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24 pages, 4479 KiB  
Article
Assessing the Wind Energy Potential: A Case Study in Fort Hare, South Africa, Using Six Statistical Distribution Models
by Ngwarai Shambira, Patrick Mukumba and Golden Makaka
Appl. Sci. 2025, 15(5), 2778; https://doi.org/10.3390/app15052778 (registering DOI) - 5 Mar 2025
Abstract
Wind energy is a clean, inexhaustible resource with significant potential to reduce coal dependence, lower carbon emissions, and provide sustainable energy in the off-grid areas of South Africa’s Eastern Cape. However, due to wind variability, site-specific assessments are crucial for accurate resource estimation [...] Read more.
Wind energy is a clean, inexhaustible resource with significant potential to reduce coal dependence, lower carbon emissions, and provide sustainable energy in the off-grid areas of South Africa’s Eastern Cape. However, due to wind variability, site-specific assessments are crucial for accurate resource estimation and investment risk mitigation. This study evaluates the wind energy potential at Fort Hare using six statistical distribution models: Weibull (WEI), Rayleigh (RAY), gamma (GAM), generalized extreme value (GEV), inverse Gaussian (IGA), and Gumbel (GUM). The analysis is based on three years (2021–2023) of hourly wind speed data at 10 m above ground level from the Fort Beaufort weather station. Parameters were estimated using the maximum likelihood method (MLM), and model performance was ranked using the total error (TE) metric. The results indicate an average wind speed of 2.60 m/s with a standard deviation of 1.85 m/s. The GEV distribution was the best fit (TE = 0.020), while the widely used Weibull distribution ranked third (TE = 0.5421), highlighting its limitations in capturing wind variability and extremes. This study underscores the importance of testing multiple models for accurate wind characterization and suggests improving the performance of the Weibull model through advanced parameter optimization, such as artificial intelligence. The wind power density was 31.52 W/m2, classifying the site as poor for large-scale electricity generation. The prevailing wind direction was southeast. Recommendations include deploying small-scale turbines and exploring augmentative systems to optimize wind energy utilization in the region. Full article
(This article belongs to the Special Issue Advances and Challenges in Wind Turbine Mechanics)
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<p>Wind farms in South Africa and their capacity (MW) [<a href="#B11-applsci-15-02778" class="html-bibr">11</a>].</p>
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<p>Location of study area.</p>
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<p>Monthly average wind speed at 10m AGL for the University of Fort Hare.</p>
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<p>Comparison of statistical metrics for the six distributions.</p>
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<p>Diurnal variation in mean wind speed at the University of Fort Hare.</p>
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<p>Weibull distributions fitted to the observed data histogram for 2021–2023.</p>
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<p>Histograms with fitted distributions for seasonal wind speed data.</p>
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<p>Contributions of normalized KSS, AD, and WPDE to TE for each distribution in <a href="#applsci-15-02778-t013" class="html-table">Table 13</a>.</p>
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<p>Wind rose diagram for overall wind direction for the 2021–2023 period.</p>
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<p>Wind rose diagram for seasonal wind direction variations for the 2021–2023 period.</p>
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20 pages, 359 KiB  
Article
The Impact of Audit Quality and Female Audit Committee Characteristics on Earnings Management: Evidence from the UK
by Naila Amara, Saad Bourouis, Sajead Mowafaq Alshdaifat, Houssam Bouzgarrou and Hamzeh Al Amosh
J. Risk Financial Manag. 2025, 18(3), 136; https://doi.org/10.3390/jrfm18030136 (registering DOI) - 5 Mar 2025
Abstract
This study explores the impact of audit quality and the proportion of women on an audit committee on earnings management. Moreover, we examined how age diversity and the presence of non-foreign women on audit committees influence earnings management. Our study utilizes data from [...] Read more.
This study explores the impact of audit quality and the proportion of women on an audit committee on earnings management. Moreover, we examined how age diversity and the presence of non-foreign women on audit committees influence earnings management. Our study utilizes data from 165 UK-based listed companies between 2011 and 2021. A combination of static and dynamic analysis was used to empirically reveal our results. The results show a negative and significant relationship between audit quality and earnings management, as per the Kothari model. The presence of a female audit committee does not affect earnings management. However, when we control for demographic variables like age and nationality, we found that non-foreign female members of the audit committee reduced earnings management, while age diversity among female members had no effect. Additional analysis using the Dechow model revealed that both the presence of a female audit committee and their nationality affected earnings management. Our findings contribute to ongoing discussions on corporate governance by providing evidence that female audit committees and audit quality influence earnings management in UK-listed companies. This study is one of the few that examines demographic attributes (e.g., nationality or age). Full article
(This article belongs to the Special Issue Corporate Governance and Earnings Management)
21 pages, 6223 KiB  
Article
Preliminary Analysis of Quantum Dots as a Marking Technique for Ceratitis capitata
by Richard Wimbush, Pia Addison, Francois Bekker, Minette Karsten, Melissa Powell, George Marais, Aaisha Moerat, Anandie Bierman and John S. Terblanche
Insects 2025, 16(3), 270; https://doi.org/10.3390/insects16030270 (registering DOI) - 4 Mar 2025
Abstract
This study evaluates the potential of quantum dots (QDs) as a marking method for Mediterranean fruit flies (Ceratitis capitata) (Medfly) in comparison to traditional fluorescent powder. As a highly destructive pest impacting a wide variety of fruit crops, an effective marking [...] Read more.
