UA77607C2 - Stimulant of growth and development of cereals and a method to stimulate growth and development of maize and wheat - Google Patents

Abstract

A growth and development stimulator of cereals and a method to stimulate the growth and development of maize and wheat is disclosed. A method provides for presowing treatment of the maize and wheat seed by means of spraying thereof by aqueous solution of polyhexamethylenguanidine hydrochloride of a molecular weight of 5-9 thousand of standard units and with concentration of 0.01-0.05% at a rate of 35-40 l per 1 ton of the seed, including growing thereof on the herbicidal background.

Description

control of the growth and development processes of corn and wheat by means of pre-sowing seed treatment by spraying. Spraying is carried out with 0.01-

with a 0.0% solution of polyhexamethyleneguanidine

MN. NOA and hydiochloride

The use of this invention for the application of -(snu5-MN-s--Mmn-- allows to increase the efficiency of agricultural production, the yield of grain crops, namely, corn and wheat, which is confirmed by the following examples 1-4 and with the molecular weight of 5-9 thousand units at a dose of Table 1-7. 35-40 l per 1 ton of seeds, including when growing

It is known that succinic acid is widely used in the protection of grain crops against a herbicide background. in crop production to increase the yield of grain- The essence of claimed inventions on new crops

R.R. The biological nature of increasing the yield is 4. Tables 1-7 show the average data of 20-100 nos with the help of succinic acids. - M.: Moscow State University, definitions, repeatability of experiments - 4-fold. In 1970, p. 61), when pre-sowing seed treatment in model experiments of corn and spring wheat sprouts is carried out with 0.4-0.6 Uonim solution, wheat was grown in rolls of filterable succinic acid, which stimulates the growth of plants and paper in an aqueous environment or herbicide solution increases the grain yield | (Kalinin F.L. Biologichedu at - 25"С in a thermostat (up to 4 days), in Podalsk, active substances in crop production. Kyiv: Shomu - at room temperature and lighting

Scientific opinion, 1984.-318 p.|. daylight lamps; germination energy

However, the disadvantages of this method should be determined on the fifth day, similarity - on the seventh; to calculate the high cost and large costs prevented the selection of sprouts and measurements of the lengths of the roots of ratu due to its rather high concentrations, (1) and sprouts (I). as well as slight stimulation of germination Statistical processing of the obtained seeds was carried out. experimental data according to standard methods

Also known is the method of pre-sowing treatment of the dam |Rokytskyi P.fF. Biological statistics. cereal seeds to stimulate germination, Minsk, Vysheysha school, 1973. - 318 p. | Deviation from the average for each statistical series of non-wheat by soaking the seeds during sowing - 1870 exceeds 5 95 from the average values. in an aqueous solution of shydroperite (Association of the USSR Example 1. Pre-sowing treatment of seeds of ku-

Me1387890, class АО1С1/00, bull. Mo 14 dated 15.04.88), kuruzy of two hybrids - ZPTK 196 (early ripening) and which is taken as a prototype for the process that stated - Cadre 267 MV (mid-early); as well as winter rain. Odeska 161 wheat and spring wheat - Kolektivna Z

Among the disadvantages of the known method should be attributed to aqueous solutions of polyhexamethyleneguanidine - the laboriousness of the process of soaking seeds in dihydrochloride (0.001-0.195) led to growth, as well as the fact that the field germination of seeds does not increase the energy of germination and germination of seeds in wheat after treatment with this by the method of increasing experiments on 7-2195 in comparison with las only by 695, the height of plants - by 595 from the control (k.), including on the herbicidal background of troll, the yield - by 995 in comparison with non- (0 ,00596-th harness solution), reduction by processed seeds, and susceptibility of plants to chewing. seeds with phytopathogenic microflora in diseases decreased by 20965. in most cases to zero (Tables 1, 2), which re-

Intensive use in the rural economy shows the corresponding indicators of the prototype. application of herbicides and their mixtures leads to Example 2. The effect of pre-sowing some slowing of growth rates and the development of seed treatment of two hybrids of corn and bee-culture plants, which can negatively affect the growth of not only development of agricultural bulbs and the development of sprouts in the early stages of ontogenesis, especially under conditions of water deficit and on clean and herbicide background in model climate warming, but also on the quality of agricultural experiments. Stimulation of the growth of gift products has been revealed. indicators, including on a herbicide background, for

The basis of the invention is the task of expanding the assortment (tables 3, 4) for experimental variants of pre-sowing biologically active substances - growth stimulators by treating seeds with solutions for the claimed process for corn - for 12-5295, for wheat - for 10-3895 Polyhexamethyst - grain crops that reduce the negative lenguanidine hydrochloride. Stimulation of the effect of herbicide treatment of crops on the cultural morphometric indicators of corn and wheat plants, reduction was revealed. labor-intensive processing after pre-sowing seed treatment with seed solutions, increase in its germination (germination), and PGMG significantly exceeds the indicators of the prototype. also, increasing the yield of grain crops. Optimum for seed treatment research

