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ATE185844T1 - Genexpressionuntersuchung durch aufzeigen von cdna 3'-restriktionsfragmenten - Google Patents

Genexpressionuntersuchung durch aufzeigen von cdna 3'-restriktionsfragmenten

Info

Publication number
ATE185844T1
ATE185844T1 AT96928025T AT96928025T ATE185844T1 AT E185844 T1 ATE185844 T1 AT E185844T1 AT 96928025 T AT96928025 T AT 96928025T AT 96928025 T AT96928025 T AT 96928025T AT E185844 T1 ATE185844 T1 AT E185844T1
Authority
AT
Austria
Prior art keywords
gene expression
restriction fragments
expression study
detecting cdna
cdna
Prior art date
Application number
AT96928025T
Other languages
English (en)
Inventor
Sherman Weissman
Yatindra Prashar
Original Assignee
Univ Yale
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Yale filed Critical Univ Yale
Application granted granted Critical
Publication of ATE185844T1 publication Critical patent/ATE185844T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT96928025T 1995-08-01 1996-07-30 Genexpressionuntersuchung durch aufzeigen von cdna 3'-restriktionsfragmenten ATE185844T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/510,032 US5712126A (en) 1995-08-01 1995-08-01 Analysis of gene expression by display of 3-end restriction fragments of CDNA

Publications (1)

Publication Number Publication Date
ATE185844T1 true ATE185844T1 (de) 1999-11-15

Family

ID=24029083

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96928025T ATE185844T1 (de) 1995-08-01 1996-07-30 Genexpressionuntersuchung durch aufzeigen von cdna 3'-restriktionsfragmenten

Country Status (9)