This study evaluates the potential of quantum dots (QDs) as a marking method for Mediterranean fruit flies (Ceratitis capitata) (Medfly) in comparison to traditional fluorescent powder. As a highly destructive pest impacting a wide variety of fruit crops, an effective marking technique is essential for improving the biological understanding and management of Medflies, including control strategies like the Sterile Insect Technique (SIT). Through multiple controlled experiments, we examined the effects of QDs and fluorescent powder markers on Medfly flight ability, marker retention rates, and marker durability and stability under diverse storage conditions. Fluorescent powder demonstrated consistently high reliability across all parameters, whereas QDs showed reduced retention, particularly when applied to pupae, and had a more pronounced negative effect on flight ability. This was illustrated by the field trials, which did not recapture any of the QD-marked flies, highlighting the current limitations in QD application methods. Additionally, fluorescent powders outperformed QDs in both long-term storage conditions and short-term stability tests. These findings indicate that while QDs possess potential as marking agents, further refinement of application techniques is required to achieve comparable efficacy to fluorescent powders in pest management contexts. Full article
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<p>(<b>A</b>) The percentage of Medflies marked after 8 days using three marking techniques: QDs applied during the adult stage (QA), QDs applied during the pupal stage (QP), and fluorescent powder applied during the pupal stage (P). A total of 240 Medflies (N = 240) were used across the experiment, with each treatment group consisting of 20 specimens per replicate, repeated four times. Vertical bars indicate 95% confidence intervals. Significant differences between treatments are denoted by different letters (a, b, c), where <span class="html-italic">p</span> &lt; 0.01, indicating that marking retention varied significantly between the groups. (<b>B</b>) Comparison of the marking percentages across sexes (male and female) for the different marking treatments. The same three marking techniques were evaluated, with males and females analyzed separately to assess potential sex-based differences in marking retention. Vertical bars represent 95% confidence intervals.</p>
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<p>The total number of Medflies able to fly under different marking treatments when attracted by a food and light source. Medflies were marked using one of two techniques: QDs applied during the adult stage (QD) or fluorescent powder applied during the pupal stage (FP). A control group (C) of unmarked Medflies was also included for comparison. A total of 240 Medflies (N = 240) were tested in a controlled flight trial, with each treatment group consisting of 20 specimens per replicate, repeated four times. Vertical bars indicate the number of observations, with the percentage of individuals from each marking treatment that could fly displayed on each bar.</p>
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<p>The total number of Medflies combined at 24, 48, and 72 h, categorized as flyers, non-flyers, or those that did not emerge during the mark–release–recapture study. Medflies were marked with either fluorescent powder (<b>A</b>), yellow QDs (<b>B</b>), red QDs (<b>C</b>), or orange QDs (<b>D</b>) during the pupal stage to assess the persistence and effectiveness of QD markings in comparison to fluorescent powder. A total of 12,000 Medflies were marked and released (N = 12,000), with equal distribution across all marking groups (3000 Medflies per group, repeated across three experimental replicates). Bars indicate the total number of observations, with percentage breakdown of the different categories displayed on each bar. Environmental conditions during the study varied across replicates, with temperatures ranging from 15.8 °C to 19.2 °C. The experiment was conducted over two seasons (Autumn and Spring), allowing for assessment under different climatic conditions.</p>
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<p>The percentage of Medflies that remained successfully marked with QDs during the six storage treatments (T1–T6) across all time intervals (2, 4, and 8 weeks). The treatments represent different storage and handling conditions, including storage in ethanol at different temperatures (−20 °C (T1), −80 °C (T2), and room temperature(T3)), dry storage at room temperature (T4), and rinsing in water (T5) or ethanol (T6) before dry storage. This experiment aimed to assess the retention and persistence of QD markings on Medflies under different storage conditions, compared to traditional fluorescent powders. The fluorescent powder retained 100% of its markings across all treatments and time intervals, and was therefore not included in the graph. For the entire experimental study, a total of 2160 Medflies were used, evenly distributed across all six treatments and time periods (N = 2160). Vertical bars indicate 95% confidence intervals. Retention of markings was evaluated under UV light, with flies examined at each time point to determine the stability of QDs and fluorescent powders over time.</p>
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<p>Visualization of fluorescent powder marking on Medflies under different lighting conditions. The left image shows a marked fly under normal light and the right image shows the same marking under UV illumination.</p>
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<p>Visualization of QD fluorescence in marked Medflies under UV light using a stereomicroscope. (<b>A</b>) displays all three QD colors—orange, yellow, and red—side by side for comparison. (<b>B</b>–<b>D</b>) show the individual fluorescence of orange, yellow, and red QDs, respectively. These images are intended to illustrate the emission properties of each QD color under UV illumination and do not represent the actual quantity used in experimental applications. While red and orange may appear visually similar in the images, their differences are more distinct when observed directly.</p>
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<p>Fluorescence of Mediterranean fruit flies (<span class="html-italic">Ceratitis capitata</span>) marked with orange quantum dots (QDs) under UV light. These adult Medflies were marked with QDs as part of the “suitable marking technique” experiment and imaged using a stereomicroscope under UV illumination. The fluorescence observed indicates the retention and distribution of QDs on the insect exoskeleton post-marking.</p>
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12 pages, 1758 KiB  
Article
The Russia–Ukraine Conflict: A Global Impact Assessment in the Corn and Wheat Sectors
by Nkongho Ayuketang Arreyndip
Agriculture 2025, 15(5), 550; https://doi.org/10.3390/agriculture15050550 - 4 Mar 2025
Abstract
According to data from the Food and Agricultural Organization of the United Nations (FAO), Ukraine was the fifth and ninth global producer and exporter of corn and wheat, respectively, before Russia’s invasion. The disruption of the supply chain in Ukraine in these food [...] Read more.
