TWI646508B - Equipment for detecting dna amplifying reaction - Google Patents

Abstract

A detection device for deoxyribonucleic acid (DNA) amplification reaction. The device includes a body, a track device, a mobile detection device, and a detection source. The body has an accommodation space for receiving the carrier tray of the experimental container. The track device is disposed on a sidewall of the body. The mobile detecting device is disposed on the track device and located in the accommodating space. The mobile detection device includes a plurality of focusing lens groups and a plurality of optical fibers. These fibers are connected one-to-one to the focusing lens group to project excitation light through the focusing lens group to the target container. The detection source includes an excitation source and an optical detection device. The excitation light source is used to provide the aforementioned excitation light to the optical fiber, and the optical detection device is configured to capture the fluorescent signal of the target container through the optical fiber.

Description

Deoxyribonucleic acid amplification reaction detection device

The invention relates to a detection device for a deoxyribonucleic acid (DNA) amplification reaction.

Fluorescence imaging techniques are often used to detect components in biological samples, such as blood, urine, or saliva samples of humans or animals, to find features of such samples in certain aspects (eg, diseases). Because of the small amount of DNA available, fluorescent imaging techniques often fail to produce effective test results. In order to increase the available amount of DNA, a reaction for amplifying a DNA fragment (hereinafter referred to as a DNA amplification reaction), such as a polymerase chain reaction (PCR) or a thermostatic nucleic acid amplification (isothermal amplification), is usually performed first. To increase the amount of DNA available, and then to excite the sample by laser light, so that the fluorescence reaction can be used to analyze the components in the sample.

It is an object of the present invention to provide a detection device for DNA amplification reaction which uses a mobile detection module to detect DNA to reduce the manufacturing cost of the detection device.

The DNA amplification reaction device comprises a body, a track device, a mobile detection device, and a detection source. The body has an accommodating space for accommodating the carrier tray, wherein the body includes a plurality of sidewalls to define the accommodating space described above. The track device is disposed on one of the side walls. The mobile detecting device is disposed on the track device and located in the accommodating space, wherein the mobile detecting device comprises a plurality of focusing lens groups and a plurality of optical fibers. The fibers are connected one-to-one to the focusing lens group and are used to transmit excitation light through the focusing lens group to a plurality of target containers in the experimental container. The detection source is connected to the mobile detection device. The detection source includes an excitation light source and an image capture device. The excitation source is used to provide excitation light to the fiber. The image capturing device is configured to capture an image of the target container through the optical fiber.

According to an embodiment of the invention, the DNA amplification reaction device further comprises a temperature control module for heating the experimental container.

According to still another embodiment of the present invention, the number of the focus lens group and the optical fiber module is a multiple of 4 or 4.

According to yet another embodiment of the invention, the excitation source is a laser source.

According to the foregoing objects, the DNA amplification reaction device comprises a body, a track device, a mobile detection device, and a detection source. The body has an accommodating space for accommodating the carrier tray, wherein the body includes a plurality of sidewalls to define the accommodating space described above. The track device is disposed on one of the side walls. The mobile detecting device is disposed on the track device and located in the accommodating space, wherein the mobile detecting device comprises a plurality of focusing lens groups and a plurality of optical fibers. The fibers are connected one-to-one to the focusing lens group and are used to transmit excitation light through a focusing lens group to one of the target containers in the experimental container. Detection source connection To the mobile detection device. The detection source includes a fiber selection module, an excitation source, and an optical detection device. The fiber selection module is configured to select a target fiber from the above fibers. The excitation light source is used to provide excitation light to the target fiber. The optical detecting device is configured to capture the excited fluorescent signal of the target container through the optical fiber.

In accordance with yet another embodiment of the present invention, the mobile detection device further includes a fiber optic connector for connecting the fiber to the fiber optic selection module.

According to still another embodiment of the present invention, the fiber optic selection module includes a mobile connector for engaging the fiber optic connector, and the mobile connector moves the fiber optic connector according to the external signal to selectively project the excitation light to One of the above optical fibers, which is the target optical fiber.

According to an embodiment of the invention, the DNA amplification reaction device further comprises a temperature control module for heating the experimental container.

According to still another embodiment of the present invention, the number of the focus lens group and the optical fiber module is a multiple of 4 or 4.

According to yet another embodiment of the invention, the excitation source is a laser source.

