200944244 九、發明說明： 【發明所屬之技術領域】 本發明係關於一種無患子去頭皮屑活性物質其製備方法及 含彼組成物，特別是指一種將無患子果實假種皮經過乙酸乙酯 萃取而得之去頭皮屑活性物質，以及該活性物質之製備方法。 【先前技術】 參 ❹ 自古以來，無患子s聊如厂仍幻.）即被普遍應用於 清潔去污之用途，依據本草綱目記載，無患子屬木部，其子皮（即 核外肉—假種皮）微苦、平、有小毒；並強調無患子之子皮（假種 皮）具有「去風明目」之效用，取無患子皮、皂角、胡餅、草富 蒲’同捶碎，加漿水調作彈子大，取以泡湯洗頭，能去風明目； 無患子亦具有「洗面去斑」之效果，將無患子皮搗爛，加白麵 和為丸’每日取以洗面，去垢及斑甚效等功能。 早年台灣全省不論平地或山區，遍地都是無患子樹’民眾 撿拾其果實，搗碎後即可t成肥皂使用；但合成清㈣問世後， 無患子便乏人問津；然而，過度使用合成清潔劑卻造成環境污 染等問題。近年來，隨著環保音 思戒的如幵，而且化學產品之副 作用與毒性屢被發現，一般消費者普遍興起崇尚自然產品之風 潮’因此’以無患子所製成的天然界面活性劑及其相關產品受 到高度重視。 無患子假種皮化學成分複雜 男職頭、酵素、阿拉伯膠、 及皂苷皂素元等，其中以所含的 县哥皂素（saponins)最受重 6 200944244 視，此成分除了為人所知的清潔功用外，還有許多生物活性。 由方；無患子果貫化學成分極其複雜，以水萃液所製成之產品為 例’系有產品穩定性不佳、色澤易變黑，以及會產酸及臭味等 1 由此可見，上述習用無患子清潔用產品仍有諸多缺失， 實非一良善之設計者，而亟待加以改良。 俗稱之頭皮屑其實是一種發生於頭皮的輕微脂漏性皮膚 炎；許多研究指出，橢圓形皮屑芽胞菌, ®舊名尸"·^°柳厂⑽⑽在脂漏性皮膚炎的致病機轉裡扮演 著重要的角色；該菌是一種普遍存在於人體皮膚表面的酵母樣 真菌，其分佈最多的地方亦是脂漏部位，若在有頭皮屑的頭皮 上偵測橢圓形皮屑芽胞菌，其數量會比正常人頭皮多；在市面 上月b有效治療頭皮屑的洗髮精裡，或多或少都含有可抑制橢圓 形皮屑芽胞菌之成份，足見橢圓形皮屑芽胞菌在頭皮屑的形成 過程中’必然扮演著某種重要的角色。 0 然而’本案發明人等，歷經兩年之系列探討與測試之結果 顯示’直接抑制皮屑芽胞菌之生長僅能獲得治標之效果，經過 —段時間後’皮屑芽胞菌可能對抑制物已產生抗性，而又再次 出現於頭皮屑患者之頭皮上；若要根治頭皮屑症狀，理應調適 碩皮表層之介面活性狀態，亦即形成比較不易導致脂漏性皮膚 炎之生理環境’乃是減少頭皮屑產生之治本之道。 本案發明人鑑於上述習用無患子清潔用產品以及習用去頭 200944244 皮屑洗髮精所衍生的各項缺點，乃亟思加以改良創新，並經多 年苦心孤詣潛心研究後，終於成功研發完成本件無患子去頭皮 屑活性物質其製備方法及含彼組成物。 【發明内容】 本發明之目的係在於提供一種無患子去頭皮屑活性物質及 含彼之組成物，以作為可去除頭皮屑之洗髮清潔用品。 本电明之次一目的即在於提供一種無患子去頭皮屑活性物 Φ 質之製備方法，以產生穩定性佳、不易變色及變質之無患子去 頭皮屑活性物質。 為達成上述發明目的，本案發明人係以無患子果實成熟期 間，不同成熟度具有不同化學組成而且不同成分有其特定功能 之概念，經由長時間之取樣檢測深入探討，針對較低極性之皂 素凡為主要萃取對象，研發以青果（翠綠果實階段）為原料，取 無患子青果之假種皮經適當乾燥粉碎之前處理後，再以乙酸乙 ® s旨進行萃取，並將該萃取液進—步減壓濃縮乾燥製成活性區分 物粕末產。σ，戎產品可調節頭皮表層介面活性形成頭皮屑較不 易產生之環境。 因此，可達成上述發明目的之無患子去頭皮屑活性物質之 製備方法，包括下列步驟： 步驟1 :將新鮮之無患子青果於45至55°c下進行2至4 天之乾燥處理； 200944244 步驟2:破碎乾燥之盔 、， ^ … 月果’亚分離其種子及假種皮； v驟3 ·以乙酸乙酯溶劑萃 卒取s亥假種皮，假種皮與乙酸乙酯 冷诏之比例為1/5至1/2〇 、 里體積比），卒取後，以過濾或離 心，方法分離萃取液及固體物；以及 1步驟4:以減壓濃縮法去除該萃取液所含之乙酸乙醋溶劑， 得到之萃取物即為無患子去頭皮屬活性物質。200944244 IX. Description of the Invention: [Technical Field] The present invention relates to a method for preparing a dandruff-free active substance and a composition thereof, and more particularly to a method for extracting a fruitless aril of ethyl acetate from ethyl acetate. An anti-dandruff active, and a method of preparing the active. [Prior Art] Since the ancient times, the sorrowful stalks are still used in the cleansing and decontamination. According to the Compendium of the Compendium, the sorghum is a stalk, and its sub-skin (ie, extra-core meat - aril ) slightly bitter, flat, and slightly poisonous; and emphasize that the skin of the sorcerer (aril) has the effect of "going the wind and clearing the eye", taking the succulent skin, the saponin, the husk, the grass and the pudding, and the smashing The ball is large, and it can be used to wash the hair. It can also remove the eyesight. Very effective and other functions. In the early years of Taiwan, regardless of the flat or mountainous areas, there were all the trees in the whole land. The people picked up their fruits and used them to make soap after mashing. However, after the publication of the Qing (4), there was no interest in the harmlessness; however, excessive use of synthetic detergents It causes problems such as environmental pollution. In recent years, with the slogan of environmentally-friendly sounds and the side effects and toxicity of chemical products, the general consumer has generally raised the trend of advocating natural products. Therefore, the natural surfactants made by the scorpion and their related Products are highly valued. The chemical composition of the sapindus is complex, male head, enzyme, gum arabic, and saponin saponin. Among them, the saponins contained in the county are the most heavily weighted. This ingredient is not only known for cleaning. In addition to its usefulness, there are many biological activities. From the side; the chemical composition of the fruit is extremely complicated, taking the product made from the aqueous extract as an example. 'The product has poor product stability, the color is easy to blacken, and the acid and odor are produced. There are still many shortcomings in the use of harmless cleansing products, which is not a good designer, but needs to be improved. Commonly known as dandruff is actually a mild liposuction dermatitis that occurs in the scalp; many studies have pointed out that oval spleen, ® old corpse "·^°柳厂(10)(10) causes disease in liposuction dermatitis It plays an important role in the machine; it is a yeast-like fungus that is ubiquitous on the surface of human skin. The most distributed place is the lipid leaking part. If the scalp spores are detected on the scalp with dandruff The number of bacteria will be more than that of normal human scalp; in the shampoo that effectively treats dandruff on the market, it contains more or less the ingredients that can inhibit the sclerotium sclerotia, which shows the s. In the process of dandruff formation, 'must play an important role. 0 However, the inventors of this case, after two years of series of investigations and tests, showed that 'direct inhibition of the growth of B. sphaericus can only obtain the effect of palliative treatment. After a period of time, 'B. sphaericus may have inhibitors. Produces resistance, and reappears on the scalp of patients with dandruff; if the symptoms of dandruff are to be cured, it is appropriate to adjust the interface activity state of the surface layer of the dermatological skin, that is, to form a physiological environment that is less likely to cause liposuction dermatitis. Reduce the root cause of dandruff. In view of the above-mentioned disadvantages of the use of the sarcoid cleansing product and the conventional use of the dandruff shampoo, the inventor of the present invention has improved and innovated, and after years of painstaking research, he finally succeeded in researching and developing the sachet of the scalp. A method for preparing a crumb active material and a composition comprising the same. SUMMARY OF THE INVENTION An