KR20210029449A - Small sized diagnostics system using isothermal amplification - Google Patents

Abstract

The present invention relates to a compact diagnosis system using isothermal amplification. Particularly, the compact diagnosis system includes: a heating block having at least one receiving groove on the top surface thereof; at least one container coupled to the receiving groove and configured to receive a sample; a heater disposed in the heating block and configured to heat the container isothermally; a fluorescence analyzer unit which includes a light emitting module disposed in such a manner that it may be spaced apart from the heating block and configured to supply light toward the container, and an optical module disposed in such a manner that it may be spaced apart from the heating block and configured to detect the fluorescence caused by the amplification reaction of the sample in the container; and a conveying unit which includes a guide unit formed at one side of the heating block in the longitudinal direction of the heating block having at least one receiving groove, and a driving unit configured to convey the light emitting module and optical module along the guide unit.

Description

Small sized diagnostics system using isothermal amplification}

The present invention relates to a small diagnostic system using isothermal amplification, and more particularly, to a small diagnostic system for an isothermal amplification reaction for gene amplification capable of detecting fluorescence in real time through a gene amplification reaction.

Conventional acute viral molecular diagnostic sensor technology uses a realtime-polymerase chain reaction (RT-PCR) device to control the temperature required for the gene amplification reaction of a molecular diagnostic reagent with a thermal block and optically detect changes in fluorescence according to the amplification reaction. It consists of a structure that is read by a sensor.

However, since RT-PCR equipment is used, it is not suitable to be used as a device that can be quickly analyzed in the field by relying on laboratory equipment in a laboratory.

In addition, the expensive component that determines the cost of the diagnostic device is a fluorescence measurement unit that includes a bandpass (BP) filter.Since a complex optical module is used to detect fluorescence, it is difficult to reduce the price of the isothermal amplified fluorescence measurement device and it is difficult to reduce the size. There are drawbacks.

On the other hand, Patent Publication No. 2019-0063556 (name of the invention: molecular diagnosis system) has the advantage of obtaining accurate results by arranging the light emitting module and the optical module at an angle to the container so that light does not interfere with each other. As the module and the optical module are arranged symmetrically, there is a problem in miniaturization and low cost implementation as well.

Republic of Korea Patent Publication No. 2019-0063556 (2019.06.10)

Accordingly, an object of the present invention is to provide a small and low-cost diagnostic system for an isothermal amplification reaction for amplifying a gene capable of detecting fluorescence in real time through a gene amplification reaction.

A diagnostic system according to an embodiment of the present invention for achieving the above object includes: a heating block having one or more receiving grooves formed on an upper surface thereof; At least one container coupled to the receiving groove and accommodating a sample; A heater disposed in the heating block to heat the container isothermally; A fluorescence measurement unit having a light emitting module disposed to be spaced apart from the heating block and providing light toward the container, and an optical module disposed to be spaced apart from the heating block to detect fluorescence due to an amplification reaction of a sample in the container; And a guide part formed in a longitudinal direction of the heating block in which the one or more receiving grooves are formed on one side of the heating block, and a transfer part including a driving part for transferring the light emitting module and the optical module along the guide part. .

In addition, in the diagnostic system according to the present invention, the light emitting module is disposed on the side of the container to provide light toward the side of the container, and the optical module is disposed on the lower side of the container, It is characterized by detecting fluorescence due to the amplification reaction of the sample.

In addition, in the diagnostic system according to the present invention, the fluorescence measurement unit may be integrally combined with the light emitting module and the optical module.

In addition, in the diagnostic system according to the present invention, the heating block has a light source incident hole through which a light source is incident from the light emitting module on a side thereof, and fluorescence due to an amplification reaction of the sample in the container is transmitted to the optical module on a lower surface thereof. It may be that the emitting fluorescence emission hole is formed.

