되지 않는 것으로 이해되어야 하는데, 이들은 이들이 관련 기술 분야에서 통상의 기술을 가진 자에 의해 사용되는 맥락에 따라 달라질 수 있기 때문이다. 따라서, 바로 아래에 정의된 용어는 본 발명을 전체로서 참조하여 보다 완전하게 설명된다.

전술한 일반적인 설명과 하기의 상세한 설명은 모두 단지 예시적이고 설명하기 위한 것으로서, 본 발명을 제한하는 것이 아닌 것으로 이해되어야 한다. 본원에서 사용되는 섹션 제목은 단지 그 구성을 조직적으로 제시하기 위한 것이며 기술된 주제를 제한하는 것으로 해석되지 않아야 한다. 수치 범위는 범위를 한정하는 수를 포함한다. 따라서, 본원에 개시되는 모든 수치 범위는, 마치 더 좁은 수치 범위가 모두 본원에 명시적으로 기재된 것처럼, 이러한 더 넓은 수치 범위 내에 속하는 모든 더 좁은 수치 범위를 포함하는 것으로 의도된다. 또한, 본원에서 개시되는 모든 최대(또는 최소) 수치 제한은, 마치 더 낮은(또는 더 높은) 수치 제한이 본원에 명시적으로 기재된 것처럼, 모든 더 낮은(또는 더 높은) 수치 한정을 포함하는 것으로 의도된다.

약어 및 정의

유전자에 의해 코딩되는 아미노산에 대해 사용되는 약어는 통상적이고, 다음과 같다: 알라닌(Ala 또는 A), 아르기닌(Are 또는 R), 아스파라긴(Asn 또는 N), 아스파르테이트(Asp 또는 D), 시스테인(Cys 또는 C), 글루타메이트(Glu 또는 E), 글루타민(Gln 또는 Q), 히스티딘(His 또는 H), 이소류신(Ile 또는 I), 류신(Leu 또는 L), 라이신(Lys 또는 K), 메티오닌(Met 또는 M), 페닐알라닌(Phe 또는 F), 프롤린(Pro 또는 P), 세린(Ser 또는 S), 트레오닌(Thr 또는 T), 트립토판(Trp 또는 W), 티로신(Tyr 또는 Y) 및 발린(Val 또는 V).

세 글자 약어가 사용되는 경우, 명시적으로 "L" 또는 "D"가 앞에 나오지 않거나 또는 약어가 사용된 문맥으로부터 명확하지 않는 한, 아미노산은 α-탄소(Cα)에 대해 L- 또는 D-배열일 수 있다. 예를 들어, "Ala"는 α-탄소에 대한 배열을 명시하지 않은 알라닌을 나타내고, "D-Ala" 및 "L-Ala"는 각각 D-알라닌 및 L-알라닌을 나타낸다. 한 글자 약어가 사용되는 경우, 대문자는 α-탄소에 대한 L-배열의 아미노산을 나타내고, 소문자는 α-탄소에 대한 D-배열의 아미노산을 나타낸다. 예를 들어, "A"는 L-알라닌을 나타내고, "a"는 D-알라닌을 나타낸다. 폴리펩타이드 서열이 한 줄의 한 글자 또는 세 글자 약어(또는 이들의 혼합)로 제시될 때, 서열은 통상적인 관례에 따라 아미노(N)에서 카르복시(C) 방향으로 제시된다.

유전자에 의해 코딩되는 뉴클레오사이드에 대해 사용된 약어는 통상적이며, 다음과 같다: 아데노신(A); 구아노신(G); 시티딘(C); 티미딘(T); 및 우리딘(U). 명시적으로 서술되지 않는 한, 약칭된 뉴클레오사이드는 리보뉴클레오사이드 또는 2'-데옥시리보뉴클레오사이드일 수 있다. 뉴클레오사이드는 개별적인 기준으로 또는 종합적인 기준으로 리보뉴클레오사이드 또는 2'-데옥시리보뉴클레오사이드인 것으로 특정될 수 있다. 핵산 서열이 한 줄의 한 글자 약어로 제시될 때, 서열은 통상적인 관례에 따라 5'에서 3' 방향으로 제시되고, 포스페이트는 표시되지 않는다.

본 발명과 관련하여, 본원의 설명에 사용되는 기술 및 과학 용어는, 달리 명시적으로 정의되지 않는 한, 관련 기술 분야에서 통상의 기술을 가진 자에 의해 통상적으로 이해되는 의미를 가질 것이다. 따라서, 하기 용어는 하기 의미를 갖는 것으로 의도된다.

본원에 사용되는 바와 같이, 단수 형태 "a", "an" 및 "the"는 문맥이 달리 명확히 나타내지 않는 한, 복수의 대상을 포함한다. 따라서, 예를 들어, "폴리펩타이드"에 대한 언급은 둘 이상의 폴리펩타이드를 포함한다.

이와 유사하게, "포함하다", "포함하는", "포괄하다", 및 " 포괄하는"은 상호 교환 가능하며, 제한하고자 하는 것은 아니다. 따라서, 본원에서 사용되는 바와 같이, 용어 "포함하는" 및 그의 동족어는 포괄적인 의미로 사용된다(즉, 용어 "포괄하는" 및 그의 상응하는 동족어와 동등함).

다양한 실시양태의 설명이 용어 "포함하는"을 사용하는 경우, 관련 기술 분야에서 통상의 기술을 가진 자는 일부 특정 경우에, 실시양태가 표현 "본질적으로 이루어지는" 또는 "이루어지는"을 사용하여 대안적으로 설명될 수 있음을 이해할 것임이 추가로 이해되어야 한다.

본원에서 사용되는 바와 같이, 용어 "약"은 특정 값에 대한 허용 가능한 오차를 의미한다. 일부 경우에, "약"은 주어진 값 범위의 0.05%, 0.5%, 1.0%, 또는 2.0% 이내를 의미한다. 일부 경우에, "약"은 주어진 값의 1, 2, 3, 또는 4 표준 편차 이내를 의미한다.

본원에서 사용되는 바와 같이, "EC" 번호는 생화학 및 분자 생물학의 국제 연합의 명명 위원회(NC-IUBMB)의 효소 명명법을 지칭한다. IUBMB 생화학 분류는 효소가 촉매작용을 하는 화학적 반응에 기초한, 효소에 대한 수치적 분류 시스템이다.

본원에서 사용되는 바와 같이, "ATCC"는 그의 생체 시료 저장(biorepository) 수집물이 유전자 및 균주를 포함하는 아메리칸 타입 컬쳐 컬렉션(American Type Culture Collection)을 지칭한다.

본원에서 사용되는 바와 같이, "NCBI"는 미국 국립 생물 정보 센터(National Center for Biological Information) 및 그 안에 제공된 서열 데이터베이스를 지칭한다.

본원에서 사용되는 바와 같이, "포스포펜토뮤타제"("PPM") 효소는 리보스 1-포스페이트의 리보스 5-포스페이트 및 관련 화합물, 예를 들어 데옥시리보스 포스페이트 및 리보스 포스페이트 및 데옥시리보스 포스페이트의 유사체로의 가역적 이성질체화를 촉매하는 효소이다.

본원에서 사용되는 바와 같이, "퓨린 뉴클레오사이드 포스포릴라제"("PNP") 효소는 퓨린 리보뉴클레오사이드 및 관련 화합물(예를 들어, 데옥시리보뉴클레오사이드 및 리보뉴클레오사이드 및 데옥시리보뉴클레오사이드의 유사체)의 유리 퓨린 염기 및 리보스-1-포스페이트(및 이의 유사체)로의 가역적 인산화를 촉매하는 효소이다.

"단백질", "폴리펩타이드" 및 "펩타이드"는, 길이 또는 번역후 변형(예를 들어, 글리코실화 또는 인산화)과 관계없이, 아미드 결합에 의해 공유 결합된 적어도 2개의 아미노산의 중합체를 지칭하기 위해 본원에서 상호 교환 가능하게 사용된다. 이 정의에는 D- 및 L-아미노산, 및 D-아미노산과 L-아미노산의 혼합물뿐만 아니라, D- 및 L-아미노산을 포함하는 중합체, 및 D-아미노산과 L-아미노산의 혼합물이 포함된다.

"아미노산"은 이들의 통상적으로 공지된 세 글자 기호에 의해 또는 IUPAC-IUB 생화학적 명명 심의회(Biochemical Nomenclature Commission)에 의해 권고된 한 글자 기호에 의해 본원에서 언급된다. 마찬가지로, 뉴클레오타이드는 이들의 일반적으로 허용되는 단일 글자 코드에 의해 언급될 수 있다.

본원에서 사용되는 바와 같이, "친수성 아미노산 또는 잔기"는 문헌 [Eisenberg et al., J. Mol. Biol., 179:125-142 [1984]]의 표준화된 합의된 소수성 척도에 따라 0 미만의 소수성을 나타내는 측쇄를 갖는 아미노산 또는 잔기를 의미한다. 유전자에 의해 코딩되는 친수성 아미노산에는 L-Thr(T), L-Ser(S), L-His(H), L-Glu(E), L-Asn(N), L-Gln(Q), L-Asp(D), L-Lys(K) 및 L-Arg(R)이 포함된다.

본원에서 사용되는 바와 같이, "산성 아미노산 또는 잔기"는 아미노산이 펩타이드 또는 폴리펩타이드에 포함될 때 약 6 미만의 pK 값을 나타내는 측쇄를 갖는 친수성 아미노산 또는 잔기를 지칭한다. 산성 아미노산은 통상적으로 수소 이온의 손실로 인해 생리학적 pH에서 음 하전된 측쇄를 갖는다. 유전자에 의해 코딩되는 산성 아미노산에는 L-Glu(E) 및 L-Asp(D)가 포함된다.

본원에서 사용되는 바와 같이, "염기성 아미노산 또는 잔기"는 아미노산이 펩타이드 또는 폴리펩타이드에 포함될 때 약 6 초과의 pK 값을 나타내는 측쇄를 갖는 친수성 아미노산 또는 잔기를 지칭한다. 염기성 아미노산은 통상적으로 히드로늄 이온과의 회합으로 인해 생리학적 pH에서 양 하전된 측쇄를 갖는다. 유전자에 의해 코딩되는 염기성 아미노산에는 L-Arg(R) 및 L-Lys(K)가 포함된다.

본원에서 사용되는 바와 같이, "극성 아미노산 또는 잔기"는 생리학적 pH에서 하전되어 있지 않지만 2개의 원자에 의해 공유된 한 쌍의 전자가 원자 중 1개에 의해 더욱 가까이 유지된 적어도 1개의 결합을 갖는 측쇄를 갖는 친수성 아미노산 또는 잔기를 지칭한다. 유전자에 의해 코딩되는 극성 아미노산에는 L-Asn(N), L-Gln(Q), L-Ser(S) 및 L-Thr(T)이 포함된다.

본원에서 사용되는 바와 같이, "소수성 아미노산 또는 잔기"는 문헌 [Eisenberg et al., J. Mol. Biol., 179:125-142 [1984]]의 표준화된 합의된 소수성 척도에 따라 0 초과의 소수성을 나타내는 측쇄를 갖는 아미노산 또는 잔기를 지칭한다. 유전자에 의해 코딩되는 소수성 아미노산에는 L-Pro(P), L-Ile(I), L-Phe(F), L-Val(V), L-Leu(L), L-Trp(W), L-Met(M), L-Ala(A) 및 L-Tyr(Y)이 포함된다.

본원에서 사용되는 바와 같이, "방향족 아미노산 또는 잔기"는 적어도 1개의 방향족 또는 헤테로방향족 고리를 포함하는 측쇄를 갖는 친수성 또는 소수성 아미노산 또는 잔기를 지칭한다. 유전자에 의해 코딩되는 방향족 아미노산은 L-Phe(F), L-Tyr(Y) 및 L-Trp(W)를 포함한다. 그의 헤테로방향족 질소 원자 L-His(H)의 pKa로 인해, 이는 때때로 염기성 잔기로서 분류되거나, 이의 측쇄가 헤테로방향족 고리를 포함하기 때문에 방향족 잔기로서 분류되지만, 본원에서는 히스티딘을 친수성 잔기 또는 "구속형(constrained) 잔기"로서 분류한다(하기 참조).

본원에서 사용되는 바와 같이, "구속형 아미노산 또는 잔기"는 구속형 기하형태를 갖는 아미노산 또는 잔기를 지칭한다. 본원에서, 구속형 잔기는 L-pro(P) 및 L-His(H)을 포함한다. 히스티딘은 구속형 기하형태를 갖는데, 이는 이것이 비교적 작은 이미다졸 고리를 갖기 때문이다. 프롤린은 구속형 기하형태를 가지는데, 이는 그것이 또한 5원 고리를 갖기 때문이다.

본원에서 사용되는 바와 같이, "비극성 아미노산 또는 잔기"는 생리학적 pH에서 하전되어 있지 않고 2개 원자에 의해 공유된 한 쌍의 전자가 일반적으로 2개 원자 각각에 의해 동일하게 유지된 결합을 갖는 측쇄를 갖는(즉, 측쇄는 극성이 아님) 소수성 아미노산 또는 잔기를 지칭한다. 유전자에 의해 코딩되는 비극성 아미노산에는 L-Gly(G), L-Leu(L), L-Val(V), L-Ile(I), L-Met(M) 및 L-Ala(A)이 포함된다.

본원에서 사용되는 바와 같이, "지방족 아미노산 또는 잔기"는 지방족 탄화수소 측쇄를 갖는 소수성 아미노산 또는 잔기를 지칭한다. 유전자에 의해 코딩되는 지방족 아미노산에는 L-Ala(A), L-Val(V), L-Leu(L) 및 L-Ile(I)이 포함된다. 시스테인(또는 "L-Cys" 또는 "[C]")은 이것이 다른 L-Cys(C) 아미노산 또는 다른 술파닐- 또는 술프히드릴 함유 아미노산과 디술파이드 다리를 형성할 수 있다는 점에서 특이하다는 사실에 유의해야 한다. "시스테인 유사 잔기"는 시스테인, 및 디술파이드 다리의 형성에 이용할 수 있는 술프히드릴 모이어티를 함유하는 다른 아미노산을 포함한다. L-Cys(C)(및 -SH 함유 측쇄를 갖는 다른 아미노산)이 환원된 유리 -SH 또는 산화된 디술파이드 다리 형태의 펩타이드에 존재하는 능력은, L-Cys(C)가 펩타이드에 대한 전체적인 소수성 또는 친수성 특성에 기여하는지의 여부에 영향을 미친다. L-Cys(C)이 아이젠베르크(Eisenberg)의 표준화된 합의된 소수성 척도(Eisenberg et al., 1984, 상기 문헌)에 따라 0.29의 소수도를 나타내긴 하지만, 본 개시내용의 목적을 위해 L-Cys(C)은 그의 자체의 특유한 기로 분류됨을 이해하여야 한다.

본원에서 사용되는 바와 같이, "소형 아미노산 또는 잔기"는 총 3개 이하의 탄소 및/또는 헤테로원자(α-탄소 및 수소 제외)로 이루어지는 측쇄를 갖는 아미노산 또는 잔기를 지칭한다. 소형 아미노산 또는 잔기는 상기 정의에 따라 지방족, 비극성, 극성 또는 산성 소형 아미노산 또는 잔기로 추가로 분류될 수 있다. 유전자에 의해 코딩되는 소형 아미노산은 L-Ala(A), L-Val(V), L-Cys(C), L-Asn(N), L-Ser(S), L-Thr(T) 및 L-Asp(D)을 포함한다.

본원에서 사용되는 바와 같이, "하이드록실 함유 아미노산 또는 잔기"는 하이드록실(-OH) 모이어티를 함유하는 아미노산을 지칭한다. 유전자에 의해 코딩되는 하이드록실 함유 아미노산에는 L-Ser(S), L-Thr(T) 및 L-Tyr(Y)이 포함된다.

본원에서 사용되는 바와 같이, "폴리뉴클레오타이드" 및 "핵산"은 서로 공유 결합된 2개 이상의 뉴클레오타이드를 지칭한다. 폴리뉴클레오타이드는 전적으로 리보뉴클레오타이드(즉, RNA)로 이루어지거나, 전적으로 2' 데옥시리보뉴클레오타이드(즉, DNA)로 이루어지거나, 또는 리보- 및 2' 데옥시리보뉴클레오타이드의 혼합물로 이루어질 수 있다. 뉴클레오사이드는 전형적으로 표준 포스포디에스테르 결합을 통해 서로 연결될 것이지만, 폴리뉴클레오타이드는 하나 이상의 비표준 결합을 포함할 수 있다. 폴리뉴클레오타이드는 단일 가닥 또는 이중 가닥일 수 있거나, 또는 단일 가닥 영역 및 이중 가닥 영역 둘 모두를 포함할 수 있다. 또한, 폴리뉴클레오타이드는 전형적으로 자연 발생 코딩 핵염기(즉, 아데닌, 구아닌, 우라실, 티민 및 시토신)로 이루어질 것이지만, 그것은 하나 이상의 변형된 핵염기 및/또는 합성 핵염기, 예를 들어, 이노신, 잔틴, 히포잔틴 등을 포함할 수 있다. 일부 실시양태에서, 이러한 변형된 핵염기 또는 합성 핵염기는 아미노산 서열을 코딩하는 핵염기이다.

본원에서 사용되는 바와 같이, "뉴클레오사이드"는 핵염기(즉, 질소 염기) 및 5-탄소 당(예를 들어, 리보스 또는 데옥시리보스)을 포함하는 글리코실아민을 지칭한다. 뉴클레오사이드의 비제한적인 예는 시티딘, 우리딘, 아데노신, 구아노신, 티미딘 및 이노신을 포함한다. 이와 대조적으로, 용어 "뉴클레오타이드"는 핵염기, 5-탄소 당 및 하나 이상의 포스페이트 기를 포함하는 글리코실아민을 지칭한다. 일부 실시양태에서, 뉴클레오사이드는 뉴클레오타이드를 생성하기 위해 키나제에 의해 인산화될 수 있다.

본원에서 사용되는 바와 같이, "뉴클레오사이드 디포스페이트"는 핵염기(즉, 질소 염기), 5-탄소 당(예를 들어, 리보스 또는 데옥시리보스), 및 디포스페이트(즉, 피로포스페이트) 모이어티를 포함하는 글리코실아민을 지칭한다. 본원의 일부 실시양태에서, "뉴클레오사이드 디포스페이트"는 "NDP"로 약칭된다. 뉴클레오사이드 디포스페이트의 비제한적인 예는 시티딘 디포스페이트(CDP), 우리딘 디포스페이트(UDP), 아데노신 디포스페이트(ADP), 구아노신 디포스페이트(GDP), 티미딘 디포스페이트(TDP) 및 이노신 디포스페이트(IDP)를 포함한다. 용어 "뉴클레오사이드" 및 "뉴클레오타이드"는 일부 문맥에서 상호 교환 가능하게 사용될 수 있다.

본원에서 사용되는 바와 같이, "코딩 서열"은 단백질의 아미노산 서열을 코딩하는 핵산(예를 들어, 유전자) 부분을 지칭한다.

본원에서 사용되는 바와 같이, 용어 "생촉매반응," "생촉매성," "생체변환," 및 "생합성"은 유기 화합물에 대한 화학 반응을 수행하는 효소의 사용을 지칭한다.

본원에서 사용되는 바와 같이, "야생형" 및 "자연 발생"은 자연계에서 발견되는 형태를 지칭한다. 예를 들어, 야생형 폴리펩타이드 또는 폴리뉴클레오타이드 서열은 자연계의 공급원으로부터 단리될 수 있으며 인간 조작에 의해 의도적으로 변형되지 않은 유기체 내에 존재하는 서열이다.

본원에서 사용되는 바와 같이, 세포, 핵산, 또는 폴리펩타이드와 함께 사용될 때 "재조합", "조작된" 및 "비자연 발생"은 자연계에 존재하지 않을 방식으로 변형된 물질, 또는 상기 물질의 자연 또는 천연 형태에 상응하는 물질을 지칭한다. 일부 실시양태에서, 세포, 핵산 또는 폴리펩타이드는 자연 발생 세포, 핵산 또는 폴리펩타이드와 동일하지만, 합성 물질로부터 및/또는 재조합 기술을 사용한 조작에 의해 생산되거나 유래된다. 비제한적인 예는 특히 세포의 천연(비-재조합)) 형태 내에서 발견되지 않는 유전자를 발현하는 재조합 세포 또는 상이한 수준으로 발현되는 천연 유전자를 발현하는 재조합 세포를 포함한다.

용어 "서열 동일성 비율(%)"은 폴리뉴클레오타이드 또는 폴리펩타이드 사이의 비교를 지칭하기 위해 본원에서 사용되며, 비교 윈도우에서 최적으로 정렬된 2개의 서열을 비교함으로써 결정되고, 여기서 비교 윈도우 내의 폴리뉴클레오타이드 또는 폴리펩타이드 서열의 부분은 2개의 서열의 최적 정렬을 위해 기준 서열과 비교하여 부가 또는 결실(즉, 갭)을 포함할 수 있다. 백분율은 동일한 핵산 염기 또는 아미노산 잔기가 두 서열에 존재하는 위치의 수를 결정하여 매치된 위치의 수를 산출하는 단계, 매치된 위치의 수를 비교 윈도우 내의 위치의 총 수로 나누고 그 결과에 100을 곱하여 서열 동일성의 백분율을 산출하는 단계에 의해 계산될 수 있다. 대안적으로, 백분율은 동일한 핵산 염기 또는 아미노산 잔기가 두 서열에 존재하거나 핵산 염기 또는 아미노산 잔기가 갭과 정렬되는 위치의 수를 결정하여 매치된 위치의 수를 산출하는 단계, 매치된 위치의 수를 비교 윈도우 내의 위치의 총 수로 나누고 그 결과에 100을 곱하여 서열 동일성의 백분율을 산출하는 단계에 의해 계산될 수 있다. 관련 기술 분야에서 통상의 기술을 가진 자는 2개의 서열을 정렬하는데 이용가능한 많은 확립된 알고리즘이 있다는 것을 이해한다. 비교를 위한 서열의 최적 정렬은, 관련 기술 분야에 공지된 바와 같이, 스미스(Smith) 및 워터만(Waterman)의 국소 상동성 알고리즘(Smith and Waterman, Adv. Appl. Math., 2:482 [1981]), 니들만(Needleman) 및 분쉬(Wunsch)의 상동성 정렬 알고리즘(Needleman and Wunsch, J. Mol., Biol., 48:443 [1970]), 피어슨(Pearson) 및 립만(Lipman)의 유사성 검색 방법(Pearson and Lipman, Proc. Natl. Acad. Sci. USA 85:2444 [1988]), 이들 알고리즘의 컴퓨터에 의한 실행(예를 들어, GCG 위스콘신 소프트웨어 패키지 내의 GAP, BESTFIT, FASTA, 및 TFASTA), 또는 육안 검사를 포함하고 이로 제한되지 않는 임의의 적합한 방법에 의해 수행될 수 있다. 서열 동일성 및 서열 유사성 비율을 결정하는데 적합한 알고리즘의 예는 BLAST 및 BLAST 20 알고리즘을 포함하지만 이로 제한되지 않으며, 이는 알츌(Altschul) 등에 의해 설명되어 있다(각각, 문헌 [Altschul et al., J. Mol. Biol., 215: 403-410 [1990]; 및 [Altschul et al., Nucl. Acids Res., 3389-3402 [1977] 참조). BLAST 분석을 수행하기 위한 소프트웨어는 미국 국립 생물 정보 센터 웹사이트를 통해 공개적으로 이용가능하다. 이 알고리즘은 데이터베이스 서열 내의 동일한 길이의 워드와 정렬될 때 일부 양의 값의 역치 스코어 T와 일치하거나 이를 충족하는, 질의 서열 내의 길이 W의 짧은 워드를 확인함으로써 높은 스코어링 서열 쌍(HSP)을 먼저 확인하는 것을 포함한다. T는 이웃 워드 스코어 역치로도 불린다(상기 문헌 [Altschul et al.] 참조). 이들 초기 이웃 워드 히트(hit)는 이들을 함유하는 더 긴 HSP를 찾기 위한 검색을 개시하는 씨드(seed)로서 작용한다. 이후, 워드 히트는 누적 정렬 스코어가 증가될 수 있을 때까지 각각의 서열을 따라 양 방향으로 연장된다. 누적 스코어는 뉴클레오타이드 서열에 대해, 파라미터 M(매칭 잔기 쌍에 대한 보상 스코어; 항상 >0) 및 N(미스매칭 잔기에 대한 페널티 스코어; 항상 <0)을 사용하여 계산된다. 아미노산 서열의 경우, 누적 스코어를 계산하기 위해 스코어링 매트릭스(scoring matrix)가 사용된다. 각각의 방향에서 워드 히트의 연장은 누적 정렬 스코어가 그의 최대 달성 값으로부터 양 X만큼 떨어질 때; 누적 스코어가 하나 이상의 음성 스코어링 잔기 정렬의 누적으로 인해 0 이하가 될 때; 또는 하나의 서열의 말단에 도달할 때 중지된다. BLAST 알고리즘 파라미터 W, T, 및 X는 정렬의 민감성 및 속도를 결정한다. BLASTN 프로그램(뉴클레오타이드 서열용)은 디폴트로서 워드 길이(W) 11, 기대값(E) 10, M=5, N=-4, 및 두 가닥의 비교를 사용한다. 아미노산 서열의 경우, BLASTP 프로그램은 디폴트로서 워드 길이(W) 3, 기대값(E) 10, 및 BLOSUM62 스코어링 매트릭스를 사용한다(문헌 [Henikoff and Henikoff, Proc. Natl. Acad. Sci. USA 89:10915 [1989]] 참조). 서열 정렬 및 서열 동일성 %의 예시적인 결정은 제공된 디폴트 파라미터를 사용하여, GCG 위스콘신 소프트웨어 패키지(Accelrys, 미국 위스콘신주 매디슨 소재) 내의 BESTFIT 또는 GAP 프로그램을 사용할 수 있다.

"기준 서열"은 서열 및/또는 활성 비교를 위한 기준으로서 사용되는 정의된 서열을 지칭한다. 기준 서열은 더 큰 서열의 서브세트, 예를 들어, 전장 유전자 또는 폴리펩타이드 서열의 세그먼트일 수 있다. 일반적으로, 기준 서열은 핵산 또는 폴리펩타이드의 적어도 20개 뉴클레오타이드 또는 아미노산 잔기 길이, 적어도 25개 잔기 길이, 적어도 50개 잔기 길이, 적어도 100개 잔기 길이 또는 전장이다. 2개의 폴리뉴클레오타이드 또는 폴리펩타이드는 각각 (1) 2개의 서열 사이에 유사한 서열(즉, 완전한 서열의 일부)을 포함할 수 있고, 및 (2) 2개의 서열 사이에 상이한 서열을 추가로 포함할 수 있으므로, 2개(또는 그 초과)의 폴리뉴클레오타이드 또는 폴리펩타이드 사이의 서열 비교는 전형적으로 "비교 윈도우"에서 2개의 폴리뉴클레오타이드 또는 폴리펩타이드의 서열을 비교하여 서열 유사성의 국소 영역을 확인하고 비교함으로써 수행된다. 일부 실시양태에서, "기준 서열"은 일차 아미노산 서열에 기초할 수 있으며, 여기서 기준 서열은 일차 서열에서 하나 이상의 변화를 가질 수 있는 서열이다.

본원에서 사용되는 바와 같이, "비교 윈도우"는 적어도 약 20개의 인접한 뉴클레오타이드 위치 또는 아미노산 잔기의 개념적 세그먼트를 지칭하며, 여기서 서열은 적어도 20개의 인접한 뉴클레오타이드 또는 아미노산의 기준 서열과 비교될 수 있고, 여기서 비교 윈도우 내의 서열의 일부는 2개의 서열의 최적 정렬을 위한 기준 서열(부가 또는 결실을 포함하지 않음)과 비교하여 20% 이하의 부가 또는 결실(즉, 갭)을 포함할 수 있다. 비교 윈도우는 20개 초과의 인접한 잔기일 수 있고, 선택적으로 30, 40, 50, 100개 또는 그 초과의 윈도우를 포함한다.

본원에서 사용되는 바와 같이, 주어진 아미노산 또는 폴리뉴클레오타이드 서열의 넘버링의 측면에서 사용될 때, "에 상응하는", "에 대한" 및 "와 비교하여"는 주어진 아미노산 또는 폴리뉴클레오타이드 서열이 기준 서열과 비교될 때 특정 기준 서열의 잔기의 넘버링을 지칭한다. 즉, 주어진 중합체의 잔기 번호 또는 잔기 위치는 주어진 아미노산 또는 폴리뉴클레오타이드 서열 내의 잔기의 실제 수치적 위치에 의해서가 아니라 기준 서열에 대해 지정된다. 예를 들어, 조작된 포스포펜토뮤타제의 아미노산 서열과 같은 주어진 아미노산 서열은 2개의 서열 사이의 잔기 매치를 최적화하기 위해 갭을 도입함으로써 기준 서열에 정렬될 수 있다. 이 경우, 비록 갭이 존재하지만, 주어진 아미노산 또는 폴리뉴클레오타이드 서열에서의 잔기의 넘버링은 그것이 정렬된 기준 서열에 대해 이루어진다.

본원에서 사용되는 바와 같이, "실질적인 동일성"은 적어도 20개 잔기 위치의 비교 윈도우에서, 흔히 적어도 30-50개 잔기의 윈도우에서 기준 서열과 비교하여 적어도 80 퍼센트의 서열 동일성, 적어도 85 퍼센트의 동일성, 적어도 89 내지 95 퍼센트의 서열 동일성, 또는 보다 일반적으로, 적어도 99 퍼센트의 서열 동일성을 갖는 폴리뉴클레오타이드 또는 폴리펩타이드 서열을 지칭하며, 여기서 서열 동일성의 백분율은 비교 윈도우에서 기준 서열을 기준 서열의 총 20 퍼센트 이하의 결실 또는 부가를 포함하는 서열과 비교함으로써 계산된다. 폴리펩타이드에 적용된 일부 특정 실시양태에서, 용어 "실질적인 동일성"은 2개의 폴리펩타이드 서열이 예를 들어 디폴트 갭 가중치를 사용하여 프로그램 GAP 또는 BESTFIT에 의해 최적으로 정렬될 때, 적어도 80 퍼센트의 서열 동일성, 바람직하게는 적어도 89 퍼센트의 서열 동일성, 적어도 95 퍼센트의 서열 동일성 또는 그 초과의 서열 동일성(예를 들어, 99 퍼센트의 서열 동일성)을 공유하는 것을 의미한다. 일부 실시양태에서, 비교되는 서열에서 동일하지 않은 잔기 위치는 보존적 아미노산 치환에 의해 달라진다.