This task is solved in such a way that the concentration of PGMG in cereal crops is in the range of 0.01-0.05965 as a growth and development stimulator. of new cultures, PGMG was applied to stimulate

Table 1

The effect of pre-sowing seed treatment with PGMG (polyguanidine) solutions on the energy of germination, germination, susceptibility of maize seeds of hybrids Kadr 267 MV and ZPTK 196 on a clean and herbicide background

Name type of processing sprouting, 9o so sprouting, 905 so chewiness, 905

Control, without 0.1 95 percent polyguanidine 92 95 (0) 92 95 (0) 0.0595 -7- 99 100 (0) 97 99 (0) 0.049 -7- 95 96 (0) 92 94 (0) 0.039 -7- 95 98 (0) 93 94 (0) 0.029 -7- 97 98 (0) 95 99 (0) 0.019 -7- 98 99 (0) 95 97 (0) 0.00195 -7 - 98 100 (9) 92 92 15 0.00595 harness

Control, without 75 7v 45 vv5 42 0.1 95 polyguanidine 90 90 (0) 92 94 (9) 0.0595 -7- 95 99 (0) 96 98 (0) 0.049 -7- 92 92 (0) 90 92 (0) 0.039 -7- 92 94 (0) 92 92 (9) 0.029 -7- 95 94 (0) 95 98 (0) 0.019 -7- 92 94 (0) 95 99 (0) ) 0.00195 -7- 90 90 (9) 90 92 12

The sampling error does not exceed 595 of the mean values

Table 2

The influence of pre-sowing treatment of Odeska 161 (winter) wheat seeds and

Collective With (spring) solutions of polyguanidine on the energy of germination, germination, impressionability of seeds

Odesa Wheat 161 Clear background, water

Control, without treatment 65 7 0.059 r-n polyguanidine 68 69 4 0.029 -7- 67 69 (0) 0.0195 -7- 66 69 (9) chicken 1 le | 8 | with

Control, without treatment b2 63 0.059 r-n polyguanidine 66 70 (9) 0.029 -7- 70 71 (9) 0.019 -7- 70 70 (0)

Wheat Collective Z

Clean background, water

Control, without treatment 84 85 10 00596 r-npolyanidin. 81 81 5

Control, without processing 82 85 12 (00596 r-npolyanidin.d 77771 І777777771717961111111 11117196 | that

The sampling error does not exceed 595 of the mean values

Table C

The effect of pre-sowing seed treatment with polyguanidine solutions on the morphometric parameters of corn sprouts of hybrids Kadr 267 MV and ZPTK 196 on a clean and herbicide background

Name of the sample, tef in) enrya It is from k. from k. from k. from k. Kk. from k. 267 MB

Control, untreated 127| 100| 67 1001 131 | 100 72 100 145| 7100| 88 100 0.195 polyguanidine 131) 1031 71 1051 1481 113 80 110) 164| 1131 92 105 0.0595 -7- 160| 1261 1001 1501 1611 123 99 138| 1751 1211 1261 143 0.049 -7- 163| 128| 77 1151 1741 133 84 116 | 190 | 131 92 105 0.039 -7- 150) 118| 70 1051 1521 116 81 113) 168 116) 92 105 0.029 -7- 156| 122| 84 1251 1561 119 85 118| 194) 134) 1291 147 0.019 -7- 142| 112| 89 1331 1511 115 91 127 | 178) 1231 1261 143 0.00195 -7- 145| 114| 80 1201 147 | 112 81 112| 172| 119) 97 110 0.0059oharnes nu 100 48 1001 102 100 57 100| 106|100| 58 100 0.195 polyguanidine 111 1131 57 1181 1191 117 65 114| 123| 116| 73 127 0.059 -7- 119 | 121 55 1141 1271 125 67 118 133) 125| 74 128 0.039 -7- 118) 120| 58 1211 1161 114 66 1151 122| 115) 67 115 0.029 -7- 1131 1131 52 1091 1241 122 bo 1051 133) 125| 65 112 0.019 -7- 119 | 121 55 1141 1151 113 67 118) 126| 119) 67 115 0.00195 -7- 120| 122| 67 139 | 112| 110 70 122| 1191 112) 77 132 196

Clean background, water Con- 130| 100) 94 100| 134 | 100 95 100| 136) 100) 1001 100 0.029 -7- 157 | 121) 104 111 168) 1251) 1171 1231 1761 1291 1521 152 0.0195 -7- 138| 106) 106| 113) 144) 108| 111 117| 144) 106) 132| 132 0.00595 harness Sh 92 | l100| 62 | 1001 94 | 100) 64 | 100 1001 71 | 100 0.029 -7- 99 108| 73 118 103 110 78 122| 106| 108 83 117 0.0195 -7- 97 | 105| 76 123| 96 102 80 125| 102 104| 93 131

