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RU2396349C2 - METHOD OF OBTAINING L-THREONINE USING Escherichia GENUS BACTERIA IN WHICH ygiD GENE IS INACTIVATE - Google Patents

METHOD OF OBTAINING L-THREONINE USING Escherichia GENUS BACTERIA IN WHICH ygiD GENE IS INACTIVATE Download PDF

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Publication number
RU2396349C2
RU2396349C2 RU2007112271/13A RU2007112271A RU2396349C2 RU 2396349 C2 RU2396349 C2 RU 2396349C2 RU 2007112271/13 A RU2007112271/13 A RU 2007112271/13A RU 2007112271 A RU2007112271 A RU 2007112271A RU 2396349 C2 RU2396349 C2 RU 2396349C2
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RU
Russia
Prior art keywords
threonine
ygid
gene
obtaining
inactivate
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RU2007112271/13A
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Russian (ru)
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RU2007112271A (en
Inventor
Константин Вячеславович Рыбак (RU)
Константин Вячеславович Рыбак
Эльвира Борисовна Ворошилова (RU)
Эльвира Борисовна Ворошилова
Михаил Маркович ГУСЯТИНЕР (RU)
Михаил Маркович Гусятинер
Original Assignee
Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ)
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Priority to RU2007112271/13A priority Critical patent/RU2396349C2/en
Publication of RU2007112271A publication Critical patent/RU2007112271A/en
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Abstract

FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to biotechnology and is a method of obtaining L-threonine using Escherichia genus bacteria, modified in such a way that the ygiD gene in the said bacteria is inactive.
EFFECT: invention enables highly efficient production of L-threonine.
3 cl, 2 dwg, 2 tbl, 11 ex

Description

Текст описания приведен в факсимильном виде.

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The text of the description is given in facsimile form.
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Claims (3)

1. Бактерия, принадлежащая к роду Escherichia, - продуцент L-треонина, модифицированная таким образом, что в указанной бактерии инактивирован ген ygiD.1. A bacterium belonging to the genus Escherichia is a producer of L-threonine, modified in such a way that the ygiD gene is inactivated in this bacterium. 2. Бактерия по п.1, отличающаяся тем, что указанный ген ygiD инактивирован за счет делеции гена ygiD в хромосоме бактерии.2. The bacterium according to claim 1, characterized in that said ygiD gene is inactivated due to deletion of the ygiD gene in the bacterial chromosome. 3. Способ получения L-треонина, включающий:
выращивание бактерии по любому из пп.1 и 2 в питательной среде, вызывающее продукцию и накопление L-треонина в культуральной жидкости; и
выделение L-треонина из культуральной жидкости. 3. A method of obtaining L-threonine, including:
growing bacteria according to any one of claims 1 and 2 in a nutrient medium, causing production and accumulation of L-threonine in the culture fluid; and
isolation of L-threonine from the culture fluid.
RU2007112271/13A 2007-04-03 2007-04-03 METHOD OF OBTAINING L-THREONINE USING Escherichia GENUS BACTERIA IN WHICH ygiD GENE IS INACTIVATE RU2396349C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2007112271/13A RU2396349C2 (en) 2007-04-03 2007-04-03 METHOD OF OBTAINING L-THREONINE USING Escherichia GENUS BACTERIA IN WHICH ygiD GENE IS INACTIVATE

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2007112271/13A RU2396349C2 (en) 2007-04-03 2007-04-03 METHOD OF OBTAINING L-THREONINE USING Escherichia GENUS BACTERIA IN WHICH ygiD GENE IS INACTIVATE

Publications (2)

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RU2007112271A RU2007112271A (en) 2008-10-10
RU2396349C2 true RU2396349C2 (en) 2010-08-10

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Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MIGUEL A. VALVANO ET. AL. The rfaE Gene from Escherichia coli Encodes a Bifunctional Protein Involved in Biosynthesis of the Lipopolysaccharide Core Precursor ADP-1-glycero-d-manno-Heptose J Bacteriol. 2000 January; 182(2): 488-497. *

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