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AR042552A1 - Promotor artificial para la expresion de secuencias de adn en celulas vegetales - Google Patents

Promotor artificial para la expresion de secuencias de adn en celulas vegetales

Info

Publication number
AR042552A1
AR042552A1 ARP030104738A ARP030104738A AR042552A1 AR 042552 A1 AR042552 A1 AR 042552A1 AR P030104738 A ARP030104738 A AR P030104738A AR P030104738 A ARP030104738 A AR P030104738A AR 042552 A1 AR042552 A1 AR 042552A1
Authority
AR
Argentina
Prior art keywords
promoter
expression
artificial
plant cells
exon
Prior art date
Application number
ARP030104738A
Other languages
English (en)
Inventor
Gonzalez Osmany Ramos
Sosa Guillermo Selman-Housein
Rodriguez Alberto Salazar
Remedios Daymi Abreu
Original Assignee
Ct Ingenieria Genetica Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40293792&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR042552(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Ct Ingenieria Genetica Biotech filed Critical Ct Ingenieria Genetica Biotech
Publication of AR042552A1 publication Critical patent/AR042552A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un promotor artificial caracterizado porque es una molécula quimérica de ADN recombinante que después de ser introducida en células de plantas de cualquier clase promueve altos niveles de expresión de cualquier molécula de ADN fusionada a su extremo 3'. Los elementos genéticos base de la molécula promotora que aquí se describe son: un núcleo promotor con una caja TATA consenso seguida de un región Exón/Intrón/Exón y de un elementos potenciador de la actividad traduccional construidos todos artificialmente. Elementos reguladores de la expresión transcripcional pueden ser insertados upstream del promotor que aquí se describe para conferir a la expresión capacidad de respuesta temporal, órgano, o tejido específica. Los elementos genéticos artificiales disenadas pueden ser funcionalmente insertados entre cualquier promotor activo en células de plantas y una secuencia cualquiera de ADN, para incrementar los niveles de transcripción/traducción de esta última. Reivindicación 1: Un promotor artificial porque es una molécula de ADN recombinante que promueve en células vegetales la expresión de cualquier secuencia de ADN fusionada a su extremo 3', y que se compone de: a) un elemento 5' regulador de la transcripción seguido de, b) un núcleo promotor artificial que comprende una caja TATA, una secuencia nucleotídica con un contenido de GC menor de 64% y un sitio de iniciación de la transcripción, que en su 3' se una a, c) una secuencia nucleotídica sintética y no traducible, conformada por un primer Exón quimérico, un Intrón artificial capaz de potenciar en células vegetales la expresión de los genes fusionados a él, y un segundo Exón quimérico con propiedades de la traducción de cualquier gen insertado a continuación del mismo.
ARP030104738A 2002-12-27 2003-12-19 Promotor artificial para la expresion de secuencias de adn en celulas vegetales AR042552A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU20020337 2002-12-27

Publications (1)

Publication Number Publication Date
AR042552A1 true AR042552A1 (es) 2005-06-22

Family

ID=40293792

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP030104738A AR042552A1 (es) 2002-12-27 2003-12-19 Promotor artificial para la expresion de secuencias de adn en celulas vegetales

Country Status (13)

Country Link
US (1) US20060277628A1 (es)
EP (1) EP1577395A1 (es)
JP (1) JP2006512067A (es)
KR (1) KR20050090411A (es)
CN (1) CN1738902A (es)
AR (1) AR042552A1 (es)
AU (1) AU2003291914A1 (es)
BR (1) BR0317765A (es)
CA (1) CA2518966A1 (es)
MX (1) MXPA05006983A (es)
RU (1) RU2326167C2 (es)
WO (1) WO2004058979A1 (es)
ZA (1) ZA200505067B (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008081478A2 (en) * 2007-01-05 2008-07-10 Metahelix Life Sciences Private Limited A chimeric promoter and a method thereof
JP5158639B2 (ja) * 2008-04-11 2013-03-06 独立行政法人農業生物資源研究所 植物の内胚乳に特異的に発現する遺伝子および該遺伝子のプロモーター、並びにそれらの利用
JP5594683B2 (ja) * 2010-03-19 2014-09-24 国立大学法人静岡大学 プロモーター
ES2637862T3 (es) 2011-03-25 2017-10-17 Monsanto Technology Llc Elementos reguladores de plantas y usos de los mismos
CA2895184C (en) * 2012-12-19 2021-11-23 Monsanto Technology Llc Plant regulatory elements and uses thereof
MX359956B (es) * 2013-03-15 2018-10-16 Bayer Cropscience Lp Promotores constitutivos de soja.
EP3092309B1 (en) * 2014-01-10 2021-02-24 Medicago Inc. Cpmv enhancer elements
JP2023015421A (ja) * 2020-01-10 2023-02-01 日本たばこ産業株式会社 エンハンサー

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA88319B (en) * 1987-02-06 1988-08-12 Lubrizol Enterprises, Inc. Ocs enhancer
AU7182791A (en) * 1990-01-05 1991-07-24 Cornell Research Foundation Inc. Rice actin gene and promoter
US5641876A (en) * 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
DE4222407C1 (de) * 1992-07-08 1993-10-07 Max Planck Gesellschaft Modulartiges Promotor-Konstrukt
US6072050A (en) * 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
DE69732702T2 (de) * 1996-06-11 2005-07-28 Pioneer Hi-Bred International, Inc. Ein synthetischer, pflanzlicher kernpromotor und stromaufwaerts gelegenes, regulatorisches element
WO1998010081A2 (en) * 1996-09-03 1998-03-12 Plant Genetic Systems, N.V. Improved barstar-gene
US6573438B1 (en) * 1997-03-14 2003-06-03 Syngenta Participations Ag Inbred Maize line 2044BT
US6346655B1 (en) * 1999-03-31 2002-02-12 Syngenta Participations Ag Trichothecne-Resistant transgenic plants

Also Published As

Publication number Publication date
MXPA05006983A (es) 2006-02-22
EP1577395A1 (en) 2005-09-21
RU2326167C2 (ru) 2008-06-10
BR0317765A (pt) 2005-11-22
KR20050090411A (ko) 2005-09-13
US20060277628A1 (en) 2006-12-07
RU2005123815A (ru) 2006-01-27
JP2006512067A (ja) 2006-04-13
CA2518966A1 (en) 2004-07-15
WO2004058979A1 (es) 2004-07-15
AU2003291914A1 (en) 2004-07-22
CN1738902A (zh) 2006-02-22
ZA200505067B (en) 2006-05-31

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