본원에서 사용되는 바와 같이, "아미노산 차이" 및 "잔기 차이"는 기준 서열 내의 상응하는 위치에 있는 아미노산 잔기와 비교하여 폴리펩타이드 서열의 위치에서의 아미노산 잔기의 차이를 지칭한다. 일부 경우에, 기준 서열에는 히스티딘 태그가 있지만, 넘버링은 히스티딘 태그가 없는 동등한 기준 서열에서와 같이 유지된다. 아미노산 차이의 위치는 일반적으로 본원에서 "Xn"으로 지칭되며, 여기서 n은 잔기 차이가 기초로 하는 기준 서열 내의 상응하는 위치를 지칭한다. 예를 들어, "서열 번호 4와 비교하여 위치 X93에서의 잔기 차이"는 서열 번호 4의 위치 93에 상응하는 폴리펩타이드 위치에서의 아미노산 잔기의 차이를 지칭한다. 따라서, 서열 번호 4의 참고 폴리펩타이드가 위치 93에 세린을 가지면, "서열 번호 4와 비교하여 위치 X93에서의 잔기 차이"는 서열 번호 4의 위치 93에 상응하는 폴리펩타이드의 위치에서의 세린 이외의 임의의 다른 잔기의 아미노산 치환을 지칭한다. 본원에서 대부분의 경우, 위치에서의 특정 아미노산 잔기 차이는 "XnY"로 표시되며, 여기서 "Xn"은 전술한 바와 같이 상응하는 위치를 특정하고, "Y"는 조작된 폴리펩타이드에서 발견되는 아미노산(즉, 참고 폴리펩타이드와 상이한 잔기)의 단일 글자 식별자이다. 일부 경우에(예를 들어, 실시예에서 제시되는 표에서), 본 발명은 또한 종래의 표기법 "AnB"에 의해 표시된 특정 아미노산 차이를 제공하며, 여기서 A는 기준 서열에서의 잔기의 단일 글자 식별자이고, "n"은 기준 서열에서의 잔기 위치의 번호이며, B는 조작된 폴리펩타이드의 서열에서의 잔기 치환의 단일 글자 식별자이다. 일부 경우에, 본 발명의 폴리펩타이드는 기준 서열과 비교하여 하나 이상의 아미노산 잔기 차이를 포함할 수 있고, 이는 기준 서열과 비교하여 잔기 차이가 존재하는 특정 위치의 목록에 의해 표시된다. 일부 실시양태에서, 둘 이상의 아미노산이 폴리펩타이드의 특정 잔기 위치에서 사용될 수 있는 경우, 사용될 수 있는 다양한 아미노산 잔기는 "/"에 의해 분리된다(예를 들어, X307H/X307P 또는 X307H/P). 슬래시는 또한 주어진 변이체 내의 다수의 치환을 나타내기 위해 사용될 수 있다(즉, 조합 변이체에서와 같이, 주어진 서열에 둘 이상의 치환이 존재함). 일부 실시양태에서, 본 발명은 보존적 또는 비보존적 아미노산 치환을 포함하는 하나 이상의 아미노산 차이를 포함하는 조작된 폴리펩타이드 서열을 포함한다. 일부 추가의 실시양태에서, 본 발명은 보존적 및 비보존적 아미노산 치환을 모두 포함하는 조작된 폴리펩타이드 서열을 제공한다.

본원에서 사용되는 바와 같이, "보존적 아미노산 치환"은 잔기를 유사한 측쇄를 갖는 상이한 잔기로 치환하는 것을 지칭하며, 따라서 전형적으로 폴리펩타이드 내의 아미노산을 동일하거나 유사한 정의된 아미노산 클래스 내의 아미노산으로 치환하는 것을 포함한다. 비제한적인 예를 들면, 일부 실시양태에서, 지방족 측쇄를 갖는 아미노산은 또 다른 지방족 아미노산(예를 들어, 알라닌, 발린, 류신, 및 이소류신)으로 치환되고/되거나; 하이드록실 측쇄를 갖는 아미노산은 하이드록실 측쇄를 갖는 또 다른 아미노산(예를 들어, 세린 및 트레오닌)으로 치환되고/되거나; 방향족 측쇄를 갖는 아미노산은 방향족 측쇄를 갖는 또 다른 아미노산(예를 들어, 페닐알라닌, 티로신, 트립토판, 및 히스티딘)으로 치환되고/되거나; 염기성 측쇄를 갖는 아미노산은 염기성 측쇄를 갖는 또 다른 아미노산(예를 들어, 리신 및 아르기닌)으로 치환되고/되거나; 산성 측쇄를 갖는 아미노산은 산성 측쇄를 갖는 또 다른 아미노산(예를 들어, 아스파르트산 또는 글루탐산)으로 치환되고/되거나; 소수성 또는 친수성 아미노산은 각각 또 다른 소수성 또는 친수성 아미노산으로 대체된다.

본원에서 사용되는 바와 같이, "비보존적 치환"은 폴리펩타이드 내의 아미노산을 유의하게 상이한 측쇄 특성을 갖는 아미노산으로 치환하는 것을 지칭한다. 비보존적 치환은 정의된 기 내에서보다는 정의된 기 사이에서 아미노산을 사용할 수 있고, (a) 치환 영역 내의 펩타이드 백본의 구조(예를 들어, 글리신을 프롤린으로), (b) 전하 또는 소수성, 또는 (c) 측쇄의 부피에 영향을 미친다. 비제한적인 예를 들어, 예시적인 비보존적 치환은 염기성 또는 지방족 아미노산으로 치환된 산성 아미노산; 작은 아미노산으로 치환된 방향족 아미노산; 및 소수성 아미노산으로 치환된 친수성 아미노산일 수 있다.

본원에서 사용되는 바와 같이, "결실"은 참고 폴리펩타이드로부터 하나 이상의 아미노산의 제거에 의한 폴리펩타이드의 변형을 지칭한다. 결실은 조작된 포스포펜토뮤타제 효소의 효소 활성을 유지하고/하거나 개선된 특성을 유지하면서 1개 이상의 아미노산, 2개 이상의 아미노산, 5개 이상의 아미노산, 10개 이상의 아미노산, 15개 이상의 아미노산, 또는 20개 이상의 아미노산, 참고 효소를 구성하는 아미노산의 총 수의 최대 10%, 또는 아미노산의 총 수의 최대 20%의 제거를 포함할 수 있다. 결실은 폴리펩타이드의 내부 부위 및/또는 말단 부위에 관한 것일 수 있다. 다양한 실시양태에서, 결실은 연속적인 세그먼트을 포함할 수 있거나 불연속적일 수 있다. 결실은 일반적으로 아미노산 서열에 "-"로 표시된다.

본원에서 사용되는 바와 같이, "삽입"은 참고 폴리펩타이드로부터 하나 이상의 아미노산의 부가에 의한 폴리펩타이드에 대한 변형을 지칭한다. 삽입은 폴리펩타이드의 내부 부분, 또는 카르복시 또는 아미노 말단에 있을 수 있다. 본원에서 사용되는 바와 같이 삽입은 관련 기술 분야에 공지된 바와 같은 융합 단백질을 포함한다. 삽입은 아미노산의 인접한 세그먼트일 수 있거나 또는 자연 발생 폴리펩타이드 내의 아미노산 중 하나 이상에 의해 분리될 수 있다.

용어 "아미노산 치환 세트" 또는 "치환 세트"는 기준 서열과 비교하여 폴리펩타이드 서열 내의 아미노산 치환의 군을 지칭한다. 치환 세트는 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15개 또는 그 초과의 아미노산 치환을 가질 수 있다. 일부 실시양태에서, 치환 세트는 실시예에서 제공되는 표에 나열된 임의의 변이체 포스포펜토뮤타제에 존재하는 아미노산 치환 세트를 지칭한다.

"기능적 단편" 및 "생물학적 활성 단편"은 아미노 말단 및/또는 카르복시 말단 결실(들) 및/또는 내부 결실을 갖지만, 나머지 아미노산 서열은 비교되는 서열(예를 들어, 본 발명의 조작된 전장 포스포펜토뮤타제) 내의 상응하는 위치와 동일하고 전장 폴리펩타이드의 활성을 실질적으로 모두 보유하는 폴리펩타이드를 지칭하기 위해 본원에서 상호 교환 가능하게 사용된다.

본원에서 사용되는 바와 같이, "단리된 폴리펩타이드"는 자연적으로 동반되는 다른 오염물질(예를 들어, 단백질, 지질, 및 폴리뉴클레오타이드)로부터 실질적으로 분리된 폴리펩타이드를 지칭한다. 이 용어는 이들의 자연 발생 환경 또는 발현 시스템(예를 들어, 숙주 세포 내에서 또는 시험관내 합성을 통해)으로부터 제거되거나 정제된 폴리펩타이드를 포함한다. 재조합 포스포펜토뮤타제 폴리펩타이드는 세포 내에 존재하거나, 세포 배지에 존재하거나, 또는 용해물 또는 단리된 제제와 같은 다양한 형태로 제조될 수 있다. 따라서, 일부 실시양태에서, 재조합 포스포펜토뮤타제 폴리펩타이드는 단리된 폴리펩타이드일 수 있다.

본원에서 사용되는 바와 같이, "실질적으로 순수한 폴리펩타이드"는 폴리펩타이드 종이 존재하는 우세한 종(즉, 몰 또는 중량 기준으로, 조성물 내의 임의의 다른 개별적인 거대분자 종보다 더 풍부함)인 조성물을 지칭하며, 일반적으로 대상 종이 존재하는 거대분자 종의 몰 또는 중량% 기준으로 적어도 약 50 퍼센트를 포함할 때 실질적으로 정제된 조성물이다. 그러나, 일부 실시양태에서, 포스포펜토뮤타제를 포함하는 조성물은 50% 미만으로 순수한(예를 들어, 약 10%, 약 20%, 약 30%, 약 40%, 또는 약 50%) 포스포펜토뮤타제를 포함한다. 일반적으로, 실질적으로 순수한 포스포펜토뮤타제 조성물은 조성물에 존재하는 모든 거대분자 종의 몰 또는 중량% 기준으로 약 60% 이상, 약 70% 이상, 약 80% 이상, 약 90% 이상, 약 95% 이상, 및 약 98% 또는 그 초과를 포함한다. 일부 실시양태에서, 대상 종은 본질적인 균질성(즉, 오염 종은 종래의 검출 방법에 의해 조성물에서 검출될 수 없음)까지 정제되며, 여기서 조성물은 본질적으로 단일 거대분자 종으로 이루어진다. 용매 종, 작은 분자(<500 달톤), 및 원소 이온 종은 거대분자 종으로 간주되지 않는다. 일부 실시양태에서, 단리된 재조합 포스포펜토뮤타제 폴리펩타이드는 실질적으로 순수한 폴리펩타이드 조성물이다.

본원에서 사용되는 바와 같이, "개선된 효소 특성"은 효소의 적어도 하나의 개선된 특성을 지칭한다. 일부 실시양태에서, 본 발명은 기준 포스포펜토뮤타제 폴리펩타이드 및/또는 야생형 포스포펜토뮤타제 폴리펩타이드, 및/또는 또 다른 조작된 포스포펜토뮤타제 폴리펩타이드와 비교하여 임의의 효소 특성의 개선을 나타내는 조작된 포스포펜토뮤타제 폴리펩타이드를 제공한다. 따라서, "개선"의 수준은 야생형뿐만 아니라 조작된 포스포펜토뮤타제를 포함하는, 다양한 포스포펜토뮤타제 폴리펩타이드 사이에서 결정되고 비교될 수 있다. 개선된 특성은 단백질 발현 증가, 열활성 증가, 열안정성 증가, pH 활성 증가, 안정성 증가, 효소 활성 증가, 기질 특이성 또는 친화성 증가, 특이적 활성 증가, 기질 또는 최종 생성물 억제에 대한 내성 증가, 화학적 안정성 증가, 화학적 선택성 개선, 용매 안정성 개선, 산성 pH에 대한 내성 증가, 단백질 분해 활성에 대한 내성 증가(즉, 단백질 분해에 대한 민감성 감소), 응집 감소, 용해도 증가, 및 온도 프로파일 변경과 같은 특성을 포함하지만, 이로 제한되지 않는다. 추가의 실시양태에서, 상기 용어는 포스포펜토뮤타제 효소의 적어도 하나의 개선된 특성과 관련하여 사용된다. 일부 실시양태에서, 본 발명은 기준 포스포펜토뮤타제 폴리펩타이드 및/또는 야생형 포스포펜토뮤타제 폴리펩타이드 및/또는 다른 조작된 포스포펜토뮤타제 폴리펩타이드와 비교하여 임의의 효소 특성에서 개선을 나타내는 조작된 포스포펜토뮤타제 폴리펩타이드를 제공한다. 따라서, "개선" 수준은 야생형을 포함한 다양한 포스포펜토뮤타제 폴리펩타이드 및 조작된 포스포펜토뮤타제 사이에서 결정되고 비교할 수 있다.

본원에서 사용되는 바와 같이, "증가된 효소적 활성" 및 "향상된 촉매 활성"은 조작된 폴리펩타이드의 개선된 특성을 지칭하며, 이는 기준 효소와 비교하여 특이적 활성(예를 들어, 생성된 생성물/시간/중량 단백질)의 증가 또는 기질로부터 생성물로의 전환 비율(예를 들어, 효소의 특정 양을 사용하여 특정 시간 내에 기질의 출발 양에서 생성물로의 전환 비율)의 증가에 의해 표시될 수 있다. 일부 실시양태에서, 상기 용어는 본원에서 제공되는 조작된 포스포펜토뮤타제 폴리펩타이드의 개선된 특성을 지칭하며, 이는 기준 포스포펜토뮤타제 효소와 비교하여 특이적 활성(예를 들어, 생성된 생성물/시간/중량 단백질)의 증가 또는 기질로부터 생성물로의 전환 비율(예를 들어, 포스포펜토뮤타제의 특정 양을 사용하여 특정 시간 내에 기질의 출발 양에서 생성물로의 전환 비율)의 증가에 의해 표시될 수 있다. 일부 실시양태에서, 상기 용어는 본원에서 제공되는 개선된 포스포펜토뮤타제 효소와 관련하여 사용된다. 본 발명의 조작된 포스포펜토뮤타제의 효소 활성을 결정하는 예시적인 방법이 실시예에 제공되어 있다. K _m , V _max 또는 k _cat 의 고전적인 효소 특성을 포함하는 효소 활성과 관련된 임의의 특성은 영향을 받을 수 있고, 이들의 변화는 효소적 활성을 증가시킬 수 있다. 예를 들어, 효소 활성의 개선은 상응하는 야생형 효소의 효소 활성의 약 1.1배로부터, 자연 발생 포스포펜토뮤타제 또는 포스포펜토뮤타제 폴리펩타이드가 그로부터 유래되는 또 다른 조작된 포스포펜토뮤타제보다 2배, 5배, 10배, 20배, 25배, 50배, 75배, 100배, 150배, 200배 또는 그 초과의 효소 활성만큼 클 수 있다.

본원에서 사용되는 바와 같이, "전환"은 기질(들)에서 상응하는 생성물(들)로의 효소에 의한 전환(또는 생체변환)을 지칭한다. "전환 비율"은 특정 조건 하에 일정 기간 내에 생성물로 전환되는 기질의 비율을 지칭한다. 따라서, 포스포펜토뮤타제 폴리펩타이드의 "효소 활성" 또는 "활성"은 특정 시간 내에 기질의 생성물로의 "전환 비율"로 표현될 수 있다.

"전반적인(generalist) 특성"을 갖는 효소(또는 "전반적인(generalist) 효소")는 모 서열과 비교하여 광범위한 기질에 대해 개선된 활성을 나타내는 효소를 지칭한다. 전반적인 효소는 반드시 모든 가능한 기질에 대해 개선된 활성을 나타내는 것은 아니다. 일부 실시양태에서, 본 발명은 광범위한 입체적으로 및 전자적으로 다양한 기질에 대해서 모 유전자와 비교하여 유사하거나 개선된 활성을 나타낸다는 점에서 전반적인 특성을 갖는 포스포펜토뮤타제 변이체를 제공한다. 또한, 본원에서 제공되는 전반적인 효소는 대사산물/생성물의 생산을 증가시키기 위해 광범위한 다양한 분자에 대해 개선되도록 조작되었다.

용어 "엄격한 혼성화 조건"은 핵산 혼성체가 안정한 조건을 지칭하기 위해 본원에서 사용된다. 관련 기술 분야에서 통상의 기술을 가진 자에게 공지된 바와 같이, 혼성체의 안정성은 혼성체의 융점(T _m )에 반영된다. 일반적으로, 혼성체의 안정성은 이온 강도, 온도, G/C 함량, 및 카오트로픽제(chaotropic agent)의 존재의 함수이다. 폴리뉴클레오타이드의 T _m 값은 융점을 예측하는 공지된 방법을 사용하여 계산될 수 있다(예를 들어, 문헌 [Baldino et al., Meth. Enzymol., 168:761-777 [1989]; Bolton et al., Proc. Natl. Acad. Sci. USA 48:1390 [1962]; Bresslauer et al., Proc. Natl. Acad. Sci. USA 83:8893-8897 [1986]; Freier et al., Proc. Natl. Acad. Sci. USA 83:9373-9377 [1986]; Kierzek et al., Biochem., 25:7840-7846 [1986]; Rychlik et al., Nucl. Acids Res., 18:6409-6412 [1990](erratum, Nucl. Acids Res., 19:698 [1991]); Sambrook et al., supra); Suggs et al., 1981, in Developmental Biology Using Purified Genes, Brown et al. [eds.], pp. 683-693, Academic Press, Cambridge, MA [1981]; 및 Wetmur, Crit. Rev. Biochem. Mol. Biol. 26:227-259 [1991] 참조). 일부 실시양태에서, 폴리뉴클레오타이드는 본원에서 개시되는 폴리펩타이드를 코딩하며, 중간 정도로 엄격한 또는 매우 엄격한 조건과 같은 정의된 조건 하에 본 발명의 조작된 포스포펜토뮤타제 효소를 코딩하는 서열의 보체에 혼성화한다.

본원에서 사용되는 바와 같이, "혼성화 엄격성"은 핵산의 혼성화에서 세척 조건과 같은 혼성화 조건에 관한 것이다. 일반적으로, 혼성화 반응은 더 낮은 엄격성의 조건 하에서 수행된 다음, 다양하지만 더 높은 엄격성의 세척이 수행된다. 용어 "중간 정도의 엄격한 혼성화"는 표적-DNA가 표적 DNA에 대해 약 60%의 동일성, 바람직하게는 약 75%의 동일성, 약 85%의 동일성, 표적-폴리뉴클레오타이드에 대해 약 90% 초과의 동일성을 갖는 상보적인 핵산에 결합하게 하는 조건을 지칭한다. 예시적인 중간 정도의 엄격한 조건은 42℃에서 50% 포름아미드, 5x 덴하르트 용액, 5x SSPE, 0.2% SDS에서의 혼성화 후, 42℃에서 0.2xSSPE, 0.2% SDS에서의 세척과 동등한 조건이다. "높은 엄격성 혼성화"는 일반적으로 정의된 폴리뉴클레오타이드 서열에 대해 용액 조건 하에서 결정된 바와 같은 열 융점 T _m 으로부터 약 10℃ 이하인 조건을 지칭한다. 일부 실시양태에서, 높은 엄격성 조건은 65℃에서 0.018 M NaCl에서 안정한 혼성체를 형성하는 핵산 서열만의 혼성화를 허용하는 조건을 지칭한다(즉, 혼성체가 65℃에서 0.018 M NaCl에서 안정하지 않으면, 이것은 본원에서 고려되는 바와 같은 높은 엄격성 조건 하에서 안정하지 않을 것임). 높은 엄격성 조건은 예를 들어 42℃에서 50% 포름아미드, 5x 덴하르트 용액, 5x SSPE, 0.2% SDS와 동등한 조건에서의 혼성화 후, 65℃에서 0.1x SSPE, 및 0.1% SDS에서의 세척에 의해 제공될 수 있다. 또 다른 높은 엄격성 조건은 65℃에서 0.1%(w/v) SDS를 함유하는 5x SSC에서의 혼성화와 동등한 조건에서의 혼성화 및 65℃에서 0.1% SDS를 함유하는 0.1x SSC에서의 세척이다. 다른 높은 엄격성 혼성화 조건뿐만 아니라 중간 정도의 엄격한 조건은 상기 인용된 참고문헌에 기재되어 있다.

본원에서 사용되는 바와 같이, "코돈 최적화된"은 단백질을 코딩하는 폴리뉴클레오타이드의 코돈이 코딩된 단백질이 관심있는 유기체에서 효율적으로 발현되도록 특정 유기체에서 우선적으로 사용되는 것으로 변화하는 것을 지칭한다. 유전자 코드는 대부분의 아미노산이 "동의어" 또는 "동의" 코돈으로 불리는 몇 개의 코돈에 의해 표시된다는 점에서 축퇴성이지만, 특정 유기체에 의한 코돈 사용 빈도는 무작위적이 아니며, 특정 코돈 삼중자(triplet)로 편향된다는 것이 널리 알려져 있다. 이 코돈 사용 빈도 편향은 주어진 유전자, 공통 기능 또는 조상 기원의 유전자, 고도로 발현된 단백질 대 낮은 카피수 단백질, 및 유기체 게놈의 전체적인 단백질 코딩 영역과 관련하여 더 높을 수 있다. 일부 실시양태에서, 포스포펜토뮤타제 효소를 코딩하는 폴리뉴클레오타이드는 발현을 위해 선택된 숙주 유기체에서 최적 생산을 위해 코돈 최적화될 수 있다.

본원에서 사용되는 바와 같이, "바람직한", "최적" 및 "높은 코돈 사용 빈도 편향" 코돈은 단독으로 또는 조합하여 사용될 때 동일한 아미노산을 코딩하는 다른 코돈보다 단백질 코딩 영역에서 더 높은 빈도로 사용되는 코돈을 상호 교환 가능하게 지칭한다. 바람직한 코돈은 단일 유전자, 공통 기능 또는 기원의 유전자 세트, 고도로 발현된 유전자에서의 코돈 사용 빈도, 전체 유기체의 전체적인 단백질 코딩 영역에서의 코돈 빈도, 관련 유기체의 전체적인 단백질 코딩 영역에서의 코돈 빈도, 또는 이들의 조합과 관련하여 결정될 수 있다. 유전자 발현 수준에 따라 그 빈도가 증가하는 코돈은 전형적으로 발현을 위한 최적 코돈이다. 특정 유기체에서 코돈 빈도(예를 들어, 코돈 사용 빈도, 상대적인 동의 코돈 사용 빈도) 및 코돈 선호도를 결정하기 위한 다양한 방법이 알려져 있으며, 이는 예를 들어 클러스터 분석 또는 대응 분석(correspondence analysis), 및 유전자에 사용된 코돈의 유효 수를 사용하는 다변량 분석을 포함한다(예를 들어, GCG CodonPreference, Genetics Computer Group Wisconsin Package; CodonW, Peden, University of Nottingham; McInerney, Bioinform., 14:372-73 [1998]; Stenico et al., Nucl. Acids Res., 222437-46 [1994]; 및 Wright, Gene 87:23-29 [1990] 참조). 많은 상이한 유기체에 대해 코돈 사용 빈도 표가 이용 가능하다(예를 들어, Wada et al., Nucl. Acids Res., 20:2111-2118 [1992]; Nakamura et al., Nucl. Acids Res., 28:292 [2000]; Duret, et al., supra; Henaut and Danchin, in Escherichia coli and Salmonella , Neidhardt, et al. (eds.), ASM Press, Washington D.C., p. 2047-2066 [1996] 참조). 코돈 사용 빈도를 얻기 위한 데이터 소스는 단백질을 코딩할 수 있는 임의의 이용 가능한 뉴클레오타이드 서열에 의존할 수 있다. 이들 데이터 세트는 발현된 단백질(예를 들어, 완전한 단백질 코딩 서열-CDS), 발현된 서열 태그(ESTS), 또는 게놈 서열의 예측된 코딩 영역을 코딩하는 것으로 실제 알려진 핵산 서열을 포함한다(예를 들어, Mount, Bioinformatics: Sequence and Genome Analysis, Chapter 8, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. [ 2001]; Uberbacher, Meth. Enzymol., 266:259-281 [1996]; 및 Tiwari et al., Comput. Appl. Biosci., 13:263-270 [1997] 참조).

본원에서 사용되는 바와 같이, "제어 서열"은 본 발명의 폴리뉴클레오타이드 및/또는 폴리펩타이드의 발현에 필요하거나 유리한 모든 성분을 포함한다. 각각의 제어 서열은 폴리펩타이드를 코딩하는 핵산 서열에 대해 천연 서열이거나 외래 서열일 수 있다. 이러한 제어 서열은 리더, 폴리아데닐화 서열, 프로펩타이드 서열, 프로모터 서열, 신호 펩타이드 서열, 개시 서열 및 전사 종결자를 포함하지만, 이로 제한되지 않는다. 최소한, 제어 서열은 프로모터, 및 전사 및 번역 정지 신호를 포함한다. 제어 서열은 폴리펩타이드를 코딩하는 핵산 서열의 코딩 영역과 제어 서열의 라이게이션을 용이하게 하는 특정 제한 부위를 도입하기 위한 목적으로 링커와 함께 제공될 수 있다.

"작동가능하게 연결된"은 제어 서열이 관심있는 폴리뉴클레오타이드 및/또는 폴리펩타이드의 발현을 유도하거나 조절하도록 제어 서열이 관심있는 폴리뉴클레오타이드와 관련된 위치에 적절하게 위치되는(즉, 기능적 관계로) 배열로서 본원에서 정의된다.

"프로모터 서열"은 코딩 서열과 같은 관심있는 폴리뉴클레오타이드의 발현을 위해 숙주 세포에 의해 인식되는 핵산 서열을 지칭한다. 프로모터 서열은 관심있는 폴리뉴클레오타이드의 발현을 매개하는 전사 제어 서열을 함유한다. 프로모터는 돌연변이체 프로모터, 말단 절단된 프로모터, 및 혼성체 프로모터를 포함하는, 선택된 숙주 세포에서 전사 활성을 나타내는 임의의 핵산 서열일 수 있고, 이는 숙주 세포에 대해 동종 또는 이종인 세포외 또는 세포내 폴리펩타이드를 코딩하는 유전자로부터 수득될 수 있다.

문구 "적합한 반응 조건"은 본 발명의 포스포펜토뮤타제 폴리펩타이드가 기질을 원하는 생성물 화합물로 전환할 수 있는 효소 전환 반응 용액의 조건(예를 들어, 효소 로딩, 기질 로딩, 온도, pH, 완충제, 공용매 등의 범위)을 지칭한다. 일부 예시적인 "적합한 반응 조건"이 본원에서 제공된다.

본원에서 사용되는 바와 같이, "화합물 로딩" 또는 "효소 로딩"에서와 같은 "로딩"은 반응 시작시 반응 혼합물 내의 성분의 농도 또는 양을 지칭한다.

본원에서 사용되는 바와 같이, "효소에 의한 전환 반응 과정의 맥락에서 "기질"은 본원에서 제공되는 조작된 효소가 그에 대해 작용하는 화합물 또는 분자를 지칭한다.

본원에서 사용되는 바와 같이, 반응으로부터 생성물(예를 들어, 데옥시리보스 포스페이트 유사체)의 수율을 "증가시키는 것"은 동일한 기질 및 다른 치환체를 갖지만 관심있는 성분이 존재하지 않는 동일한 조건 하에서 수행된 반응과 비교하여, 반응 동안 존재하는 특정 성분(예를 들어, 포스포펜토뮤타제 효소)이 더 많은 생성물이 생산되게 할 때 발생한다.

특정 효소의 양이 반응을 촉매하는데 참여하는 다른 효소와 비교하여 약 2%, 약 1%, 또는 약 0.1%(wt/wt) 미만이면, 반응은 상기 특정 효소가 "실질적으로 없다"고 언급된다.

본원에서 사용되는 바와 같이, 액체(예를 들어, 배양 브로쓰)를 "분획화하는 것"은 분리 과정(예를 들어, 염 침전, 컬럼 크로마토그래피, 크기 배제, 및 여과) 또는 이러한 과정의 조합을 적용하여 원하는 단백질이 초기 액체 생성물보다 용액에서 더 큰 백분율의 총 단백질을 포함하는 용액을 제공하는 것을 의미한다.

본원에서 사용되는 바와 같이, "출발 조성물"은 적어도 하나의 기질을 포함하는 임의의 조성물을 지칭한다. 일부 실시양태에서, 출발 조성물은 임의의 기질을 포함한다.

본원에서 사용되는 바와 같이, 효소 전환 과정과 관련하여 "생성물"은 기질에 대한 효소 폴리펩타이드의 작용으로부터 생성되는 화합물 또는 분자를 의미한다.

본원에서 사용되는 바와 같이, "평형화"는 화학 반응 또는 효소에 의한 반응(예를 들어, 두 종 A 및 B의 상호전환)에서 화학물질 종의 정상 상태 농도를 초래하는 과정을 의미하며, 여기에는 화학 반응 또는 효소에 의한 반응의 순방향 속도 상수 및 역방향 속도 상수에 의해 결정되는 바와 같은 입체 이성질체의 상호전환이 포함된다.

본원에서 사용되는 바와 같이, "알킬"은 직쇄 또는 분지쇄의 1 내지 18개의 탄소 원자, 보다 바람직하게는 1 내지 8개의 탄소 원자, 가장 바람직하게는 1 내지 6개의 탄소 원자의 포화 탄화수소기를 지칭한다. 특정 수의 탄소 원자를 갖는 알킬은 괄호 안에 표시된다(예를 들어, (C1-C4)알킬은 1 내지 4개의 탄소 원자의 알킬을 의미함).

본원에서 사용되는 바와 같이, "알케닐"은 적어도 하나의 이중 결합을 함유하지만 선택적으로 하나 초과의 이중 결합을 함유하는 직쇄 또는 분지쇄의 2 내지 12개의 탄소 원자의 기를 지칭한다.

본원에서 사용되는 바와 같이, "알키닐"은 적어도 하나의 삼중 결합을 함유하지만 선택적으로 하나 초과의 삼중 결합을 함유하고 추가로 선택적으로 하나 이상의 이중 결합 모이어티를 함유하는 직쇄 또는 분지쇄의 2 내지 12개의 탄소 원자의 기를 지칭한다.

본원에서 사용되는 바와 같이, "헤테로알킬", "헤테로알케닐" 및 "헤테로알키닐"은 하나 이상의 탄소 원자가 동일하거나 상이한 헤테로원자 또는 헤테로원자성 기로 각각 독립적으로 대체된, 본원에서 정의된 바와 같은 알킬, 알케닐 및 알키닐을 지칭한다. 탄소 원자를 대체할 수 있는 헤테로원자 및/또는 헤테로원자성 기는 -O-, -S-, -S-O-, -NRα-, -PH-, -S(O)-, -S(O)₂-, -S(O)NRα-, -S(O)₂NRα- 등, 및 이들의 조합을 포함하지만 이로 제한되지 않고, 여기서 각각의 Rα는 수소, 알킬, 헤테로알킬, 시클로알킬, 헤테로시클로알킬, 아릴, 및 헤테로아릴로부터 독립적으로 선택된다.

본원에서 사용되는 바와 같이, "알콕시"는 기 -ORβ을 지칭하며, 여기서 Rβ는 본원에서 또한 정의된 바와 같은 선택적으로 치환된 알킬기를 포함하는 상기 정의된 바와 같은 알킬기이다.

본원에서 사용되는 바와 같이, "아릴"은 단일 고리(예를 들어, 페닐) 또는 다중 축합 고리(예를 들어, 나프틸 또는 안트릴)를 갖는 6 내지 12개의 탄소 원자의 불포화 방향족 카르보시클릭기를 지칭한다. 예시적인 아릴은 페닐, 피리딜, 나프틸 등을 포함한다.

본원에서 사용되는 바와 같이, "아미노"는 기 -NH₂를 지칭한다. 치환된 아미노는 기 -NHRδ, NRδRδ 및 NRδRδRδ를 지칭하며, 여기서 각각의 Rδ는 치환 또는 비치환된 알킬, 시클로알킬, 시클로헤테로알킬, 알콕시, 아릴, 헤테로아릴, 헤테로아릴알킬, 아실, 알콕시카르보닐, 술파닐, 술피닐, 설포닐 등으로부터 독립적으로 선택된다. 전형적인 아미노기는 디메틸아미노, 디에틸아미노, 트리메틸암모늄, 트리에틸암모늄, 메틸설포닐아미노, 푸라닐-옥시-설파미노 등을 포함하지만, 이로 제한되지 않는다.

본원에서 사용되는 바와 같이, "옥소"는 =0을 지칭한다.

본원에서 사용되는 바와 같이, "옥시"는 에테르 및 에스테르를 포함하는 상이한 옥시기를 형성하기 위해 다양한 치환체를 가질 수 있는 2가 기 -O-를 지칭한다.

본원에서 사용되는 바와 같이, "카르복시"는 -COOH를 의미한다.

본원에서 사용되는 바와 같이, "카르보닐"은 산, 산 할라이드, 알데하이드, 아미드, 에스테르 및 케톤을 포함하는 상이한 카르보닐기를 형성하기 위해 다양한 치환체를 가질 수 있는 -C(O)-를 지칭한다.