object of the present invention is to provide a dandruff-free active material and a composition comprising the same as a shampoo cleaning article capable of removing dandruff. The second purpose of the present invention is to provide a method for preparing a dandruff active Φ substance to produce a smear-free active substance which is excellent in stability, is not easily discolored, and deteriorates. In order to achieve the above object, the inventors of the present invention have a concept of different chemical composition and different components having different specific functions during the ripening of the fruit of the sapindus, and are further explored through long-time sampling detection for the lower polarity saponin. For the main extraction target, the green fruit (green fruit stage) was used as the raw material, and the aril of the seedless green fruit was treated with appropriate drying and pulverization, and then extracted with acetic acid acetonitrile, and the extract was decompressed. It is concentrated and dried to make an active ingredient. σ, 戎 product can adjust the surface activity of the scalp surface to form an environment where dandruff is less likely to occur. Therefore, the preparation method of the scorpion-free dandruff active material which can achieve the above object of the invention comprises the following steps: Step 1: Drying the fresh sachetberry fruit at 45 to 55 ° C for 2 to 4 days; 200944244 Step 2: Broken and dry helmet, ^ ... Moon fruit' sub-separation of its seeds and arils; v. 3 · Ethyl acetate solvent extraction of shai aril, the ratio of aril to ethyl acetate is 1/5 To 1/2 〇, vol. volume ratio), after the stroke, the extract and solid matter are separated by filtration or centrifugation; and 1 step 4: removing the ethyl acetate solvent contained in the extract by vacuum concentration, The extract obtained is a scorpion genus active substance.
❹ 一驟2之假種皮可進一步進行粉碎以製成粉末，並以真 空包裝儲存於常溫乾燥環境下；而該步驟4之無患子去頭皮屑 活性物質則可進-步進行冷;東乾燥並粉碎製成粉末，並密封儲 存於低溫環境下備用。 此外，本發明並提供一種用來清潔頭髮及去除頭皮屑之組 成物，忒組成物包含上述無患子去頭皮屑活性物質洗髮乳基 乳成分’以及其他洗髮乳可允許添加之微量成分。 其中該無患子去頭皮屑活性物質之含量為〇. 05至丨〇% (w/v);該洗髮乳基乳成分包含椰油硫酸鈉（s〇diufn c〇ceth sulfate)、甘油（Glycerol)、烧基醮胺甜菜驗（Cocamidopropyl betaine)、十二烧基硫酸銨（Ammonium lauryl sulfate)、玻尿 酸鈉（Sodium hyaluronate)、泛醇（D-Panthenol)、檸檬酸鈉 (Sodium citrate)、 瓜爾羥丙基氯化銨（Guar hydroxypropy 1 trimonium chloride)、乙^醇二石更月旨酉曼酉旨 (Ethyleneglycol disterate) ’ 以及二甲基石夕酮 200944244 (Dimethicone);該稀釋劑為水及其他液態組成份，例如：多元 醇類（如甘油該其他洗髮乳可允許添加之微量成分包括精油 (如：植物精油或微生物代謝物或合成香精等）及色素（包括色素 及色料）等。 【實施方式】 ※實施例一無患子去頭皮屑活性物質之製備 無患子去頭皮屑活性物質之製備流程如圖一所示；首先， 0 直接從無患子樹上採集新鮮無患子青果（翠綠色果實），將果實 顆粒摘取後，果實放置於45至55。〇之熱風乾燥箱中進行乾燥， 依放置量而定約需三天。將乾燥後無患子果實直接破碎，並將 脫落之種子過筛分離，再將假種皮進一步粉碎成粉末狀，隨即 以高密度ΡΕ塑膠袋真空密封包製後，即可倉儲作為全年萃取材 料。 請繼續參考圖一，進行無患子去頭皮屑活性物質萃取時， Ο 取則述無患子假種皮粉末與乙酸乙g旨以重量對體積比$ 1 / 5至 1/20之比率混合，再以攪拌機均質攪拌萃取（萃取溫度15至 30°C、時間為5至25分鐘）’並利用濾布過濾進行固液分離， 以去除固體殘/查，所知·之乙酸乙酯萃取液再進行減壓濃縮以去 除乙酸乙酯溶劑，所留下之乳黃色萃取物即為無患子去頭皮屑 活性物質；最後，將該活性物質凍結乾燥並粉碎，以製成粉末 狀無患子去頭皮屑活性物質產品，該產品密封後裝罐，進行低 10 200944244 溫（5 X以下）儲存，即可用以製造後續衍生產品。 