According to data from the Food and Agricultural Organization of the United Nations (FAO), Ukraine was the fifth and ninth global producer and exporter of corn and wheat, respectively, before Russia’s invasion. The disruption of the supply chain in Ukraine in these food sectors due to the Russian–Ukrainian conflict can hamper global food security. Very little is known about how the war has impacted these important food sectors globally. This paper examines the global impact of the war on the corn and wheat sectors in the first year of the war and investigates the relationship between market vulnerability and trade ties with the conflicting regions. Analysis of FAO data shows that Ukraine suffered a 12.87% and 17.45% production decline in the corn and wheat sectors, respectively, compared to the 2012–2021 base years. Using the Acclimate economic network model, these shocks are applied to Ukraine’s corn and wheat network nodes to analyze their global impact. The production value and total losses are calculated and compared to the base year, both regionally and in some major global economic blocs. The results show that the corn sectors in Germany, Ukraine, Poland, and Belgium suffered the largest production value losses, while Ukraine, China, Venezuela, and Korea suffered the largest overall losses. In the wheat sector, Russia, Germany, Ukraine, and Canada suffered the largest production value losses, while Ukraine, Kazakhstan, Uzbekistan, and China suffered the largest overall losses. Overall, the corn sector was the hardest hit globally compared to the wheat sector, with the EU, the US, China, South America, and Africa being the hardest hit in the corn sector, while BRICS and Oceania were the hardest hit in the wheat sector. The study equally finds a strong correlation between increased regional food market vulnerability and Ukraine’s trade relations. These findings can contribute to better investment decisions, regional and sectoral emergency management planning, and the development of regulatory frameworks. Full article
(This article belongs to the Section Agricultural Economics, Policies and Rural Management)
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<p>The figure shows Ukraine’s cereal trading partners (from 2011 to 2021), with countries such as China, Egypt, Indonesia, Spain, the Netherlands, Turkey, and Tunisia being the main trading partners. The data were taken from the World Integrated Trade Solutions (WITS) found at <a href="https://wits.worldbank.org/" target="_blank">https://wits.worldbank.org/</a> (accessed on 13 December 2024).</p>
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<p>(<b>a</b>) A global share of corn and wheat production averaged from 2011 to 2022. (<b>b</b>,<b>c</b>) Ukraine’s and Russia’s corn and wheat production time series, respectively, over the study period. (<b>d</b>) The changes in Ukraine’s corn and wheat production with reference to the 2012–2021 baseline. (<b>e</b>,<b>f</b>) The top 10 corn- and wheat-producing regions, respectively. (<b>g</b>) The top 10 most produced items in Ukraine. This figure shows Ukraine as a global producer of corn and wheat, corn and wheat being among the top three of Ukraine’s most produced items and Ukraine being the only region that was impacted by their war with Russia as per the time series. The data were taken from the Food and Agricultural Organization of the United Nations (FAO) found at <a href="https://www.fao.org/faostat/en/#data/QCL" target="_blank">https://www.fao.org/faostat/en/#data/QCL</a> (accessed on 1 December 2024).</p>
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<p>Global change in corn production value and total losses compared to the 2012–2022 baseline. The top 20 most and least impacted regions. Negative production values (red) are regions most impacted, while positive production values (blue) are regions least impacted. For total losses, negative losses (red) are regions least impacted, while positive losses (blue) are regions most impacted. Germany, Ukraine, Poland, and Belgium suffered the most production value losses, while China, the USA, the Netherlands, and Argentina were the least affected. For total losses, Ukraine, China, Venezuela, and Korea suffered the most total losses, while Germany, Poland, the USA, and Belgium suffered the least total losses. The maps were generated using the Datawrapper online tool found at <a href="https://www.datawrapper.de/" target="_blank">https://www.datawrapper.de/</a> (accessed on 27 December 2024).</p>
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<p>Global change in wheat production value and total losses compared to the 2012–2022 baseline. The top 20 most and least impacted regions. Negative production values (red) are regions most impacted, while positive production values (blue) are regions least impacted. For total losses, negative losses (red) are regions least impacted, while positive losses (blue) are regions most impacted. Russia, Germany, Ukraine, and Canada suffered the most production value losses, while China, Kazakhstan, France, and Uzbekistan were the least affected. For total losses, Ukraine, Kazakhstan, Uzbekistan, and China suffered the most total losses, while Russia, Germany, the Netherlands, and France suffered the least total losses. The maps were generated using the Datawrapper online tool found at <a href="https://www.datawrapper.de/" target="_blank">https://www.datawrapper.de/</a> (accessed on 27 December 2024).</p>
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<p>Shocks on regional economic blocs during the first year of hostilities. The maize sector is shown to be the hardest hit globally compared to the wheat sector, with the EU, US, China, South America, and Africa being the hardest hit in the maize sector, while BRICS and Oceania are the hardest hit in the wheat sector. Looking at the total losses, China shows economic recovery in the corn and wheat sectors, followed by a moderate recovery in South America, the EU, Africa, and BRICS states.</p>
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<p>Continental impacts during the first year of hostilities. Plots show Europe suffered the most total losses in the corn and wheat sectors and the most drop in corn production value, while Asia experienced a significant drop in wheat production value. North America is shown to be the least impacted compared to all continents.</p>
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21 pages, 3502 KiB  
Article
Exploring the Inhibitory Potential of Sodium Alginate Against Digestive Enzymes Linked to Obesity and Type 2 Diabetes
by Chantal D. Daub, Arryn L. Michaels, Blessing Mabate, Lithalethu Mkabayi, Adrienne L. Edkins and Brett I. Pletschke
Molecules 2025, 30(5), 1155; https://doi.org/10.3390/molecules30051155 - 4 Mar 2025
Abstract
Obesity and type 2 diabetes mellitus (T2DM) are major health concerns worldwide, often managed with treatments that have significant limitations and side effects. This study examines the potential of sodium alginates, extracted from Ecklonia radiata and Sargassum elegans, to inhibit digestive enzymes [...] Read more.
