US20040096932A1

US20040096932A1 - Enzyme catalysis in the presence of ionic liquids

Info

Publication number: US20040096932A1
Application number: US10/416,067
Authority: US
Inventors: Udo Kragl; Nicole Kaftzik; Sonja Schofer; Peter Wasserscheid
Original assignee: Individual
Current assignee: Individual
Priority date: 2000-11-08
Filing date: 2001-11-07
Publication date: 2004-05-20
Also published as: EP1332221A1; DE50110361D1; WO2002038784B1; US20080299623A1; JP4195610B2; ATE331803T1; AU2002220685A1; US7754462B2; WO2002038784A1; US20060211096A1; JP2004513639A; EP1332221B1; EP1205555A1; ES2267856T3

Abstract

The invention relates to the implementation of enzyme-catalysed reactions in the presence of ionic liquids.

Description

The invention relates to compositions comprising an enzyme and ionic liquids as well as a method for carrying out enzyme-catalysed reactions in the presence of ionic liquids.

To date, enzymes have become firmly established as biocatalysts for reactions on the laboratory and industrial scale. Nevertheless, despite all the success with enzymatic reactions, there are still problems such as, for example,

low productivities as a result of too low educt solubilities;

low yields in equilibrium reactions;

insufficient selectivity in regio- and stereoselective conversions;

product inhibition;

the occurrence of side reactions (parallel, consecutive reactions).

There are known attempts to solve this problem by adding organic solvents (G. Carrea, S. Riva, Angew, Chem. 2000, 112, 2312; J. M. S. Cabral, M. R. Aires-Barros, H. Pinheiro, D. M. F. Prazeres, J. Biotechnol. 1997, 59, 133; M. N. Gupta, Eur. J. Riochem. 1992, 203, 25), by adding salts (A. M. Blinkorsky, Y. L. Khmelnitzky, J. S. Dordick, J. AM, Chem. Soc. 1999, 116, 2697) or by carrying out the reaction in microemulsions (B. Orlich, R. Schomähcker 1999, 65, 357-362). Frequently however, the improvements achieved thereby are not significant and do not justify the additional expenditure, or the enzyme stability decreases severely under these conditions (G. Carrea, S. Riva, Angew. Chem. 2000, 112, 2312). At low temperatures (<100° C.) ionic liquids are melting salts which represent a new class of solvents having a non-molecular ionic character. Although the first representatives have been known since 1914, ionic liquids have only been investigated intensively as solvents for chemical conversions in the last 15 years. Ionic liquids have no measurable vapour pressure. This is a major advantage from the process engineering point of view because in this way, the distillative separation of a reaction mixture is possible as an effective method for product separation. The known problems caused by azeotrope formation between solvents and products do not occur. Ionic liquids are temperature-stable up to above 200° C. By means of a suitable choice of cation and anion, it is possible to gradually adjust the polarity and thereby tune the solubility properties. The range goes from water-miscible ionic liquids through water-immiscible ionic liquids as far as those which themselves form two phases with organic solvents. The skilful utilization of the extraordinary solubility properties is the key to the successful use of ionic liquids as a new class of solvents.

Ionic liquids have already been successfully used as new types of media in two-phase catalysis or as the medium for liquid-liquid extraction). (P. Wasserscheid, W. Keim, Angew. Chem. 2000, 112, 3926).

According to the invention a substantial increase in the yield and selectivity was surprisingly established during the conversion of a wide range of educts with different enzymes in the presence of ionic liquids, which represents a significant improvement compared with the prior art. No adverse effects of the ionic liquid on the enzyme stability were established and in individual cases, even a stabilising effect was found.

This is unexpected and surprising bearing in mind th ionic nature of the ionic liquids and the strong interactions thereby possible between the ionic liquids and the enzyme with its likewise charged groups.

It was also found that ionic liquids can be used as co-solvents to improve the solubility of educts and products.

The invention relates to a method for the conversion of substances (educts) in the presence of enzymes as a catalyst in a reaction medium comprising ionic liquids.

The ionic liquid can be miscible with water or immiscible with water. In the same way, it is possible to carry out a single-phase, two-phase or multi-phase reaction.

The ionic liquids comprise compounds having the general formula

[A]_n ⁺[Y]ⁿ⁻,

where

n 1 or 2 and

the anion [Y] ⁿ⁻ is selected from the group comprising tetrafluoroborate ([BF₄]⁻), tetrachloroborate ([BCl₄]⁻), hexafluorophosphate ([PF₆]⁻), hexafluoroantimonate ([SbF₆]⁻), hexafluoroarsenate ([AsF₆]⁻), tetrachloroaluminate ([AlCl₄]⁻), trichlorozincate [(ZnCl₃]⁻), dichlorocuprate, sulphate ([SO₄]²⁻), carbonate ([CO₃]²⁻), fluorosulphonate, [R′—COO]⁻, [R′—SO₃]⁻ or [(R′—SO₂)₂N]⁻, and R′ is a linear or branched aliphatic or alicyclic alkyl containing 1 to 12 carbon atoms or a C₅-C₁₈-aryl, C₅-C₁₈-aryl-C₁-C₆-alkyl or C₁-C₆-alkyl-C₅-C₁₈-aryl radical that can be substituted by halogen atoms, the cation [A]⁺ is selected from

