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WO2012057567A3 - Klotho 유전자를 포함하는 피부 활성물질 탐색용 키트 및 이를 이용하여 피부 활성물질을 탐색하는 방법 - Google Patents

Klotho 유전자를 포함하는 피부 활성물질 탐색용 키트 및 이를 이용하여 피부 활성물질을 탐색하는 방법 Download PDF

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Publication number
WO2012057567A3
WO2012057567A3 PCT/KR2011/008132 KR2011008132W WO2012057567A3 WO 2012057567 A3 WO2012057567 A3 WO 2012057567A3 KR 2011008132 W KR2011008132 W KR 2011008132W WO 2012057567 A3 WO2012057567 A3 WO 2012057567A3
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WO
WIPO (PCT)
Prior art keywords
screening
skin
activating substances
kit
same
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Application number
PCT/KR2011/008132
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English (en)
French (fr)
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WO2012057567A2 (ko
Inventor
최향태
손의동
이진영
나용주
배지현
Original Assignee
주식회사 아모레퍼시픽
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 주식회사 아모레퍼시픽 filed Critical 주식회사 아모레퍼시픽
Priority to US13/881,312 priority Critical patent/US20130252247A1/en
Priority to JP2013536527A priority patent/JP6186279B2/ja
Priority to EP11836659.0A priority patent/EP2634262B1/en
Publication of WO2012057567A2 publication Critical patent/WO2012057567A2/ko
Publication of WO2012057567A3 publication Critical patent/WO2012057567A3/ko

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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6881—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids from skin
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/148—Screening for cosmetic compounds
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Cosmetics (AREA)

Abstract

본 발명은 피부 활성물질 탐색용 키트 및 이를 이용하여 피부 활성물질을 탐색하는 방법에 관한 것으로, 보다 상세하게는 장수유전자로 알려진 Klotho 유전자를 포함하는 탐색 키트를 이용하여 피부 세포에서 Klotho 유전자 발현을 유도하는 후보물질을 분리, 정제함으로써 보습, 항노화 물질 및 미백물질 등의 피부 활성물질을 탐색해 내는 피부 활성물질 탐색용 키트 및 이를 이용하여 피부 활성물질을 탐색하는 방법에 관한 것이다.
PCT/KR2011/008132 2010-10-29 2011-10-28 Klotho 유전자를 포함하는 피부 활성물질 탐색용 키트 및 이를 이용하여 피부 활성물질을 탐색하는 방법 WO2012057567A2 (ko)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US13/881,312 US20130252247A1 (en) 2010-10-29 2011-10-28 Kit for screening for skin-activating substances and comprising the klotho gene, and method for screening for skin-activating substances using the same
JP2013536527A JP6186279B2 (ja) 2010-10-29 2011-10-28 Ｋｌｏｔｈｏ遺伝子を含む皮膚活性物質探索用キット及びこれを利用して皮膚活性物質を探索する方法
EP11836659.0A EP2634262B1 (en) 2010-10-29 2011-10-28 Kit for screening for skin-activating substances and comprising the klotho gene, and method for screening for skin-activating substances using same

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20100107000 2010-10-29
KR10-2010-0107000 2010-10-29
KR10-2011-0107409 2011-10-20
KR1020110107409A KR101907567B1 (ko) 2010-10-29 2011-10-20 Ｋｌｏｔｈｏ 유전자를 포함하는 피부 활성물질 탐색용 키트 및 이를 이용하여 피부 활성물질을 탐색하는 방법

Publications (2)

Publication Number Publication Date
WO2012057567A2 WO2012057567A2 (ko) 2012-05-03
WO2012057567A3 true WO2012057567A3 (ko) 2012-09-07

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/008132 WO2012057567A2 (ko) 2010-10-29 2011-10-28 Klotho 유전자를 포함하는 피부 활성물질 탐색용 키트 및 이를 이용하여 피부 활성물질을 탐색하는 방법

Country Status (6)

Country Link
US (1) US20130252247A1 (ko)
EP (1) EP2634262B1 (ko)
JP (1) JP6186279B2 (ko)
KR (1) KR101907567B1 (ko)
CN (1) CN102465182A (ko)
WO (1) WO2012057567A2 (ko)

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KR102045247B1 (ko) * 2013-01-08 2019-11-15 (주)아모레퍼시픽 피부 면역세포를 이용하는 피부 활성물질을 탐색하는 방법 및 이를 이용한 키트
KR102017562B1 (ko) * 2013-03-13 2019-09-03 (주)아모레퍼시픽 미백 효능 물질의 스크리닝 방법
KR102053775B1 (ko) * 2013-07-23 2019-12-09 (주)아모레퍼시픽 미토콘드리아 역동성을 이용한 미백 물질 스크리닝 방법 및 이를 이용한 키트
CN106868089B (zh) * 2015-12-11 2020-02-28 上海家化联合股份有限公司 一种利用皮肤成纤维细胞钾离子通道评价抗衰老功效的方法
KR102692717B1 (ko) * 2016-09-23 2024-08-08 (주)아모레퍼시픽 Tnfrsf14 억제 물질을 포함하는 피부 미백용 조성물 및 tnfrsf14 억제 물질의 스크리닝 방법
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Also Published As

Publication number Publication date
WO2012057567A2 (ko) 2012-05-03
EP2634262A9 (en) 2014-11-19
JP2013541960A (ja) 2013-11-21
EP2634262B1 (en) 2017-05-31
CN102465182A (zh) 2012-05-23
KR20120049126A (ko) 2012-05-16
KR101907567B1 (ko) 2018-10-15
EP2634262A2 (en) 2013-09-04
JP6186279B2 (ja) 2017-08-23
EP2634262A4 (en) 2014-04-16
US20130252247A1 (en) 2013-09-26

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