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WO2000031294A3 - Verfahren zur identifikation von cytosin-methylierungsmustern in genomischer dna - Google Patents

Verfahren zur identifikation von cytosin-methylierungsmustern in genomischer dna Download PDF

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Publication number
WO2000031294A3
WO2000031294A3 PCT/DE1999/003747 DE9903747W WO0031294A3 WO 2000031294 A3 WO2000031294 A3 WO 2000031294A3 DE 9903747 W DE9903747 W DE 9903747W WO 0031294 A3 WO0031294 A3 WO 0031294A3
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WO
WIPO (PCT)
Prior art keywords
genomic dna
steps
cell
identifying
heteroduplexes
Prior art date
Application number
PCT/DE1999/003747
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English (en)
French (fr)
Other versions
WO2000031294A2 (de
Inventor
Kathrin Berlin
Original Assignee
Epigenomics Gmbh
Kathrin Berlin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Gmbh, Kathrin Berlin filed Critical Epigenomics Gmbh
Priority to CA002351143A priority Critical patent/CA2351143A1/en
Priority to JP2000584101A priority patent/JP2002530118A/ja
Priority to EP99962069A priority patent/EP1135526A2/de
Priority to NZ511638A priority patent/NZ511638A/en
Priority to AU18565/00A priority patent/AU757473B2/en
Priority to IL14317699A priority patent/IL143176A/xx
Publication of WO2000031294A2 publication Critical patent/WO2000031294A2/de
Publication of WO2000031294A3 publication Critical patent/WO2000031294A3/de
Priority to IS5916A priority patent/IS5916A/is

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Beschrieben ist ein Verfahren zur Identifikation von 5-Methylcytosin-Positionen in genomischer DNA, welches dadurch gekennzeichnet ist, daß folgende Verfahrensschritte ausgeführt werden: a) die genomische DNA einer Zelle, einer Zellinie, eines Gewebes oder eines Individuums wird chemisch so behandelt, daß Cytosin und 5-Methylcytosin unterschiedlich reagieren und sich in der Duplex ein unterschiedliches Basenpaarungsverhalten der beiden Produkte ergibt, b) derselbe Nukleinsäure-Abschnitt mittels einer Polymerasereaktion amplifiziert wird, c) der gleiche Nukleinsäure-Abschnitt mindestens einer weiteren Zelle, Zellinie, Gewebes oder Individuums oder einer beliebigen Referenz-DNA entsprechend den Punkten a) und b) behandelt wird, d) aus den mindestens zwei Amplifikaten der Punkte b) und c) Heteroduplexes gebildet werden, e) durch eine Reaktion, die spezifisch ist für nicht komplementäre Basenpaare, eine aufzeigbare Markierung in die Heteroduplex eingeführt wird.
PCT/DE1999/003747 1998-11-19 1999-11-19 Verfahren zur identifikation von cytosin-methylierungsmustern in genomischer dna WO2000031294A2 (de)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CA002351143A CA2351143A1 (en) 1998-11-19 1999-11-19 Method for identifying cytosine methylation patterns in genomic dna
JP2000584101A JP2002530118A (ja) 1998-11-19 1999-11-19 ゲノムｄｎａにおけるシトシンのメチル化パターン同定法
EP99962069A EP1135526A2 (de) 1998-11-19 1999-11-19 Verfahren zur identifikation von cytosin-methylierungsmustern in genomischer dna
NZ511638A NZ511638A (en) 1998-11-19 1999-11-19 Method for identifying cytosine methylation patterns in genomic DNA
AU18565/00A AU757473B2 (en) 1998-11-19 1999-11-19 Method for identifying cytosine methylation patterns in genomic DNA
IL14317699A IL143176A (en) 1998-11-19 1999-11-19 Method for identifying cytosine methylation patterns in genomic dna
IS5916A IS5916A (is) 1998-11-19 2001-04-10 Aðferð til að auðkenna sýtósín metýleringarmynstur í gena DNA

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19853398.5 1998-11-19
DE19853398A DE19853398C1 (de) 1998-11-19 1998-11-19 Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischer DNA

Publications (2)

Publication Number Publication Date
WO2000031294A2 WO2000031294A2 (de) 2000-06-02
WO2000031294A3 true WO2000031294A3 (de) 2000-10-19

Family

ID=7888355

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1999/003747 WO2000031294A2 (de) 1998-11-19 1999-11-19 Verfahren zur identifikation von cytosin-methylierungsmustern in genomischer dna

Country Status (9)