Further, in the diagnostic system according to the present invention, the light emitting module includes: a light source for providing light; A light source filter disposed at a rear end of the light source in a light transmission direction; And a lens disposed at a rear end of the light source filter, wherein the optical module includes: a photo diode (PD) configured to detect fluorescence generated in the container; A fluorescent filter disposed in front of the photodiode in a light receiving direction; And a lens disposed in front of the fluorescent filter.

In addition, in the diagnostic system according to the present invention, the light emitting module and the substrate part electrically connected to the optical module; And a terminal connected to the substrate to enable wired and wireless communication.

In addition, in the diagnostic system according to the present invention, the heating block includes at least one receiving groove arranged in two rows in a longitudinal direction, and the transfer unit includes a first light emitting module and a first light emitting module in the longitudinal direction of the receiving groove of the first row of the heating block. A first transfer unit having a first driving unit transferring the first optical module; And a second transfer unit including a second driving unit for transferring the second light emitting module and the second optical module in the longitudinal direction of the receiving groove of the second row of the heating block.

A diagnosis system according to another embodiment of the present invention includes: a heating block having one or more receiving grooves formed on an upper surface thereof; At least one container coupled to the receiving groove and accommodating a sample; A heater disposed in the heating block to heat the container isothermally; A light emitting module that provides light toward the container and includes a light source filter; An optical module that detects fluorescence due to the amplification reaction of the sample in the container and includes a fluorescence filter; And a guide part formed on one side of the heating block in the longitudinal direction of the heating block, and a transfer part including a driving part for transferring the light emitting module and the optical module along the guide part, including, and a light source filter of the light emitting module It characterized in that the fluorescent filter of the optical module is arranged at a right angle with respect to the sample.

Further, in the diagnostic system according to the present invention, the light emitting module includes: a light source for providing light; And a lens disposed at a rear end of the light source filter, wherein the optical module includes: a photo diode configured to detect fluorescence generated from the container; It may further include a lens disposed in front of the fluorescent filter.

According to the present invention, by transferring only one light emitting module and an optical module to one or more sample containers in a heating block, real-time fluorescence measurement is possible, thereby reducing the size and cost of a diagnostic system.

In addition, by integrating the light emitting module and the optical module and transferring them through a guide of the transfer unit, there is an advantage in that each component for diagnosis can be compactly arranged in the housing.

In addition, since the light source axis and the fluorescence emission axis are 90 degrees with respect to the container so that light does not interfere with each other between the light emitting module and the optical module, there is an advantage of obtaining a more accurate result value by preventing the light source from being directly incident on the measurement unit.

In addition, by managing the diagnosis system according to the present invention through a control app through a terminal, it is excellent in usability, it is easy to implement a point-of-care testing (POCT) type molecular diagnosis system, and the system can be easily managed from outside. There is an advantage.

1 is a schematic diagram showing a small diagnostic system using isothermal amplification including a heating block having eight containers, an integrated light emitting module and an optical module, and a transfer unit according to an embodiment of the present invention.
FIG. 2 is a schematic diagram showing a fluorescence measurement unit including a light emitting module and an optical module and having an optical axis vertically arranged to measure a change in fluorescence of a sample contained in a container according to an embodiment of the present invention.
3 is a light-emitting module disposed on the side of a container according to an embodiment of the present invention to allow light to be incident to the side, and an optical module is placed at the bottom of the container to arrange the light-emitting module and the optical axis at 90 degrees to improve light measurement efficiency It is a cross-sectional view showing the optical path structure of the raised heating block.
4 is a photograph showing an operation example of measuring a change in fluorescence in a container while moving a fluorescence measuring unit according to an embodiment of the present invention.
5 is a photograph showing a diagnostic system including a transfer unit for transferring a fluorescence measurement unit according to an embodiment of the present invention.
6 is a fluorescence measurement result according to an embodiment of the present invention.
7 is a conceptual diagram illustrating a configuration in which a fluorescence measurement unit moves through a motor to extract fluorescence values of each sample according to an exemplary embodiment of the present invention.
8 is a graph illustrating an algorithm for extracting a fluorescence value of each sample through a constant velocity movement of a fluorescence measurement unit according to an embodiment of the present invention.
9 is a graph illustrating an algorithm for extracting a fluorescence value emitted from a location of each sample container according to an embodiment of the present invention.
10 is a measurement result of processing an amplification time point by outputting a change in fluorescence intensity of a sample contained in a container according to an embodiment of the present invention.

