WO2011116648A1

WO2011116648A1 - Energy-conserving and low-carbon method for culturing ganoderma under industrialized technology

Info

Publication number: WO2011116648A1
Application number: PCT/CN2011/071239
Authority: WO
Inventors: 陈顺志
Original assignee: 江苏学府生物工程有限公司
Priority date: 2010-03-22
Filing date: 2011-02-24
Publication date: 2011-09-29
Also published as: CN101971763A

Abstract

A method for culturing ganoderma by means of ultra-temperature, short time sterilization, combined with quick cooling to room temperature of the nutrient medium of grains such as wheat and maize. Starch pasting and protein denaturation occurs under the high temperature and makes it easy to be absorbed and utilized by the fungus ganoderma, which improves the production efficiency and realizes the purpose of culturing ganoderma under industrialized technology with energy-conserving and low-carbon effect.

Description

Industrialization, energy saving and low carbon cultivation method of Ganoderma lucidum

Technical field:

The invention belongs to the technical field of agricultural technology and traditional Chinese medicine technology, in particular, the cultivation and processing of edible and medicinal fungi.

Background technique:

Edible medicinal fungi are closely related to human health, such as: dozens of fungi such as ganoderma lucidum, white fungus, monkey head, shiitake mushroom and northern cordyceps. A search was conducted in March 2010, and the results showed that: The Chinese Patent Office received as many as 2,562 items of invention patents for Ganoderma lucidum. After years of volatility development, Ganoderma lucidum has evolved from one-way exports to a variety of processed products for internal and external sales. Since the 1960s, Ganoderma lucidum artificial cultivation methods have been mainly based on broad n-trees. Recently, the wheat has become the main component for cultivating Ganoderma lucidum, and a patented invention ZL2004 1 0048020.2 has been obtained. The present invention has been studied on the basis of these, and it has been found that pretreatment is carried out by means of retort sterilization in production, which consumes a large amount of heat and a long length. 100 Ό, atmospheric pressure 8 10 hours; 127 ° C, 1 kg of high pressure also takes 2 hours. In particular, packaging containers such as glass bottles and plastic bottles also raise the temperature. Therefore, the consumption of heat is large and the production time is long. The invention relates to a method for industrializing energy-saving and low-carbon cultivation of ganoderma lucidum, which relates to a method for cultivating edible and medicinal fungi, and achieves the purpose of energy-saving, low-carbon and automatic production through the key technology of expanding the raw material of the culture medium. Comparison of literatures: OM 1075594 relates to the method of manufacturing strains, without involving culture media and cultivation techniques. CN 1162639, CN 1297773, C 1233389 differ from the technical objects, specific methods and meanings of the present invention. C 1444990 and C 1448409 are not involved in culture The composition of the base. C 1099217 is a technical invention for the production of mycelia in 96 hours. CN1387911 is a health wine that improves renal function immunity. 99109683. 5 Name: Ultrasonic puffing technology of Ganoderma lucidum spores, which involves a method of using ultrasonic to puff spores. 200310107651. 2 Name: Puffed Ganoderma lucidum spore powder product and its preparation method, soaking and rinsing Ganoderma lucidum spore powder, drying and tempering at 126~160 °C, puffing and grinding to 20~300 mesh, packaging Into products. 200610067086. 5 Name: The processing method of selenium-enriched puffed food, discloses a processing method of selenium-enriched puffed food. 200710060421. 3 Patent Name: Wheat puffed food and its production method, it is a whole grain wheat puffed food and its production method. It is composed of whole grain wheat, glutinous rice, soybean, mung bean, alfalfa, polygala, walnut kernel, black sesame and seasoning. The whole grain wheat flour is obtained, and the dough is fully mixed and made into a billet, and the billet is sent to a puffing machine for puffing. The puffed semi-finished product is baked in the oven, and the finished product is packaged and stored in the air. 200910065431. 5 ² Name: The production process of raw grain puffed snack food, discloses a production process of raw grain puffed snack food. <Application No.>200510012614 <Invention Name> The puffing treatment process of the edible fungus culture material which is mainly composed of branches and crops, and relates to a puffing treatment process of edible fungus culture materials which are mainly composed of shoots and crop straws The process steps are as follows: (υThe branches and crop straws are pulverized into crumbs or granules or segments; (2) According to the following parts by weight and stirred evenly: Ingredients: including crushed branches or / and crop straws 75-85; organic auxiliary materials: 15 -25; inorganic auxiliary materials: 2-4; (3) puffing treatment: the above-mentioned materials are put into a puffing tank for puffing treatment, the puffing temperature is: 170-210 ° C, The puffing pressure is: 1.2-2.0Mpa, the puffing time is: 10-15 minutes; then the blasting is instantaneously entered into the warehouse; (4) drying: drying the puffed material to a water content of 13-15%, ie Edible mushroom culture Material. By comparison, it is seen that: 1. The culture material of the invention is different from the present invention. 2. The invention needs to enter the drying process after the expansion, and the invention does not need to enter the drying process, and is directly mixed with the bacteria. 3 The strain of the invention is different from the present invention.