quaternary ammonium cations having the general formula

[NR¹R²R³R]⁺,

phosphonium cations having the general formula

[PR¹R²R³R]⁺,

imidazolium cations having the general formula

where the imidizole nucleus can be substituted with at least one group selected from C ₁-C₆-alkyl, C₁-C₆-alkoxy, C₁-C₆-aminoalkyl, C₅-C₁₂-aryl or C₅-C₁₂-aryl-C₁-C₆-alkyl groups,

pyridinium cations having the general formula

where the pyridine nucleus can be substituted with at least one group selected from C ₁-C₆-alkyl, C₁-C₆-alkoxy, C₁-C₆-aminoalkyl, C₅-C₁₂-aryl or C₅-C₁₂-aryl-C₁-C₆-alkyl groups,

pyrazolium cations having the general formula

where the pyrazole nucleus can be substituted with at least one group selected from C ₁-C₆-alkyl, C₁-C₆-alkoxy, C₁-C₆-aminoalkyl, C₆-C₁₂-aryl or C₅-C₁₂-aryl-C₁-C₆-alkyl groups,

and triazolium cations having the general formula

where the triazole nucleus can be substituted with at least one group selected from C ₁-C₆-alkyl, C₁-C₆-alkoxy, C₁-C₆-aminoalkyl, C₅-C₁₂-aryl or C₅-C₁₂-aryl-C₁-C₆-alkyl groups,

and the radicals R ¹, R², R³are selected independently of one another from the group consisting of

hydrogen;

linear or branched, saturated or unsaturated, aliphatic or alicyclic alkyl groups with 1 to 20 carbon atoms;

heteroaryl, heteroaryl-C ₁-C₆-alkyl groups with 3 to 8 carbon atoms in the heteroaryl radical and at least one heteroatom selected from N, O and S which can be substituted with at least one group selected from C₁-C₆-alkyl groups and/or halogen atoms;

aryl-, aryl-C ₁-C₆-alkyl groups with 5 to 12 carbon atoms in the aryl radical which if necessary can be substituted with at least one C₁-C₆-alkyl group and/or one halogen atom.

In a further aspect the invention relates to a composition comprising an enzyme and at least one of the ionic liquids defined above. These compositions can be used as the starting point for carrying out the afore-mentioned enzymatically catalysed reactions. Accordingly, in addition to the enzyme (biocatalyst), the compositions according to the invention can also contain the educts (substrate) to be converted and as the reaction proceeds, naturally also the reaction products obtainable by the enzymatic reaction.

A still further aspect is thus the use of ionic liquids, especially the ionic liquids defined above, as the reaction medium or a constituent of the reaction medium in biocatalysis, i.e., carrying out enzymatically catalysed reactions on substrates.

In a particular development of the invention the alkyl, aryl, arylalkyl and alkylaryl sulphonate groups (anion [Y]) can be substituted by halogen atoms, especially fluorine, chlorine or bromine. Especially preferred are the perfluorinated alkyl and afore-mentioned aryl sulphonates such as trifluoromethane sulphonate (triflate). As non-halogenated representatives mention may be made of methane sulphonate, benzene sulphonate and the toluene sulphonate group as well as other sulphonate leaving groups known in the prior art.

In a further development of the invention the alkyl, aryl, arylalkyl and alkylaryl carboxylate groups can be substituted by halogen atoms, especially fluorine, chlorine or bromine, Especially preferred are the fluorinated, in particular the perfluorinated alkyl and above-mentioned aryl carboxylates, such as trifluoromethane carboxylate (trifluoroacetate; CF ₃COO⁻). As non-halogenated representatives mention may be made of the acetate and benzoate group as well as all other carboxylate leaving groups known in the prior art.

In preferred developments of the invention the C ₁-C₆-alkyl groups mentioned in connection with the substituents can be replaced by C₂-C₄-alkyl groups independently of each other. Likewise, the C₁-C₆-alkoxy groups mentioned in connection with the substituents can be replaced by C₂-C₄-alkoxy groups independently of each other. In a further alternative of the invention the C₅-C₁₂-aryl groups mentioned in connection with the substituents can be replaced by C₆-C₁₀-aryl groups independently of each other and the C₃-C₈-heteroaryl groups can be replaced by C₃-C₆-heteroaryl groups independently of one another. The halogen atoms with which the alkyl, alkoxy and aryl groups can be substituted are selected from fluorine, chlorine, bromine and iodine, preferably fluorine, chlorine and bromine.

In a preferred development the radical R′ is a linear or branched aliphatic or alicyclic alkyl containing 1 to 8 carbon atoms or a C ₆-C₁₀-aryl, C₆-C₁₀-aryl-C₁-C₄-alkyl or C₁-C₄-alkyl-C₆-C₁₀-aryl radical which can be substituted by halogen atoms.