Since the present invention can apply various transformations and have various embodiments, specific embodiments will be described in detail in the detailed description. However, this is not intended to limit the present invention to a specific embodiment, it should be understood to include all conversions, equivalents, and substitutes included in the spirit and scope of the present invention. In describing the present invention, when it is determined that a detailed description of a related known technology may obscure the subject matter of the present invention, a detailed description thereof will be omitted.

The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In the present application, terms such as "comprise" or "have" are intended to designate the presence of features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, but one or more other features. It is to be understood that the presence or addition of elements or numbers, steps, actions, components, parts, or combinations thereof does not preclude in advance.

Terms such as first and second may be used to describe various elements, but the elements should not be limited by the terms. The above terms are used only for the purpose of distinguishing one component from another component.

Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. It is apparent to those skilled in the art that the present invention can be embodied in other specific forms without departing from the spirit and essential features of the present invention.

1 is a schematic diagram showing a small diagnostic system using isothermal amplification including a heating block having eight containers, an integrated light emitting module and an optical module, and a transfer unit according to an embodiment of the present invention. It is a schematic diagram showing a fluorescence measurement unit including a light emitting module and an optical module and having an optical axis vertically arranged to measure fluorescence change of a sample contained in a container according to an exemplary embodiment of the present invention. 3 is a light-emitting module disposed on the side of a container according to an embodiment of the present invention to allow light to enter the side, and an optical module is placed at the bottom of the container to arrange the light-emitting module and the optical axis at 90 degrees to improve light measurement efficiency. It is a cross-sectional view showing the optical path structure of the raised heating block.

1 to 3, a diagnostic system according to an embodiment of the present invention includes a heating block 10, one or more containers 20, a heater (not shown), a fluorescence measurement unit 30, and a transfer unit 40. Includes.

The heating block 10 has one or more receiving grooves 22 formed on the upper surface thereof.

The heating block 10 performs isothermal heating and temperature maintenance functions required for isothermal amplification of genes, and is insulated from the outside to maintain the temperature required for isothermal amplification with small energy consumption.

One or more containers 20 are coupled to correspond to the one or more receiving grooves 22 and receive a sample.

A container mounting part for loading the sample container 20 may be provided inside the heating block 10. The receiving groove 22 is recessed downward from the upper surface of the heating block 10, so that the container 20 may be coupled from the upper side.

A heater (not shown) is disposed in the heating block 10 to heat the container 20 isothermally. The heating block 10 may further include a temperature sensor along with a heater therein. Through this, it is possible to maintain an isothermal temperature, and an appropriate temperature environment is provided so that an isothermal amplification reaction can occur in the container 20.

The fluorescence measurement unit 30 includes a light emitting module 50 and an optical module 60.

When a target gene is present in the reagent in the container 20, a change in the fluorescence signal occurs as the amplification reaction proceeds. In order to measure this in real time, a fluorescence measurement unit that measures fluorescence is required.

The present invention can measure the change in fluorescence in each container 20 with only one fluorescence measuring unit 30 with respect to the heating block 10 capable of simultaneously inserting one or more sample containers 20.

The light emitting module 50 is disposed to be spaced apart from the heating block 10 and disposed on the side of the container 20 to provide light toward the side of the container 20.

The light emitting module 50 includes a light source providing light, a light source filter disposed at a rear end of the light source in a light transmission direction, and a lens disposed at a rear end of the light source filter.

