WO2024119462A1 - Manifold assembly, fluid system and biochemical analysis and detection platform - Google Patents

Abstract

A manifold assembly, a fluid system and a biochemical analysis and detection platform. The manifold assembly comprises: a manifold body (101), which has one or more first external flow channel interfaces (102-109), one or more first internal flow channel interfaces (127-134), one or more second internal flow channel interfaces (120, 123, 125, 126, 135-138), one or more second external flow channel interfaces (118) and a plurality of internal flow channels (162-179), wherein the plurality of internal flow channels comprise one or more first internal flow channels (162-169) and one or more second internal flow channels (170-179), each first external flow channel interface (102-109) is in communication with at least one of the first internal flow channel interfaces (127-134) by means of the corresponding first internal flow channel (162-179), and each second external flow channel interface (118) is in communication with at least one of the second internal flow channel interfaces (120, 123, 125, 126, 135-138) by means of the corresponding second internal flow channel (170-179); and one or more internal flow channel control valves (110-117), wherein each internal flow channel control valve (110-117) is connected between at least one of the first internal flow channel interfaces (127-134) and at least one of the second internal flow channel interfaces (120, 123, 125, 126, 135-138), and is configured to control connection and disconnection between the first internal flow channel interfaces (127-134) and the second internal flow channel interfaces (120, 123, 125, 126, 135-138) connected thereto.

Description

Manifold assemblies, fluid systems and biochemical analysis detection platforms Technical Field

The present disclosure relates to the technical field of fluid systems, and in particular to a manifold assembly, a fluid system and a biochemical analysis and detection platform.

Background technique

In the related art, when performing biochemical analysis and testing, such as gene sequencing, it involves the switching of multiple reagents (or samples). To achieve the switching of different reagents (or samples), a reversing device (or reagent selection device), such as a rotary valve, a slider valve, etc., is mostly used between the reagent box (or sample box) and the slide. Multiple pipes are arranged between the reversing device and the slide, which results in a large amount of reagents and a high detection cost.

In the related art, in order to solve the problems of large reagent consumption and high detection cost, a carrier with integrated flow channel is proposed. The carrier is a consumable and needs to be replaced frequently. The carrier with integrated flow channel relies on external active valve control devices. When replacing the carrier, the carrier with integrated flow channel has a large number of interfaces that need to be connected immediately, and the sealing difficulty is relatively high. In order to ensure that multiple interfaces are well sealed, the manufacturing accuracy of the carrier with integrated flow channel needs to be improved. Therefore, the manufacturing cost of the carrier with integrated flow channel is high, the difficulty is great, and the reliability is poor.

Summary of the invention

The object of the present disclosure is to provide a manifold assembly, a fluid system and a biochemical analysis detection platform.

A first aspect of the present disclosure provides a manifold assembly, comprising:

a manifold body, comprising one or more first external flow channel interfaces, one or more first internal flow channel interfaces, one or more second internal flow channel interfaces, one or more second external flow channel interfaces, and a plurality of internal flow channels, wherein the plurality of internal flow channels include one or more first internal flow channels and one or more second internal flow channels, each of the first external flow channel interfaces is communicated with at least one of the first internal flow channel interfaces through the corresponding first internal flow channel, and each of the second external flow channel interfaces is communicated with at least one of the second internal flow channel interfaces through the corresponding second internal flow channel; and

One or more internal flow channel control valves, each of which is connected between at least one of the first internal flow channel interfaces and at least one of the second internal flow channel interfaces, and is configured to control the on/off of the first internal flow channel interfaces and the second internal flow channel interfaces connected thereto.

In some embodiments of the manifold assembly,

At least two of the first internal flow channels are connected to the corresponding first external flow channel interface through another first internal flow channel; and/or

At least two of the second internal flow channels are communicated with the corresponding second external flow channel interface through another second internal flow channel.

In some embodiments of the manifold assembly,

The internal flow channel control valve is a solenoid valve; and/or

The internal flow channel control valve is a reversing valve.

In some embodiments of the manifold assembly,

At least one of the internal flow channel control valves is disposed correspondingly to one of the first internal flow channel interfaces and one of the second internal flow channel interfaces; and/or

At least one of the internal flow channel control valves is disposed corresponding to one of the first internal flow channel interfaces and more than two of the second internal flow channel interfaces; and/or

At least one of the internal flow channel control valves is disposed corresponding to at least two of the first internal flow channel interfaces and one of the second internal flow channel interfaces; and/or

At least one of the internal flow channel control valves is disposed corresponding to at least two of the first internal flow channel interfaces and at least two of the second internal interfaces.

In some embodiments of the manifold assembly,

The one or more internal flow channels include a third internal flow channel connected to at least one of the second internal flow channels;

The manifold body comprises a third external flow channel interface, and the third external flow channel interface is connected to the third internal flow channel;

The manifold assembly includes a bypass control valve configured to control the on/off of the third internal flow passage connected thereto and the at least one second internal flow passage connected thereto.

In the manifold assembly of some embodiments, the bypass control valve includes three ports, two of the three ports are respectively connected to the two second internal flow passages, and the other is connected to the third internal flow passage, and the bypass control valve is configured to connect the two second internal flow passages and disconnect the third internal flow passage or connect one of the two second internal flow passages with the third internal flow passage and disconnect the other.

In some embodiments of the manifold assembly, the manifold body is a multi-manifold plate, wherein:

A plurality of the first external flow channel interfaces are arranged side by side on the same side of the multi-manifold plate and/or;

A plurality of the second external flow channel interfaces are arranged side by side on the same side of the multi-manifold plate; and/or

The plurality of first internal flow channel interfaces and the plurality of external flow channel interfaces are arranged side by side on the same side of the multi-manifold plate; and/or

A plurality of the internal flow channel control valves are arranged side by side on the same side of the multi-manifold plate.

A second aspect of the present disclosure provides a fluid system, comprising:

The manifold assembly according to the first aspect of the present disclosure;

a fluid storage device, comprising one or more reagent storage areas for storing reagents, wherein the reagent storage areas are connected to corresponding first external flow channel interfaces;

a carrier comprising at least one flow cell, each of the flow cells being connected to a corresponding second external flow channel interface of the manifold assembly; and

One or more power sources, each of the power sources is configured to drive the reagent from the reagent storage area to flow to the corresponding flow cell.

In the fluid system of some embodiments, the fluid system also includes a reversing valve, which is arranged between the fluid storage device and the one or more first external flow channel interfaces of the manifold body, and is configured to selectively connect one of the reagent storage areas connected to it with one of the first external flow channel interfaces.

In the fluid system of some embodiments, the fluid system further includes a bypass line configured to be connected to at least one internal flow channel of the plurality of internal flow channels so that the reagent entering the manifold body flows into the bypass line.

In the fluid system of some embodiments, the carrier includes more than two flow cells, and the fluid system includes more than two power sources arranged corresponding to the more than two flow cells.

In the fluid system of some embodiments, the fluid system further includes a waste liquid storage module, wherein the waste liquid storage module is configured to receive waste liquid in the flow cell.

A third aspect of the present disclosure provides a biochemical analysis detection platform, which includes the manifold assembly described in the first aspect of the present disclosure or the fluid system described in the second aspect of the present disclosure.