A了確認以上述方式萃取之無患子去頭皮屑活性物質，是 否含有指標成分息素元’係以高效能管柱液相層析(Hlgh假 The seed coat of step 2 can be further pulverized to form a powder, and stored in a vacuum package in a normal temperature and dry environment; and the dandruff active material of the step 4 can be further cooled; the east is dried and pulverized. It is made into powder and sealed and stored in a low temperature environment for use. Further, the present invention provides a composition for cleaning hair and removing dandruff, the bismuth composition comprising the above-mentioned sarcoidic dandruff active shampoo base ingredient and other minor components which are allowed to be added by the shampoo. Wherein the content of the dandruff active substance is 〇. 05 to 丨〇% (w/v); the shampoo-based milk component comprises sodium sulphate (s〇diufn c〇ceth sulfate), glycerin (Glycerol) , Cocamidopropyl betaine, Ammonium lauryl sulfate, Sodium hyaluronate, D-Panthenol, Sodium citrate, Guar Guar hydroxypropy 1 trimonium chloride, Ethyleneglycol disterate ' and dimethyl ketone 200944244 (Dimethicone); the diluent is water and other liquids Group ingredients, such as: polyols (such as glycerin) other shampoos can allow the addition of trace components including essential oils (such as: plant essential oils or microbial metabolites or synthetic flavors) and pigments (including pigments and pigments). Embodiments] Example 1 Preparation of the dandruff active substance without scorpion preparation The preparation process of the dandruff active substance is shown in Fig. 1; firstly, 0 is collected directly from the sapindus tree. Green fruit (green fruit), after the fruit granules are removed, the fruit is placed at 45 to 55. The drying is carried out in a hot air drying oven, which takes about three days depending on the amount of the seed. The detached seeds are sieved and separated, and then the arils are further pulverized into powder form, and then vacuum sealed with a high-density ΡΕ plastic bag, which can be stored as a whole year's extraction material. Please continue to refer to Figure 1 for the dandruff to the dandruff In the extraction of the active substance, the sachet seed aril powder and the acetic acid are mixed in a ratio of weight to volume ratio of from 1 / 5 to 1 / 20, and then extracted by homogenization with a stirrer (extraction temperature of 15 to 30 ° C, The time is 5 to 25 minutes)' and the solid-liquid separation is carried out by filtration through a filter cloth to remove the solid residue/check. The ethyl acetate extract is known to be concentrated under reduced pressure to remove the ethyl acetate solvent. The milky yellow extract is the scorpion-free dandruff active substance; finally, the active substance is freeze-dried and pulverized to form a powdery scorpion-free dandruff active substance product, which is densely packed. After the can, it can be used to make the subsequent derivative products by storing the low 10 200944244 temperature (5 X or less). A. Confirming the extract of the dandruff active substance extracted in the above manner, whether it contains the indicator component Column column liquid chromatography (Hlgh
