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RU2396337C2 - METHOD FOR OBTAINING L-THREONINE USING Escherichia GENUS BACTERIA IN WHICH yncD GENE IS INACTIVATED - Google Patents

METHOD FOR OBTAINING L-THREONINE USING Escherichia GENUS BACTERIA IN WHICH yncD GENE IS INACTIVATED Download PDF

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RU2396337C2
RU2396337C2 RU2007128314/13A RU2007128314A RU2396337C2 RU 2396337 C2 RU2396337 C2 RU 2396337C2 RU 2007128314/13 A RU2007128314/13 A RU 2007128314/13A RU 2007128314 A RU2007128314 A RU 2007128314A RU 2396337 C2 RU2396337 C2 RU 2396337C2
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threonine
obtaining
inactivated
gene
genus bacteria
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RU2007128314/13A
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Russian (ru)
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RU2007128314A (en
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Валерий Васильевич Самсонов (RU)
Валерий Васильевич Самсонов
Любовь Юрьевна Горбачёва (RU)
Любовь Юрьевна Горбачёва
Валерий Завенович Ахвердян (RU)
Валерий Завенович Ахвердян
Эльвира Борисовна Ворошилова (RU)
Эльвира Борисовна Ворошилова
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Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ)
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Priority to RU2007128314/13A priority Critical patent/RU2396337C2/en
Priority to PCT/JP2008/063722 priority patent/WO2009014259A1/en
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
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    • C12P13/12—Methionine; Cysteine; Cystine
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/20—Aspartic acid; Asparagine
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/227—Tryptophan
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/24—Proline; Hydroxyproline; Histidine

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  • Chemical & Material Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to biotechnology and is a method of obtaining L-threonine using Escherichia genus bacteria, modified in such a way that the yncD gene in the said bacteria is inactive.
EFFECT: invention enables highly efficient production of L-threonine.
3 cl, 2 dwg, 2 tbl, 11 ex

Description

Текст описания приведен в факсимильном виде.

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The text of the description is given in facsimile form.
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Claims (3)

1. Бактерия, принадлежащая к роду Escherichia, - продуцент L-треонина, модифицированная таким образом, что в указанной бактерии инактивирован ген yncD.1. A bacterium belonging to the genus Escherichia is a producer of L-threonine, modified in such a way that the yncD gene is inactivated in this bacterium. 2. Бактерия по п.1, отличающаяся тем, что указанный ген yпcD инактивирован за счет делеции гена yncD в хромосоме бактерии.2. The bacterium according to claim 1, characterized in that said ypcD gene is inactivated due to deletion of the yncD gene in the bacterial chromosome. 3. Способ получения L-треонина, включающий:
выращивание бактерии по любому из пп.1 и 2 в питательной среде, вызывающее продукцию и накопление L-треонина в культуральной жидкости; и
выделение L-треонина из культуральной жидкости. 3. A method of obtaining L-threonine, including:
growing bacteria according to any one of claims 1 and 2 in a nutrient medium, causing production and accumulation of L-threonine in the culture fluid; and
isolation of L-threonine from the culture fluid.
RU2007128314/13A 2007-07-24 2007-07-24 METHOD FOR OBTAINING L-THREONINE USING Escherichia GENUS BACTERIA IN WHICH yncD GENE IS INACTIVATED RU2396337C2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
RU2007128314/13A RU2396337C2 (en) 2007-07-24 2007-07-24 METHOD FOR OBTAINING L-THREONINE USING Escherichia GENUS BACTERIA IN WHICH yncD GENE IS INACTIVATED
PCT/JP2008/063722 WO2009014259A1 (en) 2007-07-24 2008-07-24 A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE yncD GENE

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Application Number Priority Date Filing Date Title
RU2007128314/13A RU2396337C2 (en) 2007-07-24 2007-07-24 METHOD FOR OBTAINING L-THREONINE USING Escherichia GENUS BACTERIA IN WHICH yncD GENE IS INACTIVATED

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RU2396337C2 true RU2396337C2 (en) 2010-08-10

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CN111647057B (en) * 2020-05-07 2022-09-13 西南医科大学附属中医医院 Salmonella typhi vaccine recombinant protein, coding gene thereof and application thereof

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DE10303571A1 (en) * 2003-01-30 2004-08-12 Degussa Ag Process for the fermentative production of L-amino acids using strains of the Enterobacteriaceae family
WO2007086618A1 (en) * 2006-01-30 2007-08-02 Ajinomoto Co., Inc. L-amino acid producing bacterium and method of producing l-amino acid

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