In the biochemical analysis detection platform of some embodiments, the biochemical analysis detection platform includes a gene sequencer, and the gene sequencer includes the manifold assembly or the fluid system. Based on the manifold assembly of the present disclosure, the manifold body and multiple internal flow channel control valves form a manifold assembly. Compared with the related art of the slide with integrated flow channel described in the background technology, the flow channel for the slide is integrated into the manifold assembly, and the connection relationship between the multiple internal flow channels of the manifold body is controlled by the internal flow channel control valve, so that the number of interfaces of the slide can be limited to the necessary number of interfaces matching the number of flow cells, which is conducive to improving the sealing performance of the interface of the slide and the connected fluid element, and because the number of slide interfaces is small, there is no need to put forward higher processing requirements for the slide, which is conducive to controlling the slide manufacturing cost and reducing the manufacturing difficulty. The various flow channels and flow channel control components for supplying reagents to the slide are integrated in the manifold assembly that does not need to be frequently replaced, so that the amount of reagents in the flow channel can be reduced under the premise of providing a reagent supply flow channel that meets the detection requirements.

The fluid system and biochemical analysis detection platform of the present disclosure have the advantages of the manifold assembly of the present disclosure.

BRIEF DESCRIPTION OF THE DRAWINGS

The drawings described herein are used to provide a further understanding of the present disclosure and constitute a part of the present disclosure. The illustrative embodiments of the present disclosure and their descriptions are used to explain the present disclosure and do not constitute an improper limitation on the present disclosure. In the drawings:

FIG. 1 is a schematic structural diagram of a manifold assembly according to an embodiment of the present disclosure.

2A and 2B are schematic cross-sectional views of the manifold body structure of the manifold assembly shown in FIG. 1 .

FIG. 3 is a schematic diagram of the principle of a fluid system according to an embodiment of the present disclosure.

FIG. 4 is a schematic diagram of a structural transverse cross-section of a manifold body of a manifold assembly according to another embodiment of the present disclosure.

5A and 5B are schematic cross-sectional views of the structure of a manifold body of a manifold assembly according to another embodiment of the present disclosure.

FIG6 is a schematic diagram of a structural transverse cross-section of a manifold body of a manifold assembly according to another embodiment of the present disclosure.

7A and 7B are schematic cross-sectional views of the structure of a manifold body of a manifold assembly according to another embodiment of the present disclosure.

FIG. 8 is a schematic structural diagram of a fluid system according to another embodiment of the present disclosure.

Detailed ways

The technical solutions in the embodiments of the present disclosure will be clearly and completely described below in conjunction with the drawings in the embodiments of the present disclosure. Obviously, the described embodiments are only part of the embodiments of the present disclosure, rather than all of the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is by no means intended to limit the present disclosure and its application or use. Based on the embodiments in the present disclosure, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present disclosure.

Unless otherwise specifically stated, the relative arrangement, numerical expressions and numerical values of the parts and steps set forth in these embodiments do not limit the scope of the present disclosure. At the same time, it should be understood that, for ease of description, the sizes of the various parts shown in the accompanying drawings are not drawn according to the actual proportional relationship. The technology, methods and equipment known to those of ordinary skill in the relevant art may not be discussed in detail, but in appropriate cases, the technology, methods and equipment should be considered as a part of the specification. In all examples shown and discussed here, any specific value should be interpreted as being merely exemplary, rather than as a limitation. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters represent similar items in the following drawings, and therefore, once a certain item is defined in an accompanying drawing, it does not need to be further discussed in subsequent drawings.

As shown in FIG. 1 to FIG. 8 , an embodiment of the present disclosure provides a manifold assembly, including a manifold body and one or more internal flow channel control valves.

The manifold body includes one or more first external flow channel interfaces, one or more first internal flow channel interfaces, one or more second internal flow channel interfaces, one or more second external flow channel interfaces and multiple internal flow channels, the multiple internal flow channels include one or more first internal flow channels and one or more second internal flow channels, each first external flow channel interface is connected to at least one first internal flow channel interface through a corresponding first internal flow channel, and each second external flow channel interface is connected to at least one second internal flow channel interface through a corresponding second internal flow channel.

Each internal flow channel control valve is connected between at least one first internal flow channel interface and at least one second internal flow channel interface, and is configured to control the on-off of the first internal flow channel interface and the second internal flow channel interface connected thereto.

In the disclosed embodiment, the manifold body and the multiple internal flow channel control valves form a manifold assembly. Compared with the related art of the slide with integrated flow channels described in the background art, the flow channels for the slide are integrated into the manifold assembly, and the connection relationship between the multiple internal flow channels of the manifold body is controlled by the internal flow channel control valve, so that the number of interfaces of the slide can be limited to the necessary number of interfaces that match the number of flow cells, which is beneficial to improve the sealing performance of the interface of the slide 140 and the connected fluid element, and because the number of slide interfaces is small, there is no need to put forward higher processing requirements for the slide, which is beneficial to control the slide manufacturing cost and reduce the manufacturing difficulty. The various flow channels and flow channel control components for supplying reagents to the slide are integrated into the manifold assembly that does not need to be frequently replaced, so that the amount of reagent in the flow channel can be reduced under the premise of providing a reagent supply flow channel that meets the detection requirements.

In some embodiments of the manifold assembly, at least two first internal flow channels are connected to the corresponding first external flow channel interface through another first internal flow channel, as shown in Figures 1 to 6; and/or at least two second internal flow channels are connected to the corresponding second external flow channel interface through another second internal flow channel, as shown in Figures 7A, 7B and 8. For example: as shown in Figures 1 to 6, at least two first internal flow channels are connected to the corresponding first external flow channel interface through another first internal flow channel; as shown in Figures 7A, 7B and 8, at least two second internal flow channels are connected to the corresponding second external flow channel interface through another second internal flow channel.

The above-mentioned arrangement of the internal flow channel utilizes tree-like branches to design the internal flow channel, which is beneficial to reducing the number of sealing surfaces between the manifold body 101 and external fluid components, thereby facilitating improved reliability.

In the manifold assembly of some embodiments, the internal flow channel control valve is a solenoid valve; and/or the internal flow channel control valve is a reversing valve. For example, in the embodiments shown in Figures 1 to 4 and 6, the internal flow channel control valve is a two-position two-way solenoid valve; in the embodiments shown in Figures 5A, 5B, 7A, 7B and 8, the internal flow channel control valve is a two-position three-way solenoid valve.

The use of a reversing valve as the internal flow control valve is conducive to the accurate switching of the valve position of the internal flow control valve to achieve the on-off of the corresponding interface. The use of a solenoid valve as the internal flow control valve is conducive to the miniaturization of the internal flow control valve, the high degree of integration of the fluid control function, the strong scalability of the number of interfaces, and the reduction of the weight and height of the manifold assembly, and the reduction of the requirements for the carrying capacity and motion control accuracy of the carrier motion platform.

In the manifold assembly of some embodiments, at least one internal flow channel control valve is arranged corresponding to a first internal flow channel interface and a second internal flow channel interface; and/or at least one internal flow channel control valve is arranged corresponding to a first internal flow channel interface and two or more second internal flow channel interfaces; and/or at least one internal flow channel control valve is arranged corresponding to two or more first internal flow channel interfaces and a second internal flow channel interface; and/or at least one internal flow channel control valve is arranged corresponding to two or more first internal flow channel interfaces and two or more second internal interfaces. For example: as shown in Figures 1 to 4 and 6, each internal flow channel control valve is arranged corresponding to a first internal flow channel interface and a second internal flow channel interface; as shown in Figures 5A and 5B, each internal flow channel control valve is arranged corresponding to two first internal flow channel interfaces and one second internal flow channel interface; as shown in Figures 7A and 7B, each internal flow channel control valve is arranged corresponding to a first internal flow channel interface and two second internal flow channel interfaces. In an embodiment not shown in the figure, an internal flow channel control valve can be connected to more first internal flow channel interfaces and second internal flow channel interfaces.

