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SI1737945T1 - Postopek za čiščenje plazmidne DNA - Google Patents

Postopek za čiščenje plazmidne DNA

Info

Publication number
SI1737945T1
SI1737945T1 SI200531262T SI200531262T SI1737945T1 SI 1737945 T1 SI1737945 T1 SI 1737945T1 SI 200531262 T SI200531262 T SI 200531262T SI 200531262 T SI200531262 T SI 200531262T SI 1737945 T1 SI1737945 T1 SI 1737945T1
Authority
SI
Slovenia
Prior art keywords
plasmid dna
purifying plasmid
purifying
dna
plasmid
Prior art date
Application number
SI200531262T
Other languages
English (en)
Inventor
Francis Blanche
Michel Couder
Nicolas Maestrali
Thierry Guillemin
David Gaillac
Original Assignee
Aventis Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP2004/011437 external-priority patent/WO2005026331A2/en
Application filed by Aventis Pharma Sa filed Critical Aventis Pharma Sa
Priority claimed from PCT/EP2005/005213 external-priority patent/WO2005100542A1/en
Publication of SI1737945T1 publication Critical patent/SI1737945T1/sl

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0091Purification or manufacturing processes for gene therapy compositions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00Specific treatment goals
    • C02F2303/06Sludge reduction, e.g. by lysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Epidemiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Manufacturing & Machinery (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Plant Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SI200531262T 2004-04-19 2005-04-19 Postopek za čiščenje plazmidne DNA SI1737945T1 (sl)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US56300804P 2004-04-19 2004-04-19
PCT/EP2004/011437 WO2005026331A2 (en) 2003-09-17 2004-09-17 Method of preparation of pharmaceutically grade plasmid dna
PCT/EP2005/005213 WO2005100542A1 (en) 2004-04-19 2005-04-19 Method for purifying plasmid dna
EP05739527A EP1737945B1 (en) 2004-04-19 2005-04-19 Method for purifying plasmid dna

Publications (1)

Publication Number Publication Date
SI1737945T1 true SI1737945T1 (sl) 2011-05-31

Family

ID=46045410

Family Applications (1)

Application Number Title Priority Date Filing Date
SI200531262T SI1737945T1 (sl) 2004-04-19 2005-04-19 Postopek za čiščenje plazmidne DNA

Country Status (4)

Country Link
EP (2) EP1737945B1 (sl)
CY (1) CY1111401T1 (sl)
PL (1) PL1737945T3 (sl)
SI (1) SI1737945T1 (sl)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3663401T3 (da) * 2019-02-28 2022-11-28 Lonza Ag Fremgangsmåde til rensning af biologiske molekyler såsom plasmid-dna ved hjælp af anionbytningskromatografi
EP4168545A4 (en) 2020-06-17 2024-07-17 Entegris Inc ION EXCHANGE MEMBRANES, FILTERS AND PROCESSES
BE1029550B1 (fr) * 2021-07-02 2023-01-30 Xpress Biologics Extraction plasmidique automatisée

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4129849A1 (de) 1991-02-14 1992-08-27 Boehringer Mannheim Gmbh Monoklonale antikoerper gegen humane pankreas-inselzellen
WO1996002658A1 (en) 1994-07-15 1996-02-01 Merck & Co., Inc. A method for large scale plasmid purification
FR2728264B1 (fr) 1994-12-16 1997-01-31 Rhone Poulenc Rorer Sa Purification d'adn par formation de triple helice avec un oligonucleotide immobilise
US7364894B2 (en) 1995-09-15 2008-04-29 Centelion Circular DNA molecule having a conditional origin of replication, process for their preparation and their use in gene therapy
US7279313B2 (en) 1995-09-15 2007-10-09 Centelion Circular DNA molecule having a conditional origin of replication, process for their preparation and their use in gene therapy
ATE268810T1 (de) 1995-12-21 2004-06-15 Genzyme Corp Methode zur zellyse
FR2746412B1 (fr) * 1996-03-21 1998-06-12 Rhone Poulenc Rorer Sa Purification d'adn plasmidique de qualite pharmaceutique
GB9613759D0 (en) 1996-07-01 1996-09-04 De La Rue Systems Ltd Apparatus for monitoring a document
US6214586B1 (en) 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
US6441160B2 (en) 1998-05-11 2002-08-27 Tosoh Corporation Separating plasmids from contaminants using hydrophobic or hydrophobic and ion exchange chromatography
AU2001263459B2 (en) * 2000-05-26 2007-03-15 Centelion S.A.S. Purification of a triple heli formation with an immobilized oligonucleotide
NZ528507A (en) 2001-03-23 2005-08-26 Centelion S Processes for purifying and for detecting target double-stranded DNA sequences by triple helix interaction

Also Published As

Publication number Publication date
EP2246413A2 (en) 2010-11-03
CY1111401T1 (el) 2015-08-05
EP1737945A1 (en) 2007-01-03
EP1737945B1 (en) 2011-01-26
PL1737945T3 (pl) 2011-07-29
EP2246413A3 (en) 2011-10-26

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