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MXPA05002934A - Composiciones, metodos y kits para deteccion de un antigeno en una celula y en una mezcla biologica. - Google Patents

Composiciones, metodos y kits para deteccion de un antigeno en una celula y en una mezcla biologica.

Info

Publication number
MXPA05002934A
MXPA05002934A MXPA05002934A MXPA05002934A MXPA05002934A MX PA05002934 A MXPA05002934 A MX PA05002934A MX PA05002934 A MXPA05002934 A MX PA05002934A MX PA05002934 A MXPA05002934 A MX PA05002934A MX PA05002934 A MXPA05002934 A MX PA05002934A
Authority
MX
Mexico
Prior art keywords
cell
detecting
antigen
phage
red blood
Prior art date
Application number
MXPA05002934A
Other languages
English (en)
Inventor
Donald L Siegel
Original Assignee
Univ Pennsylvania
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Pennsylvania filed Critical Univ Pennsylvania
Publication of MXPA05002934A publication Critical patent/MXPA05002934A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invencion se refiere a nuevos metodos para detectar un miembro de un par de enlace conocido en una muestra, incluyendo una celula, donde un miembro del par (llamado el "receptor") se expresa por un bacteriofago, el fago luego se usa para detectar la presencia del otro miembro del par (llamado el "ligando" u "objetivo"). Antes que detectar el enlace del fago usando tecnologia a base de anticuerpos, la presente invencion se refiere a la deteccion de acido nucleico asociado con el fago. En un aspecto, la invencion se refiere a la identificacion de una porcion que porta antigeno (por ejemplo, un antigeno de globulo rojo) de interes presente en una celula, por ejemplo, un globulo rojo, usando bacteriofago que exhibe anticuerpo, asi como la deteccion de auto- o aloanticuerpos de anti-globulos rojos y/o complemento en una muestra, usando bacteriofago que exhibe retroactivo de antiglobulina y la deteccion de un acido nucleico asociado con el fago.
MXPA05002934A 2002-09-18 2003-09-18 Composiciones, metodos y kits para deteccion de un antigeno en una celula y en una mezcla biologica. MXPA05002934A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41169302P 2002-09-18 2002-09-18
PCT/US2003/029231 WO2004027028A2 (en) 2002-09-18 2003-09-18 Compositions, methods and kits for detection of an antigen on a cell and in a biological mixture

Publications (1)

Publication Number Publication Date
MXPA05002934A true MXPA05002934A (es) 2006-02-24

Family

ID=32030711

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA05002934A MXPA05002934A (es) 2002-09-18 2003-09-18 Composiciones, metodos y kits para deteccion de un antigeno en una celula y en una mezcla biologica.

Country Status (11)

Country Link
US (2) US8617805B2 (es)
EP (1) EP1554401B1 (es)
JP (1) JP4601427B2 (es)
AT (1) ATE386111T1 (es)
AU (1) AU2003272491B2 (es)
CA (1) CA2499355C (es)
DE (1) DE60319121T2 (es)
ES (1) ES2301818T3 (es)
HK (1) HK1077851A1 (es)
MX (1) MXPA05002934A (es)
WO (1) WO2004027028A2 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA05002934A (es) * 2002-09-18 2006-02-24 Univ Pennsylvania Composiciones, metodos y kits para deteccion de un antigeno en una celula y en una mezcla biologica.
GB0304832D0 (en) * 2003-03-04 2003-04-09 Secr Defence Assay method
WO2007035633A2 (en) 2005-09-16 2007-03-29 President & Fellows Of Harvard College Screening assays and methods
US20070100557A1 (en) * 2005-10-24 2007-05-03 Yi Zhang Selection of genotyped transfusion donors by cross-matching to genotyped recipients
EP1963494B1 (en) * 2005-11-29 2014-10-08 The Trustees of The University of Pennsylvania Phage particle diagnostic reagents
PL2036989T3 (pl) * 2007-09-12 2012-12-31 Pasteur Institut Polinukleotyd odpowiedni do testu reporterowego dla pojedynczych komórek do monitorowania schematów ekspresji genów z dużą rozdzielczością przestrzenną i czasową
CN102944684B (zh) * 2012-11-07 2014-09-10 武汉康莱博生物科技有限公司 抗a、抗b血型定型试剂
US20200333322A1 (en) * 2017-07-31 2020-10-22 Essenlix Corporation Assays with reduced interference
WO2019069980A1 (ja) * 2017-10-04 2019-04-11 晋一郎 川本 大腸癌の検出方法
WO2019152878A1 (en) * 2018-02-02 2019-08-08 Bloodworks Antibody screens using transgenic antigen(s)
GB201906331D0 (en) * 2019-05-03 2019-06-19 Governing Council Of The Univ Of Toronto Digital microfluidic agglutination assays