The cations [A] are, for example, selected from trimethylphenyl ammonium, methyltrioctyl ammonium, tetrabutyl-phosphonium, 3-butyl-1-methyl-imidazolium, 3-ethyl-1-methyl-imidazolium, N-butyl pyridinium, N-ethyl pyridinium, diethyl pyrazolium, 1-ethyl-3-methylimidazolium, 1-butyl-3-methylimidazolium, 1-hexyl-3-methylimidazolium, 1-octyl-3-methylimidazolium, 1-decyl-3-methylimidazolium, 1-butyl-4-methylpyridinium, 1-butyl-3-methylpyridinium, 1-butyl-2-methylpyridinium, 1-butyl-pyridinium, butyl-methylimidazolium, nonyl-methyl-imidazolium, butyl-methylimidazolium, hexyl-methyl-imidazolium, octyl-methylimidazolium, 4-methyl-butyl-pyridinium, triethyl ammonium, triethylmethyl ammonium, butylmethylpyridinium, propyl ammonium, methyl-methyl-imldazolium, ethyl-methyl-imidazolium, butyl-methyl-imidazolium.

Ionic liquids and their production are known in the prior art. For the synthesis of ionic liquids using hexafluorophosphate, tetrafluoroborate, bis(trifluoromethylsulphonyl)amide, perfluoroalkyl sulphonate and perfluoroalkyl carboxylate ions, the corresponding halide salt [cation] ⁺X⁻ is first formed and isolated by reacting an amine NR₁N₂R₃, a phosphane PR¹R²R³, an imidazole derivative having the general formula R¹R²⁺N═CR³—R⁵—R³C═N⁺R¹R²or a pyridinium derivative having the general formula R¹R²N═CR³R⁴⁺ with an alkyl chloride, alkyl bromide or alkyl iodide (F. H. Hurley, T. P. Wier, Jr., J. Electrochem. Soc. 1951, 98, 207-212; J. S. Wilkes, J. A. Levisky, R. A. Wilson, C. L. Hussey, Inorg. Chem. 1982, 21, 1263-1264; A. A. K. Abdul-Sada, P. W. Ambler, P. K. G. Hodgson, K. R. Seddon, N. J. Steward, WO-A-95/21871) R. H. Dubois, M. J. Zaworotko, P. S. White, Inorg. Chem. 1989, 28, 2019-2020; J. F. Knifton, J. Mol. Catal. 1987, 43, 65-78; C. P. M. Lacroix, F. H, M. Dekker, A. G. Talma, J. W. F. Seetz, EP-A-0989134). Starting from the [A]⁺X⁻ halide salt which has been formed and isolated, two different paths are known for the synthesis of ionic liquids using hexafluorophosphate, tetrafluoroborate, bis(trifluoromethyl sulphonyl)-amide, perfluoroalkyl sulphonate and perfluoroalkyl carboxylate ions. On the one hand, the halide salt is converted by the addition of a metal salt MY (with precipitation or separation of the salt MX or the product [A]⁺[Y]⁻ from the respective solvent used) where [Y]⁻ stands for a hexafluorophosphate, tetrafluoroborate, bis(trifluoromethyl sulphonyl)amide, perfluoroalkyl sulphonate and perfluoroalkyl carboxylate ion and M⁺ stands for an alkali cation (J. S. Wilkes, M. J. Zaworotko, J. Chem. Soc. Chem. Commun. 1992, 965-967; Y. Chauvin, L. Muβmann, H. Olivier, Angew. Chem. 1995, 107, 2941-2943; P. A. Z. Suarez, J. E. L. Dullius, S. Einloft, R. F. de Souza, J. Dupont, Polyhedron, 1996, 15, 1217-1219; P. Bonhôte, A.-P. Dias, N. Papageorgiou, K. Kalyanasundaram, M. Grätzel, Inorg. Chem. 1996, 35, 1168-1178; C. M. Gordon, J. D. Holbrey, A. R. Kennedy, K. R. Seddon, J. Mater. Chem. 1998, 8, 2627-2638; P. A. Z. Suarez, S. Einloft, J. E. L. Dullius, R. F. de Souza, J. Dupont, J. Chim. Phys. 1998, 95, 1626-1639; A. J. Carmichael, C. Hardacre, J. D. Holbrey, M. Nieuwenhuyzen, K. R. Seddon, Anal. Chem. 1999, 71, 4572-4574; J. D. Holbrey, K. R. Seddon, J. Chem. Soc., Dalton Trans. 1999, 2133-2140). On the other hand, by the addition of a strong acid H⁺[Y]⁻ the halide ion is displaced with the release of H⁺X⁻ and exchanged for [Y]⁻, where [Y]⁻ here stands for a hexafluorophosphate, tetrafluoroborate, bis(trifluoromethylaulphonyl) amide, perfluoroalkyl sulphonate and perfluoroalkyl carboxylate ion (J. Fuller, R. T. Carlin, H. C. de Long, D. Haworth, J. Chem. Soc. Chem. Commun. 1994, 299-300). However, ionic liquids can especially advantageously be produced halide-free using the method described in EP 00118441.5.