Error, the sample does not exceed 595 of the mean values

Table 4

The effect of pre-sowing seed treatment with polyguanidine solutions on the morphometric parameters of Odeska 161 and Kolectivna Z wheat sprouts on a clean and herbicide background in model experiments

Name of the sample, type of processing mm | Uevedk.| mm | Fvidk.| mm | Uevidk.| mm | 95%

Clean background, water 71 100 5О 100 100 | 85 100 0.029 -7- 73 103 55 110 88 110 94 110 0.0195 -7- 72 101 50 100 85 106 89 105

Herbicidal background, dialen C - 50 pip control of beooorobki 2 | 00 | 8 | about | that) oo | ve) Comrade

Continuation of table 4

Name of the sample, type of processing mm | Witness mm | Fvidk| mm | Uevidk.| mm | 9 records 0.059 polyguanidine 78 115 58 121 76 109 85 104 0.029 -7- 83 122 66 138 86 123 96 117 0.0195 -7- 77 113 65 135 78 111 84 102

Clean background, water 34 100 19.) 100 | 40 | 700 | 39 | 100

Herbicidal background, dialen C - 50

The sampling error does not exceed 595 of the mean values

Example 3. The effect of pre-sowing on the growth of the content of water-soluble proteins, dust treatment of seeds of two hybrids of corn and wheat on the activity of proteolytic enzymes (pH with aqueous solutions of PGMG (0.01-0.0595) by 7.7) and trypsin inhibitor was studied. as well as growth growth indicators of sprouts in field conditions of the content of free amino acids in roots and sprouts (Dnipropetrovsk, Cherkasy region; 2004); of corn and wheat in experimental variants in the general susceptibility of corn plants to phytopatho- of most samples after pre-sowing treatment with genetic infection (soot), the full height of plants and seeds with PGMG solutions (Table 7). This indicates the yield (tables 5, 6). Stimulation of the intensification of hydrolytic processes, as well as the growth of corn sprouts (on 10-4595) and the biosynthesis of proteins during seed germination in corn (on 13-4795), including on the herbicidal sah of growth and development of sprouts, were found. which of course is in the background. A decrease in the susceptibility to downy mildew was observed in cereals on a herbicide background. Identified corn plants with soot by 3-5 times (Table 5), changes in biochemical indicators of protein and amino acids - an increase in the total height of plants by 8 - 16905 and lot exchanges in the sprouts of cereal crops - grain yield - by 8 - 4995 in both hybrids of corn and wheat after the pre-sowing treatment of corn, which significantly exceeds the corresponding indicators - seeds with PGMG solutions indicate the strengthening of the prototype. adaptive processes in plant organisms at

Example 4. The effect of pre-sowing adverse environmental conditions was studied, including the treatment of seeds of two hybrids of corn and winter wheat on a herbicide background, which confirms the positive effect of wheat with aqueous solutions of PGMG (0.01-0.0595) on the effect of the proposed process on growth and development biochemical indicators of sprouts grown in field crops, especially under intensive conditions (Dnipropetrovsk, Cherkasy region; application of herbicides in modern field conditions, 2004), including on a herbicide background. Manifestations of agricultural production.

Table 5

Effect of pre-sowing treatment of seeds of maize hybrids Kadr 267 MV and ZPTK 196 with polyguanidine solutions on morphometric indicators of sprouts (phases 1-5 leaves), susceptibility and full height of plants, yield in field conditions (2004)

Cheers to the sample, the type of roots of the sprouts of the roots of the sprouts | r zvn eri i Teo eTayo z 1 ee yaeTao from k. from k. from k. from k. from k. from k.

Cadre 267 MV

Clean background

Control, without 70| 100 | 1351 100| what8| h00| 241 | 100| 25 | 176 | 100 100 processing o - from outside polygu- | 901 129| 1 FIGHTS 411 | 130| 120| zó51| 127| 1.0 | 1851 105| 104 | 106 0.029 -7- 79 1131 1651 122| 111/| 103) 322| 134| 08| 184| 105| 106 108 0.0195 -7- 76| 1091 171| 127| 122| 113| 323| 134| 03| 190| 108 99 | 101

Continuation of table 5

Hooray

The name of the chewing gum. Full pronunciation.