Performance Liquid Chromatography, ； 乂 Cl8 g柱ϋ 2G至㈣直線梯度甲醇溶劑系統進行分析，溶 劑流速及樣品注射量分別為1.0 mL/min & 20uL，管柱溫度 45 C，並W 220 nm波長監測，以及電腦積分儀進行數據分析建 參立圖譜樓案。無患子去頭皮屬活性物質之HpLC分析結果如圖二 所示，其各波鋒代表無患子系列皂素元及息素苦之組成分。※ 目前尚無完整標準品 ※實施例二含有無患子去頭皮屑活性物質洗髮乳之製備 取上述實施例一所製得之粉末狀無患子去頭皮屑活性物 質，添加至習用洗髮精基乳中，無患子去頭皮屑活性物質添加 之比例為0. 05至1 · 〇% (w/v)，經充分攪拌溶解後即為去頭皮屑 Ο 洗髮乳。該洗髮精基乳之配方如下： 含量（％) 純水（Pure water) 55-35 椰油硫酸納（Sodium coceth sul fate) 2-5 烧基醯胺甜菜鹼（Cocamidopropyl betaine) 1-3 甘油（Glycerol ) 0-10 十二烧基硫酸銨（Ammonium lauryl sulfate) 0.5-2 200944244 ±A(°/〇) 1-5 〇· 1-0. 5 〇· 1Ό. 5 成份 玻尿酸鈉（Sodium hyaluronate) 泛醇（即維他命D5，D-Panthenol) 檸檬酸納（Sodium citrate) 瓜爾羥丙基氣化銨 (Guar hydroxypropy1trimonium chloride) 1 乙二醇二硬脂酸酯（Ethyleneglycol disterate) 卜3 〇 二曱基石夕酮（Dimethicone) Q 5-2 5 ※實施例三去頭皮屑效果測試 . 取上述實施例二所製得之去頭皮屑洗髮乳進行去頭皮屑欵 果測試；先以具有頭皮屑困擾之志願者3人（男），長期（6個月） 試驗添加不同添加比率無患子活性物質之去頭皮屑洗髮乳的欵 果，以正常習性進行洗髮及撥動頭髮觀察皮屑掉落狀況。檢測 Ο方法為，每人改使用此洗髮乳前，先面對一張黑布或利用深色 桌面’再垂頭以右手用力抓頭撥動頭髮2G下，觀察調落之頭皮 屑之相對里，以5分代表此時使用前之狀況；之後的評分則為： 4分表不效果不明顯、3分表示已有效果、2分表示效果很明顯、 丁政果非常明顯、5分以上表示非但沒效果反而更嚴重。 、u生期自仃測試一次，結果顯示添加上述實施例一無患 子去頭皮屑活性物質usunu/v)的洗髮乳，連續使用一 12 200944244 星期後皆可達3分以下’之後因添加量及亦可能受生活作習及 飲食等因素，維持在1至3分之間，顯示均具有相當程度的去 ' 頭皮屑的效果。 接著，以添加0. 5% (w/v)無患子去頭皮屑活性物質之洗髮 乳’以及未添加無患子去頭皮屑活性物質之基乳作為試驗材 料，比較兩者去頭皮屑之效果；亦即受測者每次洗髮時，將頭 髮中分後，一邊以基乳洗頭，另一邊則以添加0.5% (w/v)無患 Φ 子去頭皮屑活性物質之洗髮乳洗頭；每天洗頭一次，連續一星 期後，分別撥動左右邊頭髮，以觀察比較兩邊頭皮屑掉落狀況， 除照相比較外（如圖三所示）’亦以前述檢測方法評量去頭皮屑 效果；檢測方法為，每人改使用此洗髮乳前，先面對—張黑布 或利用深色桌面，再垂頭以右手用力抓頭撥動頭髮2〇下，觀察 調洛之頭皮屑之相對量，以5分代表此時使用前之狀況·，之後 的評分則為：4分表示效果不明顯、3分表示已有效果、2分表 ©示效果很明顯、1分表示效果非常明顯、5分以上表示非但沒效 果反而更嚴重。結果顯示，各受測者以基乳洗頭那半邊的頭皮 屑量仍維持在5分，而以以添加〇5% (w/v)無患子去頭皮屬活 性物質之洗髮乳洗頭的另半邊頭皮屬量均達3分以下，顯示本 發明無患子去頭皮屑活性物質具有明顯減少頭皮屬量之效果。 另外，同樣以添加0.5% (w/v)無患子去頭皮屑活性物質之 洗髮乳作為試驗材料，以經徵詢同意具有頭皮屑困擾之志願者 13 200944244 11人（？男4女）為試驗對象，依其正常洗髮習性連續使用該洗 髮乳—-週’採前述相同檢測方法評量去頭皮屑效果；檢測方法 為母人改使用此洗髮乳前，先面對一張黑布或利用深色桌面， 再垂頭以右手用力抓頭撥動頭髮2〇下，觀察調落之頭皮屑之相 對量’以5分代表此時使用前之狀況；之後的評分則為：4分表 示效果不明顯、3分矣+ p 1 '、已有效果、2分表示效果很明顯、j分 41 ❹ 表示效果非常明顯、5分以上表示非但沒效果反而更嚴重。使用 -週後’8人即反映達評分為3分以下，有明顯減少頭皮屑量之 效果；連續使用二週後 1n k後10人反映評分達3分以下，有明顯減 少頭皮屑量之效果。 本發明所提供之無患子去頭皮屬活性物質其製備方法及含 彼組成物，與其他習用技術相互比較時，更具有下列之優點： 1.本發明所提供之含有無患子去頭皮屑活性物質之洗髮 孔，，㈣明顯減少頭皮肩產生量之效果，可提供洗髮乳（精）產 業製造向附加價值產品。 2.本發明所提供之益* j 土-s Α p ……ν"、子去碩皮屑活性物質之製備方 法’係以無患子新鮮青果為原料，所製備之乾燥粉末具有高去 碩皮屬活性、環保接受性，且為純植物性天然物、盈臭味、色 澤淡黃色m好⑽力W式嶋統之相容性 高’適合添加於多種洗髮乳(精)基乳，以增加洗髮乳⑷之功 效及價值。 14 200944244 、3.本發明所提供之無患子去頭皮屑活性物質之製備方 法，係包含無患子果實之前處理，果實經過乾燥、粉碎、製粉 及倉儲，可大量得到該活性物質之萃取原料，而該原料品質一 致、易於倉儲，且不受原料產期季節性之影響’可供全年萃取 之用，亦有利於產品之品管控制。 Ο 4’本發明所提供之無患子去頭皮屑活性物質之製備方 法，係以符合環保概念之乙酸乙醋進行萃取、減㈣縮及乾燥 製成粉末；產品無溶劑殘留之顧慮，而且回收之溶劑可循環使 用’有利降低加工成本。 上列詳細說明係針對本發明之一可行實施例之具體說明， 惟該實施例並非用以限制本發明之專利範圍，凡未脫離本發明 技藝精神所為之等效實施或變更，例如：洗髮乳（精）基乳成分 及含量等變化之等效性實施例，均應包含於本案之專利範圍中。 、T上所4本案不但在方法上確屬創新，並能較習用物品 曰進上述夕項功效’應已充分符合新穎性及進步性之法定發明 專利要件’爰依法提出申請，懇請t局核准本件發明專利申 請案’以勵發明’至感德便。 