KR20220010919A - Cosmetic composition for improving skin wrinkle comprising extract of crataegus pinnatifida fruit - Google Patents

Abstract

The present invention provides a cosmetic composition which comprises a Crataegus pinnatifida fruit extract or a separation compound thereof as an active ingredient and has an excellent effect in alleviating skin wrinkles through effects of increasing fibroblast proliferation, increasing cytochrome C oxidase, and increasing ATP biosynthesis.

Description

COSMETIC COMPOSITION FOR IMPROVING SKIN WRINKLE COMPRISING EXTRACT OF CRATAEGUS PINNATIFIDA FRUIT comprising hawthorn fruit extract as an active ingredient

The present invention contains hawthorn ( Crataegus Pinnatifida ) fruit extract, or an isolated compound thereof, ursolic acid and / or beta-sitosterol as an active ingredient, cytochrome C oxidase (cyhtochrome C oxidase) It relates to a cosmetic composition having an effect of improving skin wrinkles by increasing and increasing ATP biosynthesis.

Human skin undergoes various physical and chemical changes during the aging process. The causes are largely divided into intrinsic aging and photo-aging, and studies on these have been actively conducted. Free radicals are activated and caused by UV rays, stress, disease states, environmental factors, wounds, and aging. and accelerate aging. More specifically, lipids, proteins, polysaccharides, nucleic acids, etc., which are major constituents of the skin, are oxidized, and skin cells and tissues are destroyed, resulting in skin aging.

A person's skin color is determined by melanin, hemoglobin, and carotene, among which melanin plays the most important role. In addition to determining a person's skin color, melanin also performs skin protective actions such as UV absorption and free radical scavengers. However, when melanin is excessively generated by external environmental changes such as overexposure to ultraviolet rays, air pollution, stress, etc., it causes pigmentation in the skin, which causes skin blackening, blemishes, freckles, and the like. Blackening of the skin is caused by the reaction of skin cells to internal and external factors, and a representative factor is due to UV exposure. That is, when the skin is exposed to ultraviolet rays, tyrosinase is activated. By the oxidation process in which the tyrosinase acts on tyrosine present in the skin tissue to generate DOPA and dopaquinone, the skin A polymer called melanin is synthesized in melanosomes in melanocytes, which are pigment cells, and this melanin is transferred to keratinocytes, which are keratinocytes of the skin, and reaches the skin surface through keratinization to protect the skin from UV rays. Therefore, melanin is an essential UV protection agent for our human body, and also plays the role of an effective free radical scavenger that removes various radicals that modify biocomponents such as proteins, lipids, and nucleic acids.

In particular, the oxidation of proteins cuts the connective tissues of the skin, such as collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin, causing severe hyperinflammatory reactions and disruption of skin elasticity. And if it gets worse, it leads to mutations, cancer induction, and decreased immune function due to DNA mutations. Therefore, it is necessary to protect the cell membrane by removing free radicals generated during the body's metabolic process, UV irradiation, and free radicals mediated by inflammatory reactions, and regenerate damaged cells through active metabolism to proliferate the cells. The skin can recover quickly and maintain healthy skin.

In aging, not only free radicals but also an enzyme called Matrix Metalloproteinase (MMP), an enzyme that degrades collagen, are also involved. That is, the synthesis and degradation of extracellular matrix, such as collagen, is appropriately regulated in vivo, but as aging progresses, collagen synthesis decreases, and the expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen, is promoted, so that the skin The elasticity of the skin is reduced and wrinkles are formed. In addition, these decomposing enzymes are also activated by ultraviolet irradiation. Therefore, there is a need for the development of substances capable of regulating MMP expression induced in activation or inhibiting the activity in cells.

Oxygen, along with water, is an indispensable substance for our lives, but once it enters our body, it turns into highly active oxygen for various reasons and oxidizes blood vessel cells. Today, about 90% of the causes of disease have been identified as due to these free radicals. The human body is composed of about 60 trillion cells, and the cell membrane is formed of the lymphoid cortex, so it is easy to connect free radicals. When oxidation occurs, unsaturated fatty acids are converted into lipid peroxides. This lipid peroxide damages genetic DNA, RNA, proteins, cell membranes and cell structures, leading to cancer or various adult diseases, ultimately leading to aging. Substances that prevent cell oxidation by removing or neutralizing these free radicals are called antioxidants. In general, when peroxidation occurs due to free radicals in lipids, which are components of skin cells, the structure of the cell membrane is modified, causing cell permeability, fluidity, inactivation of membrane enzymes and carrier proteins, cleavage of protein chains, DNA and RNA damage, etc. It is known that skin cells are aging. For this reason, efforts to remove active oxygen continued, and as a result of this, many sulfated substances were developed. Substances known to have antioxidant activity so far include ascorbic acid, beta-carotene, dibutylhydroxyltoluene (BHT), and DL-α-tocopherol. .