As shown in Figure 6, in some embodiments of the manifold assembly, one or more internal flow channels also include a third internal flow channel, which is connected to at least one second internal flow channel; one or more internal flow channels also include a third external flow channel interface, which is connected to the third internal flow channel; the manifold assembly also includes a bypass control valve, which is configured to control the opening and closing of the third internal flow channel.

The arrangement of the third internal flow channel, the third external flow channel interface and the bypass control valve is conducive to forming a bypass flow path in the fluid system where the manifold assembly is located, thereby increasing the operational flexibility of the fluid system.

As shown in Fig. 6, in some embodiments of the manifold assembly, the bypass control valve includes three ports, two of which are respectively connected to the two second internal flow passages, and the other is connected to the third internal flow passage, and the bypass control valve is configured to connect the two second internal flow passages while disconnecting the third internal flow passage or connect one of the two second internal flow passages with the third internal flow passage while disconnecting the other. In the embodiment shown in Fig. 6, the bypass control valve is, for example, a two-position three-way solenoid valve.

As shown in Figures 1 to 8, in some embodiments of the manifold assembly, the manifold body is a multi-manifold plate, and a plurality of first external flow channel interfaces are arranged side by side on the same side of the multi-manifold plate and/or; a plurality of second external flow channel interfaces are arranged side by side on the same side of the multi-manifold plate; and/or a plurality of first internal flow channel interfaces and a plurality of external flow channel interfaces are arranged side by side on the same side of the multi-manifold plate; and/or a plurality of internal flow channel control valves are arranged side by side on the same side of the multi-manifold plate.

As shown in Figures 3 and 8, the present disclosure also provides a kind of fluid system. Fluid system comprises fluid storage device 153, the manifold assembly of the present disclosure embodiment and slide 140 and one or more power sources 143. Fluid storage device 153 comprises one or more reagent storage areas for storing reagent, and reagent storage area is connected with the corresponding first external flow channel interface. Slide 140 comprises at least one flow cell 140A, and each flow cell 140A is connected with the corresponding second external flow channel interface of manifold assembly. Each power source 143 is configured to drive the reagent flow of reagent storage area to corresponding flow cell 140A.

The fluid system of the disclosed embodiment has the same advantages as the manifold assembly of the disclosed embodiment.

As shown in Figures 3 and 8, in the fluid system of some embodiments, the fluid system also includes a reversing valve 151, which is arranged between the fluid storage device 153 and one or more first external flow channel interfaces of the manifold 101, and is configured to selectively connect a reagent storage area connected to it with a first external flow channel interface.

As shown in FIG. 3 and FIG. 8 , in the fluid system of some embodiments, the fluid system further includes a bypass line 146 , which is configured to be connected to at least one of the plurality of internal flow passages so that the reagent entering the manifold body 101 flows into the bypass line 146 .

As shown in FIG. 3 and FIG. 8 , in the fluid system of some embodiments, the carrier 140 includes two or more flow cells 140A, and the fluid system includes two or more power sources 143 arranged corresponding to the two or more flow cells 140A.

As shown in FIG. 3 and FIG. 8 , in the fluid system of some embodiments, the fluid system further includes a waste liquid storage module 145 , which is configured to receive waste liquid and bubbles in the flow cell 140A and the flow path.

The present disclosure provides a biochemical analysis detection platform, including the manifold assembly of the present disclosure or the fluid system of the present disclosure. The biochemical analysis detection platform includes, for example, a gene sequencer, which includes the manifold assembly of the present disclosure or the fluid system of the present disclosure.

The biochemical analysis detection platform of the embodiment of the present disclosure has the same advantages as the manifold assembly and fluid system of the embodiment of the present disclosure.

The manifold assembly and the fluid system of the embodiment of the present disclosure are described in more detail below with reference to FIGS. 1 to 8 .

As shown in FIGS. 1 to 3 , the manifold assembly 100 is an integrated solenoid valve manifold assembly, including a manifold body 101 and one or more internal flow channel control valves 110 to 117 .

The manifold body 101 is used to realize the integration of multiple internal flow channels, and if necessary, the required functional devices, such as sensors, etc., can also be integrated. The manifold body 101 has multiple internal flow channels and multiple interfaces. In this embodiment, the manifold body 101 is implemented in the form of a multi-manifold plate with multiple internal flow channels and multiple interfaces. The multiple interfaces include one or more first external flow channel interfaces, one or more first internal flow channel interfaces, one or more second internal flow channel interfaces, and one or more second external flow channel interfaces. The multiple internal flow channels include one or more first internal flow channels and one or more second internal flow channels. Each first external flow channel interface is connected to at least one first internal flow channel interface through the corresponding first internal flow channel, and each second external flow channel interface is connected to at least one second internal flow channel interface through the corresponding second internal flow channel.

The shapes of the internal flow channels of the manifold body 101 can be various. For example, the cross-section perpendicular to the fluid flow direction can be a circular cross-section, a D-shaped cross-section, a square cross-section, a trapezoidal cross-section, etc. Along the fluid flow direction, the internal flow channel can be a uniform cross-section flow channel or a variable cross-section flow channel. The variable cross-section flow channel can be, for example, a trumpet-shaped flow channel.

In the embodiments shown in FIGS. 1 to 3 , one or more first external flow channel interfaces include external flow channel interfaces 102 to 109; one or more first internal flow channel interfaces include internal flow channel interfaces 127 to 134; one or more second internal flow channel interfaces include internal flow channel interfaces 123, 125, 126 and 135 to 138; one or more second external flow channel interfaces include external flow channel interface 118; one or more first internal flow channels include internal flow channels 162 to 169; one or more second internal flow channels include internal flow channels 170 to 179; one or more internal flow channel control valves include internal flow channel control valves 110 to 117. Multiple internal flow channels can be arranged as one layer or multiple layers, that is, multiple flow channels can be on one plane, two planes or more planes. Corresponding to the arrangement of multiple internal flow channels, multiple interfaces can also be arranged as one layer or multiple layers.

Among the plurality of internal flow channels, the internal flow channel 179 is a common flow channel directly connected to the external flow channel interface 118. The internal flow channels 170 to 177 are all connected to the internal flow channel 179, and the internal flow channels 170 to 179 form a tree-like branch structure.

Each external flow channel interface 102-109 is used to realize the connection between the manifold body 101 and the fluid device outside the manifold assembly 100. For example, the external flow channel interface 102-109 can be connected to a reagent box, a sample box or other fluid devices, such as a rotary valve, a solenoid valve, etc. The external flow channel interface 102-109 can be used as both a fluid inlet and an outlet.

The external flow channel interface 118 is used to connect the internal flow channel 179 with the flow pool 140A of the carrier 140. The second external flow channel interface 118 can be one or more. The number of the second external flow channel interface 118 in Figures 1 to 3 is one.