Country Link
US (2) US5712126A (de)
EP (2) EP0955379A3 (de)
JP (1) JPH10509329A (de)
AT (1) ATE185844T1 (de)
AU (1) AU692403B2 (de)
CA (1) CA2201468C (de)
DE (1) DE69604775T2 (de)
IL (1) IL120582A0 (de)
WO (1) WO1997005286A1 (de)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1382386A3 (de) * 1992-02-19 2004-12-01 The Public Health Research Institute Of The City Of New York, Inc. Neue Ordnungen für Oligonukleotide und ihr Nutzen zum Sortieren, Isolieren, Sequenzierung und Manipulieren von Nukleinsäuren
RU2111254C1 (ru) * 1994-07-11 1998-05-20 Институт молекулярной биологии им.В.А.Энгельгардта РАН СПОСОБ ДЕТЕКЦИИ ДИФФЕРЕНЦИАЛЬНО ЭКСПРЕССИРУЮЩИХСЯ МАТРИЧНЫХ РНК И КЛОНИРОВАНИЯ СООТВЕТСТВУЮЩИХ ИМ ФРАГМЕНТОВ кДНК
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
US5712126A (en) * 1995-08-01 1998-01-27 Yale University Analysis of gene expression by display of 3-end restriction fragments of CDNA
US6395887B1 (en) 1995-08-01 2002-05-28 Yale University Analysis of gene expression by display of 3'-end fragments of CDNAS
US5871697A (en) 1995-10-24 1999-02-16 Curagen Corporation Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
US5972693A (en) * 1995-10-24 1999-10-26 Curagen Corporation Apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
US6418382B2 (en) 1995-10-24 2002-07-09 Curagen Corporation Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
US6426183B1 (en) 1995-12-21 2002-07-30 Kenneth L. Beattie Oligonucleotide microarrays: direct covalent attachment to glass
WO1997029211A1 (en) 1996-02-09 1997-08-14 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services RESTRICTION DISPLAY (RD-PCR) OF DIFFERENTIALLY EXPRESSED mRNAs
US6090556A (en) * 1997-04-07 2000-07-18 Japan Science & Technology Corporation Method for quantitatively determining the expression of a gene
US6261770B1 (en) 1997-05-13 2001-07-17 Display Systems Biotech Aps Method to clone mRNAs
EP0981609B1 (de) * 1997-05-13 2001-09-19 Azign Bioscience A/S Eine methode zum klonieren von mrna und darstellung von differentiell exprimierten transcripten (dodet)
WO1999010536A1 (en) 1997-08-22 1999-03-04 Yale University A process to study changes in gene expression in granulocytic cells
US7031843B1 (en) 1997-09-23 2006-04-18 Gene Logic Inc. Computer methods and systems for displaying information relating to gene expression data
DE19806431C1 (de) * 1998-02-17 1999-10-14 Novartis Ag Neues Verfahren zur Identifikation und Charakterisierung von mRNA-Molekülen
CA2326827A1 (en) * 1998-05-05 1999-11-11 Gene Logic, Inc. A process to study changes in gene expression in t lymphocytes
DE19822287C2 (de) 1998-05-18 2003-04-24 Switch Biotech Ag Klonierungsvektor, seine Herstellung und Verwendung zur Analyse von mRNA Expressionsmuster
US6287825B1 (en) * 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US6235502B1 (en) * 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
US6194158B1 (en) 1998-11-12 2001-02-27 Nyxis Neurotherapies, Inc. Diagnostic assay for cancer
US7482443B2 (en) * 2000-03-09 2009-01-27 Genetag Technology, Inc. Systems and methods to quantify and amplify both signaling probes for cDNA chips and genes expression microarrays
WO2001038572A1 (fr) * 1999-11-19 2001-05-31 Takara Bio Inc. Methode d'amplification d'acides nucleiques
US6713258B2 (en) 2000-03-29 2004-03-30 Center For The Application Of Molecular Biology To International Agriculture (Cambia) Methods for genotyping by hybridization analysis
US6387624B1 (en) * 2000-04-14 2002-05-14 Incyte Pharmaceuticals, Inc. Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing
AU2001279704B2 (en) * 2000-06-30 2005-12-15 Syngenta Participations Ag Method for identification, separation and quantitative measurement of nucleic acid fragments
US7300751B2 (en) * 2000-06-30 2007-11-27 Syngenta Participations Ag Method for identification of genetic markers
EP1356118A2 (de) 2001-01-24 2003-10-29 Syngenta Participations AG Methode zur herstellung einer nicht-redundanten bibliothek
US20030165865A1 (en) * 2001-01-29 2003-09-04 Hinkel Christopher A. Methods of analysis of nucleic acids
US20030082584A1 (en) * 2001-06-29 2003-05-01 Liang Shi Enzymatic ligation-based identification of transcript expression
US20030170695A1 (en) * 2001-06-29 2003-09-11 Liang Shi Enzymatic ligation-based identification of nucleotide sequences
US20030175908A1 (en) * 2002-01-29 2003-09-18 Sten Linnarsson Methods and means for manipulating nucleic acid
US7205454B2 (en) * 2002-07-31 2007-04-17 Bayer Bioscience N.V. Corn root preferential promoters and uses thereof
EP1862473B1 (de) 2002-07-31 2009-05-06 Bayer BioScience N.V. Maiswurzel-spezifische Promotoren und ihre Verwendungen
JP2004113108A (ja) * 2002-09-26 2004-04-15 Aisin Seiki Co Ltd Ｄｎａ回収法
JPWO2004044243A1 (ja) * 2002-11-13 2006-03-09 株式会社日立ハイテクノロジーズ 核酸の増幅方法及びその装置
JP4311003B2 (ja) * 2002-12-02 2009-08-12 アイシン精機株式会社 原核生物の遺伝子発現解析方法
US7667097B2 (en) * 2004-04-14 2010-02-23 Bayer Bioscience N.V. Rice pollen-preferential promoters and uses thereof
EP1815016B1 (de) 2004-11-01 2012-06-27 George Mason University Zusammensetzungen und verfahren zur diagnose von kolonerkrankungen
US20070231823A1 (en) 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
JP2010514692A (ja) 2006-12-20 2010-05-06 バイエル ヘルスケア リミティド ライアビリティ カンパニー 癌の治療に有用なヒドロキシメチルフェニルピラゾリル尿素化合物
WO2009133466A2 (en) 2008-04-30 2009-11-05 Population Genetics Technologies Ltd. Asymmetric adapter library construction