Obesity and type 2 diabetes mellitus (T2DM) are major health concerns worldwide, often managed with treatments that have significant limitations and side effects. This study examines the potential of sodium alginates, extracted from Ecklonia radiata and Sargassum elegans, to inhibit digestive enzymes involved in managing these conditions. We chemically characterized the sodium alginates and confirmed their structural integrity using FTIR, NMR, and TGA. The focus was on evaluating their ability to inhibit key digestive enzymes relevant to T2DM (α-amylase, α-glucosidase, sucrase, maltase) and obesity (pancreatic lipase). Enzyme inhibition assays revealed that these sodium alginates moderately inhibit α-glucosidase, maltase, and lipase by up to 43%, while showing limited effects on sucrase and α-amylase. In addition, the sodium alginates did not affect glucose uptake in human colorectal cells (HCT116), indicating they do not impact cellular glucose absorption. In summary, while the observed enzyme inhibition was moderate, the targeted inhibition of α-glucosidase, maltase, and lipase suggests that sodium alginates could be beneficial for managing postprandial hyperglycemia and lipid absorption in the context of T2DM and obesity. Full article
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<p>Thermogravimetric (TGA) (<b>A</b>) and derivative thermogravimetric (DTG) (<b>B</b>) curves of the sodium alginates. The thermal profiles highlight the distinct degradation stages and mass loss patterns, comparing <span class="html-italic">E. radiata</span>, <span class="html-italic">S. elegans</span>, and commercial sodium alginate samples.</p>
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<p>FTIR spectra of sodium alginates extracted from the brown seaweeds <span class="html-italic">E. radiata</span> and <span class="html-italic">S. elegans</span>, compared to the commercial sodium alginate standard.</p>
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<p><sup>1</sup>H NMR spectra at 70 °C of commercial sodium alginate and alginate extracted from <span class="html-italic">E. radiata</span> and <span class="html-italic">S. elegans</span> harvested from the South African coastline. The underlined M and G denote proton signals corresponding to D-mannuronic acid (M) and L-guluronic acid (G) residues, respectively. Non-underlined letters represent neighboring residues along the copolymer chain. The numbers indicate the specific protons responsible for each signal in the spectra.</p>
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<p>Pancreatic lipase inhibition by acid-extracted sodium alginates from brown seaweeds. (<b>A</b>) Inhibition activity against lipase using <span class="html-italic">p</span>-nitrophenyl butyrate as a substrate. (<b>B</b>) Inhibition activity against lipase using <span class="html-italic">p</span>-nitrophenyl octanoate as a substrate. Data represent the percentage inhibition of lipase activity by the extracted alginates compared to a control. Error bars indicate the standard deviation (<span class="html-italic">n</span> = 3). Statistical significance of inhibition level was assessed using two-way ANOVA, significance was defined as <span class="html-italic">p</span> value &lt; 0.0001 (****), being more significant than <span class="html-italic">p</span> value &gt; 0.0002 (***), 0.0021 (**) and 0.0332 (*), respectively.</p>
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<p>Mode of inhibition studies on pancreatic lipase. The effect of extracted sodium alginates on <span class="html-italic">p</span>-nitrophenyl butyrate and octanoate is shown. (<b>A</b>) <span class="html-italic">E. radiata</span> on <span class="html-italic">p</span>-nitrophenyl butyrate. (<b>B</b>) <span class="html-italic">S. elegans</span> on <span class="html-italic">p</span>-nitrophenyl butyrate. (<b>C</b>) <span class="html-italic">E. radiata</span> on <span class="html-italic">p</span>-nitrophenyl octanoate. (<b>D</b>) <span class="html-italic">S. elegans</span> on <span class="html-italic">p</span>-nitrophenyl octanoate. (X<sub>1</sub>) respective Michaelis–Menten plot. (X<sub>2</sub>) respective Lineweaver–Burk plot. Error bars indicate standard deviations (<span class="html-italic">n</span> = 3).</p>
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<p>Inhibition of type 2 diabetes-relevant enzymes: (<b>A</b>) α-amylase, (<b>B</b>) α-glucosidase, (<b>C</b>) maltase, and (<b>D</b>) sucrase by sodium alginates and acarbose (positive control). Error bars indicate the standard deviation (<span class="html-italic">n</span> = 6).</p>
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<p>(<b>A</b>,<b>B</b>) Fluorescent emission spectra and (<b>C</b>,<b>D</b>) modified Stern–Volmer plots for fluorescent quenching of α-glucosidase by sodium alginate from <span class="html-italic">E. radiata</span> and <span class="html-italic">S. elegans</span>.</p>
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<p>Glucose uptake in HCT116 cells treated with 2-NBDG under various conditions after 5 h incubation. Fluorescence intensity per mg protein is presented as mean ± SD (<span class="html-italic">n</span> = 3), with normalization for cell number based on protein content determined by the BCA (Bicinchoninic acid) protein assay.</p>
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30 pages, 8318 KiB  
Article
Timing and Duration of Drought Differentially Affect Growth and Yield Components Among Sugarcane Genotypes
by Amarawan Tippayawat, Sanun Jogloy, Nimitr Vorasoot, Nakorn Jongrungklang, Collins A. Kimbeng, John L. Jifon, Jidapa Khonghintaisong and Patcharin Songsri
Plants 2025, 14(5), 796; https://doi.org/10.3390/plants14050796 - 4 Mar 2025
Abstract
Drought significantly impacts sugarcane yield, making drought resistance an important trait in drought-prone regions. The effects of the timing and duration of drought on yield and yield components, including relationships among these traits, were examined using a diverse set of sugarcane genotypes in [...] Read more.
Drought significantly impacts sugarcane yield, making drought resistance an important trait in drought-prone regions. The effects of the timing and duration of drought on yield and yield components, including relationships among these traits, were examined using a diverse set of sugarcane genotypes in a 2-year (planted cane and first ratoon) field study. Three drought treatments (no water stress (SD0), short-term (SD1), and long-term (SD2) drought) were assigned as the main plot and replicated four times. Within each plot, six genotypes were nested in a split-plot design. Drought reduced yield and its components, with the decline greater in SD2 than in SD1. Strong relationships between yield and its components like stalk height and density and height growth rate, especially under drought, make these traits potential surrogates for yield in drought screening experiments. The genotypes F03–362 and KK3 displayed high, stable yield potential across drought treatments, but KK3 lost potential in ratoon crop under drought. Although KK09–0358 displayed high yield potential, it was very sensitive to drought stress while UT12 and KK09–0939 displayed low yield potential and sensitivity to drought. TPJ04–768 displayed low but stable yield potential across drought treatments and crops. F03–362 and TPJ04–768 have utility in studies seeking to couple physiological with agronomic parameters promoting drought resistance and as parents for developing cultivars combining high and stable yield performance under drought. Full article
(This article belongs to the Special Issue Mechanisms of Plant Defense Against Abiotic Stresses)
24 pages, 4006 KiB  
Review
Molecular Epidemiology of Streptococcus pneumoniae Serotype 1: A Systematic Review of Circulating Clones and Clonal Clusters
by Onyansaniba K. Ntim and Eric S. Donkor
Int. J. Mol. Sci. 2025, 26(5), 2266; https://doi.org/10.3390/ijms26052266 - 4 Mar 2025
Viewed by 48
Abstract
Streptococcus pneumoniae serotype 1 is one of the most prevalent serotypes commonly associated with invasive pneumococcal disease cases and outbreaks worldwide. Several sequence types of this serotype have been identified globally, including those exhibiting both high virulence potential and antimicrobial resistance profiles. This [...] Read more.