The light source provides light for an amplification reaction of the sample in the container 20. For example, the light source may be a Light Emitting Diode (LED).

The optical module 60 is disposed to be spaced apart from the heating block 10 and disposed on the lower surface of the container 20 to detect fluorescence due to an amplification reaction of the sample in the container 20.

The optical module 60 includes a photodiode for detecting fluorescence generated in the container 20, a fluorescent filter disposed at a front end of the photodiode in a light receiving direction, and a lens disposed at the front end of the fluorescent filter Includes.

The photodiode detects fluorescence of the container 20 generated as a result of an amplification reaction. When fluorescence is generated as a result of isothermal heating of the container 20, the photodiode may detect it.

In one embodiment of the present invention, the fluorescence measurement unit 30 may be composed of LED/PD. The wavelength of the light source is suitably configured according to the characteristics of the fluorescent material in the isothermal amplification reaction. For example, when using Cybergreen as a fluorescent material, the LED wavelength is 490nm, and the filter uses a band pass filter that passes 490nm and 520nm to the light source side and the PD side, respectively. The optical system is provided at the side and bottom with the container 20 interposed so that the light source does not directly enter the PD, so that only the fluorescence resulting from the isothermal amplification reaction is collected and sent to the PD to improve the fluorescence measurement efficiency.

All of the lenses are refractive index distribution lenses, and may be, for example, a rod lens. In order to increase the collection efficiency of fluorescence, a rod lens may be used as an optical guide and may be additionally provided in a hole at the bottom, and a BP filter and a PD for fluorescence may be provided at the bottom thereof.

2 shows the structure of the fluorescence measurement unit 30, and the light source filter of the light emitting module 50 and the fluorescence filter of the optical module 60 are arranged at 90 degrees with respect to the sample.

3 shows a cross-sectional view of a heating block 10 having a micro-container 20, a light source incident hole, and a fluorescence emission hole. 3, in the heating block 10 of the present invention, a light source incident hole through which a light source is incident from the light emitting module 50 is formed on the side, and fluorescence due to the amplification reaction of the sample in the container 20 is formed on the lower side. A fluorescence emission hole for radiating to the optical module 60 is formed. That is, in order to measure the fluorescence change, a light source incident hole and a fluorescence emission hole are provided in the heating block, and since the light source axis and the fluorescence emission axis are 90 degrees, an optical path is provided so that the light source does not enter the measurement unit directly.

Accordingly, in the present invention, more accurate results can be obtained by preventing light from interfering between the light emitting module 50 and the optical module 60 and allowing the light source to not be directly incident on the measurement unit.

Preferably, the fluorescence measurement unit 30 may be a combination of the light emitting module 50 and the optical module 60 integrally. By integrating the light-emitting module 50 and the optical module 60 and transferring them through a guide of the transfer unit 40, each component for diagnosis can be compactly disposed in the housing.

The transfer part 40 includes a guide part formed in the longitudinal direction of the heating block 10 in which the one or more receiving grooves 22 are formed on one side of the heating block 10, and the light-emitting module 50 along the guide part. And a driving unit for transferring the optical module 60.

4 is a photograph showing an operation example of measuring a change in fluorescence in the container 20 while moving the fluorescence measurement unit 30 according to an embodiment of the present invention, and FIG. 5 is a fluorescence measurement according to an embodiment of the present invention. This is a photograph showing a diagnosis system including a transfer unit 40 that transfers the unit 30.

4 to 5, the driving unit for transferring the light emitting module 50 and the optical module 60 of the present invention may include a linear motor, but is not limited thereto. In the present invention, as an example, it is possible to measure eight channels (8 containers) in a heating block while moving one fluorescence measuring unit 30 including the light emitting module 50 and the optical module 60 with a linear motor. .

