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CL2016003247A1 - Composiciones y métodos para la expresión de arns guía usando el promotor h1 - Google Patents

Composiciones y métodos para la expresión de arns guía usando el promotor h1

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Publication number
CL2016003247A1
CL2016003247A1 CL2016003247A CL2016003247A CL2016003247A1 CL 2016003247 A1 CL2016003247 A1 CL 2016003247A1 CL 2016003247 A CL2016003247 A CL 2016003247A CL 2016003247 A CL2016003247 A CL 2016003247A CL 2016003247 A1 CL2016003247 A1 CL 2016003247A1
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CL
Chile
Prior art keywords
expression
promoter
methods
compositions
arng
Prior art date
Application number
CL2016003247A
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English (en)
Inventor
Donald Zack
Ranga Vinod Jaskula
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Univ Johns Hopkins
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Application filed by Univ Johns Hopkins filed Critical Univ Johns Hopkins
Publication of CL2016003247A1 publication Critical patent/CL2016003247A1/es

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Abstract

<p>LA MATERIA OBJETO DE LA PRESENTE DIVULGACIÓN PROPORCIONA COMPOSICIONES Y MÉTODOS PARA LA EXPRESIÓN DE ARNs GUÍAS DE CRISPR UTILIZANDO EL PROMOTOR H1. EN PARTICULAR, SE PROPORCIONAN COMPOSICIONES Y MÉTODOS PARA EL USO DEL PROMOTOR H1 PARA EXPRESAR UN ARN GUÍA (ARNg) DE CRISPR CON ESPECIFICIDAD ALTERADA DEL NUCLEÓTIDO EN 5', ASÍ COMO EL USO DE LA SECUENCIA PROMOTORA H1 COMO UN PROMOTOR BIDIRECCIONAL PARA EXPRESAR LA NUCLEASA Cas9 Y EL ARNg SIMULTÁNEAMENTE. TAMBIÉN SE PROPORCIONAN COMPOSICIONES Y MÉTODOS PARA LA EXPRESIÓN Y LA REGULACIÓN DE LA EXPRESIÓN ARNg IN VIVO MEDIANTE EL USO DE RIBOZIMAS DE ARN Y APTAZIMAS REGULABLES.</p>
CL2016003247A 2014-06-16 2016-12-16 Composiciones y métodos para la expresión de arns guía usando el promotor h1 CL2016003247A1 (es)

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US201462012802P 2014-06-16 2014-06-16

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EP (2) EP3155101B1 (es)
JP (2) JP6930834B2 (es)
KR (1) KR102649341B1 (es)
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AU (2) AU2015277369B2 (es)
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