Streptococcus pneumoniae serotype 1 is one of the most prevalent serotypes commonly associated with invasive pneumococcal disease cases and outbreaks worldwide. Several sequence types of this serotype have been identified globally, including those exhibiting both high virulence potential and antimicrobial resistance profiles. This systematic review presents the global distribution of clones of pneumococcal serotype 1, describing their circulating patterns in various regions in the world. A database search was conducted in Google Scholar, PubMed, Scopus, ScienceDirect, and Web of Science using keywords related to Streptococcus pneumoniae serotype 1. The inclusion criteria entailed peer-reviewed studies published in English describing the utilization of at least one molecular genotyping tool to identify S. pneumoniae serotype 1 clones based on their sequence types. Data extracted were managed and analyzed using Microsoft Excel 365 (Version 2108). Forty-three studies were finally included in the systematic review. A total of 103 MLST serotype 1 sequence types were identified in 48 countries. These clones were widely reported to be associated with invasive pneumococcal diseases. Globally, ST217 and ST306 clonal complexes (CC217 and CC306) were the predominant lineages of serotype 1 sequence types, exhibiting distinct continental distribution patterns. CC217, characterized by ST217, ST303, ST612, ST618, and ST3081, was predominant in Africa and Asia. ST306 clonal complex, which is grouped into ST306, ST304, and ST227 were mostly found in Europe, Oceania, North America, and some countries in South America. ST615 was predominant in Chile, Peru, and Argentina. The hypervirulence nature of serotype 1, coupled with its complex genetic diversity, poses a significant public health threat. Our findings emphasize the need for enhanced surveillance and targeted interventions to mitigate the spread of these hypervirulent clones, ultimately informing evidence-based strategies for disease prevention and control. Full article
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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<p>PRISMA flow diagram of the study selection process.</p>
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<p>Minimum spanning tree describing the relationships between the various clones identified in this review.</p>
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<p>Distribution of serotype 1 isolates identified in Africa.</p>
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<p>The global circulation of dominant clones of <span class="html-italic">S. pneumoniae</span> serotype 1.</p>
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<p>Distribution of serotype 1 isolates identified in Europe.</p>
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<p>Distribution of serotype 1 isolates identified in Asia.</p>
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<p>Distribution of serotype 1 isolates identified in the Americas.</p>
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<p>Distribution of serotype 1 isolates identified in Oceania.</p>
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16 pages, 4852 KiB  
Article
Vaal’s Microplastic Burden: Uncovering the Fate of Microplastics in Emfuleni Municipality’s Wastewater Treatment Systems, Gauteng, South Africa
by Tebogo Maleka, Richard Greenfield, Sudhakar Muniyasamy and Lee-Ann Modley
Sustainability 2025, 17(5), 2211; https://doi.org/10.3390/su17052211 - 4 Mar 2025
Viewed by 130
Abstract
Municipal wastewater treatment plants (WWTPs) in Gauteng, South Africa, are inadequately designed or optimized to effectively remove microplastics (MPs), resulting in approximately 80% of wastewater being discharged into aquatic ecosystems with insufficient treatment. This study evaluates the prevalence and abundance of MPs in [...] Read more.
Municipal wastewater treatment plants (WWTPs) in Gauteng, South Africa, are inadequately designed or optimized to effectively remove microplastics (MPs), resulting in approximately 80% of wastewater being discharged into aquatic ecosystems with insufficient treatment. This study evaluates the prevalence and abundance of MPs in municipal WWTPs and their subsequent introduction into receiving water bodies. Comprehensive sampling was conducted across three municipal WWTPs in the Emfuleni region of Gauteng province from October 2022 to July 2023. Initial MP identification and quantification were performed using light microscopy, while scanning electron microscope energy-dispersive X-ray spectroscopy (SEM/EDS) was employed to identify non-plastic particles and perform elemental analysis. The findings reveal significant seasonal variability in MP concentrations. The highest influent and effluent concentrations were recorded during October (spring), with influent values of 142 MPs/ℓ (WWTP 1), 124 MPs/ℓ (WWTP 2), and 132 MPs/ℓ (WWTP 3), and effluent concentrations of 120 MPs/ℓ (WWTP 1), 63 MPs/ℓ (WWTP 2), and 89 MPs/ℓ (WWTP 3). Conversely, the lowest MP concentrations were observed during April (autumn), with influent concentrations of 114 MPs/ℓ (WWTP 1), 141 MPs/ℓ (WWTP 2), and 78 MPs/ℓ (WWTP 3), and effluent concentrations of 99 MPs/ℓ (WWTP 1), 53 MPs/ℓ (WWTP 2), and 86 MPs/ℓ (WWTP 3). Fibers and filaments constituted the dominant MP morphology, primarily derived from polyester, nylon, and acrylic synthetic textiles. Dark-colored MPs, especially black, blue, and red particles, were predominant in the wastewater samples. This study underscores the critical role of WWTPs as conduits for MP contaminants into the environment and highlights the urgent need to develop and implement improved MP removal technologies in wastewater treatment systems. MP production is estimated to account for approximately 15–20% of total global plastic production, corresponding to an annual generation of approximately 52.5–80 million metric tons of MP. By addressing MP pollution, this research directly contributes to sustainability by promoting the protection of freshwater ecosystems, reducing anthropogenic pressures on aquatic biodiversity, and supporting the principles of sustainable development. The findings align with global and regional goals to enhance water quality management and promote sustainable urbanization practices in line with the United Nations Sustainable Development Goals (SDGs). Full article
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<p>Map of Gauteng, including the local municipalities (Source: <a href="http://www.municipality.gov.za" target="_blank">www.municipality.gov.za</a>. Accessed: 28 December 2024).</p>
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<p>Microplastic counts of influent and effluent wastewater sampled at Emfuleni Municipality in Gauteng South Africa. (<b>A</b>): Microplastic counts of influent and effluent wastewater sampled in October 2022 (Spring season), (<b>B</b>): Microplastic counts of influent and effluent wastewater sampled in January 2023 (Summer season), (<b>C</b>): Microplastic counts of influent and effluent wastewater sampled in April 2023 (Autum season) and (<b>D</b>): Microplastic counts of influent and effluent wastewater sampled in July 2023 (Winter season).</p>
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<p>Microplastic Particle Analysis of influent and effluent wastewater sampled at Emfuleni Municipality in Gauteng South Africa during the period October 2022 to July 2023.</p>
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<p>Colours of MPs observed in effluent wastewater samples collected at Emfuleni Municipality between October 2022 and July 2023.</p>
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<p>SEM image of an MP particle from a wastewater sample from WWTP1–3 at Emfuleni Municipality’s influent and effluent flow. The image shows the surface morphology of a filamentous MPs.</p>
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<p>SEM-EDS image of an MP particle from a wastewater sample from WWTP1 at Emfuleni Municipality’s influent flow.</p>
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<p>SEM-EDS image of an MP particle from a wastewater sample from WWTP2 (<b>A</b>) and WWTP3 (<b>B</b>) at Emfuleni Municipality, Gauteng, South Africa.</p>
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<p>FTIR analysis indication of the presence of PE in influent at WWTP 2 in Emfuleni Municipality, Gauteng, South Africa.</p>
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15 pages, 1751 KiB  
Review
Maximizing Photosynthesis and Plant Growth in African Legumes Through Rhizobial Partnerships: The Road Behind and Ahead
by Sanjay K. Jaiswal and Felix D. Dakora
Microorganisms 2025, 13(3), 581; https://doi.org/10.3390/microorganisms13030581 - 4 Mar 2025
Viewed by 121
Abstract
The interplay between soil rhizobial bacteria and leguminous plants, particularly in Africa, has a profound impact on photosynthetic efficiency and overall crop productivity. This review explores the critical role of rhizobia in enhancing photosynthesis through nitrogen fixation, a process crucial for sustainable agriculture. [...] Read more.