Each of the internal flow channel interfaces 127 to 134 is used to be connected to a port of an internal flow channel control valve to achieve connection with the external flow channel of the manifold assembly 100; each of the internal flow channel interfaces 120, 123, 125, 126, 135 to 138 is used to be connected to another port of an internal flow channel control valve to achieve communication with the internal flow channel 179 of the manifold body 101 and the external flow channel interface 118, and then achieve connection with the flow pool 140A of the carrier 140.

The internal flow channel control valves 110 to 117 are used to realize the switching (or opening and closing) of the internal flow channels connected thereto. In this embodiment, the internal flow channel control valves 110 to 117 are two-position two-way electromagnetic switching valves.

As shown in Figures 1 to 3, multiple external flow channel interfaces 102 to 109 are respectively connected to multiple internal flow channel interfaces 134, 133, 132, 131, 130, 129, 128, and 127 through internal flow channels 162 to 169; one port of the internal flow channel control valve 110 to 117 is respectively connected to the internal flow channel interfaces 134, 133, 132, 131, 130, 129, 128, and 127; another port of the internal flow channel control valve 110 to 117 is respectively connected to the internal flow channel interfaces 135 to 138, 120, 123, 125, and 126; multiple internal flow channel interfaces 135 to 138, 120, 123, 125, and 126 are respectively connected to the internal flow channel 179 through the internal flow channels 170 to 177; the internal flow channel 179 is connected to the external flow channel interface 118.

The internal flow channel 179 is a common flow channel through which more than two reagents flow. In this embodiment, each internal flow channel 170 to 177 connected to the internal flow channel interface 120, 123, 125, 126, 135-138 can be directly merged or branched to the internal flow channel 179. For example, the pipelines connected to the internal flow channel interface 120, 123, 125, 126, 135-138 can be merged into one flow channel in pairs, and then merged into the internal flow channel 179. In this embodiment, the internal flow channel 175 connected to the internal flow channel interface 123 and the internal flow channel 176 connected to the internal flow channel interface 125 are first merged into the internal flow channel 178, and the internal flow channel 178 is then connected to the internal flow channel 179, and the other internal flow channels 170 to 174 and 177 are directly connected to the internal flow channel 179.

In an embodiment not shown, the internal flow channels connected to the internal flow channel interfaces 120 , 123 , 125 , 126 , and 135 - 138 may converge into one point in the internal flow channel 179 .

As shown in FIGS. 1 to 3 , the main working process of the fluid system having the aforementioned manifold assembly includes:

1. Process of reagent fluid flowing into the carrier 140

When the internal flow channel control valve 117 is in the first position and is in an open state, the internal flow channel interface 126 is connected to the internal flow channel interface 127, and the fluid (reagent/sample/gas) enters the manifold body 101 from the first external flow channel interface 109, flows through the internal flow channel 169 and the internal flow channel control valve 117, and then flows out from the external flow channel interface 118 through the internal flow channel interface 126, the internal flow channel 177 and the internal flow channel 179.

When the internal flow channel control valve 116 is in the first position and is in an open state, the internal flow channel interface 125 is connected to the internal flow channel interface 128, and the fluid (reagent/sample/gas) enters the manifold body 101 along the internal flow channel interface 108, flows through the internal flow channel 168 and the internal flow channel control valve 116, and then flows out from the external flow channel interface 118 through the internal flow channel interface 125 and the internal flow channels 176, 178 and 179.

2. Process of reagents flowing into the bypass

When the internal flow channel control valve 116 and the internal flow channel control valve 111 are in the first position and in an open state, the internal flow channel interface 125 is connected to the internal flow channel interface 128, and the internal flow channel interface 136 is connected to the internal flow channel interface 133. The fluid (reagent/sample/gas) enters the manifold body 101 along the first external flow channel interface 108, flows through the internal flow channel 168, the internal flow channel control valve 116, the internal flow channel 176, the internal flow channel 178, the internal flow channel 179, the internal flow channel 171, and the internal flow channel control valve 111 in sequence, and flows out of the manifold body 101 from the external flow channel interface 103.

The above only describes the working process of the embodiment of the present disclosure through the working process of some internal flow channels, some interfaces and some internal flow channel control valves. The working process of the remaining internal flow channels, interfaces and internal flow channel control valves not described can refer to the previous description.

In addition, the fluid may also flow in the reverse direction along the above path under the action of the driving source.

According to the manifold assembly 100 of the above embodiment, it can be known that the manifold assembly 100 of the embodiment of the present disclosure adopts an integrated flow channel, which can reduce the common volume, reduce the reagent usage, and reduce the detection (such as gene sequencing) cost; the number of interfaces of the carrier 140 of the fluid system where the manifold assembly 100 is located can be limited to the necessary number of interfaces that match the number of flow cells, which is beneficial to improve the sealing performance of the interface of the carrier 140 and the connected fluid elements, and because the number of carrier interfaces is small, there is no need to put forward higher processing requirements for the carrier, which is beneficial to control the carrier manufacturing cost and reduce the manufacturing difficulty; the internal flow channel control valve is adopted, which can reduce the weight and height of the carrier 140 motion platform, reduce the requirements for the carrier 140 load-bearing capacity and motion control accuracy, and achieve reliable sealed connection.

FIG3 is a fluid system having the manifold assembly 100 shown in FIG1 to FIG3. As shown in FIG3, the fluid system includes a power source 143, a waste liquid storage module 145, a fluid storage device 153, a pipette assembly, a fluid pipeline assembly, a manifold assembly, and a reversing valve 151. The fluid pipeline assembly includes a pipeline 141, a pipeline 144, and a pipeline 146. The pipette assembly includes a plurality of reagent pipelines, such as a reagent pipeline 152.

The manifold assembly includes a manifold body 101 and a plurality of internal flow channel control valves 110 to 117. The details thereof may refer to the above description of the manifold assembly 100.

In the embodiment shown in FIG. 3 , the slide 140 has a flow cell 140A.

The power source 143 is used to realize the movement of samples or reagents in the fluid system. The power source 143 can generate a certain form of pressure, including negative pressure or positive pressure, depending on the direction required for fluid transportation. The power source 143 has multiple fluid interfaces to realize the transportation of different fluids. For example, the power source 143 is connected to the pipeline 141 or the pipeline 146, and the transportation of samples, reagents or cleaning fluids can be realized. It is connected to the pipeline 144 to realize the discharge of waste liquid. The power source 143 can include a hydraulic unit in the form of a peristaltic pump, a plunger pump, a syringe pump, a gear pump, a diaphragm pump, etc., and can also include an air power unit in the form of a vacuum pump, a diaphragm pump, an air compressor, a gas tank with a certain positive or negative pressure, etc. The power source 143 can include a reversing valve 142, wherein the reversing valve 142 can be a reversing device such as a solenoid valve, a rotary valve, a pneumatic reversing valve, an electro-hydraulic reversing valve, a manual reversing valve, a piezoelectric valve, a pinch valve, a rotary valve, a rotary valve, a rotary valve, etc.

The waste liquid storage module 145 has one or more interfaces for storing or discharging all or part of the waste liquid of the fluid system. When the fluid system belongs to an instrument, such as a gene sequencer, the waste liquid storage module 145 can be arranged inside the instrument body or outside the instrument body, or directly discharged to the laboratory waste liquid treatment system using the interface of the waste liquid storage module 145. The waste liquid storage module 145 can be without a container or can include one or more containers. According to functional requirements, the waste liquid storage module 145 can also include a sensor for liquid detection, an air purification device or a secondary overflow prevention device, etc.