본원에서 사용되는 바와 같이, "알킬옥시카르보닐"은 -C(O)ORε를 지칭하고, 여기서 Rε는 선택적으로 치환될 수 있는 본원에서 정의된 바와 같은 알킬기이다.

본원에서 사용되는 바와 같이, "아미노카르보닐"은 -C(0)NH₂를 지칭한다. 치환된 아미노카르보닐은 -C(O)NRδRδ를 지칭하며, 여기서 아미노기 NRδRδ는 본원에서 정의된 바와 같다.

본원에서 사용되는 바와 같이, "할로겐" 및 "할로"는 플루오로, 클로로, 브로 모 및 요오도를 지칭한다.

본원에서 사용되는 바와 같이, "하이드록시"는 -OH를 지칭한다.

본원에서 사용되는 바와 같이, "시아노"는 -CN을 지칭한다.

본원에서 사용되는 바와 같이, "헤테로아릴"은 1 내지 10개의 탄소 원자 및 고리 내의 산소, 질소 및 황으로부터 선택되는 1 내지 4개의 헤테로원자의 방향족 헤테로사이클릭 기를 지칭한다. 이러한 헤테로아릴 기는 단일 고리(예를 들어, 피리딜 또는 푸릴) 또는 다중 축합 고리(예를 들어, 인돌리지닐 또는 벤조티에닐)를 가질 수 있다.

본원에서 사용되는 바와 같이, "헤테로아릴알킬"은 바람직하게는 알킬 모이어티에 1 내지 6개의 탄소 원자 및 헤테로아릴 모이어티에 5 내지 12개의 고리 원자를 포함하는, 헤테로아릴로 치환된 알킬(즉, 헤테로아릴-알킬 기)을 지칭한다. 이러한 헤테로아릴알킬 기는 피리딜메틸 등으로 예시된다.

본원에서 사용되는 바와 같이, "헤테로아릴알케닐"은 바람직하게는 알케닐 모이어티에 2 내지 6개의 탄소 원자 및 헤테로아릴 모이어티에 5 내지 12개의 고리 원자를 포함하는, 헤테로아릴로 치환된 알케닐(즉, 헤테로아릴-알케닐 기)을 지칭한다.

본원에서 사용되는 바와 같이, "헤테로아릴알키닐"은 바람직하게는 알키닐 모이어티에 2 내지 6개의 탄소 원자 및 헤테로아릴 모이어티에 5 내지 12개의 고리 원자를 포함하는, 헤테로아릴로 치환된 알키닐(즉, 헤테로아릴-알키닐 기)을 지칭한다.

본원에서 사용되는 바와 같이, "헤테로사이클", "헤테로사이클릭" 및 상호 교환 가능하게 "헤테로사이클로알킬"은 단일 고리 또는 다중 축합 고리, 2 내지 10개의 탄소 고리 원자 및 고리 내의 질소, 황 또는 산소로부터 선택되는 1 내지 4개의 헤테로 고리 원자를 포함하는 포화 또는 불포화 기를 지칭한다. 이러한 헤테로사이클릭 기는 단일 고리(예를 들어, 피페리디닐 또는 테트라하이드로푸릴) 또는 다중 축합 고리(예를 들어, 인돌리닐, 디하이드로벤조푸란 또는 퀴누클리디닐)를 가질 수 있다. 헤테로사이클의 예는 푸란, 티오펜, 티아졸, 옥사졸, 피롤, 이미다졸, 피라졸, 피리딘, 피라진, 피리미딘, 피리다진, 인돌리진, 이소인돌, 인돌, 인다졸, 퓨린, 퀴놀리진, 이소퀴놀린, 퀴놀린, 프탈라진, 나프틸피리딘, 퀴녹살린, 퀴나졸린, 신놀린, 프테리딘, 카르바졸, 카르볼린, 페난트리딘, 아크리딘, 페난트롤린, 이소티아졸, 페나진, 이속사졸, 페녹사진, 페노티아진, 이미다졸리딘, 이미다졸린, 피페리딘, 피페라진, 피롤리딘, 인돌린을 포함하지만, 이로 제한되지 않는다.

본원에서 사용되는 바와 같이, "원 고리"는 임의의 시클릭 구조를 포함하는 것을 의미한다. 용어 "원" 앞의 숫자는 고리를 구성하는 백본 원자의 수를 나타낸다. 따라서, 예를 들어 시클로헥실, 피리딘, 피란 및 티오피란은 6원 고리이고, 시클로펜틸, 피롤, 푸란 및 티오펜은 5원 고리이다.

달리 명시되지 않는 한, 상기한 기에서 수소가 차지하는 위치는 하이드록시, 옥소, 니트로, 메톡시, 에톡시, 알콕시, 치환된 알콕시, 트리플루오로메톡시, 할로알콕시, 플루오로, 클로로, 브로모, 요오도, 할로, 메틸, 에틸, 프로필, 부틸, 알킬, 알케닐, 알키닐, 치환된 알킬, 트리플루오로메틸, 할로알킬, 하이드록시알킬, 알콕시알킬, 티오, 알킬티오, 아실, 카르복시, 알콕시카르보닐, 카르복사미도, 치환된 카르복사미도, 알킬설포닐, 알킬설피닐, 알킬설포닐아미노, 설폰아미도, 치환된 설폰아미도, 시아노, 아미노, 치환된 아미노, 알킬아미노, 디알킬아미노, 아미노알킬, 아실아미노, 아미디노, 아미독시모, 하이드록사모일, 페닐, 아릴, 치환된 아릴, 아릴옥시, 아릴알킬, 아릴알케닐, 아릴알키닐, 피리딜, 이미다졸릴, 헤테로아릴, 치환된 헤테로아릴, 헤테로아릴옥시, 헤테로아릴알킬, 헤테로아릴알케닐, 헤테로아릴알키닐, 사이클로프로필, 사이클로부틸, 사이클로펜틸, 사이클로헥실, 사이클로알킬, 시클로알케닐, 사이클로알킬알킬, 치환된 사이클로알킬, 사이클로알킬옥시, 피롤리디닐, 피페리디닐, 모르폴리노, 헤테로사이클, (헤테로사이클)옥시, 및 (헤테로사이클)알킬을 포함하고 이로 제한되지 않는 치환체로 추가로 치환될 수 있고; 바람직한 헤테로원자는 산소, 질소 및 황이다. 개방 원자가(open valence)가 이들 치환체에 존재하는 경우, 알킬, 사이클로알킬, 아릴, 헤테로아릴 및/또는 헤테로사이클 기로 추가로 치환될 수 있고, 이러한 개방 원자가가 탄소에 존재하는 경우 할로겐 및 산소-, 질소- 또는 황-결합 치환체에 의해 추가로 치환될 수 있음이 이해되고, 그러한 개방 원자가가 여러 개 존재하는 경우, 이들 기는 결합의 직접 형성 또는 새로운 헤테로 원자, 바람직하게는 산소, 질소 또는 황에 대한 결합의 형성에 의해 결합되어 고리를 형성할 수 있다. 수소를 치환체로 대체하는 것이 본 발명의 분자에 허용되지 않는 불안정성을 도입하지 않고, 화학적으로 합리적이라면 상기 치환이 이루어질 수 있음이 추가로 이해된다.

본원에서 사용되는 바와 같이, 용어 "배양"은 임의의 적합한 조건(예를 들어, 액체, 겔 또는 고체 배지의 사용) 하에서 미생물 세포 집단의 성장을 지칭한다.

재조합 폴리펩타이드는 관련 기술 분야에 공지된 임의의 적합한 방법을 사용하여 생산될 수 있다. 관심있는 야생형 폴리펩타이드를 코딩하는 유전자는 플라스미드와 같은 벡터에서 클로닝될 수 있고, 이. 콜라이 등과 같은 원하는 숙주에서 발현될 수 있다. 재조합 폴리펩타이드의 변이체는 관련 기술 분야에 공지된 다양한 방법에 의해 생성될 수 있다. 실제로, 관련 기술 분야에서 통상의 기술을 가진 자에게 잘 알려진 매우 다양한 상이한 돌연변이 유발 기술이 존재한다. 또한, 돌연변이 유발 키트는 많은 상업용 분자 생물학 공급업체에서 입수할 수 있다. 정의된 아미노산에서 특이적 치환(부위 지정), 유전자의 국소 영역에서 특이적 또는 무작위 돌연변이(영역 특이적) 또는 전체 유전자에 대한 무작위 돌연변이(예를 들어, 포화 돌연변이 유발)를 만드는 방법이 있다. PCR, 카세트 돌연변이 유발, 유전자 합성, 오류 빈발 PCR, 셔플링을 사용한 단일 가닥 DNA 또는 이중 가닥 DNA의 부위 지정 돌연변이 유발, 및 화학적 포화 돌연변이 유발, 또는 관련 기술 분야에 공지된 임의의 다른 적합한 방법을 포함하지만 이로 제한되지 않는, 효소 변이체를 생성하기 위한 많은 적합한 방법이 관련 기술 분야에서 통상의 기술을 가진 자에게 공지되어 있다. 돌연변이 유발 및 유도 진화 방법은 발현, 스크리닝 및 분석할 수 있는 변이체 라이브러리를 생성하기 위해 효소 코딩 폴리뉴클레오타이드에 쉽게 적용될 수 있다. 임의의 적합한 돌연변이 유발 및 유도 진화 방법은 본 발명에서 유용하며, 관련 기술 분야에 잘 알려져 있다(예를 들어, 미국 특허 5,605,793, 5,811,238, 5,830,721, 5,834,252, 5,837,458, 5,928,905, 6,096,548, 6,117,679, 6,132,970, 6,165,793, 6,180,406, 6,251,674, 6,265,201, 6,277,638, 6,287,861, 6,287,862, 6,291,242, 6,297,053, 6,303,344, 6,309,883, 6,319,713, 6,319,714, 6,323,030, 6,326,204, 6,335,160, 6,335,198, 6,344,356, 6,352,859, 6,355,484, 6,358,740, 6,358,742, 6,365,377, 6,365,408, 6,368,861, 6,372,497, 6,337,186, 6,376,246, 6,379,964, 6,387,702, 6,391,552, 6,391,640, 6,395,547, 6,406,855, 6,406,910, 6,413,745, 6,413,774, 6,420,175, 6,423,542, 6,426,224, 6,436,675, 6,444,468, 6,455,253, 6,479,652, 6,482,647, 6,483,011, 6,484,105, 6,489,146, 6,500,617, 6,500,639, 6,506,602, 6,506,603, 6,518,065, 6,519,065, 6,521,453, 6,528,311, 6,537,746, 6,573,098, 6,576,467, 6,579,678, 6,586,182, 6,602,986, 6,605,430, 6,613,514, 6,653,072, 6,686,515, 6,703,240, 6,716,631, 6,825,001, 6,902,922, 6,917,882, 6,946,296, 6,961,664, 6,995,017, 7,024,312, 7,058,515, 7,105,297, 7,148,054, 7,220,566, 7,288,375, 7,384,387, 7,421,347, 7,430,477, 7,462,469, 7,534,564, 7,620,500, 7,620,502, 7,629,170, 7,702,464, 7,747,391, 7,747,393, 7,751,986, 7,776,598, 7,783,428, 7,795,030, 7,853,410, 7,868,138, 7,783,428, 7,873,477, 7,873,499, 7,904,249, 7,957,912, 7,981,614, 8,014,961, 8,029,988, 8,048,674, 8,058,001, 8,076,138, 8,108,150, 8,170,806, 8,224,580, 8,377,681, 8,383,346, 8,457,903, 8,504,498, 8,589,085, 8,762,066, 8,768,871, 9,593,326, 및 모든 관련 미국, PCT 및 미국 이외의 대응 특허; Ling et al., Anal. Biochem., 254(2):157-78 [1997]; Dale et al., Meth. Mol. Biol., 57:369-74 [1996]; Smith, Ann. Rev. Genet., 19:423-462 [1985]; Botstein et al., Science, 229:1193-1201 [1985]; Carter, Biochem. J., 237:1-7 [1986]; Kramer et al., Cell, 38:879-887 [1984]; Wells et al., Gene, 34:315-323 [1985]; Minshull et al., Curr. Op. Chem. Biol., 3:284-290 [1999]; Christians et al., Nat. Biotechnol., 17:259-264 [1999]; Crameri et al., Nature, 391:288-291 [1998]; Crameri, et al., Nat. Biotechnol., 15:436-438 [1997]; Zhang et al., Proc. Nat. Acad. Sci. U.S.A., 94:4504-4509 [1997]; Crameri et al., Nat. Biotechnol., 14:315-319 [1996]; Stemmer, Nature, 370:389-391 [1994]; Stemmer, Proc. Nat. Acad. Sci. USA, 91:10747-10751 [1994]; WO 95/22625; WO 97/0078; WO 97/35966; WO 98/27230; WO 00/42651; WO 01/75767; 및 WO 2009/152336 참조; 이들 모두는 본원에 참조로 포함됨).

일부 실시양태에서, 돌연변이 유발 처리 후 수득된 효소 클론은 효소 제제를 정의된 온도(또는 다른 분석 조건)에 적용하는 단계 및 열 처리 또는 다른 적합한 분석 조건 후 남아있는 효소 활성의 양을 측정하는 단계에 의해 스크리닝된다. 이후, 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 함유하는 클론을 유전자로부터 단리하고, 시퀀싱하여 뉴클레오타이드 서열 변화(존재하는 경우)를 확인하고, 숙주 세포에서 효소를 발현하는데 사용한다. 발현 라이브러리로부터 효소 활성을 측정하는 것은 관련 기술 분야에 공지된 임의의 적합한 방법(예를 들어, 표준 생화학 기술, 예를 들어 HPLC 분석)을 사용하여 수행될 수 있다.

변이체가 생성된 후, 이들은 임의의 원하는 특성(예를 들어, 높거나 증가된 활성, 또는 낮거나 감소된 활성, 증가된 열 활성, 증가된 열 안정성 및/또는 산성 pH 안정성 등)에 대해 스크리닝될 수 있다. 일부 실시양태에서, "재조합 포스포펜토뮤타제 폴리펩타이드"(본원에서 "조작된 포스포펜토뮤타제 폴리펩타이드", "변이체 포스포펜토뮤타제 효소", "포스포펜토뮤타제 변이체" 및 "포스포펜토뮤타제 조합 변이체"로도 지칭됨)가 유용하다. 일부 실시양태에서, "재조합 포스포펜토뮤타제 폴리펩타이드"("조작된 포스포펜토뮤타제 폴리펩타이드", "변이체 포스포펜토뮤타제 효소", "포스포펜토뮤타제 변이체" 및 "포스포펜토뮤타제 조합 변이체"로도 지칭됨)가 유용하다.

본원에서 사용되는 바와 같이, "벡터"는 DNA 서열을 세포에 도입하기 위한 DNA 구축물이다. 일부 실시양태에서, 벡터는 DNA 서열에서 코딩되는 폴리펩타이드의 적합한 숙주에서 발현을 수행할 수 있는 적합한 제어 서열에 작동 가능하게 연결된 발현 벡터이다. 일부 실시양태에서, "발현 벡터"는 숙주 세포에서 발현을 유도하기 위해 DNA 서열(예를 들어, 트랜스진)에 작동 가능하게 연결된 프로모터 서열을 갖고, 일부 실시양태에서 전사 종결자 서열도 포함한다.

본원에서 사용되는 바와 같이, 용어 "발현"은 전사, 전사후 변형, 번역 및 번역후 변형을 포함하지만 이로 제한되지 않는, 폴리펩타이드의 생산에 관련된 임의의 단계를 포함한다. 일부 실시양태에서, 상기 용어는 또한 세포로부터 폴리펩타이드의 분비를 포함한다.

본원에서 사용되는 바와 같이, 용어 "생산하다"는 세포에 의한 단백질 및/또는 다른 화합물의 생산을 지칭한다. 이 용어는 전사, 전사후 변형, 번역 및 번역후 변형을 포함하지만 이로 제한되지 않는, 폴리펩타이드의 생산에 관련된 임의의 단계를 포함하는 것으로 의도된다. 일부 실시양태에서, 상기 용어는 또한 세포로부터 폴리펩타이드의 분비를 포함한다.

본원에서 사용되는 바와 같이, 아미노산 또는 뉴클레오타이드 서열(예를 들어, 프로모터 서열, 신호 펩타이드, 종결자 서열 등)은 2개의 서열이 자연에서 회합되지 않는 경우, 그 서열이 작동 가능하게 연결된 또 다른 서열에 대해 "이종성"이다. 예를 들어, "이종성 폴리뉴클레오타이드"는 실험 기술에 의해 숙주 세포로 도입된 임의의 폴리뉴클레오타이드이며, 숙주 세포에서 제거되고 실험 조작을 거친 다음, 숙주 세포로 재도입되는 폴리뉴클레오타이드를 포함한다.

본원에서 사용되는 바와 같이, 용어 "숙주 세포" 및 "숙주 균주"는 본원에서 제공되는 DNA(예를 들어, 포스포펜토뮤타제 변이체를 코딩하는 폴리뉴클레오타이드)를 포함하는 발현 벡터에 적합한 숙주를 지칭한다. 일부 실시양태에서, 숙주 세포는 관련 기술 분야에 공지된 바와 같은 재조합 DNA 기술을 사용하여 구축된 벡터로 형질전환 또는 형질감염된 원핵 또는 진핵 세포이다.

용어 "유사체"는 기준 폴리펩타이드와 70% 초과의 서열 동일성 내지 100% 미만의 서열 동일성(예를 들어, 75%, 78%, 80%, 83%, 85%, 88%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 초과의 서열 동일성)을 갖는 폴리펩타이드를 의미한다. 일부 실시양태에서, 유사체는 호모아르기닌, 오르니틴 및 노르발린을 포함하지만 이로 제한되지 않는 하나 이상의 비자연 발생 아미노산 잔기뿐만 아니라, 자연 발생 아미노산을 함유하는 폴리펩타이드를 의미한다. 일부 실시양태에서, 유사체는 또한 하나 이상의 D-아미노산 잔기 및 2개 이상의 아미노산 잔기 사이에 비펩타이드 결합을 포함한다.

용어 "유효량"은 원하는 결과를 생성하는데 충분한 양을 의미한다. 관련 기술 분야에서 통상의 기술을 가진 자는 통상적인 실험을 사용하여 유효량을 결정할 수 있다.

용어 "단리된" 및 "정제된"은 자연적으로 회합되는 적어도 하나의 다른 성분으로부터 제거된 분자(예를 들어, 단리된 핵산, 폴리펩타이드 등) 또는 다른 성분을 지칭하기 위해 사용된다. 용어 "정제된"은 절대적인 순도를 요구하지 않으며, 오히려 상대적인 정의로서 의도된다.

본원에서 사용되는 바와 같이, "입체선택성"은 하나의 입체이성질체가 또 다른 입체이성질체보다 화학적 또는 효소에 의한 반응에서 우선적으로 형성되는 것을 지칭한다. 입체선택성은 하나의 입체이성질체의 형성이 다른 입체이성질체보다 선호되는 경우 부분적일 수 있거나, 또는 오직 하나의 입체이성질체가 형성되는 경우 완전할 수 있다. 입체이성질체가 거울상이성질체인 경우, 입체선택성은 두 거울상이성질체의 합에서 하나의 거울상이성질체의 분율(전형적으로 백분율로서 보고됨)인 거울상이성질체 선택성으로 지칭된다. 이것은 식 [주요 거울상이성질체 - 소수 거울상이성질체]/[주요 거울상이성질체 + 소수 거울상이성질체]에 따라 계산된 거울상이성질체 과량("e.e.")으로서 일반적으로 대안적으로 관련 기술 분야에 보고된다(전형적으로 백분율로서). 입체이성질체가 부분입체이성질체인 경우, 입체선택성은 일반적으로 대안적으로 부분입체이성질체 과량("d.e.")으로 보고되는, 2개의 부분입체이성질체의 혼합물에서 하나의 부분입체이성질체의 분율(전형적으로 백분율로서 보고됨)인 부분입체선택성으로 지칭된다. 거울상이성질체 과량 및 부분입체이성질체 과량은 입체이성질체 과량의 유형이다.

본원에서 사용되는 바와 같이, "위치선택성" 및 "위치선택적 반응"은 결합 형성 또는 절단에 대한 하나의 방향이 다른 모든 가능한 방향보다 우선적으로 발생하는 반응을 지칭한다. 반응은 구별이 완전하면 완전히(100%) 위치선택적일 수 있고, 하나의 부위에서의 반응 생성물이 다른 부위에서의 반응 생성물보다 우세한 경우 실질적으로 위치선택적(적어도 75%)이거나 부분적으로 위치선택적(X%, 여기서 백분율은 관심 반응에 의존적으로 설정됨)일 수 있다.

본원에서 사용되는 바와 같이, "화학선택성"은 화학적 또는 효소에 의한 반응에서 한 생성물이 다른 생성물에 비해 우선적으로 형성됨을 의미한다.

본원에서 사용되는 바와 같이, "pH 안정성"은 처리되지 않은 효소와 비교하여 일정 시간(예를 들어, 0.5-24시간) 동안 높은 또는 낮은 pH(예를 들어, 4.5-6 또는 8 내지 12)에 노출된 후, 유사한 활성(예를 들어, 60% 내지 80% 초과)을 유지하는 포스포펜토뮤타제 폴리펩타이드를 지칭한다.

본원에서 사용되는 바와 같이, "열안정성"은 동일한 승온에 노출된 야생형 효소와 비교하여 일정 시간(예를 들어, 0.5-24시간) 동안 승온(예를 들어, 40-80℃)에 노출된 후, 유사한 활성(예를 들어, 60% 내지 80% 초과)을 유지하는 포스포펜토뮤타제 폴리펩타이드를 지칭한다.

본원에서 사용되는 바와 같이, "용매 안정성"은 동일한 농도의 동일한 용매에 노출된 야생형 효소와 비교하여 일정 시간(예를 들어, 0.5-24시간) 동안 다양한 농도(예를 들어, 5-99%)의 용매(에탄올, 이소프로필 알코올, 디메틸설폭사이드 [DMSO], 테트라하이드로푸란, 2-메틸테트라하이드로푸란, 아세톤, 톨루엔, 부틸 아세테이트, 메틸 tert- 부틸 에테르 등)에 노출된 후, 유사한 활성(예를 들어, 60% 내지 80% 초과)을 유지하는 포스포펜토뮤타제 폴리펩타이드를 지칭한다.

본원에서 사용되는 바와 같이, "열 및 용매 안정성"은 열안정성이면서 용매 안정성인 포스포펜토뮤타제 폴리펩타이드를 지칭하는 것이다.

본원에서 사용되는 바와 같이, "선택적" 및 "선택적으로"는 추후 설명되는 사건 또는 상황이 발생하거나 발생하지 않을 수 있다는 것과, 상기 설명이 사건 또는 상황이 발생하는 경우 및 그것이 발생하지 않는 경우를 포함한다는 것을 의미한다. 관련 기술 분야에서 통상의 기술을 가진 자는 하나 이상의 선택적인 치환체를 함유하는 것으로 기재된 임의의 분자와 관련하여, 입체적으로 실제적이고/이거나 합성적으로 가능한 화합물만이 포함되는 것을 의미한다는 것을 이해할 것이다.

본원에서 사용되는 바와 같이, "선택적으로 치환된"은 화학적 기의 용어 또는 시리즈에서 모든 후속 변형자를 지칭한다. 예를 들어, "선택적으로 치환된 아릴알킬"이라는 용어에서, 분자의 "알킬" 부분 및 "아릴" 부분은 치환되거나 치환되지 않을 수 있으며, 일련의 "선택적으로 치환된 알킬, 사이클로알킬, 아릴 및 헤테로아릴"의 경우, 알킬, 사이클로알킬, 아릴 및 헤테로아릴 기는 다른 기와 독립적으로 치환되거나 치환되지 않을 수 있다.

발명의 상세한 설명

일부 실시양태에서, 본 발명은 MK-8591(Merck)과 같은 뉴클레오사이드 유사체의 생산에 적합한 효소를 제공한다. 본 발명은 이러한 뉴클레오사이드 유사체를 생산하기 위한 효소의 잠재적인 사용을 다루기 위해 개발되었다. 그러나, 이 접근법에 대한 한 가지 도전은 필요한 모든 중간체의 생산에 필요한 필수 기질 유사체에 대해 야생형 효소가 최적일 가능성이 없다는 것이다. 또한, 합성 경로에서 각각의 효소는 원하는 뉴클레오사이드 유사체의 합성에 사용되는 방법 및 대리 기질에 적합하도록 하기 위해 일부 조작을 필요로 한다.

일부 실시양태에서, 본 발명은 화합물 (1)로 나타낸 비-천연 뉴클레오사이드 유사체의 시험관내 효소 합성 방법을 궁극적으로 유도하는 화합물의 생산에 유용한 효소를 제공한다.

화합물 (1)

비-천연 뉴클레오사이드는 암 및 바이러스 감염의 치료를 위한 약물을 비롯한 많은 중요한 약물 클래스의 필수 구성 요소이다. 적어도 12개의 뉴클레오사이 유사 약물이 시판 중이거나 임상 시험 중에 있다(Jordheim et al., Nat. Rev. Drug Discovery 12:447-464 [2013]). 화합물 (1)을 제조하는 한 가지 방법은 반응식 I에 나타낸 바와 같이 에티닐 리보스-1-포스페이트, 화합물 (3), 및 플루오로아데닌, 화합물 (2)의 퓨린 뉴클레오사이드 포스포릴라제(PNP)에 의해 촉매된 커플링에 의한 것이다.

반응식 I

화합물 (3)과 같은 데옥시리보스-1-포스페이트 화합물은 제조하기 어려울 수 있다. 그러나, 상응하는 데옥시리보스-5-포스페이트 화합물은 효소 2-데옥시리보스-5-포스페이트 알돌라제(DERA)에 의해 촉매된 아세트알데하이드 및 D-글리세르알데하이드-3-포스페이트(또는 이들의 유사체)의 커플링을 통해 제조될 수 있다(Barbas et al., J. Am. Chem. Soc. 112:2013-2014 [1990]). 일단 데옥시리보스-5-포스페이트 유사체 (4)가 형성되면, 이것은 아래의 반응식 II에 제시된 바와 같이 효소 포스포펜토뮤타제(PPM)의 작용에 의해 반응식 I에 필요한 상응하는 데옥시리보스-1-포스페이트 유사체 (3)로 전환되거나 이성질화될 수 있다.

반응식 II. 포스포펜토뮤타제(PPM)에 의해 촉매된 반응

반응식 I에 제시된 PNP 및 PPM 반응의 평형 위치는 일반적으로 생성물(화합물 1 및 무기 포스페이트)이 아닌 반응물(화합물 2 및 4)에 유리하다. 반응을 더 높은 전환으로 유도하는 한 가지 방법은 커플링 단계에서 형성된 무기 포스페이트를 제거하는 것이다. 이는 효소 수크로스 포스포릴라제(SP)에 의해 촉매된, 수크로스와 같은 디사카라이드와 무기 포스페이트를 반응시킴으로써 달성될 수 있다(예를 들어, 미국 특허 제7,229,797호 참조). 글루코스-1-포스페이트 및 프럭토스를 생성하는 상기 반응은 매우 유리하며, 아래의 반응식 III에 제시된 바와 같이 전체 반응을 유도할 수 있다.

반응식 III. 화합물 (1)을 생성하기 위한 전체 반응식

포스포펜토뮤타제 효소는 특히 이. 콜라이(Barbas and Wong, Bioorg. Chem. 19:261-269 [1991]), 바실러스 세레우스(Bacillus cereus)(Panosian et al., Acta Crystallogr., Sect. F: Struct. Biol. Cryst. Commun., 66:811-814 [2010]), 바실러스 스파에리쿠스(Bacillus sphaericus)(Horinouchi et al., New Biotechnol. 26:75-82 [2009]), 및 사카로마이세스 세레비지애(Saccharomyces cerevisiae)(Walther et al., FEBS Lett. 586:4114-4118 [2012])를 포함하는 다수의 공급원으로부터 단리 및/또는 재조합적으로 발현되었다. 바실러스 세레우스로부터의 PPM의 결정 구조가 결정되었다(Panosian et al., Acta Crystallogr., Sect. F: Struct. Biol. Cryst. Commun., 66:811-814 [2010], Panosian et al., J. Biol. Chem. 286:8043-8054 [2011]). PPM은 평형에 유리한 위치가 (데옥시)리보스-5-포스페이트 측에 존재하면서 1-위치로부터 5-위치로의 (데옥시)리보스 상의 포스페이트 기의 상호전환을 촉매한다. 비-천연 리보스 포스페이트 유사체에 대한 PPM의 사용에 대한 설명은 문헌에서 제한되어 있다. 한 가지 예는 바실러스 스테아로테르모필루스(Bacillus stearothermophilus) PPM을 사용한 디데옥시리보스 5-포스페이트의 이성질화이다. 이 보고서에서, 효소는 상기 화합물에 대해 활성을 나타내지만, 활성은 리보스 5-포스페이트에 비해 12%에 불과하였다(Hamamoto et al., Biosci. Biotechnol., Biochem. 62:1103-1108 [1998]). 비-천연 기질에 대한 야생형 PPM의 불량한 활성은 화합물 (1)과 같은 비-천연 뉴클레오사이드의 생산에 대한 사용을 제한한다.

비-천연 및 치료상 유용한 뉴클레오사이드를 제조하기 위한 비-천연 기질에 대한 PPM의 불량한 활성으로 인해, 개선된 활성을 갖고 전형적인 산업 조건 하에서 작동할 수 있는 조작된 PPM에 대한 필요성이 존재한다. 본 발명은 이러한 요구를 해결하고, 산업 조건 하에서 이러한 반응에 사용하기 적합한 조작된 PPM을 제공한다.

조작된 PPM 폴리펩타이드

본 발명은 조작된 PPM 폴리펩타이드, 상기 폴리펩타이드를 코딩하는 폴리뉴클레오타이드, 상기 폴리펩타이드의 제조 방법 및 상기 폴리펩타이드의 사용 방법을 제공한다. 설명이 폴리펩타이드에 관한 경우, 그 설명은 폴리펩타이드를 코딩하는 폴리뉴클레오타이드도 설명하는 것으로 이해되어야 한다. 일부 실시양태에서, 본 발명은 야생형 PPM 효소에 비해 개선된 특성을 갖는 조작된 비-자연 발생 PPM 효소를 제공한다. 임의의 적합한 반응 조건이 본 발명에서 유용하다. 일부 실시양태에서, 이성질화 반응을 수행하기 위해 조작된 폴리펩타이드의 개선된 특성을 분석하기 위한 방법이 사용된다. 일부 실시양태에서, 반응 조건은 아래에서 및 실시에서 추가로 설명되는 바와 같이 조작된 PPM의 농도 또는 양, 기질(들), 완충제(들), 용매(들), pH, 온도 및 반응 시간을 포함하는 조건, 및/또는 고체 지지체 상에 고정된 조작된 PPM 폴리펩타이드에 대한 조건과 관련하여 변경된다.

일부 실시양태에서, 추가의 반응 성분 또는 추가의 기술이 반응 조건을 보충하기 위해 사용된다. 일부 실시양태에서, 이들은 효소를 안정화하거나 불활성화를 방지하고, 생성물 억제를 감소시키고, 반응 평형을 원하는 생성물 형성으로 이동시키기 위한 조치를 취하는 것을 포함한다.

일부 추가의 실시양태에서, 기질 화합물을 생성물 화합물로 전환하기 위한 상기 기재된 임의의 공정은 다음으로부터 선택된 하나 이상의 단계를 추가로 포함할 수 있다: 생성물 화합물(들)의 추출, 단리, 정제, 결정화, 여과 및/또는 동결건조. 본원에서 제공되는 공정에 의해 생산된 생촉매 반응 혼합물로부터 생성물(들)을 추출, 단리, 정제 및/또는 결정화하기 위한 방법, 기술 및 프로토콜은 통상의 기술자에게 공지되어 있고/있거나, 통상적인 실험을 통해 실시된다. 추가로, 예시적인 방법은 하기 실시예에서 제공된다.