The beneficial effects of the invention are that the source of the raw material is widely stable, and the cultivation process of the edible fungus can be simplified, and the bioconversion rate of the edible fungus can be improved. After examining the literature, there is no "method of producing Ganoderma lucidum by using grain as a medium for expansion" which overlaps with the present invention, and puffing and temperature-disinfecting to achieve a culture technique of energy-saving and low-carbon, and shortening the production process. Therefore, the following new invention methods are proposed.

Summary of the invention:

The object of the present invention is to provide a method for puffing wheat, corn and the like as main raw materials, and industrializing energy-saving and low-carbon cultivation of ganoderma lucidum. The medium containing wheat, corn, rice, glutinous rice, millet and yellow rice as the main raw materials is rapidly heated and disinfected by puffing treatment to achieve energy-saving and low-carbon, and shorten the production time. Expanding treatment is a key technology of the present invention out of ^3, generally describes a continuous method of extrusion puffing and air:

1. Continuous extruder: It has the functions of compression, mixing, mixing, melting, puffing and molding. The cavity of the extruder can be divided into 3-5 zones, each zone can be heated by steam or electric, or can be heated by extrusion friction, and the material is gelatinized and denatured by starch under the action of high temperature and high pressure in the cavity. At the end of the extrusion chamber, the molten material is extruded through the die hole of the die plate under the action of high pressure. Due to the sudden drop of pressure, the water vapor rapidly expands and dissipates, causing the product to form a porous structure, and then the puffed material is The rotary knife cuts into a certain size product. The most commonly used in production are single screw extruders and twin screw extruders. This method of temperature in a short cylindrical three increased from between ^ 8 ₀ ° C ~ 140 ° C to 160 ° C, and some puffed cylinder maximum temperature of between 150~190 ° C. Therefore, high temperature can eliminate the medium Bacteria. In addition, starch gelatinization and protein denaturation can be better utilized by Ganoderma lucidum. Therefore, extrusion is the key to the technology in production. From the structure: one shaft or two shafts, called single-twisting machine and 3⁄4嫘杼 extruder; single mast is divided into left-handed and right-handed, twin-screw and in-situ flat double and opposite flat . The characteristics of the material in the barrel are basically the same, but the shear strength and cleaning ability of the material in the same direction are significantly different. The most common are right-handed single-threaded snails and co-directional double twin-screws. From the working mode; according to whether the material enters the extruder barrel (expansion chamber) before steaming and quenching, it is divided into dry extruder and wet extruder. If it is tempered by steam, it is a wet working mode; otherwise, it is a thousand method. The purpose of steam quenching and tempering: to raise the temperature of the material, soften and partially premature the dip, and add some water. For most materials, wet puffing is more efficient than thousand puffing. Therefore, less mechanical energy is required, and more energy can be used to increase production. In addition, due to the softening of the material, the rod of rayon, wear barrel ⁴ also decreases. Due to the dry/wet method, the properties of the materials entering the barrel are different during production. The wet method enters the barrel is a hot and humid soft material. The thousand method enters the barrel is a cold and hard material. Therefore, the configuration requirements for the spindle system and the extrusion screw are also different. In other words, the dry machine can be produced in a wet process, and the wet machine is not necessarily used in a thousand ways.