In a development of the method according to the invention the ionic liquid is used as the only reaction medium, i.e., free from further solvents. The fraction of the ionic liquid in the reaction medium can however be between 0.1 and 99.9 percent by volume, preferably between 5 and 75 percent by volume, more preferably between 15 or 50 and 75 percent by volume relative to the total quantity of the reaction medium.

In addition to the ionic liquid, the reaction medium can also contain a further solvent. This can be selected from the group consisting of water, buffer solutions (pH 2 to 10, preferably 5 to 8) and organic solvents. Usable organic solvents are miscible with water or immiscible with water. AB examples of organic solvents mention may be made of methyl-tert-butyl ether, toluene, hexane, heptane, tert-butanol, glycols, polyalkylene glycols. In addition, however, fundamentally all conventional solvents known in the field of enzyme catalysis can be considered.

All enzymes in EC classes 1 to 6 can fundamentally be considered. The enzyme classification is recommended by the “Nomenclature Committee of the International Union of Biochemistry and Molecular Biology” (IUBMB). The enzyme is either homogeneously dissolved but can also be used as a suspension or as an immobilisate on an inert carrier.

It was found according to the invention that the presence of ionic liquids in the reaction medium during enzymatically catalysed reactions results in an improvement in the substrate solubility (biocompatibility), an improvement in the enzyme activity, an improvement in the selectivity, a reduction in product inhibition, suppression of aide reactions (parallel, consecutive reactions) and/or an increase in enzyme stability. The examples prove that enzymes from different classes can be used, wherein the use of ionic liquids offers significant advantages, such as, for example, an increase in the activity in the case of formate dehydrogenase, a significant increase in the yield in reactions with galactosidase, an increase in the enantioselectivity for lipases and an improvement in the educt solubility for hydrophobic educts.

According to the invention, the enzyme together with the total quantity of ionic liquid or a part thereof can be used repeatedly or in continuously operated reactors.

The enzymes can be selected from the class of oxidoreductases for regio- and stereoselective oxidation and reduction, from the class of glycosidases for the synthesis of oligosaccharides, from the class of lipases to obtain optically active products (among others, alcohols, amines, carboxylic acids) and from the class of lyases for synthesis, and hydrolases.

The method according to the invention can be carried out at temperatures between −10° C. and 130° C., preferably in a temperature range between 10° C. and 80° C., especially preferably in a temperature range between 20° C. and 40° C.

The method can be carried out in a single-phase fashion or in a multiphase reaction system.

Some effects arising from the use of ionic liquids as a reaction medium or as a constituent of reaction media for enzymatic reactions will be described below as examples. For example, alcohol dehydrogenases from various sources are used among others for the enzymatic reduction of ketones. The solubility of hydrophobic ketones can be improved by the addition of organic solvents; however, this generally results in a reduction in the enzyme activity and stability (W. Hummel, Biochem. Eng. Biotechnol. 1997, 58, 145; A. Liese, T. Zelinski, M. -R. Kula, H. Kierkels, M. Karutz, U. Kragl, C. Wandrey, J. Mol. Cat. B 1998, 4, 91). In a similar fashion, water-miscible ionic liquids can be added to the reaction medium to increase the educt solubility. For the formate dehydrogenase used for cofactor regeneration, an increase in the reaction rate compared with the purely aqueous system is observed in the same concentration range of the ionic liquid (see Example 1). No deactivation of the enzyme was observed even when the time of influence of the ionic liquid was fairly long. Thus, ionic liquids offer a valuable possibility for increasing the productivity of enzymatic reactions through an increase in the educt concentration. This is especially interesting for barely soluble to very barely soluble educts such as aromatic ketones or steroids.

For about 20 years glycosidases have not only been used for breaking bonds between saccharides but also for the synthesis of di- and oligosaccharides. Despite many attempts to use water-miscible solvents (leads to reduced enzyme stability), by means of the generally expensive activation of educts, yields no greater than 31% have been obtained even in the most recent studies (J. H. Yoon, J. S. Rhee, Carbohydr. Res. 2000, 327, 377; M. J. Hernaiz, D. H. G. Crout, J. Mol. Cat. B 2000, 10, 403). The main problem in these reactions is the immediately initiated secondary hydrolysis of the product, catalysed by the same enzyme. Surprisingly, this secondary hydrolysis is almost completely suppressed in the presence of ionic liquids with otherwise the same enzyme activity. For the example of β-galactosidase-catalysed synthesis of N-acetyl lactosamine, an important building block for pharmacologically relevant oligosaccharide, it was shown that the presence of ionic liquids increases the yield above 55% when using lactose as an inexpensive donor. A maximum of 30% is achieved without the addition of ionic liquids; however, the product concentration drops rapidly to values of <10% as a result of the secondary hydrolysis. Since the secondary hydrolysis does not take place in the presence of ionic liquids, a simplified reaction process is obtained since it is not necessary to follow the reaction and interrupt it at the maximum product yield. The galactosidase is very stable in the presence of ionic liquids and can be repeatedly used after separation by means of ultrafiltration without any change in the attainable yield and the product formation rate.