【圖式簡單說明】 圖； 圖一為本發明無患子去頭皮屑活性物質之製備方法之流程 圖譜；以及 圖二為無患子去頭皮屑活性物質之HPLC分析 15 200944244 圖三為左右頭髮頭皮屑之照相比較；受測者左邊（照片右 邊為基乳對照組）及受測者右邊（照#左邊為含活性物洗髮乳 實驗組）頭髮頭皮屑之照相比較，配合肉眼比較顯示洗基乳邊 之頭髮基部沾粘較多白色頭皮屑。 【主要元件符號說明】 無Performance Liquid Chromatography, ; 乂Cl8 g column ϋ 2G to (4) linear gradient methanol solvent system analysis, solvent flow rate and sample injection volume are 1.0 mL / min & 20 uL, column temperature 45 C, and W 220 nm wavelength monitoring, And the computer integrator for data analysis and construction of the map. The results of HpLC analysis of the scalp-free active substance of Sapindus are shown in Fig. 2, and the respective wave fronts represent the composition of the saponin and the saponin. ※ There is no complete standard product at present. ※Example 2 Preparation of the dandruff-free active substance shampoo. The powdered scorpion-free dandruff active substance obtained in the above Example 1 is added to the conventional shampoo base milk. The proportion of the dandruff active substance added is 0. 05 to 1 · 〇% (w/v), which is the anti-dandruff 洗 shampoo after being thoroughly stirred and dissolved. The shampoo base formula is as follows: Content (%) Pure water 55-35 Sodium coceth sul fate 2-5 Cocamidopropyl betaine 1-3 Glycerin (Glycerol) 0-10 Ammonium lauryl sulfate 0.5-2 200944244 ±A(°/〇) 1-5 〇· 1-0. 5 〇· 1Ό. 5 Ingredients Sodium hyaluronate Ubiquinol (ie vitamin D5, D-Panthenol) Sodium citrate Guar hydroxypropy 1trimonium chloride 1 Ethyleneglycol disterate 3 3 〇 曱 曱 曱Dimethicone Q 5-2 5 * Example 3 to dandruff effect test. Take the anti-dandruff shampoo prepared in the above Example 2 for dandruff test; first with dandruff Three volunteers (male), long-term (6 months) test the effect of adding dandruff shampoo with different ratios of Sapindus active substance, shampooing with normal habits and plucking hair to observe the dander drop condition. The method of detecting cockroaches is that before each person uses this shampoo, first face a black cloth or use a dark tabletop to slap the head and use the right hand to grab the head and shake the hair 2G to observe the relative dandruff of the dandruff. In the case, the score is 5 points for the situation before use; the subsequent scores are: 4 points are not effective, 3 points are already effective, 2 points are obvious, Ding Zhengguo is very obvious, 5 points or more It means that it is more serious but not effective. The test was carried out once in the u-life period. The results showed that the shampoo added with the above-mentioned Example 1 dandruff-free active material usunu/v) was used continuously for a period of 12, 2009, and 44 weeks. It may also be maintained between 1 and 3 points due to factors such as lifestyle and diet, showing a considerable degree of dandruff. Next, the effect of removing dandruff was compared with the addition of 0.5% (w/v) of the shampoo to the dandruff active substance and the base milk without the addition of the dandruff active substance as the test material. That is, each