조작된 폴리펩타이드를 코딩하는 조작된 PPM 폴리뉴클레오타이드, 발현 벡터 및 숙주 세포

본 발명은 본원에서 설명되는 조작된 효소 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 제공한다. 일부 실시양태에서, 폴리뉴클레오타이드는 폴리펩타이드를 발현할 수 있는 재조합 폴리뉴클레오타이드를 생성하기 위해 유전자 발현을 제어하는 하나 이상의 이종성 조절 서열에 작동 가능하게 연결될 수 있다. 일부 실시양태에서, 조작된 효소 폴리펩타이드(들)를 코딩하는 적어도 하나의 이종성 폴리뉴클레오타이드를 함유하는 발현 구축물은 상응하는 효소 폴리펩타이드(들)를 발현하기 위해 적절한 숙주 세포 내로 도입된다.

통상의 기술자에게 명백할 바와 같이, 단백질 서열의 이용 가능성 및 다양한 아미노산에 상응하는 코돈에 대한 지식은 대상 폴리펩타이드를 코딩할 수 있는 모든 폴리뉴클레오타이드에 대한 설명을 제공한다. 동일한 아미노산이 대체 또는 동의 코돈에 의해 코딩되는 유전자 코드의 축퇴성은 극히 많은 수의 핵산이 제조될 수 있도록 하며, 이들 모두는 조작된 효소(예를 들어, PPM) 폴리펩타이드를 코딩한다. 따라서, 본 발명은 가능한 코돈 선택에 기초하여 조합을 선택함으로써 본원에서 설명되는 효소 폴리펩타이드를 코딩하는 효소 폴리뉴클레오타이드의 각각의 및 모든 가능한 변이를 생성하기 위한 방법 및 조성물을 제공하고, 모든 상기 변이는 실시예에서(예를 들어, 다양한 표에서) 제시되는 아미노산 서열을 포함하는, 본원에서 설명되는 임의의 폴리펩타이드에 대해 구체적으로 개시된 것으로 간주될 것이다.

일부 실시양태에서, 코돈은 바람직하게는 단백질 생산을 위해 선택된 숙주 세포에 의한 이용을 위해 최적화된다. 예를 들어, 박테리아에서 사용되는 선호 코돈은 일반적으로 박테리아에서의 발현을 위해 사용된다. 결과적으로, 조작된 효소 폴리펩타이드를 코딩하는 코돈 최적화된 폴리뉴클레오타이드는 전장 코딩 영역에서 코돈 위치의 약 40%, 50%, 60%, 70%, 80%, 90% 또는 90% 초과의 위치에 바람직한 코돈을 포함한다.

일부 실시양태에서, 효소 폴리뉴클레오타이드는 본원에서 개시되는 특성을 갖는 효소 활성을 갖는 조작된 폴리펩타이드를 코딩하고, 여기서 폴리펩타이드는 본원에서 제공되는 서열 번호로부터 선택되는 기준 서열에 대해 적어도 60%, 65%, 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 동일성을 갖는 아미노산 서열, 또는 임의의 변이체의 아미노산 서열(예를 들어, 실시예에서 제공되는 변이체), 및 기준 폴리뉴클레오타이드(들)과 비교할 때 하나 이상의 잔기 차이, 또는 실시예에서 개시되는 임의의 변이체의 아미노산 서열(예를 들어, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10개 또는 그 초과의 아미노산 잔기 위치)을 포함한다. 일부 실시양태에서, 기준 폴리펩타이드 서열은 서열 번호 2, 4, 118, 266, 420, 562, 656, 790, 및/또는 846으로부터 선택된다.

일부 실시양태에서, 폴리뉴클레오타이드는 본원에서 제공되는 임의의 폴리뉴클레오타이드 서열, 또는 그의 상보체로부터 선택된 기준 폴리뉴클레오타이드 서열, 또는 본원에서 제공되는 임의의 변이체 효소 폴리펩타이드를 코딩하는 폴리뉴클레오타이드 서열에 매우 엄격한 조건 하에서 혼성화할 수 있다. 일부 실시양태에서, 매우 엄격한 조건 하에서 혼성화할 수 있는 폴리뉴클레오타이드는 기준 서열과 비교하여 하나 이상의 잔기 차이를 갖는 아미노산 서열을 포함하는 효소 폴리펩타이드를 코딩한다.

일부 실시양태에서, 본원에서 제공되는 임의의 조작된 효소 폴리펩타이드를 코딩하는 단리된 폴리뉴클레오타이드는 효소 폴리펩타이드의 발현을 촉진하기 위해 다양한 방식으로 조작된다. 일부 실시양태에서, 효소 폴리펩타이드를 코딩하는 폴리뉴클레오타이드는 하나 이상의 제어 서열이 효소 폴리뉴클레오타이드 및/또는 폴리펩타이드의 발현을 조절하기 위해 존재하는 발현 벡터를 포함한다. 단리된 폴리뉴클레오타이드를 벡터 내로 삽입하기 전에 조작하는 것은 이용되는 발현 벡터에 따라 바람직하거나 필요할 수 있다. 재조합 DNA 방법을 이용하여 폴리뉴클레오타이드 및 핵산 서열을 변형시키기 위한 기술은 관련 기술 분야에 널리 공지되어 있다. 일부 실시양태에서, 제어 서열은 다른 서열 중에서, 프로모터, 리더 서열, 폴리아데닐화 서열, 프로펩타이드 서열, 신호 펩타이드 서열, 및 전사 종결자를 포함한다. 일부 실시양태에서, 적합한 프로모터는 선택된 숙주 세포에 기초하여 선택될 수 있다. 박테리아 숙주 세포의 경우, 본 발명의 핵산 구축물의 전사를 지시하기 위한 적합한 프로모터는 이. 콜라이 lac 오페론, 스트렙토마이세스 코엘리컬러(Streptomyces coelicolor) 아가라아제 유전자(dagA), 바실러스 서브틸리스(Bacillus subtilis) 레반수크라아제 유전자(sacB), 바실러스 리케니포르미스(Bacillus licheniformis) 알파-아밀라아제 유전자(amyL), 바실러스 스테아로써모필러스(Bacillus stearothermophilus) 말토제닉(maltogenic) 아밀라아제 유전자(amyM), 바실러스 아밀로리퀘파시엔스(Bacillus amyloliquefaciens) 알파-아밀라아제 유전자(amyQ), 바실러스 리케니포르미스 페니실리나아제 유전자(penP), 바실러스 서브틸리스 xylA 및 xylB 유전자, 및 원핵 베타-락타마아제 유전자로부터 수득된 프로모터(예를 들어, 문헌 [Villa-Kamaroff et al., Proc. Natl Acad. Sci .USA 75: 3727-3731 [1978]] 참조)뿐만 아니라, tac 프로모터(예를 들어, 문헌 [DeBoer et al., Proc. Natl Acad. Sci. USA 80: 21-25 [1983] 참조)를 포함하지만, 이로 제한되지 않는다. 사상형 진균 숙주 세포를 위한 예시적인 프로모터는 아스퍼질러스 오리재(Aspergillus oryzae) TAKA 아밀라아제, 리조무코르 미에헤이(Rhizomucor miehei) 아스파르틱 프로테이나아제, 아스퍼질러스 니거(Aspergillus niger) 중성 알파-아밀라아제, 아스퍼질러스 니거 산 안정성 알파-아밀라아제, 아스퍼질러스 니거 또는 아스퍼질러스 아와모리(Aspergillus awamori) 글루코 아밀라아제(glaA), 리조무코르 미에헤이 리파아제, 아스퍼질러스 오리재 알칼리성 프로테아제, 아스퍼질러스 오리재 트리오스 포스페이트 이소머라아제, 아스퍼질러스 니둘란스(Aspergillus nidulans) 아세트아미다아제, 및 푸사리움 옥시스포룸(Fusarium oxysporum) 트립신-유사 프로테아제(예를 들어, WO 96/00787 참조)에 대한 유전자로부터 수득된 프로모터뿐만 아니라, NA2-tpi 프로모터(아스퍼질러스 니거 중성 알파-아밀라아제 및 아스퍼질러스 오리재 트리오스 포스페이트 이소머라아제에 대한 유전자로부터의 프로모터의 혼성체), 및 이의 돌연변이체 프로모터, 말단 절단된 프로모터, 및 혼성체 프로모터를 포함하지만, 이로 제한되지 않는다. 예시적인 효모 세포 프로모터는 사카로마이세스 세레비지애 에놀라아제(ENO-1), 사카로마이세스 세레비지애 갈락토키나아제(GAL1), 사카로마이세스 세레비지애 알코올 데히드로게나제/글리세르알데하이드-3-포스페이트 데히드로게나제(ADH2/GAP), 및 사카로마이세스 세레비지애 3-포스포글리세레이트 키나아제에 대한 유전자로부터 유래될 수 있다. 효모 숙주 세포를 위한 다른 유용한 프로모터는 관련 기술 분야에 공지되어 있다(예를 들어, 문헌 [Romanos et al., Yeast 8:423-488 [1992]] 참조).

일부 실시양태에서, 제어 서열은 적합한 전사 종결자 서열(즉, 전사를 종결시키기 위해 숙주 세포에 의해 인식되는 서열)이다. 일부 실시양태에서, 종결자 서열은 효소 폴리펩타이드를 코딩하는 핵산 서열의 3' 말단에 작동 가능하게 연결된다. 선택되는 숙주 세포에서 기능적인 임의의 적어도 종결자가 본 발명에서 사용된다. 사상형 진균 숙주 세포를 위한 예시적인 전사 종결자는 아스퍼질러스 오리재 TAKA 아밀라아제, 아스퍼질러스 니거 글루코아밀라아제, 아스퍼질러스 니둘란스 안트라닐레이트 합성효소, 아스퍼질러스 니거 알파-글루코시다아제, 및 푸사리움 옥시스포룸 트립신-유사 프로테아제에 대한 유전자로부터 수득될 수 있다. 효모 숙주 세포를 위한 예시적인 종결자는 사카로마이세스 세레비지애 에놀라아제, 사카로마이세스 세레비지애 시토크롬 C(CYC1), 및 사카로마이세스 세레비지애 글리세르알데하이드-3-포스페이트 데히드로게나제에 대한 유전자로부터 수득될 수 있다. 효모 숙주 세포를 위한 다른 유용한 종결자는 관련 기술 분야에 공지되어 있다(예를 들어, 문헌 [Romanos et al., 상기 문헌] 참조).

일부 실시양태에서, 제어 서열은 적합한 리더 서열(즉, 숙주 세포에 의한 번역에 중요한 mRNA의 비번역된 영역)이다. 일부 실시양태에서, 리더 서열은 효소 폴리펩타이드를 코딩하는 핵산 서열의 5' 말단에 작동 가능하게 연결된다. 선택된 숙주 세포에서 기능적인 임의의 적합한 리더 서열이 본 발명에서 사용될 수 있다. 사상형 진균 숙주 세포에 대한 예시적인 리더는 아스퍼질러스 오리재 TAKA 아밀라아제 및 아스퍼질러스 니둘란스 트리오스 포스페이트 이소머라아제에 대한 유전자로부터 수득된다. 효모 숙주 세포에 대한 적합한 리더는 사카로마이세스 세레비지애 에놀라아제(ENO-1), 사카로마이세스 세레비지애 3-포스포글리세레이트 키나아제, 사카로마이세스 세레비지애 알파-인자, 및 사카로마이세스 세레비지애 알코올 데히드로게나제/글리세르알데하이드-3-포스페이트 데히드로게나제(ADH2/GAP)에 대한 유전자로부터 수득된다.

일부 실시양태에서, 제어 서열은 폴리아데닐화 서열(즉, 핵산 서열의 3' 말단에 작동 가능하게 연결되고, 전사될 때 전사되는 mRNA에 폴리아데노신 잔기를 부가하기 위한 신호로서 숙주 세포에 의해 인식되는 서열)이다. 선택된 숙주 세포에서 기능적인 임의의 적합한 폴리아데닐화 서열이 본 발명에 사용될 수 있다. 사상형 진균 숙주 세포에 대한 예시적인 폴리아데닐화 서열은 아스퍼질러스 오리재 TAKA 아밀라아제, 아스퍼질러스 니거 글루코아밀라아제, 아스퍼질러스 니둘란스 안트라닐레이트 합성효소, 푸사리움 옥시스포룸 트립신-유사 프로테아제, 및 아스퍼질러스 니거 알파-글루코시다아제에 대한 유전자를 포함하지만, 이로 제한되지 않는다. 효모 숙주 세포에 대한 유용한 폴리아데닐화 서열은 공지되어 있다(예를 들어, 문헌 [Guo and Sherman, Mol. Cell. Bio., 15:5983-5990 [1995] 참조).

일부 실시양태에서, 제어 서열은 또한 신호 펩타이드(즉, 폴리펩타이드의 아미노 말단에 연결된 아미노산 서열을 코딩하고 코딩되는 폴리펩타이드를 세포의 분비 경로로 유도하는 코딩 영역)이다. 핵산 서열의 코딩 서열의 5' 말단은 분비된 폴리펩타이드를 코딩하는 코딩 영역의 세그먼트에 번역 리딩 프레임으로 자연적으로 연결된 신호 펩타이드 코딩 영역을 본질적으로 함유할 수 있다. 대안적으로, 일부 실시양태에서, 코딩 서열의 5' 말단은 코딩 서열에 대해 외래 물질인 신호 펩타이드 코딩 영역을 함유한다. 발현된 폴리펩타이드를 선택된 숙주 세포의 분비 경로 내로 유도하는 임의의 적합한 신호 펩타이드 코딩 영역이 조작된 폴리펩타이드의 발현에 사용될 수 있다. 박테리아 숙주 세포에 대한 효과적인 신호 펩타이드 코딩 영역은 바실러스 NClB 11837 말토제닉 아밀라아제, 바실러스 스테아로써모필러스 알파-아밀라아제, 바실러스 리케니포르미스 서브틸리신, 바실러스 리케니포르미스 베타-락타마아제, 바실러스 스테아로써모필러스 중성 프로테아제(nprT, nprS, nprM), 및 바실러스 서브틸리스 prsA에 대한 유전자로부터 수득된 것을 포함하지만 이로 제한되지 않는 신호 펩타이드 코딩 영역이다. 추가의 신호 펩타이드가 관련 기술 분야에 공지되어 있다(예를 들어, 문헌 [Simonen and Palva, Microbiol. Rev., 57:109-137 [1993] 참조). 일부 실시양태에서, 사상형 진균 숙주 세포에 대한 효과적인 신호 펩타이드 코딩 영역은 아스퍼질러스 오리재 TAKA 아밀라아제, 아스퍼질러스 니거 중성 아밀라아제, 아스퍼질러스 니거 글루코아밀라아제, 리조무코르 미에헤이 아스파르틱 프로테이나아제, 후미콜라 인솔렌스(Humicola insolens) 셀룰라아제, 및 후미콜라 라누기노사(Humicola lanuginosa) 리파아제에 대한 유전자로부터 수득된 신호 펩타이드 코딩 영역을 포함하지만, 이로 제한되지 않는다. 효모 숙주 세포에 대한 유용한 신호 펩타이드는 사카로마이세스 세레비지애 알파-인자 및 사카로마이세스 세레비지애 인버타아제에 대한 유전자로부터의 것을 포함하지만, 이로 제한되지 않는다.

일부 실시양태에서, 제어 서열은 폴리펩타이드의 아미노 말단에 위치한 아미노산 서열을 코딩하는 프로펩타이드 코딩 영역이다. 생성된 폴리펩타이드는 "프로효소", "프로폴리펩타이드" 또는 "자이모겐(zymogen)"으로 지칭된다. 프로폴리펩타이드는 프로폴리펩타이드로부터 프로펩타이드의 촉매에 의한 또는 자가촉매에 의한 절단에 의해 성숙한 활성 폴리펩타이드로 전환될 수 있다. 프로펩타이드 코딩 영역은 바실러스 서브틸리스 알칼리성 프로테아제(aprE), 바실러스 서브틸리스 중성 프로테아제(nprT), 사카로마이세스 세레비지애 알파-인자, 리조무코르 미에헤이 아스파르틱 프로티나아제, 및 미셀리오프토라 써모필라(Myceliophthora thermophila) 락타아제에 대한 유전자를 포함하지만, 이로 제한되지 않는다(예를 들어, WO 95/33836 참조). 신호 펩타이드 및 프로펩타이드 영역 모두가 폴리펩타이드의 아미노 말단에 존재하는 경우, 프로펩타이드 영역은 폴리펩타이드의 아미노 말단 다음에 위치하고, 신호 펩타이드 영역은 프로펩타이드 영역의 아미노 말단 다음에 위치한다.

일부 실시양태에서, 조절 서열이 또한 이용된다. 이들 서열은 숙주 세포의 성장과 관련하여 폴리펩타이드의 발현의 조절을 용이하게 한다. 조절 시스템의 예는 유전자의 발현이 조절 화합물의 존재를 포함하는 화학적 또는 물리적 자극에 반응하여 켜지거나 꺼지게 하는 것이다. 원핵 숙주 세포에서, 적합한 조절 서열은 lac, tac, 및 trp 오퍼레이터(operator) 시스템을 포함하지만, 이로 제한되지 않는다. 효모 숙주 세포에서, 적합한 조절 시스템은 ADH2 시스템 또는 GAL1 시스템을 포함하지만, 이로 제한되지 않는다. 사상형 진균에서, 적합한 조절 서열은 TAKA 알파-아밀라아제 프로모터, 아스퍼질러스 니거 글루코아밀라아제 프로모터, 및 아스퍼질러스 오리재 글루코아밀라아제 프로모터를 포함하지만, 이로 제한되지 않는다.

또 다른 측면에서, 본 발명은 조작된 효소 폴리펩타이드를 코딩하는 폴리뉴클레오타이드, 및 이들이 도입되는 숙주의 유형에 따라 하나 이상의 발현 조절 영역, 예를 들어 프로모터 및 종결자, 복제 기점 등을 포함하는 재조합 발현 벡터에 관한 것이다. 일부 실시양태에서, 본원에서 설명되는 다양한 핵산 및 제어 서열은 함께 연결되어, 하나 이상의 편리한 제한 부위에 효소 폴리펩타이드를 코딩하는 핵산 서열의 삽입 또는 치환을 허용하는 상기 제한 부위를 포함하는 재조합 발현 벡터를 생산한다. 대안적으로, 일부 실시양태에서, 본 발명의 핵산 서열은 핵산 서열 또는 상기 서열을 포함하는 핵산 구축물을 발현을 위한 적절한 벡터 내로 삽입함으로써 발현된다. 발현 벡터의 생성을 수반하는 일부 실시양태에서, 코딩 서열은 발현을 위한 적절한 제어 서열과 작동 가능하게 연결되도록 벡터에 위치한다.

재조합 발현 벡터는 재조합 DNA 절차를 편리하게 거칠 수 있고 효소 폴리뉴클레오타이드 서열의 발현을 유도할 수 있는 임의의 적합한 벡터(예를 들어, 플라스미드 또는 바이러스)일 수 있다. 벡터의 선택은 전형적으로 벡터가 도입되는 숙주 세포에 대한 벡터의 적합성에 따라 결정될 것이다. 벡터는 선형 또는 폐쇄 원형 플라스미드일 수 있다.

일부 실시양태에서, 발현 벡터는 자율 복제 벡터(즉, 플라스미드, 염색체외 요소, 미니염색체, 또는 인공 염색체와 같이, 그의 복제가 염색체 복제와는 무관한 염색체외 실체로서 존재하는 벡터)이다. 벡터는 자가 복제를 보장하기 위한 임의의 수단을 함유할 수 있다. 일부 대안적인 실시양태에서, 벡터는 숙주 세포 내로 도입될 때 게놈 내로 통합되고 그것이 통합된 염색체(들)와 함께 복제되는 벡터이다. 또한, 일부 실시양태에서, 숙주 세포의 게놈 내로 도입되는 총 DNA를 함께 함유하는 단일 벡터 또는 플라스미드 또는 2개 이상의 벡터 또는 플라스미드, 또는 트랜스포존이 사용될 수 있다.

일부 실시양태에서, 발현 벡터는 형질전환된 세포의 쉬운 선별을 허용하는 하나 이상의 선별 가능 마커를 함유한다. "선별 가능 마커"는 그의 생성물이 살생물제 또는 바이러스 내성, 중금속에 대한 내성, 영양요구성(auxotrophy)에 대한 원영양성(prototrophy) 등을 제공하는 유전자이다. 박테리아 선별 가능 마커의 예는 바실러스 서브틸리스 또는 바실러스 리케니포르미스로부터의 dal 유전자, 또는 암피실린, 카나마이신, 클로람페니콜 또는 테트라사이클린 내성과 같은 항생제 내성을 부여하는 마커를 포함하지만, 이로 제한되지 않는다. 효모 숙주 세포를 위한 적합한 마커는 ADE2, HIS3, LEU2, LYS2, MET3, TRP1, 및 URA3을 포함한다. 사상형 진균 숙주 세포에서 사용하기 위한 선별 가능 마커는 amdS(아세트아미다아제; 예를 들어 에이. 니둘란스 또는 에이. 오리재로부터의 것), argB(오르니틴 카르바모일트랜스퍼라제), bar(포스피노트리신 아세틸트랜스퍼라제; 예를 들어 에스. 하이그로스코피쿠스(S. hygroscopicus)), hph(하이그로마이신 포스포트랜스퍼라제), niaD(니트레이트 리덕타제), pyrG(오로티딘-5'-포스페이트 데카르복실라아제; 예를 들어 에이. 니둘란스 또는 에이. 오리재로부터의 것), sC(설페이트 아데닐트랜스퍼라제), 및 trpC(안트라닐레이트 합성효소)뿐만 아니라, 이들의 등가물을 포함하지만, 이로 제한되지 않는다.

또 다른 측면에서, 본 발명은 본 발명의 적어도 하나의 효소 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 포함하는 숙주 세포를 제공하며, 상기 폴리뉴클레오타이드(들)는 숙주 세포에서 조작된 효소(들)의 발현을 위해 하나 이상의 제어 서열에 작동 가능하게 결합된다. 본 발명의 발현 벡터에 의해 코딩되는 폴리펩타이드를 발현하는데 사용하기 위한 적합한 숙주 세포는 관련 기술 분야에 널리 알려져 있으며, 박테리아 세포, 예를 들어 이. 콜라이, 비브리오 플루비알리스(Vibrio fluvialis), 스트렙토마이세스 및 살모넬라 티피무리움(Salmonella typhimurium) 세포; 진균 세포, 예를 들어 효모 세포(예를 들어, 사카로마이세스 세레비지애 및 피키아 파스토리스(Pichia pastoris) [ATCC 수탁 번호 201178]); 곤충 세포, 예를 들어 초파리(Drosophila) S2 및 스포도프테라(Spodoptera) Sf9 세포; 동물 세포, 예를 들어 CHO, COS, BHK, 293, 및 보우스 흑색종(Bowes melanoma) 세포; 및 식물 세포를 포함하지만, 이로 제한되지 않는다. 예시적인 숙주 세포는 에셔리키아 콜라이 균주(예를 들어, W3110(△fhuA) 및 BL21)이다. 박테리아 선별 가능 마커의 예는 바실러스 서브틸리스 또는 바실러스 리케니포르미스로부터의 dal 유전자, 또는 암피실린, 카나마이신, 클로람페니콜 및/또는 테트라사이클린 내성과 같은 항생제 내성을 부여하는 마커를 포함하지만, 이로 제한되지 않는다.

일부 실시양태에서, 본 발명의 발현 벡터는 벡터를 숙주 세포의 게놈 내로의 통합 또는 게놈과 무관한 세포 내에서의 벡터의 자율 복제를 허용하는 요소(들)를 함유한다. 숙주 세포 게놈 내로의 통합을 포함하는 일부 실시양태에서, 벡터는 폴리펩타이드를 코딩하는 핵산 서열 또는 상동성 또는 비상동성 재조합에 의해 벡터를 게놈 내로 통합하기 위한 벡터의 임의의 다른 요소에 의존한다.

일부 대안적 실시양태에서, 발현 벡터는 상동성 재조합에 의해 숙주 세포를 게놈 내로 통합하도록 지시하기 위한 추가의 핵산 서열을 포함한다. 추가의 핵산 서열은 벡터가 염색체(들) 내의 정확한 위치(들)에서 숙주 세포 게놈 내로 통합될 수 있게 한다. 정확한 위치에서 통합 가능성을 높이기 위해, 통합 요소는 바람직하게는 100 내지 10,000개의 염기쌍, 바람직하게는 400 내지 10,000개의 염기쌍, 가장 바람직하게는 800 내지 10,000개의 염기쌍과 같은 충분한 수의 뉴클레오타이드를 포함하며, 이는 상동성 재조합 확률을 높이기 위해 상응하는 표적 서열에 대한 상동성이 높다. 통합 요소는 숙주 세포의 게놈 내의 표적 서열에 대해 상동성인 임의의 서열일 수 있다. 더욱이, 통합 요소는 핵산 서열을 코딩하지 않거나 코딩할 수 있다. 다른 한편으로, 벡터는 비-상동성 재조합에 의해 숙주 세포의 게놈 내에 통합될 수 있다.

자율 복제의 경우, 벡터는 벡터가 해당 숙주 세포에서 자율적으로 복제할 수 있도록 하는 복제 기점을 추가로 포함할 수 있다. 박테리아 복제 기점의 예는 P15A ori 또는 플라스미드 pBR322, pUC19, pACYC177(플라스미드가 P15A ori를 가짐) 또는 이. 콜라이에서 복제를 허용하는 pACYC184 및 바실러스에서 복제를 허용하는 pUB110, pE194 또는 pTA1060의 복제 기점이다. 효모 숙주 세포에서 사용하기 위한 복제 기점의 예는 2 미크론 복제 기점, ARS1, ARS4, ARS1과 CEN3의 조합, ARS4와 CEN6의 조합이다. 복제 기점은 그 기능을 숙주 세포에서 온도에 민감하게 만드는 돌연변이를 갖는 것일 수 있다(예를 들어, 문헌 [Ehrlich, Proc. Natl. Acad. Sci. USA 75:1433 [1978] 참조).

일부 실시양태에서, 본 발명의 핵산 서열의 하나 초과의 카피가 숙주 세포에 삽입되어 유전자 산물의 생산을 증가시킨다. 핵산 서열의 카피 수의 증가는 서열의 적어도 하나의 추가의 카피를 숙주 세포 게놈에 통합하거나 또는 증폭 가능한 선별 가능 마커 유전자를 핵산 서열과 함께 포함시킴으로써 얻을 수 있으며, 여기서 선별 가능한 마커 유전자의 증폭된 카피, 및 이에 따라 핵산 서열의 추가의 카피를 함유하는 세포는 적절한 선별 가능 제제의 존재 하에 세포를 배양함으로써 선별될 수 있다.

본 발명에서 사용하기 위한 많은 발현 벡터는 상업적으로 이용 가능하다. 적합한 상업적 발현 벡터는 포유동물 숙주 세포에서의 발현을 위한 CMV 프로모터 및 hGH 폴리아데닐화 부위 및 이. 콜라이에서의 증폭을 위한 pBR322 복제 기점 및 암피실린 내성 마커를 포함하는 p3xFLAGTM™ 발현 벡터(Sigma-Aldrich Chemicals)를 포함하지만, 이로 제한되지 않는다. 다른 적합한 발현 벡터는 pBluescriptII SK(-) 및 pBK-CMV(Stratagene), 및 pBR322(Gibco BRL), pUC(Gibco BRL), pREP4, pCEP4(Invitrogen) 또는 pPoly에서 유래된 플라스미드를 포함하지만, 이로 제한되지 않는다(예를 들어, 문헌 [Lathe et al., Gene 57:193-201 [1987]] 참조).

따라서, 일부 실시양태에서, 적어도 하나의 변이체 포스포펜토뮤타제를 코딩하는 서열을 포함하는 벡터는 벡터의 증식 및 변이체 포스포펜토뮤타제(들)의 발현을 허용하기 위해 숙주 세포로 형질전환된다. 일부 실시양태에서, 변이체 포스포펜토뮤타제는 번역 후에 변형되어 신호 펩타이드를 제거하고, 일부 경우에 분비 후에 절단될 수 있다. 일부 실시양태에서, 상기 설명된 형질전환된 숙주 세포는 변이체 포스포펜토뮤타제(들)의 발현을 허용하는 조건 하에 적합한 영양 배지에서 배양된다. 적절한 보충제를 함유하는 최소 또는 복합 배지를 포함하지만 이로 제한되지 않는, 숙주 세포 배양에 유용한 임의의 적합한 배지가 본 발명에서 유용하다. 일부 실시양태에서, 숙주 세포는 HTP 배지에서 성장된다. 적절한 배지는 다양한 상업적 공급 업체에서 구할 수 있거나, 발표된 제조 방법(예를 들어, American Type Culture Collection의 카탈로그)에 따라 준비할 수 있다.

또 다른 측면에서, 본 발명은 본원에서 제공되는 개선된 포스포펜토뮤타제 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 포함하는 숙주 세포를 제공하며, 상기 폴리뉴클레오타이드는 숙주 세포에서 포스포펜토뮤타제 효소의 발현을 위해 하나 이상의 제어 서열에 작동 가능하게 연결된다. 본 발명의 발현 벡터에 의해 코딩되는 포스포펜토뮤타제 폴리펩타이드를 발현하는데 사용하기 위한 숙주 세포는 관련 기술 분야에 잘 알려져 있으며, 박테리아 세포, 예를 들어 이. 콜라이, 바실러스 메가테리움(Bacillus megaterium), 락토바실러스 케피르( Lactobacillus kefir), 스트렙토마이세스 및 살모넬라 티피무리움 세포; 진균 세포, 예를 들어, 효모 세포(예를 들어, 사카로마이세스 세레비지애 또는 피키아 파스토리스(ATCC 수탁 번호 201178)); 곤충 세포, 예를 들어 초파리 S2 및 스포도프테라 Sf9 세포; 동물 세포, 예를 들어 CHO, COS, BHK, 293 및 보우스 흑색종 세포; 및 식물 세포를 포함하지만, 이로 제한되지 않는다. 상기 설명한 숙주 세포에 대한 적절한 배양 배지 및 성장 조건은 관련 기술 분야에 잘 알려져 있다.

포스포펜토뮤타제의 발현을 위한 폴리뉴클레오타이드는 관련 기술 분야에 공지된 다양한 방법에 의해 세포 내로 도입될 수 있다. 기술에는 전기천공, 바이올리스틱(biolistic) 입자 충격, 리포좀 매개 형질감염, 염화칼슘 형질감염 및 원형질체 융합이 포함된다. 폴리뉴클레오타이드를 세포 내로 도입하기 위한 다양한 방법이 관련 기술 분야에서 통상의 기술을 가진 자에게 공지되어 있다.

일부 실시양태에서, 숙주 세포는 진핵 세포이다. 적합한 진핵 숙주 세포는 진균 세포, 조류 세포, 곤충 세포 및 식물 세포를 포함하지만, 이로 제한되지 않는다. 적합한 진균 숙주 세포는 아스코미코타(Ascomycota), 바시디오미코타(Basidiomycota), 듀테로미코타(Deuteromycota), 자이고미코타(Zygomycota), 펑가이 임페르펙티(Fungi imperfecti)를 포함하지만, 이로 제한되지 않는다. 일부 실시양태에서, 진균 숙주 세포는 효모 세포 및 사상형 진균 세포이다. 본 발명의 사상형 진균 숙주 세포는 아문(subdivision) 유미코티나(Eumycotina) 및 오오미코타(Oomycota)의 모든 사상형 형태를 포함한다. 사상형 진균은 키틴, 셀룰로스 및 기타 복잡한 폴리사카라이드로 이루어진 세포벽을 가진 영양 균사체를 특징으로 한다. 본 발명의 사상형 진균 숙주 세포는 형태학적으로 효모와 구별된다.