The fluid storage device 153 is used to store liquids such as reagents, samples, cleaning fluids, etc. required by the fluid system for biochemical detection (such as gene sequencing). Each liquid can correspond to a reagent storage area. In this embodiment, the fluid storage device 153 includes a plurality of reagent bottles, and the receiving portion of each reagent bottle constitutes a reagent storage area.

The pipette assembly is used to extract the liquid in the fluid storage device 153 and transport it outward. The pipette assembly can include a reagent tube, a reagent needle or a manifold. The pipette assembly can also include a filter, a bubble sensor, etc.

The fluid pipeline assembly is used to achieve the connection of fluid devices and the delivery of fluids. The fluid pipeline assembly may include detection devices such as pressure sensors, flow sensors, and bubble sensors arranged on the pipeline to detect the state of the fluid system.

The reversing valve 151 is used to switch the connection relationship between different pipelines of the pipette assembly and different first external flow channel interfaces of the manifold body 101, so that the sample or reagent can be transported according to the set path. For example, the internal flow channel control valve 117 has two ports, one of which can be connected to the slide 140, and the other port can be connected to the fluid storage device 153, or it can be connected to the fluid storage device 153 through other fluid devices, so that the reversing valve 151 can control whether the fluid storage device 153 is connected to the slide 140. The reversing valve 151 can be a reversing device such as a solenoid valve, a rotary valve, a pneumatic reversing valve, an electro-hydraulic reversing valve, a manual reversing valve, a piezoelectric valve, a pinch valve, a rotary valve, a rotary valve, a rotary cutting valve, etc. In this embodiment, the reversing valve 151 is a rotary valve. The rotary valve has 8 holes, and the 8 holes are respectively connected to a reagent bottle.

As shown in Figures 1 to 3, the main working process of the above fluid system includes:

1. Working process of reagent flowing into the slide 140

The reversing valve 151 is switched to the 8th hole position, the internal flow channel control valve 117 is in the first position, and the reversing valve 142 of the power source 143 is switched to communicate with the pipeline 141. The power source 143 generates negative pressure, and the reagent flows from the corresponding reagent bottle of the fluid storage device 153 along the reagent pipeline 152 of the pipette assembly, the reversing valve 151, the reagent pipeline 150, the internal flow channel 169, the internal flow channel control valve 117, the internal flow channel 177 and the internal flow channel 179 from the external flow channel interface 118 into the slide 140.

2. Working process of reagent flow into bypass

The reversing valve 151 is switched to the 8th hole position, the internal flow channel control valve 117 and the internal flow channel control valve 111 are in the first position, and the reversing valve 142 of the power source 143 is switched to communicate with the pipeline 146. The power source 143 generates negative pressure, and the reagent enters the pipeline 146 as a bypass from the corresponding reagent bottle of the fluid storage device 153 along the reagent pipeline 152, the reversing valve 151, the reagent pipeline 150, the internal flow channel 169, the internal flow channel control valve 117, the internal flow channel 177, the internal flow channel 179, the internal flow channel 171 and the internal flow channel control valve 111 of the pipette assembly from the external flow channel interface 103.

3. Working process of reagent discharge

The reversing valve 142 of the power source 143 is switched to communicate with the pipeline 144 , and the power source 143 generates positive pressure to discharge the reagent into the waste liquid storage module 145 .

The above only schematically describes the working process of the fluid system of the embodiment of the present disclosure through the working process of the fluid system corresponding to the working process of part of the internal flow channels, part of the interfaces and part of the internal flow channel control valves of the manifold assembly 100. The working process corresponding to the working process of the remaining internal flow channels, interfaces and internal flow channel control valves of the manifold assembly 100 of the fluid system not described can refer to the previous description. The fluid system of the embodiment of the present disclosure has the same advantages as the manifold assembly 100 of the embodiment of the present disclosure. Figure 4 is a structural schematic diagram of the manifold body of the manifold assembly of another embodiment of the present disclosure. The following only describes the differences between this embodiment and the manifold assembly 100 of the embodiment shown in Figures 1 to 3. Some structures in Figure 4 that are the same as those of the embodiment shown in Figures 1 to 3 are not marked with figure marks, and the figure marks of these structures can refer to the corresponding figure marks of the embodiment shown in Figures 1 to 3.

As shown in FIG4 , in this embodiment, the number of one or more second external flow channel interfaces is two, including an external flow channel interface 118 and an external flow channel interface 202. The external flow channel interface 118 and the external flow channel interface 202 are connected to the end of the internal flow channel 179 through the internal flow channel 201 and the internal flow channel 203, respectively. That is, the internal flow channel 179 as a common flow channel is forked to both sides to form two second internal flow channels, namely the internal flow channel 201 and the internal flow channel 203, and the ends of the internal flow channel 201 and the internal flow channel 203 away from the internal flow channel 179 are respectively connected to the two external flow channel interfaces 118 and the external flow channel interface 202 as the second external flow channel interfaces to adapt to the carriers 140 of different channels. The manifold assembly of the embodiment shown in FIG4 can adapt to the carrier 140 of two channels. The carrier 140 of two channels includes two flow pools 140A arranged side by side, and the two flow pools 140A respectively enter and exit fluids.

In addition, in the embodiment shown in FIG. 4 , the connection method of the internal flow channels 175 to 178 as the second internal flow channels is different from that of the internal flow channels 175 to 178 in the embodiment shown in FIGS. 1 to 3 . In the embodiment shown in FIG. 4 , the internal flow channels 175 to 177 are all connected to the internal flow channel 178, and the internal flow channels are connected to the internal flow channel 179. That is, three second internal flow channels (internal flow channels 175 to 177) first converge into another second internal flow channel (internal flow channel 178) and then converge into the second internal flow channel (internal flow channel 179) as the common flow channel. The remaining internal flow channels 170 to 174 as the second internal flow channels each converge into the internal flow channel 179 separately.

For the unexplained parts of the manifold assembly and fluid system of the embodiment corresponding to Figure 4, reference may be made to the relevant description of the preceding embodiments.

FIG5A and FIG5B are schematic diagrams of the structure of a manifold body of a manifold assembly of another embodiment of the present disclosure. The following only describes the differences between this embodiment and the manifold assembly 100 of the embodiment shown in FIG1 to FIG3. Some structures in FIG5A and FIG5B that are the same as those in the embodiment shown in FIG1 to FIG3 are not marked with reference numerals, and the reference numerals of these structures can refer to the corresponding reference numerals of the embodiment shown in FIG1 to FIG3.

As shown in Figures 5A and 5B, in the embodiment of the present disclosure, compared to the embodiments shown in Figures 1 to 3, in the manifold assembly, one or more first external flow channel interfaces also include external flow channel interfaces 212 to 219, one or more first internal flow channel interfaces also include internal flow channel interfaces 222 to 229, and one or more first internal flow channels also include internal flow channels 232 to 239.