time the subject shampoos, the hair is divided, and the head is washed with the base milk, while the other side is shampooed with 0.5% (w/v) of the dandruff-free active ingredient. Wash your hair once a day, and after a week, move the left and right hairs separately to observe the dandruff drop on both sides. In addition to the photo comparison (as shown in Figure 3), the dandruff is also evaluated by the aforementioned detection method. The detection method is that before each person uses the shampoo, first face the black cloth or use the dark tabletop, then hang the head with the right hand and grab the hair to rub the hair 2 times, observe the dandruff of the Luo The relative amount is 5 points to represent the situation before use at this time. The subsequent score is: 4 points means the effect is not obvious, 3 points means the effect is already there, 2 points table is effective, 1 point means the effect is very Obviously, 5 points or more means that it is not more effective but more serious. The results showed that the amount of dandruff in the half of the basal shampoo was maintained at 5 points for each subject, and the shampoo was shampooed with 5% (w/v) of the scalp-free active substance. The amount of scalp in the half of the genus is less than 3 points, which shows that the dandruff active substance of the present invention has the effect of significantly reducing the amount of scalp. In addition, the shampoo with 0.5% (w/v) of the dandruff-free active substance was also used as the test material, and the volunteers who had been consulted for dandruff were invited to participate in the test. 2009 200944244 11 people (? male 4 female) According to the normal shampooing habits, the shampoo--weekly adopts the same detection method as mentioned above to evaluate the anti-dandruff effect; the detection method is that the mother uses a black cloth or a shampoo before using the shampoo. Use the dark tabletop, then use your right hand to grab the head and use your right hand to pull the hair 2 squats and observe the relative amount of dandruff in the smear. The score is 5 points to represent the situation before use. The subsequent score is: 4 points. The effect is not obvious, 3 points p + p 1 ', existing effect, 2 points means the effect is very obvious, j points 41 ❹ means the effect is very obvious, 5 points or more means not only the effect is more serious. After use-weeks, the score of 8 people is less than 3 points, which has the effect of significantly reducing the amount of dandruff; after 1 week of continuous use, 10 people reflect the score below 3 points after 1n k, which has the effect of reducing the amount of dandruff significantly. . The preparation method and the composition containing the sapindus genus of the sapindus genus provided by the invention have the following advantages when compared with other conventional techniques: 1. The invention provides the washing of the active substance containing the scorpion dandruff provided by the invention. Hair hole, (4) significantly reduces the amount of scalp shoulder production, can provide shampoo (fine) industry manufacturing to value-added products. 