Therefore, in the present invention, real-time fluorescence measurement is possible by transferring only one fluorescence measurement unit 30 including the light emitting module 50 and the optical module 60 to one or more sample containers 20 in the heating block 10. By doing so, there is an advantage in that the diagnostic system can be miniaturized and the cost can be reduced.

For example, in the heating block 10, one fluorescence measurement unit 30 including the light emitting module 50 and the optical module 60 is moved with respect to the heating block 10 including the eight containers 20. It is possible to lower the cost of the diagnostic system by having the canister 20 be measured.

On the other hand, the present invention is a substrate portion electrically connected to the light emitting module 50 and the optical module 60; And a terminal connected to the substrate to enable wired and wireless communication.

In addition, the diagnostic system of the present invention may further include a control unit including a GUI that manages each component as a whole and displays a result. The control unit including the GUI can be implemented with Android OS-based software or an embedded system such as Raspberry Pi. Through this, it is possible to configure a system that controls isothermal and fluorescence measurements with an application program of a mobile device and displays the status and results.

The present invention has the advantage of being able to manage the diagnosis system of the present invention by interlocking it with a control app through a terminal, so that it is excellent in usability, it is easy to implement a POCT-type molecular diagnosis system, and can easily manage the system from outside. In addition, it can be used in a field diagnosis virus measurement system based on a mobile device.

In another embodiment according to the present invention, in the heating block 10, one or more receiving grooves 22 may be arranged in two rows in the longitudinal direction. In this case, the transfer unit 40 includes a first transfer unit having a first driving unit for transferring the first light emitting module and the first optical module in the longitudinal direction of the receiving groove 22 of the first row of the heating block 10; And a second transfer unit including a second driving unit for transferring the second light emitting module and the second optical module in the longitudinal direction of the receiving grooves 22 in the second row of the heating block 10.

Here, it may be preferable that the heating block 10 has a heater in the center of the heating block 10 and each of the eight containers 20 in two rows is provided on both sides. In addition, since the fluorescence measurement unit has an'L'-type structure, isothermal amplification that can measure 16 containers (8+8) while transferring the 2 fluorescence measurement units 30 equipped with a symmetric (mirror symmetric) structure of the'L'. It is also possible to expand to the device.

A diagnostic system according to another embodiment of the present invention includes a heating block 10 in which one or more receiving grooves 22 are formed on an upper surface; One or more containers 20 coupled to the receiving groove 22 and accommodating a sample; A heater disposed in the heating block 10 to heat the container 20 isothermally; A light emitting module 50 that provides light toward the container 20 and includes a light source filter; An optical module 60 that detects fluorescence due to the amplification reaction of the sample in the container 20 and includes a fluorescence filter; And a guide part formed on one side of the heating block 10 in the longitudinal direction of the heating block 10, and a driving part for transferring the light emitting module 50 and the optical module 60 along the guide part. (40); Including, characterized in that the light source filter of the light emitting module 50 and the fluorescent filter of the optical module 60 are disposed at a right angle with respect to the sample.

The light emitting module 50 further includes a light source for providing light, and a lens disposed at a rear end of the light source filter. In addition, the optical module 60 further includes a photodiode for sensing fluorescence generated in the container 20, and a lens disposed at a front end of the fluorescence filter.

6 is a fluorescence measurement result according to an embodiment of the present invention.

Referring to FIG. 6, FIG. 6 shows an example of a result of measuring six tubes (containers) once while transporting the fluorescence measurement unit 30. As shown in FIG. In proportion to the amount of the fluorescent sample, the PD output signal appears large, and by observing this over time, it can be confirmed that fluorescence amplification occurs.

7 to 9 illustrate a method of reading a fluorescence value of a diagnostic system according to an embodiment of the present invention.

7 is a conceptual diagram illustrating a configuration in which a fluorescence measurement unit moves through a motor to extract fluorescence values of each sample according to an exemplary embodiment of the present invention.