Herbicidal background, harness - 2 l/ha

Control, without BI| 100 | 106 | 100 | 105| 100 | 226 | 100 167 | 100| 84 | 100 processing sun BO| 116 | 133| 1251 h410| 4151 242| 107| 2.3 | 169| 101 | 125 | 149 0.029 -7- 751| 147) 1331 1251 1221 116| 3121 1381 1.81 194) 116 104 | 124 0.0195 -7- 73| 1431 146| 138| 118) 113| 262| 116| 0.3 | 169| 101| 107| 127

Corn ZPTK 196

Clean background

Control, untreated 1321 1001 2481 1001 31 | 276| 1001 92 | 100 0.05905 percent polyguanidine 150| 1141 261| 105| 1.2 | 2911 1051 1051 114 0.029 -7- 1531 116) 2581 1041 1.01 2991 1081 100| 109 0.0195 -7- 169| 1291 2651 107| 0.31 302) 1101 104 | 113

Herbicidal background, merlin - 130 g/ha

Control, no treatment 104 100 2461 1001 11.51 266| 1001 87 | 100 0.056 district polygu- 153| 146 | 279| 113| 32 | 292| 4110 | 100 | 115 of anidine 0.029 -7- 156| 149) 264| 107 3.51 287| 1081 89 | 102 0.0195 -7- 148| 142) 287) 116) 0.3 | 296 | 111 91 105

The sampling error does not exceed 595 of the mean values

Table 6

The influence of pre-sowing treatment of Perlyna Lisostepu wheat seeds with polyguanidine solutions on the morphometric indicators of sprouts (phase 4-5 leaves), the length of roots, the above-ground part, and the weight-raw weight (per plant) of wheat (emergence phase) in field conditions (Cherkasy Region, 2004)

The phase of exit into the tube - і і і І of above-ground plants

Name of the sample, 1 roots | 1 sprouts of 1 root part of the root | overhead system part oo oo Fo from Fo from g Fo from g oo from k. from k. Kk. Kk Kk from k.

Pearl winter wheat

Forest-steppe Herbicidal background, dialen C - 2 l/ha

Control, without processing b2 | 100| 191| 1001 133| 100 | 560| 1001 0.431 100 | 1.8 100 -0.02965 r-n of polyguanidine| 89 | 145 | 259| 136| 162| i22| 570| 102| 051) 119 | 20| 110

The sampling error does not exceed 595 of the mean values

Table 7

The influence of pre-sowing treatment of corn and wheat seeds with polyguanidine solutions on the biochemical parameters of sprouts (4-5 leaf phase) grown in the field (2004) - Proteolytic ac- Specific activity Free amino acid weights - - protein min. mg/g protein weight 1.3 12.8 58.8 10.8. 0.05965 polyguanidine 1.5 171 64.5 13.7 0.0196 -- 1.5 33.3 188.4 16.7

Herbicide background, harness - 2 l/ha Con- 0.05965 р-n of polyguanidine 1.3 10.3 127 16.4 00195 -- 21 27.9 156 20.5 poshi 210119»

Pure background 15115 0.029 -- 8.0 25.0 5.8 0.0196 -- 7.6 39.5 16.0 prolbeoobrobii 00702018 trol, without processing 0.0595 polyguanidine 12.6 22.0 8, 0

Е.

Pure background 0.029 -- 1.80 26.5 360.0 34.4 00195 -- 1.40 21.4 62.4 11.0

Herbicidal background, merlin - 130 g/ha 0.05965 r-n polyguanidine 1.40 26.0 273.0 20.4 00196 -- 1.34 46.0 244.5 37.0

Sprouts

E. evil present NAME OF KIDNEYS

Control, without treatment 16.4 5.2 013 0.05965 polyguanidine 16.0 42 10.0

E. other M ШНЕ ХНИ ШЕ. KI AIM 0.0196 -- 12.0 11.7 44.5

Herbicidal background, harness - 2 l/ha

Non-treatment control 00000697 105Ї1 0.05965 polyguanidine 6.3 17.3 22.3

E. zishshshannny ne-yay SHY AND SHY. NNI DOWN 0.0196 -- 62 18.8 18.5

Herbicidal background, dialne C - 2 l/ha Con- 0bgbeRNpolyguanidichud | 28 77777155 | 77777777 -1777717 11111 l1o4 troll, without processing Y Y 0.026 r-npolyanidin.d ОЙ 201 77771791 ЙИ

The sampling error does not exceed 595 of the mean values

The use of claimed inventions, the effectiveness and efficiency of agricultural technology will stimulate the growth and development of agriculture, especially with the wide use of grain crops, mainly wheat and corn, herbicides and their complexes. zy, to increase their phytoimmune potential, which in the end, it is expedient to patent these inventions, will ultimately cause an increase in the yield of the Russian Federation.

Computer layout M. Klyukin Signature Circulation 26 approx.

Ministry of Education and Science of Ukraine

State Department of Intellectual Property, str. Urytskogo, 45, Kyiv, MSP, 03680, Ukraine

SE "Ukrainian Institute of Industrial Property", str. Glazunova, 1, Kyiv - 42, 01601