본 발명의 일부 실시양태에서, 사상형 숙주 세포는 아클리아(Achlya), 아크레모늄(Acremonium), 아스퍼질러스, 아우레오바시디움(Aureobasidium), 브저르칸데라(Bjerkandera), 세리포리옵시스(Ceriporiopsis), 세팔로스포리움(Cephalosporium), 크리소스포리움(Chrysosporium), 코클리오볼루스(Cochliobolus), 코리나스쿠스(Corynascus), 크로포넥트리아(Cryphonectria), 크립토코커스(Cryptococcus), 코프리누스(Coprinus), 콜리올루스(Coriolus), 디플로디아(Diplodia), 엔도티스(Endothis), 푸사리움(Fusarium), 지베렐라(Gibberella), 글리오클라디움(Gliocladium), 휴미콜라(Humicola), 하이포크레아(Hypocrea), 마이셀리오프토라(Myceliophthora), 무코르(Mucor), 뉴로스포라(Neurospora), 페니실리움(Penicillium), 포도스포라(Podospora), 플레비아(Phlebia), 피로마이세스(Piromyces), 피리쿨라리아(Pyricularia), 리조무코르(Rhizomucor), 리조푸스(Rhizopus), 쉬조필룸(Schizophyllum), 스키탈리디움(Scytalidium), 스포로트리쿰(Sporotrichum), 탈라로마이세스(Talaromyces), 써모아스쿠스(Thermoascus), 티엘라비아(Thielavia), 트라메테스(Trametes), 톨리포클라디움(Tolypocladium), 트리코데르마(Trichoderma), 베르티실리움(Verticillium), 및/또는 볼바리엘라(Volvariella), 및/또는 이들의 유성생식형, 또는 무성생식형 및 동의어, 기본명(basionym) 또는 분류학적 동등물을 포함하지만 이로 제한되지 않는 임의의 적합한 속 및 종의 숙주 세포이다.

본 발명의 일부 실시양태에서, 숙주 세포는 칸디다(Candida), 한세눌라(Hansenula), 사카로마이세스, 쉬조사카로마이세스(Schizosaccharomyces), 피키아, 클루이베로마이세스(Kluyveromyces), 또는 야로위아(Yarrowia) 종의 세포를 포함하지만 이로 제한되지 않는 효모 세포이다. 본 발명의 일부 실시양태에서, 효모 세포는 한세눌라 폴리모르파(Hansenula polymorpha), 사카로마이세스 세레비지애, 사카로마이세스 카를스페르겐시스(Saccharomyces carlsbergensis), 사카로마이세스 디아스타티쿠스(Saccharomyces diastaticus), 사카로마이세스 노르벤시스(Saccharomyces norbensis), 사카로마이세스 클루이베리(Saccharomyces kluyveri), 쉬조사카로마이세스 폼베(Schizosaccharomyces pombe), 피키아 파스토리스, 피키아 핀란디카(Pichia finlandica), 피키아 트레할로필라(Pichia trehalophila), 피키아코다마에(Pichia kodamae), 피키아 멤브라나에파시엔스(Pichia membranaefaciens), 피키아 오푼티아에(Pichia opuntiae), 피키아 써모톨레란스(Pichia thermotolerans), 피키아 살릭타리아(Pichia salictaria), 피키아 퀘르쿠움(Pichia quercuum), 피키아 피이페리(Pichia pijperi), 피키아 스티피티스(Pichia stipitis), 피키아 메타놀리카(Pichia methanolica), 피키아 안구스타(Pichia angusta), 클루이베로마이세스 락티스(Kluyveromyces lactis), 칸디다 알비칸스(Candida albicans), 또는 야로위아 리폴리티카(Yarrowia lipolytica)이다.

본 발명의 일부 실시양태에서, 숙주 세포는 조류 세포, 예를 들어 클라미도모나스(Chlamydomonas)(예를 들어, 씨. 레인하르드티이(C. reinhardtii)) 및 포르미디움(Phormidium)(피. 종 ATCC29409)이다.

일부 다른 실시양태에서, 숙주 세포는 원핵 세포이다. 적합한 원핵 세포는 그람 양성, 그람 음성 및 그람 염색 가변성(Gram-variable) 박테리아 세포를 포함하지만, 이로 제한되지 않는다. 다음을 포함하지만 이로 제한되지 않는 임의의 적합한 박테리아 유기체가 본 발명에서 유용하다: 아그로박테리움(Agrobacterium), 알리시클로바실러스(Alicyclobacillus), 아나바에나(Anabaena), 아나시스티스(Anacystis), 아시네토박터(Acinetobacter), 아시도써무스(Acidothermus), 아르트로박터(Arthrobacter), 아조박터(Azobacter), 바실러스, 비피도박테리움(Bifidobacterium), 브레비박테리움(Brevibacterium), 부티리비브릭(Butyrivibrio), 부크네라(Buchnera), 캄페스트리스(Campestris), 캄플리오박터(Camplyobacter), 클로스트리디움(Clostridium), 코리네박테리움(Corynebacterium), 크로마티움(Chromatium), 코프로코쿠스(Coprococcus), 에셔리키아, 엔테로코쿠스(Enterococcus), 엔테로박터(Enterobacter), 에르위니아(Erwinia), 푸소박테리움(Fusobacterium), 파에칼리박테리움(Faecalibacterium), 프란시셀라(Francisella), 플라보박테리움(Flavobacterium), 게오바실러스(Geobacillus), 해모필루스(Haemophilus), 헬리코박터(Helicobacter), 클렙시엘라(Klebsiella), 락토바실러스(Lactobacillus), 락토코쿠스(Lactococcus), 일리오박터(Ilyobacter), 미크로코쿠스(Micrococcus), 미크로박테리움(Microbacterium), 메소리조븀(Mesorhizobium), 메틸로박테리움(Methylobacterium), 메틸로박테리움, 미코박테리움(Mycobacterium), 네이세리아(Neisseria), 판토에아(Pantoea), 슈도모나스(Pseudomonas), 프로클로로코쿠스(Prochlorococcus), 도도박터(Rhodobacter), 로도슈도모나스(Rhodopseudomonas), 로도슈도모나스, 로세부리아(Roseburia), 로도스피릴룸(Rhodospirillum), 로도코쿠스(Rhodococcus), 세네데스무스(Scenedesmus), 스트렙토마이세스, 스트렙토코쿠스(Streptococcus), 시네코쿠스(Synecoccus), 사카로모노스포라(Saccharomonospora), 스타필로코쿠스(Staphylococcus), 세라티아(Serratia), 살모넬라(Salmonella), 쉬겔라(Shigella), 써모아나에로박테리움(Thermoanaerobacterium), 트로페리마(Tropheryma), 툴라렌시스(Tularensis), 테메쿨라(Temecula), 써모시네코코쿠스(Thermosynechococcus), 써모코쿠스(Thermococcus), 우레아플라스마(Ureaplasma),잔토모나스(Xanthomonas), 자일렐라(Xylella), 예르시니아(Yersinia) 및 자이모모나스(Zymomonas). 일부 실시양태에서, 숙주 세포는 아그로박테리움, 아시네토박터, 아조박터, 바실러스, 비피도박테리움, 부크네라, 게오바실러스, 캄필로박터, 클로스트리듐, 코리네박테리움, 에셔리키아, 엔테로코쿠스, 에르위니아, 플라보박테리움, 락토바실러스, 락토코쿠스, 판토에아, 슈도모나스, 스타필로코쿠스, 살모넬라, 스트렙토코쿠스, 스트렙토마이세스, 또는 자이모모나스의 종이다. 일부 실시양태에서, 박테리아 숙주 균주는 인간에게 비병원성이다. 일부 실시양태에서, 박테리아 숙주 균주는 산업용 균주이다. 많은 박테리아 산업용 균주가 알려져 있고, 본 발명에 적합하다. 본 발명의 일부 실시양태에서, 박테리아 숙주 세포는 아그로박테리움 종(예를 들어, 에이. 라디오박터(A. radiobacter), 에이. 리조게네스(A. rhizogenes), 및 에이. 루비(A. rubi))이다. 본 발명의 일부 실시양태에서, 박테리아 숙주 세포는 아르트로박터(Arthrobacter) 종(예를 들어, 에이. 아우레센스(A. aurescens), 에이. 시트레우스(A. citreus), 에이. 글로비포르미스(A. globiformis), 에이. 히드로카르보글루타미쿠스(A. hydrocarboglutamicus), 에이. 미소렌스(A. mysorens), 에이. 니코티아나에(A. nicotianae), 에이. 파라피네우스(A. paraffineus), 에이. 프로토포니아에(A. protophonniae), 에이. 로세오파르크피누스(A. roseoparqffinus), 에이. 술푸레우스(A. sulfureus), 및 에이. 우레아파시엔스(A. ureafaciens)이다. 본 발명의 일부 실시양태에서, 박테리아 숙주 세포는 바실러스 종(예를 들어, 비. 투링겐시스(B. thuringensis), 비. 안트라시스(B. anthracis), 비. 메가테리움(B. megaterium), 비. 서브틸리스(B. subtilis), 비. 렌투스(B. lentus), 비. 시르쿨란스(B. circulans), 비. 푸밀루스(B. pumilus), 비. 라우투스(B. lautus), 비. 코아굴란스(B.coagulans), 비. 브레비스(B. brevis), 비. 피르무스(B. firmus), 비. 알카오피우스(B. alkaophius), 비. 리케니포르무스(B. licheniformis), 비. 클라우시이(B. clausii), 비. 스테아로써모필루스(B. stearothermophilus), 비. 할로두란스(B. halodurans), 및 비. 아밀로리퀘파시엔스(B. amyloliquefaciens)이다. 일부 실시양태에서, 숙주 세포는 비. 서브틸리스, 비. 푸밀루스, 비. 리케니포르무스, 비. 메가테리움, 비. 클라우시이, 비. 스테아로써모필루스 또는 비. 아밀로리퀘파시엔스를 포함하지만 이로 제한되지 않는 산업용 바실러스 균주이다. 일부 실시양태에서, 바실러스 숙주 세포는 비. 서브틸리스, 비. 리케니포르무스, 비. 메가테리움, 비. 스테아로써모필루스 및/또는 비. 아밀로리퀘파시엔스이다. 일부 실시양태에서, 박테리아 숙주 세포는 클로스트리디움 종(예를 들어, 씨. 아세토부틸리쿰(C. acetobutylicum), 씨. 테타니(C. tetani E88), 씨. 리투세부렌세(C. lituseburense), 씨. 사카로부틸리쿰(C. saccharobutylicum), 씨. 페르프링겐스(C. perfringens), 및 씨. 베이제링키이(C. beijerinckii)이다. 일부 실시양태에서, 박테리아 숙주 세포는 코리네박테리움 종(예를 들어, 씨. 글루타미쿰(C. glutamicum) 및 씨. 아세토아시도필룸(C. acetoacidophilum)이다. 일부 실시양태에서, 박테리아 숙주 세포는 에셔리키아 종(예를 들어, 이. 콜라이)이다. 일부 실시양태에서, 숙주 세포는 에셔리키아 콜라이 W3110이다. 일부 실시양태에서, 박테리아 숙주 세포는 에르위니아 종(예를 들어, 이. 우레도보라(E. uredovora), 이. 카로토보라(E. carotovora), 이. 아나나스(E. ananas), 이. 헤르비콜라(E. herbicola), 이. 푼크타타(E. punctata), 및 이. 테레우스(E. terreus)이다. 일부 실시양태에서, 박테리아 숙주 세포는 판토에아 종(예를 들어, 피. 시트레아(P. citrea) 및 피. 아글로메란스(P. agglomerans))이다. 일부 실시양태에서, 박테리아 숙주 세포는 슈도모나스 종(예를 들어, 피. 푸티다(P. putida), 피. 아에루기노사(P. aeruginosa), 피. 메발로니이이(P. mevalonii), 및 피. 종 D-01 10)이다. 일부 실시양태에서, 박테리아 숙주 세포는 스트렙토코쿠스 종(예를 들어, 에스. 에퀴시밀레스(S. equisimiles), 에스. 피오게네스(S. pyogenes), 및 에스. 우베리스(S. uberis))이다. 일부 실시양태에서, 박테리아 숙주 세포는 스트렙토마이세스 종(예를 들어, 에스. 암보파시엔스(S. ambofaciens), 에스. 아크로모게네스(S. achromogenes), 에스. 아베르미틸리스(S. avermitilis), 에스. 코엘리콜로르(S. coelicolor), 에스. 아우레오파시엔스(S. aureofaciens), 에스. 아우레우스(S. aureus), 에스. 푼기시디쿠스(S. fungicidicus), 에스. 그리세우스(S. griseus), 및 에스. 리비단스(S. lividans))이다. 일부 실시양태에서, 박테리아 숙주 세포는 자이모모나스 종(예를 들어, 지. 모빌리스(Z. mobilis), 및 지. 리폴리티카(Z. lipolytica))이다.

본 발명에서 유용한 많은 원핵 및 진핵 균주는 아메리칸 타입 컬쳐 컬렉션(ATCC), 도이체 잠룽 폰 미크로오르가니스멘 운트 젤쿨투렌 게엠베하(DSM: Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH), 센트랄뷰로 포르 쉼멜쿨투레스(CBS: Centraalbureau Voor Schimmelcultures) 및 애그리컬쳐럴 리써치 서비스 패턴트 컬쳐 컬렉션(Agricultural Research Service Patent Culture Collection), 노던 리져널 리써치 센터(NRRL: Northern Regional Research Center)와 같은 다수의 배양물 기탁 기관으로부터 공개적으로 쉽게 이용할 수 있다.

일부 실시양태에서, 숙주 세포는 단백질 분비, 단백질 안정성 및/또는 단백질의 발현 및/또는 분비에 바람직한 다른 특성을 개선하는 특징을 갖도록 유전적으로 변형된다. 유전자 변형은 유전공학 기술 및/또는 고전적인 미생물 기술(예를 들어, 화학적 또는 UV 돌연변이 유발 및 후속 선별)에 의해 달성될 수 있다. 실제로, 일부 실시양태에서, 재조합 변형 및 고전적인 선별 기술의 조합이 숙주 세포를 생산하기 위해 사용된다. 재조합 기술을 사용하여, 핵산 분자는 숙주 세포 내에서 및/또는 배양 배지에서 포스포펜토뮤타제 변이체(들)의 수율을 증가시키는 방식으로 도입, 결실, 억제 또는 변형될 수 있다. 예를 들어, Alp1 기능의 녹아웃(knockout)은 프로테아제가 결핍된 세포를 생성하고, pyr5 기능의 녹아웃은 피리미딘 결핍 표현형을 가진 세포를 생성한다. 하나의 유전공학 방법에서, 상동성 재조합은 코딩된 단백질의 발현을 억제하기 위해 생체 내에서 유전자를 특이적으로 표적화함으로써 표적화된 유전자 변형을 유도하기 위해 사용된다. 대안적 접근법에서, siRNA, 안티센스 및/또는 리보자임 기술이 유전자 발현 억제에 유용하다. 단백질을 코딩하는 유전자의 전부 또는 일부의 결실 및 유전자 생성물의 발현 또는 활성을 파괴하기 위한 부위-특이적 돌연변이 유발을 포함하지만 이로 제한되지 않는 세포에서 단백질의 발현을 감소시키는 다양한 방법이 관련 기술 분야에 공지되어 있다(예를 들어, 그 전체가 본원에 참고로 포함된 문헌 [Chaveroche et al., Nucl. Acids Res., 28:22 e97 [2000]; Cho et al., Molec. Plant Microbe Interact., 19:7-15 [2006]; Maruyama and Kitamoto, Biotechnol Lett., 30:1811-1817 [2008]; Takahashi et al., Mol. Gen. Genom., 272: 344-352 [2004]; 및 You et al., Arch. Microbiol.,191:615-622 [2009] 참조). 무작위 돌연변이 유발, 이어서 원하는 돌연변이에 대한 스크리닝도 유용하다(예를 들어, 둘 모두 참고로 포함된 문헌 [Combier et al., FEMS Microbiol. Lett., 220:141-8 [2003]; 및 Firon et al., Eukary. Cell 2:247-55 [2003]] 참조)).

벡터 또는 DNA 구축물을 숙주 세포에 도입하는 것은 인산칼슘 형질감염, DEAE-덱스트란 매개 형질감염, PEG 매개 형질전환, 전기천공 또는 관련 기술 분야에 공지된 다른 통상적인 기술을 포함하지만 이로 제한되지 않는, 관련 기술 분야에 공지된 임의의 적합한 방법을 사용하여 달성될 수 있다. 일부 실시양태에서, 에셔리키아 콜라이 발현 벡터 pCK100900i(본 명세서에 참조로 포함된 미국 특허 제9,714,437호 참조)가 유용하다.

일부 실시양태에서, 본 발명의 조작된 숙주 세포(즉, "재조합 숙주 세포")는 프로모터를 활성화하거나, 형질전환체를 선별하거나, 포스포펜토뮤타제 폴리뉴클레오타이드를 증폭하기 위해 적절하게 변형된 통상적인 영양 배지에서 배양된다. 배양 조건, 예를 들어 온도, pH 등은 발현을 위해 선택된 숙주 세포와 함께 이전에 사용된 것이며, 관련 기술 분야에서 통상의 기술을 가진 자에게 잘 알려져 있다. 언급한 바와 같이, 박테리아, 식물, 동물(특히 포유동물) 및 고세균 기원의 세포를 포함하는 많은 세포의 배양 및 생산에 대한 많은 표준 참고문헌 및 텍스트를 사용할 수 있다.

일부 실시양태에서, 본 발명의 변이체 포스포펜토뮤타제 폴리펩타이드를 발현하는 세포는 배치 또는 연속 발효 조건 하에서 성장된다. 고전적인 "배치 발효"는 폐쇄 시스템으로서, 배지의 조성은 발효 초기에 설정되고, 발효 동안 인공적으로 변경되지 않는다. 배치 시스템의 변형은 본 발명에서도 사용되는 "유가식 발효"이다. 이 변형에서, 기질은 발효가 진행됨에 따라 증분으로 추가된다. 유가식 시스템은 이화대사물 억제가 세포의 대사를 억제할 가능성이 있고 배지에 제한된 양의 기질이 존재하는 것이 바람직한 경우에 유용하다. 배치 및 유가식 발효는 일반적이며, 관련 기술 분야에 잘 알려져 있다. "연속 발효"는 개방 시스템으로서, 규정된 발효 배지가 생물반응기에 연속적으로 첨가되고 등량의 조건화된 배지가 처리를 위해 동시에 제거된다. 일반적으로 연속 발효는 배양액을 세포를 주로 로그 단계 성장에서 일정한 고밀도로 유지한다. 연속 발효 시스템은 항정 상태의 성장 조건을 유지하기 위해 노력한다. 연속 발효 공정을 위한 영양소 및 성장 인자를 조절하는 방법 및 생성물 형성 속도를 최대화하는 기술은 산업 미생물학 분야에서 잘 알려져 있다.

본 발명의 일부 실시양태에서, 무세포 전사/번역 시스템이 변이체 포스포펜토뮤타제(들)를 생산하기 위해 사용된다. 여러 시스템이 상업적으로 이용 가능하며, 그 방법은 관련 기술 분야에서 통상의 기술을 가진 자에게 잘 알려져 있다.

본 발명은 변이체 포스포펜토뮤타제 폴리펩타이드 또는 이의 생물학적 활성 단편을 제조하는 방법을 제공한다. 일부 실시양태에서, 상기 방법은 서열 번호 2, 4, 118, 266, 420, 562, 656, 790, 및/또는 846에 대한 적어도 약 70%(또는 적어도 약 75%, 적어도 약 80%, 적어도 약 85%, 적어도 약 90%, 적어도 약 95%, 적어도 약 96%, 적어도 약 97%, 적어도 약 98%, 또는 적어도 약 99%)의 서열 동일성을 포함하고 본원에서 제공되는 바와 같은 적어도 하나의 돌연변이를 포함하는 아미노산 서열을 코딩하는 폴리뉴클레오타이드로 형질전환된 숙주 세포를 제공하는 단계, 숙주 세포가 코딩되는 변이체 포스포펜토뮤타제 폴리펩타이드를 발현하는 조건 하에서 형질전환된 숙주 세포를 배양 배지에서 배양하는 단계; 및 선택적으로, 발현된 변이체 포스포펜토뮤타제 폴리펩타이드를 회수 또는 단리하고/하거나, 발현된 변이체 포스포펜토뮤타제 폴리펩타이드를 함유하는 배양 배지를 회수 또는 단리하는 단계를 포함한다. 일부 실시양태에서, 상기 방법은 선택적으로, 코딩되는 포스포펜토뮤타제 폴리펩타이드를 발현한 후에 형질전환된 숙주 세포를 용해시키고, 선택적으로, 발현된 변이체 포스포펜토뮤타제 폴리펩타이드를 세포 용해물로부터 회수 및/또는 단리하는 것을 추가로 제공한다. 본 발명은 또한 변이체 포스포펜토뮤타제 폴리펩타이드의 생산에 적합한 조건 하에서 변이체 포스포펜토뮤타제 폴리펩타이드로 형질전환된 숙주 세포를 배양하고, 변이체 포스포펜토뮤타제 폴리펩타이드를 회수하는 것을 포함하는, 변이체 포스포펜토뮤타제 폴리펩타이드의 제조 방법을 추가로 제공한다. 전형적으로, 포스포펜토뮤타제 폴리펩타이드의 회수 또는 단리는 본원에서 설명되는 것을 포함하여 관련 기술 분야에 잘 알려진 단백질 회수 기술을 사용하여 숙주 세포 배양 배지, 숙주 세포 또는 둘 모두로부터 이루어진다. 일부 실시양태에서, 숙주 세포는 원심분리에 의해 수거되고, 물리적 또는 화학적 수단에 의해 파괴되고, 생성된 조질 추출물은 추가의 정제를 위해 보유된다. 단백질 발현에 사용되는 미생물 세포는 동결-해동 사이클, 초음파 처리, 기계적 파괴 및/또는 세포 용해제의 사용을 포함하지만 이로 제한되지 않는 임의의 편리한 방법뿐만 아니라, 관련 기술 분야에서 통상의 기술을 가진 자에게 잘 알려진 많은 다른 적합한 방법에 의해 파괴될 수 있다.

숙주 세포에서 발현되는 조작된 포스포펜토뮤타제 효소는 특히 리소자임 처리, 초음파 처리, 여과, 염석, 초원심분리 및 크로마토그래피를 포함하는, 단백질 정제를 위해 관련 기술 분야에 공지된 임의의 하나 이상의 기술을 사용하여 세포 및/또는 배양 배지로부터 회수될 수 있다. 이. 콜라이와 같은 박테리아로부터 단백질을 용해하고 고효율로 추출하기에 적합한 용액은 상표명 CelLytic B™(Sigma-Aldrich)로 시판된다. 따라서, 일부 실시양태에서, 생성된 폴리펩타이드는 관련 기술 분야에 공지된 다수의 방법 중 임의의 것에 의해 회수/단리되고, 선택적으로 정제된다. 예를 들어, 일부 실시양태에서, 폴리펩타이드는 원심분리, 여과, 추출, 분무 건조, 증발, 크로마토그래피(예를 들어, 이온 교환, 친화도, 소수성 상호작용, 크로마토포커싱 및 크기 배제) 또는 침전을 포함하지만 이로 제한되지 않는 통상적인 절차에 의해 영양 배지로부터 단리된다. 일부 실시양태에서, 단백질 재폴딩 단계가 성숙 단백질의 구성을 완료할 때 필요에 따라 사용된다. 또한, 일부 실시양태에서, 고성능 액체 크로마토그래피(HPLC)가 최종 정제 단계에서 사용된다. 예를 들어, 일부 실시양태에서, 관련 기술 분야에 공지된 방법이 본 발명에서 유용하다(예를 들어, 둘 모두 본원에 참조로 포함된 문헌 [Parry et al., Biochem. J., 353:117 [2001]; 및 Hong et al., Appl. Microbiol. Biotechnol., 73:1331 [2007]] 참조). 실제로, 관련 기술 분야에 알려진 임의의 적합한 정제 방법이 본 발명에서 유용하다.

포스포펜토뮤타제 폴리펩타이드의 단리를 위한 크로마토그래피 기술은 역상 크로마토그래피 고성능 액체 크로마토그래피, 이온 교환 크로마토그래피, 겔 전기영동 및 친화도 크로마토그래피를 포함하지만 이로 제한되지 않는다. 특정 효소를 정제하기 위한 조건은 부분적으로는, 순 전하, 소수성, 친수성, 분자량, 분자 형태 등과 같은 인자에 따라 달라질 것이며, 이는 관련 기술 분야에서 통상의 기술을 가진 자에게 공지되어 있다.

일부 실시양태에서, 친화도 기술은 개선된 포스포펜토뮤타제 효소를 단리하는데 유용하다. 친화도 크로마토그래피 정제를 위해, 포스포펜토뮤타제 폴리펩타이드에 특이적으로 결합하는 임의의 항체가 사용될 수 있다. 항체 생산을 위해, 토끼, 마우스, 래트 등을 포함하지만 이로 제한되지 않는 다양한 숙주 동물이 포스포펜토뮤타제 주사에 의해 면역화될 수 있다. 포스포펜토뮤타제 폴리펩타이드는 측쇄 작용기 또는 측쇄 작용기에 부착된 링커에 의해 BSA와 같은 적합한 담체에 부착될 수 있다. 프로인트(Freund)(완전 및 불완전), 수산화알루미늄과 같은 미네랄 겔, 리솔레시틴과 같은 표면 활성 물질, 플루로닉 폴리올, 다가음이온, 펩타이드, 오일 에멀젼, 키홀 림펫 헤모시아닌, 디니트로페놀, 및 잠재적으로 유용한 인간 보조제, 예를 들어 BCG(바실러스 칼메테 구에린 (Bacillus Calmette Guerin)) 및 코리네박테리움 피르붐(Corynebacterium parvum)을 포함하지만 이로 제한되지 않는 숙주 종에 따라 면역 반응을 증가시키기 위해 다양한 보조제가 사용될 수 있다.

일부 실시양태에서, 포스포펜토뮤타제 변이체는 효소를 발현하는 세포의 형태로, 조질 추출물로서, 또는 단리되거나 정제된 제제로서 제조되고 사용된다. 일부 실시양태에서, 포스포펜토뮤타제 변이체는 동결건조물, 분말 형태(예를 들어, 아세톤 분말)로 제조되거나, 효소 용액으로 제조된다. 일부 실시양태에서, 포스포펜토뮤타제 변이체는 실질적으로 순수한 제제의 형태로 존재한다.

일부 실시양태에서, 포스포펜토뮤타제 폴리펩타이드는 임의의 적합한 고체 기재에 부착된다. 고체 기재는 고체상, 표면 및/또는 막을 포함하지만, 이로 제한되지 않는다. 고체 지지체는 폴리스티렌, 폴리에틸렌, 폴리프로필렌, 폴리플루오로에틸렌, 폴리에틸렌옥시 및 폴리아크릴아미드뿐만 아니라, 이들의 공중합체 및 그라프트를 포함하지만, 이로 제한되지 않는다. 고체 지지체는 또한 무기물, 예를 들어 유리, 실리카, 제어된 기공 유리(CPG), 역상 실리카 또는 금 또는 백금과 같은 금속일 수 있다. 기재의 배열은 비드, 구체, 입자, 과립, 겔, 막 또는 표면의 형태일 수 있다. 표면은 평면형, 실질적으로 평면형 또는 비평면형일 수 있다. 고체 지지체는 다공성이거나 비다공성일 수 있으며, 팽윤 또는 비팽윤 특성을 가질 수 있다. 고체 지지체는 웰, 침하부 또는 다른 컨테이너, 용기, 특징체(feature) 또는 구획(location)의 형태로 구성될 수 있다. 시약의 로봇에 의한 전달을 위해 또는 검출 방법 및/또는 기기에 의해 주소 지정 가능한 다양한 위치의 어레이에 복수의 지지체를 구성할 수 있다.

일부 실시양태에서, 포스포펜토뮤타제 변이체를 정제하기 위해 면역학적 방법이 사용된다. 한 접근법에서, 통상적인 방법을 사용하여 야생형 또는 변이체 포스포펜토뮤타제 폴리펩타이드에 대해 생성된 항체(예를 들어, 서열 번호 2, 4, 118, 266, 420, 562, 656, 790, 및/또는 846 중 어느 하나를 포함하는 폴리펩타이드, 및/또는 이의 변이체, 및/또는 이의 면역원성 단편에 대한 항체)를 비드에 고정시키고, 변이체 포스포펜토뮤타제가 결합되어 침전되는 조건 하에서 세포 배양 배지와 혼합한다. 관련 접근 방식에서, 면역 크로마토그래피가 유용하다.

일부 실시양태에서, 변이체 포스포펜토뮤타제는 비-효소 부분을 포함하는 융합 단백질로서 발현된다. 일부 실시양태에서, 변이체 포스포펜토뮤타제 서열은 정제 촉진 도메인에 융합된다. 본원에서 사용되는 바와 같이, 용어 "정제 촉진 도메인"은 그것이 융합되는 폴리펩타이드의 정제를 매개하는 도메인을 지칭한다. 적합한 정제 도메인은 금속 킬레이팅 펩타이드, 고정된 금속 상에서의 정제를 허용하는 히스티딘-트립토판 모듈, 글루타티온(예를 들어, GST)에 결합하는 서열, 헤마글루티닌(HA) 태그(인플루엔자 헤마글루티닌 단백질로부터 유래된 에피토프에 상응함; 예를 들어, 문헌 [Wilson et al., Cell 37:767 [1984]] 참조), 말토오스 결합 단백질 서열, FLAGS 연장/친화도 정제 시스템(예를 들어, Immunex Corp에서 입수 가능한 시스템)에서 사용되는 FLAG 에피토프 등을 포함하지만, 이로 제한되지 않는다. 본원에서 설명되는 조성물 및 방법에서 사용하기 위해 고려되는 하나의 발현 벡터는 엔테로키나제 절단 부위에 의해 분리된 폴리히스티딘 영역에 융합된 본 발명의 폴리펩타이드를 포함하는 융합 단백질의 발현을 제공한다. 히스티딘 잔기는 IMIAC(고정된 금속 이온 친화도 크로마토그래피; 문헌 [Porath el al., Prot. Exp. Purif., 3:263-281 [1992]] 참조)에서 정제를 촉진하는 반면, 엔테로키나제 절단 부위는 융합 단백질로부터 변이체 포스포펜토뮤타제 폴리펩타이드를 분리하기 위한 수단을 제공한다. pGEX 벡터(Promega)는 또한 글루타티온 S-트랜스퍼라제(GST)와의 융합 단백질로서 외래 폴리펩타이드를 발현하기 위해 사용될 수 있다. 일반적으로, 이러한 융합 단백질은 가용성이며, 리간드-아가로스 비드(예를 들어, GST-융합의 경우 글루타티온-아가로스)에 흡착한 다음, 유리 리간드의 존재 하에 용리함으로써 용해된 세포로부터 쉽게 정제될 수 있다.

따라서, 또 다른 측면에서, 본 발명은 조작된 효소 폴리펩타이드를 생산하는 방법을 제공하며, 상기 방법은 폴리펩타이드의 발현에 적합한 조건 하에서 조작된 효소 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 발현할 수 있는 숙주 세포를 배양하는 단계를 포함한다. 일부 실시양태에서, 상기 방법은 본원에서 설명되는 바와 같이 효소 폴리펩타이드를 단리 및/또는 정제하는 단계를 추가로 포함한다.

숙주 세포에 대한 적절한 배양 배지 및 성장 조건은 관련 기술 분야에 잘 알려져 있다. 효소 폴리펩타이드의 발현을 위한 폴리뉴클레오타이드를 세포 내로 도입하는 임의의 적합한 방법이 본 발명에서 유용할 것으로 고려된다. 적합한 기술은 전기천공, 바이올리스틱 입자 충격, 리포좀 매개 형질감염, 염화칼슘 형질감염 및 원형질체 융합을 포함하지만, 이로 제한되지 않는다.

본 발명의 다양한 특징 및 실시양태는 하기 대표적인 실시예에서 예시되고, 이것은 제한하는 것이 아니라 예시적인 것으로 의도된다.

실험

달성된 실험 및 결과를 포함하는 하기 실시예는 단지 예시 목적으로만 제공되는 것으로서, 본 발명을 제한하는 것으로 해석되지 않아야 한다. 실제로, 아래에서 설명되는 많은 시약 및 장비에 적합한 다양한 공급처가 존재한다. 본 발명은 임의의 시약 또는 장비 품목에 대한 임의의 특정 공급처로 제한되도록 의도되지 않는다.