Referring to FIG. 7, FIG. 7 extracts the fluorescence value of each sample by moving a fluorescence measurement unit composed of a light emitting module and an optical module through a motor, and outputs the trend of the fluorescence change value of each sample as a graph in a 1-minute cycle. It shows the amplification process.

The motor can be controlled by known firmware. Here, the motor is driven by GPIO (general-purpose input/output) control, the motor power is 4v, the ADC (analog-digital converter) sampling rate is 200SPS, and the ADC resolution is 16 bits ( 0-65536), the elapsed time for the first measurement is 2.65 seconds.

8 is a graph illustrating an algorithm for extracting a fluorescence value of each sample through a constant velocity movement of a fluorescence measurement unit according to an embodiment of the present invention.

9 is a graph illustrating an algorithm for extracting a fluorescence value emitted from a location of each sample container according to an embodiment of the present invention.

Referring to FIG. 9, FIG. 9 shows the position of the peak of the signal in order to extract the fluorescence value emitted from the position of each sample tube, and the maximum or maximum fluorescence value at each position index. It is a graph explaining an algorithm that takes the average of values and reads them as fluorescence values. The fluorescence amplification response graph is drawn by outputting the change in the time period of the fluorescence value in a 1-minute period as a graph.

10 is a measurement result of processing an amplification time point by outputting a change in fluorescence intensity of a sample contained in a container according to an embodiment of the present invention. The measurement result is to monitor the isothermal amplification process by reciprocating the linear motor one or more times, measuring and storing the fluorescence intensity of each tube (container), and outputting the change in fluorescence intensity of each tube sample at a 1-minute interval. This is the measurement result of the fluorescence data analysis system processing the amplification time point.

Referring to FIG. 10, FIG. 10 is a graph showing that a sample in three containers (tubes) has a large change in fluorescence, a small change in fluorescence, and no change in fluorescence. This is an example of output.

According to the present invention, it is possible to reduce the manufacturing cost of the device by simply measuring isothermal fluorescence amplification by using one fluorescence measuring unit 30 for eight samples. That is, the expensive component that determines the cost of the diagnostic device is a fluorescence measurement unit including a bandpass (BP) filter. In the conventional case, a complex optical module is used to detect fluorescence, thus reducing the cost of an isothermal amplified fluorescence measurement device. There was a drawback that was difficult and difficult to downsize. In the present invention, since one fluorescence measuring unit 30 can monitor eight sample containers at once, there is an advantage in that a low-cost small device can be manufactured. Therefore, by transferring only one light-emitting module 50 and optical module 60 to one or more sample containers 20 in the heating block 10, real-time fluorescence measurement is possible, thereby reducing the size and cost of the diagnostic system. .

In addition, by integrating the light emitting module 50 and the optical module 60 and transferring them through the guide of the transfer unit 40, there is an advantage in that each component for diagnosis can be compactly disposed in the housing.

In addition, the light source axis and the fluorescence emission axis of the container 20 are 90 degrees so that light does not interfere with each other between the light emitting module 50 and the optical module 60, so that the light source is not directly incident on the measurement unit, thereby providing more accurate results. Can be obtained, thereby improving the fluorescence measurement sensitivity.

In addition, it is easy to measure fluorescence by amplification reaction of the analyzed sample compared to the control (negative control).

In addition, since the diagnosis system according to the present invention is managed by interlocking with a control app through a terminal, it is excellent in usability, it is easy to implement a POCT-type molecular diagnosis system, and it is possible to easily manage the system from outside.

Meanwhile, the above detailed description should not be construed as restrictive in all respects and should be considered as illustrative. The scope of the present invention should be determined by reasonable interpretation of the appended claims, and all changes within the equivalent scope of the present invention are included in the scope of the present invention.