In the embodiment of the present disclosure, the internal flow passage control valves 110 to 117 with two ports in the embodiment shown in Figures 1 to 3 are replaced by internal flow passage control valves with three ports. The internal flow passage control valves in this embodiment are, for example, two-position three-way solenoid valves. The first port of the three ports of each internal flow passage control valve is connected to a second internal flow passage port, and the other two ports are each connected to a first internal flow passage port. Each internal flow passage control valve is configured so that the second internal flow passage port connected to it can be selectively connected to one of the two first internal flow passage ports connected to it, and disconnected from the other. In this embodiment, one port of each of the eight internal flow passage control valves is connected to the internal flow passage ports 135 to 138, 120, 123, 125 and 126 in sequence; one of the other two ports of the eight internal flow passage control valves is connected to the internal flow passage interfaces 222 to 229 in sequence, and the other of the other two ports of the eight internal flow passage control valves is connected to the internal flow passage interfaces 134, 133, 132, 131, 130, 129, 128 and 127 in sequence.

In this embodiment, when the internal flow channel control valve connecting the internal flow channel interfaces 228, 128 and 125 and the internal flow channel control valve connecting the internal flow channel interfaces 229, 127 and 126 are both in the first position, the internal flow channel interface 128 is connected to 125 and the internal flow channel interface 228 is disconnected, and the internal flow channel interface 127 is connected to 126 and the internal flow channel interface 229 is disconnected; when the internal flow channel control valve connecting the internal flow channel interfaces 228, 128 and 125 and the internal flow channel control valve connecting the internal flow channel interfaces 229, 127 and 126 are both in the second position, the internal flow channel interface 228 is connected to 125 and the internal flow channel interface 128 is disconnected, and the internal flow channel interface 229 is connected to 126 and the internal flow channel interface 127 is disconnected.

The control relationships of other internal flow channel control valves can all refer to the internal flow channel control valves connected to the internal flow channel interfaces 228 , 128 and 125 and the internal flow channel control valves connected to the internal flow channel interfaces 229 , 127 and 126 .

For the unexplained parts of the manifold assembly and the fluid system of the embodiments corresponding to FIG. 5A and FIG. 5B , reference may be made to the relevant description of the aforementioned embodiments.

FIG6 is a schematic diagram of the structure of a manifold body of a manifold assembly according to another embodiment of the present disclosure. The following only describes the differences between this embodiment and the manifold assembly 100 of the embodiment shown in FIGS. 1 to 3. Some structures in FIG6 that are the same as those in the embodiment shown in FIGS. 1 to 3 are not marked with reference numerals, and the reference numerals of these structures can refer to the corresponding reference numerals of the embodiment shown in FIGS. 1 to 3.

As shown in FIG6, compared with the manifold assembly of the embodiment shown in FIGS. 1 to 3, in the manifold assembly of the embodiment of the present disclosure, one or more internal flow channels also include a third internal flow channel. In this embodiment, the third internal flow channel is the internal flow channel 244. The internal flow channel 244 is connected to at least one second internal flow channel; the one or more internal flow channels also include a third external flow channel interface. The third external flow channel interface is the external flow channel interface 245. The external flow channel interface 245 is connected to the internal flow channel 244. The manifold assembly also includes a bypass control valve (not shown), which is configured to control the opening and closing of the internal flow channel 244 connected thereto and at least one second internal flow channel connected thereto.

The bypass control valve includes three ports. Two of the three ports of the bypass control valve are respectively connected to the two internal flow passages 179 and the internal flow passage 241 as the second internal flow passage, and the other is connected to the internal flow passage 244 as the third internal flow passage. The bypass control valve is configured to make the internal flow passage 179 and the internal flow passage 241 communicate with each other while the internal flow passage 244 is disconnected, or to make one of the internal flow passage 179 and the internal flow passage 241 communicate with the internal flow passage 244 while the other is disconnected. In the embodiment shown in FIG. 6 , the bypass control valve is, for example, a two-position three-way solenoid valve.

As shown in Fig. 6, the one or more interfaces further include internal flow channel interfaces 240, 242 and 243. The one or more second internal flow channels further include internal flow channel 241, internal flow channel 244 is connected to internal flow channel interface 240, both ends of internal flow channel 241 are respectively connected to internal flow channel interface 242 and external flow channel interface 118, internal flow channel 179 is connected to internal flow channel interface 243, and the three ports of the bypass control valve are respectively connected to internal flow channel interfaces 240, 241 and 243, thereby respectively connecting to internal flow channel 244, internal flow channel 179 and internal flow channel 241.

The manifold assembly of the embodiment shown in Fig. 6 can realize the reagent bypass function. Of course, the sample or reagent can also be loaded from the third external flow channel interface through the third external flow channel interface, the third internal flow channel and the bypass control valve.

For the unexplained parts of the manifold assembly and the fluid system of the embodiment corresponding to FIG6 , reference may be made to the relevant description of the aforementioned embodiments.

Fig. 7A and Fig. 7B are schematic diagrams of the structure of a manifold body of a manifold assembly of another embodiment of the present disclosure. Fig. 8 is a schematic diagram of the structure of a fluid system of another embodiment of the present disclosure. The fluid system shown in Fig. 8 adopts the manifold assembly of the embodiment shown in Fig. 7A and Fig. 7B. The manifold body of the manifold assembly of the embodiment shown in Figs. 7A to 8 is different from the manifold body of the embodiment shown in Figs. 1 to 3 in that the manifold body of the embodiment shown in Figs. 1 to 3 includes a plurality of first external flow channel inlets and a second external flow channel interface, while the manifold body of the embodiment shown in Figs. 7A to 8 includes a first external flow channel inlet and a plurality of second external flow channel interfaces.

As shown in FIG. 7A , FIG. 7B and FIG. 8 , the manifold assembly is an integrated solenoid valve manifold assembly, including a manifold body and a plurality of internal flow channel control valves.

The manifold body has multiple internal flow channels and multiple interfaces. In this embodiment, the manifold body is implemented in the form of a multi-manifold plate with multiple internal flow channels and multiple interfaces. The multiple interfaces include one or more first external flow channel interfaces, one or more first internal flow channel interfaces, one or more second internal flow channel interfaces, and one or more second external flow channel interfaces. The multiple internal flow channels include one or more first internal flow channels and one or more second internal flow channels. Each first external flow channel interface is connected to at least one first internal flow channel interface through a corresponding first internal flow channel, and each second external flow channel interface is connected to at least one second internal flow channel interface through a corresponding second internal flow channel.

In the embodiment shown in FIG. 7A , FIG. 7B and FIG. 8 , one or more first external flow channel interfaces include external flow channel interfaces 301 and 341 to 348; one or more first internal flow channel interfaces include internal flow channel interfaces 311 to 318 and 321 to 328; one or more second internal flow channel interfaces include internal flow channel interfaces 331 to 338; one or more second external flow channel interfaces include external flow channel interfaces 351 to 358; one or more first internal flow channels include internal flow channels 361 to 367, 371 to 378 and 381 to 388; one or more second internal flow channels include internal flow channels 391 to 398; one or more internal flow channel control valves include eight internal flow channel control valves 302 to 309. Multiple internal flow channels can be arranged as one layer or multiple layers, that is, multiple flow channels can be on one plane, two planes or more planes. Corresponding to the arrangement of multiple internal flow channels, multiple interfaces can also be arranged as one layer or multiple layers.