아래에서 개시되는 실험에서, 다음 약어가 적용된다: M (몰); mM (밀리몰), uM 및 μM (마이크로몰); nM (나노몰); mol (몰); gm 및 g(그램); mg (밀리그램); ug 및 μg (마이크로그램); L 및 l (리터); ml 및 mL (밀리리터); cm (센티미터); mm (밀리미터); um 및 μm (마이크로미터); sec. (초); min(s) (분); h(s) 및 hr(s) (시간); U (단위); MW (분자량); rpm (분당 회전); psi 및 PSI (평방인치당 파운드); ℃(섭씨 온도); RT 및 rt(실온); CV(변동 계수); CAM 및 cam (클로람페니콜); PMBS (폴리믹신 B 설페이트); IPTG (이소프로필 β-D-1-티오갈락토피라노사이드); LB (용원성 브로쓰); TB (테리픽 브로쓰); SFP (진탕 플라스크 분말); CDS (코딩 서열); DNA (데옥시리보핵산); RNA (리보핵산); nt (뉴클레오타이드; 폴리뉴클레오타이드); aa(아미노산; 폴리펩타이드); 이. 콜라이 W3110(일반적으로 사용되는 실험용 이. 콜라이 균주, Coli Genetic Stock Center [CGSC] (미국 코네티컷주 뉴 헤이븐 소재)로부터 이용가능함); HTP (고처리량); HPLC (고압 액체 크로마토그래피); HPLC-UV (HPLC- 자외선 가시광선 검출기); 1H NMR (양성자 핵자기 공명 분광법); FIOPC (양성 대조군 대비 개선 배수); 시그마-알드리치 (Sigma-Aldrich, 미국 미주리주 세인트루이스 소재); 디프코 (Difco Laboratories, BD Diagnostic Systems, 미국 미시건주 디트로이트 소재); 마이크로플루이딕스 (Microfluidics, 미국 매사추세츠주 웨스트우드 소재); 라이프 테크놀로지스 (Life Technologies, Fisher Scientific의 자회사, 미국 매사추세츠주 월담 소재); 암레스코 (Amresco, LLC, 미국 오하이오주 솔론 소재); 카르보신트 (Carbosynth, Ltd., 영국 버크셔 소재); 바리안 (Varian Medical Systems, 미국 캘리포니아주 팔로 알토 소재); 애질런트 (Agilent Technologies, Inc., 미국 캘리포니아주 산타 클라라 소재); 인포르스 (Infors USA Inc., 미국 메릴랜드주 아나폴리스 정션 소재); 및 써모트론 (Thermotron, Inc., 미국 미시건주 홀란드 소재).

실시예 1

습식 세포 펠렛을 포함하는 HTP PPM의 제조

본 발명의 변이체를 생산하기 위해 사용된 PPM(서열 번호 2) 효소의 모 유전자는 이. 콜라이 게놈으로부터 수득하고, pCK110900 벡터에 클로닝하였다. W3110 이. 콜라이 세포를 PPM 코딩 유전자를 포함하는 각각의 플라스미드로 형질전환하고, 1% 글루코스 및 30 ㎍/ml 클로람페니콜(CAM)을 포함하는 LB 한천 플레이트에 플레이팅하고, 37℃에서 밤새 성장시켰다. 모노클로날 콜로니를 골라서 1% 글루코스 및 30 ㎍/mL 클로람페니콜을 함유하는 180 ㎕ LB에 접종하고, 96웰의 얕은 웰 미량역가 플레이트의 웰에 위치시켰다. 플레이트를 O₂ 투과성 밀봉재로 밀봉하고, 배양물을 30℃, 200 rpm 및 85% 습도에서 밤새 성장시켰다. 그런 다음, 10 ㎕의 각각의 세포 배양물을 390 ㎕ TB 및 30 ㎍/mL CAM을 포함하는 96웰의 깊은 웰 플레이트의 웰로 옮겼다. 깊은 웰 플레이트를 O₂ 투과성 밀봉재로 밀봉하고, OD₆₀₀ 0.6-0.8에 도달할 때까지 30℃, 250 rpm 및 85% 습도에서 밤새 인큐베이팅하였다. 그런 다음, 이소프로필 티오글리코사이드(IPTG)를 1 mM의 최종 농도로 첨가하여 세포 배양을 유도하고, 250 rpm으로 진탕하면서 30℃에서 밤새 인큐베이팅하였다. 이어서, 세포를 4,000 rpm에서 원심분리를 사용하여 세포를 펠렛화하였다. 상청액을 버리고, 펠렛을 용해 전에 -80℃에서 냉동시켰다.

실시예 2

HTP PPM 함유 세포 용해물의 제조

실시예 1에 기재된 바와 같이 제조된 동결 펠렛을 100 mM 트리에탄올아민 완충제, pH 7.5, 1 mg/mL 리소자임, 0.5 mg/mL PMBS 및 0.3-5 mM MnCl₂를 함유하는 400㎕ 용해 완충제로 용해시켰다. 용해 혼합물을 실온에서 2시간 동안 진탕하였다. 그런 다음, 플레이트를 4000 rpm 및 4℃에서 15분 동안 원심분리하였다. 이어서, 상청액은 활성 수준을 결정하기 위해 정화된 용해물로서 생촉매 반응에 사용되었다.

실시예 3

진탕 플라스크(SF) 배양물로부터 동결건조된 용해물의 제조

1% 글루코스 및 30 ㎍/ml CAM이 있는 LB 한천 플레이트로부터 원하는 유전자를 포함하고 37℃에서 밤새 인큐베이팅된 단일 콜로니를 1% 글루코스 및 30 ㎍/ml CAM이 있는 6 ml의 LB로 옮겼다. 배양물을 30℃, 250 rpm에서 18시간 동안 성장시키고, 30 ㎍/ml CAM을 포함하는 250 ml의 TB에 약 1:50으로 약 0.05의 최종 OD₆₀₀까지 계대배양하였다. 30℃, 250 rpm에서 약 195분 동안 0.6-0.8의 OD₆₀₀까지 계대배양물을 성장시키고, 1 mM IPTG로 유도하였다. 그런 다음, 계대배양물을 30℃, 250 rpm에서 20시간 동안 성장시켰다. 계대 배양물은 4000 rpm에서 20분 동안 원심분리하였다. 상청액을 버리고, 펠렛을 5 mM MnCl₂와 함께 35 ml의 25 mM 트리에탄올아민 완충제(pH 7.5)에 재현탁시켰다. 세포를 18,000 psi에서 마이크로플루이다이저(Microfluidizer)® 프로세서 시스템(Microfluidics)을 사용하여 용해시켰다. 용해물을 펠렛화하고(10,000 rpm x 60분), 상청액을 동결하고, 동결 건조하여 진탕 플레이크(SF) 효소 분말을 생성하였다.

실시예 4

화합물 4의 생산을 위한 개선된 포스포펜토뮤타제 변이체

서열 번호 2가 모 효소로서 선택되었다. 잘 확립된 기술(예를 들어, 포화 돌연변이 유발 및 이전에 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자의 라이브러리를 생성하였다. 각각의 유전자에 의해 코딩된 폴리펩타이드는 실시예 1에 기술된 바와 같이 HTP에서 생산되었고, 정화된 용해물은 실시예 2에 기술된 바와 같이 생성되었다.

각각의 효소에 대해, 정화된 세포 용해물을 0.3 mM MnCl₂를 함유하는, pH 7.5의 50 mM 트리에탄올아민(TEoA)에 50배 희석하였다. 각각 100 ㎕의 반응은 pH 7.5에서 50%(v/v) 희석된 용해물, 5 mM 화합물 3, 50 mM TEoA 완충제 및 0.15 mM MnCl₂를 사용하여 96웰 얕은 웰 미량역가 플레이트에서 수행하였다. 플레이트를 열 밀봉하고, 30℃에서 인큐베이팅하고, 인포르스 써모트론(Infors Thermotron)^® 진탕기에서 500 rpm으로 밤새 교반하였다. 플레이트를 제거하고, pH 8.0에서 1 mM EDTA를 함유하는 3 부피의 물로 희석하여 급냉시켰다.

서열 번호 2와 비교한 활성은 서열 번호 2의 것과 비교하여 변이체의 전환 비율당 형성된 생성물의 전환 비율로서 계산되었다. 전환 비율은 생성물 피크의 면적을 LCMS 분석에 의해 결정된 생성물 피크 및 기질의 면적의 총합으로 나눔으로써 정량하였다.

실시예 5

이 실험을 위한 모 효소로서 서열 번호 4를 선택하였다. 잘 확립된 기술(예를 들어, 포화 돌연변이 유발 및 이전에 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자의 라이브러리를 생성하였다. 각각의 유전자에 의해 코딩된 폴리펩타이드는 실시예 1에 기술된 바와 같이 HTP에서 생산되었고, 정화된 용해물은 용해 완충제에서 MnCl₂ 농도가 10 mM로 증가된 것을 제외하고는 실시예 2에 기술된 바와 같이 생성되었다.

각각의 효소에 대해, 정화된 세포 용해물을 10 mM MnCl₂를 함유하는, pH 7.5의 50 mM TEoA에 50배 희석하였다. 각각 100 ㎕의 반응은 pH 7.5에서 50%(v/v) 희석된 용해물, 20 mM 화합물 3, 50 mM TEoA 완충제 및 5.0 mM MnCl₂를 사용하여 96웰 얕은 웰 미량역가 플레이트에서 수행하였다. 플레이트를 열 밀봉하고, 45℃에서 인큐베이팅하고, 인포르스 써모트론^® 진탕기에서 500 rpm으로 밤새 교반하였다. 플레이트를 제거하고, pH 8.0에서 3 mM EDTA를 함유하는 2 부피의 물로 희석한 다음, 이를 물 내의 3 mM ETDA로 추가로 10x 희석하여 급냉시켰다.

서열 번호 4와 비교한 활성은 서열 번호 4의 전환 비율과 비교하여 변이체 효소에 의해 형성된 생성물의 전환 비율로서 계산되었다. 전환 비율은 생성물 피크의 면적을 LCMS 분석에 의해 결정된 생성물 피크 및 기질의 면적의 총합으로 나눔으로써 정량하였다.

실시예 6

이 실험을 위해, 모 효소로서 서열 번호 118을 선택하였다. 잘 확립된 기술(예를 들어, 포화 돌연변이 유발 및 이전에 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자의 라이브러리를 생성하였다. 각각의 유전자에 의해 코딩된 폴리펩타이드는 실시예 1에 기술된 바와 같이 HTP에서 생산되었고, 정화된 용해물은 실시예 2에 기술된 바와 같이 생성되었다.

화합물 3은 45℃에서 18시간 동안 750 rpm으로 교반되는 둥근 바닥 플라스크에서 100 mM 트리에탄올아민 완충제(pH 7.5) 내의 100 mL의 68 mM 화합물 1 및 145 mM 인산칼륨 및 100 mM 트리에탄올아민 완충제(pH 7.5) 내의 100 mL의 1 g/L PNP(서열 번호 1154)를 혼합함으로써 합성되었다. 반응 혼합물을 4000 rpm, 44℃에서 1.5시간 동안 원심분리하였다. 상청액을 동결시키고, 18시간 동안 동결 건조하였다.

각각의 효소에 대해, 정화된 세포 용해물을 pH 7.5의 50 mM TEoA에 50배 희석하였다. 각각 100 ㎕의 반응은 100 mM 트리에탄올 아민 완충제(pH 7.5) 내의 90 ㎕의 20 mM 화합물 3 및 10 ㎕의 희석된 정화된 용해물을 사용하여 96웰 얕은 웰 미량역가 플레이트에서 수행하였다. 플레이트를 밀봉하고, 40℃, 800 rpm에서 밤새 인큐베이팅하였다. 반응물을 물 내의 3부피의 3 mM ETDA로 급냉시키고, 탁상용 진탕기에서 15분 동안 진탕한 다음, 4000 rpm에서 4℃에서 10분 동안 원심분리하였다. 25 uL의 급냉시킨 샘플을 제거하여 샘플을 추가로 희석하고, LC-MS 분석을 위해 둥근 바닥 96웰 플레이트에서 7 uL의 3 mM EDTA로 옮겼다.

서열 번호 118과 비교한 활성은 특정 반응 조건 하에서 서열 번호 118에 의해 생산된 전환 비율과 비교하여 변이체 효소에 의해 형성된 생성물의 전환 비율로서 계산되었다. 전환 비율은 생성물 피크의 면적을 LCMS 분석에 의해 결정된 생성물 피크 및 기질의 면적의 총합으로 나눔으로써 정량하였다.

실시예 7

이 실험을 위해, 모 효소로서 서열 번호 266을 선택하였다. 잘 확립된 기술(예를 들어, 포화 돌연변이 유발 및 이전에 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자의 라이브러리를 생성하였다. 각각의 유전자에 의해 코딩된 폴리펩타이드는 실시예 1에 기술된 바와 같이 HTP에서 생산되었고, 정화된 용해물은 실시예 2에 기술된 바와 같이 생성되었다.

각각의 효소에 대해, 정화된 세포 용해물을 pH 7.5의 50 mM TEoA에 50배 희석하였다. 각각 100 ㎕의 반응은 100 mM 트리에탄올 아민 완충제(pH 7.5) 내의 90 ㎕의 20 mM 화합물 3 및 10 ㎕의 희석된 정화된 용해물을 사용하여 96웰 얕은 웰 미량역가 플레이트에서 수행하였다. 플레이트를 밀봉하고, 40℃, 800 rpm에서 18시간 동안 인큐베이팅하였다. 반응물을 3부피의 3 mM ETDA로 급냉시키고, 탁상용 진탕기에서 15분 동안 진탕한 다음, 4000 rpm에서 4℃에서 10분 동안 원심분리하였다.

급냉시킨 역반응물 50 ㎕를 1-포스포데옥시리보스(화합물 3)를 제거하기 위해 100 mM 플루오로아데닌(화합물 2), 0.5 g/L의 PNP(서열 번호 1154), 0.25 g/L의 수크로스 포스포릴라제(서열 번호 1158) 및 100 mM 트리에탄올 아민 완충제(pH = 7.5)를 포함하는 50 ㎕의 기질 용액으로 처리하였다. 반응물을 800 rpm으로 진탕하면서 45℃에서 4시간 동안 인큐베이팅하였다. 플레이트를 4℃ 및 4000 rpm에서 15분 동안 원심분리한 후, 50 ㎕의 상청액을 제거하고, LCMS 분석을 위해 50 ㎕의 3 mM EDTA로 희석하였다. 서열 번호 266과 비교한 활성은 LCMS 분석에 의해 결정된, 특정 반응 조건 하에서 변이체 효소에 의해 형성된 생성물의 피크 면적 대 LCMS 분석에 의해 결정된, 서열 번호 266에 의해 생산된 생성물의 피크 면적의 비로서 계산되었다.

실시예 8

화합물 1의 생산을 위한 개선된 포스포펜토뮤타제 변이체

각각 100 ㎕의 반응은 반응식 III에 제시된 바와 같이 96웰 얕은 웰 미량역가 플레이트에서 수행하였다. 구체적으로, 25 g/L 화합물 4, 18 g/L 화합물 2; 1.5 g/L PNP(서열 번호 1154), 5 mM MnCl₂, 196 mM 수크로스, 0.25 g/L 수크로스 포스포릴라제(서열 번호 1158), 50 mM 트리에탄올 아민 완충제(pH 7.5) 및 20 ㎕ 조질 PPM 용해물. 플레이트를 열 밀봉하고, 40℃에서 멀티트론(Multitron)(Infors)에서 800 rpm으로 약 18시간 동안 인큐베이팅하였다. 샘플을 3 부피의 1 M KOH:DMSO(50:50)에서 급냉시키고, 5분 동안 진탕한 후, HPLC 분석을 위해 10x(180 ㎕의 75% 0.1 M 트리에탄올아민(pH 7.5) 및 25% MeCN 내로의 20 ㎕) 희석하였다.

서열 번호 266과 비교한 활성은 특정 반응 조건 하에서 서열 번호 266의 전환 비율과 비교하여 변이체 효소에 의해 형성된 생성물의 전환 비율로서 계산되었다. 전환 비율은 생성물 피크(화합물 1)의 면적을 LCMS 분석에 의해 결정된 기질(화합물 2), 생성물(화합물 1) 및 불순물/부산물 피크의 면적의 총합으로 나눔으로써 정량하였다.

실시예 9

후속 진화 라운드를 위한 모 효소로서 서열 번호 420을 선택하였다. 잘 확립된 기술(예를 들어, 포화 돌연변이 유발 및 이전에 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자의 라이브러리를 생성하였다. 각각의 유전자에 의해 코딩된 폴리펩타이드는 실시예 1에 기술된 바와 같이 HTP에서 생산되었고, 정화된 용해물은 실시예 2에 기술된 바와 같이 생성되었다. 반응은 실시예 8에서 설명된 바와 같이 수행하였다.

실시예 10

후속 진화 라운드를 위한 모 효소로서 서열 번호 420을 선택하였다. 잘 확립된 기술(예를 들어, 포화 돌연변이 유발 및 이전에 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자의 라이브러리를 생성하였다. 각각의 유전자에 의해 코딩된 폴리펩타이드는 실시예 1에 기술된 바와 같이 HTP에서 생산되었다. 정화된 용해물은 실시예 2에 기술된 바와 같이 생성되었다. 반응은 실시예 8에서 설명된 바와 같이 수행하였다.

실시예 11

분석 방법

이 실시예는 상기 실시예에서 제공된 데이터를 수집하기 위해 사용되는 방법을 제공한다. 실시예 8-10에 기재된 바와 같이 수득된 데이터는 표 11.1의 분석 방법을 사용하여 수집되었다. 본 실시예에서 제공된 방법은 본 발명을 사용하여 생성된 변이체를 분석하는 데 사용된다. 그러나, 다른 적절한 방법이 관련 기술 분야에서 통상의 기술을 가진 자에게 공지되어 있으므로, 본 발명이 본원에서 설명되는 방법으로 제한되는 것으로 의도되지 않는다.

1- 및 5-포스포데옥시리보스 알킨의 분석 검출을 위해 실시예 4-6에 기술된 데이터는 표 11.2의 분석 방법을 사용하여 수집되었다

역반응에서 5-포스포데옥시리보스의 분석 검출을 위해 실시예 7에 기재된 바와 같이 수득된 데이터는 표 11.3의 분석 방법을 사용하여 수집되었다.

실시예 12

화합물 1의 생산을 위한 서열 번호 562의 개선된 포스포펜토뮤타제 변이체

서열 번호 562의 포스포펜토뮤타제 활성을 갖는 폴리펩타이드를 코딩하는 조작된 폴리뉴클레오타이드(서열 번호 561)를 사용하여 표 12.1 및 12.2의 조작된 폴리펩타이드를 생성하였다. 이들 폴리펩타이드는 출발 폴리펩타이드와 비교하여 원하는 조건 하에서 개선된 포스포펜토뮤타제 활성(예를 들어, 반응식 IV에 제시된 바와 같이 SP 및 조작된 DERA 및 PNP 효소의 존재 하에 화합물 1의 생산을 통해 측정된 바와 같이 화합물 3을 생산하는 능력)을 나타냈다.

반응식 IV

짝수 번호의 서열 식별자의 아미노산 서열을 갖는 조작된 폴리펩타이드는 아래에서 설명되는 바와 같이 서열 번호 562의 "백본" 아미노산 서열로부터 생성되었다. 유도 진화는 서열 번호 561에 제시된 폴리뉴클레오타이드로 시작되었다. 조작된 폴리펩타이드의 라이브러리는 다양한 잘 알려진 기술(예를 들어, 포화 돌연변이 유발, 이전에 확인된 유익한 아미노산 차이의 재조합)을 사용하여 생성되었으며, HTP 분석 및 폴리펩타이드 포스포펜토뮤타제 활성을 측정하는 분석 방법을 사용하여 스크리닝되었다. 이 경우, 활성은 표 12.3의 분석 방법을 사용하여 반응식 IV에 제시된 바와 같이 수크로스 포스포릴라제(SP) 및 조작된 데옥시리보스-포스페이트 알돌라제(DERA) 및 퓨린 뉴클레오사이드 포스포릴라제(PNP) 효소의 존재 하에 화합물 1의 생산을 통해 측정되었다. 본원에서 제공되는 방법은 본 발명을 사용하여 생성된 변이체를 분석하는 데 사용된다. 그러나, 다른 적합한 방법이 본 발명에서 유용하기 때문에, 본원에서 설명되는 방법이 본원에서 제공되고/되거나 본원에서 제공되는 방법을 사용하여 생성된 변이체의 분석에 적용 가능한 유일한 방법이라고 의도되지 않는다.

고처리량 용해물은 다음과 같이 제조되었다. 클론 PPM 변이체로부터의 냉동 펠렛을 실시예 1에 기술된 바와 같이 제조하고, 100 mM 트리에탄올아민 완충제(pH 7.5), 1 mg/mL 리소자임 및 0.5 mg/mL PMBS를 함유하는 400 ㎕ 용해 완충제로 용해시켰다. 용해 혼합물을 실온에서 2시간 동안 진탕하였다. 그런 다음, 플레이트를 4000 rpm 및 4℃에서 15분 동안 원심분리하였다.

진탕 플라스크 분말(진탕 플라스크 배양물로부터 동결 건조된 용해물)은 다음과 같이 제조되었다. 원하는 변이체의 세포 배양물을 1% 글루코스 및 30 ㎍/ml CAM을 포함하는 LB 한천 플레이트에 플레이팅하고, 37℃에서 밤새 성장시켰다. 각각의 배양물의 단일 콜로니를 1% 글루코스 및 30 ㎍/ml CAM을 함유하는 6 ml의 LB로 옮겼다. 배양물을 30℃, 250 rpm에서 18시간 동안 성장시키고, 30 ㎍/ml CAM을 포함하는 250 ml의 TB에 약 1:50으로 약 0.05의 최종 OD₆₀₀까지 계대배양하였다. 30℃, 250 rpm에서 약 195분 동안 0.6-0.8의 OD₆₀₀까지 계대배양물을 성장시키고, 1 mM IPTG로 유도하였다. 그런 다음, 배양물을 30℃, 250 rpm에서 20시간 동안 성장시켰다. 배양물은 4000 rpm에서 10분 동안 원심분리하였다. 상청액을 버리고, 펠렛을 30 ml의 25 mM 트리에탄올아민(pH 7.5)에 재현탁하고, 18,000 psi에서 마이크로플루이다이저® 프로세서 시스템(Microfluidics)을 사용하여 용해시켰다. 용해물을 펠렛화하고(60분 동안 10,000 rpm), 상청액을 동결하고, 동결 건조하였다.

반응은 총 부피가 100 ㎕인 2 mL의 깊은 웰 플레이트에 96웰 형식의 DERA/PPM/PNP/SP 효소를 포함하는 직렬 4-효소 캐스케이드 설정에서 수행되었다. 반응물은 진탕 플라스크 분말로서 DERA, PNP 및 SP(0.5 중량% DERA 서열 번호 1162 및 0.5 중량% PNP 서열 번호 1156, 4 중량% 야생형 SP- 서열 번호 1158), 26 g/L 또는 124 mM의 순수 거울상(enantiopure) (R)-2-에티닐-글리세르알데하이드 기질, 99 mM F-아데닌(0.8 eq.), 186 mM 아세트알데하이드(이소프로판올 중 40 중량%, 1.5 eq.), 372 mM 수크로스(3.0 eq.), 5 mM MnCl₂ 및 50 mM TEoA(pH 7.5)를 포함하였다. 반응은 다음과 같이 설정되었다: (i) PPM을 제외한 모든 반응 성분을 단일 용액에서 미리 혼합하고, 이어서 90 ㎕의 상기 용액을 96웰 플레이트의 각각의 웰에 분취하고, 이어서, (ii) 10 ㎕의 PPM 용해물을 웰에 첨가하여 반응을 개시시켰다. 반응 플레이트를 열 밀봉하고, 18-20시간 동안 600 rpm에서 진탕하면서 35℃에서 인큐베이팅하였다.

반응물을 1 M KOH 및 DMSO의 300 ㎕의 1:1 혼합물로 급냉시켰다. 급냉된 반응물을 탁상용 진탕기에서 10분 동안 진탕한 다음, 4000 rpm에서 4℃에서 5분 동안 원심분리하여 임의의 침전물을 펠렛화하였다. 10 마이크로리터의 상청액을 0.1 M TEoA(pH 7.5) 완충제 내의 190 ㎕의 25% MeCN으로 미리 채워진 96웰 둥근 바닥 플레이트로 옮겼다. 샘플을 써모 U3000 UPLC 시스템에 주입하고, 75:25의 물:실시예 12-3에 설명된 바와 같이 0.1% TFA가 보충된 아세토니트릴을 포함하는 이동상으로 등용매 방식으로 아틀란티스 T3 C18, 3 ㎛, 2.1 x 100 mm 컬럼을 사용하여 분리하였다. 서열 번호 562와 비교한 활성은 특정 반응 조건 하에서 서열 번호 562에 의해 형성된 화합물 1의 피크 면적과 비교한, 변이체 효소에 의해 형성된 화합물 1의 피크 면적으로서 계산되었다.

실시예 13

화합물 1의 생산을 위한 서열 번호 656의 개선된 포스포펜토뮤타제 변이체

서열 번호 656의 포스포펜토뮤타제 활성을 갖는 폴리펩타이드를 코딩하는 조작된 폴리뉴클레오타이드(서열 번호 655)를 사용하여 표 13.1의 조작된 폴리펩타이드를 생성하였다. 이들 폴리펩타이드는 출발 폴리펩타이드와 비교하여 원하는 조건 하에서 개선된 포스포펜토뮤타제 활성(예를 들어, 반응식 IV에 제시된 바와 같이 조작된 DERA, PNP, 및 SP 효소의 존재 하에 화합물 1의 생산을 통해 측정된 바와 같이 화합물 3을 생산하는 능력)을 나타냈다.

짝수 번호의 서열 식별자의 아미노산 서열을 갖는 조작된 폴리펩타이드는 아래에서 설명되는 바와 같이 서열 번호 656의 "백본" 아미노산 서열로부터 생성되었다. 유도 진화는 서열 번호 655에 제시된 폴리뉴클레오타이드로 시작되었다. 조작된 폴리펩타이드의 라이브러리는 다양한 잘 알려진 기술(예를 들어, 포화 돌연변이 유발, 이전에 확인된 유익한 아미노산 차이의 재조합)을 사용하여 생성되었으며, HTP 분석 및 폴리펩타이드 포스포펜토뮤타제 활성을 측정하는 분석 방법을 사용하여 스크리닝되었다. 이 경우, 활성은 표 12.3의 분석 방법을 사용하여 반응식 IV에 제시된 바와 같이 조작된 수크로스 포스포릴라제(SP), 데옥시리보스-포스페이트 알돌라제(DERA) 및 퓨린 뉴클레오사이드 포스포릴라제(PNP) 효소의 존재 하에 화합물 1의 생산을 통해 측정되었다. 본원에서 제공되는 방법은 본 발명을 사용하여 생성된 변이체를 분석하는 데 사용된다. 그러나, 다른 적합한 방법이 본 발명에서 유용하기 때문에, 본원에서 설명되는 방법이 본원에서 제공되고/되거나 본원에서 제공되는 방법을 사용하여 생성된 변이체의 분석에 적용 가능한 유일한 방법이라고 의도되지 않는다.

진탕 플라스크 분말(진탕 플라스크 배양물로부터 동결 건조된 용해물)은 다음과 같이 제조되었다. 원하는 변이체의 세포 배양물을 1% 글루코스 및 30 ㎍/ml CAM을 포함하는 LB 한천 플레이트에 플레이팅하고, 37℃에서 밤새 성장시켰다. 각각의 배양물의 단일 콜로니를 1% 글루코스 및 30 ㎍/ml CAM을 함유하는 6 ml의 LB로 옮겼다. 배양물을 30℃, 250 rpm에서 18시간 동안 성장시키고, 30 ㎍/ml CAM을 포함하는 250 ml의 TB에 약 1:50으로 0.05의 최종 OD₆₀₀까지 계대배양하였다. 30℃, 250 rpm에서 약 195분 동안 0.6-0.8의 OD₆₀₀까지 계대배양물을 성장시키고, 1 mM IPTG로 유도하였다. 그런 다음, 배양물을 30℃, 250 rpm에서 20시간 동안 성장시켰다. 배양물은 4000 rpm에서 10분 동안 원심분리하였다. 상청액을 버리고, 펠렛을 30 ml의 25 mM 트리에탄올아민(pH 7.5)에 재현탁하고, 18,000 psi에서 마이크로플루이다이저® 프로세서 시스템(Microfluidics)을 사용하여 용해시켰다. 용해물을 펠렛화하고(10,000 rpm x 60분), 상청액을 동결하고, 동결 건조하였다.

반응은 총 부피가 100 ㎕인 2 mL의 깊은 웰 플레이트에 96웰 형식의 DERA/PPM/PNP/SP 효소를 포함하는 직렬 4-효소 캐스케이드 설정에서 수행되었다. 반응물은 진탕 플라스크 분말로서 DERA, PNP 및 SP(0.5 중량% DERA 서열 번호 1162 및 0.5 중량% PNP 서열 번호 1156, 0.5 중량% SP 서열 번호 1160), 26 g/L 또는 124 mM의 순수 거울상 (R)-2-에티닐-글리세르알데하이드 기질, 99 mM F-아데닌(0.8 eq.), 186 mM 아세트알데하이드(이소프로판올 중 40 중량%, 1.5 eq.), 372 mM 수크로스(3.0 eq.), 5 mM MnCl₂ 및 50 mM TEoA(pH 7.5)를 포함하였다. 반응은 다음과 같이 설정되었다: (i) PPM을 제외한 모든 반응 성분을 단일 용액에서 미리 혼합하고, 이어서 90 ㎕의 상기 용액을 96웰 플레이트의 각각의 웰에 분취하고, 이어서, (ii) 10 ㎕의 PPM 용해물을 웰에 첨가하여 반응을 개시시켰다. 반응 플레이트를 열 밀봉하고, 18-20시간 동안 600 rpm에서 진탕하면서 35℃에서 인큐베이팅하였다.

반응물을 1 M KOH 및 DMSO의 300 ㎕의 1:1 혼합물로 급냉시켰다. 급냉된 반응물을 탁상용 진탕기에서 10분 동안 진탕한 다음, 4000 rpm에서 4℃에서 5분 동안 원심분리하여 임의의 침전물을 펠렛화하였다. 이어서, 10 마이크로리터의 상청액을 0.1 M TEoA(pH 7.5) 완충제 내의 190 ㎕의 25% MeCN으로 미리 채워진 96웰 둥근 바닥 플레이트로 옮겼다. 샘플을 써모 U3000 UPLC 시스템에 주입하고, 75:25의 물:실시예 12-3에 설명된 바와 같이 0.1% TFA가 보충된 아세토니트릴을 포함하는 이동상으로 등용매 방식으로 아틀란티스 T3 C18, 3 ㎛, 2.1 x 100 mm 컬럼을 사용하여 분리하였다. 서열 번호 656과 비교한 활성은 특정 반응 조건 하에서 서열 번호 656에 의해 형성된 화합물 1의 피크 면적과 비교한, 변이체 효소에 의해 형성된 화합물 1의 피크 면적으로서 계산되었다.

실시예 14

화합물 1의 생산을 위한 서열 번호 790의 개선된 포스포펜토뮤타제 변이체

서열 번호 790의 포스포펜토뮤타제 활성을 갖는 폴리펩타이드를 코딩하는 조작된 폴리뉴클레오타이드(서열 번호 789)를 사용하여 표 14.1의 조작된 폴리펩타이드를 생성하였다. 이들 폴리펩타이드는 출발 폴리펩타이드와 비교하여 원하는 조건 하에서 개선된 포스포펜토뮤타제 활성(예를 들어, 반응식 IV에 제시된 바와 같이 조작된 DERA, PNP, 및 SP 효소의 존재 하에 화합물 1의 생산을 통해 측정된 바와 같이 화합물 3을 생산하는 능력)을 나타냈다.