2, air puffing technology: The initial air puffing equipment is on the street popcorn machine, which is a well-known small business. Every time I process 2 kg of rice, corn or soybeans, children like it very much. At present, air-expansion equipment has been developed, usually with an hourly output: 25-50 kg. There is also a dual air-expansion system with a large output of up to 300 00 kg / h, and has obvious energy-saving effect, a skilled worker can easily operate. The air purifier can puff rice, wheat, corn, buckwheat, beans and other whole grains to maximize the retention The original flavor, color and nutrients of the grain are compared with the traditional airflow tank: easy to operate; lead-free puffing; fast speed; good work performance, stable quality of the produced products. After the air is puffed, the product can maintain the shape of the original material well. The product volume after puffing is the ten letter of the original material volume. The starch in the raw material is essentially 100% ripened. The culture material after puffing is suitable for Ganoderma lucidum growth, and can also be industrialized and automated. Thereby, fresh primordia and fruiting bodies, dried primordia or fruiting bodies, and 100% edible Ganoderma lucidum mycelium (bacteria, ie, slag) are obtained. The invention has a strong applicability and a wide range of applications. In particular, the residual medium (bacteria, slag) of Ganoderma lucidum can directly become a raw material for human food, animal feed, or become a raw material for the production of soy sauce, vinegar, and pharmaceutical products, so that no waste is produced. It resolves the contradiction between the production of Ganoderma lucidum and the ecological balance of forestry, and also provides a new way for the sustainable development of Ganoderma lucidum industry.

The methods and steps are described below:

1. The method for industrializing energy-saving and low-carbon cultivation of Ganoderma lucidum, the invention is characterized in that: wheat, corn as a medium, vitamins and inorganic salts as an additive, temperature-expanding by means of puffing treatment, extrusion granulation, inoculating liquid Ganoderma lucidum Kind, mixed molding, loaded into a container, managed according to known cultivation techniques, the method can be industrialized, automated,

2. The invention is characterized in that: wheat, corn, rice, millet, yellow rice, barley, buckwheat, barley, glutinous rice are used as the medium, and the total content of various foods is 97 100%. Wheat and corn are used as the medium, and the grain components of wheat, corn, rice, millet, yellow rice, barley, buckwheat, barley, and glutinous rice are used as the medium. Wheat accounts for 5-80%, and corn accounts for 5-85%. , glutinous rice accounts for 0-30%; rice, yellow rice, barley, buckwheat, barley can also In the proportion of 0-30%, it becomes the component of the medium; however, the sum of the ingredients of various foods is 97 100%. Other food-based ingredients in the raw materials include bran and rice bran, and the ratio of the raw materials may be 0 - 10% as needed. Nationally approved medicinal and food-based products, such as Codonopsis, Astragalus and kelp, can also be added to improve health outcomes.

3. The invention is characterized in that: the protein, the vitamin and the inorganic salt are used as additives, and the ratio is 0 - 3%. Calcium carbonate, calcium lactate, calcium sulfate, calcium citrate as a regulator, the ratio is 0 - 1%. Calcium can regulate the acidity and alkalinity of the medium and increase the calcium concentration of the product. With reference to the cultivation method of edible fungi, depending on the amount of protein, 0 to 3.0% of bean cake, peanut bean cake, rapeseed cake, and sunflower seed cake can be used. It is also possible to add 0 - 1.0% of nitrogen fertilizer (ammonium citrate and other nitrogenous substances); add 0-0.1% of potassium salt, 0-0.05% of magnesium sulfate. Selenium is an important trace element in the human body. The World Health Organization recommends supplementing 200 selenium every day to prevent high incidence of various diseases. It supports the use of safer selenium-containing proteins, amino acids and other organic forms of selenium, or selenium-rich agricultural by-products. Currently, sodium selenite tablets contain 1 mg of sodium selenite per tablet (equivalent to 457 g of selenium) over 11 years of age and 2 tablets per adult. Taking too much selenium can cause poisoning. The maximum daily safe dose is 400 ug _0. Therefore, refer to this data to add trace element selenium in the medium at a ratio of 0 -0. 8 mg / kg. Allows the finished product to reach 20-50 g per 100 g of Ganoderma Lucidum. Vitamins are lost during the expansion process. Therefore, a small amount of vitamin B is usually added. Alternatively, the proportion of yeast added is 0-0.5%. The yeast contains a large amount of vitamin B group components. Because trace elements and vitamins differ in raw materials, they range from 0% to 0.5%, depending on the source of the raw materials. Line considerations. These additives for protein, vitamins and inorganic salts have two methods of addition in the production process: 1. After the additives of protein, vitamins and inorganic salts are crushed, they are thoroughly mixed with the raw materials of the grain, and then puffed to achieve disinfection; After pulverizing the additives of protein, vitamins and inorganic salts, it is dissolved in water more than ten times, autoclaved, mixed with liquid Ganoderma lucidum strain, and used in the stirring process.