To date, various substances as described above have been developed to suppress skin aging, and recently, skin care devices such as high-frequency devices, ultrasonic devices, galvanic current devices, and lifting devices have been developed to help skin aging. These devices are known to induce cell restoration and healing by increasing the production of adenosine triphosphate (ATP) by supplying electric energy of a microcurrent to the cells. The present inventors have completed the present invention through the study of ATP production promoting materials derived from natural products.

A number of patents on cosmetic compositions having an effect of improving skin wrinkles, including plant extracts, have been published. Korean Patent Registration No. 10-1574248 discloses a cosmetic composition for improving skin wrinkles comprising an extract of Reynoutria sachalinensis as an active ingredient. In addition, Korean Patent Registration No. 10-1693931 discloses a cosmetic composition for improving wrinkles comprising an extract of Scytosiphon lomentaria as an active ingredient.

As a result of searching for various skin physiological activities for natural plants and their isolated compounds, the present inventors found that among natural plant extracts, Hawthorn ( Crataegus Pinnatifida ) fruit extract and its isolated compounds ursolic acid and beta-sitosterol (beta-sitosterol) ) from cytochrome C oxidase activity increase and ATP generation efficacy was confirmed and completed the present invention.

The above object is achieved in a cosmetic composition for improving skin wrinkles comprising an extract of a hawthorn ( Crataegus Pinnatifida ) fruit or an isolated compound thereof, ursolic acid or beta-sitosterol as an active ingredient.

Preferably, the cosmetic composition has an ATP biosynthesis promoting effect or cytochrome C oxidase activity promoting effect.

Also preferably, the ursolic acid may be contained in an amount of 0.0001 to 0.01 wt%.

Also preferably, the beta-sitosterol may be contained in an amount of 0.0001 to 0.01% by weight.

Also preferably, the cosmetic composition may include ursolic acid and beta-sitosterol in a weight ratio of 1:1 to 1:10.

Also preferably, the cosmetic composition is selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. It is characterized in that it has a selected formulation.

The cosmetic composition of the present invention contains an extract of Crataegus Pinnatifida fruit or an isolated compound thereof, ursolic acid, beta-sitosterol, or a mixture thereof, characterized in that it has an ATP production promoting effect. . The active ingredient, Crataegus Pinnatifida fruit extract, contained in the cosmetic composition of the present invention, provides a wrinkle improvement effect on the skin by having a fibroblast proliferation effect, an increase in cytochrome C oxidase activity, and an ATP generation effect.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and have the meanings as commonly understood by one of ordinary skill in the art of the present invention. In addition, although preferred methods and samples are described herein, similar or equivalent ones are also included in the scope of the present invention.

The term "about" means 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length varying by 4, 3, 2 or 1%.

Throughout this specification, unless the context requires otherwise, the terms "comprises" and "comprising" include, but are not limited to, a given step or element, or group of steps or elements, but any other step or element, or It is to be understood that a step or group of elements is not excluded.

The present invention relates to a cosmetic composition comprising, as an active ingredient, a hawthorn ( Crataegus Pinnatifida ) fruit extract or its isolated ?d water, ursolic acid or beta-sitosterol.

The hawthorn ( Crataegus Pinnatifida ) used in the present invention is a deciduous broad-leaved tree in the Rosaceae family of Rosaceae, which grows in a place with abundant sunlight. As hawthorn, it was used to treat indigestion, enteritis, and back pain.

The Hawthorn ( Crataegus Pinnatifida ) fruit extract of the present invention may be prepared according to a conventional method known in the art, that is, under ordinary temperature and pressure conditions, using a conventional solvent, but preferably (a ) water, anhydrous or hydrous lower alcohols having 1-4 carbon atoms (e.g., methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl Solvent extraction using a solvent selected from the group consisting of acetate, dichloromethane, chloroform, hexane, and mixtures thereof (b) supercritical extraction using reduced pressure and high temperature with carbon dioxide or (c) ultrasonic extraction. .

In the present invention, preferably, a solvent extraction method using an extraction solvent was used. The extraction solvent is, for example, water, anhydrous or hydrous lower alcohols having 1-4 carbon atoms (eg, methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, At least one extraction solvent selected from the group consisting of diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane and mixtures thereof may be used, preferably ethanol, 70% (v/v) ethanol or It is obtained using water, and most preferably is obtained using 70% (v/v) ethanol.

