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ATE264386T1 - Verfahren zur rnase- und organische lösemitteln- freier reinigung von plasmid-dns durch tangentialfluss-filtration - Google Patents

Verfahren zur rnase- und organische lösemitteln- freier reinigung von plasmid-dns durch tangentialfluss-filtration

Info

Publication number
ATE264386T1
ATE264386T1 AT00948876T AT00948876T ATE264386T1 AT E264386 T1 ATE264386 T1 AT E264386T1 AT 00948876 T AT00948876 T AT 00948876T AT 00948876 T AT00948876 T AT 00948876T AT E264386 T1 ATE264386 T1 AT E264386T1
Authority
AT
Austria
Prior art keywords
plasmid dna
flow filtration
tangential flow
rnase
organic solvent
Prior art date
Application number
AT00948876T
Other languages
English (en)
Inventor
Michelle D Butler
Darien L Cohen
David Kahn
Marjorie E Winkler
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ATE264386T1 publication Critical patent/ATE264386T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/84—Manufacture, treatment, or detection of nanostructure
    • Y10S977/894—Manufacture, treatment, or detection of nanostructure having step or means utilizing biological growth
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/84—Manufacture, treatment, or detection of nanostructure
    • Y10S977/895—Manufacture, treatment, or detection of nanostructure having step or means utilizing chemical property
    • Y10S977/896—Chemical synthesis, e.g. chemical bonding or breaking
    • Y10S977/898—Enzymatic
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/908—Mechanical repair performed/surgical
    • Y10S977/91—Strengthening cell or tissue
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/908—Mechanical repair performed/surgical
    • Y10S977/912—Cancer cell repair
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/914—Protein engineering
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/924—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis using nanostructure as support of dna analysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
AT00948876T 1999-07-23 2000-07-21 Verfahren zur rnase- und organische lösemitteln- freier reinigung von plasmid-dns durch tangentialfluss-filtration ATE264386T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14531699P 1999-07-23 1999-07-23
US16520699P 1999-11-12 1999-11-12
PCT/US2000/019963 WO2001007599A1 (en) 1999-07-23 2000-07-21 Method for rnase- and organic solvent-free plasmid dna purification using tangential flow filtration

Publications (1)

Publication Number Publication Date
ATE264386T1 true ATE264386T1 (de) 2004-04-15

Family

ID=26842848

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00948876T ATE264386T1 (de) 1999-07-23 2000-07-21 Verfahren zur rnase- und organische lösemitteln- freier reinigung von plasmid-dns durch tangentialfluss-filtration

Country Status (18)

Country Link
US (1) US6313285B1 (de)
EP (1) EP1200578B1 (de)
JP (1) JP2003505077A (de)
KR (1) KR100669305B1 (de)
CN (1) CN1378592A (de)
AT (1) ATE264386T1 (de)
BR (1) BR0012627A (de)
CA (1) CA2378944A1 (de)
DE (1) DE60009912T2 (de)
DK (1) DK1200578T3 (de)
ES (1) ES2218185T3 (de)
HK (1) HK1048829A1 (de)
HU (1) HUP0202295A3 (de)
IL (1) IL147644A0 (de)
NZ (1) NZ516641A (de)
PL (1) PL364816A1 (de)
PT (1) PT1200578E (de)
WO (1) WO2001007599A1 (de)