4. The invention is characterized in that: the temperature is sterilized by the puffing treatment, and the medium raw material is formed into a porous structure, the temperature of the puffing production is 150 - 210 ° C, and the optimum temperature is 150-170 ° C. The simplest puffing technique is a method of screening, washing, drying, and then frying it into fried rice. Production methods using a continuous process are: continuous extrusion puffing and air puffing techniques.

A. Continuous extruder: It has the functions of compression, mixing, mixing, melting, puffing and forming ₇ . The product is formed into a porous structure, and then the expanded material is cut into a certain size product by a rotary knife. The most commonly used in production are single screw extruders and twin screw extruders. In this way, the temperature of the three cylinders is increased from 80 ° C to 140 ° C to 160 ° C in a short time, and some of the expansion cylinders have a maximum temperature of 150 to 210 ° C. Therefore, high temperatures can destroy bacteria in the medium. In addition, starch gelatinization and protein denaturation can be better utilized by Ganoderma lucidum. Therefore, extrusion is the key to the technology in production. From the structure: a shaft or two shafts, called single-screw extruder and twin-screw extruder; the most common is the right-handed single mast and the same-direction double twin-screw. From the working mode: According to the material before entering the extruder barrel (expansion chamber), it is divided into dry extruder and wet extruder by steam quenching and tempering. If it is tempered by steam, it is in wet mode; otherwise, it is dry. The purpose of steam quenching and tempering: to raise the temperature of the material, soften and partially pre-mature the material, and add some water. For most materials, wet expansion is more than one thousand The expansion efficiency is high. Therefore, less mechanical energy is required, and more energy can be used to increase production. Outside A, the wear on the screw and barrel is also reduced due to the softening of the material. Due to the dry/wet method, the properties of the material entering the barrel are different during production. The wet method is a hot and humid material, and the dry method enters the barrel as a cold and hard material. Therefore, the configuration requirements for the spindle system and the extrusion screw are also different. In other words, the dry machine can be produced wet, while the wet machine is not necessarily dry. Both the dry and wet machines of the present invention can be used; the optimum temperature for extrusion extruder production is 150 170 °C.

B. Air-expansion technology: At present, the air-expansion equipment developed has an output of 25-50 kg per hour. There is also a dual airflow puffing system with a larger output of up to 300 500 kg/h, which is 1/3 more energy efficient than conventional models. The temperature inside the tank reached 200 ° C, and the volume of the grain raw material increased by 10 times. The higher the temperature, the larger the expansion volume. In the production of the present invention, the volume expansion of the ₈ raw materials is increased, which is not the ultimate purpose and effect. What is needed is to destroy the bacteria, and the appropriate expansion volume is 3-5 times to achieve the purpose. Now, the new device uses a temperature sensing system that instantly reflects the correct temperature inside the tank. Microorganisms can be killed in a few seconds due to 150-180 °C. Therefore, according to the temperature indication in production, as long as the sterilization requirements are met. The airflow puffing working range is 150-200 °C, and the process of the invention does not require 200 ° C high temperature, and can achieve energy saving and low carbon. The optimum temperature for the technique of the present invention is 150-170 °C.

5. The invention is characterized in that: extrusion granulation is carried out, and the extruded material has a rice grain shape, a square shape, a circular shape, a rectangular shape, a rhombus shape and other geometric shapes or an air flow puffing to maintain the original shape of the grain particles. The purpose of the present invention to form a shape is to allow the culture material to be thoroughly mixed and shaped with the liquid Ganoderma lucidum strain. The culture material after treatment with air-expansion technology, such as wheat, The corn is kept in its original shape, and the granular wheat and corn culture can be thoroughly mixed with the liquid Ganoderma lucidum. The particles after extrusion molding are beneficial to the growth and development of Ganoderma lucidum.