In the embodiment shown in Fig. 7A, Fig. 7B and Fig. 8, among the plurality of internal flow channels, the internal flow channels 361 to 367 and 371 to 378 as the first internal flow channels form a tree-like branch structure. As shown in Fig. 7A, Fig. 7B and Fig. 8, the internal flow channel 361 is connected to the external flow channel interface 301. The end of the internal flow channel 361 away from the external flow channel interface 301 branches into the internal flow channel 362 and the internal flow channel 363. The internal flow channel 362 branches into the internal flow channels 364 and 365. The internal flow channel 363 branches into the internal flow channels 366 and 367. The internal flow channel 364 branches into the internal flow channels 371 and 372. The internal flow channel 365 branches into the internal flow channels 373 and 374. The internal flow channel 366 branches into 375 and 376. The internal flow channel 367 branches into the internal flow channels 377 and 378. The internal flow passages 371 to 378 are sequentially connected to the internal flow passage interfaces 311 to 318 serving as first internal flow passage interfaces.

In the embodiment shown in Fig. 7A, Fig. 7B and Fig. 8, the external flow channel interface 301 is used to realize the connection between the manifold body and the fluid device outside the manifold assembly. For example, the external flow channel interface 301 can be connected to a reagent box, a sample box or other fluid devices, such as a rotary valve, a solenoid valve, etc. The external flow channel interface 301 can be used as both a fluid inlet and an outlet. The external flow channel interfaces 341 to 348 can be connected to an external reagent or a sample box. The external flow channel interfaces 351 to 358 are used to connect to the flow cell 140A of the slide 140.

As shown in Figures 7A, 7B and 8, multiple external flow channel interfaces 341 to 348 are connected to multiple internal flow channel interfaces 331 to 338 through internal flow channels 381 to 388 respectively; multiple external flow channel interfaces 351 to 358 are connected to multiple internal flow channel interfaces 321 to 328 through internal flow channels 391 to 398 respectively.

The internal flow channel control valve is used to achieve the switching (or opening and closing) of the internal flow channel connected to it.

In the embodiment of the present disclosure, each of the internal flow channel control valves 302 to 309 has three ports. The internal flow channel control valves 302 to 309 of the present embodiment are, for example, two-position three-way solenoid valves. One of the three ports of each internal flow channel control valve 302 to 309 is connected to a second internal flow channel port, and the other two ports are each connected to a first internal flow channel port. Each internal flow channel control valve 302 to 309 is configured so that a second internal flow channel port connected thereto can be selectively connected to one of the two first internal flow channel ports connected thereto, and the other is disconnected. In the present embodiment, one port of the eight internal flow channel control valves 302 to 309 is sequentially connected to the internal flow channel ports 321 to 328; one of the other two ports of the eight internal flow channel control valves 302 to 309 is sequentially connected to the internal flow channel interfaces 311 to 318, and the other of the other two ports of the eight internal flow channel control valves is sequentially connected to the internal flow channel interfaces 331 to 338.

As shown in Fig. 8, the fluid system includes a plurality of power sources 143, a waste liquid storage module 145, a fluid storage device 153, a pipette assembly, a fluid pipeline assembly, a manifold assembly and a reversing valve 151. The fluid pipeline assembly includes pipeline 141 and pipeline 144. The pipette assembly includes a plurality of reagent pipelines, such as reagent pipeline 152.

The manifold assembly includes a manifold body and a plurality of internal flow channel control valves 302 to 309. The details thereof can be found in the above description of the manifold assembly shown in FIG. 7A to FIG. 8.

In the fluid system of the embodiment shown in FIG8 , the carrier 140 has a plurality of flow cells 140A. The flow cell 140A is a place where sequencing is performed for biochemical reactions, and the size of each flow cell 140A can be exactly the same or different in size. All the flow cells 140A in the plurality of flow cells 140A can be gathered into one inlet or one outlet, or some of the flow cells 140A can be gathered into one inlet or one outlet, while the other part of the flow cells 140A have independent outlets. In this embodiment, the plurality of flow cells 140A are independent of each other. The plurality of flow cells 140A are respectively connected to the external flow channel interfaces 351 to 358 of the manifold body.

The power source 143 is used to realize the movement of the sample or reagent in the fluid system. The power source 143 can generate a certain form of pressure, including negative pressure or positive pressure, depending on the direction required for fluid transportation. The power source 143 has multiple fluid interfaces to realize the transportation of different fluids. For example, the power source 143 is connected with the pipeline 141, and the transportation of the sample, reagent or cleaning solution can be realized, and it is connected with the pipeline 144 to realize the discharge of waste liquid. The power source 143 can include a hydraulic unit in the form of a peristaltic pump, a plunger pump, a syringe pump, a gear pump, a diaphragm pump, etc., and can also include an air power unit in the form of a vacuum pump, a diaphragm pump, an air compressor, a gas tank with a certain positive pressure or negative pressure, etc. The power source 143 can include a reversing valve 142, wherein the reversing valve 142 can be a reversing device such as a solenoid valve, a rotary valve, a pneumatic reversing valve, an electro-hydraulic reversing valve, a manual reversing valve, a piezoelectric valve, a pinch valve, a rotary valve, a rotary valve, a rotary valve, a rotary cutting valve. In the present embodiment, multiple power sources 143 are arranged one by one with multiple flow cells 140A of the slide 140. Multiple power sources 143 are connected to the flow pool 140A of the corresponding carrier 140 through their reversing valves 142 and multiple pipes 141.

The waste liquid storage module 145 has multiple interfaces for storing or discharging all or part of the waste liquid of the fluid system. According to functional requirements, the waste liquid storage module 145 may also include a sensor for liquid detection, an air purification device or a secondary overflow prevention device. In this embodiment, the waste liquid storage module 145 includes multiple interfaces, and multiple power sources 143 are connected to the corresponding interfaces of the waste liquid storage module 145 through their reversing valves 142 and multiple pipelines 144.

Fluid storage device 153 is used to store liquids such as reagents, samples, cleaning solutions required by fluid system during biochemical detection (such as gene sequencing). Each liquid can correspond to a reagent storage area. In the present embodiment, fluid storage device 153 includes multiple reagent bottles, and the accommodating portion of each reagent bottle constitutes a reagent storage area. 16 reagent bottles of fluid storage device 153 are shown in Figure 8, wherein 8 reagent bottles on the right side are connected to the external flow channel interface 301 of manifold body through 8 reagent pipelines and reversing valve 151, and 8 reagent bottles on the left side are respectively connected to the external flow channel interface 341 to 348 of manifold body through other 8 reagent pipelines.

The pipette assembly is used to extract the liquid in the fluid storage device 153 and transport it outward. The pipette assembly can include a reagent tube, a reagent needle or a manifold. The pipette assembly can also include a filter, a bubble sensor, etc.

The fluid pipeline assembly is used to achieve the connection of fluid devices and the delivery of fluids. The fluid pipeline assembly may include detection devices such as pressure sensors, flow sensors, and bubble sensors arranged on the pipeline to detect the state of the fluid system.

The reversing valve 151 is used to realize the switching of the connection relationship between the different pipelines of the pipette assembly and the different first external flow channel interfaces of the manifold body, so that the sample or reagent can be transported according to the set path. For example, the internal flow channel control valve 309 has three ports, one of which can be connected to the slide 140, another port can be connected to the fluid storage device 153, and the third port can be connected to the reagent bottle. Thus, the reversing valve 151 can be used to control whether the fluid storage device 153 is connected to the slide 140. The reversing valve 151 can be a reversing device such as a solenoid valve, a rotary valve, a pneumatic reversing valve, an electro-hydraulic reversing valve, a manual reversing valve, a piezoelectric valve, a pinch valve, a rotary valve, a rotary valve, and a rotary cutting valve. In the present embodiment, the reversing valve 151 is a rotary valve. The rotary valve has 8 holes, and the 8 holes are respectively connected to the 8 reagent bottles on the right side of the storage device 153.