100‧‧‧Detection equipment for DNA amplification reaction

110‧‧‧ body

112‧‧‧ side wall

120‧‧‧Track device

130‧‧‧Mobile detection device

132a‧‧‧Fiber Optic Connectors

132b‧‧‧Fiber

134‧‧‧focus lens group

140‧‧‧Sliding cover

150‧‧‧Detection source

151‧‧‧Excitation source

152‧‧‧Distribution unit

153‧‧‧ zoom lens set

154‧‧‧Vision Module Unit

154a‧‧‧ imaging mirror

154b‧‧‧Optical inspection device

155‧‧‧Control unit

156‧‧‧Filter

160‧‧‧Up heating module

160H‧‧‧heating holes

170‧‧‧temperature control module

170H‧‧‧heating holes

350‧‧‧Detection source

352‧‧‧Fiber selection module

352a‧‧‧Mobile connector

352b‧‧‧ Connecting rod

354b‧‧‧Optical inspection device

ALA‧‧‧ coverage

CA‧‧‧ accommodating space

CAP‧‧‧Upper cover

LA‧‧‧Excited light

LS‧‧‧Fluorescence reaction

PR‧‧‧Receiving point

SP‧‧‧ Carrying tray

TU‧‧‧Experimental Container

For a more complete understanding of the embodiments and the advantages thereof, the following description is made with reference to the accompanying drawings, wherein: FIG. 1a is a schematic structural diagram of a detecting apparatus for DNA amplification reaction according to an embodiment of the present invention; 1B is a schematic diagram showing the excitation detecting device receiving the excitation light of the detection source according to an embodiment of the present invention; [FIG. 2a], [FIG. 2b] and [FIG. 2d] are diagrams according to an embodiment of the present invention. Schematic diagram of the detection of the experimental container by the mobile detecting device; [Fig. 2c] is a schematic structural view of the heating module according to an embodiment of the present invention; [Fig. 3] shows the optical fiber connector according to an embodiment of the present invention. FIG. 4a is a schematic view showing the structure of a detecting source according to another embodiment of the present invention; and FIG. 4b and FIG. 4c are side views showing a fiber optic connector according to another embodiment of the present invention. .

Embodiments of the invention are discussed in detail below. However, it will be appreciated that the embodiments provide many applicable concepts that can be implemented in a wide variety of specific content. The examples discussed and disclosed are illustrative only and are not intended to limit the scope of the invention.

Referring to FIG. 1a and FIG. 1b, FIG. 1a is a schematic structural diagram of a detecting apparatus 100 for DNA amplification reaction according to an embodiment of the present invention, and FIG. 1b is a schematic diagram of the mobile detecting apparatus 130 according to an embodiment of the present invention. A schematic diagram of the excitation light LA of the source 150 is detected. The DNA amplification reaction detecting apparatus 100 includes a body 110, a track device 120, a mobile detecting device 130, a slide cover 140, and a detection source 150. The body 110 has a sidewall 112, which is defined The space CA is taken out to accommodate the carrier disk SP. The carrier disk SP is used to carry an experimental container TU, such as a test tube containing a DNA sample. After the carrier disk SP is placed in the accommodating space CA, the slider 140 can close the accommodating space CA for DNA amplification reaction and DNA detection.

In the present embodiment, the carrier disk SP has 6×8 through holes for carrying 48 experimental containers TU, but the embodiment of the present invention is not limited thereto. In other embodiments of the invention, the carrier disk SP may have a through hole of a multiple of 4 or 4 to carry an experimental container that is a multiple of 4 or 4. For example, the carrier tray SP can have 12 x 8 through holes to carry 96 experimental container TUs.

The track device 120 is disposed on the side wall 112, and the mobile detecting device 130 is disposed on the track device 120 such that the mobile detecting device 130 can be moved through the track device 120 in the direction of the plane of the carrier disk SP. The mobile detection device 130 is coupled to the detection source 150 such that the detection source 150 transmits the experimental container TU through the mobile detection device 130.

As shown in FIG. 1b, the detection source 150 can be, for example, the device disclosed in the Patent No. I577484 of the Republic of China. Specifically, the detection source 150 includes an excitation light source 151, a beam splitting unit 152, a zoom lens group 153, a vision module unit 154, and a control unit 155. The vision module unit 154 includes an imaging lens set 154a and an image capturing device 154b. The imaging lens set 154a is disposed between the beam splitting unit 152 and the image capturing device 154b to facilitate light imaging, and the image capturing device 154b is configured to capture the image.

The excitation light source 151 is used to provide the excitation light LA, and the light splitting unit 152 is disposed on the conduction path of the excitation light LA. Thus, the excitation light LA can be transmitted to the variable zoom lens group 153 through the beam splitting unit 152. In this implementation In an example, the excitation light source 151 can be, for example, a laser light source, but embodiments of the invention are not limited thereto. The control unit 155 is electrically connected to the zoom lens group 153 to adjust the focal length of the zoom lens group 153 and project the excitation light LA to the optical fiber module 132.

In this embodiment, the fiber optic module 132 includes a fiber optic connector 132a and a plurality of fiber optics 132b, wherein the fiber optic connector 132a is used to connect the detection source 150 to enable the excitation light LA to be transmitted to the fiber 132b.