6. The present invention is characterized in that the cooling process after the puffing treatment is such that the temperature reaches 25 or less. The temperature of wheat and corn culture materials after the puffing technology treatment is relatively high. Liquid Ganoderma lucidum is a living life and is not suitable for temperatures above 25 °C. Therefore, a cooling process is required to bring the temperature below 25 °C. The material of the twin-screw extruder is 8-10 seconds in the high temperature pipeline to achieve the disinfection effect. Its water cooling system reduces the temperature of the material to around 85 °C, which is beneficial for production applications under GMP conditions. The present invention uses a wind cooling technique, or a natural cooling technique, to bring the temperature to 25 ° C or lower and then inoculate the Cordyceps militaris.

7. The invention is characterized in that: the liquid Ganoderma lucidum strain is inoculated, the ratio of the culture material to the liquid is 100: 100-500, and the most suitable range is: Culture material: liquid = 100: 110 - 300. ⁹ The method for obtaining the liquid Ganoderma lucidum strain is: The liquid Ganoderma lucidum strain is produced according to a known conventional method, and a vitamin, an inorganic salt additive, and a liquid composed of water (after autoclaving) may be added during use. It is also possible to use a solid Ganoderma lucidum strain under GMP conditions or a sterile room aseptic requirements, add 2-5 volumes of sterile liquid (water), and use a blender and a homogenizer to break into a liquid Ganoderma lucidum strain. Mix well with liquid Ganoderma lucidum strain and expanded culture. Previous cultures were moist after autoclaving or 8 hours of cooking, with a water content of 80-150%. The water content of the expanded culture material is 6-18%, and the amount of liquid used in the method is relatively large; this is completely different from the conventional method. The liquid can be replenished with sterile water or physiological saline. Sterile water and physiological saline can be obtained by ordinary sterilization methods or filtration techniques. 8. The invention is characterized in that: the culture material and the liquid Ganoderma lucidum strain are mixed into a square shape, a barrel shape, a rectangular shape, a diamond shape and other geometric shapes, and are loaded into the container. Molding methods are either manual or semi-mechanical filling, or production by assembly line. The purpose of making a shape is to allow the culture material to be thoroughly mixed with the liquid Ganoderma lucidum strain, usually by electric stirring in a stirred tank. According to the conventional method, the container is a plastic bag (box, bottle, stick, box) container, a paper material container, a paper plastic recyclable material container or a glass container for manual production, or a semi-mechanical filling, or a production line. After the culture material and the stirring tank are completed, the sleeve molding process for the production line in the molding production process is as follows: The container is first introduced into the trough, refilled, rolled, grooved, capped, put on, cultivated, and shed. Or the method of using compression molding is as follows: After the seed is mixed, it is molded into a block, then placed in a container, capped, racked, incubated, and grassed. The former container is first placed in the tank, and then filled and capped; the latter is pressed into a block and then placed in a container and capped. Therefore, the features of the present invention are: a sleeve molding process for forming a molding line or a method of compression molding. In order to be sold in a supermarket, a container can be bagged, a mixture of the culture material and the Ganoderma lucidum strain is grown in the bag, and after maturing, the customer takes the finished product in the bag. The role of the container is to maintain growth space, easy to sell and transport in the supermarket. Here the container can be reused to achieve energy-saving and low-carbon effects. There is a certain amount of growth space in this container. It is also possible to leave no growth space in the container. After the mature fruit body grows out of the surface, it is covered with another plastic bag (box, bottle, box) container, paper material container, paper plastic recyclable material container or glass container. A method of performing growth management of a fruiting body.

9. After the above steps, it can be managed according to known cultivation techniques. This method can automate production management. Automated production management is reflected in: preparation, expansion process, cooling, inoculation, sub-assembly, transport to the culture room, culture room temperature, humidity, illuminance Management, harvesting process, can be mechanized or automated production.