On the other hand, it is apparent to those skilled in the art that, for the fruit extract of Crataegus Pinnatifida of the present invention, an extract exhibiting substantially the same effect can be obtained using other extraction solvents as well as the above-described extraction solvent. Decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by extraction using ultrasound, separation using an ultrafiltration membrane with a constant molecular weight cut-off value, various chromatography (depending on size, charge, hydrophobicity or affinity) The active fraction obtained through the extraction method for separation by the one prepared for separation) is also included in the extract of the present invention.

The ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the active ingredient as an active ingredient, for example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances. , and a carrier. The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether as carrier components Sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester may be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , cleansing lotion, cleansing water, and cleansing gel, but not limited thereto.

On the other hand, the makeup method of the present invention refers to all makeup methods using the cosmetic composition of the present invention. That is, all methods known in the art using the cosmetic composition belong to the cosmetic method of the present invention. The cosmetic composition of the present invention may be used alone or in combination, or may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the skin condition or taste of the user.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples according to the gist of the present invention.

In the present invention, ursolic acid, an isolated compound isolated from the fruit extract of Crataegus Pinnatifida , is shown in Formula 1 below, and beta-sitosterol is shown in Formula 2.

[Formula 1] Ursolic acid

[Formula 2] beta-sitosterol (beta-sitosterol)

In the present invention, "extract" is an extract obtained from hawthorn ( Crataegus Pinnatifida ) fruit using a solvent such as water or ethanol, and a diluted or concentrated solution of the extract, a dried product obtained by drying it, and a prepared or purified product of the extract It refers to the extract of all formulations that can be formed using the extract, etc.

Preparation Example 1 - Hawthorn ( Crataegus Pinnatifida ) Preparation of fruit extract

After washing 10 kg of hawthorn fruit with clean water, drying it, pulverize it to a constant (100 mesh) size, extract under reflux three times for 5 hours with 70% (V/V) ethanol aqueous solution, cool, and then cool with Whatman #4 filter paper 2.1 kg of hawthorn fruit extract was prepared by filtration using a concentrator.

Preparation Example 2 - Preparation of ursolic acid

After dispersing 10 L of 5% ethanol in 1.0 kg of the hawthorn fruit extract obtained in Preparation Example 1, the non-polar component was removed using 10 L of hexane (repeated 3 times), and then the fractionation layer fractionated with methylene chloride was concentrated under reduced pressure to obtain a fraction of 35 g. The active ingredient was separated using a silica gel (60 ~ 120mesh) column, and the mobile phase was separated and purified using a CH2Cl2-EtOAc mixture. A total of 15 fractions were recovered, and 1.55 g was recovered from one fraction having a cytochrome C oxidase promoting effect, and was separated and identified using NMR. By comparing the NMR results with literature data and standard products (cas no: 77-51-1, ursolic acid), it was confirmed that the isolated and identified component was ursolic acid (LABIB, Rola M., et al. Ursolic acid, a natural pentacylcic triterpene from Ochrosia elliptica and its role in the management of certain neglected tropical diseases. Pharmacognosy magazine, 2016, 12.48: 319). Ursolic acid was isolated from Preparation Example 2, and its specific chemical formula is shown in Formula 1 above.

Preparation 3 - Preparation of beta-sitosterol (β-sitosterol)

After dispersing 10 L of 5% ethanol in 1.0 kg of the hawthorn fruit extract obtained in Preparation Example 1, the non-polar component was removed using 10 L of hexane (repeated 3 times), and then the fractionation layer fractionated with methylene chloride was concentrated under reduced pressure to obtain a fraction of 35 g. The active ingredient was separated using a silica gel (60 ~ 120mesh) column, and the mobile phase was separated and purified using a CH2Cl2-EtOAc mixture. A total of 15 fractions were recovered, and 0.21 g was recovered from one fraction having a cytochrome C oxidase promoting effect, and separated and identified using NMR. By comparing the NMR results with literature data and with standard products (cas no: 83-46-5, β-Sitosterol), it was confirmed that the isolated and identified component was beta-sitosterol (ODODO, Mesfin Medihin; CHOUDHURY, Manash Kumar; DEKEBO, Ahmed). Hussen. Structure elucidation of β-sitosterol with antibacterial activity from the root bark of Malva parviflora ( SpringerPlus, 2016, 5.1: 1-11.). Beta sitosterol (β-sitosterol) was isolated from Preparation Example 3, and its specific chemical formula is shown in Formula 2 above.