Please refer to FIG. 2a and FIG. 2b, which are schematic diagrams showing the detection of the experimental container TU by the mobile detecting device 130 according to an embodiment of the invention. The mobile detection device 130 includes the aforementioned fiber optic module 132 and a plurality of focusing lens groups 134. The fibers 132b of the fiber optic module 132 are connected one-to-one to the focusing lens group 134 to project excitation light LA to the experimental container TU to excite the sample therein. In this embodiment, since the carrier disk SP has 6×8 through holes for carrying 48 experimental containers TU, the number of the focusing lens group 134 and the optical fiber 132b are both designed to be 8, so that the mobile detecting device 130 can cover each test. A row of experimental vessels TU (ie 8 experimental vessels TU). However, embodiments of the invention are not limited thereto. In other embodiments of the invention, the number of focusing lens groups 134 and fibers 132b can be designed to be a multiple of 4 or 4.

In addition, the detection source 150 of the present embodiment includes a filter 156 disposed between the imaging lens group 154a and the image capturing device 154b. The filter 156 includes the excitation light LA and the fluorescent signal excited by the experimental container TU because the light entering the imaging lens group 154a is used. In this embodiment, the filter 156 is used to remove light other than the fluorescent signal to facilitate the image. The taking device 154b captures the fluorescent signal. In this embodiment, the filter 156 is allowed to allow a specific wave. An optical device through which long rays of light (e.g., fluorescent light) pass, but embodiments of the present invention are not limited thereto.

The detection device 100 for DNA amplification reaction of this embodiment also includes an upper heating module 160 and a temperature control module 170. The temperature control module 170 is disposed below the carrier disk SP to heat and control the temperature of the bottom of the experimental container TU to facilitate polymerase chain reaction (PCR) or thermostatic nucleic acid amplification reaction (isothermal) Amplification). In the present embodiment, the temperature control module 170 has a plurality of heating holes 170H whose outer shape matches the bottom of the experimental container TU, so that the heating hole 170H directly contacts the bottom of the experimental container TU for heating.

The upper heating module 160 is disposed between the mobile detecting device 130 and the carrying tray SP to insulate the experimental container TU. As shown in FIG. 2c, the upper heating module 160 has a plurality of strip-shaped heating holes 160H, and the outer shape is matched with the upper cover CAP of the experimental container TU, so that the upper heating module 160 can pass through the heating hole 160H and the experimental container TU. The upper cover CAP contacts to provide insulation. In this embodiment, since the carrier disk SP has 6×8 through holes for carrying 48 experimental containers TU, the upper heating module 160 is designed with 8 strips of heating holes 160H. In other embodiments of the invention, the upper heating module 160 can be combined with the mobile detection device 130, as shown in Figure 2d. The bottom of the mobile detecting device 130 has a heating hole 170H which can provide a heat insulating function to the experimental container TU when the mobile detecting device 130 performs the detecting.

As shown in FIG. 2b, when the mobile detecting device 130 detects the experimental container TU, the mobile detecting device 130 is configured according to the user. Moving to the top of the experimental container TU (hereinafter referred to as the target container) to be detected, the detection source 150 then emits the excitation light LA to the mobile detecting device 130, and transmits the excitation light through the focusing lens group 134 of the mobile detecting device 130. LA is projected onto the target container to excite the sample therein. Then, the fluorescent reaction LS of the target container can be transmitted back to the image capturing device 154b of the detecting source 150 through the optical fiber 132b of the mobile detecting device 130. In this way, the user can know the fluorescent reaction status of the target container.

Referring to FIG. 3, a side view of an optical fiber connector 132a having a plurality of receiving points PR corresponding to the optical fibers 132b in a one-to-one correspondence is illustrated in accordance with an embodiment of the present invention. When the detection source 150 emits the excitation light LA to the fiber optic connector 132a, the excitation light LA will cover all of the reception points PR. That is, the coverage area ALA formed by the excitation light LA projected onto the fiber connector 132a covers all the receiving points PR. As such, all of the fibers 132b receive excitation light. However, embodiments of the invention are not limited thereto. In other embodiments of the invention, the coverage of the excitation light LA will only include a portion of the reception point PR.

4a, FIG. 4b and FIG. 4c, FIG. 4a is a schematic structural view of a detection source 350 according to another embodiment of the present invention, and FIGS. 4b and 4c are diagrams showing a fiber optic connector according to another embodiment of the present invention. Side view of the 132a. The detection source 350 is similar to the detection source 150, but differs in that the detection source 350 replaces the image capture device 154b with the optical detection device 354b, and further includes a fiber optic selection module 352. The optical detecting device 354b may include an optical sensing element such as a photodiode or a photomultiplier (PMT) to read the fluorescent signal. However, this issue The embodiment of the invention is not limited thereto. In other embodiments of the present invention, the optical detecting device 354b may also be the image capturing device 154b described above. The fiber optic selection module 352 includes a mobile connector 352a, a link 352b, and a controller (not shown), wherein the mobile connector 352a is used to engage the fiber optic connector 132a, and the link 352b is provided according to the controller. The control signal moves the mobile connector 352a in the direction DR, and the fiber connector 132a thus coupled to the mobile connector 352a also moves in the direction DR.