10. The characteristics of the present invention are: the species of Ganoderma lucidum involved, the species cultivated by the Ganoderma lucidum strain preserved by the National Center of the Species as a new method (Ganoderma lucidum species preserved in the Center of the Species before the patent application date); Mainly include: Ganoderma lucidum, southern tree tongue, Minnan Ganoderma lucidum, Guizhou Ganoderma lucidum, layered tongue, sessile Ganoderma lucidum, Sanming tree tongue, Zizhi, tropical Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Bombyx chinensis, Huang Lingzhi, Ganoderma lucidum , Shandong Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma Lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum , antler Ganoderma lucidum, Antrodia camphorata, using the above strains to make liquid strains inoculated in puffed grain culture materials to complete new technologies and new methods. The method of liquid bacteria has been described above. The use of the new method has the following advantages: The invention has the following principles: "Industrial energy-saving and low-carbon cultivation of Ganoderma lucidum method ¹¹ ", the characteristics are as follows: 1. Using the principle that the raw material of the grain can be expanded, rapidly heating and disinfecting, achieving the purpose of energy saving and low carbon, and shortening the production time 2, glass bottles, plastic bottles and other packaging containers do not need to heat up, can save energy and low carbon; 3, can cultivate Ganoderma lucidum on the assembly line, in order to reduce costs, implement automated production, opened up a broad prospect; 4, can achieve Ganoderma lucidum And the whole process of quality control of active ingredient processing has laid a solid foundation for Chinese medicine products to enter the international market.

The following is an example:

Example 1: Using 20 kg of wheat, 75 kg of corn, 5 kg of rice bran, and then adding 0.3 kg of ammonium citrate, 0.1 kg of potassium salt, 0.05 kg of magnesium sulfate, 0.5 kg of calcium carbonate, after twin-screwing Extruding at 150~170 °C, Rice grain culture material. The material of the 3⁄4 mast extrusion extruder is 8-10 seconds in the high temperature pipeline to achieve the disinfection effect. Its water cooling system reduces the temperature of the material to around 85 °C. Cool under GMP conditions, make the temperature below 25 °C, mix well with liquid Cordyceps sinensis, fill the plastic water container and glass bottle with 100: 150 water, and add 0.5 kg mixed culture to each container. Material, seal. After the surface of the culture material is covered with hyphae, the ventilation is increased. Managed according to known cultivation techniques. Thereby, a fruit body can be obtained, and a fresh primordium of a semicircular shape, an elliptical shape, a dendritic shape or a spherical shape can be collected to become a Ganoderma lucidum commodity. After collecting fresh Ganoderma lucidum primordia or fruiting body, the medium portion is an edible Ganoderma lucidum mycelium (ie, bacillus, slag).

Example 2: 25 kg of wheat, 50 kg of corn, 15 kg of glutinous rice, 3 kg of rice bran, 2 kg of bran, and then added 0.3 kg of ammonium citrate, 0.1 kg of potassium salt, 0.05 kg of magnesium sulfate, 0.5 after kg of calcium carbonate were mixed gas ¹² flows through 150- 17CTC expanded, puffed original shape resulting culture medium. Cool to inoculate the temperature below 25 。. It is well mixed with liquid Ganoderma lucidum strains, and the liquid Ganoderma lucidum strain contains added vitamin B and yeast. The ratio of material to water is 100: 180, mechanically filled in a plastic container through a production line, and 0.22 kg of mixed culture material is added to each square container to seal. The growth space is not retained in the container, and after the mycelium grows over the surface, it can be transported to another city over a long distance. Use another plastic bag (box, bottle, box) container, paper material container, paper plastic recyclable material container or glass container. Harvest the product according to known cultivation techniques.

Example 3: 20 kg of wheat, 65 kg of corn, 10 kg of glutinous rice, 3 kg of rice bran, 0.2 kg of kelp crushed, and then added 0.3 kg of ammonium citrate, 0.1 kg of potassium salt, 0.05 kg of magnesium sulfate, 0.5克的碳酸元素硒。 0.5 kg of calcium carbonate and 0.6 g of trace element selenium. After mixing, the mixture was extruded by a double mast extruder to obtain a pellet-shaped culture material. Cool, and inoculate the temperature below 25 °C. Fully mixed with liquid Ganoderma lucidum strain, water ratio 100: 120, the molding process in the molding production process is: The container first enters the trough, refills, rolls forming, troughing, capping, adding 0.5 to each container Kilograms of mixed culture, sealing, racking, cultivating, and grassing. In order to be sold in a supermarket, a container can be bagged, a mixture of the culture material and the Ganoderma lucidum strain is grown in the bag, and after maturing, the customer takes the finished product in the bag.