US20100167952A1 (en) * 2008-11-06 2010-07-01 Thomas Albert Suppression of secondary capture in microarray assays
WO2010133972A1 (en) 2009-05-22 2010-11-25 Population Genetics Technologies Ltd Sorting asymmetrically tagged nucleic acids by selective primer extension
WO2011021102A2 (en) 2009-08-20 2011-02-24 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
WO2011053987A1 (en) * 2009-11-02 2011-05-05 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence selection and amplification
WO2011055232A2 (en) 2009-11-04 2011-05-12 Population Genetics Technologies Ltd. Base-by-base mutation screening
US20130053253A1 (en) 2010-02-22 2013-02-28 Population Genetics Technologies Ltd Region of Interest Extraction and Normalization Methods
CN103079567A (zh) 2010-04-17 2013-05-01 拜尔健康护理有限责任公司 用于疾病和病症的治疗和预防的氟取代的ω-羧基芳基二苯脲的合成代谢产物
WO2011161549A2 (en) 2010-06-24 2011-12-29 Population Genetics Technologies Ltd. Methods and compositions for polynucleotide library production, immortalization and region of interest extraction
ES2523140T3 (es) 2010-09-21 2014-11-21 Population Genetics Technologies Ltd. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
US10246741B2 (en) 2011-05-27 2019-04-02 Oxford Nanopore Technologies Ltd. Coupling method
GB2497838A (en) 2011-10-19 2013-06-26 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
GB2533882B (en) 2012-01-26 2016-10-12 Nugen Tech Inc Method of enriching and sequencing nucleic acids of interest using massively parallel sequencing
WO2013124743A1 (en) 2012-02-22 2013-08-29 Population Genetics Technologies Ltd. Compositions and methods for intramolecular nucleic acid rearrangement ii
WO2013128281A1 (en) 2012-02-28 2013-09-06 Population Genetics Technologies Ltd Method for attaching a counter sequence to a nucleic acid sample
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
CN105637097A (zh) 2013-08-05 2016-06-01 特韦斯特生物科学公司 从头合成的基因文库
US9546399B2 (en) 2013-11-13 2017-01-17 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CN106460061B (zh) 2014-04-04 2020-03-06 牛津纳米孔技术公司 在双链核酸分子的两端使用纳米孔和锚分子来表征所述双链核酸的方法
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US10513732B2 (en) * 2015-07-13 2019-12-24 New York University Sequencing methods and kits
CA2998169A1 (en) 2015-09-18 2017-03-23 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
EP3440205B1 (de) 2016-04-07 2021-05-26 The Board of Trustees of the Leland Stanford Junior University Nichtinvasive diagnose durch sequenzierung von 5-hydroxymethylierter zellfreier dna
US11384382B2 (en) 2016-04-14 2022-07-12 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
EP3443066B1 (de) 2016-04-14 2024-10-02 Guardant Health, Inc. Verfahren zum frühen nachweis von krebs
JP6871364B2 (ja) 2016-09-21 2021-05-12 ツイスト バイオサイエンス コーポレーション 核酸に基づくデータ保存
CN110892485B (zh) 2017-02-22 2024-03-22 特韦斯特生物科学公司 基于核酸的数据存储
AU2018284227B2 (en) 2017-06-12 2024-05-02 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
KR20200047706A (ko) 2017-09-11 2020-05-07 트위스트 바이오사이언스 코포레이션 Gpcr 결합 단백질 및 이의 합성 방법
JP7066840B2 (ja) 2017-10-20 2022-05-13 ツイスト バイオサイエンス コーポレーション ポリヌクレオチド合成のための加熱されたナノウェル
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
EP3735459A4 (de) 2018-01-04 2021-10-06 Twist Bioscience Corporation Digitale informationsspeicherung auf dna-basis
US11634748B2 (en) 2018-02-14 2023-04-25 Clearnote Health, Inc. Methods for the epigenetic analysis of DNA, particularly cell-free DNA
EP3814497A4 (de) 2018-05-18 2022-03-02 Twist Bioscience Corporation Polynukleotide, reagenzien und verfahren zur nukleinsäurehybridisierung
WO2019246625A1 (en) 2018-06-22 2019-12-26 Bluestar Genomics, Inc. Hydroxymethylation analysis of cell-free nucleic acid samples for assigning tissue of origin, and related methods of use
EP3853383A1 (de) 2018-09-19 2021-07-28 Bluestar Genomics, Inc. Zellfreie dna-hydroxymethylierungsprofile zur beurteilung von pankreas-läsionen
MX2021003847A (es) 2018-10-04 2021-05-27 Bluestar Genomics Inc Analisis simultaneo de proteinas, nucleosomas y acidos nucleicos libres de celula, basado en secuenciacion, de una muestra biologica individual.
WO2020176362A1 (en) * 2019-02-25 2020-09-03 Twist Bioscience Corporation Compositions and methods for next generation sequencing
WO2020176680A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993006239A1 (en) * 1991-09-24 1993-04-01 Keygene N.V. Selective restriction fragment amplification: a general method for dna fingerprinting
GB9214873D0 (en) * 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
US5459037A (en) * 1993-11-12 1995-10-17 The Scripps Research Institute Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations
DE19518505A1 (de) * 1995-05-19 1996-11-21 Max Planck Gesellschaft Verfahren zur Genexpressionsanalyse
US5712126A (en) * 1995-08-01 1998-01-27 Yale University Analysis of gene expression by display of 3-end restriction fragments of CDNA

Also Published As

Publication number Publication date
AU692403B2 (en) 1998-06-04
IL120582A0 (en) 1997-08-14
DE69604775D1 (de) 1999-11-25
US6010850A (en) 2000-01-04
CA2201468A1 (en) 1997-02-13
AU6763696A (en) 1997-02-26
EP0955379A3 (de) 2000-03-01
US5712126A (en) 1998-01-27
EP0797685B1 (de) 1999-10-20
EP0797685A4 (de) 1998-06-10
DE69604775T2 (de) 2000-05-18
EP0955379A2 (de) 1999-11-10
EP0797685A1 (de) 1997-10-01
CA2201468C (en) 2002-03-05
WO1997005286A1 (en) 1997-02-13
JPH10509329A (ja) 1998-09-14

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