Ionic liquids also offer advantages in reverse hydrolysis for the synthesis of di- and oligosaccharides. In this case, high educt concentrations together with additives, usually organic solvents, are used to reduce the water activity. Here ionic liquids especially offer the advantage of a very good dissolving capacity for carbohydrates. In the enzymatic synthesis of lactose, it was possible to increase the yield by a factor of 2 and reduce the reaction time by a factor of 5 compared with the literature (K. Ajisaka, H. Fujimoto, H. Nishida, Carbohydr. Res. 180, 35-42 (1988)).

The use of lipases in the presence of organic solvents in single- or two-phase reactions is prior art (G. Carrea, S. Riva, Angew. Chem. 2000, 112, 2312, U. T. Bornscheuer, R. J. Kazlauskas, Hydrolases in Organic Synthesis—Regio- and stereoselective Biotransformations, Wiley-VCH, Weinheim, 1999, A. Liese, K. Seelbach, C. Wandrey, Industrial Biotransformations, Wiley-VCH, Weinheim, 2000; M. C. Parker, S. A. Brown, L. Robertson, N. J. Turner, Chem. Commun. 1998, 2247). However, the enzyme has so far conventionally been separated by filtration and the reaction solution conventionally separated by distillation and the solvent fed back. The use of ionic liquids permits direct distillative separation of the reactands from the reaction mixture even in the presence of the enzyme so that a simplified procedure results. If the reactands possess suitable volatility, this procedure is not limited to lipases. In an investigation of various lipases for racemate splitting in the presence of ionic liquids it was surprisingly established in several cases that the conversion rate and the enantioselectivity are in some cases significantly improved, in individual cases a factor of 5 better. The reaction in tert-butylmethyl ether which is also used as a solvent for lipase-catalysed reactions in industrial processes, is used for comparison.

The results show that ionic liquids as a reaction medium for enzymatic conversions have numerous advantages compared with the conditions established as the prior art and can be used as biocompatible solvents to specifically influence conversions.

The invention is described in detail by the following examples without however being restricted to these.

EXAMPLES

The following abbreviations were used for the description of components used in the examples:



tert-butylmethylether	tBME,	MTBE
Butyl-methyl-imidazolium PF₆ ⁻	BMIm⁺	PF₆ ⁻
Nonyl-methyl-imidazolium PF₆ ⁻	NMIm⁺	PF₆ ⁻
Butyl-methyl-imidazolium BF₄ ⁻	BMIm⁺	BF₄ ⁻
Hexyl-methyl-imidazolium BF₄ ⁻	HMIm⁺	BF₄ ⁻
Octyl-methyl-imidazolium BF₄ ⁻	OMIm⁺	BF₄ ⁻
4-methyl-butyl-pyridinium BF₄ ⁻	4-MBPy⁺	BF₄ ⁻
Triethylammonium-methyl sulphate	Et₃NH⁺	MeSO₄ ⁻
Triethylmethylammonium-methyl	Et₃NMe⁺	MeSO₄ ⁻
sulphate
Butylmethyl-pyridinium BF₄ ⁻	BMPy⁺	BF₄ ⁻
Propylammonium-nitrate	PrNH₃ ⁺	NO₃ ⁻
Methyl-methyl-imidazolium-methyl	MeSO₄ ⁻
sulphate	MMIm⁺
Ethyl-methyl-imidazolium-benzoate	EMIm⁺	PhCO₂ ⁻
Butyl-methyl-imidazolium-trifluoro	BMIm⁺	CF₃SO₃ ⁻
methane sulphonate
(=triflate)
Butyl-methyl-imidazolium-bis-	BMIm⁺	(CF₃SO₂)₂N⁻
trifluoromethyl		sulphonyl)-imidate

1. Formate Dehydrogenase from [0057] Candida boidinii (FDH)
The FDH-catalysed oxidation of formic acid to carbon dioxide by the reduction of nicotinamide adenine dinucleotide (NAD[0058] ⁺ to NADH+H⁺) is used as the test reaction to determine the enzyme activity. In the enzyme assay the increase in NADH with time at 25° C. is detected photometrically at a wavelength of 340 nm.
Composition of the enzyme assay: 1 ml buffer solution (50 mM triethanolamine hydrochloride, 1 mM dithiothreitol, hydrochloric acid) pH 7 is mixed with 0.1 ml of aqueous sodium formate solution (2.4 M) and 0.1 ml of enzyme solution (0.7 mg/ml, 8.4 U). The enzyme solution already contains the cofactor NAD (6 mM). [0059]
In order to test the influence of water-soluble ionic liquids on the enzyme activity, the volume of the buffer solution in the assay is gradually reduced by 25 vol % and replaced by the ionic liquid. [0060]

