WO2018032889A1 - Insecticidal composition having synergistic effect - Google Patents

Abstract

Provided is an insecticidal composition having a synergistic effect. The composition comprises two active components A and B. The active component A is niclosamide or a salt thereof. The active component B is selected from abamectin. The weight ratio of the component A to the component B is 1:40-40:1. Also provided is a preparation method for the composition and an application thereof. The test results show that the insecticidal composition has a remarkable synergistic effect. Moreover, the dosage can be reduced, and prevention costs can be lowered. The insecticidal composition can effectively control Pomacea canaliculata, snails, and Oncomelania snails. Co-formulating insecticides with different action mechanisms and action approaches can effectively reduce the dosage of each single insecticide and has good effects in broadening the insecticidal spectrum, reducing insects' resistance to insecticides, improving the control effect, and other aspects.

Description

Synergistic insecticide composition Technical field

The present invention relates to an insecticidal and acaricidal composition having improved properties, and more particularly to an insecticidal and acaricidal composition comprising a spirulina or a salt thereof and avermectin, and belongs to the field of pesticide preparations.

Background technique

Niclosamide is a salicylamide derivative. Its anti-insect mechanism is to inhibit the mitochondrial oxidative phosphorylation process in the cells, and the production of ATP is reduced, which causes the aphid's head section and adjacent segments to deteriorate. The intestinal wall is exfoliated with the feces. Ineffective for eggs. Death tablets are easily digested by proteases in the intestine, releasing eggs and posing a risk of cysticercosis. In agriculture, it is mainly used to kill snails, snails, beef mites, snails, pork mites, fish mites, mites, mites, mites.

Abamectin is a class of 16-membered macrolide compounds with insecticidal, acaricidal and nematicidal activity produced by fermentation of Streptomyces avermitilis in Streptomyces. Abamectin has stomach poisoning and contact killing on mites and insects and cannot kill eggs. The mechanism of action differs from that of general insecticides in that it interferes with neurophysiological activities and stimulates the release of gamma-aminobutyric acid, which inhibits the nerve conduction of arthropods. Adult mites, nymphs and insect larvae are paralyzed after contact with avermectin, inactive, not feeding, and die after 2 to 4 days. The avermectin lethal effect is slower because it does not cause rapid dehydration of insects.

Studies have shown that long-term repeated and specific application of an active compound to control pests will in many cases lead to increased resistance of pests. To reduce pest resistance, mixtures of different active compounds are currently commonly used to control pests. By combining active compounds having different action mechanisms, resistance can be delayed, application amount can be reduced, control effect can be improved, and control cost can be reduced.

Summary of the invention

The object of the present invention is to screen the insecticides of two different action principles for the resistance problem of the insecticide in practical application, so as to improve the effect of prevention and treatment, reduce the application amount and expand the scope of use.

To achieve the above object, the present invention provides a group of insecticidal and acaricidal compositions comprising two active ingredients A and B, active ingredient A is snail or a salt thereof, and active ingredient B is avermectin Prime. The snail amine salt compound is preferably a snail amine ethanolamine salt.

The inventors found through experiments that the above-mentioned insecticidal composition has obvious synergistic effect, and the application amount is reduced, which can reduce the use cost. The compounds containing component A and component B have different structural types and different mechanisms of action. The combination of the two can broaden the spectrum of action and can delay the generation of insect resistance to a certain extent, and component A and component B There is no cross-resistance.

The present invention provides a preparation method comprising the two active ingredients A and B compositions and an application for controlling crop pests in the agricultural field.

It is characterized in that the composition comprises two active components A and B, and the weight ratio between the two components is 1:40 to 40:1, preferably the weight ratio is 1:30 to 30:1, more preferably the weight. The ratio is 1:20 to 20:1. In some embodiments, the two active components A and B can be selected from 40:1, 39:1, 38:1, 37:1, 36:1, 35:1, 34:1, 33:1, 32 : 1, 31: 1, 30: 1, 29: 1, 28: 1, 27: 1, 26: 1, 25: 1, 24: 1, 23: 1, 22: 1, 21: 1, 20: 1 , 19:1, 18:1, 17:1, 16:1, 15:1, 14:1, 13:1, 12:1, 11:1, 10:1, 9:1, 8:1, 7 : 1, 6: 1, 5: 1, 4: 1, 3: 1, 2: 1, 1:1, 1: 2, 1:3, 1:4, 1:5, 1:6, 1:7 , 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1 :20, 1:21, 1:22, 1:23, 1:24, 1:25, 1:26, 1:27, 1:28, 1:29, 1:30, 1:31, 1:32 More specific ratios such as 1:33, 1:34, 1:35, 1:36, 1:37, 1:38, 1:39, 1:40, etc., can also be composed of any two ratios above. The selection is made within the range, which can be understood as a weight ratio, and may also include a molar ratio.