짝수 번호의 서열 식별자의 아미노산 서열을 갖는 조작된 폴리펩타이드는 아래에서 설명되는 바와 같이 서열 번호 790의 "백본" 아미노산 서열로부터 생성되었다. 유도 진화는 서열 번호 790에 제시된 폴리뉴클레오타이드로 시작되었다. 조작된 폴리펩타이드의 라이브러리는 다양한 잘 알려진 기술(예를 들어, 포화 돌연변이 유발, 이전에 확인된 유익한 아미노산 차이의 재조합)을 사용하여 생성되었으며, HTP 분석 및 폴리펩타이드 포스포펜토뮤타제 활성을 측정하는 분석 방법을 사용하여 스크리닝되었다. 이 경우, 활성은 표 12.3의 분석 방법을 사용하여 반응식 IV에 제시된 바와 같이 조작된 수크로스 포스포릴라제(SP), 데옥시리보스-포스페이트 알돌라제(DERA) 및 퓨린 뉴클레오사이드 포스포릴라제(PNP) 효소의 존재 하에 화합물 1의 생산을 통해 측정되었다. 본원에서 제공되는 방법은 본 발명을 사용하여 생성된 변이체를 분석하는 데 사용된다. 그러나, 다른 적합한 방법이 본 발명에서 유용하기 때문에, 본원에서 설명되는 방법이 본원에서 제공되고/되거나 본원에서 제공되는 방법을 사용하여 생성된 변이체의 분석에 적용 가능한 유일한 방법이라고 의도되지 않는다.

반응은 총 부피가 100 ㎕인 2 mL의 깊은 웰 플레이트에 96웰 형식의 DERA/PPM/PNP/SP 효소를 포함하는 직렬 4-효소 캐스케이드 설정에서 수행되었다. 반응물은 진탕 플라스크 분말로서 DERA, PNP 및 SP(0.5 중량% DERA 서열 번호 1164, 0.5 중량% PNP 서열 번호 1156, 0.5 중량% SP 서열 번호 1160), 26 g/L 또는 124 mM의 순수 거울상 (R)-2-에티닐-글리세르알데하이드 기질, 99 mM F-아데닌(0.8 eq.), 186 mM 아세트알데하이드(이소프로판올 중 40 중량%, 1.5 eq.), 372 mM 수크로스(3.0 eq.), 5 mM MnCl₂ 및 50 mM TEoA(pH 7.5)를 포함하였다. 반응은 다음과 같이 설정되었다: (i) PPM을 제외한 모든 반응 성분을 단일 용액에서 미리 혼합하고, 이어서 90 ㎕의 상기 용액을 96웰 플레이트의 각각의 웰에 분취하고, 이어서, (ii) 10 ㎕의 PPM 용해물을 웰에 첨가하여 반응을 개시시켰다. 반응 플레이트를 열 밀봉하고, 18-20시간 동안 600 rpm에서 진탕하면서 35℃에서 인큐베이팅하였다.

반응물을 1 M KOH 및 DMSO의 300 ㎕의 1:1 혼합물로 급냉시켰다. 급냉된 반응물을 탁상용 진탕기에서 10분 동안 진탕한 다음, 4000 rpm에서 4℃에서 5분 동안 원심분리하여 임의의 침전물을 펠렛화하였다. 이어서, 10 마이크로리터의 상청액을 0.1 M TEoA(pH 7.5) 완충제 내의 190 ㎕의 25% MeCN으로 미리 채워진 96웰 둥근 바닥 플레이트로 옮겼다. 샘플을 써모 U3000 UPLC 시스템에 주입하고, 75:25의 물:실시예 12-3에 설명된 바와 같이 0.1% TFA가 보충된 아세토니트릴을 포함하는 이동상으로 등용매 방식으로 아틀란티스 T3 C18, 3 ㎛, 2.1 x 100 mm 컬럼을 사용하여 분리하였다. 서열 번호 790과 비교한 활성은 특정 반응 조건 하에서 서열 번호 790에 의해 형성된 화합물 1의 피크 면적과 비교한, 변이체 효소에 의해 형성된 화합물 1의 피크 면적으로서 계산되었다.

실시예 15

화합물 1의 생산을 위한 서열 번호 846의 개선된 포스포펜토뮤타제 변이체

서열 번호 846의 포스포펜토뮤타제 활성을 갖는 폴리펩타이드를 코딩하는 조작된 폴리뉴클레오타이드(서열 번호 845)를 사용하여 표 15.1의 조작된 폴리펩타이드를 생성하였다. 이들 폴리펩타이드는 출발 폴리펩타이드와 비교하여 원하는 조건 하에서 개선된 포스포펜토뮤타제 활성(예를 들어, 반응식 IV에 제시된 바와 같이 조작된 DERA, PNP, 및 SP 효소의 존재 하에 화합물 1의 생산을 통해 측정된 바와 같이 화합물 3을 생산하는 능력)을 나타냈다.

짝수 번호의 서열 식별자의 아미노산 서열을 갖는 조작된 폴리펩타이드는 아래에서 설명되는 바와 같이 서열 번호 846의 "백본" 아미노산 서열로부터 생성되었다. 유도 진화는 서열 번호 846에 제시된 폴리뉴클레오타이드로 시작되었다. 조작된 폴리펩타이드의 라이브러리는 다양한 잘 알려진 기술(예를 들어, 포화 돌연변이 유발, 이전에 확인된 유익한 아미노산 차이의 재조합)을 사용하여 생성되었으며, HTP 분석 및 폴리펩타이드 포스포펜토뮤타제 활성을 측정하는 분석 방법을 사용하여 스크리닝되었다. 이 경우, 활성은 표 12.3의 분석 방법을 사용하여 반응식 IV에 제시된 바와 같이 조작된 수크로스 포스포릴라제(SP), 데옥시리보스-포스페이트 알돌라제(DERA) 및 퓨린 뉴클레오사이드 포스포릴라제(PNP) 효소의 존재 하에 화합물 1의 생산을 통해 측정되었다. 본원에서 제공되는 방법은 본 발명을 사용하여 생성된 변이체를 분석하는 데 사용된다. 그러나, 다른 적합한 방법이 본 발명에서 유용하기 때문에, 본원에서 설명되는 방법이 본원에서 제공되고/되거나 본원에서 제공되는 방법을 사용하여 생성된 변이체의 분석에 적용 가능한 유일한 방법이라고 의도되지 않는다.

반응물을 1 M KOH 및 DMSO의 300 ㎕의 1:1 혼합물로 급냉시켰다. 급냉된 반응물을 탁상용 진탕기에서 10분 동안 진탕한 다음, 4000 rpm에서 4℃에서 5분 동안 원심분리하여 임의의 침전물을 펠렛화하였다. 이어서, 10 마이크로리터의 상청액을 0.1 M TEoA(pH 7.5) 완충제 내의 190 ㎕의 25% MeCN으로 미리 채워진 96웰 둥근 바닥 플레이트로 옮겼다. 샘플을 써모 U3000 UPLC 시스템에 주입하고, 75:25의 물:실시예 12-3에 설명된 바와 같이 0.1% TFA가 보충된 아세토니트릴을 포함하는 이동상으로 등용매 방식으로 아틀란티스 T3 C18, 3 ㎛, 2.1 x 100 mm 컬럼을 사용하여 분리하였다. 서열 번호 846과 비교한 활성은 특정 반응 조건 하에서 서열 번호 846에 의해 형성된 화합물 1의 피크 면적과 비교한, 변이체 효소에 의해 형성된 화합물 1의 피크 면적으로서 계산되었다.

본 출원에서 언급되는 모든 간행물, 특허, 특허 출원 및 기타 문서는 마치 각각의 개별 간행물, 특허, 특허 출원 또는 기타 문서가 모든 목적을 위해 참조로 통합된다고 개별적으로 표시되는 것과 동일한 정도로 모든 목적을 위해 그 전체가 본원에 참조로 통합된다.

다양한 특정 실시양태가 예시되고 설명되었지만, 본 발명(들)의 사상 및 범위를 벗어나지 않으면서 다양한 변경이 이루어질 수 있음을 이해할 것이다.