TABLE 1

NAD reduction by formate dehydrogenase from

Candida boidinii; enzyme activity compared

with the standard reaction in the buffer solution

Ionic liquid 25 vol % 50 vol % 75 vol%

MMIm⁺ MeSO₄ ⁻ ± + +
2. β-galactosidase from [0061] Bacillus circulans for Synthesis of N-acetyl Lactosamine
The influence of ionic liquids on the progress of β-Gal-catalysed tranagalactosylation starting from lactose and N-acetyl glucosamine is studied. For this purpose concentration-time profiles of this synthesis in the presence and absence of ionic liquids are recorded and compared. [0062]
In each series of tests 10 reactions were started in parallel in 1 ml GC glasses. At 10 minute intervals the reactions are stopped by boiling at 100° C., the reaction solution is filtered (Minisart RC 4 Sartorius injection filter) and the concentration of the reaction components at this time is determined chromatographically (Aminex HPX-87H cation exchange column from BioRad with a corresponding pre-column, 0.006 M sulphuric acid as eluent with a flux of 0.8 ml/min and a column temperature of 65° C. Detection is accomplished using UV at 208 nm and using the refractive index). [0063]
Composition of the reaction mixture: 0.05 ml buffer solution (65 mM KH[0064] ₂PO₄, 195 mM K₂HPO₄) pH 7,3 is mixed with 0.5 ml N-acetyl glucosamine solution (GlcNAc 600 mM or 1.2 M in buffer solution), 0.25 ml lactose solution (250 mM in buffer solution) and 0.2 ml enzyme solution (10 mg/ml in buffer solution).
By exchanging buffer solution for ionic liquid in the substrate solutions, the fraction of ionic liquid in the reaction medium is gradually increased. The following substrate solutions are thus obtained: [0065]
a) 0.50 ml N-acetyl glucosamine solution (600 mM in 1:4 MMIm[0066] ⁺ MeSO₄ ⁻: buffer solution)
b) 0.50 ml N-acetyl glucosamine solution (600 mM in 1:4 MMIm[0067] ⁺ MeSO₄ ⁻: buffer solution) 0.25 ml lactose solution (250 mM in 1:4 MMIm⁺ MeSO₄ ⁻: buffer solution)
c) 0.50 ml N-acetyl glucosamine solution (1.2 M in 1:2 MMIm[0068] ⁺ MeSO₄ ⁻: buffer solution) 0.25 ml lactose solution (250 mM in 1:2 MMIm⁺ MeSO₄ ⁻: buffer solution)

d) 0.50 ml N-acetyl glucosamine solution (600 mM in 1;4 BMIm ⁺ H₂PO₄ ⁻/Cl⁻: buffer solution)

TABLE 2


Synthesis of N-acetyl lactosamine by β-
galactosidase from Bacillus circulans with and
without ionic liquid

		Lactose/	Yield	Yield
Reaction	Fraction	GlcNAc	[%]	[%] after
medium	[vol %]	ratio	after 60 min	100 min

Phosphate		1:2.4	5	3
buffer
MMIm⁺ MeSO₄ ⁻	a) 12.5	1:2.4	40	40
	b) 18.75	1:2.4	44	43
	c) 25	1:4.8	49	55
				(90 min)
BMIm⁺ H₂PO₄ ⁻/	d) 12.5	1:2.4	39	30
Cl⁻

3. Enantioselective Acylation of R,S-1-Phenylethanol by Catalysis with Lipase from [0070] Candida antarctica (Type B) in Ionic Liquids
4.4 ml of an ionic liquid in accordance with Table 3 or tert-butylmethyl ether are mixed with 122 μl of vinyl acetate and 54 μl of 1-phenyl ethanol so that a substrate solution with approximately 0.1 mol/l of 1-phenyl ethanol and 0.3 mol/l of vinyl acetate is obtained. Each 1 mg of lyophilised lipase (>120 U/mg) is mixed with 0.4 ml of substrate solution, thoroughly mixed and incubated at 24° C. for 3-4 days, shaking slightly. [0071]
For further processing 100 μl of the reaction formulation is mixed with 1 ml of n-hexane/isopropanol (97.5/2.5 v/v) and mixed thoroughly. This hexane/isopropanol extract is used to determine the concentrations and enantiomer ratios of 1-phenyl ethanol and 1-phenylethyl acetate using HPLC. The conversion and the enantiomer excess were calculated from these concentrations (see Table 3). [0072]

HPLC Conditions:



	Column:	Guard column Nucleosil C-18 5 μm; 10 mm,
		4.6 mm ID;
		Preparatory column Chiracel OJ; 50 mm,
		4.6 mm ID;
		Separating column Chiracel OJ; 250 mm,
		4.6 mm ID
	Eluent:	isocratic;
		96.5% (v/v) n-hexane,
		3.0% (v/v) isopropanol,
		0.5% (v/v) ethanol
	Flux rate:	1 ml/mm
	Temperature:	38° C.
	Detection:	UV detector (205 nm)

TABLE 3


Enantioselective acylation of 1-phenyl ethanol, catalysed by
lipase from Candida antarctica (Type B): conversion and enantiomer
excess in ionic liquids compared to the standard reaction in
tert-butylmethyl ether; (+) good to better, (±) the same,
(−) poor to none.