The active ingredient snailamine salt compound in the present invention is preferably a snailamine ethanolamine salt.

The composition further contains an auxiliary agent, a solvent, and a carrier auxiliary component which are usually used in a pesticide preparation to prepare various dosage forms suitable for use in agriculture.

The compositions of the present invention may be formulated with the active ingredients together with adjuvants or carrier adjunct ingredients in any of the dosage forms permitted in agriculture.

The mass of the active ingredient in the composition of the invention may range from 2 to 95%, preferably from 5 to 80%.

The composition of the present invention can be prepared into various dosage forms by a known method, and the active ingredient and the auxiliary agent, such as a solvent, a solid carrier, and, if necessary, can be uniformly mixed and ground together with the surfactant to prepare a desired preparation. Dosage form.

Suitable auxiliaries may be solid or liquid, they are usually materials commonly used in the processing of dosage forms, such as natural or regenerated minerals, solvents, dispersants, wetting agents, adhesives, thickeners, binders or fertilizers. .

The above solvent may be selected from aromatic hydrocarbons, preferably containing from 8 to 12 carbon atoms, such as a mixture of xylenes or substituted benzenes, phthalates such as dibutyl phthalate or dicaprylic acid, aliphatic hydrocarbons such as rings. Alkenes or paraffins, alcohols and glycols and their ethers and esters, such as ethanol, ethylene glycol, ethylene glycol monomethyl; ketones, such as cyclohexanone, highly polar solvents such as N-methyl-2 Pyrrolidone, dimethyl sulfoxide or dimethylformamide, and vegetable or vegetable oils such as soybean oil.

The above solid carriers, such as those used in powders and dispersibles, are typically natural mineral fillers such as talc, kaolin, montmorillonite or activated clay. In order to manage the physical properties of the composition, it is also possible to add a highly dispersible silicic acid or a highly dispersible adsorbent polymer carrier, such as a particulate adsorbent carrier or a non-adsorbing carrier, and a suitable particulate adsorbent carrier is porous, such as pumice, bentonite or Bentonite; a suitable non-adsorbing carrier such as calcite or sand. In addition, a large amount of pre-granulated materials of inorganic or organic nature can be used as a carrier, in particular dolomite.

Suitable surfactants according to the chemical nature of the active ingredient in the composition of the present invention are lignosulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, alkaline earth metal or amine salts, alkylarylsulfonates, alkyl groups Sulfates, alkyl sulfonates, fatty alcohol sulphates, fatty acids and sulfated fatty alcohol glycol ethers, as well as condensates of sulfonated naphthalene and naphthalene derivatives with formaldehyde, naphthalene or naphthalene sulfonic acid with phenol and formaldehyde Condensate, polyoxyethylene octyl phenyl ether, ethoxylated isooctyl phenol, octyl phenol, nonyl phenol, alkyl aryl polyglycol ether, tributyl benzene polyglycol ether, three Stearyl phenyl polyglycol ether, alkyl aryl polyether alcohol, ethoxylated castor oil, polyoxyethylene alkyl ether, ethylene oxide condensate, ethoxylated polyoxypropylene, lauric acid poly Glycol ether acetal, sorbitol ester, lignin sulfite waste liquor and methyl cellulose.

The method of application of the compositions of the present invention comprises the use of the compositions of the present invention in aerial parts of plants, including leaves or foliage. The plant's growth site, such as wheat, may be wetted with a liquid formulation of the composition, or the composition may be applied to the soil in solid form, such as in the form of granules (soil application), the composition may be passed from the soil to the plant through the roots of the plant. In vivo (systemic action).

These compositions may be applied only by the active ingredient or may be used in combination with the additives, so that the composition of the present invention can be prepared into various dosage forms such as wettable powders, suspending agents, water-dispersible granules, aqueous emulsions, microemulsions. . Depending on the nature of the compositions and the intended purpose and environmental conditions to which the compositions are to be applied, the compositions may be applied by spraying, misting, dusting, spreading or pouring, and the like.

The two active ingredients in the compositions of the present invention exhibit synergistic effects and are more pronounced than the individual activities of the individual compounds. The synergistic effect is manifested by allowing for a reduced application rate, a broader insecticidal spectrum, a quicker effect, a more durable control effect, better control of the pest by only one or a few applications, and a widening of the possible application intervals. These characteristics are very important to solve the ubiquitous resistance problem of a single pesticide.