The interplay between soil rhizobial bacteria and leguminous plants, particularly in Africa, has a profound impact on photosynthetic efficiency and overall crop productivity. This review explores the critical role of rhizobia in enhancing photosynthesis through nitrogen fixation, a process crucial for sustainable agriculture. Rhizobial bacteria residing in root nodules provide legumes with symbiotic nitrogen that significantly boosts plant growth and photosynthetic capacity. Recent advances in molecular genomics have elucidated the genetic frameworks underlying this symbiosis, identifying key genes involved in root nodule formation and nitrogen fixation. Comparative genomics of Bradyrhizobium species have revealed seven distinct lineages, with diverse traits linked to nodulation, nitrogen fixation, and photosynthesis. Field studies across Africa demonstrate that rhizobial inoculation can markedly increase nodulation, nitrogen fixation, and grain yields, though outcomes vary depending on local soil conditions and legume species. Notable findings include enhanced nutrient uptake and photosynthetic rates in inoculated legumes compared with nitrate-fed plants. This review highlights the potential of utilizing indigenous rhizobia to improve photosynthesis and crop resilience. Future prospects involve leveraging genomic insights to optimize rhizobial inoculants and enhance legume productivity in water-limited environments. As climate change intensifies, integrating these advancements into agricultural practices could play a crucial role in improving food security and sustainable soil health in Africa. Full article
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<p>This diagram provides a schematic representation of how leguminous plants engage in a mutualistic relationship with <span class="html-italic">Rhizobium</span> to fix atmospheric nitrogen. The energy derived from photosynthesis drives the synthesis of photosynthates, which fuel root and nodule development and support the biological nitrogen fixation (BNF) process. Through this intricate biological system, the plant obtains essential nitrogen in a usable form ammonia, thereby enhancing its growth and productivity without reliance on external nitrogen fertilizers.</p>
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<p>This line graph presents a comparative analysis of physiological parameters across different crops and countries under two treatments: Inoculated and Uninoculated. The parameters considered in this analysis are Photosynthesis Rate (A) (μmol CO<sub>2</sub> m<sup>−2</sup> s<sup>−1</sup>), Stomatal Conductance (gs) (mol H<sub>2</sub>O m<sup>−2</sup> s<sup>−1</sup>), and Transpiration Rate (E) (mmol H<sub>2</sub>O m<sup>−2</sup> s<sup>−1</sup>). The trends in this graph shows that the inoculated treatment often shows higher values for the physiological parameters compared to the uninoculated treatment, indicating the possible benefits of inoculation on crop performance. Uninoculated Treatment shows lower values, suggesting a reduced physiological response in the absence of inoculation. Graph was constructed based on published data from Ibny et al. (2019) [<a href="#B13-microorganisms-13-00581" class="html-bibr">13</a>]; Gyogluu et al. (2018) [<a href="#B46-microorganisms-13-00581" class="html-bibr">46</a>]; Dlamini et al. (2021) [<a href="#B47-microorganisms-13-00581" class="html-bibr">47</a>]; Mbah et al. (2022) [<a href="#B5-microorganisms-13-00581" class="html-bibr">5</a>]; Simbine et al. (2021) [<a href="#B48-microorganisms-13-00581" class="html-bibr">48</a>]; Ngwenya et al. (2022) [<a href="#B8-microorganisms-13-00581" class="html-bibr">8</a>]; Gunununu et al. (2023) [<a href="#B49-microorganisms-13-00581" class="html-bibr">49</a>].</p>
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23 pages, 701 KiB  
Article
Does ICT Adoption Moderate the Impact of Entrepreneurship on Economic Growth in Africa?
by Afees Noah and Oladipo David
Adm. Sci. 2025, 15(3), 88; https://doi.org/10.3390/admsci15030088 - 4 Mar 2025
Viewed by 118
Abstract
Many African countries continue to face economic challenges even though the continent has much potential for economic progress. Entrepreneurship and ICT are one of the key forces behind economic change. However, little is known about how entrepreneurship, ICT, and economic growth affect economic [...] Read more.