Ionic liquid/solvent	Conversion	Enantiomer excess

NMIm⁺ PF₆ ⁻	±	+
BMIm⁺ BF₄ ⁻	+	+
HMIm⁺ BF₄ ⁻	±	+
OMIm⁺ BF₄ ⁻	+	+
4-MBP⁺ BF₄ ⁻	+	+
BMIm⁺ CF₃SO₃ ⁻	+	+
BMIm⁺ (CF₃SO₂)₂N⁻	+	+

4. Enantioselective Acylation of R,S-1-Phenyl Ethanol by Catalysis with Lipase from [0075] Candida antarctica (Type A) in Ionic Liquids

Each 5 mg of lyophilised lipase (>30 U/mg) is mixed with 0.4 ml of a substrate solution as in Example 3. The further procedure corresponds to that described in Example 3.

TABLE 4


Enantioselective acylation of 1-phenyl
ethanol, catalysed by lipase from Candida
antarctica (Type A): conversion and
enantiomer excess in ionic liquids compared
to the standard reaction in tert-butylmethyl ether;

Ionic liquid/solvent	Conversion	Enantiomer excess

BMIm⁺ PF₆ ⁻	+	+
NMIm⁺ PF₆ ⁻	+	+
BMIm⁺ BF₄ ⁻	±	+
HMIm⁺ BF₄ ⁻	+	+
OMIm⁺ BF₄ ⁻	+	±
BMIm⁺ CF₃SO₃ ⁻	+	+

5. Enantioselective Acylation of R,S-1-Phenyl Ethanol by Catalysis of Lipase from Pseudomonas _sp. in Ionic Liquids [0077]

Each 3 mg of lyophilised lipase (400 U/mg) is mixed with 0.4 ml of a substrate solution as in Example 3. The further procedure corresponds to that described in Example 3.

TABLE 5


Enantioselective acylation of 1-phenyl
ethanol, catalysed by lipase from Pseudomonas
sp.: conversion and enantiomer excess in
ionic 1iquids compared to the standard
reaction in tert-butylmethyl ether;

Ionic liquid/solvent	Conversion	Enantiomer excess

MIm⁺ PF₆ ⁻	±	+
4-MBP⁺ BF₄ ⁻	±	+
BMIm⁺ CF₃SO₃ ⁻	+	+
BMIm⁺ (CF₃SO₂)₂N⁻	+	+

6. Enantioselective Acylation of R,S-1-Phenyl Ethanol by Catalysis of Lipase from Alcaligines sp. in Ionic Solvents [0079]

Each 5 mg of lyophilised lipase (>20 U/mg) is mixed with 0.4 ml of a substrate solution as in Example 3. The further procedure corresponds to that described in Example 3.

TABLE 6


Enantioselective acylation of 1-phenyl
ethanol, catalysed by lipase from Alcaligines
sp.: conversion and enantiomer excess in
ionic liquids compared to the standard
reaction in tert-butylmethyl ether,

Ionic liquid/solvent	Conversion	Enantiomer excess

BMIm⁺ PF₆ ⁻	±	+
BMIm⁺ BF₄ ⁻	±	+
HMIm⁺ BF₄ ⁻	±	+
OMIm⁺ BF₄ ⁻	±	+
4-MBP⁺ BF₄ ⁻	±	+
BMIm⁺ CF₃SO₃ ⁻	±	+

7. Recycling of the Lipase from [0081] Candida antarctica (Type B) in Ionic Liquids by Distillation
600 mg of lyophilised lipase (approx. 10 U/mg) is mixed with 4 ml of ionic liquid (BMIm[0082] ⁺ (CF₃SO₂)₂N⁻), 1.2 ml of vinyl acetate and 0.7 ml 1-phenyl ethanol and thoroughly mixed. The reaction mixture is incubated for 40 min at 40° C.
The non-converted educts and the reaction product 1-phenyl acetate is then distilled off (85° C., 0.06 mbar). [0083]
The enzyme/ionic liquid mixture is cooled, re-mixed with 1.2 ml of vinyl acetate and 0.7 ml of 1-phenyl ethanol and again incubated for 40 min at 40° C. [0084]
The reaction sequence involving incubation and distilling off can be repeated many times without the lipase activity diminishing. [0085]
8. Synthesis of Lactose by Reverse Hydrolysis with β-galactosidase from [0086] Bacillus circulans
100 mmol/l of glucose, 20 mmol/l of galactose and 2 mg/ml of galactosidase are incubated at 35° C. in a mixture of water and MMIm MeSO[0087] ₄for 24 h. The reaction is stopped by boiling for 10 minutes at 100° C., the reaction solution is filtered (Minisart RC 4 Sartorius injection filter) and the concentration of the reaction components at this time is determined chromatogaphically (Aminex HPX-87H cation exchange column from BioRad with corresponding preparatory column, 0.006 M sulphuric acid as eluent with a flux of 0.8 ml/min and a column temperature of 65° C. Detection is accomplished using UV at 208 nm and using the refractive index).
The fraction of ionic liquid is increased from 0 to 100 per cent by volume. As a result of water traces in the ionic liquid and the educts, a water content of 0.6% is obtained for 100% ionic liquid. After 24 hours, the conversion no longer increases. The following lactose yields are obtained depending on the quantity of ionic liquid: [0088]

Praction of ionic liquid in % Yield [%]

0 0

10 0

20 0

30 4

40 12

50 15

60 15

70 16

80 16

90 16

100 17

Claims

1. A method for the conversion of substances in the presence of enzymes as a catalyst in a reaction medium comprising at least one ionic liquid, wherein the enzyme is selected from the group of oxidoreductases, lipases, galactosidases, glycosidases, lyases and enzymes in EC class 6.