The composition of the present invention can be applied to the control of crop pests in the agricultural field, especially in the control of snails, snails or snails. On the other hand, the insecticidal composition of the present invention is applied to various crops such as wheat, cotton, corn, soybean, vegetable, fruit tree and the like, and has good activity, and pests and scales of the sucker. Hymenoptera, such as hibiscus, leaf miner, thrips, aphids, etc., also have good activity.

The other characteristics of this product are mainly as follows: 1. It can be applied to various possible application methods such as spraying and mixing; 2. The chemical composition of the two single agents of the composition is very different, and the mechanism of action is completely different. , there is no cross-resistance, The problem of resistance caused by the two single agents being used alone can be delayed; 3. The compounding of the composition of the invention has obvious synergistic effect, and is safe and effective against crops. It has been proved by experiments that the composition of the invention has stable chemical properties, remarkable synergistic effect, and exhibits obvious synergistic effect and complementary effect on the control object.

detailed description

In order to make the objects, technical solutions and advantages of the present invention more comprehensible, the present invention will be further described in detail below with reference to the embodiments. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the scope of the invention.

The percentages in all formulations in the following examples are percentages by weight. The processing techniques of the various formulations of the compositions of the present invention are all prior art and may vary depending on the circumstances.

First, the dosage form preparation example

(1) Processing and examples of suspending agents

The active ingredients of spirulina and avermectin are mixed with the dispersing agent, stabilizer, antifoaming agent and solvent in the proportion of the formula, ground in a sanding kettle, and then sent to a homomixer for mixing. Get the finished product evenly.

Example 1: 31% snail-killing avermectin suspension

30% of snails, 1% of avermectin, 8% of sodium lignosulfonate, 4% of bentonite, 1% of xanthan gum, 2% of silicone defoamer, and 100% of soybean oil.

Example 2: 31% streptozotomin and avermectin suspension

1% of chlorpyrifos, 30% of avermectin, 7% of naphthalenesulfonic acid formaldehyde condensate, 5% of diatomaceous earth, 2% of magnesium silicate, and 100% of dichloroethane.

(2) Processing and examples of microemulsions

After the active ingredient snail and avermectin are completely dissolved in the solvent, stirring is carried out, and an emulsifier, a synergist and an antifreezing agent are added at the same time. After the above system is mixed, the water is slowly added to the above mixture. In the liquid, after fully stirring, different amounts of microemulsion can be formulated.

Example 3: 11% snail-killing avermectin microemulsion

10% of snailamine, 1% of avermectin, 23% of acetone, 5% of alkyl ethoxylate and nonylphenol ethoxylate, 2% of sodium nitrophenolate, 1% of propylene glycol, 2% of urea, water Make up to 100%.

Example 4: 11% snail-killing avermectin microemulsion

1% of snailamine, 10% of avermectin, 22% of azone, 3% of fatty acid polyoxyethylene ester and phenethyl phenol ethoxylate, glycerol 4%, methanol 13%, water up to 100% .

Example 5: 10% snailamine·avermectin microemulsion

Destranamine 5%, avermectin 5%, alkylbenzene sulfonate and alkylnaphthalene sulfonate 15%, ketone 1%, propylene glycol 1%, ethyl acetate 11%, water to 100%.

(3) Processing and examples of wettable powders

The active ingredients of spirulina and avermectin are uniformly mixed with a solvent and an auxiliary agent under mechanical stirring, and pulverized by an ultrafine pulverizer to obtain a wettable powder.

Example 6: 21% snailamine ethanolamine salt avermectin wettable powder

20% of snailamine ethanolamine salt, 1% of avermectin, 4% of pull-open powder, 5% of sodium dodecylbenzenesulfonate, 6% of diatom, and 100% of attapulgite.

Example 7: 21% snail-killing avermectin wettable powder

1% of spirulina, 20% of avermectin, 4% of butyl hydroxyanisole, 5% of calcium lignosulfonate, 3% of alkyl polyoxyethyl ether sulfonate, 2% of bentonite, white carbon black 4 %, diatomaceous earth is made up to 100%.

Example 8 41% of snailamine ethanolamine salt · avermectin wettable powder

40% of snailamine ethanolamine salt, 1% of avermectin, 8% of sodium lignosulfonate, 3% of polyoxyethylene octylphenyl ether, 7% of kaolin, and 100% of diatomaceous earth.

Example 9 41% snailamine ethanolamine salt · avermectin wettable powder

1% of snailamine ethanolamine salt, 40% of avermectin, 6% of sodium lignosulfonate, 2% of silica, 5% of sodium dodecylbenzenesulfonate, and 100% of attapulgite.