SEQUENCE LISTING <110> CODEXIS, INC. <120> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <130> CX2-171WO2 <140> <141> 2019-07-02 <150> 62/695,491 <151> 2018-07-09 <160> 1164 <210> 1 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 2 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 2 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 3 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 3 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 4 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 4 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 5 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 5 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgttt ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 6 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 6 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Val Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 7 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 7 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 8 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 8 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 9 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 9 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 10 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 10 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 11 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 11 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccttgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 12 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 12 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 13 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 13 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct gtaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 14 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 14 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Cys Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 15 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 15 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcgaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctatggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 16 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 16 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Glu Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Tyr Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 17 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 17 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 18 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 18 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 19 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 19 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccgtttct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 20 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 20 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 21 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 21 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 22 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 22 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 23 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 23 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 24 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 24 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 25 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 25 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga aggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 26 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 26 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Glu Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 27 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 27 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctttggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 28 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 28 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Phe Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 29 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 29 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 30 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 30 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 31 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 31 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt gcagacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 32 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 32 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ala Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 33 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 33 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 34 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 34 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 35 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 35 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattacag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 36 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 36 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Thr Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 37 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 37 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattattg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 38 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 38 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Ile Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 39 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 39 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 40 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 40 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 41 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 41 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc atccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 42 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 42 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 43 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 43 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcgcgcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 44 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 44 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Ala Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 45 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 45 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctttggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 46 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 46 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Phe Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 47 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 47 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctatggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 48 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 48 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Tyr Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 49 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 49 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctacat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 50 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 50 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Thr Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 51 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 51 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcgcgcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 52 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 52 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Ala Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 53 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 53 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcgaacgtac cggtaaccgt cacgactatg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 54 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 54 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Glu Arg Thr Gly Asn Arg His Asp Tyr Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 55 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 55 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcgcgcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 56 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 56 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Ala Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 57 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 57 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcgcgcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 58 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 58 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Ala Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 59 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 59 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 60 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 60 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 61 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 61 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 62 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 62 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 63 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 63 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 64 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 64 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ser Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 65 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 65 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc tgcgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 66 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 66 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ala Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 67 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 67 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctatggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 68 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 68 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Tyr Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 69 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 69 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cttggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 70 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 70 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 71 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 71 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt tgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 72 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 72 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Cys Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 73 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 73 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctttggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 74 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 74 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Phe Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 75 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 75 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagtccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 76 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 76 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 77 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 77 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg aagtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 78 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 78 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Glu Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 79 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 79 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctatggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg caatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 80 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 80 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Tyr Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asn Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 81 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 81 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctggtt ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 82 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 82 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Gly Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 83 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 83 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 84 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 84 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 85 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 85 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcgaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctttggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 86 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 86 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Glu Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Phe Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 87 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 87 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgacagg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 88 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 88 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 89 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 89 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgcaggtc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 90 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 90 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 91 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 91 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcgcgcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctttggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 92 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 92 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Ala Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Phe Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 93 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 93 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta accgtcactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 94 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 94 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Arg His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 95 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 95 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 96 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 96 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 97 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 97 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattg gtccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 98 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 98 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Trp Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 99 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 99 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctttggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 100 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 100 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Phe Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 101 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 101 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgactatg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 102 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 102 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Tyr Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 103 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 103 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcgcgcgtac cggtaaccgt cacgacctga gcgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 104 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 104 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Ala Arg Thr Gly Asn Arg His Asp Leu Ser Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 105 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 105 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcgcgcgtac cggtaaccgt cacgacctga gcgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtag cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 106 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 106 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Ala Arg Thr Gly Asn Arg His Asp Leu Ser Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 107 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 107 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 108 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 108 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 109 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 109 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattcagtgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 110 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 110 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Gln Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 111 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 111 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagc gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gtccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 112 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 112 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Ala Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 113 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 113 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgactatg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 114 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 114 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Tyr Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 115 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 115 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattaccg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 116 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 116 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Thr Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 117 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 117 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 118 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 118 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 119 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 119 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 120 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 120 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 121 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 121 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 122 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 122 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 123 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 123 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agcgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 124 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 124 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ser Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 125 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 125 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 126 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 126 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 127 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 127 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattaccg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 128 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 128 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Thr Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 129 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 129 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agcgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 130 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 130 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ser Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 131 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 131 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattaccg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agcgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggacagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 132 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 132 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Thr Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ser Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 133 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 133 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 134 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 134 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 135 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 135 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattaccg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 136 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 136 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Thr Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 137 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 137 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattcagtgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 138 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 138 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Gln Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 139 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 139 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagc gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacgtgt acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 140 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 140 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Ala Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Val Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 141 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 141 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 142 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 142 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 143 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 143 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt gcggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 144 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 144 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ala Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 145 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 145 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 146 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 146 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 147 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 147 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgactatg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 148 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 148 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Tyr Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 149 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 149 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagc gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgactatg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 150 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 150 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Ala Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Tyr Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 151 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 151 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattcagtgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 152 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 152 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Gln Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 153 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 153 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 154 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 154 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 155 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 155 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagc gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gtccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 156 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 156 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Ala Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 157 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 157 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 158 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 158 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 159 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 159 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt tgcgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 160 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 160 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Cys Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 161 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 161 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagc gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgactatg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacgtgt acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 162 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 162 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Ala Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Tyr Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Val Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 163 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 163 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 164 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 164 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 165 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 165 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagc gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gtccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgactatg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 166 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 166 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Ala Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Tyr Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 167 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 167 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattcagtgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 168 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 168 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Gln Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 169 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 169 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 170 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 170 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 171 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 171 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattaccg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt gcggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 172 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 172 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Thr Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ala Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 173 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 173 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agcgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 174 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 174 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ser Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 175 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 175 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattaccg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 176 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 176 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Thr Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 177 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 177 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gtccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 178 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 178 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 179 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 179 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgactatg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacgtgt acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 180 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 180 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Tyr Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Val Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 181 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 181 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagc gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gtccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agcgactatg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 182 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 182 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Ala Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ser Asp Tyr Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 183 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 183 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 184 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 184 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 185 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 185 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 186 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 186 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 187 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 187 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaagc gaccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattg gtccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 188 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 188 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Ala Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Trp Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 189 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 189 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 190 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 190 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 191 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 191 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgactatg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacgtgt acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 192 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 192 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Tyr Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Val Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 193 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 193 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattaccg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 194 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 194 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Thr Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 195 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 195 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 196 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 196 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 197 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 197 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 198 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 198 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 199 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 199 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agcgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 200 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 200 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ser Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 201 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 201 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagc gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattg gtccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 202 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 202 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Ala Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Trp Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 203 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 203 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agcgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 204 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 204 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ser Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 205 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 205 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag tacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt gcggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 206 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 206 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Val 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ala Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 207 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 207 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 208 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 208 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 209 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 209 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagc gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattg gtccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgactatg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacgtgt acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 210 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 210 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Ala Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Trp Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Tyr Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Val Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 211 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 211 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agcgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 212 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 212 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ser Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 213 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 213 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 214 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 214 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 215 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 215 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agcgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 216 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 216 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ser Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 217 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 217 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagc gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacgtgt acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 218 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 218 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Ala Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Val Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 219 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 219 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagc gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacgtgt acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 220 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 220 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Ala Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Val Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 221 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 221 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agcgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 222 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 222 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ser Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 223 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 223 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt gcggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 224 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 224 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ala Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 225 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 225 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaagc gaccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 226 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 226 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Ala Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 227 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 227 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 228 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 228 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 229 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 229 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 230 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 230 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 231 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 231 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 232 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 232 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 233 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 233 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gtccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 234 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 234 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 235 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 235 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 236 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 236 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 237 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 237 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgactatg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 238 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 238 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Tyr Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 239 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 239 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt gcggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 240 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 240 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ala Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 241 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 241 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 242 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 242 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 243 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 243 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattaccg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 244 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 244 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Thr Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 245 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 245 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagc gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattg gtccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgactatg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 246 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 246 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Ala Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Trp Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Tyr Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 247 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 247 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattcagtgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt gcggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 248 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 248 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Gln Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ala Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 249 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 249 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agcgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 250 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 250 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ser Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 251 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 251 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattaccg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt tgcgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 252 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 252 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Thr Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Cys Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 253 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 253 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 254 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 254 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 255 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 255 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 256 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 256 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 257 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 257 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaagc gaccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgactatg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacgtgt acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 258 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 258 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Ala Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Tyr Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Val Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 259 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 259 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 260 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 260 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 261 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 261 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aatgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgactatg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacgtgt acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 262 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 262 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Cys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Tyr Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Val Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 263 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 263 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgacc 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 264 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 264 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Thr Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 265 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 265 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 266 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 266 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 267 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 267 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 268 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 268 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 269 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 269 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattactg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 270 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 270 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Thr Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 271 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 271 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 272 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 272 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 273 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 273 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 274 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 274 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 275 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 275 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 276 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 276 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 277 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 277 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt gcagacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 278 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 278 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ala Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 279 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 279 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 280 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 280 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 281 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 281 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggtatg gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 282 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 282 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Met Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 283 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 283 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc gtccggtacg gtcattactg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 284 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 284 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Thr Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 285 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 285 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattactg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 286 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 286 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Thr Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 287 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 287 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag caactgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 288 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 288 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Thr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 289 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 289 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 290 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 290 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 291 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 291 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctga ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctgggcg caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 292 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 292 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Thr Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Ala His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 293 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 293 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag caccggaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 294 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 294 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Pro Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 295 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 295 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gctatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 296 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 296 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Ala Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 297 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 297 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgactcgacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 298 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 298 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp Ser Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 299 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 299 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt tcagacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 300 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 300 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ser Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 301 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 301 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gcgatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgtcgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 302 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 302 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Ala Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Val Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 303 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 303 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 304 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 304 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 305 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 305 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaacc ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 306 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 306 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Pro Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 307 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 307 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 308 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 308 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 309 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 309 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag tacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa cttttggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt gcagacctgg ctgttgagcc gccagcaccg 720 accgtgctac agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 310 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 310 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Val 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ala Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 311 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 311 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattactg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 312 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 312 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Thr Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 313 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 313 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cagaggaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 314 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 314 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Glu Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 315 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 315 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt gcagacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 316 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 316 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ala Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 317 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 317 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtgt tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 318 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 318 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Val Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 319 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 319 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 320 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 320 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 321 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 321 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctgacaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 322 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 322 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Thr Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 323 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 323 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagt ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 324 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 324 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Val Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 325 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 325 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacac atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccatcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 326 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 326 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Thr Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 His Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 327 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 327 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacgtgt acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 328 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 328 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Val Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 329 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 329 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 330 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 330 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 331 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 331 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaaac gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 332 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 332 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Thr Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 333 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 333 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgc cgcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 334 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 334 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Pro Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 335 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 335 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc gtccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 336 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 336 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 337 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 337 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccctcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 338 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 338 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Pro Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 339 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 339 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 340 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 340 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 341 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 341 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 342 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 342 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 343 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 343 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaaag gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 344 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 344 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Arg Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 345 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 345 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcgtgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 346 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 346 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Val Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 347 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 347 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 ggcgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 348 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 348 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 349 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 349 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 350 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 350 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 351 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 351 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 352 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 352 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 353 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 353 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttacgcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 354 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 354 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Thr Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 355 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 355 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctgg atcaatgggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 356 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 356 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Trp Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 357 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 357 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt cactgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 358 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 358 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 359 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 359 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat gggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 360 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 360 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Met Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 361 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 361 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gtgatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 ggcgacattt acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgt gatccgacct ggaccggtac agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 362 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 362 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 363 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 363 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgttc gggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 364 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 364 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Ser Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 365 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 365 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcgcta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgg gggaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 366 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 366 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Ala Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Gly Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 367 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 367 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cagaagaagg gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcccgcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 368 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 368 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Glu Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Pro Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 369 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 369 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcttc tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 370 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 370 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ser Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 371 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 371 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacggtt acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 372 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 372 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Gly Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 373 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 373 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttaa tggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 374 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 374 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Asn Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 375 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 375 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtcg ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 376 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 376 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Arg Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 377 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 377 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcccgcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgt gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 378 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 378 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Pro Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 379 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 379 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cagaagaagg gtctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcccgcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacgttt acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 380 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 380 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Glu Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Pro Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Val Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 381 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 381 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 ggcgacattt acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 382 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 382 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 383 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 383 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gcgatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 384 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 384 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Ala Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 385 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 385 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccggggcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 386 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 386 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Gly Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 387 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 387 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gtgatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat tggtaaaatt 780 gccgacattt acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgt gatccgacct ggaccggtac agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 388 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 388 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ile Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 389 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 389 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacga gatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 390 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 390 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Glu Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 391 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 391 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 392 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 392 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 393 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 393 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacactctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 394 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 394 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 395 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 395 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctac tggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 396 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 396 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Thr Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 397 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 397 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgtgtc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 398 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 398 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Val Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 399 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 399 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttccgcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 400 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 400 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Pro Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 401 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 401 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtgt gtctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 402 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 402 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Val Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 403 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 403 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcaagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 404 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 404 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Lys Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 405 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 405 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct gcagaaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 406 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 406 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Gln Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 407 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 407 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagagt 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 408 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 408 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu Ser 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 409 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 409 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt ctgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 410 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 410 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Ser Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 411 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 411 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga gagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 412 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 412 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Glu Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 413 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 413 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgcttca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 414 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 414 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Leu 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 415 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 415 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttactcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 416 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 416 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Thr Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 417 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 417 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt cagcgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttcctcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 418 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 418 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Pro Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 419 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 419 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 420 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 420 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 421 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 421 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgccgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgaacc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 422 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 422 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 423 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 423 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgaacc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 424 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 424 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 425 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 425 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgccgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacacactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgaacc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 426 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 426 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 427 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 427 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt cagcgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaatgggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttaa cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttactcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 428 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 428 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Trp Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Asn Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Thr Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Met Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 429 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 429 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt cagcgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 430 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 430 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 431 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 431 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcaaacgtac cggtaaccgt cgtgacctgg ctgttgaacc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 432 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 432 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Lys Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 433 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 433 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt cagcgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgccgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacacactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgaacc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 434 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 434 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 435 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 435 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt agggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 436 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 436 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 437 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 437 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 438 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 438 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 439 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 439 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaatgggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacacactga gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt tcagacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 440 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 440 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Trp Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Ser Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Ser Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 441 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 441 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt cagcgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacacactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgaacc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 442 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 442 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 443 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 443 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgccgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcaaacgtac cggtaaccgt cgtgacctgg ctgttgaacc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 444 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 444 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Lys Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 445 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 445 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt atggacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 446 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 446 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Met Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 447 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 447 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt cagcgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaatgggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttcctcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 448 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 448 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Trp Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Pro Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 449 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 449 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgccgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcaaacgtac cggtaaccgt cgtgacctgg ctgttgaacc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 450 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 450 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Lys Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 451 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 451 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgccgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctga gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttaa cggcgacaaa 660 gccggtaact tcaaacgtac cggtaaccgt cgtgacctgg ctgttactcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctat gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 452 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 452 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Ser Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Asn Gly Asp Lys Ala Gly Asn Phe 210 215 220 Lys Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Thr Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Met Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 453 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 453 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc gtccggtacg gtcattctag atcaattggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgccgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctga gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt agggacctgg ctgttgaacc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 454 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 454 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Ser Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 455 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 455 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggacattt cagcgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgaacc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 456 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 456 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly His Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 457 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 457 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaatgggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgccgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacacactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttaa cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttcctcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 458 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 458 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Trp Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Asn Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Pro Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 459 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 459 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgttc ttccggtacg gtcattctag atcaattggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgccgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttactcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 460 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 460 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Thr Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 461 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 461 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtag ctccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtag cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac atctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 462 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 462 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Ser Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 463 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 463 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cctggacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 464 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 464 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Leu Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 465 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 465 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 466 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 466 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 467 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 467 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctctggagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 468 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 468 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Leu Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 469 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 469 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc ggggcagccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 470 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 470 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Gln Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 471 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 471 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgcctcgtg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 472 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 472 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Ser Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 473 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 473 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaacag gggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 474 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 474 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 475 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 475 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacactggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 476 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 476 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Thr Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 477 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 477 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttccggct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 478 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 478 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Arg Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 479 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 479 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtatggag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 480 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 480 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Met Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 481 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 481 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggatcatctc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 482 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 482 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp His Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 483 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 483 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctggaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 484 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 484 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Trp Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 485 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 485 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 aggaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 486 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 486 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Arg Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 487 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 487 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt cagcgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg cagattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgttttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 488 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 488 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Gln Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 489 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 489 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tcctctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 490 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 490 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ser Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 491 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 491 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa cttggggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 492 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 492 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Trp Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 493 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 493 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtgcg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgt gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 494 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 494 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Ala Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 495 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 495 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat acggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 496 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 496 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Tyr Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 497 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 497 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc taattggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 498 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 498 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Asn Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 499 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 499 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt cagcgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttcctcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 500 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 500 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Pro Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 501 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 501 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 gatgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 502 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 502 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys Asp Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 503 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 503 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcgcg 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 504 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 504 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Ala Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 505 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 505 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa atggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 506 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 506 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Asn Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 507 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 507 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagtt gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 508 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 508 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Leu Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 509 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 509 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gccgggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 510 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 510 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Pro Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 511 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 511 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 ccgaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 512 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 512 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Pro Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 513 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 513 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctattgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 514 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 514 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 515 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 515 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgttatgag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 516 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 516 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Tyr Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 517 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 517 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtag ctccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtag cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt tggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac cgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac atctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 518 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 518 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Ser Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 519 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 519 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccttttg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 520 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 520 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Phe Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 521 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 521 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc ggggaatccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 522 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 522 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Asn Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 523 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 523 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcagtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 524 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 524 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Ser Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 525 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 525 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat ccgtgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 526 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 526 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Arg Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 527 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 527 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaaatgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 528 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 528 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Met Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 529 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 529 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg tttattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 530 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 530 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Phe Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 531 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 531 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggccgtaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 532 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 532 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Arg Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 533 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 533 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgctgag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 534 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 534 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Ala Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 535 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 535 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggccca ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 gtgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 536 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 536 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Val Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 537 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 537 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaagtg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 538 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 538 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Lys Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 539 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 539 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaagcgt tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 540 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 540 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Ala Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 541 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 541 atgaaacgtg catttattat ggtgctggac tcacttggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 542 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 542 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Leu Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 543 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 543 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttactgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 544 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 544 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Thr 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 545 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 545 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccccggcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 546 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 546 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Pro Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 547 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 547 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 cataacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 548 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 548 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His His Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 549 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 549 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtcag gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 550 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 550 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Gln Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 551 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 551 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctcatgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 552 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 552 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala His Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 553 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 553 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtcgggtacc agtattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgcggc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 554 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 554 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Ser Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 555 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 555 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtcgggtagc gtgattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaacac tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 556 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 556 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Ser Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Thr Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 557 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 557 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctcatgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aatttcgttg acttcgacag ctcctggggc cacaggcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 558 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 558 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala His Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 559 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 559 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctcatgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgcggc gggtaaaatt 780 gccgacatct acgccaacac tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aatttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 560 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 560 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala His Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Thr Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 561 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 561 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 562 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 562 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 563 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 563 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctttagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaccg 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 564 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 564 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Leu Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Pro Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 565 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 565 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctcatgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgcggc gggtaaaatt 780 gccgacatct acgccaacac tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgacac ctcctggggc cacaggcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 566 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 566 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala His Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Thr Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Thr Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 567 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 567 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtcgggtacc agtattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctcatgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 568 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 568 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Ser Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala His Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 569 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 569 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtcgggtgtc gggattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 570 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 570 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Val Gly Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 571 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 571 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgtagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 572 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 572 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 573 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 573 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctac caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtcgggtacc gggattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctttagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaccg 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aatttcgttg acttcgactc ttcctggggc cacaggcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 574 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 574 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Gly Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Leu Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Pro Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 575 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 575 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aatttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 576 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 576 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 577 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 577 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtcgggtagc gtgattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaactg tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 578 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 578 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Ser Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 579 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 579 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc ggcgggtacc agtattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgcggc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aatttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 580 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 580 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ala Gly Thr Ser Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 581 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 581 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctttagagtc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgcggc gggtaaaatt 780 gccgacatct acgccaacaa tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 582 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 582 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Leu Glu Ser Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Asn Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 583 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 583 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaacaa tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 584 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 584 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Asn Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 585 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 585 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc ggcgggtacc gggattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctttagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgcggc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 586 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 586 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ala Gly Thr Gly Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Leu Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 587 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 587 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaacac tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 588 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 588 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Thr Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 589 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 589 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgtagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaacaa tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc cacacgcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 590 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 590 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Asn Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Thr Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 591 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 591 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtcgggtacc agtattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaacaa tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 592 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 592 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Ser Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Asn Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 593 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 593 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctcatgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 594 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 594 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala His Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 595 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 595 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtcgggtgtc gggattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctttagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaccg 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aatttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 596 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 596 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Val Gly Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Leu Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Pro Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 597 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 597 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgacac ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 598 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 598 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Thr Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 599 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 599 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gggattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 600 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 600 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Gly Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 601 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 601 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtcgggtacc agtattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctcatgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgcggc gggtaaaatt 780 gccgacatct acgcccacaa tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 602 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 602 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Ser Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala His Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala His Asn Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 603 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 603 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgccccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtcgggtacc gtgattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctttagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaacac tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 604 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 604 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Ala Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Leu Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Thr Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 605 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 605 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtcgggtacc gtgattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaacaa tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 606 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 606 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Asn Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 607 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 607 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtcgggtgtc gtgattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaacaa tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 608 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 608 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Val Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Asn Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 609 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 609 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc ggcgggtacc agtattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctttagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 610 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 610 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ala Gly Thr Ser Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Leu Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 611 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 611 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc ggcgggtagc gtgattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc cacaggcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 612 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 612 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ala Gly Ser Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 613 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 613 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaccg 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 614 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 614 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Pro Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 615 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 615 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaacac gggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 616 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 616 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Thr Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 617 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 617 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc cacactcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 618 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 618 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Thr Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 619 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 619 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgtagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgcggc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aatttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 620 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 620 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 621 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 621 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgtttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 622 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 622 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 623 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 623 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtcgggtacc gggattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgcggc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aatttcgttg acttcgacag ctcctggggc cacaggcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 624 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 624 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Gly Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 625 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 625 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaggcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 626 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 626 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 627 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 627 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 628 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 628 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 629 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 629 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 630 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 630 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 631 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 631 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 632 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 632 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 633 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 633 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcgg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 634 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 634 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 635 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 635 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacccg 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 636 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 636 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Pro Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 637 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 637 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 638 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 638 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 639 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 639 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atgcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 640 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 640 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Ala Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 641 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 641 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 642 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 642 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 643 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 643 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag attctctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 644 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 644 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Ser Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 645 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 645 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 646 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 646 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 647 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 647 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatacg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 648 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 648 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Thr Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 649 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 649 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatggg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 650 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 650 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Gly Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 651 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 651 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ccgggatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 652 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 652 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Arg Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 653 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 653 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg ctgattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 654 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 654 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Leu Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 655 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 655 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 656 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 656 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 657 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 657 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 658 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 658 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 659 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 659 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 660 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 660 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 661 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 661 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 662 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 662 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 663 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 663 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 664 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 664 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 665 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 665 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 666 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 666 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 667 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 667 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgggtggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 668 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 668 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Gly Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 669 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 669 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atgcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 670 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 670 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Ala Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 671 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 671 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 672 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 672 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 673 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 673 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcaccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 674 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 674 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Thr Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 675 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 675 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgggtggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 676 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 676 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Gly Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 677 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 677 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcacg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 678 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 678 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Thr Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 679 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 679 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 680 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 680 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 681 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 681 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atgcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 682 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 682 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Ala Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 683 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 683 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 684 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 684 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 685 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 685 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gaccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 686 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 686 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 687 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 687 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gcatatggca aagccatgtt c 1221 <210> 688 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 688 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Ala Tyr Gly Lys Ala Met Phe 405 <210> 689 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 689 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 690 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 690 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 691 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 691 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag attcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 692 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 692 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Ser Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 693 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 693 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg accattctag attcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 694 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 694 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Ser Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 695 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 695 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgaccct 660 gccgcgaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 696 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 696 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Pro Ala Ala Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 697 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 697 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgggtggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 698 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 698 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Gly Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 699 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 699 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg accattctag attcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 700 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 700 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Ser Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 701 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 701 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gctgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 702 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 702 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 703 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 703 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gaccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 704 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 704 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 705 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 705 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gaccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atgcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 706 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 706 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Ala Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 707 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 707 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag attcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 708 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 708 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Ser Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 709 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 709 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag attcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 710 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 710 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Ser Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 711 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 711 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgggtggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcacagctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 712 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 712 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Gly Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 713 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 713 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atgcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 714 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 714 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Ala Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 715 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 715 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 716 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 716 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 717 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 717 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgggtggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 718 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 718 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Gly Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 719 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 719 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgaccct 660 gccgggaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 720 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 720 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Pro Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 721 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 721 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgggtggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 722 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 722 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Gly Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 723 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 723 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gaccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg accattctag attcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 724 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 724 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Ser Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 725 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 725 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgggtggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 726 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 726 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Gly Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 727 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 727 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 728 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 728 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 729 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 729 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gaccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 730 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 730 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 731 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 731 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 732 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 732 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 733 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 733 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 734 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 734 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 735 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 735 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgggtggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 736 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 736 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Gly Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 737 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 737 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gaccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgggtggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 738 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 738 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Gly Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 739 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 739 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 740 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 740 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 741 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 741 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atgcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 742 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 742 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Ala Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 743 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 743 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atgcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 744 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 744 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Ala Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 745 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 745 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gaccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 746 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 746 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 747 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 747 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 748 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 748 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 749 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 749 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atgcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 750 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 750 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Ala Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 751 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 751 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gaccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atgcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 752 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 752 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Ala Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 753 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 753 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 754 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 754 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 755 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 755 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gaccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgaccct 660 gccgggaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 756 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 756 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Pro Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 757 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 757 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 758 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 758 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 759 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 759 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgaccct 660 gccgggaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 760 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 760 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Pro Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 761 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 761 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgacgg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag attcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 762 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 762 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Asp Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Ser Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 763 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 763 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gaccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 764 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 764 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 765 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 765 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 tggcgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gaccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgggtggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 766 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 766 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Trp Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Gly Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 767 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 767 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgggtggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 768 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 768 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Gly Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 769 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 769 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg accattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 770 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 770 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 771 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 771 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg accattctag attcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 772 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 772 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Ser Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 773 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 773 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gaccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atgcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgcgaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 774 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 774 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Trp Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Ala Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ala Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 775 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 775 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccaggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgaccct 660 gccgggaact tccagcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 776 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 776 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Pro Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 777 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 777 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag attcgctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccgggaact tccgtcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 778 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 778 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Ser Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 779 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 779 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat catcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 780 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 780 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Ile Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 781 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 781 atgaaacgtg catttattat gactctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat tggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 782 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 782 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 783 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 783 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactgctgg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 784 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 784 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Leu Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 785 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 785 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacaagcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 786 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 786 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Lys Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 787 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 787 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tacgccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 788 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 788 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Thr Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 789 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 789 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 790 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 790 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 791 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 791 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaaatg 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 792 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 792 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Met Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 793 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 793 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaagttgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 794 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 794 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Val Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 795 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 795 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggt gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 796 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 796 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 797 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 797 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgatctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 798 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 798 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Asp 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 799 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 799 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgttataaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 800 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 800 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Tyr Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 801 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 801 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 802 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 802 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 803 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 803 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggttt gtctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 804 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 804 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Leu Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 805 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 805 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 806 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 806 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 807 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 807 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtattgggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 808 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 808 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Leu Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 809 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 809 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacatgcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 810 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 810 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Met Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 811 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 811 atgaaacgtg catttatgat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 812 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 812 Met Lys Arg Ala Phe Met Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 813 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 813 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact gctgtatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 814 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 814 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Leu 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 815 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 815 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 gtgctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 816 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 816 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Val Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 817 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 817 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg cagattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 818 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 818 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Gln Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 819 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 819 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcaatcgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 820 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 820 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Asn Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 821 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 821 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagagt 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 822 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 822 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu Ser 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 823 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 823 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 824 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 824 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 825 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 825 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg tgaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 826 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 826 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Val Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 827 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 827 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact attgcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 828 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 828 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Ile Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 829 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 829 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaagcggtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 830 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 830 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Ala 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 831 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 831 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttgt gggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 832 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 832 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Val Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 833 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 833 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataactcgcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 834 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 834 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Ser Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 835 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 835 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa gctggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 836 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 836 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys Ala Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 837 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 837 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagctcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 838 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 838 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Ala Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 839 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 839 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctacgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 840 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 840 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Thr Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 841 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 841 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggtgccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 842 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 842 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Val Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 843 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 843 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacacgctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 844 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 844 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 845 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 845 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 846 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 846 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 847 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 847 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccgccgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtactgggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 848 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 848 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Leu Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 849 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 849 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaagccgtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 850 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 850 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Ala 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 851 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 851 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 852 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 852 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 853 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 853 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccgccgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 854 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 854 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 855 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 855 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaagccgtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 856 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 856 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Ala 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 857 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 857 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtag tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtactgggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtct gtctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 858 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 858 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Ser Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Leu Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Leu Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 859 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 859 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtag tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtct gtctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 860 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 860 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Ser Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Leu Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 861 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 861 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 862 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 862 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 863 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 863 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaagccgtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtct gtctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 864 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 864 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Ala 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Leu Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 865 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 865 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccgccgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtct gtctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 866 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 866 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Leu Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 867 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 867 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaagccgtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 868 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 868 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Ala 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 869 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 869 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 870 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 870 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 871 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 871 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtag tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtactgggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 872 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 872 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Ser Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Leu Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 873 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 873 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 874 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 874 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 875 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 875 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 876 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 876 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 877 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 877 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccgccgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 878 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 878 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 879 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 879 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtactgggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 880 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 880 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Leu Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 881 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 881 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 882 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 882 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 883 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 883 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccgccgtag tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaagccgtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtactgggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 884 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 884 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Ser Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Ala 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Leu Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 885 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 885 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtag tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaagccgtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 886 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 886 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Ser Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Ala 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 887 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 887 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaagccgtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtactgggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 888 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 888 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Ala 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Leu Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 889 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 889 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtactgggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 890 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 890 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Leu Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 891 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 891 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaagccgtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 892 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 892 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Ala 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 893 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 893 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtct gtctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 894 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 894 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Leu Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 895 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 895 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 896 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 896 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 897 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 897 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccgccgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaagccgtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 898 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 898 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Ala 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 899 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 899 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 900 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 900 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 901 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 901 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 902 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 902 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 903 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 903 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 904 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 904 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 905 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 905 atgaaacgtg catttattat ggtgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 906 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 906 Met Lys Arg Ala Phe Ile Met Val Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 907 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 907 atgaaacgtg catttattat gcttctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 908 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 908 Met Lys Arg Ala Phe Ile Met Leu Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 909 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 909 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaggcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 910 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 910 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 911 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 911 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 912 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 912 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 913 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 913 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 914 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 914 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 915 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 915 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 916 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 916 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 917 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 917 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca agaccacacg 1080 cgtgaacaca ttccggtact ggtactgggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 918 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 918 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Leu Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 919 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 919 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 920 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 920 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 921 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 921 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtgt ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 922 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 922 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Val Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 923 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 923 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggttc ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 924 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 924 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Ser Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 925 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 925 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaagccgtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 926 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 926 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Ala 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 927 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 927 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagctattct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 928 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 928 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Tyr Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 929 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 929 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatgag 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 930 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 930 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Glu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 931 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 931 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccgccgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 932 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 932 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 933 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 933 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg cttcggagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 934 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 934 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 935 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 935 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 936 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 936 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 937 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 937 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtag tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaagccgtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtactgggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 938 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 938 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Ser Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Ala 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Leu Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 939 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 939 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc ggtggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 940 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 940 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Val Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 941 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 941 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaagccgtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 942 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 942 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Ala 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 943 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 943 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccgccgtag tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 944 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 944 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Ser Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 945 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 945 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatcgc 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccgccgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaagccgtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 946 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 946 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Ala 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 947 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 947 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgctgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccgccgtag tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 948 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 948 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Cys Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Arg Arg Ser Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 949 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 949 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 950 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 950 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 951 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 951 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtag tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcgct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 952 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 952 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Ser Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 953 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 953 atgaaacgtg catttattat gtcgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 954 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 954 Met Lys Arg Ala Phe Ile Met Ser Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 955 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 955 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtat ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 956 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 956 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Met Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 957 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 957 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaagccgtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 958 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 958 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Ala 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 959 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 959 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtct gtctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 960 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 960 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Leu Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 961 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 961 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca ggaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 962 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 962 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Gln Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 963 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 963 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggt cggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 964 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 964 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 965 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 965 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg tgcaaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 966 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 966 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ala Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 967 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 967 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cactgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 968 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 968 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 969 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 969 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tacgccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc ggtggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 970 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 970 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Thr Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Val Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 971 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 971 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctgg cctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 972 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 972 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Gly Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 973 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 973 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggactgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 974 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 974 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Leu Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 975 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 975 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 976 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 976 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 977 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 977 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaacctatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 978 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 978 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Pro Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 979 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 979 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa ggcggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 980 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 980 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys Gly Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 981 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 981 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggccaggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 982 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 982 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Pro Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 983 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 983 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact gtcgcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 984 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 984 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 985 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 985 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaaaaatttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 986 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 986 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 987 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 987 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 988 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 988 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 989 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 989 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggatcgtctc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 990 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 990 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Arg Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 991 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 991 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttgt gggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 992 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 992 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Val Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 993 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 993 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttagtct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 994 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 994 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 995 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 995 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggacgggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 996 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 996 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 997 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 997 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 998 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 998 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 999 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 999 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc ggtggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1000 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1000 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Val Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1001 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1001 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tcaaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1002 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1002 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Lys Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1003 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1003 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggaaaaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1004 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1004 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1005 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1005 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1006 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1006 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1007 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1007 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1008 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1008 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1009 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1009 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttagtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1010 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1010 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1011 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1011 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1012 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1012 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1013 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1013 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgaaaggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1014 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1014 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Lys Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1015 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1015 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttagtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1016 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1016 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1017 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1017 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttagtct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1018 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1018 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1019 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1019 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1020 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1020 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1021 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1021 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tacgccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1022 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1022 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Thr Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1023 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1023 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 ccaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1024 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1024 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Pro Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1025 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1025 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcggcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1026 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1026 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1027 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1027 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1028 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1028 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1029 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1029 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tacgccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1030 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1030 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Thr Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1031 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1031 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc ggtggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttagtct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggacgggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1032 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1032 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Val Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1033 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1033 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1034 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1034 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1035 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1035 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1036 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1036 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1037 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1037 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttagtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1038 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1038 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1039 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1039 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggacgggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1040 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1040 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1041 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1041 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggacgggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1042 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1042 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1043 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1043 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tcctgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1044 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1044 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Pro Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1045 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1045 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tacgccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc ggtggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1046 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1046 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Thr Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Val Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1047 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1047 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgccgcg 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1048 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1048 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1049 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1049 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gagattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1050 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1050 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Glu Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1051 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1051 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1052 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1052 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1053 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1053 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tacgccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1054 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1054 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Thr Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1055 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1055 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atgaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1056 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1056 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Glu Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1057 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1057 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgg gtgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1058 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1058 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Gly Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1059 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1059 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcga agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1060 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1060 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Glu Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1061 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1061 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgctgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1062 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1062 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Leu Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1063 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1063 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaat acttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1064 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1064 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Tyr Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1065 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1065 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgaagga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1066 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1066 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Lys Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1067 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1067 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1068 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1068 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1069 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1069 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctgggcaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1070 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1070 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Gly Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1071 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1071 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggaag aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1072 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1072 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Glu Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1073 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1073 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1074 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1074 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1075 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1075 atgaaacgtg catttattat cacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1076 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1076 Met Lys Arg Ala Phe Ile Ile Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1077 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1077 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaactgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1078 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1078 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Leu Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1079 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1079 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt catgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1080 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1080 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1081 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1081 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgaa ggatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1082 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1082 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Lys Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1083 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1083 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1084 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1084 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1085 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1085 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc ggtggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtct gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1086 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1086 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Val Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1087 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1087 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttagtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1088 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1088 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1089 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1089 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1090 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1090 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1091 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1091 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttagtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1092 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1092 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1093 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1093 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgacgc acgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1094 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1094 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Ala Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1095 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1095 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccctgaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1096 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1096 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Leu Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1097 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1097 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacagtat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1098 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1098 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Ser Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1099 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1099 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac caaactgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1100 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1100 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Lys Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1101 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1101 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc ggtggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1102 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1102 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Val Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1103 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1103 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1104 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1104 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1105 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1105 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttagtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1106 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1106 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1107 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1107 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc ggtggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1108 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1108 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Val Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1109 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1109 atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tacgccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc ggtggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttagtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1110 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1110 Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Thr Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Val Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1111 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1111 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttagtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggacgggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1112 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1112 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1113 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1113 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agctatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1114 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1114 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Ala 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1115 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1115 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gaggtgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1116 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1116 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Val Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1117 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1117 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1118 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1118 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1119 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1119 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaataca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1120 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1120 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu Tyr Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1121 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1121 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctgc cgaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1122 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1122 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Ala Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1123 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1123 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggcgcgact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1124 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1124 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Ala Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1125 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1125 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgaaaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1126 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1126 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Glu Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1127 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1127 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctgcagaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1128 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1128 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Ala Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1129 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1129 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cccggacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1130 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1130 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Pro Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1131 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1131 atgaaacgtg catttctgat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1132 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1132 Met Lys Arg Ala Phe Leu Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1133 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1133 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaaaaatttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctgacgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1134 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1134 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Thr Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1135 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1135 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaaatc 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1136 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1136 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Ile Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1137 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1137 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaaggtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1138 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1138 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Lys Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1139 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1139 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1140 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1140 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1141 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1141 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctaaactgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1142 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1142 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Lys Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1143 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1143 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ttggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1144 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1144 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Phe Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1145 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1145 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tacgccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc ggtggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1146 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1146 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Thr Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Val Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1147 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1147 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tacgccggtt ctgtttgaga tgggatattt tacggatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacaccctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc ggtggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggcgcggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1148 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1148 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Thr Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Val Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Arg Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1149 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1149 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatagagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcagagt attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1150 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1150 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Glu Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1151 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1151 atgaaacgtg catttattat gacgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taccgatctg 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctagtgagcc gggggggccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttagtgc gggtaaaatt 780 gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggacgggtga agaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1152 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES <400> 1152 Met Lys Arg Ala Phe Ile Met Thr Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ser Glu Pro Gly Gly Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1153 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Codon optimized Purine Nucleoside Phosphorylase from E. Coli <400> 1153 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 1154 <211> 239 <212> PRT <213> Escherichia coli <220> <223> Purine Nucleoside Phosphorylase from E. Coli <400> 1154 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 1155 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 1155 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 1156 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 1156 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 1157 <211> 1500 <212> DNA <213> Artificial Sequence <220> <223> Variant of Sucrose phosphorylase from Alloscardovia omnicolens <400> 1157 atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60 agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120 ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180 gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240 atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300 gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360 gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420 acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480 gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540 ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600 aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660 gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720 gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780 catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840 aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900 cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960 agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020 ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080 tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140 ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200 atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260 caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320 agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380 accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440 accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500 <210> 1158 <211> 500 <212> PRT <213> Artificial Sequence <220> <223> Variant of Sucrose phosphorylase from Alloscardovia omnicolens <400> 1158 Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 1 5 10 15 Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 20 25 30 Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 35 40 45 Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 50 55 60 Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 65 70 75 80 Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 85 90 95 Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 100 105 110 Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 115 120 125 Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 130 135 140 Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 145 150 155 160 Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 165 170 175 Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 180 185 190 Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 195 200 205 Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 210 215 220 Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 225 230 235 240 Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 245 250 255 Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 260 265 270 Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 275 280 285 His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 290 295 300 Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 305 310 315 320 Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 325 330 335 Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 340 345 350 Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 355 360 365 Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 370 375 380 Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 385 390 395 400 Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 405 410 415 Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 420 425 430 Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 435 440 445 Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 450 455 460 Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 465 470 475 480 Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 485 490 495 Pro Val Val Ala 500 <210> 1159 <211> 1500 <212> DNA <213> Artificial Sequence <220> <223> Variant of Sucrose phosphorylase from Alloscardovia omnicolens <400> 1159 atgaagaaca aagttcaact gattacctat gcggatcgtc tgggtgacgg caccctgaag 60 agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120 ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180 gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240 atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300 gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360 gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420 acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccgtcagcaa 480 gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540 ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600 aaaaacagca gctgcttcct gaccccgaag acctttaaac tggtcgaacg tattaaggcg 660 gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720 gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780 catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840 aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900 cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960 agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020 ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080 tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140 ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200 atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260 caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320 agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380 accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440 accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500 <210> 1160 <211> 500 <212> PRT <213> Artificial Sequence <220> <223> Variant of Sucrose phosphorylase from Alloscardovia omnicolens <400> 1160 Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 1 5 10 15 Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 20 25 30 Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 35 40 45 Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 50 55 60 Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 65 70 75 80 Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 85 90 95 Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 100 105 110 Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 115 120 125 Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 130 135 140 Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Arg Gln Gln 145 150 155 160 Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 165 170 175 Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 180 185 190 Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Leu Thr 195 200 205 Pro Lys Thr Phe Lys Leu Val Glu Arg Ile Lys Ala Glu Gly Glu Lys 210 215 220 Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 225 230 235 240 Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 245 250 255 Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 260 265 270 Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 275 280 285 His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 290 295 300 Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 305 310 315 320 Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 325 330 335 Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 340 345 350 Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 355 360 365 Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 370 375 380 Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 385 390 395 400 Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 405 410 415 Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 420 425 430 Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 435 440 445 Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 450 455 460 Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 465 470 475 480 Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 485 490 495 Pro Val Val Ala 500 <210> 1161 <211> 771 <212> DNA <213> Artificial Sequence <220> <223> Variant of Deoxyribose Phosphate Aldolase From Shewanella Halifaxensis <400> 1161 atgtgtgatc tgaagaaggc ggcgcagcgg gcgattagcc tgatggatct gaccaccctg 60 aacgacgatg acaccgacca gaaagtgatt gagctgtgcc acaaagcgaa aaccccggcg 120 ggtgacaccg cggcgatcgt gatttacccg cgtttcatcc cgattgcgcg taagaccctg 180 aacgaaatcg gtggcctgga catcaaaatt gtcaccgtta ccaactttcc gcacggtaac 240 gatgacattg cgattgcggt gctggagacc cgtgcggcgg ttgcgtacgg cgcggatgaa 300 gtggacgtgg ttttcccgta tcgtgcgctg atggaaggta acgaaaccgt gggctttgag 360 ctggttaagg cgtgcaaaga ggcgtgcggt gaagatacca ttctgaaggt gatcattgaa 420 agtggcgtac tgaaagaccc ggcgctgatt cgtaaagcga gcgaaattag catcgatgcg 480 ggtgcggact tcattaagac cagcaccggc aaagttgcgg ttaacgcgac cctggaggcg 540 gcggaaatca ttatgaccgt tattagcgag aagaacccga aagtgggttt caagccggcg 600 ggtggcatta aagatgctgc ggcggcggcg gaatttctgg gtgtggcggc gcgtctgctg 660 ggcgatgact gggcgacccc ggcgaccttc cgttttggtg cgaccgacct gctgaccaac 720 ctgctgcaca ccctggaact ggcggacgcg ccgcagggtg cgcaaggcta t 771 <210> 1162 <211> 257 <212> PRT <213> Artificial Sequence <220> <223> Variant of Deoxyribose Phosphate Aldolase From Shewanella Halifaxensis <400> 1162 Met Cys Asp Leu Lys Lys Ala Ala Gln Arg Ala Ile Ser Leu Met Asp 1 5 10 15 Leu Thr Thr Leu Asn Asp Asp Asp Thr Asp Gln Lys Val Ile Glu Leu 20 25 30 Cys His Lys Ala Lys Thr Pro Ala Gly Asp Thr Ala Ala Ile Val Ile 35 40 45 Tyr Pro Arg Phe Ile Pro Ile Ala Arg Lys Thr Leu Asn Glu Ile Gly 50 55 60 Gly Leu Asp Ile Lys Ile Val Thr Val Thr Asn Phe Pro His Gly Asn 65 70 75 80 Asp Asp Ile Ala Ile Ala Val Leu Glu Thr Arg Ala Ala Val Ala Tyr 85 90 95 Gly Ala Asp Glu Val Asp Val Val Phe Pro Tyr Arg Ala Leu Met Glu 100 105 110 Gly Asn Glu Thr Val Gly Phe Glu Leu Val Lys Ala Cys Lys Glu Ala 115 120 125 Cys Gly Glu Asp Thr Ile Leu Lys Val Ile Ile Glu Ser Gly Val Leu 130 135 140 Lys Asp Pro Ala Leu Ile Arg Lys Ala Ser Glu Ile Ser Ile Asp Ala 145 150 155 160 Gly Ala Asp Phe Ile Lys Thr Ser Thr Gly Lys Val Ala Val Asn Ala 165 170 175 Thr Leu Glu Ala Ala Glu Ile Ile Met Thr Val Ile Ser Glu Lys Asn 180 185 190 Pro Lys Val Gly Phe Lys Pro Ala Gly Gly Ile Lys Asp Ala Ala Ala 195 200 205 Ala Ala Glu Phe Leu Gly Val Ala Ala Arg Leu Leu Gly Asp Asp Trp 210 215 220 Ala Thr Pro Ala Thr Phe Arg Phe Gly Ala Thr Asp Leu Leu Thr Asn 225 230 235 240 Leu Leu His Thr Leu Glu Leu Ala Asp Ala Pro Gln Gly Ala Gln Gly 245 250 255 Tyr <210> 1163 <211> 771 <212> DNA <213> Artificial Sequence <220> <223> Variant of Deoxyribose Phosphate Aldolase From Shewanella Halifaxensis <400> 1163 atgtgcgatc tgaagaaggc agcgcagcgg gcgattagcc tgatggatct gaccaccctg 60 aacgacgatg acaccgacca gaaagtgatt gagctgtgcc acaaagcgaa aaccccggcg 120 ggtgacaccg cggcgatcgt gatttacccg cgtttcatcc cgattgcgcg taagaccctg 180 aacgaaatcg gtggcctgga catcaaaatt gccaccgtta ccaactttcc gcacggtaac 240 gatgacattg cgattgcggt gctggagacc cgtgcggcgg ttgcgtacgg cgcggatgaa 300 gtggacgtgg ttttcccgta tcgtgcgctg atggaaggta acgaaaccgt gggctttgag 360 ctggttaagg cgtgcaaaga ggcgtgcggt gaagatcata ttctgaaggt gatcattgaa 420 agtggcgtac tgaaagaccc ggcgctgatt cgtaaagcga gcgaaattag catcgatgcg 480 ggtgcggact tcattaagac cagcaccggc aaagttgcgg ttaacgcgac cctggaggcg 540 gcggaaatca ttatgaccgt tattagcgag aagaacccga aagtgggttt caagccggcg 600 ggtggcatta aagatgctgc ggcggcggcg gaatttctgg gtgtggcggc gcgtctgctg 660 ggcgatgact gggcgacccc ggcgaccttc cgttttggtg cgaccgacct gctgaccaac 720 ctgctgcaca ccctggaact ggcggacgcg ccgcagggtg cgcaaggcta t 771 <210> 1164 <211> 257 <212> PRT <213> Artificial Sequence <220> <223> Variant of Deoxyribose Phosphate Aldolase From Shewanella Halifaxensis <400> 1164 Met Cys Asp Leu Lys Lys Ala Ala Gln Arg Ala Ile Ser Leu Met Asp 1 5 10 15 Leu Thr Thr Leu Asn Asp Asp Asp Thr Asp Gln Lys Val Ile Glu Leu 20 25 30 Cys His Lys Ala Lys Thr Pro Ala Gly Asp Thr Ala Ala Ile Val Ile 35 40 45 Tyr Pro Arg Phe Ile Pro Ile Ala Arg Lys Thr Leu Asn Glu Ile Gly 50 55 60 Gly Leu Asp Ile Lys Ile Ala Thr Val Thr Asn Phe Pro His Gly Asn 65 70 75 80 Asp Asp Ile Ala Ile Ala Val Leu Glu Thr Arg Ala Ala Val Ala Tyr 85 90 95 Gly Ala Asp Glu Val Asp Val Val Phe Pro Tyr Arg Ala Leu Met Glu 100 105 110 Gly Asn Glu Thr Val Gly Phe Glu Leu Val Lys Ala Cys Lys Glu Ala 115 120 125 Cys Gly Glu Asp His Ile Leu Lys Val Ile Ile Glu Ser Gly Val Leu 130 135 140 Lys Asp Pro Ala Leu Ile Arg Lys Ala Ser Glu Ile Ser Ile Asp Ala 145 150 155 160 Gly Ala Asp Phe Ile Lys Thr Ser Thr Gly Lys Val Ala Val Asn Ala 165 170 175 Thr Leu Glu Ala Ala Glu Ile Ile Met Thr Val Ile Ser Glu Lys Asn 180 185 190 Pro Lys Val Gly Phe Lys Pro Ala Gly Gly Ile Lys Asp Ala Ala Ala 195 200 205 Ala Ala Glu Phe Leu Gly Val Ala Ala Arg Leu Leu Gly Asp Asp Trp 210 215 220 Ala Thr Pro Ala Thr Phe Arg Phe Gly Ala Thr Asp Leu Leu Thr Asn 225 230 235 240 Leu Leu His Thr Leu Glu Leu Ala Asp Ala Pro Gln Gly Ala Gln Gly 245 250 255 Tyr

Claims

서열 번호 2, 4, 118, 266, 420, 562, 656, 790 및/또는 846에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열, 또는 이의 기능적 단편을 포함하는 조작된 포스포펜토뮤타제로서, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열은 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 2, 4, 118, 266, 420, 562, 656, 790, 및/또는 846을 기준으로 넘버링되는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열은

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 2를 기준으로 넘버링되는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제의 상기 폴리펩타이드 서열이 서열 번호 4에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 포스포펜토뮤타제의 상기 폴리펩타이드 서열은

으로부터 선택되는 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 4를 기준으로 넘버링되는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열이 서열 번호 118에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열은

로부터 선택되는 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 118를 기준으로 넘버링되는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열이 서열 번호 266에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열은 65/225, 65/225/263, 97, 118, 143/194, 147, 156, 160, 172, 176, 188, 192, 194, 207, 211, 217, 225, 227, 236, 238, 257, 257/261, 261, 263, 306, 및 397로부터 선택되는 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 266을 기준으로 넘버링되는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열이 서열 번호 266에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열은

로부터 선택되는 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 266을 기준으로 넘버링되는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열이 서열 번호 420에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열은 12, 94, 118/147/227/238/257/357, 118/151, 118/236/357, 121, 142, 148, 150, 151, 172, 181, 185, 186, 189, 193, 199, 206, 218, 235, 237, 239, 256, 266, 267, 281, 300, 308, 및 383으로부터 선택되는 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 420을 기준으로 넘버링되는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열이 서열 번호 420에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열은

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 420을 기준으로 넘버링되는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열이 서열 번호 562에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열은 21, 118, 120, 151, 155, 220, 225 및 357로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 562를 기준으로 넘버링되는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열이 서열 번호 562에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열은

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 562를 기준으로 넘버링되는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열이 서열 번호 656에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열은 6, 8, 39, 46, 53, 108, 133, 151, 160, 192, 196, 200, 217, 225, 236, 239, 251, 257, 272, 284, 335, 341, 368, 369, 391, 및 397로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 656을 기준으로 넘버링되는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열이 서열 번호 790에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열은

357, 및 357/397로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 790을 기준으로 넘버링되는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열이 서열 번호 846에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 포스포펜토뮤타제의 폴리펩타이드 서열은 6, 7,

으로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 846을 기준으로 넘버링되는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제가 표 4.1, 5.1, 6.1, 7.1, 8.1, 9.1, 10.1, 12.1, 12.2, 13.1, 14.1 및/또는 15.1에 제시된 적어도 하나의 조작된 포스포펜토뮤타제 변이체의 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과로 동일한 폴리펩타이드 서열을 포함하는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제가 서열 번호 2, 4, 118, 266, 420, 562, 656, 790 및/또는 846에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과로 동일한 폴리펩타이드 서열을 포함하는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제가 서열 번호 4, 118, 266, 420, 562, 656, 790, 및/또는 846에 제시된 조작된 포스포펜토뮤타제 변이체를 포함하는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제가 서열 번호 8-1152의 짝수 번호 서열에 제시된 적어도 하나의 조작된 포스포펜토뮤타제 변이체의 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과로 동일한 폴리펩타이드 서열을 포함하는 것인 조작된 포스포펜토뮤타제.
제1항에 있어서, 상기 조작된 포스포펜토뮤타제가 서열 번호 8-1152의 짝수 번호 서열 중 적어도 하나에 제시된 폴리펩타이드 서열을 포함하는 것인 조작된 포스포펜토뮤타제.
제1항 내지 제18항 중 어느 한 항에 있어서, 상기 조작된 포스포펜토뮤타제가 야생형 이. 콜라이(E. coli) 포스포펜토뮤타제에 비해 적어도 하나의 개선된 특성을 포함하는 것인 조작된 포스포펜토뮤타제.
제19항에 있어서, 상기 개선된 특성이 기질에 대한 개선된 활성을 포함하는 것인 조작된 포스포펜토뮤타제.
제15항에 있어서, 상기 기질이 화합물 4를 포함하는 것인 조작된 포스포펜토뮤타제.
제1항 내지 제21항 중 어느 한 항에 있어서, 상기 개선된 특성이 화합물 1 및/또는 화합물 3의 개선된 생산을 포함하는 것인 조작된 포스포펜토뮤타제.
제1항 내지 제22항 중 어느 한 항에 있어서, 상기 조작된 포스포펜토뮤타제가 정제된 것인 조작된 포스포펜토뮤타제.
제1항 내지 제23항 중 어느 한 항의 적어도 하나의 조작된 포스포펜토뮤타제를 포함하는 조성물.
제1항 내지 제23항 중 어느 한 항의 적어도 하나의 조작된 포스포펜토뮤타제를 코딩하는 폴리뉴클레오타이드 서열.
적어도 하나의 조작된 포스포펜토뮤타제를 코딩하는 폴리뉴클레오타이드 서열로서, 상기 폴리뉴클레오타이드 서열이 서열 번호 1, 3, 117, 265, 419, 561, 655, 789 및/또는 845에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 포함하고, 상기 조작된 포스포펜토뮤타제의 폴리뉴클레오타이드 서열이 하나 이상의 위치에 적어도 하나의 치환을 포함하는 것인 폴리뉴클레오타이드 서열.
서열 번호 1, 3, 117, 265, 419, 561, 655, 789 및/또는 845에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 포함하는 적어도 하나의 조작된 포스포펜토뮤타제, 또는 이의 기능적 단편을 코딩하는 폴리뉴클레오타이드 서열.
제25항 내지 제27항 중 어느 한 항에 있어서, 제어 서열에 작동 가능하게 연결되는 것인 폴리뉴클레오타이드 서열.
제25항 내지 제28항 중 어느 한 항에 있어서, 코돈 최적화되는 것인 폴리뉴클레오타이드 서열.
제25항 내지 제29항 중 어느 한 항에 있어서, 서열 번호 7-1151의 홀수 번호 서열에 제시된 폴리뉴클레오타이드 서열을 포함하는 것인 폴리뉴클레오타이드 서열.
제25항 내지 제30항 중 어느 한 항의 적어도 하나의 폴리뉴클레오타이드 서열을 포함하는 발현 벡터.
제31항의 적어도 하나의 발현 벡터를 포함하는 숙주 세포.
제25항 내지 제30항 중 어느 한 항의 적어도 하나의 폴리뉴클레오타이드 서열을 포함하는 숙주 세포.
적어도 하나의 조작된 포스포펜토뮤타제가 생산되도록 적절한 조건 하에 제32항 및/또는 제33항의 숙주 세포를 배양하는 단계를 포함하는, 숙주 세포에서 조작된 포스포펜토뮤타제를 생산하는 방법.
제34항에 있어서, 배양액 및/또는 숙주 세포로부터 적어도 하나의 조작된 포스포펜토뮤타제를 회수하는 단계를 추가로 포함하는 방법.
제34항 및/또는 제35항에 있어서, 상기 적어도 하나의 조작된 포스포펜토뮤타제를 정제하는 단계를 추가로 포함하는 방법.