Many African countries continue to face economic challenges even though the continent has much potential for economic progress. Entrepreneurship and ICT are one of the key forces behind economic change. However, little is known about how entrepreneurship, ICT, and economic growth affect economic resilience and development in Africa at the international level. Designing policies supporting a more resilient and inclusive African economy requires understanding this. This study, therefore, contributes to the existing literature by examining the direct and indirect impacts of entrepreneurship and ICT on economic growth in Africa. The study employs static and dynamic panel analyses to analyze panel data on 29 African countries from 2006 to 2020. The empirical results reveal that entrepreneurship positively influences economic growth in the long-run, but has a negative influence in the short-run, while ICT positively influences economic growth both in the short- and long-run. The moderating effects of ICT on the relationship between entrepreneurship and economic growth reveals that ICT positively strengthens the positive impact of entrepreneurship in Africa both in the short- and long-run. Furthermore, the causality results show that there is bidirectional among economic growth, entrepreneurship, and ICT adoption. Consistent with these findings, policy ramifications are highlighted. Full article
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<p>Trend in NBD and GDP. Source: authors’ processing.</p>
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<p>Causal relation flow.</p>
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17 pages, 1485 KiB  
Article
Co-Designing Tiyanjane, a Participatory Intervention to Promote Parental Involvement in the Education of Children with Disabilities in Malawi
by David John Musendo, Blessings Chirwa, Chisomo Kamata, Daksha Patel, Tracey Smythe and Sarah Polack
Disabilities 2025, 5(1), 26; https://doi.org/10.3390/disabilities5010026 - 3 Mar 2025
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Abstract
This paper describes the co-design of a participatory group intervention developed to promote and enhance parental involvement in supporting the education of children with disabilities in Malawi. The intervention was developed through participatory co-design workshops and consensus meetings involving 23 stakeholders, including parents, [...] Read more.
This paper describes the co-design of a participatory group intervention developed to promote and enhance parental involvement in supporting the education of children with disabilities in Malawi. The intervention was developed through participatory co-design workshops and consensus meetings involving 23 stakeholders, including parents, teachers, and community leaders. The Behaviour Change Wheel framework and the Delphi technique guided the intervention development process, ensuring theoretical robustness and contextual relevance. The proposed intervention, Tiyanjane (‘Let Us Unite’), includes facilitator and participant training and practical face-to-face sessions over 12 weeks. The intervention targets four key areas: developing family action plans, holding regular meetings, providing ongoing support at home and school, and facilitating training and information exchange. This participatory approach, involving a wide range of local stakeholders, offers valuable insights into the process and outcomes of co-developing culturally relevant and theoretically grounded interventions to address the needs of families with children with disabilities in low-resource settings. Future research should include an evaluation of the feasibility and acceptability of the intervention and examine its applicability in diverse sociocultural settings within LMICs (low- and middle-income countries). Full article
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<p>Behaviour Change Wheel. Adapted from Michie, et al. 2011 [<a href="#B44-disabilities-05-00026" class="html-bibr">44</a>], under the terms of the Creative Commons Attribution 2.0 License (CC BY 2.0).</p>
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<p>Iterative Design Phases for the Intervention Development Process.</p>
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<p>Stages in the BCW Intervention Development Process. Adapted from Michie, et al. 2011 [<a href="#B44-disabilities-05-00026" class="html-bibr">44</a>], under the terms of the Creative Commons Attribution 2.0 License (CC BY 2.0).</p>
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<p>Key Intervention Functions for Tiyanjane.</p>
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28 pages, 979 KiB  
Review
Deep Convolutional Neural Networks in Medical Image Analysis: A Review
by Ibomoiye Domor Mienye, Theo G. Swart, George Obaido, Matt Jordan and Philip Ilono
Information 2025, 16(3), 195; https://doi.org/10.3390/info16030195 - 3 Mar 2025
Viewed by 181
Abstract
Deep convolutional neural networks (CNNs) have revolutionized medical image analysis by enabling the automated learning of hierarchical features from complex medical imaging datasets. This review provides a focused analysis of CNN evolution and architectures as applied to medical image analysis, highlighting their application [...] Read more.
Deep convolutional neural networks (CNNs) have revolutionized medical image analysis by enabling the automated learning of hierarchical features from complex medical imaging datasets. This review provides a focused analysis of CNN evolution and architectures as applied to medical image analysis, highlighting their application and performance in different medical fields, including oncology, neurology, cardiology, pulmonology, ophthalmology, dermatology, and orthopedics. The paper also explores challenges specific to medical imaging and outlines trends and future research directions. This review aims to serve as a valuable resource for researchers and practitioners in healthcare and artificial intelligence. Full article
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<p>General architecture of a CNN, showing the progression from raw image input to feature extraction and final output through convolutional, pooling, and fully connected layers [<a href="#B31-information-16-00195" class="html-bibr">31</a>]. This structure forms the foundation for many DL models, including those used in medical image analysis.</p>
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<p>AlexNet architecture.</p>
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<p>VGGNet architecture.</p>
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<p>ResNet Architecture.</p>
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<p>DenseNet architecture [<a href="#B47-information-16-00195" class="html-bibr">47</a>].</p>
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9 pages, 211 KiB  
Study Protocol
Towards the Developing and Designing of an Intervention to Promote Prenatal Physical Activity Using MomConnect (mHealth): A Formative Protocol
by Uchenna Benedine Okafor, Daniel Ter Goon and Rudolph Leon van Niekerk
Methods Protoc. 2025, 8(2), 26; https://doi.org/10.3390/mps8020026 - 3 Mar 2025
Viewed by 146
Abstract
Background and aim: The use of mHealth, especially short-message text (SMS), has proven to be an effective intervention in promoting behavioral health outcomes in populations across different contexts and settings. While MomConnect, an mHealth technological device designed to enhance maternal and child health [...] Read more.
Background and aim: The use of mHealth, especially short-message text (SMS), has proven to be an effective intervention in promoting behavioral health outcomes in populations across different contexts and settings. While MomConnect, an mHealth technological device designed to enhance maternal and child health services in South Africa, offers various health-related contents aimed at improving maternal outcomes for pregnant and postpartum women, it currently lacks information on prenatal physical activity. However, physical activity and exercise during pregnancy is safe and beneficial for both the mother and the baby. This article outlines the protocol for designing and developing a prenatal physical activity and exercise text messaging content for the MomConnect device. To achieve this, the protocol aims to elucidate the preferences of prenatal physical activity and exercise text messages and ascertain the preferred amount of SMS messaging to inform the design of an intervention for the incorporation of prenatal physical activity and exercise text messages into the MomConnect device in South Africa. Methods: We will apply a user-centred design approach conducted in three phases. First, a scoping literature review and interviews with pregnant women will be conducted to inform the formative stage for developing a desirable prototype SMS. Secondly, healthcare providers and pregnant women will be interviewed to collate data on the preferred SMS. Lastly, a cross-sectional survey will be conducted to determine the preferred quantity of SMS messaging to be incorporated in the MomConnect device. Expected outcomes: A preferred or desirable prenatal physical activity and exercise SMS text message will inform the design of SMS text messages to be incorporated into the content of the MomConnect device to promote prenatal physical activity and exercise participation among women in the Eastern Cape Province. This study will develop a tailored mHealth intervention to improve prenatal physical activity participation and health behaviors among pregnant women in South Africa. Full article
(This article belongs to the Section Public Health Research)
24 pages, 475 KiB  
Article
Price Gaps and Volatility: Do Weekend Gaps Tend to Close?