Second, the efficacy test

(1) Determination of indoor virulence activity

Example 1 Determination of the toxicity test of spirulina and avermectin

1.1 test drug

Sebacmin, avermectin

1.2 Test insect source

Citrus red spider

1.3 Determination method

Mortality was calculated and corrected for control mortality to determine the lethal concentration.

1.4 Data Analysis

Separin according to the percentage of active ingredient: avermectin ratio: 40:1, 30:1, 20:1, 10:1, 1:1, 1:10, 1:20, 1:30, 1: 40 Experiments were carried out to calculate the virulence index-level co-toxicity coefficient (CTC) of the drug according to Sun Yunpei method.

Measured virulence index (ATI) = (standard drug LC50 / test drug LC50) * 100

Theoretical virulence index (TTI) = A virulence index * Percentage of A in the mixture + B virulence index * Percentage of B in the mixture

Co-toxicity coefficient (CTC) = [mixture measured virulence index (ATI) / mixed theory virulence index (TTI)] * 100

CTC ≤ 80, the composition showed antagonism, 80 < CTC < 120, the composition showed an additive effect, CTC ≥ 120, and the composition showed synergistic effect.

Table 1 Analysis of the results of determination of virulence of snails, avermectin and its different ratios

The results (Table 1) showed that the control effect of the combination of snail and avermectin on the snail was significantly improved, indicating that the combination of the two had a significant preventive effect. It has been found that when the combination of snail and avermectin is not within the scope of the requirements of the present invention, it is difficult for the two to have a synergistic effect on the snail.

Example 2 Determination of venom toxicity test by combination of snailamine ethanolamine salt and avermectin

Table 2 Determination of the toxicity of spirulina ethanolamine salt and avermectin

The results (Table 2) showed that the control effect of the combination of the snail and the avermectin on the snail was significantly improved, indicating that the two snails have a significant preventive effect. It has been found that when the compounding of the snail amine ethanolamine salt and avermectin is not within the scope of the requirements of the present invention, it is difficult for the snail to have a synergistic effect on the snail.

(2) Field efficacy test

The field efficacy test was repeated 4 times for each treatment, and the whole plant was evenly sprayed at the peak of the pest occurrence. Each treatment plot immediately took 5 points, and 10 plants were calibrated at each point. The number of insect populations was investigated before application, and the number of live insects was recorded 5 days after application, and the rate of reduction of insects was calculated, that is, the control effect. At the same time, the test crops were investigated for signs of phytotoxicity. Determine the safety of the agent.

Expected control effect (%) = X + Y-XY / 100 (where X, Y is a single dose control)

Table 3. Field control trial results of the combination of snail and avermectin on winter melon snails

The results of the determination (Table 3) showed that the synergistic effect of the combination of snail and avermectin on the prevention and treatment of snails was significantly improved, indicating that the two pairs of snails had significant synergistic effects. Throughout the test, no phytotoxicity to winter melon growth was observed in the range of the amount of the composition of the present invention, indicating that the composition of the present invention is safe for melon growth.

Claims (10)

1. A synergistic insecticidal composition, characterized in that the composition comprises two active components A and B, wherein active component A is snail or its salt, and active component B is A The avermectin has a weight ratio between the two components of 1:40 to 40:1.
2. The composition according to claim 1, characterized in that the weight ratio of the active ingredient, spiroxamine or a salt thereof, to the active ingredient avermectin is from 1:30 to 30:1.
3. The composition according to claim 2, characterized in that the weight ratio of the active ingredient, lysine or a salt thereof, to the active ingredient avermectin is from 1:20 to 20:1.
4. A composition according to Claim 1, 2 or 3, characterized in that the active ingredient snailamine salt compound is a snailamine ethanolamine salt.
5. The composition according to claim 1, characterized in that the composition further contains an auxiliary agent, a solvent, and a carrier auxiliary component which are usually used in a pesticide preparation to prepare various dosage forms suitable for agricultural use.
6. A composition according to claim 5 wherein the composition is in a dosage form of any of the agriculturally acceptable dosage forms.
7. The composition according to claim 5, characterized in that the active ingredient in the composition has a mass content of from 2 to 95%.
8. The composition according to claim 5, characterized in that the composition is in the form of a wettable powder, a suspension or an aqueous emulsion.
9. Use of the composition of claim 1, 2 or 3 for controlling crop pests in the agricultural sector.
10. Use of the composition of claim 1, 2 or 3 for controlling snails, snails or snails.