Experimental Example 1 - Cell proliferation effect

Human-derived fibroblasts were cultured using DMEM medium containing 10% FBS and 1% antibiotics (100 units/ml penicillin and 100 ug/ml streptomycin). The growth and proliferation efficacy of fibroblasts was confirmed by MTT assay using the fibroblasts of the 3rd and 4th passage. ^{Fibroblasts (1×10 4} cells/ml) were put in a DMEM medium containing 1% FBS in a 96-well microplate and pre-cultured for 24 hours, and the samples of Preparation Examples 1 and 2 were treated at various concentrations, and then cultured for 4 days. did

50 μl of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was added, incubated for another 4 hours, and then centrifuged to remove the supernatant. Herein, dimethylsulfoxide was added to dissolve the precipitate, and the absorbance was measured at 540 nm to confirm the proliferation effect of human-derived fibroblasts to the extent of the formation of a formazan precipitate according to the reduction of MTT. The results are shown in Tables 1 and 2.

Sample name Concentration (ppm) Cell proliferation effect (%) (vs. control) Preparation Example 1 100 120.74% ± 1.07 10 101.07% ± 1.42 One 98.84% ± 2.08 Preparation 2 50 127.34% ± 1.10 10 119.46% ± 1.42 One 105.82% ± 1.12 Preparation 3 50 105.43% ± 0.70 10 99.75% ± 1.33 One 99.10 % ± 0.89

Sample name and concentration (ppm) Cell proliferation effect (%) (vs. control) Preparation 2
(1ppm) Preparation 3
(10ppm) 130.00% ± 1.69 Preparation 3
(1ppm) 115.27% ± 2.44

Experimental Example 2 - Cytochrome C oxidase activity

Human-derived fibroblasts were cultured using DMEM medium containing 10% FBS and 1% antibiotics (100 units/ml penicillin and 100 ug/ml streptomycin). The fibroblasts of the 3rd and 4th passage were used for the experiment. ^{Fibroblasts (1×10 4} cells/ml) were placed in a DMEM medium containing 1% FBS in a 96-well microplate, pre-cultured for 24 hours, and the samples obtained from Preparation Example were treated at various concentrations, and then cultured for 4 days. The cells were harvested, washed 2-3 times with PBS, 500 μl of lysis buffer was added, lysed for 1 hour, and then centrifuged at 12,000 rpm for 15 minutes to obtain a supernatant, and the protein concentration was BSA (bovine serum). albumin) was used as a standard material and quantified using Protein Assay Kit (BIO-RAD, HC, USA). The activity of cytochrome c oxidase was measured using the Cytochrome C Oxidase Assay Kit (ab239711). The degree of cytochrome c oxidase activity is shown in Tables 3 and 4 below as compared to the untreated group.

Sample name Concentration (ppm) Cydochrome C oxidase activity increase rate (%)
(compared to the untreated group) Preparation Example 1 100 15.59% ± 0.97 50 9.47 % ± 0.83 10 3.02% ± 0.44 One - Preparation 2 50 19.25% ± 0.57 10 10.54% ± 0.70 One 5.17% ± 0.93 Preparation 3 50 10.52% ± 1.01 10 4.85% ± 0.67 One 0.55% ± 0.27

Sample name and concentration (ppm) Cydochrome C oxidase activity increase rate (%)
(compared to the untreated group) Preparation 2
(1ppm) Preparation 3
(10ppm) 14.19% ± 1.01 Preparation 3
(1ppm) 9.55 % ± 0.90

Experimental Example 3 - ATP biosynthesis amount

Human-derived fibroblasts were cultured using DMEM medium containing 10% FBS and 1% antibiotics (100 units/ml penicillin and 100 ug/ml streptomycin). The fibroblasts of the 3rd and 4th passage were used for the experiment. ^{Fibroblasts (1×10 4} cells/ml) were put in a DMEM medium containing 1% FBS in a 96-well microplate, pre-cultured for 24 hours, and the samples obtained in Preparation Example were treated with various concentrations and then cultured for 4 days. The cells were harvested, washed 2-3 times with PBS, 500 μl of lysis buffer was added, lysed for 1 hour, and then centrifuged at 12,000 rpm for 15 minutes to obtain a supernatant, and the protein concentration was BSA (bovine serum). albumin) was used as a standard material and quantified using Protein Assay Kit (BIO-RAD, HC, USA). ATP biosynthesis was measured using ViaLight Plus Cell Proliferation and Cytotoxicity BioAssay Kit. The amount of generated ATP is shown in Tables 5 and 6 below in comparison with the untreated group.