Country Link
EP (1) EP1135526A2 (de)
JP (1) JP2002530118A (de)
AU (1) AU757473B2 (de)
CA (1) CA2351143A1 (de)
DE (1) DE19853398C1 (de)
IL (1) IL143176A (de)
IS (1) IS5916A (de)
NZ (1) NZ511638A (de)
WO (1) WO2000031294A2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9929720D0 (en) * 1999-12-17 2000-02-09 Zeneca Ltd Diagnostic method
DE10032529A1 (de) * 2000-06-30 2002-02-07 Epigenomics Ag Diagnose von bedeutenden genetischen Parametern innerhalb des Major Histocompatibility Complex (MHC)
DE10019058A1 (de) * 2000-04-06 2001-12-20 Epigenomics Ag Detektion von Variationen des DNA-Methylierungsprofils
EP1276860B1 (de) 2000-04-28 2007-09-26 Sangamo Biosciences Inc. Datenbanken von regulatorischen sequenzen, methoden zu ihrer herstellung und verwendung
DE10029915B4 (de) * 2000-06-19 2005-06-16 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
IL154663A0 (en) * 2000-09-01 2003-09-17 Epigenomics Ag Method for determining the degree of methylation of defined cytosines in genomic dna in the sequence context 5'-cpg-3'
DE10050942B4 (de) * 2000-10-10 2005-11-17 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
GB0025913D0 (en) 2000-10-23 2000-12-06 Guldberg Per Materials and methods relating to nucleic acid amplification and profiling
WO2002034942A2 (en) * 2000-10-23 2002-05-02 Cancer Research Technology Limited Nucleic acid amplification-based methods for the determination of a methylation profile and reagents therefor
DE10061348C2 (de) * 2000-12-06 2002-10-24 Epigenomics Ag Verfahren zur Quantifizierung von Cytosin-Methylierungen in komplex amplifizierter genomischer DNA
DE10132212B4 (de) * 2001-06-27 2005-11-24 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung durch vergleichende Analyse der Einzelstränge von Amplifikaten
US6884586B2 (en) 2001-07-15 2005-04-26 Keck Graduate Institute Methylation analysis using nicking agents
JP2003144172A (ja) * 2001-11-16 2003-05-20 Nisshinbo Ind Inc メチル化検出用オリゴヌクレオチド固定化基板
AU2003212878A1 (en) * 2002-01-30 2003-09-02 Epigenomics Ag Identification of cell differentiation states based on methylation patterns
DE10204566A1 (de) * 2002-02-04 2003-08-14 Nanogen Recognomics Gmbh Verfahren zur Bestimmung des Methylierungsmusters von DNA
DE102004002257B4 (de) * 2004-01-09 2006-11-09 Epigenomics Ag Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA mit Hilfe von DNA-Reparaturenzymen
WO2006132022A1 (ja) * 2005-06-08 2006-12-14 Kyoto University メチルシトシンの簡便検出法
US20100167942A1 (en) * 2008-12-23 2010-07-01 New England Biolabs, Inc. Compositions, Methods and Related Uses for Cleaving Modified DNA

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WO1993022457A1 (en) * 1992-04-24 1993-11-11 Massachusetts Institute Of Technology Screening for genetic variation
WO1997033000A1 (en) * 1996-03-04 1997-09-12 Genetrace Systems, Inc. Methods of screening nucleic acids using mass spectrometry
WO1997045560A1 (en) * 1996-05-31 1997-12-04 North Shore University Hospital Research Corporation Identification of differentially methylated and mutated nucleic acids
WO1997046705A1 (en) * 1996-06-03 1997-12-11 The Johns Hopkins University School Of Medicine Methylation specific detection
WO1999028498A2 (de) * 1997-11-27 1999-06-10 Epigenomics Gmbh Verfahren zur herstellung komplexer dna-methylierungs-fingerabdrücke

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DD293139A5 (de) * 1989-06-29 1991-08-22 Humboldt-Universitaet Zu Berlin,Direktorat Fuer Forschung,De Verfahren zur charakterisierung bestimmter dna-sequenzen
US5824471A (en) * 1995-06-05 1998-10-20 Brigham And Women's Hospital Detection of mismatches by cleavage of nucleic acid heteroduplexes

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993002216A1 (en) * 1991-07-19 1993-02-04 Upstate Biotechnology, Inc. Method for detection of mutations
WO1993022457A1 (en) * 1992-04-24 1993-11-11 Massachusetts Institute Of Technology Screening for genetic variation
WO1997033000A1 (en) * 1996-03-04 1997-09-12 Genetrace Systems, Inc. Methods of screening nucleic acids using mass spectrometry
WO1997045560A1 (en) * 1996-05-31 1997-12-04 North Shore University Hospital Research Corporation Identification of differentially methylated and mutated nucleic acids
WO1997046705A1 (en) * 1996-06-03 1997-12-11 The Johns Hopkins University School Of Medicine Methylation specific detection
WO1999028498A2 (de) * 1997-11-27 1999-06-10 Epigenomics Gmbh Verfahren zur herstellung komplexer dna-methylierungs-fingerabdrücke

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Title
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WARNECKE ET AL.: "DETECTION AND MEASUREMENT OF PCR BIAS IN QUANTITATIVE METHYLATION ANALYSIS OF BISULPHITE-TREATED DNA", NUCLEIC ACIDS RESEARCH, vol. 25, no. 21, 1997, pages 4422 - 4426, XP002138268 *
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ZESCHNIGH M ET AL: "Imprinted segments in the human genome: different DNA methylation patterns in the Prader-Willi/Angelman syndrome region as determined by the genomic sequencing method", HUMAN MOLECULAR GENETICS,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 6, no. 3, 1997, pages 387 - 395-395, XP002106412, ISSN: 0964-6906 *

Also Published As

Publication number Publication date
NZ511638A (en) 2003-11-28
DE19853398C1 (de) 2000-03-16
AU757473B2 (en) 2003-02-20
AU1856500A (en) 2000-06-13
EP1135526A2 (de) 2001-09-26
CA2351143A1 (en) 2000-06-02
IS5916A (is) 2001-04-10
WO2000031294A2 (de) 2000-06-02
JP2002530118A (ja) 2002-09-17
IL143176A (en) 2005-05-17
IL143176A0 (en) 2002-04-21

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