by Marnus Janse van Rensburg and Terence Van Zyl
J. Risk Financial Manag. 2025, 18(3), 132; https://doi.org/10.3390/jrfm18030132 - 3 Mar 2025
Viewed by 155
Abstract
This study investigates weekend price gaps in three major stock market indices—the Dow Jones Industrial Average (DJIA), NASDAQ, and Germany’s DAX—from 2013 to 2023, using high-frequency (5 min) data to explore whether gap movements arise from random volatility or reflect systematic market tendencies. [...] Read more.
This study investigates weekend price gaps in three major stock market indices—the Dow Jones Industrial Average (DJIA), NASDAQ, and Germany’s DAX—from 2013 to 2023, using high-frequency (5 min) data to explore whether gap movements arise from random volatility or reflect systematic market tendencies. We examine 205 weekend gaps in the DJIA, 270 in NASDAQ, and 406 in the DAX. Two principal hypotheses guide our inquiry as follows: (i) whether price movements into the gap are primarily driven by increased volatility and (ii) whether larger gaps are associated with heightened volatility. Employing Chi-square tests for the independence and linear regression analyses, our results show no strong, universal bias towards closing gaps at shorter distances across all three indices. However, at medium-to-large distances, significant directional patterns emerge, particularly in the DAX. This outcome challenges the assumption that weekend gaps necessarily “fill” soon after they open. Moreover, larger gap sizes correlate with elevated volatility in both the DJIA and NASDAQ, underscoring that gaps can serve as leading indicators of near-term price fluctuations. These findings suggest that gap-based anomalies vary by market structure and geography, raising critical questions about the universality of efficient market principles and offering practical insights for risk management and gap-oriented trading strategies. Full article
(This article belongs to the Special Issue Machine Learning Based Risk Management in Finance and Insurance)
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<p>Hit rate comparison up to 990 points for DJIA (US30) showing flattening trends.</p>
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<p>Hit rate comparison up to 990 points for NASDAQ (US100) showing flattening trends.</p>
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<p>Hit rate comparison up to 990 points for Dax showing flattening trends.</p>
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<p>Focused view hit rate comparison for DJIA (US30).</p>
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<p>Focused view hit rate comparison for Dax.</p>
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<p>Focused view hit rate comparison for NASDAQ (US100).</p>
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27 pages, 2822 KiB  
Article
Biocontrol of Phytopathogens Using Plant Growth Promoting Rhizobacteria: Bibliometric Analysis and Systematic Review
by Bernardo Espinosa-Palomeque, Omar Jiménez-Pérez, Ricardo I. Ramírez-Gottfried, Pablo Preciado-Rangel, Alain Buendía-García, Gerardo Zapata Sifuentes, Maria A. Sariñana-Navarrete and Tomás Rivas-García
Horticulturae 2025, 11(3), 271; https://doi.org/10.3390/horticulturae11030271 - 3 Mar 2025
Viewed by 179
Abstract
Biocontrol has emerged as an effective strategy for managing plant pathogens and pests. The use of plant growth-promoting rhizobacteria (PGPR) as biocontrol agents offers a sustainable alternative, enhancing plant morphology, biochemistry, physiology, and secondary metabolism. This study conducts a bibliometric analysis and systematic [...] Read more.
Biocontrol has emerged as an effective strategy for managing plant pathogens and pests. The use of plant growth-promoting rhizobacteria (PGPR) as biocontrol agents offers a sustainable alternative, enhancing plant morphology, biochemistry, physiology, and secondary metabolism. This study conducts a bibliometric analysis and systematic review of PGPR-based biocontrol research from 2019 to 2023, using the Web of Science (WoS) database. A total of 2823 publications were identified, with a significant increase in scientific output since 2019. Original research articles dominated the field, with India, China, the USA, and Pakistan leading in publication volume. Key contributors included Babalola (North-West University, South Africa), Kloepper (Auburn University, USA), and Shen (Nanjing Agricultural University, China), each with at least 25 publications. Co-authorship analysis revealed four major research networks centered in India, China, Brazil, and Canada. Bacillus and Pseudomonas were the most studied PGPR genera, recognized for their roles as bioinoculants, bioremediators, and biostimulants, mitigating the negative impacts of synthetic fertilizers and pesticides. This analysis underscores the growing global focus on PGPR-based biocontrol and its potential for sustainable agriculture. Strengthening international collaboration and accelerating applied research on PGPR formulations will be critical for optimizing their efficacy and scalability in real-world agricultural systems. Full article
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<p>Direct and indirect mechanisms of PGPR, made in BioRender.</p>
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<p>Evolutionary trend of the number of publications (n = 2823) and citations (83,507) on PGPR as biocontrol agents. ■ = publications, ○ = citations.</p>
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<p>The annual growth rate of PGPR as biocontrol agents.</p>
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<p>(<b>a</b>) Relative growth rate and (<b>b</b>) doubling time of publications on PGPR as biocontrol agents. ● = doubling time, ○ = relative growth rate.</p>
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<p>Pattern of authorships and citations received on PGPR as biocontrol agents. ■ = publications, ○ = citations.</p>
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<p>Co-authorship network of the most cited authors with at least 10 publications on PGPR as biocontrol agents, made in VOSviewer. The size of the circles indicates the relevance of each author, while the colors represent research groups with high interaction. The lines reflect co-authorship, where their thickness indicates the frequency of collaboration and their length indicates the closeness in the scientific network.</p>
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<p>(<b>a</b>) Cloud of the 20 keywords with the highest co-occurrences, and (<b>b</b>) network of keywords per author with at least 10 co-occurrences on PGPR as biocontrol agents, made in VOSviewer.</p>
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<p>Co-authorship network across countries with at least 5 publications on PGPR as biocontrol agents, made in VOSviewer.</p>
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