Concentration (ppm) ATP increase rate
(compared to the untreated group) Preparation Example 1 100 10.15% 50 5.08% 10 1.47% One - Preparation Example 2 50 23.97% 10 11.10% One 5.38% Preparation 3 50 14.96% 10 5.17% One 2.44%

Sample name and concentration (ppm) ATP increase rate
(compared to the untreated group) Preparation 2
(1ppm) Preparation 3
(10ppm) 14.05% Preparation 3
(1ppm) 10.75%

Prescription Example 1 - Cream

Prescription examples of creams among cosmetics containing the hawthorn fruit extract prepared through the above preparation examples and preparation examples containing the active ingredients thereof are shown in Table 7 below, and are not limited to this formulation example. More specifically, it can be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a shampoo, a treatment, a scalp ampoule, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder. have

Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 content
(weight%) Preparation Example 1



glycerin
betaine
Fiji 1500
allantoin
DL-Panthenol
E.D.T.A-2Na
Benzophenone - 9
hydroxyethyl
cellulose
Sodium Hyaluronate
Carboxyvinyl Polymer
triethanolamine
Octyldodecanol
Octyldodeces -16
preservatives, fragrances, colors
Distilled water Preparation Example 2



glycerin
betaine
Fiji 1500
allantoin
DL-Panthenol
E.D.T.A-2Na
Benzophenone - 9
hydroxyethyl
cellulose
Sodium Hyaluronate
Carboxyvinyl Polymer
triethanolamine
Octyldodecanol
Octyldodeces -16
preservatives, fragrances, colors
Distilled water Preparation 3



glycerin
betaine
Fiji 1500
allantoin
DL-Panthenol
E.D.T.A-2Na
Benzophenone - 9
hydroxyethyl
cellulose
Sodium Hyaluronate
Carboxyvinyl Polymer
triethanolamine
Octyldodecanol
Octyldodeces -16
preservatives, fragrances, colors
Distilled water Preparation Example 2+3
(ursolic acid, β-Sitosterol 1ppm each)
glycerin
betaine
Fiji 1500
allantoin
DL-Panthenol
E.D.T.A-2Na
Benzophenone - 9
hydroxyethyl
cellulose
Sodium Hyaluronate
Carboxyvinyl Polymer
triethanolamine
Octyldodecanol
Octyldodeces -16
preservatives, fragrances, colors
Distilled water One



3.0
10.0
5.0
2.0
0.1
0.3
0.02
0.04

0.1
8.0
0.2
0.18
6.0
a very small amount
remaining amount Sum 100

Example 1 : Wrinkle improvement effect

Formulation Examples 1 to 4 containing the hawthorn fruit extract and its active ingredient and Comparative Examples in which the active ingredient was replaced with purified water were evaluated. 100 women aged 30-40 years were randomly divided into 5 groups, and the creams of Formulation Examples 1 to 4 and Comparative Formulation Example 1 were washed twice every morning and evening, and an appropriate amount of the cream was applied continuously for 1 month, centered on both cheeks. The skin elasticity improvement effect of each subject was evaluated using a Cutometer (C+K, Germany) to evaluate the elasticity improvement effect. The experimental results are as shown in Table 8 below.

division before use 2 weeks of use 4 weeks after use Formulation Example 1 20.44 24.35 25.13 Formulation Example 2 21.37 25.02 27.79 Formulation Example 3 19.11 20.41 21.54 Formulation Example 4 19.57 24.75 30.07 Comparative Formulation Example 1 22.05 23.49 22.98

Claims (7)

Hawthorn ( Crataegus Pinnatifida ) A cosmetic composition for skin wrinkle improvement comprising an extract or an isolated compound thereof as an active ingredient. The cosmetic composition for improving skin wrinkles according to claim 1, wherein the separation compound is ursolic acid or beta-sitosterol. The cosmetic composition for improving skin wrinkles according to claim 1, wherein the cosmetic composition has an ATP biosynthesis promoting effect or cytochrome C oxidase activity promoting effect. The cosmetic composition for improving skin wrinkles according to claim 2, wherein the ursolic acid is contained in an amount of 0.0001 to 0.01 wt%. The cosmetic composition for improving skin wrinkles according to claim 2, wherein the beta-sitosterol is contained in an amount of 0.0001 to 0.01 wt%. The cosmetic composition for improving skin wrinkles according to claim 2, wherein the cosmetic composition contains ursolic acid and beta-sitosterol in a weight ratio of 1:1 to 1:10. The group of claim 1, wherein the cosmetic is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray. A cosmetic composition for improving skin wrinkles, characterized in that it has a formulation selected from