In the present embodiment, the excitation light LA of the detection source 350 covers only the reception point PR of the portion of the optical fiber connector 132a. As shown in FIG. 4b, the detection source 350 of the present embodiment uses an excitation light source with a small power. Therefore, the coverage ALA of the excitation light LA only includes one receiving point PR of the optical fiber connector 132a. By moving the fiber optic connector 132a by controlling the mobile connector 352a, the coverage ALA of the excitation light LA can be moved from one receiving point PR to another receiving point PR, as shown in Figure 4c. Thus, in this embodiment, the detection of DNA can be performed using a less powerful excitation light source.

As apparent from the above description, the detection device for the DNA amplification reaction of the embodiment of the present invention uses a mobile detection device for DNA detection. Compared with the conventional detecting device, since the detecting device of the embodiment of the present invention detects only the experimental container of the upper portion of the carrying disk at a time, an excitation light source with a smaller power can be used. As such, the detecting device of the embodiment of the present invention has a relatively low cost.

Although the present invention has been disclosed in the above embodiments, it is not intended to limit the present invention, and any one of ordinary skill in the art can make some changes and refinements without departing from the spirit and scope of the present invention. The scope of the invention is defined by the scope of the appended claims.

Claims (10)

A detection device for deoxyribonucleic acid (DNA) amplification reaction for detecting a plurality of experimental containers on a carrier tray, wherein the detection device for the DNA amplification reaction comprises: a body having an accommodation a space for accommodating the carrier, wherein the body includes a plurality of sidewalls to define the accommodating space; a track device disposed on one of the sidewalls; and a mobile detecting device disposed on the track device Located in the accommodating space, wherein the mobile detecting device comprises: a plurality of focusing lens groups; and a plurality of optical fibers connected to the focusing lens groups in a one-to-one manner, wherein the optical fibers are used to transmit the focusing lens groups An excitation light is projected onto the plurality of target containers in the experimental containers; a detection source is coupled to the mobile detection device, wherein the detection source comprises: an excitation light source for providing the excitation light to the optical fibers And an image capturing device for capturing images of the target containers through the optical fibers. The detection device for DNA amplification reaction according to claim 1, further comprising a temperature control module for heating the experimental containers. The apparatus for detecting a DNA amplification reaction according to claim 1, wherein the number of the focusing lens group and the plurality of optical fiber modules is a multiple of 4 or 4. The apparatus for detecting a DNA amplification reaction according to claim 1, wherein the excitation light source is a laser light source. A detection device for deoxyribonucleic acid (DNA) amplification reaction for detecting a plurality of experimental containers on a carrier tray, wherein the detection device for the DNA amplification reaction comprises: a body for providing a The accommodating space is configured to receive the carrier, wherein the body includes a plurality of sidewalls to define the accommodating space; a track device is disposed on one of the sidewalls; and a mobile detecting device is disposed on the track device And in the accommodating space, wherein the mobile detecting device comprises: a plurality of focusing lens groups; a plurality of optical fibers are connected to the focusing lens groups in a one-to-one manner, wherein each of the optical fibers is used to transmit the optical fibers One of the focusing lens groups projects an excitation light to one of the experimental containers; a detection source is coupled to the mobile detection device, wherein the detection source includes: a fiber optic selection module for Selecting a target fiber from the fibers; An excitation light source for providing the excitation light to the target optical fiber; and an optical detecting device for capturing the fluorescent signal excited by the target container through the target optical fiber. The apparatus for detecting a DNA amplification reaction according to claim 5, wherein the mobile detecting device further comprises a fiber optic connector for connecting the optical fibers to the fiber optic selection module. The apparatus for detecting a DNA amplification reaction according to claim 6, wherein the fiber selection module comprises a mobile connector for engaging the fiber connector, and the mobile connector moves the signal according to an external signal. a fiber optic connector for selectively projecting the excitation light to one of the fibers, the one of the fibers being the target fiber. The detecting device for DNA amplification reaction according to claim 5, further comprising a temperature control module for heating the experimental containers. The apparatus for detecting a DNA amplification reaction according to claim 5, wherein the number of the focusing lens group and the plurality of optical fiber modules is a multiple of 4 or 4. The apparatus for detecting a DNA amplification reaction according to claim 5, wherein the excitation light source is a laser light source.