The following only describes the working process of the embodiment of the present disclosure based on the working process of the flow channel related to the internal flow channel control valve 309 . The working process of the flow channels related to other internal flow channel control valves can all refer to the working process of the flow channels related to the internal flow channel control valve 309 .

When the reversing valve 151 rotates to the 8th hole position and the internal flow channel control valve 309 is in the first position (lower position in Figure 8), the internal flow channel interfaces 318 and 328 are connected, and the internal flow channel interface 338 is disconnected, so that the reagent from the reagent bottle connected to the reagent pipeline 152 passes through the reversing valve 151, the reagent pipeline 150, the external flow channel interface 301, the internal flow channel 361, the internal flow channel 363, the internal flow channel 367, the internal flow channel 378, the internal flow channel interface 318, the internal flow channel control valve 309, the internal flow channel interface 328, the internal flow channel 398 and the external flow channel interface 358 into the flow pool 140A of the carrier 140 connected to the external flow channel interface 358. When the internal flow channel control valve 309 is in the second position (upper position in Figure 8), the internal flow channel interfaces 338 and 328 are connected, the internal flow channel interface 318 is disconnected, and the reagent in the reagent bottle connected to the external flow channel interface 348 of the manifold body enters the flow pool 140A of the carrier 140 connected to the external flow channel interface 358 through the external flow channel interface 348, the internal flow channel 388, the internal flow channel interface 338, the internal flow channel control valve 309, the internal flow channel interface 328, the internal flow channel 398 and the external flow channel interface 358.

In this embodiment, the reagent bottles connected to the external flow channel interfaces 341 to 348 of the manifold body can be used independently, and the calling time can be shortened. In addition, the loss of the reversing valve is reduced.

The “reagents” mentioned in all the above embodiments should be understood as all fluids including pure water and gas.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present disclosure rather than to limit it. Although the present disclosure has been described in detail with reference to the preferred embodiments, ordinary technicians in the relevant field should understand that the specific implementation methods of the present disclosure can still be modified or some technical features can be replaced by equivalents without departing from the spirit of the technical solution of the present disclosure, which should be included in the scope of the technical solution for protection of the present disclosure.

Claims (14)

1. A manifold assembly comprising:

   a manifold body, comprising one or more first external flow channel interfaces, one or more first internal flow channel interfaces, one or more second internal flow channel interfaces, one or more second external flow channel interfaces, and a plurality of internal flow channels, wherein the plurality of internal flow channels include one or more first internal flow channels and one or more second internal flow channels, each of the first external flow channel interfaces is communicated with at least one of the first internal flow channel interfaces through the corresponding first internal flow channel, and each of the second external flow channel interfaces is communicated with at least one of the second internal flow channel interfaces through the corresponding second internal flow channel; and

   One or more internal flow channel control valves, each of which is connected between at least one of the first internal flow channel interfaces and at least one of the second internal flow channel interfaces, and is configured to control the on/off of the first internal flow channel interfaces and the second internal flow channel interfaces connected thereto.
2. The manifold assembly of claim 1, wherein:

   At least two of the first internal flow channels are connected to the corresponding first external flow channel interface through another first internal flow channel; and/or

   At least two of the second internal flow channels are communicated with the corresponding second external flow channel interface through another second internal flow channel.
3. The manifold assembly of claim 1, wherein:

   The internal flow channel control valve is a solenoid valve; and/or

   The internal flow channel control valve is a reversing valve.
4. The manifold assembly of claim 1, wherein:

   At least one of the internal flow channel control valves is disposed correspondingly to one of the first internal flow channel interfaces and one of the second internal flow channel interfaces; and/or

   At least one of the internal flow channel control valves is disposed corresponding to one of the first internal flow channel interfaces and more than two of the second internal flow channel interfaces; and/or

   At least one of the internal flow channel control valves is disposed corresponding to at least two of the first internal flow channel interfaces and one of the second internal flow channel interfaces; and/or

   At least one of the internal flow channel control valves is disposed corresponding to at least two of the first internal flow channel interfaces and at least two of the second internal interfaces.
5. The manifold assembly of claim 1, wherein:

   The one or more internal flow channels include a third internal flow channel connected to at least one of the second internal flow channels;

   The manifold body comprises a third external flow channel interface, and the third external flow channel interface is connected to the third internal flow channel;

   The manifold assembly includes a bypass control valve configured to control the on/off of the third internal flow passage connected thereto and the at least one second internal flow passage connected thereto.
6. The manifold assembly according to claim 5, wherein the bypass control valve comprises three ports, two of the three ports are respectively connected to the two second internal flow passages, and the other is connected to the third internal flow passage, and the bypass control valve is configured to connect the two second internal flow passages while disconnecting the third internal flow passage or connect one of the two second internal flow passages with the third internal flow passage while disconnecting the other.
7. The manifold assembly according to any one of claims 1 to 6, wherein the manifold body is a multi-manifold plate, wherein:

   A plurality of the first external flow channel interfaces are arranged side by side on the same side of the multi-manifold plate and/or;

   A plurality of the second external flow channel interfaces are arranged side by side on the same side of the multi-manifold plate; and/or

   The plurality of first internal flow channel interfaces and the plurality of external flow channel interfaces are arranged side by side on the same side of the multi-manifold plate; and/or

   A plurality of the internal flow channel control valves are arranged side by side on the same side of the multi-manifold plate.
8. A fluid system, comprising:

   The manifold assembly according to any one of claims 1 to 7;

   a fluid storage device (153), comprising one or more reagent storage areas for storing reagents, wherein the reagent storage areas are connected to corresponding first external flow channel interfaces;

   A carrier (140) comprising at least one flow cell (140A), each of the flow cells (140A) being connected to a corresponding second external flow channel interface of the manifold assembly; and

   One or more power sources (143), each of the power sources (143) is configured to drive the reagent flow from the reagent storage area to the corresponding flow cell (140A).
9. The fluid system according to claim 8 further includes a reversing valve (151), which is arranged between the fluid storage device (153) and the one or more first external flow channel interfaces of the manifold body, and is configured to selectively connect one of the reagent storage areas connected to it with one of the first external flow channel interfaces.
10. The fluid system according to claim 8 or 9, further comprising a bypass line (146), wherein the bypass line (146) is configured to be connected to at least one of the plurality of internal flow passages so that the reagent entering the manifold body (101) flows into the bypass line (146).
11. A fluid system according to claim 8 or 9, wherein the carrier (140) includes more than two flow pools (140A), and the fluid system includes more than two power sources (143) arranged corresponding to the more than two flow pools (140A).
12. The fluid system according to claim 8 or 9, further comprising a waste liquid storage module (145), wherein the waste liquid storage module (145) is configured to receive waste liquid in the flow cell (140A).
13. A biochemical analysis detection platform, comprising a manifold assembly according to any one of claims 1 to 7 or a fluid system according to any one of claims 8 to 12.
14. The biochemical analysis and detection platform according to claim 13, wherein it includes a gene sequencer, and the gene sequencer includes the manifold assembly or the fluid system.

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