Example 4:

Using 20 kg of wheat and 80 kg of corn, a pellet-shaped culture material was obtained through a flow expansion apparatus at 150-160 °C. Cool to inoculate the temperature below 25 °C. 0.3 g of ammonium citrate, 0.1 kg of potassium salt, 0.05 kg of magnesium sulfate, added to the yeast 0.5 kg, mixed and dissolved in 10 kg of water, autoclaved, mixed with liquid Ganoderma lucidum strain, stir the Ganoderma lucidum The bacteria are mixed thoroughly to make the ratio of water to water 100: 180; if the liquid Ganoderma lucidum strain ¹³ and the inorganic salt and vitamin water are not enough 180 kg, it can be supplemented with sterile water or physiological saline. Sterile water and physiological saline can be obtained by ordinary sterilization methods or filtration techniques. After stirring, the containers were filled in a square plastic bottle container, and 1 kg of the mixed culture material was added to each container. The method of compression molding is as follows: After the seed is mixed, it is molded into a block, then placed in a container, capped, racked, incubated, and managed according to known cultivation techniques.

references:

1. Lin Zhibin: Modern Research on Ganoderma Lucidum, Beijing Medical University Press, March 2001 2nd Edition 94, 157, 310

2. Lin Shuqian: Production and Development of Chinese Medicinal Bacteria, China Agricultural Press, November 2000, 1st edition 211, 257

Claims

Claim

1. The method for industrializing energy-saving and low-carbon cultivation of Ganoderma lucidum, the invention is characterized in that: wheat, corn as a medium, vitamins and inorganic salts as additives, temperature-expanding by puffing treatment, extrusion granulation, inoculating liquid Ganoderma lucidum Kind, mixed molding, loaded into a container, managed according to known cultivation techniques, the method can be industrialized, automated,

2. The method according to claim 1, characterized in that the wheat, corn, rice, millet, yellow rice, barley, buckwheat, barley, glutinous rice are used as a medium, and the sum of various food components is 97. 100%.

The method according to claim 1, characterized in that the protein, the vitamin and the inorganic salt are used as additives, and the ratio is from 0 to 3%.

The method according to claim 1, characterized in that: the temperature-increasing sterilization is carried out by the expansion treatment to form a porous structure of the medium, and the temperature of the expansion is 150 - 210 ° C, and the optimum temperature is 150 - 170 Ό.

The method according to claim 1, characterized in that: the extrusion granulation is carried out, and the extruded material has a rice grain shape, a square shape, a circular shape, a rectangular shape, a rhombus shape and other geometric shapes or an air flow puffing to maintain the grain granules. Original shape.

The method according to claim 1, wherein the cooling process after the puffing treatment is such that the temperature reaches 25 ° C or lower.

The method according to claim 1, characterized in that: the liquid Ganoderma lucidum strain is inoculated, and the ratio of the culture material to the liquid is 100: 100-500, and the most suitable range is: Nourishment: Liquid = 100: 110 - 300.

8. The method of claim 1 wherein: the culture material and the liquid ganoderma species are mixed into a square, a barrel, a rectangle, a diamond, and other geometric shapes and loaded into the container.

9. The method of claim 1 wherein: the method of forming a sleeve or stamper for forming a line.

The method according to claim 1, characterized in that: the present invention is characterized in that: the species of Ganoderma lucidum involved, a species cultivated by a Ganoderma lucidum strain preserved at the National Center for Strain, as a new method (patent application) Ganoderma lucidum strains preserved in the center of the species before the day; mainly include: Ganoderma lucidum, southern tree tongue, Ganoderma lucidum, Guizhou Ganoderma lucidum, layered tongue, sessile Ganoderma lucidum, Sanming tree tongue, Zizhi, tropical Ganoderma lucidum, pine Sushen Ganoderma lucidum, Black Ganoderma lucidum, Botanic lucidum, Huang Lingzhi, Ganoderma lucidum, Shandong Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum Ganoderma Lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, Ganoderma lucidum, and new techniques and methods for inoculating liquid cultures in puffed food culture materials.