Family Cites Families (19)

* Cited by examiner, † Cited by third party
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US5856092A (en) * 1989-02-13 1999-01-05 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
US5698426A (en) 1990-09-28 1997-12-16 Ixsys, Incorporated Surface expression libraries of heteromeric receptors
WO1992018619A1 (en) * 1991-04-10 1992-10-29 The Scripps Research Institute Heterodimeric receptor libraries using phagemids
ATE210189T1 (de) * 1992-09-04 2001-12-15 Scripps Research Inst Phagemiden die einen oberflächenrezeptor und ein heterologes oberflächenprotein co-exprimieren
DK0744958T3 (da) * 1994-01-31 2003-10-20 Univ Boston Polyklonale antistofbiblioteker
US5691132A (en) * 1994-11-14 1997-11-25 Cerus Corporation Method for inactivating pathogens in red cell compositions using quinacrine mustard
AU707484B2 (en) * 1995-09-14 1999-07-08 Regents Of The University Of California, The Antibodies specific for native PrPsc
JP2744938B2 (ja) * 1996-01-30 1998-04-28 工業技術院長 多孔質高強度低熱伝導窒化ケイ素質セラミックス及びその製造方法
US5716784A (en) * 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
US6747136B2 (en) * 1996-04-19 2004-06-08 Karolinska Innovations Ab Human monoclonal antibodies specific for hepatitis C virus (HCV) E2 antigen
ATE428801T1 (de) * 1996-06-04 2009-05-15 Univ Utah Res Found Überwachung der hybridisierung während pcr
US5985543A (en) * 1996-10-11 1999-11-16 The Trustees Of The University Of Pennsylvania Compositions and methods for detection of antibody binding to cells
US6255455B1 (en) * 1996-10-11 2001-07-03 The Trustees Of The University Of Pennsylvania Rh(D)-binding proteins and magnetically activated cell sorting method for production thereof
US5876925A (en) * 1996-10-11 1999-03-02 The Trustees Of The University Of Pennsylvania Magnetically activated cell sorting for production of proteins
US6153411A (en) * 1998-10-30 2000-11-28 American Water Works Company, Inc. Methods and kits for detection of Cryptosporidium parvum using immunomagnetic separation and amplification
AU778776B2 (en) * 1999-04-14 2004-12-23 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The High sensitivity phage display biomolecule detection
US6615063B1 (en) * 2000-11-27 2003-09-02 The General Hospital Corporation Fluorescence-mediated molecular tomography
MXPA05002934A (es) * 2002-09-18 2006-02-24 Univ Pennsylvania Composiciones, metodos y kits para deteccion de un antigeno en una celula y en una mezcla biologica.
EP1963494B1 (en) * 2005-11-29 2014-10-08 The Trustees of The University of Pennsylvania Phage particle diagnostic reagents

Also Published As

Publication number Publication date
US8617805B2 (en) 2013-12-31
JP2006516088A (ja) 2006-06-22
US20050136399A1 (en) 2005-06-23
DE60319121T2 (de) 2009-02-05
HK1077851A1 (en) 2006-02-24
AU2003272491B2 (en) 2008-10-02
CA2499355C (en) 2012-07-17
EP1554401A4 (en) 2006-04-12
EP1554401A2 (en) 2005-07-20
ATE386111T1 (de) 2008-03-15
US20050191622A1 (en) 2005-09-01
EP1554401B1 (en) 2008-02-13
WO2004027028A3 (en) 2005-05-26
DE60319121D1 (de) 2008-03-27
ES2301818T3 (es) 2008-07-01
AU2003272491A1 (en) 2004-04-08
WO2004027028A2 (en) 2004-04-01
JP4601427B2 (ja) 2010-12-22
US8586293B2 (en) 2013-11-19
CA2499355A1 (en) 2004-04-01

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