2. The method according to claim 1, characterized in that the ionic liquid has the general formula

[Al_n ⁺[Y]ⁿ⁻,

where

n=1 or 2 and

the anion [Y]ⁿ⁻ 0 is selected from the group comprising tetrafluoroborate ([BF₄]), tetrachloroborate ([BCl₄]⁻), hexafluorophosphate ([PF₆]⁻), hexafluoroantimonate ([SbF₆]⁻), hexafluoroarsenate ([AsF₆]⁻), tetrachloroaluminate ([AlCl₄]⁻), trichlorozincate (ZnCl₃]⁻), dichlorocuprate, sulphate ([SO₄]²⁻), carbonate ([CO₃]²⁻), fluorosulphonate, [R′—COO]⁻, [R′—SO₃]⁻ or [(R′—SO₂)₂N]⁻, and R′ is a linear or branched aliphatic or alicyclic alkyl containing 1 to 12 carbon atoms or a C₅-C₁₈-aryl, C₅-C₁₈-aryl-C₁-C₆-alkyl or C₁-C₆-alkyl-C₅-C₁₈-aryl radical that can be substituted by halogen atoms,

the cation [A]⁺ is selected from

quaternary ammonium cations having the general formula

[NR¹R²R³R]⁺,

phosphonium cations having the general formula

[PR¹R²R³R]⁺,

imidazolium cations having the general formula

where the imidizole nucleus can be substituted with at least one group selected from C₁-C₆-alkyl, C₁-C₆-alkoxy, C₁-C₆-aminoalkyl, C₅-C₁₂-aryl or C₅-C₁₂-aryl-C₁-C₆-alkyl groups,

pyridinium cations having the general formula

where the pyridine nucleus can be substituted with at least one group selected from C₁-C₆-alkyl, C₁-C₆-alkoxy, C₁-C₆-aminoalkyl, C₅-C₁₂-aryl or C₅-C₁₂-aryl-C₁-C₆-alkyl groups,

pyrazolium cations having the general formula

where the pyrazole nucleus can be substituted with at least one group selected from C₁-C₆-alkyl, C₁-C₆-alkoxy, C₁-C₆-aminoalkyl, C₅-C₁₂-aryl or C₅-C₁₂-aryl-C₁-C₆-alkyl groups,

and triazolium cations having the general formula

where the triazole nucleus can be substituted with at least one group selected from C₁-C₆-alkyl, C₁-C₆-alkoxy, C₁-C₆-aminoalkyl, C₅-C₁₂-aryl or C₅-C₁₂-aryl-C₁-C₆-alkyl groups,

and the radicals R¹, R², R³are selected independently of one another from the group consisting of

hydrogen;

heteroaryl, heteroaryl-C₁-C₆-alkyl groups with 3 to 8 carbon atoms in the heteroaryl radical and at least one heteroatom selected from N, O and S which can be substituted with at least one group selected from C₁-C₆-alkyl groups and/or halogen atoms;

aryl-, aryl-C₁-C₆-alkyl groups with 5 to 12 carbon atoms in the aryl radical which if necessary can be substituted with at least one C₁-C₆-alkyl group and/or one halogen atom.

3. The method according to any one of the preceding claims, characterised in that the fraction of the ionic liquid in the reaction medium is 0.1 to 99.9 percent by volume.

4. The method according to any one of the preceding claims, characterised in that in addition to the ionic liquid, the reaction medium also contains a further solvent.

5. The method according to any one of the preceding claims, characterized in that the further solvent is water or an organic solvent.

6. The method according to any one of the preceding claims, characterised in that the reaction is carried out at temperatures of −10° C. to 130° C.

7. The method according to any one of the preceding claims, characterised in that the reaction is carried out in a single-phase fashion or in a multiphase reaction system.

8. A composition comprising an enzyme selected from the groups of oxidoreductases, lipases, galactosidases, glycosidases, lyases and enzymes in EC class 6 and at least one ionic liquid.

9. The composition according to claim a, characterised in that the ionic liquid is defined as in claim 2.

10. The composition according to claim 8 or claim 9, characterised in that it additionally contains a substrate.

11. The composition according to any one of claims 8 to 10, characterised in that it contains the ionic liquid as a reaction medium or as a constituent of the reaction medium.

12. Use of ionic liquids as a reaction medium or as a constituent of the reaction medium for enzymatically catalysed reactions, wherein the enzyme is selected from the group of oxidoreductaaes, lipases, galactosidases, glycosidases, lyases and enzymes in EC class 6.

13. The use according to claim 12 as a reaction medium or as a constituent of the reaction medium in reverse hydrolysis for the synthesis of di- and oligosaccharides.