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US6995246B1 (en) * 2000-10-19 2006-02-07 Akzo Nobel N.V. Methods for removing suspended particles from soluble protein solutions
GB0028361D0 (en) * 2000-11-21 2001-01-03 Glaxo Group Ltd Method of separating extra chromosomal dna from other cellular components
JP3547715B2 (ja) * 2001-03-06 2004-07-28 榮 新垣 ベクター精製用装置及び方法
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
EP1476573B1 (de) 2002-01-28 2010-01-06 Ambion, Inc. Für den nachweis von nukleinsäuren kompetente biologische rohderivate
JP2005538717A (ja) * 2002-09-13 2005-12-22 ヴァレンティス，インコーポレイテッド 核酸のプレパラティブスケールの精製のための装置および方法
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
CN1283792C (zh) 2003-11-14 2006-11-08 余伟明 生物大分子组分相分离法
US7790039B2 (en) * 2003-11-24 2010-09-07 Northwest Biotherapeutics, Inc. Tangential flow filtration devices and methods for stem cell enrichment
SE0400490D0 (sv) 2004-02-26 2004-02-26 Amersham Biosciences Ab Plasmid purification
CA2595594C (en) * 2005-01-31 2012-05-01 Merck & Co., Inc. Upstream and a downstream purification process for large scale production of plasmid dna
US20080102493A1 (en) * 2006-06-29 2008-05-01 Millipore Corporation Isolation of RNA and DNA from a biological sample
US7964350B1 (en) * 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
US20080300397A1 (en) * 2007-05-31 2008-12-04 Ge Healthcare Uk Limited Modified spin column for simple and rapid plasmid dna extraction
EP2088196A1 (de) 2008-02-08 2009-08-12 Boehringer Ingelheim RCV GmbH & Co KG Verfahren und Vorrichtungen zur Herstellung von Biomolekülen
WO2009129524A2 (en) * 2008-04-18 2009-10-22 The General Hospital Corporation High-throughput plasmid dna preparation
TWI469992B (zh) * 2008-08-28 2015-01-21 Baxter Int 濃縮剪切靈敏生物聚合物的方法
EP2367954B1 (de) * 2008-12-19 2015-02-18 Life Technologies Corporation Proteinase k inhibitore, verfahren und zusammensetzungen davon
EP2506857B1 (de) 2009-12-01 2018-02-14 Translate Bio, Inc. Verabreichung von mrna zur vermehrung von proteinen und enzymen bei humangenetischen erkrankungen
WO2012135679A2 (en) 2011-04-01 2012-10-04 Emd Millipore Corporation Nanofiber containing composite structures
AU2012267531B2 (en) 2011-06-08 2017-06-22 Translate Bio, Inc. Lipid nanoparticle compositions and methods for mRNA delivery
US20150267192A1 (en) 2012-06-08 2015-09-24 Shire Human Genetic Therapies, Inc. Nuclease resistant polynucleotides and uses thereof
ES2981185T3 (es) * 2013-03-14 2024-10-07 Translate Bio Inc Métodos para la purificación de ARN mensajero
EA202190410A1 (ru) 2013-03-14 2022-03-31 Шир Хьюман Дженетик Терапис, Инк. КОМПОЗИЦИИ мРНК CFTR И СВЯЗАННЫЕ С НИМИ СПОСОБЫ И ВАРИАНТЫ ПРИМЕНЕНИЯ
CN103525866A (zh) * 2013-03-22 2014-01-22 人福医药集团股份公司 制备质粒的方法
EA034103B1 (ru) 2013-10-22 2019-12-27 Транслейт Био, Инк. СПОСОБ ЛЕЧЕНИЯ ФЕНИЛКЕТОНУРИИ С ПРИМЕНЕНИЕМ мРНК
CA2928188A1 (en) 2013-10-22 2015-04-30 Shire Human Genetic Therapies, Inc. Mrna therapy for argininosuccinate synthetase deficiency
DK178664B1 (en) * 2013-10-25 2016-10-24 Amphi Consult V Martin Hesselsøe Aps A system and a method for concentrating traces of tissue from aquatic organisms in a water sample and use thereof
NZ721261A (en) * 2013-12-18 2017-09-29 Asahi Chemical Ind Method for detecting coliform bacteria contained in milk
JP6571679B2 (ja) 2014-04-25 2019-09-04 トランスレイト バイオ， インコーポレイテッド メッセンジャーｒｎａの精製方法
EP3160612B1 (de) 2014-06-26 2023-09-13 EMD Millipore Corporation Flüssigkeitsfiltrationsvorrichtung mit erhöhter schmutzhaltekapazität
KR102206963B1 (ko) * 2015-04-17 2021-01-25 이엠디 밀리포어 코포레이션 접선방향 유동 여과 모드에서 작동되는 나노섬유 한외여과막을 사용하여 샘플에서 목적하는 생물학적 물질을 정제하는 방법
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PL4108769T3 (pl) * 2015-05-29 2024-02-05 CureVac Manufacturing GmbH Sposób wytwarzania i oczyszczania rna obejmujący co najmniej jeden etap filtracji o przepływie stycznym
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Also Published As

Publication number Publication date
JP2003505077A (ja) 2003-02-12
WO2001007599A1 (en) 2001-02-01
IL147644A0 (en) 2002-08-14
HK1048829A1 (zh) 2003-04-17
HUP0202295A3 (en) 2005-01-28
ES2218185T3 (es) 2004-11-16
CA2378944A1 (en) 2001-02-01
PT1200578E (pt) 2004-08-31
EP1200578A1 (de) 2002-05-02
PL364816A1 (en) 2004-12-13
NZ516641A (en) 2003-07-25
KR20020019123A (ko) 2002-03-09
HUP0202295A2 (en) 2002-10-28
CN1378592A (zh) 2002-11-06
KR100669305B1 (ko) 2007-01-17
DK1200578T3 (da) 2004-07-26
EP1200578B1 (de) 2004-04-14
US6313285B1 (en) 2001-11-06
DE60009912T2 (de) 2005-04-14
DE60009912D1 (de) 2004-05-19
BR0012627A (pt) 2002-04-09

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