10: heating block 20: container
22: container receiving groove
30: fluorescence measurement unit 40: transfer unit
50: light-emitting module 60: optical module

Claims (12)

A heating block in which at least one receiving groove is formed on an upper surface;
At least one container coupled to the receiving groove and accommodating a sample;
A heater disposed in the heating block to heat the container isothermally;
A fluorescence measurement unit having a light emitting module disposed to be spaced apart from the heating block and providing light toward the container, and an optical module disposed to be spaced apart from the heating block to detect fluorescence due to an amplification reaction of a sample in the container; And
A transfer part including a guide part formed in a longitudinal direction of the heating block in which the at least one receiving groove is formed on one side of the heating block, and a driving part for transferring the light emitting module and the optical module along the guide part;
Diagnostic system comprising a.
The method of claim 1,
The light emitting module is disposed on the side of the container to provide light toward the side of the container,
The optical module is disposed on a lower surface of the container, and detects fluorescence due to an amplification reaction of a sample in the container.
The method of claim 1,
The fluorescence measurement unit, the light-emitting module and the optical module is integrally coupled to the diagnostic system.
The method of claim 1, wherein the heating block,
A light source incident hole through which a light source is incident from the light emitting module is formed on the side,
A diagnostic system, wherein a fluorescence emission hole for emitting fluorescence due to an amplification reaction of the sample in the container is formed on a lower surface thereof to the optical module.
The method of claim 1,
The light emitting module,
A light source providing light;
A light source filter disposed at a rear end of the light source in a light transmission direction; And
Including a lens disposed at the rear end of the light source filter,
The optical module,
A photodiode for sensing fluorescence generated in the container;
A fluorescent filter disposed in front of the photodiode in a light receiving direction; And
And a lens disposed at the front end of the fluorescent filter.
The method of claim 1,
A substrate part electrically connected to the light emitting module and the optical module; And
Diagnosis system further comprising a terminal connected to the substrate to enable wired and wireless communication.
The method of claim 1,
In the heating block, at least one receiving groove is arranged in two rows in the longitudinal direction,
The transfer unit,
A first transfer unit including a first driving unit for transferring the first light emitting module and the first optical module in the longitudinal direction of the receiving groove in the first row of the heating block; And
And a second transfer unit including a second driving unit for transferring the second light emitting module and the second optical module in the longitudinal direction of the receiving groove of the second row of the heating block.
A heating block in which at least one receiving groove is formed on an upper surface;
At least one container coupled to the receiving groove and accommodating a sample;
A heater disposed in the heating block to heat the container isothermally;
A light emitting module that provides light toward the container and includes a light source filter;
An optical module that detects fluorescence due to the amplification reaction of the sample in the container and includes a fluorescence filter; And
Including; a guide portion formed on one side of the heating block in the longitudinal direction of the heating block, and a transfer portion including a driving portion for transferring the light emitting module and the optical module along the guide portion; and
The diagnostic system, characterized in that the light source filter of the light emitting module and the fluorescent filter of the optical module are disposed at right angles with respect to the sample.
The method of claim 8,
The light emitting module and the optical module are integrally combined,
The light emitting module is disposed on the side of the container to provide light toward the side of the container,
The optical module is disposed on a lower surface of the container, and detects fluorescence due to an amplification reaction of a sample in the container.
The method of claim 8, wherein the heating block,
A light source incident hole through which a light source is incident from the light emitting module is formed on the side,
A diagnostic system, wherein a fluorescence emission hole for emitting fluorescence due to an amplification reaction of the sample in the container is formed on a lower surface thereof to the optical module.
The method of claim 8,
The light emitting module,
A light source providing light; And
Further comprising a lens disposed at the rear end of the light source filter,
The optical module,
A photodiode for sensing fluorescence generated in the container; And
The diagnostic system further comprising a lens disposed at the front end of the fluorescent filter.
The method of claim 8,
A substrate part electrically connected to the light emitting module and the optical module; And
Diagnosis system further comprising a terminal connected to the substrate to enable wired and wireless communication.

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