2. The benefits provided by the present invention are: j-s Α p ...... ν ", the preparation method of the sub-smear active material is based on the fresh green fruit of the saplings, and the prepared dry powder has a high genus Active, environmentally acceptable, and pure plant naturals, odor, light yellow color, good (10) force W, compatible with high-quality ' system, suitable for adding to a variety of shampoo (fine) base milk, to increase The efficacy and value of shampoo (4). 14 200944244, 3. The method for preparing the scorpion-free dandruff active material provided by the present invention comprises the treatment of the fruit of the sapindus, and the fruit is dried, pulverized, pulverized and stored, and the extracting material of the active substance is obtained in a large amount, and the raw material is obtained. Consistent quality, easy storage, and unaffected by the seasonality of raw materials. It is available for annual extraction and is also conducive to quality control of products. Ο 4' The preparation method of the scorpion-free dandruff active material provided by the present invention is carried out by extracting, reducing (four) shrinking and drying into a powder according to the environmental protection concept of ethyl acetate; the solvent-free residue of the product, and the solvent for recovery Recyclable' is beneficial to reduce processing costs. The detailed description of the present invention is intended to be illustrative of the preferred embodiments of the invention, and is not intended to limit the scope of the invention. Equivalent examples of changes in the composition and content of the milk (fine) base milk should be included in the patent scope of the present application. The 4 cases on T and T are not only innovative in terms of methods, but also can be applied to the above-mentioned eve items of the above-mentioned items in accordance with the customary items. This invention patent application 'invented the invention' to the sense of virtue. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a flow chart of a method for preparing a dandruff-free active substance of the present invention; and Figure 2 is an HPLC analysis of a dandruff-free active substance of the scorpion 15 200944244 Figure 3 is a photograph of the left and right hair dandruff Comparison; the left side of the subject (the right side of the photo is the control group) and the right side of the subject (pictured on the left side of the active shampoo containing experimental group), the dandruff was compared with the naked eye. The white base of the hair is more sticky with white dandruff. [Main component symbol description] None
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